| RepC protein of the octopine-type Ti plasmid binds to the probable origin of replication within repC and functions only in cis. | Vegetative replication and partitioning of many plasmids and some chromosomes of alphaproteobacteria are directed by their repABC operons. RepA and RepB proteins direct the partitioning of replicons to daughter cells, while RepC proteins are replication initiators, although they do not resemble any characterized replication initiation protein. Here we show that the replication origin of an Agrobacterium tumefaciens Ti plasmid resides fully within its repC gene. Purified RepC bound to a site with ... | 2011 | 21883520 |
| supramolecular complexes of the agrobacterium tumefaciens virulence protein vire2. | virulence protein vire2 from agrobacterium tumefaciens is involved in plant infection by transferring a fragment of agrobacterial ti plasmid sst-dna in complex with vire2-vird2 proteins into the plant cell nucleus. the vire2 protein interactions with ssdna and formation of vire2 protein complexes in vitro and in silico have been studied. using dynamic light scattering we found that purified recombinant protein vire2 exists in buffer solution in the form of complexes of 2-4 protein molecules of 1 ... | 2011 | 22117554 |
| Random insertional mutagenesis in fungal genomes to identify virulence factors. | Agrobacterium tumefaciens-mediated transformation (ATMT) has become an important tool for functional genomics in fungi. ATMT-based approaches such as random insertional mutagenesis and targeted knockout are widely used for gene functional analysis in plant-pathogen interactions. Here, we describe a protocol for the identification of pathogenicity and virulence genes through random insertional mutagenesis using the fungal wilt pathogen Verticillium dahliae as an example for the protocol. | 2012 | 22183674 |
| A bacterial artificial chromosome library for 'Jefferson' hazelnut and identification of clones associated with eastern filbert blight resistance and pollen-stigma incompatibility. | European hazelnut (Corylus avellana L.) is the only economically important nut crop in the family Betulaceae. Because of its small genome size (~385 Mb / 1C), relatively short life cycle, availability of a dense linkage map, and amenability to transformation by Agrobacterium, the European hazelnut could serve as a model plant for the Betulaceae. Here we report the construction of a bacterial artificial chromosome (BAC) library for 'Jefferson' hazelnut using the cloning enzyme MboI and the vector ... | 2011 | 21936690 |
| Improvement of cellulase activity in Trichoderma reesei by heterologous expression of a beta-glucosidase gene from Penicillium decumbens. | Trichoderma reesei is a well-known cellulase producer and widely applied in enzyme industry. To increase its ability to efficiently decompose cellulose, the beta-glucosidase activity of its enzyme cocktail needs to be enhanced. In this study, a beta-glucosidase I coding sequence from Penicillium decumbens was ligated with the cellobiohydrolase I (cbh1) promoter of T. reesei and introduced into the genome of T. reesei strain Rut-C30 by Agrobacterium-mediated transformation. In comparison to that ... | 2011 | 22112562 |
| Agrobacterium-mediated virus-induced gene silencing assay in cotton. | Cotton (Gossypium hirsutum) is one of the most important crops worldwide. Considerable efforts have been made on molecular breeding of new varieties. The large-scale gene functional analysis in cotton has been lagged behind most of the modern plant species, likely due to its large size of genome, gene duplication and polyploidy, long growth cycle and recalcitrance to genetic transformation(1). To facilitate high throughput functional genetic/genomic study in cotton, we attempt to develop rapid a ... | 2011 | 21876527 |
| Remediation of oil-based drill cuttings through a biosurfactant-based washing followed by a biodegradation treatment. | In this study, oil-based drill cuttings were washed by a rhamnolipid solution and then subjected to bioremediation in stainless steel boxes using sawdust as bulking agent. A mixed bacterial culture, mainly containing Pseudomonas, Acinetobacter, Alcaligenes, Agrobacterium, and Comamonas, was used as inoculums. Approximately 83% of organics were removed after washing under optimal conditions (liquid/solid ratio, 3:1; washing time, 20 min; stirring speed, 200 rpm; rhamnolipid concentration, 360 mg/ ... | 2011 | 21925873 |
| [6b genes: the important effective factors relative to tumor formation in plants]. | In recent years, the functional mechanisms of the oncogenens from Agrobacterium in plants were received more and more attentions. 6b genes, derived from the T-DNA fragment, are vital carcinogenesis factors of plants and belong to rolB genes family. In plants, 6b genes can affect phytohormone levels and carbohydrate contents, and can also cause accumulation of secondary metabolites, as well as change the relative genes expression. The specific mechanisms behind these impacts remain to be research ... | 2011 | 22120076 |
| genome sequence of agrobacterium tumefaciens strain f2, a bioflocculant-producing bacterium. | agrobacterium tumefaciens f2 is an efficient bioflocculant-producing bacterium. but the genes related to the metabolic pathway of bioflocculant biosynthesis in strain f2 are unknown. we present the draft genome of a. tumefaciens f2. it could provide further insight into the biosynthetic mechanism of polysaccharide-like bioflocculant in strain f2. | 2011 | 21914861 |
| Reduction of PDC1 expression in S. cerevisiae with xylose isomerase on xylose medium. | Ethanol production using hemicelluloses has recently become a focus of many researchers. In order to promote D: -xylose fermentation, we cloned the bacterial xylA gene encoding for xylose isomerase with 434 amino acid residues from Agrobacterium tumefaciens, and successfully expressed it in Saccharomyces cerevisiae, a non-xylose assimilating yeast. The recombinant strain S. cerevisiae W303-1A/pAGROXI successfully colonized a minimal medium containing D: -xylose as a sole carbon source and was ca ... | 2011 | 21989637 |
| Expression of a novel antimicrobial peptide Penaeidin4-1 in creeping bentgrass (Agrostis stolonifera L.) enhances plant fungal disease resistance. | Turfgrass species are agriculturally and economically important perennial crops. Turfgrass species are highly susceptible to a wide range of fungal pathogens. Dollar spot and brown patch, two important diseases caused by fungal pathogens Sclerotinia homoecarpa and Rhizoctonia solani, respectively, are among the most severe turfgrass diseases. Currently, turf fungal disease control mainly relies on fungicide treatments, which raises many concerns for human health and the environment. Antimicrobia ... | 2011 | 21931807 |
| Genetic transformation of Diaporthe phaseolorum, an endophytic fungus found in mangrove forests, mediated by Agrobacterium tumefaciens. | We describe the genetic transformation of the mycelial tissue of Diaporthe phaseolorum, an endophytic fungus isolated from the mangrove species Laguncularia racemosa, using Agrobacterium tumefaciens-mediated transformation (ATMT). ATMT uses both the hygromycin B resistant (hph) gene and green fluorescent protein as the selection agents. The T-DNA integration into the fungal genome was assessed by both PCR and Southern blotting. All transformants examined were mitotically stable. An analysis of t ... | 2011 | 22210192 |
| Overlapping protective roles for glutathione transferase gene family members in chemical and oxidative stress response in Agrobacterium tumefaciens. | In the present work, we describe the characterisation of the glutathione transferase (GST) gene family from Agrobacterium tumefaciens C58. A genome survey revealed the presence of eight GST-like proteins in A. tumefaciens (AtuGSTs). Comparison by multiple sequence alignment generated a dendrogram revealing the phylogenetic relationships of AtuGSTs-like proteins. The beta and theta classes identified in other bacterial species are represented by five members in A. tumefaciens C58. In addition, th ... | 2011 | 21909786 |
| genetic transformation of mature embryos of bread (t. aestivum) and pasta (t. durum) wheat genotypes. | the objective of the present study is to develop an efficient protocol for regeneration of transgenic wheat plants using agrobacterium- mediated transformation of mature embryos of hexaploid bread wheat (triticum aestivum) and tetraploid pasta wheat (triticum durum). the data indicated that embryogenic calli were formed within 7 days in the presence of 2 mgl-1 2,4-d. adventitious shoots emerged from the embryonic calli in the presence of 2 mgl-1 ba. shoot regeneration frequency varied between wh ... | 2010 | 21865876 |
| telomere-mediated truncation of barley chromosomes. | engineered minichromosomes offer an enormous opportunity to plant biotechnology as they have the potential to simultaneously transfer and stably express multiple genes. following a top-down approach, we truncated endogenous chromosomes in barley (hordeum vulgare) by agrobacterium-mediated transfer of t-dna constructs containing telomere sequences. blocks of arabidopsis-like telomeric repeats were inserted into a binary vector suitable for stable transformation. after transfer of these constructs ... | 2011 | 22080935 |
| construction of two vectors for gene expression in trichoderma reesei. | we report the construction of two filamentous fungi trichoderma reesei expression vectors, pwef31 and pwef32. both vectors possess the hygromycin phosphotransferase b gene expression cassette and the strong promoter and terminator of the cellobiohydrolase 1 gene (cbh1) from t. reesei. the two newly constructed vectors can be efficiently transformed into t. reesei with agrobacterium-mediated transformation. the difference between pwef31 and pwef32 is that pwef32 has two longer homologous arms. as ... | 2012 | 22056690 |
| extracellular virb5 enhances t-dna transfer from agrobacterium to the host plant. | virb5 is a type 4 secretion system protein of agrobacterium located on the surface of the bacterial cell. this localization pattern suggests a function for virb5 which is beyond its known role in biogenesis and/or stabilization of the t-pilus and which may involve early interactions between agrobacterium and the host cell. here, we identify virb5 as the first agrobacterium virulence protein that can enhance infectivity extracellularly. specifically, we show that elevating the amounts of the extr ... | 2011 | 22028781 |
| in vitro azadirachtin production by hairy root cultivation of azadirachta indica in nutrient mist bioreactor. | azadirachtin, a well-known biopesticide is a secondary metabolite conventionally extracted from the seeds of azadirachta indica. the present study involved in vitro azadirachtin production by developing hairy roots of a. indica via agrobacterium rhizogenes-mediated transformation of a. indica explants. liquid culture of hairy roots was established in shake flask to study the kinetics of growth and azadirachtin production. a biomass production of 13.3 g/l dry weight (specific growth rate of 0.7 d ... | 2011 | 22083394 |
| natural plant genetic engineer agrobacterium rhizogenes: role of t-dna in plant secondary metabolism. | agrobacterium rhizogenes is a natural plant genetic engineer. it is a gram-negative soil bacterium that induces hairy root formation. success has been obtained in exploring the molecular mechanisms of transferred dna (t-dna) transfer, interaction with host plant proteins, plant defense signaling and integration to plant genome for successful plant genetic transformation. t-dna and corresponding expression of rol genes alter morphology and plant host secondary metabolism. dur ... | 2011 | 22048847 |
| vanadium bromoperoxidase from delisea pulchra: enzyme-catalyzed formation of bromofuranone and attendant disruption of quorum sensing. | vanadium bromoperoxidase was isolated and cloned from the marine red alga delisea pulchra. this enzyme catalyzes the bromolactonization of 4-pentynoic acid forming 5e-bromo-methylidenetetrahydro-2-furanone, a compound which is shown herein to inhibit quorum sensing in the engineered reporter strain, agrobacterium tumefaciens ntl4. | 2011 | 22006105 |
| the pex7-mediated peroxisomal import system is required for fungal development and pathogenicity in magnaporthe oryzae. | in eukaryotes, microbodies called peroxisomes play important roles in cellular activities during the life cycle. previous studies indicate that peroxisomal functions are important for plant infection in many phytopathogenic fungi, but detailed relationships between fungal pathogenicity and peroxisomal function still remain unclear. here we report the importance of peroxisomal protein import through pts2 (peroxisomal targeting signal 2) in fungal development and pathogenicity of magnaporthe oryza ... | 2011 | 22194815 |
| effect of applying an arsenic-resistant and plant growth-promoting rhizobacterium to enhance soil arsenic phytoremediation by populus deltoides lh05-17. | bioremediation of highly arsenic (as)-contaminated soil is difficult because as is very toxic for plants and micro-organisms. the aim of this study was to investigate soil arsenic removal effects using poplar in combination with the inoculation of a plant growth-promoting rhizobacterium (pgpr). | 2011 | 21895895 |
| modified expression of alternative oxidase in transgenic tomato and petunia affects the level of tomato spotted wilt virus resistance. | abstract: | 2011 | 22014312 |
| stress-inducible expression of barley hva1 gene in transgenic mulberry displays enhanced tolerance against drought, salinity and cold stress. | coping with different kinds of biotic and abiotic stresses is the foundation of sustainable agriculture. although conventional breeding and marker-assisted selection are being employed in mulberry (morus indica l.) to develop better varieties, nonetheless the longer time periods required for these approaches necessitates the use of precise biotechnological approaches for sustainable agriculture. in an attempt to improve stress tolerance of mulberry, an important plant of the sericulture industry ... | 2011 | 22160463 |
| genetic transformation of colletotrichum truncatum associated with anthracnose disease of chili by random insertional mutagenesis. | an agrobacterium tumefaciens -mediated transformation (atmt) system was successfully developed for colletotrichum truncatum, the causal agent of chili anthracnose. a. tumefaciens carrying a hygromycin phosphotransferase gene (hph) and a green fluorescent protein (gfp) gene was used to transform the conidiospores of two c. truncatum pathotypes f8-3b and brip26974. optimum transformation efficiency was obtained when equal volumes of a. tumefaciens strain agl1 carrying either pjf1 or ppk2 binary ve ... | 2011 | 22052577 |
| efficient transformation and regeneration of fig (ficus carica l.) via somatic embryogenesis. | fig is one of the most important fruit trees in egypt. it used to constitute the major source of income for the inhabitants of the western north coast of egypt. since 1993 fig cultivations were threatened by a number of factors including virus, insect and mite infections. an efficient system for regeneration and transformation of the common fig ficus carica l. cultivar sultani (fresh consumption) was required to conserve fig cultivation in the area. the effect of different combinations of ba and ... | 2010 | 21912211 |
| selection of reference genes for qpcr in hairy root cultures of peanut. | abstract: | 2011 | 21985172 |
| zinc finger nuclease and homing endonuclease-mediated assembly of multigene plant transformation vectors. | binary vectors are an indispensable component of modern agrobacterium tumefaciens-mediated plant genetic transformation systems. a remarkable variety of binary plasmids have been developed to support the cloning and transfer of foreign genes into plant cells. the majority of these systems, however, are limited to the cloning and transfer of just a single gene of interest. thus, plant biologists and biotechnologists face a major obstacle when planning the introduction of multigene traits into tra ... | 2011 | 22082504 |
| the conjugation protein tcpc from clostridium perfringens is structurally related to the type iv secretion system protein virb8 from gram-negative bacteria. | bacterial conjugation is important for the acquisition of virulence and antibiotic resistance genes. we investigated the mechanism of conjugation in gram-positive pathogens using a model plasmid pcw3 from clostridium perfringens. pcw3 encodes tetracycline resistance and contains the tcp locus, which is essential for conjugation. we showed that the unique tcpc protein (359 amino acids, 41 kda) was required for efficient conjugative transfer, localized to the cell membrane independently of other c ... | 2011 | 22150951 |
| a point mutation in the medicago sativa gsa gene provides a novel, efficient, selectable marker for plant genetic engineering. | bacterial selectable marker genes (smg) conferring antibiotic resistance are valuable tools in plant genetic engineering, but public concern and regulatory requirements have stimulated the development of alternative selection systems. we have previously demonstrated that a mutated synechococcus elongatus heml gene encoding glutamate 1-semialdehyde aminotransferase (gsa) is an efficient smg in alfalfa. in fact, gsa is irreversibly inhibited by gabaculine (3-amino-2,3-dihydrobenzoic acid), but the ... | 2011 | 21875626 |
| high level expression of acidothermus cellulolyticus beta-1, 4-endoglucanase in transgenic rice enhances the hydrolysis of its straw by cultured cow gastric fluid. | abstract: background: large-scale production of effective cellulose hydrolytic enzymes is the key to bioconversion of agricultural residues to ethanol. the goal of this study was to develop rice plant as a bioreactor for large-scale production of cellulose hydrolytic enzymes via genetic transformation and to improve rice straw simultaneously as an efficient biomass feedstock for conversion of cellulose to glucose. results: in this study, the cellulose hydrolytic enzyme beta-1, 4-endoglucanase ( ... | 2011 | 22152050 |
| agrobacterium rhizogenes-mediated transformation of the parasitic plant phtheirospermum japonicum. | plants within the orobanchaceae are an agriculturally important group of parasites that attack economically important crops to obtain water and nutrients from their hosts. despite their agricultural importance, molecular mechanisms of the parasitism are poorly understood. | 2011 | 21991355 |
| the agrobacterium-mediated transformation of common wheat (triticum aestivum l.) and triticale (x triticosecale wittmack): role of the binary vector system and selection cassettes. | the influence of two binary vector systems, pgreen and pcambia, on the agrobacterium-mediated transformation ability of wheat and triticale was studied. both vectors carried selection cassettes with bar or nptii driven by different promoters. two cultivars of wheat, kontesa and torka, and one cultivar of triticale, wanad, were tested. the transformation rates for the wheat cultivars ranged from 0.00 to 3.58% and from 0.00 to 6.79% for triticale. the best values for wheat were 3.58% for kontesa a ... | 2011 | 21952729 |
| Flavonoid production in transgenic hop (Humulus lupulus L.) altered by PAP1/MYB75 from Arabidopsis thaliana L. | Hop is an important source of secondary metabolites, such as flavonoids. Some of these are pharmacologically active. Nevertheless, the concentration of some classes as flavonoids in wild-type plants is rather low. To enhance the production in hop, it would be interesting to modify the regulation of genes in the flavonoid biosynthetic pathway. For this purpose, the regulatory factor PAP1/AtMYB75 from Arabidopsis thaliana L. was introduced into hop plants cv. Tettnanger by Agrobacterium-mediated g ... | 2012 | 21912858 |
| Improving UV resistance and virulence of Beauveria bassiana by genetic engineering with an exogenous tyrosinase gene. | Insect pathogenic fungi like Beauveria bassiana have been developed as environmentally friendly biocontrol agents against arthropod pests. However, restrictive environmental factors, including solar ultraviolet (UV) radiation frequently lead to inconsistent field performance. To improve resistance to UV damage, we used Agrobacterium-mediated transformation to engineer B. bassiana with an exogenous tyrosinase gene. The results showed that the mitotically stable transformants produced larger amoun ... | 2012 | 22024554 |
| development of transgenic watermelon resistant to cucumber mosaic virus and watermelon mosaic virus by using a single chimeric transgene construct. | watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. to develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer dna from the partial n gene of watermelon silver mottle virus (wsmov) fused to the partial coat protein (cp) gene sequences of cucumber mosaic virus (cmv), cucumber green mottle mosaic virus (cgmmv) and watermelon mosaic virus (wmv) was construc ... | 2011 | 22203520 |
| solanum lycopersicum cytokinin response factor (slcrf) genes: characterization of crf domain-containing erf genes in tomato. | cytokinin is an influential hormone in growth and developmental processes across many plant species. while several cytokinin-regulated genes have been well characterized in arabidopsis, few have been identified in tomato, solanum lycopersicum. here a tomato family of 11 highly related cytokinin response factor genes designated as slcrf1-slcrf11 (solanum lycopersicum cytokinin response factor) are identified and characterized. slcrfs are ap2/erf transcription factors and generally orthologous to ... | 2011 | 22068146 |
| glucose and plant exudate enhanced enumeration of bacteria capable of degrading polycyclic aromatic hydrocarbons. | enumerating environmental microbial isolates capable of polycyclic aromatic hydrocarbon (pah) degradation can provide insight into the microbe-plant interactions that facilitate pah removal. we examined a known pah degrader ( pseudomonas putida g7), a nondegrader ( agrobacterium tumefaciens lba4404), and several microorganisms isolated from the environment by using a pah cocktail in an enumeration medium with or without 0.025% (m/v) glucose and (or) root exudates. compared with the standard m ... | 2011 | 22136124 |
| generation of transgenic rice lines with reduced contents of multiple potential allergens using a null mutant in combination with an rna silencing method. | rice seed proteins are known to be a causative antigen in some patients with food allergy, especially cereal allergy, with clinical symptoms such as eczema and dermatitis. the α-amylase/trypsin inhibitors (14-16 kda), α-globulin (26 kda) and β-glyoxalase i (33 kda) are regarded as major potential allergens of rice (oryza sativa l.) seed based on specific recognition by serum ige from allergy patients. in order to suppress the production of these major allergens in rice grains, a mutant in the 'k ... | 2011 | 22039121 |
| Transient silencing of the grapevine gene VvPGIP1 by agroinfiltration with a construct for RNA interference. | Grapevine is an economically important crop, and the recent completion of its genome makes it possible to study the function of specific genes through reverse genetics. However, the analysis of gene function by RNA interference (RNAi) in grapevine is difficult, because the generation of stable transgenic plants has low efficiency and is time consuming. Recently, transient expression of genes in grapevine leaves has been obtained by Agrobacterium tumefaciens infiltration (agroinfiltration). We th ... | 2012 | 21932028 |
| identification of pathogenesis associated genes by t-dna-mediated insertional mutagenesis in botrytis cinerea: a type 2a phosphoprotein phosphatase and a spt3 transcription factor have significant impact on virulence. | agrobacterium tumefaciens - mediated transformation (atmt) was used to generate an insertional mutant library of the grey mould fungus botrytis cinerea. from a total of 2,367 transformants, 68 mutants showing significant reduction in virulence on tomato and bean plants were analyzed in detail. as reported for other fungal atmt libraries, integrations were mostly single copy, occurred preferentially in non-coding (regulatory) regions, and were frequently accompanied by small deletions of the targ ... | 2011 | 22112214 |
| agrobacterium tumefaciens-mediated transformation of aspergillus aculeatus for insertional mutagenesis. | abstract: agrobacterium tumefaciens-mediated transformation (amt) was applied to aspergillus aculeatus. transformants carrying the t-dna from a binary vector pbig2rhph2 were sufficiently mitotically stable to allow functional genomic analyses. the amt technique was optimized by altering the concentration of acetosyringone, the ratio and concentration of a. tumefaciens and a. aculeatus cells, the duration of co-cultivation, and the status of a. aculeatus cells when using conidia, protoplasts, o ... | 2011 | 22166586 |
| morphological and physiological characteristics of transgenic cherry tomato mutant with hbsag gene. | hbsag gene was previously introduced into cherry tomato (lycopersicum esculentum var. cerasiforme) by agrobacterium-mediated transformation. to investigate the side effect of hbsag gene in cherry tomato, we analyzed morphological and physiological characteristics of the transgenic mutant n244. the process was performed under field conditions. the results suggested that the mutant n244 exhibited morphological, cytological and physiological variation. first of all, compared with the wild plants nk ... | 2011 | 21954613 |
| detection of acylated homoserine lactones produced by vibrio spp. and related species isolated from water and aquatic organisms. | aims: to assess the diversity in production of acylated homoserine lactones (ahls) among vibrio spp and related species. methods and results: a total of 106 isolates, with representatives of 28 vibrio spp and related species, were investigated for the production of ahls. for this, a rapid method for the screening of ahls was developed based on the use of bacterial biosensors using a double-layer microplate assay. at least one bacterial biosensor was activated in 20 species, agrobacterium tumef ... | 2011 | 22098208 |
| [discovery and research progress of endophytic bacteria in the root nodules of legumes--a review]. | recently, some research reports showed varied endophytic bacteria in the root nodules of some legumes, which attracts great interest in research field of rhizobia. here, we reviewed the discovery, identification of some endophytic bacteria (agrobacterium, non-symbiotic rhizobia, and other bacteria) in root nodules and their influence on symbiosis or plant growth, to understand the microecosystem of root nodule and to extend the field of rhizobia research. | 2011 | 22097764 |
| studying interactions between chloroplast proteins in intact plant cells using bimolecular fluorescence complementation and förster resonance energy transfer. | protein-protein interactions play crucial roles in the execution of many cellular functions, including those in plastids. identifying and characterising protein-protein interactions can yield valuable information regarding the function of a protein and can also contribute towards understanding protein-protein interaction networks in plastids, thereby contributing to a better understanding of cellular processes. here, we describe the planning and experimental procedures required to perform both b ... | 2011 | 21863438 |
| modulation of plumbagin production in plumbago zeylanica using a single-chain variable fragment antibody against plumbagin. | a single-chain variable fragment antibody (scfv) against plumbagin (pl) accumulated the pl production in the hairy roots of plumbago zeylanica. recombinant agrobacterium rhizogenes (atcc 15834) containing an scfv gene against pl (pl-scfv) were obtained through triparental mating and transformed into p. zeylanica to induce pl-scfv protein in the hairy roots. up to 40 μg recombinant pl-scfv were expressed per milligram of soluble protein in transgenic p. zeylanica hairy root cultures. the mean pl ... | 2012 | 21909936 |
| characterization of the mmsab-arad1 (gguabc) genes of agrobacterium tumefaciens. | the chve-gguabc operon plays a critical role in both virulence and sugar utilization through the activities of the periplasmic chve protein, which binds to a variety of sugars. the roles of the ggua, ggub, and gguc are not known. while ggua and ggub are homologous to bacterial abc transporters, earlier genetic analysis indicated that they were not necessary for utilization of sugars as the sole carbon source. to further examine this issue, in-frame deletions were constructed separately for each ... | 2011 | 21984786 |
| rapid in vivo analysis of synthetic promoters for plant pathogen phytosensing. | we aimed to engineer transgenic plants for the purpose of early detection of plant pathogen infection, which was accomplished by employing synthetic pathogen inducible promoters fused to reporter genes for altered phenotypes in response to the pathogen infection. toward this end, a number of synthetic promoters consisting of inducible regulatory elements fused to a red fluorescent protein (rfp) reporter were constructed for use in phytosensing. | 2011 | 22093754 |
| identification of seed dormancy mutants by activation tagging. | activation tagging is an important tool for gene discovery in plants. this method utilizes a t-dna sequence that contains four tandem copies of the cauliflower mosaic virus 35s enhancer sequence or promoters oriented outward to the t-dna border sequences. these elements enhance the expression of genes neighboring on either side of the randomly integrated t-dna, resulting in gain-of-function phenotypes. activation tagging has identified a number of genes, including those fundamental to plant deve ... | 2011 | 21898257 |
| novel cellulose-binding-domain protein in phytophthora is cell wall localized. | cellulose binding domains (cbd) in the carbohydrate binding module family 1 (cbm1) are structurally conserved regions generally linked to catalytic regions of cellulolytic enzymes. while widespread amongst saprophytic fungi that subsist on plant cell wall polysaccharides, they are absent amongst most plant pathogenic fungal cellulases. a genome wide survey for cbm1 was performed on the highly destructive plant pathogen phytophthora infestans, a fungal-like stramenopile, to determine if it harbor ... | 2011 | 21887271 |
| V2 protein encoded by Tomato yellow leaf curl China virus is an RNA silencing suppressor. | The V2 protein of Tomato yellow leaf curl China virus (TYLCCNV) was identified as an RNA silencing suppressor by Agrobacterium-mediated co-infiltration. The V2 protein could inhibit local RNA silencing, systemic RNA silencing of the green fluorescent protein (GFP) gene and the spread of a systemic GFP RNA silencing signal. However, the V2 could not interfere with the cell-to-cell spread of RNA silencing. Subcellular localization assay indicated that the V2 protein was distributed in the cytoplas ... | 2011 | 21893116 |
| expression of ceff significantly decreased deacetoxycephalosporin c formation during cephalosporin c production in acremonium chrysogenum. | deacetoxycephalosporin c (daoc) is not only the precursor but also one of the by-products during cephalosporin c (cpc) biosynthesis. one enzyme (daoc/dac synthase) is responsible for the two-step conversion of penicillin n into deacetylcephalosporin c (dac) in acremonium chrysogenum, while two enzymes (daoc synthase and daoc hydroxylase) were involved in this reaction in streptomyces clavuligerus and amycolatopsis lactamdurans (nocardia lactamdurans). in this study, the daoc hydroxylase gene cef ... | 2011 | 21866341 |
| Methyl jasmonate elicitation enhances glycyrrhizin production in Glycyrrhiza inflata hairy roots cultures. | Hairy roots were induced by infecting stems and leaves of Glycyrrhiza inflata with Agrobacterium rhizogenes ATCC 15834. The optimization of growth and glycyrrhizin accumulation of G. inflata hairy roots was studied. Sucrose (6%, w/v) was optimal for growth and glycyrrhizin accumulation in G. inflata hairy roots. Effects of elicitors like chitosan, methyl jasmonate, and yeast extract on glycyrrhizin production were studied. Methyl jasmonate (100 microM) was most efficient in enhancing glycyrrhizi ... | 2011 | 21950168 |
| a novel gateway®-compatible binary vector allows direct selection of recombinant clones in agrobacterium tumefaciens. | abstract: | 2011 | 22145613 |
| Biodegradation of crystal violet by Agrobacterium radiobacter. | Agrobacterium radiobacter MTCC 8161 completely decolorized the Crystal Violet with 8 hr (10 mg/L) at static anoxic conditions. The decreased decolorization capability by A. radiobacter was observed, when the Crystal Violet concentration was increased from 10 to 100 mg/L. Semi-synthetic medium containing 1% yeast extract and 0.1% NH4C1 has shown 100% decolorization of Crystal Violet within 5 hr. A complete degradation of Crystal Violet by A. radiobacter was observed up to 7 cycles of repeated add ... | 2011 | 22128547 |
| recent advances in curdlan biosynthesis, biotechnological production, and applications. | curdlan is a water-insoluble β-(1,3)-glucan produced by agrobacterium species under nitrogen-limited condition. its heat-induced gelling properties render curdlan to be very useful in the food industry initially. recent advances in the understanding of the role curdlan plays in both innate and adaptive immunity lead to its growing applications in biomedicine. our review focuses on the recent advances on curdlan biosynthesis and the improvements of curdlan fermentation production both from our la ... | 2011 | 22124723 |
| Enhancing the recombinant protein expression of halohydrin dehalogenase HheA in Escherichia coli by applying a codon optimization strategy. | Halohydrin dehalogenases are attractive biocatalysts in producing a series of important chiral building blocks. Recombinant expression of halohydrin dehalogenase from Arthrobacter sp. AD2 (HheA) in Escherichia coli using T7 promoter-based pGEF(+) system revealed much lower expression level than that of the well-studied halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC). In this study, we changed the codon usage in the 5'-end of hheA gene to improve the expression yield of HheA. Ou ... | 2011 | 22112566 |
| chlorimuron ethyl as a new selectable marker for disrupting genes in the insect-pathogenic fungus metarhizium robertsii. | a lack of selectable markers was a hindrance in investigating gene function in metarhizium robertsii. a reliable agrobacterium-mediated transformation system based on the use of chlorimuron ethyl as the selectable marker was developed which could serve as a useful tool to inactivate genes involved in insect pathogenicity. | 2011 | 21851837 |
| Molecular cloning and functional characterization of Catharanthus roseus hydroxymethylbutenyl 4-diphosphate synthase gene promoter from the methyl erythritol phosphate pathway. | The Madagascar periwinkle produces monoterpenoid indole alkaloids (MIA) of high interest due to their therapeutical values. The terpenoid moiety of MIA is derived from the methyl erythritol phosphate (MEP) and seco-iridoid pathways. These pathways are regarded as the limiting branch for MIA biosynthesis in C. roseus cell and tissue cultures. In previous studies, we demonstrated a coordinated regulation at the transcriptional and spatial levels of genes from both pathways. We report here on the i ... | 2011 | 22160472 |
| Phosphate-bound structure of an organophosphate-degrading enzyme from Agrobacterium radiobacter. | OpdA is a binuclear metalloenzyme that can hydrolyze organophosphate pesticides and nerve agents. In this study the crystal structure of the complex between OpdA and phosphate has been determined to 2.20Å resolution. The structure shows the phosphate bound in a tripodal mode to the metal ions whereby two of the oxygen atoms of PO(4) are terminally bound to each metal ion and a third oxygen bridges the two metal ions, thus displacing the µOH in the active site. In silico modelling demonstrates th ... | 2012 | 22112835 |
| arabidopsis los5/aba3 overexpression in transgenic tobacco (nicotiana tabacum cv. xanthi-nc) results in enhanced drought tolerance. | drought is a major environmental stress factor that affects growth and development of plants. abscisic acid (aba), osmotically active compounds, and synthesis of specific proteins, such as proteins that scavenge oxygen radicals, are crucial for plants to adapt to water deficit. los5/aba3 (los5) encodes molybdenum-cofactor sulfurase, which is a key regulator of aba biosynthesis. we overexpressed los5 in tobacco using agrobacterium-mediated transformation. detached leaves of los5-overexpressing se ... | 2011 | 21889046 |
| Biogeography of symbiotic and other endophytic bacteria isolated from medicinal Glycyrrhiza species in China. | A total of 159 endophytic bacteria were isolated from surface-sterilized root nodules of wild perennial Glycyrrhiza legumes growing on 40 sites in central and northwestern China. Amplified fragment length polymorphism (AFLP) genomic fingerprinting and sequencing of partial 16S rRNA genes revealed that the collection mainly consisted of Mesorhizobium, Rhizobium, Sinorhizobium, Agrobacterium and Paenibacillus species. Based on symbiotic properties with the legume hosts Glycyrrhiza uralensis and Gl ... | 2011 | 22066910 |
| agrobacterium-mediated transformation of eucalyptus globulus using explants with shoot apex with introduction of bacterial choline oxidase gene to enhance salt tolerance. | eucalyptus globulus is one of the most economically important plantation hardwoods for paper making. however, its low transformation frequency has prevented genetic engineering of this species with useful genes. we found the hypocotyl section with a shoot apex has the highest regeneration ability among another hypocotyl sections, and have developed an efficient agrobacterium-mediated transformation method using these materials. we then introduced a salt tolerance gene, namely a bacterial choline ... | 2012 | 22009051 |
| expression and large-scale production of the biochemically active human tissue-plasminogen activator in hairy roots of oriental melon (cucumis melo). | human tissue-plasminogen activator (t-pa) is a thrombolytic protein that plays an active role in dissolving fibrin clots by fibrinolysis and in activating plasminogen to plasmin in blood vessels. t-pa and synthetic t-pa (st-pa) genes were expressed as enzymatically active form in hairy roots of oriental melon (cucumis melo l. cv. geumssaragi-euncheon) infected by agrobacterium rhizogenes. the insertion of the t-pa genes in genomic dna of transgenic hairy roots was verified by pcr. the presence a ... | 2011 | 22018738 |
| testing the 3'-untranslated rna regions of beet necrotic yellow vein virus and beet yellows virus as inducers of posttranscriptional gene silencing. | | 2011 | 21928144 |
| Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue. | Small heat shock proteins are involved in stress tolerance. We previously isolated and characterized a rice cDNA clone, Oshsp26, encoding a chloroplast-localized small heat shock protein that is expressed following oxidative or heat stress. In this study, we transferred this gene to tall fescue plants by an Agrobacterium-mediated transformation system. The integration and expression of the transgene was confirmed by PCR, Southern, northern, and immunoblot analyzes. Compared to the control plants ... | 2011 | 21984008 |
| Screening & profiling of quorum sensing signal molecules in Pseudomonas aeruginosa isolates from catheterized urinary tract infection patients. | Catheter associated urinary tract infections are the second most common nosocomial infections and Pseudomonas aeruginosa is the third most common organism responsible for these infections. In this study P. aeruginosa isolates from catheterized urinary tract infection patients were screened and profiled for the presence of different type of quorum sensing (QS) signal molecules. | 2011 | 21911974 |
| Genetic transformation of Salvia austriaca by Agrobacterium rhizogenes and diterpenoid isolation. | Hairy roots of Salvia austriaca Jacq. transformed with Agrobacterium rhizogenes strain A4 were obtained and transgenic status of the roots was confirmed by polymerase chain reaction (PCR) using rolB and rolC specific primers. The root cultures growing in half-strength Gamborg (1/2 B5) liquid medium supplemented with sucrose (30 g L(-1)) under light conditions (photoperiod: 16 h light/8 h dark) were examined for their ability to produce diterpenoids. From n-hexane extract the abietane-type diterp ... | 2011 | 22204139 |
| Improved Agrobacterium-mediated transformation of cowpea via sonication and vacuum infiltration. | An improved method of Agrobacterium-mediated transformation of cowpea was developed employing both sonication and vacuum infiltration treatments. 4 day-old cotyledonary nodes were used as explants for co-cultivation with Agrobacterium tumefaciens strain EHA105 harbouring the binary vector pSouv-cry1Ac. Among the different injury treatments, vacuum infiltration and their combination treatments tested, sonication for 20 s followed by vacuum infiltration for 5 min with A. tumefaciens resulted in hi ... | 2011 | 21853337 |
| Overexpression of a cotton cyclophilin gene (GhCyp1) in transgenic tobacco plants confers dual tolerance to salt stress and Pseudomonas syringae pv. tabaci infection. | The full-length cDNA of a cyclophilin-like gene was cloned from Gossypium hirsutum using rapid amplification of cDNA ends and was designated as GhCyp1, a member of the immunophilin protein family. GhCyp1 expression level was higher in roots and stems than in other tissues of cotton, as determined by real-time reverse transcription polymerase chain reaction (RT-PCR). To characterize the GhCyp1 gene, tobacco (Nicotiana tabacum) was transformed via Agrobacterium tumefaciens with a vector to express ... | 2011 | 22000049 |
| resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in cell cultures of vitis amurensis treated with coumaric acid. | the present study examines the effect of p-coumaric acid (ca), a precursor of stilbenes and isoflavonoids, on biosynthesis of resveratrol in cell cultures of vitis amurensis. earlier, we transformed v. amurensis with the rolb gene of agrobacterium rhizogenes and showed increased level of resveratrol production in the rolb transgenic cell culture. we used control and the rolb-transgenic cell culture of v. amurensis as a model system in this study. ca was capable of increasing resveratrol producti ... | 2011 | 21938424 |
| an efficient agrobacterium-mediated transient transformation of arabidopsis. | agrobacterium tumefaciens-mediated transient transformation has been a useful procedure for characterization of proteins and their functions in plants, including analysis of protein-protein interactions. agrobacterium-mediated transient transformation of nicotiana benthamiana by leaf infiltration has been widely used due to its ease and high efficiency. however, in arabidopsis this procedure has been challenging. previous studies suggested that this difficulty was caused by plant immune response ... | 2011 | 22004025 |
| [isolation and characterization of promoter of ads from artemisia annua]. | to try to find the ways to enhance the expression of ads gene encoding amorpha-4,11-diene synthase, a key enzyme in artemisinin biosynthesis pathway catalyzing the formation of amorpha-4,11-diene from farnesyl diphosphate, and accelerate the artemisinin synthesis, the promoter of ads was isolated and characterized. | 2011 | 22066438 |
| evolution of vacuolar proton pyrophosphatase domains and volutin granules: clues into the early evolutionary origin of the acidocalcisome. | volutin granules appear to be universally distributed and are morphologically and chemically identical to acidocalcisomes, which are electron-dense granular organelles rich in calcium and phosphate, whose functions include storage of phosphorus and various metal ions, metabolism of polyphosphate, maintenance of intracellular ph, osmoregulation and calcium homeostasis. prokaryotes are thought to differ from eukaryotes in that they lack membrane-bounded organelles. however, it has been demonstrate ... | 2011 | 21974828 |
| bt brinjal in india: a long way to go. | brinjal occupies the major proportion amongst all vegetable crops in india and is vulnerable to many diseases caused by insect-pests, fungus, bacteria and virus. brinjal production is extensively affected by the insect brinjal fruit and shoot borer. use of conventional chemical pesticides not only damage environment including the biotic and abiotic components but, also affect human health. bt brinjal was developed to combat brinjal fruit and shoot borer that has an advantage minimizing use of ch ... | 2011 | 21865863 |
| functional characterization of sugarcane myb transcription factor gene promoter (pscmybas1) in response to abiotic stresses and hormones. | the sugarcane (saccharum officinarum) stress-related myb transcription factor gene, scmybas1, demonstrated induced response to water deficit and salt stress in our previous study. to elucidate its stress tolerance mechanism at the transcriptional level, we isolated and characterized the promoter (pscmybas1, 1,033 bp) flanking the 5' scmybas1 coding region from the sugarcane genome. a series of pscmybas1 deletion derivatives from the transcription start site (-56, -152, -303, -442, -613, -777, -8 ... | 2011 | 22083650 |
| virus-derived ssdna vectors for the expression of foreign proteins in plants. | plant viruses with ssrna genomes provide a unique opportunity for generating expression vehicles for biopharming in plants, as constructs containing only the replication origin, with the replication-associated protein (rep) gene provided in cis or in trans, can be replicationally amplified in vivo by several orders of magnitude, with significant accompanying increases in transcription and expression of gene(s) of interest. appropriate replicating vectors or replicons may be derived from several ... | 2011 | 22038412 |
| enhancing transgenic pea (pisum sativum l.) resistance against fungal diseases through stacking of two antifungal genes (chitinase and glucanase). | one way of enhancing and broadening resistance of plants to different biotic and abiotic stresses is to combine transgenes expressing several genes into a single line. this can be done using different strategies such as crossing, single vector with multiple genes, co-transformation, sequential transformation and ires elements. in the present study conventional crossing method was used. parental transgenic lines transformed via agrobacterium tumefasciens-mediated gene transformation with pgreenii ... | 2011 | 21971070 |
| production of camptothecin by hairy roots and regenerated transformed shoots of ophiorrhiza rugosa var. decumbens. | camptothecin (cpt), the derivatives of which are used clinically for the treatment of metastatic colon cancer, is isolated from intact plants that can be subjected to environmental fluctuations. in vitro cultures may be an alternate and continuous source for year-round production of cpt. since cpt production by undifferentiated cell cultures is low, differentiated tissues such as root cultures may be a viable alternate source for cpt production. hairy roots were induced in ophiorriza rugosa, a s ... | 2011 | 21899480 |
| [construction of a bivalent plant expression vector carrying vvsuc11 and vvsuc12 genes and its genetic transformation in sugar beet]. | we have recombined genes vvsuc11, vvsuc12 from vitis vinifera l., and root-specific promoters of sweet potato storage protein gene from ipomoea batatas l. lam., named as sp1 and sp2. we have constructed a vector pcambia2301-sp1- vvsuc11-sp2-vvsuc12 using pcambia2301 as an original vector. vvsuc11 and vvsuc12 were under the control of root-specific promoters of sweet potato storage protein gene. we transformed the vector into kws-9103 breeding line of beta vulgaris l. with agrobacterium-mediated ... | 2011 | 22097805 |
| gateway-compatible vectors for high-throughput gene functional analysis in switchgrass (panicum virgatum l.) and other monocot species. | switchgrass (panicum virgatum l.) is a c4 perennial grass and has been identified as a potential bioenergy crop for cellulosic ethanol because of its rapid growth rate, nutrient use efficiency and widespread distribution throughout north america. the improvement of bioenergy feedstocks is needed to make cellulosic ethanol economically feasible, and genetic engineering of switchgrass is a promising approach towards this goal. a crucial component of creating transgenic switchgrass is having the ca ... | 2011 | 21955653 |
| transgenic rice plants harboring the grain hardness-locus region of aegilops tauschii. | grain hardness of wheat is determined by the hardness (ha)-locus region, which contains three friabilin-related genes: puroindoline-a (pina), puroindoline-b (pinb) and gsp-1. in our previous study, we produced the transgenic rice plants harboring the large genomic fragment of the ha-locus region of aegilops tauschii containing pina and gsp-1 genes by agrobacterium-mediated transformation. to examine the effects of the transgenes in the rice endosperms, we firstly confirmed the homozygosity of th ... | 2011 | 21850595 |
| [Regeneration of transgenic plants from Cichorium intybus L. var. foliosum Hegi hairy roots]. | Transgenic plants were regenerated from Cichorium intybus L. hairy roots transformed with genes of tuberculosis antigenes ESAT6 and Ag85B or human interferon alpha2b. The plant regeneration was light-dependent and occurred on the media without growth regulators. The DNA PCR and RT-PCR analyses have shown the presence and expression both selective and target genes in all root lines and regenerated plants. | 2011 | 22168044 |
| Agrobacterium rhizogenes-dependent production of transformed roots from foliar explants of pepper (Capsicum annuum): a new and efficient tool for functional analysis of genes. | Pepper is known to be a recalcitrant species to genetic transformation via Agrobacterium tumefaciens. A. rhizogenes-mediated transformation offers an alternative and rapid possibility to study gene functions in roots. In our study, we developed a new and efficient system for A. rhizogenes transformation of the cultivated species Capsicum annuum. Hypocotyls and foliar organs (true leaves and cotyledons) of Yolo Wonder (YW) and Criollo de Morelos 334 (CM334) pepper cultivars were inoculated with t ... | 2011 | 22016085 |
| biotransformation studies using hairy root cultures - a review. | agrobacterium rhizogenes induced hairy root cultures are entering into a new juncture of functional research in generating pharmaceutical lead compounds by bringing about chemical transformations aided through its inherent enzyme resources. rational utilization of hairy root cultures as highly effective biotransformation systems has come into existence in the last twenty years involving a wide range of plant systems as well as exogenous substrates and diverse chemical reactions. to date, hairy r ... | 2011 | 21871554 |
| Development of an efficient gene-targeting system in Aspergillus luchuensis by deletion of the non-homologous end joining system. | The industrial fungus Aspergillus luchuensis is used to produce a distilled spirit in Okinawa Island, Japan. Recently, the genome sequence of A. luchuensis RIB2604 (Aspergillus awamori NBRC 4314) was revealed and many functional genes are now expected to be analyzed. Gene targeting is necessary for analyzing the function of a gene; however, gene targeting frequencies in A. luchuensis are very low. To develop a highly efficient gene-targeting system for A. luchuensis, we disrupted A. luchuensis l ... | 2011 | 21903470 |
| a high throughput barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots. | barley stripe mosaic virus (bsmv) is a single-stranded rna virus with three genome components designated alpha, beta, and gamma. bsmv vectors have previously been shown to be efficient virus induced gene silencing (vigs) vehicles in barley and wheat and have provided important information about host genes functioning during pathogenesis as well as various aspects of genes functioning in development. to permit more effective use of bsmv vigs for functional genomics experiments, we have developed ... | 2011 | 22031834 |
| [construction of expression vector driven by tobacco root-specific promoter and transformation into rhodiola hairy root]. | to construct root-specific promoter of plant expression vector and transformate into rhodiola hairy root. | 2011 | 22017001 |
| brucella abortus efp gene is required for an efficient internalization in hela cells. | numerous chromosomal virulence genes (chv) have been shown to play an important role in the ability of agrobacterium tumefaciens to transform plants. the a. tumefaciens chvh gene encodes a protein similar in sequence to the escherichia coli elongation factor p (ef-p). in a. tumefaciens this factor is required for tumor formation and for full expression of the vir genes, exerting its activity at a post-transcriptional level. cross-complementation assays suggest that the chvh gene and the efp gene ... | 2012 | 21983596 |
| Inhibition and dispersal of Agrobacterium tumefaciens biofilms by a small diffusible Pseudomonas aeruginosa exoproduct(s). | Environmental biofilms often contain mixed populations of different species. In these dense communities, competition between biofilm residents for limited nutrients such as iron can be fierce, leading to the evolution of competitive factors that affect the ability of competitors to grow or form biofilms. We have discovered a compound(s) present in the conditioned culture fluids of Pseudomonas aeruginosa that disperses and inhibits the formation of biofilms produced by the facultative plant patho ... | 2011 | 22105093 |
| cloning a peanut resveratrol synthase gene and its expression in purple sweet potato. | a resveratrol synthase gene was cloned from the peanut plant (arachis hypogaea) by rt-pcr and was transformed into purple sweet potato (ipomoea batatas) by agrobacterium-mediated transformation. stem sections were infected with bacterial solution of od(600) = 0.4 for 20 min and then cocultured for 2 days. infected explants were cultured on ms media containing 50 mg/l kanamycin, 0.02 mg/l naa and 1 mg/l 6-ba for bud induction or containing 75 mg/l kanamycin, 1.0 mg/l naa and 0.1 mg/l 6-ba for roo ... | 2012 | 21932029 |
| Transient co-expression of post-transcriptional gene silencing suppressors for increased in planta expression of a recombinant anthrax receptor fusion protein. | Potential epidemics of infectious diseases and the constant threat of bioterrorism demand rapid, scalable, and cost-efficient manufacturing of therapeutic proteins. Molecular farming of tobacco plants provides an alternative for the recombinant production of therapeutics. We have developed a transient production platform that uses Agrobacterium infiltration of Nicotiana benthamiana plants to express a novel anthrax receptor decoy protein (immunoadhesin), CMG2-Fc. This chimeric fusion protein, de ... | 2011 | 21954339 |
| Establishment of regeneration and transformation system of sugarcane cultivar GT54-9 (C9). | Plant regeneration protocols for sugarcane GT54-9(C9) cultivar were developed for direct organogenesis and indirect somatic embryogenesis, using young leaf segments as explants by studying the influence of different concentrations and types of cytokinin and auxin hormones. For the callus formation from young leaves, a medium containing 4mg/l 2,4-D was found very effective. For embryo formation, MS medium supplemented with 1mg/l Kin and 0.5 mg/l 2,4-D was used. While in the case of direct organog ... | 2011 | 21912218 |
| regeneration of multiple shoots from transgenic potato events facilitates the recovery of phenotypically normal lines: assessing a cry9aa2 gene conferring insect resistance. | the recovery of high performing transgenic lines in clonal crops is limited by the occurrence of somaclonal variation during the tissue culture phase of transformation. this is usually circumvented by developing large populations of transgenic lines, each derived from the first shoot to regenerate from each transformation event. this study investigates a new strategy of assessing multiple shoots independently regenerated from different transformed cell colonies of potato (solanum tuberosum l.). | 2011 | 21995716 |
| compositional equivalence of insect-protected glyphosate-tolerant soybean mon 87701 × mon 89788 to conventional soybean extends across different world regions and multiple growing seasons. | the soybean product mon 87701 × mon 89788 expresses both the cry1ac gene derived from bacillus thuringiensis and the cp4 epsps (5-enolpyruvylshikimate-3-phosphate synthase) gene derived from agrobacterium sp. strain cp4. each biotechnology-derived trait confers specific benefits of insect resistance and glyphosate tolerance, respectively. the purpose of this study was to compare the composition of seed and forage from this combined-trait product to those of conventional soybean grown in geog ... | 2011 | 21985102 |
| Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study. | Approaches to simplify and streamline the construction of full-length infectious cDNA clones (FL-cDNAs) are needed. Among desirable improvements are the ability to use total nucleic acids (TNA) extracts from infected hosts (to bypass viral purification limitations) for the direct one-step amplification of large FL-cDNAs, the possibility to inoculate plants with uncloned FL-cDNAs and the simplified cloning of these large molecules. | 2011 | 22040379 |
| Developmental and environmental regulatory pathways in alpha-proteobacteria. | Spatial and temporal control of cell differentiation and morphogenesis plays a key role in prokaryotes as well as eukaryotes. This is particularly important for bacteria that divide asymmetrically, as they generate two morphologically and functionally distinct daughter cells. Several alpha-proteobacteria, including the aquatic, free-living Caulobacter crescentus, the symbiotic rhizobia and the plant and animal pathogens Agrobacterium and Brucella, have been shown to undergo asymmetrical division ... | 2012 | 22201830 |
| Phylogenomics of Reichenowia parasitica, an Alphaproteobacterial Endosymbiont of the Freshwater Leech Placobdella parasitica. | Although several commensal alphaproteobacteria form close relationships with plant hosts where they aid in (e.g.,) nitrogen fixation and nodulation, only a few inhabit animal hosts. Among these, Reichenowia picta, R. ornata and R. parasitica, are currently the only known mutualistic, alphaproteobacterial endosymbionts to inhabit leeches. These bacteria are harbored in the epithelial cells of the mycetomal structures of their freshwater leech hosts, Placobdella spp., and these structures have no ... | 2011 | 22132238 |
| establishment of regeneration and transformation system in egyptian sesame (sesamum indicum l.) cv. sohag. | sesame (sesamum indicum l.) is an important oil crop in many tropical and sub-tropical regions of the world, yet has received little attention in applying modern biotechnology in its improvement due to regeneration and transformation difficulties. here within, we report the successful production of transgenic fertile plants of sesame (cv. sohag 1), after screening several cultivars. agrobacterium tumefaciens- carrying the pbi121 plasmid {neomycin phosphotransferase gene (nptii) and a β-glucuroni ... | 2011 | 22179191 |