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arsenic accumulation in a freshwater fish living in a contaminated river of corsica, france.the impact of arsenic (as) discharge related to a past mining activity in the bravona river and its tributary, the presa river, was investigated in an edible fish, the brown trout (salmo trutta). fish was sampled in four stations along a contamination gradient. arsenic was measured in five organs type (operculum, gills, liver, muscle, and axial skeleton). almost 70 years after the suspension of the mining activity, as levels in water remain high. the results indicated a strong as contamination i ...200919345997
microhabitat selection of discocotyle sagittata (monogenea: polyopisthocotylea) in farmed rainbow trout.microhabitat preference of the monogenean discocotyle sagittata (leuckart, 1842) was determined in late spring and late autumn in rainbow trout, oncorhynchus mykiss (walbaum), reared in the isle of man, u.k. discocotyle sagittata exhibits a preference for attachment to anterior gill arches: 29% of all worms occurred on gill arch i, 28% on ii, 25% on iii and 18% on iv. this distribution pattern on the introduced salmonid species is the same as reported for its native european host, the brown trou ...200819175205
ultrastructure of microtriches on the scolex of cyathocephalus truncatus (cestoda: spathebothriidea).the scolex surface of the mature spathebothriidean cyathocephalus truncatus (pallas, 1781), a parasite of the brown trout salmo trutta fario l., was studied using scanning and transmission electron microscopy. a particular attention was paid to microtriches, unique structure on the surface of the cestoda. the scolex of c. truncatus is covered with two types of filiform microtriches (filitriches): aciculate (approximately 3 microm long) and capillate (approximately 10 microm long). capillate micr ...200819175209
role of genetic refuges in the restoration of native gene pools of brown trout.captive-bred animals derived from native, alien, or hybrid stocks are often released in large numbers in natural settings with the intention of augmenting harvests. in brown trout (salmo trutta), stocking with hatchery-reared non-native fish has been the main management strategy used to maintain or improve depleted wild brown trout populations in iberian and other mediterranean regions. this measure has become a serious threat to the conservation of native genetic diversity, mainly due to introg ...200919183212
[contribution to comparative ophthalmo-morphology: the eye of the brown trout].the aim of this study was to assess similarities and differences between the trout eye and the human eye.200919384794
sixty years of anthropogenic pressure: a spatio-temporal genetic analysis of brown trout populations subject to stocking and population declines.analyses of historical samples can provide invaluable information on changes to the genetic composition of natural populations resulting from human activities. here, we analyse 21 microsatellite loci in historical (archived scales from 1927 to 1956) and contemporary samples of brown trout (salmo trutta) from six neighbouring rivers in denmark, to compare the genetic structure of wild populations before and after population declines and stocking with nonlocal strains of hatchery trout. we show th ...200919457206
susceptibility of selected freshwater fish species to a uk lactococcus garvieae isolate.gram-positive cocci recovered from diseased rainbow trout from a farm in england were characterized by different methods, including pulsed field gel electrophoresis, as virulent lactococcus garvieae serogroup 2 (pulsotype a1). groups of rainbow trout were kept at a range of temperatures and injected intraperitoneally (i.p.) with one of the uk isolates, l. garvieae 00021. the 18 degrees c and 16 degrees c groups showed 67% and 28% mortality, respectively, by day 27 post-injection. fish kept at 14 ...200919500233
ligand-specific induction of endocytosis in taste receptor cells.we demonstrate a ligand-specific induction of endocytosis in cells of juvenile brown trout taste buds. the process is fast, massive and selective, as only a few cells in each taste buds are stained by exposure of the oral cavity to the taste stimulant l-cysteine together with a dye at 20 degrees c. low temperature (+2 degrees c) and disruption of microtubules with nocodazole caused a substantial reduction in the number of taste cells stained, indicating endocytic uptake of dye and transport towa ...200919088209
weighing the evidence of ecological risk from pahs contamination in the estuarine environment of salina cruz bay, méxico.results of bulk-phase chemical measurements, toxicological tests combined with bioaccumulation measures in fishes, were used to evaluate the toxicity of the 16 usepa priority polycyclic aromatic hydrocarbons from the sediment collected from eight stations of the ventosa estuarine system, located close to the main center of processing oil in the mexican pacific coast. levels of the sum of polycyclic aromatic hydrocarbons varied from 22 to 6,850 microg kg(-1) dry weight. based on sediment quality ...201019266302
influence of hydrologic attributes on brown trout recruitment in low-latitude range margins.factors controlling brown trout salmo trutta recruitment in mediterranean areas are largely unknown, despite the relevance this may have for fisheries management. the effect of hydrological variability on survival of young brown trout was studied during seven consecutive years in five resident populations from the southern range of the species distribution. recruit density at the end of summer varied markedly among year-classes and rivers during the study period. previous work showed that egg de ...200919290547
prevalence of serum antibodies against saprolegnia parasitica in wild and farmed brown trout salmo trutta.the prevalence of serum antibodies against saprolegnia parasitica in wild and farmed brown trout salmo trutta from the province of le6n (nw spain) was studied by enzyme-linked immunosorbent assay (elisa). blood samples from healthy and saprolegnia-infected brown trout were collected over 2 yr with a seasonal periodicity (january, april, july and october) from a hatchery and river with frequent presence of saprolegniosis (river porma) and from a river in which the disease was rarely observed (riv ...200919301632
speciation of lead, copper, zinc and antimony in water draining a shooting range--time dependant metal accumulation and biomarker responses in brown trout (salmo trutta l.).the speciation of pb, cu, zn and sb in a shooting range run-off stream were studied during a period of 23 days. in addition, metal accumulation in gills and liver, red blood cell ala-d activity, hepatic metallothionine (cd/zn-mt) and oxidative stress index (gssg/ tgsh levels) in brown trout (salmo trutta l.) exposed to the stream were investigated. fish, contained in cages, were exposed and sampled after 0, 2, 4, 7, 9, 11 and 23 days of exposure. trace metals in the water were fractionated in si ...200919339035
epizootiology of myxobolus cerebralis, the causative agent of salmonid whirling disease in the rock creek drainage of west-central montana: 2004-2008.whirling disease, caused by the myxozoan parasite myxobolus cerebralis , remains a serious health threat to salmonid fish in the western united states. a previously published study on the epizootiology of whirling disease in the rock creek watershed of west-central montana, conducted from 1998 to 2003, showed that the intensity of m. cerebralis infections in sentinel trout increased significantly throughout the drainage and that the range of m. cerebralis had expanded considerably. in addition, ...201019891515
chromosomal study of native and hatchery trouts from italy (salmo trutta complex, salmonidae): conventional and fish analysis.a cytogenetic analysis was carried out using conventional staining, banding techniques and fluorescence in situ hybridization (fish) in italian populations of brown trout (salmo truttacomplex). all individuals analysed, belonging to the atlantic (at), marmoratus (ma), adriatic (ad) and mediterranean (me) lineages, showed remarkable karyotype uniformity, with diploid complement of 2n = 80 chromosomes, arm number (nf) of 102 and invariable karyotype composition. such uniformity was also observed w ...200919372669
liver histopathology in brown trout (salmo trutta f. fario) from the tinhela river, subjected to mine drainage from the abandoned jales mine (portugal).the objective of this study was to compare the occurrence of toxicopathic liver lesions in brown trout (salmo trutta fario) from tinhela river near the jales mine, both before implementation (2002) and after completion of the governmental mitigation program (2006). fish were caught in april 2002 and may 2006, using an electrofishing system at four sites: s0, reference station; s1, s2 and s3 as contaminated stations. in 2002, the hepatosomatic index (hsi) was significantly higher for trout captur ...200919390757
surface morphology of nicolla skrjabini (trematoda: opecoelidae), a common parasite of european freshwater fishes, as revealed by sem.scanning electron examination of trematode specimens belonging to nicolla skrjabini (iwanitzky 1928), collected from brown trout salmo trutta fario l. in the czech republic, made it possible to study their surface morphology including details not described previously. the tegument bears numerous characteristic papillae mainly around and inside the suckers and around the genital pore and minute sensory organs near the anterior body end. surface morphology may prove to be useful in distinguishing ...200919449029
tales from scales: old dna yields insights into contemporary evolutionary processes affecting fishes.salmon and trout populations are suffering declines in abundance and diversity over much of their range around the atlantic and pacific rims as a consequence of many factors. one method of dealing with the decline has been to produce them in hatcheries but the wisdom of this approach has been hotly debated (e.g. hilborn & winton 1993; waples 1999; brannon et al. 2004). one concern is that domesticated hatchery strains will interbreed with locally adapted wild fish; but how do we study the geneti ...200919457205
mercury cycling in stream ecosystems. 3. trophic dynamics and methylmercury bioaccumulation.trophic dynamics (community composition and feeding relationships) have been identified as important drivers of methylmercury (mehg) bioaccumulation in lakes, reservoirs, and marine ecosystems. the relative importance of trophic dynamics and geochemical controls on mehg bioaccumulation in streams, however, remains poorly characterized. mehg bioaccumulation was evaluated in eight stream ecosystems across the united states (oregon, wisconsin, and florida) spanning large ranges in climate, landscap ...200919475942
admixture analysis of stocked brown trout populations using mapped microsatellite dna markers: indigenous trout persist in introgressed populations.admixture between wild and captive populations is an increasing concern in conservation biology. understanding the extent of admixture and the processes involved requires identification of admixed and non-admixed individuals. this can be achieved by statistical methods employing bayesian clustering, but resolution is low if genetic differentiation is weak. here, we analyse stocked brown trout populations represented by historical (1943-1956) and contemporary (2000s) samples, where genetic differ ...200919515653
effect of lactococcus lactis clfp 100 and leuconostoc mesenteroides clfp 196 on aeromonas salmonicida infection in brown trout (salmo trutta).aeromonas salmonicida is the etiological agent of furunculosis in salmonid fish. this pathogen is important from an epizootic perspective because fish surviving an outbreak can remain lifelong asymptomatic carriers, serving as reservoirs of infection. as a result, the early detection and the control of infection are essential to prevent the spread of new furunculosis outbreaks. we have thus analyzed the effect of probiotic administration on the incidence of a. salmonicida in brown trout (salmo t ...200919556745
comparison of arsenic and antimony contents in tissues and organs of brown trout caught from the river presa polluted by ancient mining practices and from the river bravona in corsica (france): a survey study.the present study evaluates the concentrations of arsenic (as) and antimony (sb) in the intestine, liver, muscle, gonads, gills, and kidney of salmo trutta subsp. from the presa river in corsica (france; n = 10), which crosses an abandoned arsenic mine, and from the bravona river (reference site; n = 10). both metalloids were analyzed by means of icp-ms. the relationships between fish size (length and weight) and metalloid concentrations in their tissues were investigated by linear regression an ...200919253009
assessing maternal effects on metabolic rate dynamics along early development in brown trout (salmo trutta): an individual-based approach.routine metabolic rate (oxygen consumption) of individual eggs and larvae of brown trout (salmo trutta) originating from different families were monitored from fertilisation to the onset of emergence by means of flow through micro-respirometry. this measuring system revealed an accurate tool to measure oxygen consumption on small organisms at the individual level, and daily consumption proved to be very stable. the mass-specific metabolic rate remained low from fertilisation to hatching, and the ...201019568756
comparison of the susceptibility of brown trout (salmo trutta) and four rainbow trout (oncorhynchus mykiss) strains to the myxozoan tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (pkd).differences in susceptibility to the myxozoan parasite tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease (pkd), between four strains of rainbow trout (oncorhynchus mykiss) and brown trout (salmo trutta) were evaluated. fish were exposed to water enzootic for the parasite in the field for 5 days and were subsequently transferred to the laboratory. relative parasite load was determined after 2, 3 and 4 weeks post-exposure (wpe) by quantitative real-time pcr (qpcr) ...200919683396
role of introduction history and landscape in the range expansion of brown trout (salmo trutta l.) in the kerguelen islands.human-mediated biological invasions constitute interesting case studies to understand evolutionary processes, including the role of founder effects. population expansion of newly introduced species can be highly dependant on barriers caused by landscape features, but identifying these barriers and their impact on genetic structure is a relatively recent concern in population genetics and ecology. salmonid populations of the kerguelen islands archipelago are a favorable model system to address th ...201020133353
the olfactory organ of the trout salmo trutta fario: a novel localization for a progestin receptor.a progestin receptor (pr) has been detected in the olfactory organ of the trout salmo trutta fario. the specificity of this receptor was high for 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-dp), but it also bound 17alpha-hydroxy-progesterone (17alpha-ohp) and 21-hydroxyprogesterone (21-ohp), even when present at low concentrations (10-fold in relative binding affinity assay). progesterone (p) competed effectively at much higher concentrations (1,000-fold in relative binding affinity ...201019725068
seawater tolerance in atlantic salmon, salmo salar l., brown trout, salmo trutta l., and s. salar × s. trutta hybrids smolt.high levels of hybridization between atlantic salmon (salmo salar) and brown trout (salmo trutta) have been reported in the gyrodactylus salaris infected rivers vefsna and driva in norway. the survival and behaviour during the sea phase of such hybrids is unknown. the reported work documents ionoregulatory status after 24 h seawater challenge tests (24hsw) and gill na+/k+-atpase (nka) activity of migrating wild smolts of atlantic salmon, brown trout and hybrids at two sampling dates during the 2 ...201019821045
measurement of total zn and zn isotope ratios by quadrupole icp-ms for evaluation of zn uptake in gills of brown trout (salmo trutta) and rainbow trout (oncorhynchus mykiss).this study evaluates the potential use of stable zinc isotopes in toxicity studies measuring zinc uptake by the gills of brown trout (salmo trutta) and rainbow trout (oncorhynchus mykiss). the use of stable isotopes in such studies has several advantages over the use of radioisotopes, including cost, ease of handling, elimination of permit requirements, and waste disposal. a pilot study using brown trout was performed to evaluate sample preparation methods and the ability of a quadrupole inducti ...200919836537
evidence for small scale variation in the vertebrate brain: mating strategy and sex affect brain size and structure in wild brown trout (salmo trutta).the basis for our knowledge of brain evolution in vertebrates rests heavily on empirical evidence from comparative studies at the species level. however, little is still known about the natural levels of variation and the evolutionary causes of differences in brain size and brain structure within-species, even though selection at this level is an important initial generator of macroevolutionary patterns across species. here, we examine how early life-history decisions and sex are related to brai ...200919878498
relating juvenile spatial distribution to breeding patterns in anadromous salmonid populations.1. spatial within-population heterogeneity in density probably affects competition intensity and may have a fundamental role in shaping population dynamics and carrying capacity. this may be particularly relevant for organisms where limitations on juvenile mobility cause maternal choice of breeding locations to influence the spatial distribution of younger life stages. 2. in this study, we mapped redd locations and the resulting densities of juveniles the following year along the entire reach (9 ...201020050942
astacene in the brown trout. 194720267555
biomarker analyses in caged and wild fish suggest exposure to pollutants in an urban area with a landfill.an unexpectedly high frequency of skeletal deformations in brown trout has previously been observed in the brook vallkärrabäcken in southern sweden. environmental pollutants from storm water and leachate from an old landfill have been suggested as responsible for the observed deformations. biomarkers in farmed rainbow trout, placed in tanks with water supplied from the brook, were used to investigate if exposure to pollutants may induce toxic responses in fish. furthermore, biomarkers were also ...201120052770
the response of intestinal mucous cells to the presence of enteric helminths: their distribution, histochemistry and fine structure.histochemical and ultrastructural investigations were conducted on the mucous cells of the intestine of brown trout, salmo trutta l., naturally infected with the cestode cyathocephalus truncatus (pallas, 1781) and the acanthocephalan echinorhynchus truttae shrank, 1788. a subpopulation of 45 s. trutta were examined of which 15 specimens harboured e. truttae, 15 of which were infected with c. truncatus and 15 fish, the control group, were uninfected. in histological sections, hyperplasia and hype ...201020298449
early-age changes in oxidative stress in brown trout, salmo trutta.fish are often used as models for studies investigating the ability of xenobiotics to induce oxidative stress, though age or developmental stage of the individuals studied has been given little attention. oxidative stress in other organisms is associated with aging as well as with periods of rapid growth, which occurs in young brown trout. we measured protein carbonyls, 20s proteosome activity and glutathione (gsh) levels in farmed salmo trutta in four different age groups from 5 months to 3 yea ...201020117229
the hepatocytes of the brown trout (salmo trutta fario): a stereological study of some cytoplasmic components with the breeding cycle.sex differences exist in fish hepatocytes, but studies for characterizing their cytology throughout the breeding cycle are still scarce; suggesting changes, but most lacking quantitative data. to address this limitation, to complement baseline data generated from the brown trout model, and to prove that sex-specific seasonal changes exist, we made an unbiased stereological evaluation of the hepatocytic cytoplasm. unprecedentedly for fish liver, the stereological design was exempt from model (bia ...201020131409
rearing unit-level factors associated with bacterial gill disease treatment in two ontario, canada government salmonid hatcheries.early-rearing salmonids in ontario ministry of natural resources (omnr) fish hatcheries have been consistently affected by bacterial gill disease (bgd) (causative agent: flavobacterium branchiophilum) for many years. separate retrospective epidemiological investigations of bgd treatments at two omnr fish hatcheries (hatcheries a and b) for the 1999 production year were conducted using on-site hatchery records. both investigations were carried out at the rearing unit-level, with early-rearing (<9 ...200919560833
the influence of altitude, hydrographic network and season on brown trout parasites in corsica using indicator species analysis.the parasites of 484 brown trout, salmo trutta, were studied between 2004 and 2007. an indicator value (indval) method was used for analysis, which combines measures of fidelity and specificity. because of its resilience to detect changes in abundance, indval is an effective ecological bio-indicator. the indval method demonstrated that altitude, hydrographic network and season could influence the occurrence of parasite species in brown trout. a randomization test identified three parasite specie ...201019566969
lysozyme activities and immunoglobulin concentrations in seminal plasma and spermatozoa of different teleost species and indications on its significance for sperm function.the occurrence of lysozyme and immunoglobulin (ig) in semen of different teleost species (brown trout-salmo trutta, perch-perca fluviatilis, burbot-lota lota) was studied. in all investigated species lysozyme activities (1.13-1.45 u ml(-1)) and ig concentrations (t-ig: 1.11-1.61 microg ml(-1), igg [measured only in brown trout]: 1.49 microg ml(-1)) were detected in seminal plasma. ig was also found in spermatozoa (t-ig: 0.234-0.357 microg/g protein, igg: 0.198 microg ml(-1)) while spermatozoal l ...201020416943
altitudinal and thermal gradients of hepatic cyp1a gene expression in natural populations of salmo trutta from high mountain lakes and their correlation with organohalogen loads.the biomarker of xenobiotic exposure cytochrome p450a1 (cyp1a) was used to analyze the biological response to chemical pollution in salmo trutta (brown trout) from nine high mountain european lakes in norway, tatras, tyrol, and central pyrenees. hepatic cyp1a mrna levels correlated both with the reciprocal of absolute annual average air temperatures of the sampled lakes and with muscle concentrations of several hydrophobic organohalogen compounds (oc), including chlorinated polychlorobiphenyls ( ...201020149942
seminal plasma of brown trout, salmo trutta fario (l.) contains a factor able to retain iron at acid ph, typical feature of lactoferrin.blood and seminal plasma of brown trout salmo trutta fario were analyzed for their iron binding potential adopting two different methods. seminal plasma showed an iron binding capacity that was retained even if samples were exposed at acid ph, similarly to mammalian lactoferrin that binds ferric iron also at acid ph. this suggests that the iron binding capacity is determined by a factor having a lactoferrin-like activity. moreover, trout seminal plasma proteins were also analyzed in their patter ...201020170734
ecotoxicological impact of highway runoff using brown trout (salmo trutta l.) as an indicator model.the ecotoxicological impact of highway runoff on brown trout (salmo trutta l.) was studied in an in situ experiment consisting of four 24 h simulated runoff episodes. fish were maintained in 5 tanks and exposed to highway runoff from a sedimentation pond close to e6 outside the city of oslo, norway. the tanks had the following contaminant loadings during the episodes: stream water (control), pond inlet, pond outlet, pond inlet + stream water and pond outlet + stream water. opposite to road salt ...201020445854
antioxidant systems of brown trout (salmo trutta f. fario) semen.the present study characterizes the antioxidant systems of brown trout, salmo trutta, semen as supplementation of semen dilution media with antioxidants can be beneficial to improve techniques for semen storage and cryopreservation. antioxidants and oxidant defensive enzymes of spermatozoa and seminal plasma were analyzed. to determine whether antioxidants and oxidant defensive enzymes have an effect on sperm functionality, in vitro experiments were performed. selected antioxidants and oxidant d ...201020171027
occurrence and variation of egg cannibalism in brown trout salmo trutta.egg cannibalism is a common behavior among fish taxa and is largely studied in species with parental care. heterocannibalism and filial cannibalism have both been reported in salmonids, a group with no extended parental care, but the topic remained somewhat under-documented, especially in brown trout (salmo trutta). in the present study, 83 spawning events were recorded finely with high-resolution video in three natural populations. redd covering dynamics by females and the timing of cannibalism ...201020195564
parasitic fauna of the lake brown trout, salmo trutta lacustris (salmonidae), a little known endemic fish from polish waters.the lake brown trout is a salmonids fish regarded as a stationary form of the migratory trout. within 2003-2004, 31 lake brown trout (salmo trutta lacustris l.) from lake wdzydze (poland), were examined for the presence of parasites following commonly used procedures. the parasites found represented digenea: diplostomum sp., posthodiplostomum cuticola (nordmann, 1832), sphaerostomum globiporum (rudolphi, 1802); cestoda: eubothrium crassum (bloch, 1779), triaenophorus nodulosus (pallas, 1781); ac ...200920209824
a hierarchical model to estimate fish abundance in alpine streams by using removal sampling data from multiple locations.the author compares 12 hierarchical models in the aim of estimating the abundance of fish in alpine streams by using removal sampling data collected at multiple locations. the most expanded model accounts for (i) variability of the abundance among locations, (ii) variability of the catchability among locations, and (iii) residual variability of the catchability among fish. eleven model reductions are considered depending which variability is included in the model. the more restrictive model cons ...201020213740
identifying footprints of selection in stocked brown trout populations: a spatio-temporal approach.studies of interactions between farmed and wild salmonid fishes have suggested reduced fitness of farmed strains in the wild, but evidence for selection at the genic level is lacking. we studied three brown trout populations in denmark which have been significantly admixed with stocked hatchery trout (19-64%), along with two hatchery strains used for stocking. the wild populations were represented by contemporary samples (2000-2006) and two of them by historical samples (1943-1956). we analysed ...201020345684
modeling future acidification and fish populations in norwegian surface waters.despite great progress made in the past 25 years, acid deposition continues to cause widespread damage to the environment in europe and eastern north america. legislation to limit emissions of sulfur and nitrogen compounds in europe is now under revision. the most recent protocol was based in part on the critical loads concept. the new protocol may also take into consideration the time delays between dose and response inherent in natural ecosystems. policy decisions to reduce adverse effects on ...201020568744
patterns of ichthyobodo necator sensu stricto infections on hatchery-reared atlantic salmon salmo salar in norway.infection patterns with ectoparasitic flagellates belonging to the genus ichthyobodo were studied in an atlantic salmon salmo salar (l.) hatchery in western norway during an 11 mo period, from eyed eggs to smoltification. since the earlier species designation ichthyobodo necator (sensu lato, s.l.) has been shown to represent a complex of several species, the epizootiology of different ichthyobodo spp. is poorly known. therefore, we employed molecular methods to ascertain the specific identity of ...201020377010
prevalence of listeria, aeromonas, and vibrio species in fish used for human consumption in turkey.a total of 78 raw retail fish samples from 30 freshwater and 48 marine fish were examined for the presence of listeria, aeromonas, and vibrio species. the overall incidence of listeria spp. was 30% in freshwater samples and 10.4% in marine fish samples. listeria monocytogenes (44.5%) was the most commonly isolated species in freshwater fish, and listeria murrayi (83.5%) was the most commonly isolated species in marine fish samples. motile aeromonads were more common in marine fish samples (93.7% ...201020132688
exposure of brown trout (salmo trutta l.) to tunnel wash water runoff--chemical characterisation and biological impact.washing and cleaning of road tunnels are a routinely performed maintenance task, which generate significant amount of polluted wash-water runoff that normally is discharged to the nearest recipient. the present study was designed to quantify chemical contaminants (trace metals, hydrocarbons, pah and detergents) in such wash water and assess the short term impact on brown trout (salmo trutta l.) based on in situ experiments. selected endpoints were accumulation of trace metals in gills, haematolo ...201020381128
cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout.the relevance of immune-endocrine interactions to the regulation of ovarian function in teleosts is virtually unexplored. as part of the innate immune response during infection, a number of cytokines such as tumor necrosis factor alpha (tnf alpha) and other immune factors, are produced and act on the reproductive system. however, tnf alpha is also an important physiological player in the ovulatory process in mammals. in the present study, we have examined for the first time the effects of tnf al ...201020385004
genetic divergence between morphological forms of brown trout salmo trutta l. in the balkan region of macedonia.the objective of this study was to characterize the genetic structure of two balkan brown trout morphotypes, salmo macedonicus and salmo pelagonicus, and to test whether molecular traits support the species' status proposed by traditional morphological identification. the mitochondrial dna 12s-rdna, cyt b and control region genes were sequenced in 15 specimens collected from three localities in the former yugoslav republic of macedonia. the results of these markers did not support the taxonomic ...201020409173
mating patterns and determinants of individual reproductive success in brown trout (salmo trutta) revealed by parentage analysis of an entire stream living population.reproductive success and its determinants are difficult to infer for wild populations of species with no parental care where behavioural observations are difficult or impossible. in this study, we characterized the breeding system and provide estimates of individual reproductive success under natural conditions for an exhaustively sampled stream-resident brown trout (salmo trutta) population. we inferred parentage using a full probability bayesian model that combines genetic (microsatellite) wit ...201020629953
use of histopathological indicators on chub (leuciscus cephalus) and brown trout (salmo trutta fario) in evaluating river environments.a multidisciplinary study was conducted on water from two rivers in the abruzzo region of italy. the study highlighted the importance of histopathological investigations in the evaluation of the environmental impact on fish. brown trout (salmo trutta fario) from the aterno river and chub (leuciscus cephalus) from the vomano river were sampled in winter and then again in spring. histopathological investigations of gills, kidneys and livers revealed inflammatory and degenerative lesions, early war ...200520437378
density dependence constrains mean growth rate while enhancing individual size variation in stream salmonids.the objective of this study was to elucidate the effects of density dependence on the individual size variation of brown trout (salmo trutta) juveniles. recruitment (the abundance of the youngest juveniles in may when they were 2 months old); the mean size attained by those individuals in september (6 months old) and the corresponding size variability around the mean size quantified with the coefficient of variation (cv) were examined in 22 year-classes at seven sites of two contrasting tributar ...201020440514
density-dependent growth rate in an age-structured population: a field study on stream-dwelling brown trout salmo trutta.a field experiment during autumn, winter and spring was performed in a small stream on the west coast of sweden, aiming to examine the direct and indirect consequences of density-dependent intercohort competition in brown trout salmo trutta. individual growth rate, recapture rate and site fidelity were used as response variables in the young-of-the-year (yoy) age class, experiencing two different treatments: presence or absence of yearlings and over-yearlings (age > or = 1+ year individuals). yo ...200920735548
latitudinal and altitudinal growth patterns of brown trout salmo trutta at different spatial scales.spatial variation in growth of stream-dwelling brown trout salmo trutta was explored in 13 populations using a long-term study (1993-2004) in the bay of biscay drainage, northern spain. the high variability in fork length (l(f)) of s. trutta in the study area was similar to the body-size range found in the entire european distribution of the species. mean l(f) at age varied: 0+ years, 57.4-100.7 mm; 1+ years, 111.6-176.0 mm; 2+ years, 155.6-248.4 mm and 3+ years, 194.3-290.9 mm. average l(f) at ...200920735558
short-term dominance: stability and consequences for subsequent growth.dominance status was determined among groups of four fish by using individuals from eight brown trout salmo trutta populations. subsequent growth of the fish was later recorded in larger groups. seven months after the first set of trials, an additional set of dominance trials was performed by using the same fish. social status affected subsequent growth; individuals having the lowest ranks grew less when compared to the higher ranking fish. furthermore, the short term dominance hierarchy was rat ...200920735559
indirect benefits for female salmon from mating with brown trout.by genetic analysis of 1625 samples from 10 south european rivers, we have found that atlantic salmon salmo salar hybridize with sympatric brown trout s. trutta in the wild and provide the female in most heterospecific crosses. hybrids exhibit reduced fertility and could be considered a wasted reproductive effort by salmon females. in 7 experiments involving salmon females, large brown trout males, and small salmon male sneakers, reproductive success of atlantic salmon females mating with brown ...201020453033
evidence for interspecific hybridization between native white-spotted charr salvelinus leucomaenis and non-native brown trout salmo trutta on hokkaido island, japan.hybrids between native white-spotted charr salvelinus leucomaenis and non-native brown trout salmo trutta were identified in streams of hokkaido, japan, using both appearance and genetic characters. the dna analyses indicated that the specimens were hybrids between female s. leucomaenis and male s. trutta. occurrence of such hybrids implies increased mating opportunities between these species in wild streams.200920735572
migratory timing, marine survival and growth of anadromous brown trout salmo trutta in the river imsa, norway.the aim of the paper was to study sea migration, growth and survival of brown trout salmo trutta of the river imsa, 1976-2005. the migratory s. trutta were individually tagged and fish leaving or entering the river were monitored daily in traps located near the river mouth. the mean annual duration of the sea sojourn was 6-9 months for first-time migrants moving to sea between january and june. it was 8-18 months for those migrating to sea between july and december. veteran migrants stayed 12 mo ...200920735583
less is more: density influences the development of behavioural life skills in trout.theory suggests that habitat structure and population density profoundly influence the phenotypic development of animals. here, we predicted that reduced rearing density and increased structural complexity promote food search ability, anti-predator response and the ability to forage on novel prey, all behavioural skills important for surviving in the wild. brown trout were reared at three densities (conventional hatchery density, a fourth of conventional hatchery density and natural density) in ...201020462903
the growth of brown trout (salmo trutta linn.); the effect of temperature on the growth of two-year-old trout. 194620988219
the selection of the 'wild': a combined molecular approach for the identification of pure indigenous fish from hybridised populations.the identification of pure indigenous fish from hybridised populations represents a key issue in fisheries management and conservation biology. in the present study an approach for selection of purebred marble trout (salmo trutta marmoratus c.) individuals out of admixed populations was set up and assessed. in a first step, baseline data sets of pure marble trout and pure brown trout specimens based on twelve microsatellite loci were used to simulate five consecutive generations of admixture. th ...200820483205
quantitative genetic parameters for wild stream-living brown trout: heritability and parental effects.adaptability depends on the presence of additive genetic variance for important traits. yet few estimates of additive genetic variance and heritability are available for wild populations, particularly so for fishes. here, we estimate heritability of length-at-age for wild-living brown trout (salmo trutta), based on long-term mark-recapture data and pedigree reconstruction based on large-scale genotyping at 15 microsatellite loci. we also tested for the presence of maternal and paternal effects u ...201020524953
exposure of ova to cortisol pre-fertilisation affects subsequent behaviour and physiology of brown trout.even before fertilisation, exposure of ova to high levels of stress corticosteroids can have significant effects on offspring in a variety of animals. in fish, high levels of cortisol in ovarian fluid can elicit morphological changes and reduce offspring survival. whether there are other more subtle effects, including behavioural effects, of exposure to cortisol pre-fertilisation in fish is unclear. here i demonstrate that a brief (3h) exposure of brown trout eggs to a physiologically relevant ( ...201020546742
habitat use and dispersal of post-smolt sea trout salmo trutta in a scottish sea loch system.post-smolt anadromous brown trout salmo trutta, sea trout, from two scottish west coast rivers, the balgy and shieldaig, flowing into adjacent sea lochs were tracked simultaneously using arrays of moored acoustic receivers to determine dispersal patterns and loss rates. fish tended to stay close to their natal rivers for the first 14 day after entering the sea, during which time about half the fish were lost to the study. although initially the overall pattern of dispersal was similar for indivi ...200920735584
an assessment of the fine sediment dynamics in an upland river system: inca-sed modifications and implications for fisheries.there is a need for better links between hydrology and ecology, specifically between landscapes and riverscapes to understand how processes and factors controlling the transport and storage of environmental pollution have affected or will affect the freshwater biota. here we show how the inca modelling framework, specifically inca-sed (the integrated catchments model for sediments) can be used to link sediment delivery from the landscape to sediment changes in-stream. inca-sed is a dynamic, proc ...201020347117
socializing makes thick-skinned individuals: on the density of epidermal alarm substance cells in cyprinid fish, the crucian carp (carassius carassius).in cyprinid fish, density of epidermal club cells (i.e. alarm substance cells) has been found to vary between lakes with different predator fauna. because predators can be labelled with chemical cues from prey, we questioned if club cell density could be controlled indirectly by predators releasing prey cues. in particular, we suspected a possible feedback mechanism between chemical alarm signals and their cellular source. we raised crucian carp singly and in groups of four. for both rearing typ ...201020582591
evidence of source-sink metapopulations in a vulnerable native galaxiid fish driven by introduced trout.introduced predators with patchy distributions can create demographic sinks within native prey populations. such invasions may give rise to source-sink metapopulations if there are still sources of native species colonists in the landscape. in new zealand, introduced brown trout (salmo trutta) and rainbow trout (oncorhychus mykiss) are linked with declines in native non-diadromous galaxiids but co-occur with these galaxiids in some locations. we investigated whether trout create sinks in galaxia ...201020597283
a multivariate comparison of the stress response in three salmonid and three cyprinid species: evidence for inter-family differences.the response of six species of freshwater fishes, from the families cyprinidae (common carp cyprinus carpio, roach rutilus rutilus and chub leuciscus cephalus) and salmonidae (rainbow trout oncorhynchus mykiss, brown trout salmo trutta and arctic charr salvelinus alpinus), to a standardized stressor was evaluated. a 6 h period of confinement resulted in changes to plasma cortisol, glucose, amino acid and lactate levels compared with unconfined controls. there were significant differences in the ...201020666900
thermal growth performance of juvenile brown trout salmo trutta: no support for thermal adaptation hypotheses.using thermal growth data from eight populations of anadromous and lake-feeding brown trout salmo trutta, hypotheses of adaptation to local optima and countergradient variation in growth were tested. the adaptation to local optima hypothesis suggests that natural selection can shift optimal performance temperatures to match the prevailing temperature in a new or changed thermal niche. in contradiction, the countergradient variation hypothesis suggests that populations from hostile environments p ...200920735529
evidence of two contrasting brown trout salmo trutta populations spatially separated in the river borne (france) and shift in management towards conservation of the native lineage.a multidisciplinary study was made of brown trout salmo trutta in the borne river, a typical fast-flowing mountain stream in the northern french alps, in the geographical range of the mediterranean lineages (ml). information on (1) the proportion of stocked fluoro-marked fish in the angling harvest, (2) the introgression of introduced dna microsatellite alleles into the native gene pool and (3) the demography of the population in situ in autumn revealed two contrasting populations separated by a ...200920735620
complete mitochondrial control region sequences indicate a distinct variety of brown trout salmo trutta in the aral sea.complete sequencing of the mtdna control region (cr) from five specimens of brown trout salmo trutta from the amu darya river identified two novel haplotypes belonging to the danubian lineage. this finding supports the long-standing hypothesis that brown trout in the aral sea represent a distinct genetic stock and also illustrates the benefits that complete sequencing of the cr can provide for elucidating phylogeographic relationships.200920735624
sibling-size variation in brown trout salmo trutta in relation to egg size and stream size.sibling-size variation (ssv), estimated as the coefficient of variation of egg size, was investigated for 13 populations of brown trout salmo trutta. ssv was negatively correlated with mean egg size both at the population and individual levels. after correction for the effect of mean egg size, ssv was also negatively correlated with stream size. these results provide new information about how salmonid ssv can vary at different ecological scales (individual, population and region). the results ar ...200920735629
a review of the likely effects of climate change on anadromous atlantic salmon salmo salar and brown trout salmo trutta, with particular reference to water temperature and flow.the present paper reviews the effects of water temperature and flow on migrations, embryonic development, hatching, emergence, growth and life-history traits in light of the ongoing climate change with emphasis on anadromous atlantic salmon salmo salar and brown trout salmo trutta. the expected climate change in the atlantic is for milder and wetter winters, with more precipitation falling as rain and less as snow, decrease in ice-covered periods and frequent periods with extreme weather. overal ...200920738500
characterization of an atlantic salmon salmo salar stream at the southern limit of its eastern atlantic distribution.ten reaches of an atlantic stream located in north-west spain were sampled intensively during one summer to characterize the conditions where atlantic salmon salmo salar have been re-introduced along the stream. fish species richness and diversity showed a downstream increase, which was mainly attributed to the higher number of cyprinid species found in the lower reaches. moreover, except for brown trout salmo trutta that appeared to be the most ubiquitous species, the densities of the other spe ...200920738507
the volitional travel speed varies with reproductive state in mature female brown trout salmo trutta.this study tested the effect of reproduction on the volitional travel speed of mature female brown trout salmo trutta l. the downstream travel speed in the pre-spawning state was 0.25 m s(-1) (95% ci : 0.19, 0.34) while it increased significantly to 0.65 m s(-1) (95% ci: 0.49, 0.87) in the post-spawning state. the results suggest state-dependent travel speed in s. trutta.200920738586
assessment of digestive enzymes activity during the fry development of the endangered caspian brown trout salmo caspius.the study of digestive enzymes activity at salmo caspius fry showed that enzymes were available at the moment of mouth opening on the first day post hatching (dph) and the activity of enzymes showed no significant difference from the hatching day 28 dph. an increased activity was seen between 32 and 43 dph and this activity was significantly higher than the activity during the first 28 days. in the primary stages after yolk sac resorption (43-58 dph), enzymes activity showed an increased profile ...200920738590
phylogeographic structure of brown trout salmo trutta in britain and ireland: glacial refugia, postglacial colonization and origins of sympatric populations.the phylogeographical structure of brown trout salmo trutta in britain and ireland was studied using polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) analysis of four mitochondrial dna segments (16s/nd1, nd5/6, coxiii/nd5 and nd5/12s). analysis of 3636 individuals from 83 sites-morphotypes revealed a total of 25 haplotypes. these haplotypes were nested in seven two-step clades. although there was a clear geographical patterning to the occurrence of derived clades, ad ...201020738710
investigation of the estrogenic risk to feral male brown trout (salmo trutta) in the shannon international river basin district of ireland.the estrogenic potential of sewage treatment effluents and their receiving waters in the shannon international river basin district (sirbd) of ireland was investigated. an integrated approach, combining biological and chemical methods, was conducted to assess 11 rivers adjacent to sewage treatment plants (stps) and their possible interference with the endocrine system of feral brown trout (salmo trutta). hepatosomatic index, gonadosomatic index, condition factor, histological (intersexuality) an ...201020810166
lactococcus lactis subsp. tructae subsp. nov. isolated from the intestinal mucus of brown trout (salmo trutta) and rainbow trout (oncorhynchus mykiss).the species lactococcus lactis currently includes three subspecies isolated from milk sources (l. lactis subsp. lactis and l. lactis subsp. cremoris) and from the leafhopper hordnia circellata (l. lactis subsp. hordniae). in this study, we isolated three strains from the intestinal mucus of brown trout (salmo trutta) and rainbow trout (oncorhynchus mykiss) closely related to this species. the 16s rrna gene of the type strain l105t showed 99.4% similarity with respect to the type strains of the s ...201020833888
histological and stereological characterization of brown trout (salmo trutta f. fario) trunk kidney.the large variability in kidney morphology among fish makes it difficult to build a "universal" model on its function and structure. therefore, a morphological study of brown trout trunk kidney was performed, considering potential seasonal and sex effects. three-year-old specimens of both sexes were collected at four stages of their reproductive cycle. kidney was processed for light and electron microscopy. the relative volumes of renal components, such as renal corpuscles and different nephron ...201020932361
genetic structure of brown trout (salmo trutta) populations from turkey based on microsatellite data.this present study investigated micro- and macro-geographic microsatellite dna variations using five polymorphic microsatellite loci from 27 brown trout populations in turkey. average number of alleles and average observed heterozygosity were 7.4 and 0.254, respectively. even populations from the same sea basin and river system (the so called micro-geographic regions) had unique alleles. genetic variation among the populations from macro-geographic regions (different sea basins and river systems ...201020963631
spatiotemporal trends of heavy metal concentrations in fish of the river morava (danube basin).the aim of this study was to assess mercury (hg), cadmium (cd) and lead (pb) contamination in muscle tissue of fishes over the longitudinal profile of the river morava (czech republic, danube basin) and to detect any temporal trends over the past 18 years. fish samples were collected in 1992, 1998, 2000, 2003 and 2009 at 6 study sites situated just downstream of important pollution sources. chub (leuciscus cephalus l.) were selected as indicator species at 5 sites, and brown trout (salmo trutta ...201020981604
the growth of brown trout (salmo trutta linn.); factors influencing the growth of trout fry. 194620988217
the growth of brown trout (salmo trutta linn.); the growth of two-year-old trout at a constant temperature of 11.5 degrees c. 194620988218
effects of different mating scenarios on embryo viability in brown trout.mating with attractive or dominant males is often predicted to offer indirect genetic benefits to females, but it is still largely unclear how important such non-random mating can be with regard to embryo viability. we sampled a natural population of adult migratory brown trout (salmo trutta), bred them in vitro in a half-sib breeding design to separate genetic from maternal environmental effects, raised 2098 embryos singly until hatching, and exposed them experimentally to different levels of p ...201021040055
identification of calcium-binding proteins in fish seminal plasma.calcium ions play an important role in the activation of fish sperm movement. the mechanism of their binding in semen is still unknown. the goal of this study was the development of a method for identifying calcium-binding proteins in fish seminal plasma. two methods of calcium-binding proteins detection were tested with the use of quin2 and stains-all dyes. the first method was useful for the identification of calcium-binding proteins of fish seminal plasma. it consisted of proteins separation ...201021042848
temperature requirements of atlantic salmon salmo salar, brown trout salmo trutta and arctic charr salvelinus alpinus: predicting the effects of climate change.atlantic salmon salmo salar, brown trout salmo trutta (including the anadromous form, sea trout) and arctic charr salvelinus alpinus (including anadromous fish) provide important commercial and sports fisheries in western europe. as water temperature increases as a result of climate change, quantitative information on the thermal requirements of these three species is essential so that potential problems can be anticipated by those responsible for the conservation and sustainable management of t ...201021078091
evaluating environmental impact of stps situated on streams in the czech republic: an integrated approach to biomonitoring the aquatic environment.sewage water treatment plants (stps) are frequently associated with the release of xenobiotics and, consequently, with biological responses of fish to these substances. the impact of three stps situated on small streams was assessed in 2009. brown trout (salmo trutta fario l.), captured upstream and downstream of these stps, were used as biomonitors. the concentrations of 39 organic pollutants (pcbs, ocps, pbdes, hbcds, and mcs), and the biological responses related to oxidative stress (lipid pe ...201021111439
high level of population genetic structuring in lake-run brown trout, salmo trutta, of the inari basin, northern finland.rivers draining into (lake) inarijärvi, northern finland, sustain a number of lake-run brown trout, salmo trutta, populations but, as with most lake-run s. trutta systems, the level of population genetic structuring among populations is unknown. to address this and to assist fish stock management in the region, the population genetic structure of s. trutta collected from 28 sampling sites in rivers flowing into inarijärvi was studied using 13 microsatellite loci. populations were clustered into ...201021133916
visualizing a set of olfactory sensory neurons responding to a bile salt.in the present study, we exposed the olfactory epithelia of crucian carp, carassius carassius, and brown trout, salmo trutta, to dextran coupled with alexa dyes together with odorants. dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. application of the bile acid taurolithocholate, a potent and specific odorant for f ...201121147971
brown trout salmo trutta redd superimposition by spawning lampetra species in a lowland stream.reproductive interaction between sympatric lampreys and salmonids was studied. the superimposition of brown trout salmo trutta redds by spring-spawning river lamprey lampetra fluviatilis and brook lamprey lampetra planeri was examined in a small lowland stream of western lithuania. a high superimposition rate of s. trutta redds by both l. fluviatilis (up to 83%) and l. planeri (up to 48%) was found, when the spawning intensity of lampetra spp. was high. the occurrence of this phenomenon is the r ...201021155788
ice-cover effects on competitive interactions between two fish species.1. variations in the strength of ecological interactions between seasons have received little attention, despite an increased focus on climate alterations on ecosystems. particularly, the winter situation is often neglected when studying competitive interactions. in northern temperate freshwaters, winter implies low temperatures and reduced food availability, but also strong reduction in ambient light because of ice and snow cover. here, we study how brown trout [salmo trutta (l.)] respond to va ...201021198589
toxicokinetics of tetrabromoethylcyclohexane (tbech) in juvenile brown trout (salmo trutta) and effects on plasma sex hormones.technical 1,2-dibromo-4-(1,2 dibromoethyl)cyclohexane or tetrabromoethylcyclohexane (tbech) used primarily as an additive flame retardant in polystyrene foams, contains two diastereoisomers, α- and β- present in equimolar amounts. at temperatures in excess of 125°c, isomerization to two other isoforms, δ- and γ- is possible. the recent detection of tbech in the environment and studies suggesting that isomers are androgenic prompted us to examine the toxicokinetics and biochemical effects of one ...201021216340
reproduction impairment following paternal genotoxin exposure in brown trout (salmo trutta) and arctic charr (salvelinus alpinus).this work describes some consequences of paternal germ cell dna damage on the reproduction success in two fish species. male brown trout (n=31) and male arctic charr (n=28) were exposed to the model genotoxicant mms at the end of spermatogenesis to generate a significant dna damage level in mature spermatozoa (28% and 25% tail dna in trout and charr sperm, respectively, evaluated through the comet assay). sperm from each mms exposed and control fish was then used to fertilize in vitro an aliquot ...201021216351
biochemical markers for the assessment of aquatic environment contamination.the need for assessment of aquatic ecosystem contamination and of its impact on water dwelling organisms was developed in response to rising aquatic environmental pollution. in this field study, liver enzymes of phase i and phase ii of xenobiotic transformation, namely cytochrome p450, ethoxyresorufin-o-deethylase, glutathione-s-transferase and tripeptide glutathione were used to assess the contamination of the aquatic environment at different rivers in the czech republic. the indicator species ...200821218108
an assessment of the spatial scale of local adaptation in brown trout (salmo trutta l.): footprints of selection at microsatellite dna loci.local adaptation is considered a paradigm in studies of salmonid fish populations. yet, little is known about the geographical scale of local adaptation. is adaptive divergence primarily evident at the scale of regions or individual populations? also, many salmonid populations are subject to spawning intrusion by farmed conspecifics that experience selection regimes fundamentally different from wild populations. this prompts the question if adaptive differences between wild populations and hatch ...201121224872
identification of the sex chromosomes of brown trout (salmo trutta) and their comparison with the corresponding chromosomes in atlantic salmon (salmo salar) and rainbow trout (oncorhynchus mykiss).males are the heterogametic sex in salmonid fishes. in brown trout (salmo trutta) the sex-determining locus, sex, has been mapped to the end of linkage group bt-28, which corresponds to linkage group as-8 and chromosome ssa15 in atlantic salmon (salmo salar). we set out to identify the sex chromosomes in brown trout. we isolated atlantic salmon bac clones containing microsatellite markers that are on bt-28 and also on as-8, and used these bacs as probes for fluorescent in situ hybridization (fis ...201121252487
polybrominated diphenyl ethers and organochlorine compound levels in brown trout (salmo trutta) from andean patagonia, argentina.polybrominated diphenyl ethers (pbdes), polychlorinated biphenyls (pcbs), ddts and endosulfan residues were analyzed in muscle, liver, gonads, gills and stomach content of brown trout (salmo trutta) from the andean patagonia. pbdes, pcbs and ddts levels were positively correlated with lipid content, while less hydrophobic endosulfans showed a poor correlation. endosulfan levels were about 99.9% of total contaminant (highest levels in liver 500×10(3)ng g(-1) lipid weight), with a-/ß-isomers ratio ...201121272913
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