| [ultrastructure and cytochemistry of chlamydia psittaci]. | | 1971 | 4939597 |
| cycloheximide-resistant glycosylation in l cells infected with chlamydia psittaci. | l cells (mouse fibroblasts), uninfected and infected with the meningopneumonitis strain of chlamydia psittaci, were labeled with [(14)c]glucosamine, and their membranous organelles were separated by isopycnic equilibrium centrifugation of whole cell homogenates on discontinuous sucrose gradients. glycosylation of host membranes continued throughout the infection. cycloheximide almost completely inhibited glycosylation in uninfected l cells, but it only partially inhibited the process in infected ... | 1974 | 4856239 |
| interaction of l cells and chlamydia psittaci: entry of the parasite and host responses to its development. | the entry and development of chlamydia psittaci in the l cell was studied by using purified, infectious parasites at high multiplicity. entry of the parasite was accomplished by an act of phagocytosis by the host which was independent of an adsorption stage but was temperature-dependent. kinetic studies of phagocytosis performed with (14)c-amino acid-labeled, purified parasites indicated that the rate of phagocytosis was directly proportional to the multiplicity of inoculation. electron microsco ... | 1972 | 4336694 |
| pulmonary lesions in sheep following experimental infection by ehrlichia phagocytophilia and chlamydia psittaci. | | 1982 | 7068951 |
| ultrastructural studies of chlamydia psittaci 6bc variant strains. i. ultrastructure of the surface layers of egg-passaged 6bc strain. | | 1973 | 4127533 |
| [epidemiologic study of chlamydia psittaci infections in mining environment of the north of france]. | | 1982 | 7072147 |
| effects of chloramphenicol and thiamphenicol on the outcome of chlamydia psittaci infection in chick embryo. | the effects of chloramphenicol (cap) and thiamphenicol (tap) on the outcome of chlamydia psittaci infection in chick embryos were compared. cap administered along with chlamydia reduced embryo mortality rates but showed no appreciable effects when its injection was delayed. on the contrary, tap caused a high rate of embryo survival in both experimental situations. statistical analysis of the results showed that differences in the survival rates following cap and tap administration are significan ... | 1982 | 7075324 |
| chlamydia psittaci infection in companion birds examined in florida. | over a two-year period, companion birds, submitted mostly by small-animal practitioners to the animal disease diagnostic laboratory, kissimmee, florida, were examined for chlamydia. chick embryo yolk sac and mouse brain inoculations were used for isolation. chlamydiae were isolated in 58 (20%) of 287 birds examined. although twice as many birds were examined in 1980 as in 1979, the percentage of positive isolations was 20% for each year. this suggests a constant level of chlamydial infection in ... | 1982 | 7092742 |
| [chlamydial infection in pericarditis in pigs]. | an etiologic study was carried out for the presence of chlamydia in the case of pericarditis in pigs on a large swine-breeding complex. investigated were some clinical, epizootiologic, and morphologic aspects of the disease. a strain of chlamydia psittaci was isolated in chick embryos from pericardial liquids of affected animals. it was identified through electron-microscopic and antigenic studies. elementary and initial bodies of chlamydia were seen in ultrathin sections of the infected yolk sa ... | 1985 | 4082488 |
| severe chlamydia psittaci sepsis in pregnancy. | two patients with severe chlamydial sepsis in pregnancy are described and compared with previously published case reports. the infections appear to have been zoonotic, the patients acquiring their infections as a result of exposure to enzootic abortion of ewes. this is an important but poorly recognised human infection which because of its severity in pregnancy deserves consideration in patients with an appropriate occupational history. | 1985 | 4080958 |
| the incidence of chlamydia psittaci antibodies in patients with respiratory tract diseases. | | 1982 | 7129645 |
| isolation and characterization of phagosomes containing chlamydia psittaci from l cells. | the obligate intracellular procaryote chlamydia psittaci enters host cells by a mechanism similar to, but distinct from, conventional phagocytosis. to better understand chlamydial uptake, l-cell phagosomes containing a single chlamydial cell were isolated and studied. two rounds of dextran rate-zonal gradient centrifugation of l cells homogenized 1 h after infection with c. psittaci yielded phagosomes relatively free of other membranous structures. in double-label experiments, the phagosomes wer ... | 1982 | 7141694 |
| role of cell-mediated immunity in chlamydial infection: implications for ocular immunity. | cellular immunity to chlamydia trachomatis is not well understood. studies with the mouse pneumonitis agent in a mouse pneumonia model suggest a strong thymic component to immunity and a role for the macrophage as an effector cell in cell-mediated immunity to c. trachomatis. experiments with chlamydia psittaci indicate that cellular cytotoxic mechanisms and biostatic or cytotoxic mechanisms involving cytokines may also play a role in host defense against chlamydiae. interferon has been shown to ... | 1985 | 4070909 |
| structural analysis of chlamydial major outer membrane proteins. | the primary structure and surface exposure of the major outer membrane protein (momp) isolated from 14c intrinsically or 125i extrinsically radiolabeled chlamydia trachomatis serotypes d/uw-3, g/uw-57, h/uw-4, i/uw-12, and l2/434 and the chlamydia psittaci meningopneumonitis strain were analyzed by two different peptide-mapping techniques. radiolabeled proteins were digested with either staphylococcus aureus v8 protease, the patterns of peptide fragments produced being displayed by sodium dodecy ... | 1982 | 7152681 |
| differences in outer membrane proteins of the lymphogranuloma venereum and trachoma biovars of chlamydia trachomatis. | the lymphogranuloma venereum (lgv) and trachoma biovars of chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. to identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (omps) by means of isoelectric focusing and nonequilibrium ph gradient electrophoresis. analysis of proteins of whole elementary bodies biosynthetically labeled with l-[35s]cysteine revealed that most chlamydial proteins were neu ... | 1985 | 4055030 |
| efficacy of a bacterin prepared from chlamydia psittaci grown in cell culture for experimental immunization of ewes. | the efficacy of a bacterin prepared from chlamydia psittaci grown in mouse l-cells was compared to a similar bacterin prepared from c. psittaci grown in chicken embryos (ce). both bacterins significantly reduced (p less than 0.03) incidence of abortion and weak lambs compared to non-vaccinated control ewes. the l-cell bacterin elicited a greater antibody response than the ce bacterin (p less than 0.03). | 1982 | 7164341 |
| chlamydia psittaci infection in a man and his birds. | | 1985 | 4044876 |
| infectivity of chlamydia psittaci of bovine and ovine origins for cultured cells. | the infectivity of 2 strains of chlamydia psittaci of mammalian origin were studied in mouse l cells. infectivity was enhanced by centrifuging the chlamydial inoculum onto the cell monolayer. infectivity increased as force of centrifugation increased. the enhanced infectivity was not caused by centrifugal sedimentation of chlamydiae, since centrifugation longer than 10 minutes and an inoculum dose larger than 0.4 ml did not further enhance infectivity. centrifuge-enhanced adsorption was temperat ... | 1982 | 7181187 |
| serological differentiation of legionnaires' disease and mycoplasma pneumoniae and chlamydia psittaci pneumonia. | | 1981 | 7185948 |
| identification of a major envelope protein in chlamydia spp. | a major cell envelope protein of chlamydia psittaci with a molecular weight of approximately 43,000 was identified and partially characterized. it was present at all stages of the c. psittaci developmental cycle. a major protein with a similar molecular weight was also observed in two chlamydia trachomatis strains. | 1981 | 7217005 |
| inapparent chlamydial infection in the urogenital tract of guinea-pigs. | microbiologically inapparent urogenital infection appeared to be induced in male guinea-pigs inoculated intra-urethrally with low doses of the guinea-pig inclusion conjunctivitis strain (gp-ic) of chlamydia psittaci. this state was indicated by the ability of inoculated animals to donate eye infection to normal animals caged with them. donors failed to develop overt urogenital infection throughout the period of transmission judged by both absence of infected cells in urethral scrapings and failu ... | 1980 | 7229613 |
| [susceptibility of the macrophages of inbred strain mice to chlamydia psittaci]. | chlamydia psittaci was injected in the peritoneal cavity of different strains of inbred mice. not all of them develop a clinical disease; some, such as c 3 h, are very susceptible and died within 6 days. c 57 bl, however, are resistant even to a very high dose. we still do not know the exact mechanism of the natural resistance. this is the reason why we are trying to analyse the role of the macrophage. this cell is particularly important in c. psittaci infection, because it harbours the obligate ... | 1983 | 6871493 |
| [chlamydia psittaci infections (psittacosis)]. | | 1981 | 7253739 |
| serological observations on chlamydia psittaci infection in finnish dairy herds. | | 1981 | 7258022 |
| bovine mastitis induced by a common intestinal chlamydia psittaci strain. a pathogenetic and serological investigation. | | 1981 | 7258034 |
| ocular and dermal delayed hypersensitivity reactions in guinea-pigs following infection with guinea-pig inclusion conjunctivitis agent (chlamydia psittaci). | guinea-pigs which had been sensitized to guinea-pig inclusion conjunctivitis agent by ocular infection, were given ocular and dermal challenges with a range of doses of agent. ocular delayed-type hypersensitivity responses were elicited by a wide range of doses. such responses were associated with the presence of chlamydial inclusions and inflammatory cells. the eid50 for ocular reinfection was only slightly higher than that for primary ocular infection. dermal delayed-type hypersensitivity reac ... | 1981 | 7261478 |
| [inapparent persistent chlamydial infection in mccoy cells]. | we have studied the phenomena of inapparent chlamydial infections in vitro using mccoy cells in culture as hosts and low doses of chlamydia psittaci (gp-ic) as infecting agents. the results indicate that gp-ic can persist undetected at least 220 hrs in mccoy cells previously inoculated with 0.2 ml of a gp-ic preparation containing 30 ifu/ml, using the centrifuge-assisted inoculation technique. these cells were maintained in complete mem medium supplemented with 2% v/v of foetal bovin serum (fbs) ... | 1981 | 7280303 |
| [comparative studies of buffalo green monkey (bgm) cells and mice for the isolation of chlamydia psittaci from avian droppings and organ samples]. | | 1981 | 7293558 |
| attachment defect in mouse fibroblasts (l cells) persistently infected with chlamydia psittaci. | almost all the cells in populations of mouse fibroblasts (l cells) persistently infected with the 6bc strain of chlamydia psittaci were immune to superinfection with high multiplicities of c. psittaci, whether or not the l cells contained visible chlamydial inclusions. as ascertained by experiments with 14c-labeled c. psittaci, immunity to superinfection resulted from the failure of added chlamydiae to attach to persistently infected host cells. however, when exogenous c. psittaci was introduced ... | 1981 | 7298188 |
| severe hepatic damage after acetaminophen use in psittacosis. | a 63-year-old man with acute psittacosis had severe hepatic damage after ingesting about 10 g of acetaminophen over a 48 hour period. transaminase levels showed striking elevation, with a serum glutamic-oxaloacetic transaminase level of over 15,000 iu/liter, and decreased rapidly, consistent with a toxic insult. no other etiologic agents were identified by history or serologic testing to explain this degree of damage. liver histologic findings at autopsy showed severe central necrosis. although ... | 1983 | 6824012 |
| localization of chlamydial group antigen in mccoy cell monolayers infected with chlamydia trachomatis or chlamydia psittaci. | chlamydial inclusions were demonstrated by indirect immunofluorescence (if) with antiserum to the chlamydial group antigen when mccoy cell monolayers infected with either chlamydia trachomatis or chlamydia psittaci were fixed in formaldehyde or paraformaldehyde, provided the monolayer was not allowed to dry. if these monolayers were then air dried and restained by if with the same antiserum but with a different fluorescence conjugate, group antigen associated with inclusion-containing mccoy cell ... | 1981 | 7309240 |
| attachment of chlamydia psittaci to formaldehyde-fixed and unfixed l cells. | the attachment of chlamydia psittaci, strain 6bc, to formaldehyde-fixed and unfixed l cells was studied. cations were found to be required for attachment to both fixed and unfixed cells. the requirement for cations was largely eliminated when the net negative surface charge on fixed cells was reduced. a high concentration of sodium chloride (0.5 m) prevented binding and removed chlamydiae which were attached to fixed and unfixed cells, whereas non-ionic detergents had no effect on attachment of ... | 1981 | 7320701 |
| infection of lambs by orally administered ovine abortion strain of chlamydia psittaci. | | 1981 | 7336547 |
| [isolation of chlamydia psittaci and moraxella bovis from infectious keratoconjunctivitis in lambs]. | out of 189 lambs in the flock, 25 animals suffered from bilateral or unilateral conjunctivitis, or keratoconjunctivitis. by serological examination (rvk), positive levels of antibodies to the group-specific antigen of chl. psittaci were found in three out of six lambs examined by laboratory methods. bacteriological examination of eye smears of six lambs showed in four cases the infection by microorganisms of moraxella bovis. smears from the conjunctivas of these lambs were after preparation inst ... | 1982 | 6814042 |
| isolation of chlamydia psittaci from imported psittacine birds in japan. | | 1981 | 7339143 |
| [isolation of chlamydia psittaci from bull ejaculate]. | during the repeated serological examination (rvk) in five breeding bulls the positive levels of antibodies to chlamydia psittaci in titre 1 : 128 were found. in the isolation experiments the pelleted ejaculates deposited in liquid nitrogen were used. the isolation of chlamydia psittaci on yolk sacs of chicken embryos was positive in two breeding bulls. the isolated strains are labelled gn-33 and ok-107. the serological examination of blood samples was in all five breeding bulls negative on bruce ... | 1980 | 6777949 |
| experimental chlamydial pneumonia in pigs. | naturally occurring disease in pigs associated with chlamydial infections has not been reported in britain, though evidence of chlamydial challenge has been demonstrated in two separate serological surveys. an isolate of chlamydia psittaci (28/68) from an ovine pneumonia produced pneumonia in pigs following intratracheal inoculation. transient pyrexia at 24 hr was followed by increased respiratory rates and inappetance which lasted for a further 48 hr in challenged pigs. histologically acute exu ... | 1984 | 6744810 |
| [human chlamydial infections (author's transl)]. | due either to chlamydia psittaci or to chlamydia trachomatis, chlamydial infections are transmitted to man directly or indirectly via birds or mammals. directly transmitted chlamydial infections may be located in the eye (trachoma, inclusion body conjunctivitis), the urogenital tract (above all urethritis but other sites are possible) or in the ent or respiratory systems (giving rise in particular to atypical pneumonias). reiter's syndrome (urethritis, conjunctivitis, polyarthritis, enterocoliti ... | 1980 | 7383904 |
| fatal pulmonary psittacosis and aspergillosis. case report of dual infection. | a 59-year-old man in previously good health had acute, progressive bronchopneumonia and died after a three-week course of illness, despite treatment with various antibiotics. serologic, microbiologic, and histologic findings indicated infection with both chlamydia psittaci and aspergillus fumigatus. disseminated, invasive aspergillosis sometimes is a complication of chronic respiratory disease, malignancy, or other deficiency of host defenses, but to our knowledge, this is the first report of ps ... | 1980 | 7396594 |
| a survey of the conjunctival flora of clinically normal cats and cats with conjunctivitis. | conjunctival swabs obtained from 39 cats with conjunctivitis and from 50 cats with clinically normal conjunctivae were cultured for bacteria, mycoplasmas, viruses and chlamydiae. non hemolytic streptococci and staphylococcus epidermidis were isolated from both groups, but b hemolytic streptococci, rhinotracheitis (feline herpes i) virus, mycoplasma felis and chlamydia psittaci were recovered only from cases of conjunctivitis. the isolation rate of microorganisms was low; only two of 50 normal an ... | 1980 | 7427854 |
| prevalence of antichlamydial antibody in london blood donors. | the prevalence of type-specific antichlamydial antibody in a population of blood donors in london was studied using a microimmunofluorescence test. twenty-six (17%) of 150 women and 38 (26%) of 150 men had antichlamydial antibody (igg at greater than or equal to 1/16 or igm greater than or equal to 1/8 or both). of these, five (3%) women had one (0.75%) man had this antibody directed against chlamydia trachomatis serotypes d-k, responsible for genital infections, and one man had antibody to chla ... | 1980 | 7448582 |
| clinical evaluation of immunity following experimental or natural infection of ewes with chlamydia psittaci (var. ovis). | the immunity conferred by a primary infection either experimental or natural was challenged at 70 days of pregnancy by intradermal inoculation of 8 x 10(7) virulent yolk sac propagated chlamydiae. the experimental primary infection was carried out, 19 months before at 70 days of pregnancy by intravenous inoculation (pi ewes). the natural primary infection was obtained 14 months before the challenge when the ewes (pcc ewes) were placed in contact with experimentally infected ewes. the response to ... | 1980 | 7458247 |
| elementary body envelopes from chlamydia psittaci can induce immediate cytotoxicity in resident mouse macrophages and l-cells. | isolated, purified chlamydia psittaci elementary body envelopes at a high multiplicity of infection (1,000:1) are capable of inducing immediate cytotoxicity in resident mouse macrophages and 929 l-cells. | 1984 | 6735471 |
| chlamydia psittaci infection in lines of mice with high or low antibody responses. | biozzi's high (h) and low (l) antibody producer mice were i.v. infected with a suspension containing 10(4)/ml viable c. psittaci organisms. h mice humoral anti-chlamydia response was much higher than that of l mice. l mice natural resistance to infection with live chlamydia was higher than that of h mice. since the two lines exhibit inverse differences in b-cell and macrophage functions, but they are quite similar with regard to t-cell-mediated immunity, it would appear that macrophages play a m ... | 1980 | 7469387 |
| structural and polypeptide differences between envelopes of infective and reproductive life cycle forms of chlamydia spp. | significant differences in cysteine-containing proteins and detergent-related solubility properties were observed between outer membrane protein complexes of reproductive (reticulate body) and infective (elementary body) forms of chlamydia psittaci (6bc). elementary bodies harvested at 48 h postinfection possessed a 40-kilodalton major outer membrane protein and three extraordinarily cysteine-rich outer membrane proteins of 62, 59, and 12 kilodaltons, all of which were not solubilized by sodium ... | 1984 | 6690419 |
| neutralization of chlamydia psittaci with monoclonal antibodies. | neutralization of chlamydia (c.) psittaci avian strain p-1041 was examined in vitro using monoclonal antibodies (mabs). of the 10 mabs used, 6 were found to exhibit neutralizing capability. these include 3 against major outer membrane protein (momp), 1 against lipopolysaccharide (lps) and 2 against other protein molecules [90 kilodalton (kda) and 90/50 kda]. most neutralizing mabs were dependent on complement for efficient neutralization, while a strain-specific mab (2b5) against the 90 kda prot ... | 1993 | 7507199 |
| isolation and characterization of a gene encoding a chlamydia pneumoniae 76-kilodalton protein containing a species-specific epitope. | chlamydia pneumoniae is a human respiratory pathogen. unlike the other two chlamydia species, no species-specific antigen has been defined for c. pneumoniae. an immunoreactive clone containing a 0.8-kb fragment was isolated from a c. pneumoniae (ar-39) genomic library by using anti-c. pneumoniae rabbit immune serum. by southern hybridization analysis of chromosomal digests of the different chlamydia spp., the 0.8-kb fragment was shown to react specifically with c. pneumoniae. subcloning of this ... | 1994 | 7509320 |
| carbohydrate inhibition of chlamydial infectivity. | the effects of exogenous carbohydrates on chlamydial infectivity were tested. it was shown that the addition of certain carbohydrates reduced the infectivity of chlamydia psittaci in cell culture. the greatest inhibitory effects were observed by monosaccharides, disaccharides and trisaccharides, respectively. the addition of atp, but not amp, enhanced the inhibitory effect. | 1983 | 6645980 |
| [pulmonitis caused by coxiella burneti and chlamydia psittaci. 2 years of research in marche]. | this paper point to infections caused by chlamydia psittaci and coxiella burneti in a region of the middle italy (marche) from april 1981 to march 1983. 32 of 686 cases (about 5%) of pneumonia were diagnosed as q fever or ornithosis-psittacosis. the role of the research of these etiologies in routine diagnostic work on acute respiratory infections is underlined. | 1984 | 6534399 |
| immunoreactivity of the 60 kda cysteine-rich proteins of chlamydia trachomatis, chlamydia psittaci and chlamydia pneumoniae expressed in escherichia coli. | the 60 kda cysteine-rich proteins (crps) of chlamydia are developmentally regulated outer envelope proteins synthesized late in the chlamydial growth cycle. these proteins, found only on the extracellular infectious elementary bodies, elicit major antibody responses in chlamydial infection. we have cloned and expressed in escherichia coli the complete 60 kda crp genes from chlamydia trachomatis, c. psittaci and c. pneumoniae. the recombinant products were expressed as either 'native' proteins or ... | 1994 | 7522846 |
| the genus-specific lipopolysaccharide epitope of chlamydia is assembled in c. psittaci and c. trachomatis by glycosyltransferases of low homology. | chlamydiae possess a genus-specific epitope that is located on the lipopolysaccharide (lps) and is composed of a 3-deoxy-d-manno-octulosonic acid (kdo) trisaccharide of the sequence alpha kdo-(2-->8)--alpha kdo-(2-->4)-alpha kdo. in chlamydia trachomatis, this trisaccharide is biosynthetically generated through the action of a multi-functional kdo-transferase encoded by the gene gsea. gsea of chlamydia psittaci 6bc was cloned and expressed in a rough mutant (re chemotype) of escherichia coli (st ... | 1993 | 7523826 |
| dissociation of immune determinants of outer membrane proteins of chlamydia psittaci strain guinea pig inclusion conjunctivitis. | chlamydia trachomatis is an important human pathogen. research to develop a chlamydia vaccine has focused on the major outer membrane protein (momp). determinants of this protein elicit serovar-specific neutralizing antibodies which are thought to play a critical role in protective immunity. momp-specific antibody responses are highly variable in the polymorphic population. genetic factors which might influence the momp-specific immune response are consequently of particular interest. the c. psi ... | 1994 | 7525489 |
| cutaneous findings in a case of psittacosis. | a patient had severe pneumonia, respiratory failure, and disseminated intravascular coagulation caused by chlamydia psittaci. cutaneous findings included a maculopapular blanching rash, acrocyanosis, superficial venous thromboses, and splinter hemorrhages under the fingernails. the history of exposure to potential vectors of psittacosis and the presence of some or all the cutaneous findings described herein may lead to the early recognition of psittacosis. | 1984 | 6476861 |
| [chlamydial infection in mass abortions in goats]. | etiologic and clinical-and-epizootiologic studies were carried out of a chlamydial infection in goat flocks where abortions took place on a mass scale. discovered were two foci of chlamydial abortion. two strains of chlamydia psittaci were isolated from aborted fetuses and from placentas on two farms. the strains were identified both antigenically and morphologically. the serologic investigation of a total of 167 goats on 6 farms with abortions revealed that in the course of two enzootics as man ... | 1984 | 6474822 |
| [the correlation between psittacosis-ornithosis and the immune status in childhood: apropos 5 cases]. | the authors studied 5 children affected with a pneumopathy by chlamydia psittaci. they discuss the effects of a transitory immunodeficiency relative to nk cells in predisposing little patients to the infective disease. | 1995 | 7567646 |
| chlamydia psittaci infections: a review with emphasis on avian chlamydiosis. | in the first part of this article the general characteristics of chlamydia psittaci namely the history, taxonomy, morphology, reproductive cycle, metabolism and genetics are reviewed. for the taxonomy in particular, a considerable amount of new information has become available in recent years, following the application of monoclonal antibodies and restriction enzymes. using these techniques isolates of chlamydia psittaci from birds have been subdivided in different serovars, a number of isolates ... | 1995 | 7571380 |
| the crp operon of chlamydia psittaci and chlamydia pneumoniae. | one of the critical developmental events during the unique intracellular life cycle of chlamydiae is their differentiation from a metabolically active, replicative form or reticulate body (rb) to an infectious extracellular form of the organism (elementary body or eb). this process is characterized by the expression of two extraordinarily cysteine-rich envelope proteins of molecular masses 9 kda and 60 kda. we describe the molecular cloning and sequence determination of the 9 kda cysteine-rich p ... | 1995 | 7582008 |
| [kinetic study of the antibodies in of 1 and c57bl mice infected with chlamydia trachomatis and chlamydia psittaci. its application to the interpretation of the serological results in human chlamydioses]. | the kinetics of antibodies to c. trachomatis and c. psittaci was studied in of 1 and inbred c 57 bl mice, inoculated intravenously (iv) or intraperitoneally. antibodies were detected by a micro-immunofluorescence (mif) test. antibody kinetics depends on many factors (genetic particularities of the mice, route of inoculation, chlamydial strain). the response is more specific when mice are inoculated with c. trachomatis than with c. psittaci; heterospecific antibodies appear later in c 57 bl mice ... | 1984 | 6377679 |
| interaction of chlamydiae with host cells and mucous surfaces. | for chlamydiae, as obligate intracellular parasites, attachment to and ingestion by host cells are essential steps in reproduction. their attachment site appears to be heat-sensitive; it has not been correlated with any morphological entity. antibody blocks chlamydial attachment to cells and, for certain chlamydia psittaci and chlamydia trachomatis strains which are highly infective for cell cultures, n-acetylglucosamine appears to contribute to cell receptor specificity. sialic acid residues ha ... | 1981 | 6911075 |
| respiratory chlamydia: the management of an outbreak. | this investigation was undertaken in response to the occurrence of nine cases of respiratory chlamydial infection in 8 months within the district of dudley. all nine cases of respiratory chlamydia were due to chlamydia psittaci, not chlamydia pneumoniae. five cases had avian exposure, but no other aetiological factors were identified. faecal specimens were obtained from only two of the implicated birds and were negative. two local aviaries were identified as sources of implicated birds. the two ... | 1995 | 7597134 |
| serum and tear antibodies to chlamydia after reinfection with guinea pig inclusion conjunctivitis agent. | repeated inoculation of th eyes of guinea pigs with the naturally occurring chlamydia psittaci agent, guinea pig inclusion conjunctivitis (gpic), showed that animals gradually become susceptible to reinfection with the passage of time after primary infection. higher levels of serum igg antibody had a significant association with resistance to challenge inoculation only with a high dose (250 eld50) but not with a low dose (25 eld50) inoculum. with each inoculum, however, some animals with high se ... | 1981 | 7031009 |
| molecular identification of an avian strain of chlamydia psittaci causing severe keratoconjunctivitis in a bird fancier. | a healthy female bird fancier developed progressive follicular keratoconjunctivitis despite topical treatment with antibiotics and steroids. although bacterial, viral, and chlamydial cultures were negative, direct fluorescent antibody staining of conjunctival scrapings revealed chlamydial lipopolysaccharide; however, this procedure failed to detect the major outer membrane protein (momp) of chlamydia trachomatis. the polymerase chain reaction (pcr) used with species-specific primers to the momp ... | 1995 | 7619997 |
| interaction of outer envelope proteins of chlamydia psittaci gpic with the hela cell surface. | the chlamydial life cycle involves the intimate interaction of components of the infectious elementary body (eb) surface with receptors on the susceptible eukaryotic cell plasma membrane. we have developed an in vitro ligand binding assay system for the identification and characterization of detergent-extracted eb envelope proteins capable of binding to glutaraldehyde-fixed hela cell surfaces. with this assay, the developmentally regulated cysteine-rich envelope protein omp2 of chlamydia psittac ... | 1995 | 7642297 |
| spontaneous change from overt to covert infection of chlamydia pecorum in cycloheximide-treated mouse mccoy cells. | some isolates of chlamydia pecorum from sheep feces failed to produce inclusions on passage in cycloheximide-treated monolayers, but chlamydiae could be recovered several weeks later. chlamydia psittaci from sheep abortions did not show this phenomenon. | 1995 | 7642316 |
| lymphokine-mediated microbistatic mechanisms restrict chlamydia psittaci growth in macrophages. | the obligate intracellular procaryote chlamydia psittaci replicated in cultures of macrophages taken from the peritoneal cavities of unstimulated or thioglycollate-elicited a/j mice. when treated with supernatant fluids (lymphokines) from c. psittaci-immune mice spleen cells that were stimulated for 24 hr in vitro by the mitogen concanavalin a, resident macrophages supplemented with heart infusion broth (8 mg/ml) and elicited macrophages markedly suppressed intracellular chlamydial development. ... | 1982 | 7054284 |
| chlamydial psittacosis during pregnancy: a case report. | chlamydia psittaci infection typically causes a mild influenza-like illness in humans. however, during pregnancy, this disease may present with severe headache, hypoxemia, thrombocytopenia, anemia, hepatic dysfunction, and disseminated intravascular coagulation. limited reports of ovine-acquired psittacosis indicate appreciable maternal-fetal morbidity and mortality. | 1995 | 7675399 |
| viral respiratory infections in asthmatic children staying in a mountain resort. | in 32 mainly atopic children (aged 9-16 years) staying in a mountain resort the effects of viral infections on the course of asthma were studied. daily surveillance was made of respiratory infection symptoms and clinical parameters for asthma, including forced expiratory volume in 1s (fev1). serology for several respiratory viruses. mycoplasma pneumoniae and chlamydia psittaci was done. in 58 symptomatic respiratory infections (sri) 39 asthmatic exacerbations occurred (67%). eighteen sris of vir ... | 1982 | 7067764 |
| cytotoxic cells induced after chlamydia psittaci infection in mice. | the ability of spleen cells from chlamydia psittaci-infected mice to lyse c. psittaci-infected and uninfected target cell monolayers was studied. the cytotoxicity assay used was a terminal label method in which the number of adherent target cells surviving the interaction with effector cells was determined by measuring the uptake of [3h]uridine by such cells. it was observed that in the first few days postinfection (3 to 5), spleens contained cells that lysed infected and uninfected targets with ... | 1982 | 7068208 |
| association between resistance to superinfection and patterns of surface protein labeling in mouse fibroblasts (l cells) persistently infected with chlamydia psittaci. | when mouse fibroblasts (l cells) were persistently infected with chlamydia psittaci strain 6bc, they became immune to superinfection because they no longer associated with exogenous c. psittaci in a way that led to ingestion and intracellular multiplication. at the same time, the persistently infected l cells also exhibited changes in surface structure as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiographic visualization of the surface-exposed plasma membran ... | 1982 | 7068218 |
| chlamydia infection in ducks: preliminary communication. | chlamydia psittaci was isolated from cloacal swab pools from ducks in processing plants, and from cloacal and conjunctival swab pools from ducks on farms, in a small proportion of the samples examined. these isolates were difficult to passage. this difficulty was partly overcome by the use of emetine as the cell growth inhibitor. the direct complement fixation (cf) test was modified for testing duck sera by using complement at the los dose of 1.5 units. by this method, positive titres were demon ... | 1982 | 7069695 |
| surface projections of chlamydia psittaci elementary bodies as revealed by freeze-deep-etching. | the morphology of the surface projections of chlamydia psittaci elementary bodies in the intracytoplasmic inclusion was the same as that of the projections on the purified elementary bodies. each projection emerged from the center of a flower structure, which was composed of nine leaves arranged radially. | 1982 | 7096263 |
| attachment of cell walls of chlamydia psittaci to mouse fibroblasts (l cells). | (14)c-labeled cell walls of the 6bc strain of chlamydia psittaci, prepared from intrinsically labeled chlamydial cells by digestion with deoxycholate and trypsin, associated with mouse fibroblasts (l cells) in a manner comparable to that of intact c. psittaci. almost half of the host cell-associated cell walls were not dissociated by trypsin, suggesting that they had been attached and then ingested. the attachment of cell walls to l cells was inhibited by a number of treatments known to block as ... | 1982 | 7129628 |
| psittacosis centenary -- 'pneumotyphus' reviewed. | since 1979, when psittacosis, human infection with chlamydia psittaci, became a notifiable disease in south africa, only 4 cases have been reported to the state health authorities. while this is not a common illness and some cases may not be reported, there is a strong possibility that this infection is being overlooked because of lack of awareness of its wide range of manifestations. a characteristic case is described and the complications and management of psittacosis are reviewed with particu ... | 1982 | 7147128 |
| spontaneous and experimental infections of xenopus laevis with chlamydia psittaci. | chlamydial infection was suspected when widespread pyogranulomatous inflammation and large basophilic intracytoplasmic inclusion bodies were evident histopathologically in african clawed frogs (xenopus laevis) dying of a spontaneous disease of high morbidity and mortality. organism morphology was determined by electron microscopy of infected hepatic sinusoidal lining cells, and it was characteristic of the unique developmental cycle of a chlamydial agent. isolation and speciation of the organism ... | 1982 | 7162133 |
| chlamydial genital infection in addis ababa, ethiopia. a seroepidemiological survey. | a seroepidemiological survey was undertaken in addis ababa to assess the prevalence of chlamydial genital infections among patients attending a sexually transmitted diseases (std) clinic and patients with no overt genital symptoms. in the std clinic patients antibodies to chlamydia trachomatis serotypes d to k (genital types) were detected in 68 of 210 (32.4%) men and in 72 of 159 (45.3%) women, a rate of exposure as high or higher than that found in europe. serological evidence of active chlamy ... | 1982 | 7171979 |
| [legionnaires' disease. first observations in catalonia (author's transl)]. | the authors report the first two cases of legionnaires' disease from catalonia. both patients were chronic bronchitic males, and the cases were sporadic. the onset of the disease was characterized by a febrile illness with muscle and joint pains, respiratory symptoms (cough and mucous sputum production), and mental changes. there were no digestive complaints. pulmonary consolidation occurred in both patients in the left upper lobe. blood chemistries disclosed the existence of an absolute lymphop ... | 1981 | 7253729 |
| the incidence of chlamydia psittaci antibodies in patients with respiratory tract diseases. | the incidence of chlamydia psittaci infections in hamburg has been studied using the complement fixation test on 655 human sera collected from patients with respiratory tract diseases. of the 653 sera investigated, 78 (11.9%) showed antibody titers between 1:8 and 1:128. of the positive sera, 21 (16.9%) presented values of greater than or equal to 1:32. this infection is encountered more often in individuals over 21 years of age (15.9% to 23.4%) than in persons under 20 (0.8% to 6.0%). general p ... | 1981 | 7298201 |
| keratoconjunctivitis of the koala, phascolarctos cinereus, caused by chlamydia psittaci. | chlamydia psittaci was cultured from 29 of 35 koalas (phascolarctos cinereus) with keratoconjunctivitis. the disease progressed from acute to chronic stages over some months, with a known duration of at least 2 years. one recovered carrier was found. up to 29% of koalas in some populations were clinically affected. a seasonal spread of infection was indicated by the high percentage of acutely affected cases found in summer. there was no evidence of susceptibility being related to age or sex. the ... | 1981 | 7338971 |
| isolation of chlamydia psittaci from cats with conjunctivitis. | chlamydia psittaci was repeatedly demonstrated in stained smears of conjunctival scrapings from a group of cats in a single household and in 5 instances the organism was isolated by yolk sac inoculation of 6-day-old pathogen free, embryonated hen eggs. thirteen of 15 cats in the cattery developed conjunctivitis at various times over a 9-month period. the outstanding features of the disease were its severity, chronicity and refractoriness to treatment. prolonged (2 week) treatment with tetracycli ... | 1981 | 7342937 |
| modifications of the envelope of chlamydia psittaci during its developmental cycle: freeze-fracture study of complementary replicas. | examination of complementary replicas obtained by freeze-fracture of chlamydia psittaci revealed, at the level of the plasma membrane, a progressive differentiation of "crate-like formations," which likely correspond to transmembranal pores. recognition of "early" and "late" stages observed in the intermediate bodies permitted detailed study of the developmental cycle of this chlamydia. | 1980 | 7364718 |
| experimental chlamydial salpingitis in the guinea pig. | acute self-limited salpingitis developed when guinea pigs' fallopian tubes were infected with the guinea pig inclusion conjunctivitis agent (chlamydia psittaci). the disease peaked at approximately 8 days post infection and had diminished markedly by day 14. the inflammatory reaction was concentrated at the luminal surface. chlamydial replication was demonstrated and inclusions were found in epithelial cells. | 1980 | 7468681 |
| efficacy trials with tissue-culture grown, inactivated vaccines against chlamydial abortion in sheep. | vaccines containing inactivated, semi-purified antigen of ovine abortifacient chlamydia psittaci were prepared from tissue-culture grown harvests of two strains. these were evaluated for efficacy against experimentally reproduced enzootic abortion of ewes (eae), the protection index (pi) of each vaccine being calculated from three variables. trial 1 tested the adjuvants marcol 52/arlacel a, iscom matrix, novasomes and alhydrogel, each administered with a total antigen dose of 4 micrograms chlamy ... | 1995 | 7483786 |
| identification of subspecies- and serotype 1-specific epitopes on the 80- to 90-kilodalton protein region of chlamydia psittaci that may be useful for diagnosis of chlamydial induced abortion. | genus-, subspecies-, and serotype 1-specific antigens of chlamydia psittaci were characterized by immunoblot analysis, using monoclonal antibodies that recognize 2 c psittaci strains: ab7 isolated from an ewe that had aborted, and ib1 isolated from feces of a healthy ewe. genus-specific epitopes were detected on lipopolysaccharide, on a 47-kd protein, and on a 27- to 30-kd doublet. subspecies-specific epitopes were located on a 30-kd protein, and a 80- to 90-kd protein region was identified, whi ... | 1994 | 7517111 |
| enzyme immunoassay with enhanced specificity for detection of antibodies to chlamydia trachomatis. | two different methods for preventing the binding of cross-reacting antibodies to the genus-reactive chlamydial lipopolysaccharide (lps) were used to improve the specificity of an enzyme immunoassay for the determination of antibodies to chlamydia trachomatis. coated elementary bodies were treated with either sodium periodate, to oxidize the antigenic sites of the lps, or triton x-100, to extract the lps. by using these new enzyme immunoassays, the standard enzyme immunoassay, and the whole inclu ... | 1994 | 7521355 |
| architecture of the cell envelope of chlamydia psittaci 6bc. | the cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent n-lauryl sarcosinate (sarkosyl). we found, however, that the insolubility of the small (enva) and the large (envb) cysteine-rich proteins of chlamydia psittaci 6bc in sarkosyl is dependent on the maintenance of a supramolecular disulfide-cross-linked complex and is unlikely to be a valid indicator of outer m ... | 1995 | 7532170 |
| potentiation of interferon-mediated inhibition of chlamydia infection by interleukin-1 in human macrophage cultures. | one mechanism by which interferons (ifns) can inhibit chlamydial infection is by the induction of the enzyme indoleamine 2,3-dioxygenase (ido), which restricts the availability of tryptophan, which is required for chlamydial growth. other immunomodulating agents, including interleukin-1 (il-1), can interact synergistically with ifns, resulting in increased ido activity in macrophages. the objectives of this study were to establish that il-1 can enhance ifn-mediated inhibition of chlamydial growt ... | 1995 | 7537250 |
| tumor necrosis factor alpha activity in genital tract secretions of guinea pigs infected with chlamydiae. | previous studies using the guinea pig model of chlamydial genital infection demonstrated that primary infection is associated with a marked acute inflammatory response early on, while chronic inflammation appears later, at a time when the level of infection is reduced. challenge infections result primarily in a chronic inflammatory response. the stimuli that initiate inflammation and lead to tissue damage have not been defined. we investigated the possibility that tumor necrosis factors (tnfs) p ... | 1995 | 7591122 |
| an oligomer of the major outer membrane protein of chlamydia psittaci is recognized by monoclonal antibodies which protect mice from abortion. | monoclonal antibodies (mabs) were generated against an ovine abortive strain of chlamydia psittaci. a plaque reduction assay was used to select 19 neutralizing antibodies which appeared to be heterogeneous in isotype, specificity, and recognized proteins. different neutralizing mabs were tested for their protective abilities against abortion in a pregnant-mouse model. all of the protective mabs selected had the same isotype, were serotype 1 specific, and recognized a protein of about 110 kda by ... | 1995 | 7591155 |
| prevalence of chlamydia psittaci antibodies in healthy pet cats in britain. | | 1995 | 7610543 |
| usefulness of omp1 restriction mapping for avian chlamydia psittaci isolate differentiation. | sixty-five avian chlamydia psittaci isolates collected worldwide, including 27 previously characterized reference strains, were analysed by restriction mapping of the major outer membrane protein gene (omp1) obtained after dna amplification by pcr. they were compared to 2 ruminant isolates, a feline pneumonitis and a guinea pig inclusion conjunctivitis (gpic) isolate. according to their omp1 restriction patterns, avian strains were heterogeneous in that they exhibited 6 and 4 distinct patterns u ... | 1995 | 7652209 |
| characterization of a neutralizing monoclonal antibody directed at variable domain i of the major outer membrane protein of chlamydia trachomatis c-complex serovars. | a monoclonal antibody (mab), c10, that neutralized in vitro the infectivity of serovars c, i, j, and l3 (members of the c and c-related complexes) of chlamydia trachomatis was identified. of the 15 major serovars and the mouse pneumonitis strain of c. trachomatis, chlamydia psittaci, and chlamydia pneumoniae, which were used as nontreated and heat-treated (56 degrees c, 30 min) antigens in a dot blot assay, only serovars c, i, j, and l3 were recognized with both the native and treated antigens. ... | 1993 | 7681045 |
| some aspects of the immune response of koalas (phascolarctos cinereus) and in vitro neutralization of chlamydia psittaci (koala strains). | western-blot analysis was used to study the reaction of koala antisera, two specific polyclonal antibodies and one monoclonal antibody, with chlamydial antigens in koalas infected with chlamydia psittaci. the koala sera recognized four c. psittaci surface antigens, corresponding to the major outer membrane protein (39.5 kda), 31 kda protein, 18 kda protein and lipopolysaccharide. the s25-23 lps specific monoclonal antibody inhibited chlamydial infection (55-67%) with both koala strains (type i a ... | 1993 | 7682465 |
| chlamydial infection of the respiratory tract. | chlamydial infection of the human respiratory tract was first described over 100 years ago, when outbreaks of psittacosis were linked to imported pet birds. the causative organism was identified subsequently as chlamydia psittaci. however, c. pneumoniae, has also been recognised recently as an important cause of human respiratory tract infection. approximately 300 cases of human chlamydial respiratory tract infection are reported to cdsc each year, mainly in adults aged 15 to 44 years. a prospec ... | 1993 | 7693170 |
| [detection of chlamydia psittaci in vaginal discharge of cows: a necessary enlargement of bacteriologic diagnosis for the etiologic clarification of fertility disorders in the female cow]. | vaginal discharge from 119 dairy cows from 59 herds was examined bacteriologically, including application of the ideia chlamydia test, to detect genus-specific chlamydial lps-antigen. a putrid quality of specimens was closely correlated with isolation of actinomyces pyogenes (p < 0.001). the ideia was positive for 39 of 65 (60.0%) non-putrid specimens and for 16 of 54 (29.6%) putrid specimens; a non-putrid quality of specimens was closely correlated with the detection of chlamydial antigen (p < ... | 1994 | 7701862 |
| development and evaluation of an indirect elisa to detect antibodies to abortion strains of chlamydia psittaci in sheep sera. | a novel indirect enzyme-linked immunosorbent assay (elisa) for antibodies against abortion strains of chlamydia psittaci (c. psittaci) has been developed. the antigen used was chlamydial elementary bodies treated sequentially with n-lauroyl sarcosine and n-octyl-beta-d-glucopyranoside and finally solubilized with n-lauroyl sarcosine and dithiothreitol. treating the antigen with sodium periodate after coating of the plates increased the specificity for antibodies to abortion strains. the test was ... | 1995 | 7716879 |
| antigenic diversity of ruminant chlamydia psittaci strains demonstrated by the indirect microimmunofluorescence test with monoclonal antibodies. | monoclonal antibodies (mabs) were produced to find strain markers essential to the epidemiological study of chlamydial abortion of ruminants. their specificity was tested on 53 c. psittaci strains including 35 ruminant strains isolated mainly from abortion, belonging to serotype 1 and which are invasive in our mouse model (rodolakis et al., 1989), and 14 ruminant strains mostly intestinal, belonging to serotype 2 and non-invasive for mouse. one strain specific mab was obtained reacting only with ... | 1995 | 7740760 |
| the structures of oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant escherichia coli strain expressing the gene gsea [3-deoxy-d-manno-octulopyranosonic acid (kdo) transferase] of chlamydia psittaci 6bc. | the lipopolysaccharide from the recombinant strain escherichia coli f515-140 containing the cloned gene gsea [3-deoxy-d-manno-octulopyranosonic acid (kdo) transferase] from chlamydia psittaci 6bc was isolated and sequentially de-o-acylated and de-n-acylated. the products were separated by high-performance anion-exchange chromatography into three fractions, two of which contained a single compound. their structures were elucidated by high-field nmr spectroscopy as alpha-kdo-(2-->4)-alpha-kdo-(2-- ... | 1995 | 7744029 |
| [hemorrhagic macular choroidopathy in young adults. apropos of a case]. | we report a case of haemorrhagic macular choroidopathy in young adult occurring in a 24-year-old woman. the aetiology of the affection remains unknown in europe, but many authors suggest arterial dissemination of an infectious agent may be involved, as in the american form. in the case described here, it could be chlamydia psittaci. | 1995 | 7759762 |
| electrophoretic analysis of the major outer membrane protein of chlamydia psittaci reveals multimers which are recognized by protective monoclonal antibodies. | purified major outer membrane protein, detergent solubilized and reduced with dithiothreitol but not heated, gave an apparent molecular weight in sodium dodecyl sulfate (sds)-polyacrylamide gels almost three times that observed for the heat-denatured sds-treated peptide. this is similar to the behavior of porin trimers from gram-negative bacteria. two protective monoclonal antibodies showed strong binding to the proposed trimer but not to denatured, monomeric major outer membrane protein. | 1995 | 7768628 |
| recombinant ovine interferon gamma inhibits the multiplication of chlamydia psittaci in ovine cells. | the local production of interferon-gamma (ifn-gamma) in sheep in response to chlamydia psittaci was measured by cannulation of the efferent lymph duct draining the site of challenge inoculation. peak production of ifn-gamma (256 u/ml) was detected 24 h after challenge. based on these physiological data, functional studies were carried out in vitro to determine the effect of recombinant ovine (rov) ifn-gamma on the multiplication of c. psittaci in ovine fibroblasts. ifn-gamma inhibited the multip ... | 1995 | 7769147 |