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duck hepatitis virus and chlamydia psittaci outbreak. 19853872526
in vitro expression of factor-mediated cytotoxic activity generated during the immune response to chlamydia in the mouse.supernatant fluid (sf) prepared by mitogen incubation of spleen cells from a/j mice previously immunized against lethal challenge by the 6bc strain of chlamydia psittaci was cytotoxic for mouse fibroblasts (l cells) infected with 6bc, as detected by the [3h]thymidine release assay and the trypan blue exclusion test. in contrast, sf prepared from spleen cells taken from unimmunized animals (controls) was not cytotoxic when added to infected l cells. no cytotoxicity was observed when sf was added ...19853872911
"contribution to the question of pneumotyphus": a discussion of the original article by j. ritter in 1880.in 1880, dr. j. ritter wrote a classic infectious disease article (originally in german) on psittacosis entitled, "contribution to the question of pneumotyphus." in this article, ritter meticulously describes a mini-epidemic--in which three individuals died--of seven cases of psittacosis caused by parrots and finches caged in the study of his brother's house in uster, switzerland. ritter accurately identified the study as the site of the source of infection, considered the birds as vectors, and ...19853885364
comparative titers of egg assay against immunofluorescent assay of chlamydia psittaci.a comparison of titers was made between an egg assay and a direct fluorescent antibody assay of three chlamydial strains propagated in vero cells with and without cortisone plus cytochalasin b. the titer of nj-1 strain was similar in the egg titration and the fluorescent antibody assay in the untreated sample and a little lower for the sample treated with cytochalasin b and cortisone. the sct and cdc strains had approximately the same titers in the egg titration and the fluorescent antibody assa ...19853886106
an epidemic of mild pneumonia due to an unusual strain of chlamydia psittaci.an epidemic of mild pneumonia was discovered during a chest radiographic survey of adolescents and young adults in two communities 110 kilometers apart in northern finland. antibodies to chlamydial antigens were found in 32 of 34 persons with pneumonitis. microimmunofluorescence antibody tests suggest that the etiologic agent is closely related or identical to tw-183, an unusual strain of chlamydia psittaci isolated from the eye of a child in taiwan. the point prevalence of pneumonitis with anti ...19853886806
direct immunoperoxidase method for demonstrating chlamydia psittaci in tissue sections. 19853891793
[use of the immunofluorescent microagglutination reaction for the intrageneric differentiation of the immune response in chlamydiosis].the possibility of using, on principle, the immunofluorescent microagglutination test with chlamydia fluorescent corpuscular antigen for the strain-specific differentiation of immune response induced by the causative agents of ornithosis (strain b), enzootic abortus ovis (strain eae) and lymphogranuloma venereum (strain lv) has been experimentally demonstrated. the calculation of indices, characterizing the specificity of differences between the systems under comparison, by the method of s. fras ...19853892987
new view of the surface projections of chlamydia trachomatis.two kinds of surface specializations of chlamydiae have been described: hemispheric projections and spikelike rods. we undertook the present studies to demonstrate chlamydial ultrastructure in greater detail in conventional thin-sectioned specimens. chlamydia trachomatis (lgv strain l2/434/bu), cultured for 40 h in l929 mouse fibroblasts, was fixed in glutaraldehyde-acrolein, p-formaldehyde-glutaraldehyde, or glutaraldehyde-osmium tetroxide mixtures, postfixed in osmium tetroxide, stained in ura ...19853900041
antigenic diversity of chlamydia psittaci of mammalian origin determined by microimmunofluorescence.a group of twenty-five isolates of chlamydia psittaci representing at least seven different biotypes of bovine, ovine, caprine, equine, feline, porcine, and guinea pig origin were immunotyped by an indirect microimmunofluorescence test. different groups of chlamydia-free balb/c mice received two weekly intravenous inoculations with chicken embryo-propagated, partially purified elementary bodies of each strain. antisera for immunotyping were collected 4 days after the first inoculation and 3 to 4 ...19853905618
chlamydial infections.chlamydiae are small bacteria that have a unique life cycle. there are two species, chlamydia psittaci and c. trachomatis, which cause a wide spectrum of clinical disease, including neonatal conjunctivitis and pneumonia, sexually transmitted disease, psittacosis, and trachoma. the importance of chlamydial disease in public health is being increasingly recognized, and the incidence in developed countries seems to be increasing. an understanding of chlamydial disease, its prevention and treatment, ...19853909858
[chlamydia infections in internal medicine]. 19853910859
chlamydia immunoreactivity in birds with psittacosis: localization of chlamydiae by the peroxidase-antiperoxidase method.diagnosis of psittacosis (infection with chlamydia psittaci) has traditionally been dependent upon culture or demonstration of chlamydiae in tissues by cytochemical stains such as the gimenez or macchiavello's stain. in this study, a peroxidase-antiperoxidase (pap) technique that employs a commercially available monoclonal antibody directed against chlamydia is used to demonstrate chlamydiae in the necropsy tissues of nine birds. chlamydiae were identified in air sacs (83%), liver (78%), spleen ...19853914270
abortion due to infection with chlamydia psittaci in a sheep farmer's wife.a farmer's wife who had helped with lambing aborted spontaneously in march after a short febrile illness in the 28th week of her pregnancy. she developed disseminated intravascular coagulation post partum with acute renal failure and pulmonary oedema. recovery was complete after two weeks of hospital care. a strain of chlamydia psittaci, probably of ovine origin, was isolated from the placenta and fetus. the patient's serum showed rising titres of antibody against chlamydia group antigen; the pl ...19853918685
properties of monoclonal antibodies to the genus-specific antigen of chlamydia and their use for antigen detection by reverse passive haemagglutination.the chlamydial genus-specific antigen was extracted with phenol/chloroform/petroleum ether (pcp) from preparations of chlamydia trachomatis and c. psittaci, and quantities measured using an assay for lipopolysaccharide (lps). the lps from c. trachomatis contained 2.2% (w/w) of ketodeoxyoctanoic acid. five igg monoclonal antibodies reacted in an elisa with lps from both species, the antigen being periodate-sensitive and heat-resistant, confirming that all antibodies were against the genus-specifi ...19853921656
activation of tissue macrophages from aids patients: in vitro response of aids alveolar macrophages to lymphokines and interferon-gamma.to test the hypothesis that tissue macrophages from aids patients have no intrinsic defects in either antimicrobial activity or in the capacity to respond to t cell-derived activating stimuli, alveolar macrophages from 11 patients were treated with crude lymphokines produced by healthy donors. after 72 hr of pretreatment with 10% mitogen- or antigen-induced crude lymphokines (which contained 300 u/ml of interferon-gamma [ifn-gamma]), aids alveolar macrophages generated twofold to threefold more ...19853928746
identification and properties of chlamydial polypeptides that bind eucaryotic cell surface components.an electroblotting technique was used to identify proteins of chlamydia that bound surface-radioiodinated and triton x-100-solubilized hela cell extracts. two proteins, with apparent molecular masses of 18 and 32 kilodaltons (kda), that bound hela cell surface components were identified on chlamydia trachomatis l2 elementary bodies (ebs). radioiodinated heparin, which disrupts chlamydial association with cultured cells, was also bound by these proteins. these two proteins were found on ebs but w ...19863941041
molecular characterization of chlamydia trachomatis and chlamydia psittaci plasmids.plasmids from chlamydia trachomatis lgv-434 (serotype l2) and chlamydia psittaci meningopneumonitis strain cal-10 were cloned into the bamhi and ecori sites of pbr322, respectively. the recombinant plasmids pctl2 and pcpmn, each containing an entire respective chlamydial plasmid, were transformed into escherichia coli. the sizes of the plasmids of c. trachomatis and c. psittaci were 7.3 and 6.2 kilobases, respectively. the two plasmids were found to be distinct by restriction endonuclease analys ...19863943908
synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of chlamydia psittaci and chlamydia trachomatis.the disulfide bond cross-linked major outer membrane protein (momp) of the extracellular elementary bodies (ebs) of chlamydia psittaci was reduced to its monomeric form within 1 h of entry of ebs into host cells by a process which was inhibited by chloramphenicol, while monomeric forms of three cross-linked cysteine-rich proteins could not be detected in sarkosyl outer membrane complexes at any time in either extracellular or intracellular forms of c. psittaci. synthesis and incorporation of the ...19863944054
chlamydia psittaci infections of pet and other birds in the united states in 1984. 19863944489
cold urticaria and virus infections: a clinical and serological study in 39 patients.thirty-nine patients with cold urticaria seen over a 12-year-period were re-examined. all but 12 still had positive skin tests for cold and only five of these had shown a spontaneous cure. fourteen patients were prone to collapse on cold exposure. the incidence of atopy in this group was comparable to that in control groups. cold urticaria is an extremely chronic disease. the mean disease duration was 9.3 years. serum antibodies to epstein-barr virus, measles virus, cytomegalovirus (cmv), varice ...19863954951
respiratory disease (rhinotracheitis) in turkeys in brittany, france, 1981-1982. ii. laboratory findings.after discovering that numerous turkey flocks experiencing rhinotracheitis in brittany, france, had antibodies against chlamydia, laboratory studies were conducted to determine whether chlamydia and/or viruses would explain the respiratory disease observed. although both lentogenic paramyxoviruses of type 1 (newcastle disease virus) and chlamydia psittaci were isolated, it was concluded, based on epidemiologic and other laboratory findings, that c. psittaci was the primary cause of the disease.19853985882
[role of eye manifestations in chlamydioses].following an up-to-date bacteriological resume, are recalled the most important clinical manifestations presently attributed to c. trachomatis: general manifestations (pulmonary, articular, venereal, congenital, viscereal and others still under discussion); ocular manifestations (trachoma, inclusion conjunctivitis of newborn infants or adults, other ocular affections still under discussion). the main theories are exposed, which try to explain some epidemiological links between all these manifest ...19853990540
enigmatic psittacine chlamydiosis: results of serotesting and isolation attempts, 1978 through 1983, and considerations for the future.from 1978 through 1983, chlamydiosis was diagnosed by isolation of chlamydia psittaci from various types of psittacine birds. the organism was isolated from 126 (30.4%) of 414 tissue specimens, with the percentages ranging from 12.5% (budgerigars) to 42.8% (cockatiels), excluding 2 parakeets with 1 isolation (50%). from 1,035 cloacal swab/feces specimens, 51 (4.9%) isolations were made, ranging from 1.4% from african grays (1 of 70) to 27.8% from lovebirds (5 of 18). positive direct microscopic ...19853997621
synthesis of protein in host-free reticulate bodies of chlamydia psittaci and chlamydia trachomatis.synthesis of protein by the obligate intracellular parasitic bacteria chlamydia psittaci (6bc) and chlamydia trachomatis (serovar l2) isolated from host cells (host-free chlamydiae) was demonstrated for the first time. incorporation of [35s]methionine and [35s]cysteine into trichloroacetic acid-precipitable material by reticulate bodies of chlamydiae persisted for 2 h and was dependent upon a exogenous source of atp, an atp-regenerating system, and potassium or sodium ions. magnesium ions and am ...19853997784
rickettsiae in the sumava region.antibodies were detected to c oxiella burnetii, rickettsia slovaca and chlamydia psittaci in the blood of small terrestrial mammals, larger wild and domestic animals and humans in the southern part of the protected landscape area of the sumava region between 1977 and 1982. the data obtained have contributed to the safe development of livestock raising in this region.19854007702
acute placentitis and spontaneous abortion caused by chlamydia psittaci of sheep origin: a histological and ultrastructural study.a sheep farmer's wife who had been assisting with lambing developed an influenza like illness in the 28th week of pregnancy. after five days of malaise she spontaneously delivered a stillborn infant; she became acutely ill during the immediate postpartum period with septicaemic shock, acute renal failure, and disseminated intravascular coagulation. the diagnosis was made by isolation and identification of chlamydia psittaci from the placenta, fetal heart blood, and fetal lung, together with mate ...19854008668
[generalities on chlamydia. clinical, diagnostic and therapeutic applications]. 19854008874
'going-light' and psittacosis. 19854013038
attempted venereal transmission of chlamydia psittaci in sheep.attempts were made to transmit chlamydia psittaci, the causal agent of enzootic abortion of ewes, in three different ways. ten ewes were inseminated artificially with freshly collected semen containing 10(5) celd50 chlamydia. serological evidence of infection was found two weeks before parturition in nine ewes and the organism was recovered from three of them. by six weeks post partum antibody titres had fallen and were negative in six ewes. twenty-four hours after service two groups of 10 ewes ...19854013041
etiology of community-acquired pneumonia in patients requiring hospitalization.the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ...19854018066
assessing the number of genital chlamydial infections in the united states.the prevalence and incidence of genital infections with chlamydia trachomatis have not yet been determined for broadly based populations within the united states. largely on the basis of results of prevalence studies from sexually transmitted disease clinics, it has been estimated that c. trachomatis infections are the most common of all sexually transmitted infections and may number 3-5 million cases annually. c. trachomatis isolation rates range from 50% to 80% in certain diseases known to be ...19854020783
[detection of chlamydia in patients with trachoma]. 19854028211
electron microscopic observations concerning the in vivo uptake and release of the agent of guinea-pig inclusion conjunctivitis (chlamydia psittaci) in guinea-pig exocervix.this report details electron-microscopical observations concerning c. psittaci infection in vivo. the model employed was that of the guinea-pig infected at the exocervical region with the agent of guinea-pig inclusion conjunctivitis (gpic). our observations indicate that chlamydial particles gain access to their target cells by the mechanism of endocytosis. single gpic elementary bodies were seen to be positioned within individual endosomes. the observations reported here provide evidence that c ...19854031129
[use of a concentrated and purified vaccine against chlamydial abortion in sheep].attempts were made to immunize sheep against chlamydial abortion with a purified, concentrated, and absorbed vaccine. the latter proved innocuous and unreactive, and was applied at rates of 0.5 to 1.00 cm3. it was found that at a single vaccination of the sheep with 0.5 cm3 as many as 91.42 per cent responded with the production of antichlamydial complement-fixing antibodies on the 45th day of 1:8 to 1:512 titers; 96.43 per cent of the animals responded on the 111th day, the titers ranging from ...19854035984
establishment of hybridomas secreting monoclonal antibodies to chlamydia psittaci.eleven stable anti-chlamydia hybrid clones by fusions between x63-ag8653 myelomas and immune splenocytes from chlamydia psittaci immunized f1 (c57bl6 x balb/c) mice have been established which react with the 12 reference chlamydia strains (seven c. trachomatis and five c. psittaci. ten of these monoclonal antibodies are directed against the genera-specific epitope (40,000 mw component) for which prolonged immunization seems to be responsible.19854044019
chlamydia psittaci infection in a man and his birds. 19854044876
antibodies against chlamydia measured by an elisa method.an enzyme-linked immunosorbent assay (elisa) for the detection of serum igg and igm antibodies against chlamydia group antigen is presented. 187 selected sera were tested both in the elisa and in the complement-fixation test (cft). of 71 sera with negative results in the cft, 19 (26.8%) were positive in the elisa. of 116 cft positive sera, ten (8,6%) were elisa negative. in 35 of 72 sera (48.6%) with cft titre less than 40 chlamydial igm was demonstrated, while 11 of 115 sera (9.5%) with cft tit ...19854050443
[chlamydia infection during pregnancy]. 19854054008
persistent infection of l cells with an ovine abortion strain of chlamydia psittaci.l cells inoculated at multiplicities of infection greater than or equal to 1 inclusion-forming unit of the abortigenic chlamydial strain b577 were destroyed within 10 to 15 days. upon continued incubation in fresh medium, a few surviving cells repopulated the flasks, and the reemerging cultures remained persistently infected. the persistent state was characterized by cycles of repopulation with a low ratio of infected cells and cycles of extensive cytopathic changes in which greater than 90% of ...19854055027
differences in outer membrane proteins of the lymphogranuloma venereum and trachoma biovars of chlamydia trachomatis.the lymphogranuloma venereum (lgv) and trachoma biovars of chlamydia trachomatis exhibit differences in biological properties both in vivo and in vitro. to identify analogous biochemical differences, we studied the molecular charges of chlamydial outer membrane proteins (omps) by means of isoelectric focusing and nonequilibrium ph gradient electrophoresis. analysis of proteins of whole elementary bodies biosynthetically labeled with l-[35s]cysteine revealed that most chlamydial proteins were neu ...19854055030
pathogenesis and immunology of chlamydial infections of the genital tract.in recent years there has been a steady increase in our understanding of the pathogenesis and immunobiology of lower genital tract infections caused by chlamydia trachomatis. much of this increase in knowledge has stemmed from two areas of research: clinical, pathologic, and immunologic observations of patients and the development and study of animal models. research on patients has included the use of techniques such as histologic examination of biopsy specimens and measurement of levels of ant ...19854070907
role of cell-mediated immunity in chlamydial infection: implications for ocular immunity.cellular immunity to chlamydia trachomatis is not well understood. studies with the mouse pneumonitis agent in a mouse pneumonia model suggest a strong thymic component to immunity and a role for the macrophage as an effector cell in cell-mediated immunity to c. trachomatis. experiments with chlamydia psittaci indicate that cellular cytotoxic mechanisms and biostatic or cytotoxic mechanisms involving cytokines may also play a role in host defense against chlamydiae. interferon has been shown to ...19854070909
direct complement fixation and isolation attempts for detecting chlamydia psittaci infection of psittacine birds.serum and fecal specimens from 147 birds were tested for chlamydia psittaci. isolation rates ranged from 3.4% of 88 birds that had titers less than 8, to 50% of six birds that had titers greater than or equal to 128. testing of 44 paired sera revealed a significant titer increase in 5 pairs, a significant titer decrease in 8 pairs, and a stable titer in 31 pairs. the examination of both feces and serum is recommended for determining chlamydia psittaci infections in individual live psittacine bir ...19854074254
[pulmonary infections caused by chlamydia psittaci. clinical and epidemiological study of 31 sporadic cases attended at a general hospital]. 19854079494
severe chlamydia psittaci sepsis in pregnancy.two patients with severe chlamydial sepsis in pregnancy are described and compared with previously published case reports. the infections appear to have been zoonotic, the patients acquiring their infections as a result of exposure to enzootic abortion of ewes. this is an important but poorly recognised human infection which because of its severity in pregnancy deserves consideration in patients with an appropriate occupational history.19854080958
seroepidemiological surveillance of chlamydia psittaci in cats and dogs in japan. 19854082403
[chlamydial infection in pericarditis in pigs].an etiologic study was carried out for the presence of chlamydia in the case of pericarditis in pigs on a large swine-breeding complex. investigated were some clinical, epizootiologic, and morphologic aspects of the disease. a strain of chlamydia psittaci was isolated in chick embryos from pericardial liquids of affected animals. it was identified through electron-microscopic and antigenic studies. elementary and initial bodies of chlamydia were seen in ultrathin sections of the infected yolk sa ...19854082488
[course of chlamydia-induced inclusion conjunctivitis in the guinea pig in experimental animal husbandry]. 19854082800
[chlamydial infection with main symptoms of upper cervical lymphadenitis (ucla). isolation of chlamydiae]. 19854087073
an enzyme-linked immunosorbent assay (elisa) for the detection of chlamydial antibodies in caprine sera.chlamydia psittaci is one of the major cause of abortion in goats. the complement-fixation (cf) test is widely used for detection of chlamydial antibodies in caprine sera. a simple, rapid enzyme-linked immunosorbent assay (elisa) has been developed for the measurement of immunoglobulins g (igg). the chlamydia psittaci strain ab3 grown in yolk sac was used as antigen. elisa proved satisfactory for the detection of serum igg; a total of 204 sera were tested and the results correlated well with tho ...19854091495
morphologic and cytochemic studies on the development of chlamydia psittaci in a tissue culture of a cold-blooded animal. 19694097119
respiration of l cells infected with chlamydia psittaci. 19704099299
effect of metabolic inhibitors on the production of chlamydia psittaci by infected l cells. 19704099301
morphologic and cytochemic studies on the development of chlamydia psittaci in tissue culture of a cold-blooded animal at 20 degrees c. 19714101542
lysosomes in l cells infected with chlamydia psittaci 6bc strain. 19714106184
[phase contrast microcinematographic analysis of the intracellular development of chlamydia psittaci]. 19714109492
inapparent respiratory infection of inbred swiss mice with sulfadiazine-resistant, iodine-negative chlamydiae.three chlamydial strains were isolated from the lungs of three different strains of mice that were inbred for over 30 years. these chlamydial strains were resistant to sulfadiazine and did not induce iodine-positive, glycogen-like material in the inclusions of infected l cells which characterizes them as chlamydia psittaci.19714117289
electron microscope observations on the effects of polymixin b sulfate on cell walls of chlamydia psittaci.the effects of polymixin b sulfate on cell walls of mature elementary body (eb) and of immature developmental reticulate body (rb) of chlamydia psittaci were investigated. when purified eb were treated with polymixin (10(4) units per ml or more) at 37 c for 60 min, about 70% of eb was found to be covered with a number of projections. further incubation did not increase the percentage affected. the infectivity after treatment as assayed by the inclusion counting technique was reduced by 70% of th ...19734120068
ultrastructural studies of chlamydia psittaci 6bc variant strains. i. ultrastructure of the surface layers of egg-passaged 6bc strain. 19734127533
fine structures of cell envelopes of chlamydia organisms as revealed by freeze-etching and negative staining techniques.the cell walls of chlamydia psittaci (meningopneumonitis strain) were examined by the freeze-etching and negative staining techniques. it was observed that the cleaved convex surface of the developmental, reticulate body was covered with numerous non-etchable particles 9 to 10 nm in diameter, these particles being rarely seen on the concave surface. similarly, the convex surface of the mature, elementary body (eb) was covered with many particles but the concavity lacked these particles. after et ...19734127629
location of polysaccharide on chlamydia psittaci by silver-methenamine staining and electron microscopy.previous serological studies have indicated that the group antigen of chlamydial organisms is composed of an acidic polysaccharide and a lipid component. the present study was undertaken in an effort to locate this polysaccharide complex by use of electron microscopy and a silver-methenamine marker. the meningopneumonitis strain of chlamydia psittaci was propagated in hela-m cell culture. organisms were purified by differential centrifugation, treatment with genetron, and by gel filtration. afte ...19724130428
acquired valvular heart-disease in patients who keep pet birds. 19744143008
[ornithosis and influenza with multiple titer rises in complement-fixation (author's transl)]. 19744157072
ocular involvement with chlamydia psittaci (strain m56) in rabbits inoculated intravenously.fourteen albino rabbits were inoculated intravenously with 10(3.5)-10(4.0) mouse icld(50) of chlamydia psittaci (strain m56) of mammalian origin. ocular lesions accompanied the chlamydial infection in the rabbits. bilateral anterior uveitis, a common occurrence, began on the second or third day and subsided by the tenth day whereas keratoconjunctivitis was observed infrequently. after 15 days the most prominent microscopic lesion was iritis. accumulations of inflammatory cells, mainly plasma cel ...19744277591
properties of a chlamydia psittaci isolated from muskrats and snowshoe hares in canada. 19714328689
interaction of l cells and chlamydia psittaci: entry of the parasite and host responses to its development.the entry and development of chlamydia psittaci in the l cell was studied by using purified, infectious parasites at high multiplicity. entry of the parasite was accomplished by an act of phagocytosis by the host which was independent of an adsorption stage but was temperature-dependent. kinetic studies of phagocytosis performed with (14)c-amino acid-labeled, purified parasites indicated that the rate of phagocytosis was directly proportional to the multiplicity of inoculation. electron microsco ...19724336694
[influence of quartz, carbon, titanium oxide and asbestos dusts on the experimental disease in mice induced with chlamydia psittaci]. 19724339891
immunofluorescence of peritoneal phagocytes after infection in vivo with egg-attenuated chlamydia psittaci 6bc. 19734358601
glucose requirements of l cells infected with chlamydia psittaci. 19704394906
[particle membranes of the causative agent of ornithosis]. 19744439878
growth of chlamydia psittaci strain meningopneumonitis in mouse l cells cultivated in a defined medium in spinner cultures. 19724567659
[morphological manifestations of the intracellular toxic action of viruses]. 19744615467
detection of chlamydial antibodies in animal sera by double diffusion in gel.postinoculation sera collected from pigeons, turkeys, guinea pigs, sheep, a calf, a rabbit, and a horse experimentally infected with various strains of chlamydia psittaci yielded a high incidence of positive reactions when tested by double diffusion in gel. antigen was a deoxycholate extract of sa-2 strain of c. trachomatis. good correlation was obtained with results of complement fixation tests, whereas double diffusion in gel was less sensitive. immunoelectrophoresis of the antigen revealed pr ...19724622980
indirect hemagglutination test for chlamydial antibodies.an indirect hemagglutination (iha) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of chlamydia psittaci grown in vero cell monolayers were used. adaptation of the iha test to the microtiter system decreased sensitivity; nevertheless, the microtiter-iha test was more sensitive than the complement fixation test. lymphogranuloma venereum antibodies also were detected by using antigen extracte ...19724626906
detection of chlamydia psittaci by immunofluorescence.a direct fluorescent-antibody (fa) test was developed to detect chlamydia psittaci in dural impressions from specimen-inoculated mice. technical procedures for the test were compared. c. psittaci was found in mice after infection as early by the fa technique as it was by cytochemical staining methods usually used. the lymphogranuloma venereum organism was also stained by conjugated antibody to c. psittaci. a distinctive advantage of the described fa test is that organisms are identified immunolo ...19724626909
formation and destruction of internal membranes in l cells infected with chlamydia psittaci.l cells (mouse fibroblasts), uninfected and infected with chlamydia psittaci (meningopneumonitis strain), were labeled with (14)c-amino acids, and their membranous organelles were separated by isopycnic equilibrium centrifugation of whole cell homogenates on discontinuous sucrose density gradients. incorporation of labeled amino acids into host and parasitic proteins was differentiated on the basis of susceptibility to cycloheximide. twenty hours after infection with c. psittaci, incorporation o ...19734697788
miniature cell formation in chlamydia psittaci.in an electron microscope study on the developmental cycle of the goat pneumonitis strain of chlamydia psittaci in l cells, it was observed that miniature reticulate bodies, measuring approximately 0.2 mum in diameter and surrounded by double unit membranes, were produced infrequently from normal-sized reticulate bodies through a "budding"-like process. as little as 0.1 mug of penicillin per ml greatly increased the frequency of the miniature reticulate body formation.19734698212
[resistance of chlamydia psittaci against chlortetracycline]. 19734699553
natural intestinal infection with chlamydia psittaci in a closed bovine herd: serologic changes, incidence of shedding, antibiotic treatment of the herd, and biologic characteristics of the chlamydiae. 19734703505
pathogenicity of a strain of chlamydia psittaci of bovine intestinal origin for neonatal calves. 19734703506
effect of chloramphenicol, rifampicin, and nalidixic acid on chlamydia psittaci growing in l cells. 19734734704
[electron microscopic studies on morphological changes of chlamydia psittaci induced by a small dose of penicillin (author's transl)]. 19734808058
[the immune peroxidase method for demonstration of virus and chlamydia antigens. ii. demonstration of the evolutional circle of chlamydia psittaci in peritoneal macrophages of infected mice]. 19744824502
cycloheximide-resistant glycosylation in l cells infected with chlamydia psittaci.l cells (mouse fibroblasts), uninfected and infected with the meningopneumonitis strain of chlamydia psittaci, were labeled with [(14)c]glucosamine, and their membranous organelles were separated by isopycnic equilibrium centrifugation of whole cell homogenates on discontinuous sucrose gradients. glycosylation of host membranes continued throughout the infection. cycloheximide almost completely inhibited glycosylation in uninfected l cells, but it only partially inhibited the process in infected ...19744856239
hemagglutinin in cell walls of chlamydia psittaci.intact purified elementary bodies (eb) of chlamydia psittaci agglutinate chicken erythrocytes in low titer, whereas homogenates of eb and of eb cell walls agglutinate at much higher titers depending on the extent of disruption by shaking and sonication. the hemagglutinin is contained in the cell envelope and can be purified with cell wall fractions. treatment of cell wall with sodium dodecyl sulfate completely inactivated the hemagglutinin. purified hemagglutinin was found to have an identical p ...19744856587
[chlortetracycline content in feces from psittacines used for chlamydia psittaci isolation]. 19744856817
proteinase produced by chlamydia psittaci in l cells.l cells (mouse fibroblasts) infected with chlamydia psittaci (strain meningopneumonitis) produced a proteinase differing in solubility in ammonium sulfate from the proteinase of uninfected l cells. synthesis of the enzyme was inhibited by chloramphenicol but not by cycloheximide, indicating that the new proteinase in infected l cells was synthesized by chlamydia psittaci. the chlamydial proteinase had no demonstrable ion requirements and was not inhibited by a variety of inhibitors of proteinase ...19744857192
the toxicity of meningopneumonitis organisms (chlamydia psittaci) at different stages of development. 19694900518
demonstration of host-species-specific antigens in chlamydia psittaci by the plaque-reduction test in l-cell tissue cultures. preliminary observations. 19704920044
[ultrastructure and cytochemistry of chlamydia psittaci]. 19714939597
control of contamination in psittacosis specimens by antibiotics.diluent containing a combination of vancomycin, kanamycin, and streptomycin is shown to be more effective than diluent previously used for isolating chlamydia psittaci from bird tissue specimens.19714940879
lipid composition of chlamydia psittaci grown in monkey kidney cells in defined medium.the lipid compositions of (i) monkey kidney (mk-2) cells cultivated in eagle's minimal essential medium (mem) with 5% calf serum, (ii) mk-2 cells cultivated in waymouth medium supplemented with 20 mug of sodium oleate and 2 mg of bovine albumin per ml, (iii) chlamydia psittaci strain 6bc grown in the latter host system, and (iv) calf serum were compared. strain 6bc contains 31% phosphatidyl ethanolamine (pe) and 15% phosphatidyl glycerol (pg), whereas the host cell contains almost the same amoun ...19704987309
lipid synthesis by isolated chlamydia psittaci.the isolated cells of the host-dependent meningopneumonitis agent, chlamydia psittaci, were shown to incorporate radioactive carbon from aspartate, isoleucine, and glucose-6-phosphate into cell lipids. the nature of this incorporation was investigated. radioactivity was found only in the fatty acids and primarily in the phosphatidyl ethanolamine, and, to a lesser extent, in the phosphatidyl choline fractions. branched-chain fatty acids, not found in host lipid, were shown to constitute a large p ...19695354949
electron microscopic observations on the effects of penicillin on the morphology of chlamydia psittaci.l cells were infected at high multiplicity with meningopneumonitis organisms and incubated in medium containing 200 units per ml of penicillin. at intervals up to 48 hr after infection, cells were removed and thin sections were prepared for electron microscopic studies on the morphology of the developing organism. penicillin had no effect on the initial reorganization of the infecting elementary body to form the developmental reticulate body (rb), and, up to 12 hr after infection, the treated an ...19705413965
antibody for chlamydia psittaci in ascitic fluids of immunized mice implanted with sarcoma 180. 19705423527
[study with electron microscopy of the development and morphology of chlamydia psittaci in mouse macrophages]. 19705459084
cross immunity among strains of chlamydia psittaci. 19705486731
electron microscopic observations on the structure of the envelopes of mature elementary bodies and developmental reticulate forms of chlamydia psittaci.purified suspensions of chlamydia psittaci were prepared from l cells. thin sections of intact elementary bodies and intact developmental reticulate bodies and of their purified envelopes were observed by electron microscopy. in both intact organisms and partially purified envelopes, two membranous structures, each appearing in electron micrographs as two darkly stained layers, were observed. in the elementary body sections, the outer membrane was round, apparently rigid, and was not soluble in ...19715541020
effect of penicillin on the multiplication of meningopneumonitis organisms (chlamydia psittaci).although formation of infectious particles of meningopneumonitis organism in l cells was completely inhibited by 1 or more units of penicillin per ml, multiplication of reticulate bodies was observed, by light microscopy, in the presence of 200 units of penicillin per ml in stained smears of infected cells. when reticulate bodies were purified from cultures containing penicillin after 18, 30, and 45 hr of incubation, continuously increasing yields were obtained. when penicillin was added to infe ...19685686015
stability of the nucleic acids of l cells after infection with the meningopneumonitis agent.the stability of host nucleic acids in l cells infected with chlamydia psittaci (strain meningopneumonitis) was studied. the l cells were prelabeled with either (32)p-orthophosphate, (3)h-uridine, or (3)h-thymidine. after infection, the redistribution of each label among the different fractions of host and parasite was quantitatively determined and compared. there were no signs of accelerated degradation of host nucleic acid as the consequence of meningopneumonitis infection. comparison of the s ...19685724971
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