| enzyme-linked immunosorbent assay for the detection of chlamydial antigens from human specimens. | an assay for the determination of chlamydial antigens from human specimens is reported. the assay, called the rapid bead enzyme-linked immunosorbent assay (rb-elisa) is a relatively simple, inexpensive test. the rb-elisa for direct chlamydial determination can be adapted to either small or large-scale screening operations and could represent an additional detection method in conjunction with cell culture isolation techniques. | 1984 | 6374386 |
| [kinetic study of the antibodies in of 1 and c57bl mice infected with chlamydia trachomatis and chlamydia psittaci. its application to the interpretation of the serological results in human chlamydioses]. | the kinetics of antibodies to c. trachomatis and c. psittaci was studied in of 1 and inbred c 57 bl mice, inoculated intravenously (iv) or intraperitoneally. antibodies were detected by a micro-immunofluorescence (mif) test. antibody kinetics depends on many factors (genetic particularities of the mice, route of inoculation, chlamydial strain). the response is more specific when mice are inoculated with c. trachomatis than with c. psittaci; heterospecific antibodies appear later in c 57 bl mice ... | 1984 | 6377679 |
| enzyme immunoassay of chlamydia in birds. | serum samples from 192 free-living birds (27 species) were tested for antibodies against chlamydia using the enzyme-linked immunosorbent assay (elisa); 97 (51%) were seropositive. the highest antibody prevalence was among pheasants (96%), ducks (88%), and blackbirds (86%). none of 41 starlings tested were seropositive. the serotesting of 42 confined pheasants indicated 100% exposure to the organism. the elisa is a sensitive, rapid serologic method that can be of epidemiologic and diagnostic valu ... | 1984 | 6385949 |
| serological evidence of chlamydia trachomatis infection in non-immunocompromised adults with pneumonia. | serological evidence of chlamydia trachomatis infection in three non-immunocompromised adults with pneumonia is presented. complement fixation (cf) tests indicated chlamydial infections in all three patients. these illnesses would have been erroneously regarded as ornithosis if microimmunofluorescence (micro-if) tests had not been performed because the cf test detects genus-specific antibodies while the micro-if test reveals species-specific antibodies. | 1984 | 6389713 |
| [is chlamydia psittaci a complicating factor in paramyxovirus 1 infection in pigeons?]. | | 1984 | 6397343 |
| differential amino acid utilization by chlamydia psittaci (strain guinea pig inclusion conjunctivitis) and its regulatory effect on chlamydial growth. | the effect of omission of individual amino acids from growth medium on the multiplication of chlamydia psittaci (strain guinea pig inclusion conjunctivitis) in cycloheximide-treated mccoy cells has been examined. marked differences were observed in the amounts of particular amino acids required for normal chlamydial multiplication: omission of either leucine, phenylalanine or valine completely inhibited multiplication, whereas absence of any one of another 10 amino acids had no effect on numbers ... | 1983 | 6415225 |
| lymphokine-mediated inhibition of chlamydia replication in mouse fibroblasts is neutralized by anti-gamma interferon immunoglobulin. | experiments were carried out to characterize immunologically mediated chlamydial persistence in cell culture. mouse fibroblasts were activated to restrict chlamydia psittaci 6bc replication by including mitogen (concanavalin a)-induced spleen cell supernatant fluids from immunized animals in the growth medium. when mouse fibroblasts were incubated with lymphokine for 24 h before infection and then with growth medium after infection (preinfection treatment), chlamydial replication was delayed but ... | 1983 | 6417024 |
| [avian chlamydiosis. (1) imported birds]. | | 1983 | 6418836 |
| [respiratory tract infections caused by viruses, mycoplasma, rickettsia and chlamydia with bronchial and pulmonary localization]. | | 1984 | 6424057 |
| monoclonal antibody against a genus-specific antigen of chlamydia species: location of the epitope on chlamydial lipopolysaccharide. | monoclonal antibodies were prepared by the fusion of murine myeloma ns1 cells with spleen cells of balb/c mice immunized with formalin-killed elementary bodies of the chlamydia trachomatis l2 serovar. the specificity of these monoclonal antibodies was determined with a solid-phase immunoassay in which hela 229 cells infected with c. trachomatis serovars d, g, h, i, l2 and the chlamydia psittaci meningopneumonitis strain cal-10 were used. an immunoglobulin g3 monoclonal antibody (l2i-6) was ident ... | 1984 | 6425219 |
| [evaluation of the incidence of serological cross reactions between legionella and mycoplasma or chlamydia]. | because of the possible serological cross reactions frequently described in the literature between l. pneumophila and m. pneumoniae or between l. pneumophila and chlamydia, we decided to determine their frequency with our sera and our own methods. the anti m. pneumoniae and chlamydia antibodies were researched in 162 sera samples from 46 cases of legionellosis serologically diagnosed and in 51 sera from 26 hyperimmunised rabbits. conversely, anti l. pneumophila antibodies were researched in 165 ... | 1984 | 6425788 |
| [isolation of chlamydia psittaci from an aborted bovine fetus]. | one strain of chl. psittaci was isolated from two aborted foetuses of two cows coming from the same locality. tissue from the lungs of the aborted foetuses was used for the isolation tests; after sample preparation this tissue was inoculated into the yolk sacs of chick embryos. it was found on the basis of serological examination (rvk) that both aborting cows had positive levels of antibodies against the chl. psittaci antigen at a serum dilution ratio of 1 : 64. the serological examination for b ... | 1984 | 6426125 |
| [serologic and clinical studies of chlamydial infection in children with respiratory tract infection]. | | 1984 | 6434643 |
| a comparison of the efficacy of a live and four inactivated vaccine preparations for the protection of cats against experimental challenge with chlamydia psittaci. | a commercial live feline-chlamydial vaccine and four experimental inactivated preparations were compared on the basis of clinical protection in cats challenged conjunctivally and intranasally with chlamydia psittaci. best protection was afforded by the live vaccine. good results were also obtained using inactivated preparations of a recent feline conjunctival isolate. protection did not correlate with the development of complement fixing antibodies but may be related to the induction of a cell m ... | 1980 | 6448682 |
| [chlamydial infection in mass abortions in goats]. | etiologic and clinical-and-epizootiologic studies were carried out of a chlamydial infection in goat flocks where abortions took place on a mass scale. discovered were two foci of chlamydial abortion. two strains of chlamydia psittaci were isolated from aborted fetuses and from placentas on two farms. the strains were identified both antigenically and morphologically. the serologic investigation of a total of 167 goats on 6 farms with abortions revealed that in the course of two enzootics as man ... | 1984 | 6474822 |
| cutaneous findings in a case of psittacosis. | a patient had severe pneumonia, respiratory failure, and disseminated intravascular coagulation caused by chlamydia psittaci. cutaneous findings included a maculopapular blanching rash, acrocyanosis, superficial venous thromboses, and splinter hemorrhages under the fingernails. the history of exposure to potential vectors of psittacosis and the presence of some or all the cutaneous findings described herein may lead to the early recognition of psittacosis. | 1984 | 6476861 |
| growth of several strains of chlamydia psittaci in vero and mccoy cells in the presence of cytochalasin and cortisone. | chlamydia psittaci of avian and mammalian origin were grown in mccoy and vero cells in the presence of cytochalasin b with cortisone acetate. six turkey strains, one parrot strain, one human strain, one ovine strain and one bovine strain were used in this study. all strains, except the turkey strain nj-1, grew to higher titers in vero cells than in mccoy cells. five of the six turkey strains could be grouped together because they induced cytopathic changes rapidly. the other turkey strain, along ... | 1984 | 6478298 |
| observations on a skin sensitivity test for ovine enzootic abortion. | | 1984 | 6487996 |
| chlamydia psittaci experimental abortion in goats. | to study abortive chlamydiosis in goats, 11 pregnant goats were inoculated intradermally at the 3rd month of pregnancy with 2 x 10(7) or 2 x 10(6) plaque-forming units of a strain of chlamydia psittaci isolated from naturally occurring abortions in goats. the 11 inoculated does aborted 24 to 56 days after they were inoculated and shed chlamydia. this shedding began at least 9 days before abortion (1 goat) and persisted 12 days after abortion (1 goat). retained placenta or metritis were observed ... | 1984 | 6497108 |
| spontaneous abortion--an infectious aetiology? | the role of chlamydia trachomatis, genital mycoplasmas, campylobacter spp. and other aerobic and anaerobic bacteria in the aetiology of spontaneous abortion was investigated prospectively in 241 pregnant women at a community hospital. sixteen women who had threatened abortions were a little younger, of lower social class and had had more previous spontaneous abortions than the 76 women who aborted or the 149 women whose pregnancies were not complicated in the early stages by haemorrhage. the dem ... | 1984 | 6518152 |
| prevalence of chlamydia psittaci in imported psittacine birds from 1981 to 1983. | | 1984 | 6521156 |
| incidence of chlamydial antibodies in commercial duck flocks. | | 1984 | 6523715 |
| [identification of the kaunas-1 strain isolated in the lithuanian ssr in enteritis in calves]. | | 1984 | 6528580 |
| [psittacosis. study of 2 cases occurring in a family]. | | 1984 | 6531486 |
| [chlamydial infection in herds of cattle with abortions]. | a study was carried out for the presence of chlamydia on 103 farms with records of abortions. employed were methods of infecting chick embryos via the yolk sac, the complement-fixation test, and electron microscopy. five chlamydia strains were isolated from aborted fetuses on 5 out of 11 studied farms. statistical processing of the serologic results obtained revealed that on 20 farms up to 71 per cent of the cows that miscarried were serologically positive for chlamydia, showing titers within th ... | 1984 | 6531862 |
| preparation of complement fixation antigen of chlamydia psittaci grown in tissue culture by treatment with beta-propiolactone. | a new method of preparing a chlamydial complement fixation (cf) antigen by treatment with beta-propiolactone (bpl) is presented. chlamydia psittaci strains pigeon-1041 and budgerigar-no. 1, and chlamydia trachomatis strain l2/434/bu, propagated in l-929 cell monolayers, were inactivated with bpl. this bpl-treated antigen was useful for detecting cf antibodies in both human and pigeon sera, and it did not cause false-positive reactions, as are sometimes observed between some human sera and phenol ... | 1984 | 6533439 |
| isolation of chlamydia psittaci from koalas (phascolarctos cinereus). | | 1984 | 6534365 |
| [pulmonitis caused by coxiella burneti and chlamydia psittaci. 2 years of research in marche]. | this paper point to infections caused by chlamydia psittaci and coxiella burneti in a region of the middle italy (marche) from april 1981 to march 1983. 32 of 686 cases (about 5%) of pneumonia were diagnosed as q fever or ornithosis-psittacosis. the role of the research of these etiologies in routine diagnostic work on acute respiratory infections is underlined. | 1984 | 6534399 |
| [rickettsiosis and chlamydiosis in hoggar (republic of algeria): epidemiological sampling]. | the study was made on reservoirs of infections (rodent, sheep, goat, dromedary), vectors (louse) and human-receptors. examinations were made on smears examined with staining and indirect-fluorescente techniques and on sera examined by complement fixation test. chlamydia psittaci appears very often in rodent, sheep, goat, dromedary and man. antibodies against coxiella burneti were found present in sheep, goat but mainly in dromedary and less than 16 years old boys. two lice were carrier of ricket ... | 1984 | 6548418 |
| update on chlamydiosis. | | 1984 | 6610968 |
| [method of testing chemical disinfectants for their effectiveness against chlamydia]. | | 1983 | 6613415 |
| [experimental infection of the pregnant cow with chlamydia psittaci]. | seven cows were inoculated with two strains of c. psittaci from bovine origin. their chlamydial excretion, complement fixing (cf) antibody titer and hormonal pattern, at calving or abortion were monitored. five of the six pregnant inoculated cows had a pathological parturition, and a cf antibody titer greater than 40 during more than two months post-abortion. three of them excreted chlamydiae at calving. the other inoculated pregnant cow, the inoculated non pregnant cow and the three contacts ha ... | 1983 | 6614789 |
| response of ewes to temperature-sensitive mutants of chlamydia psittaci (var ovis) obtained by ntg mutagenesis. | two temperature-sensitive strains, 1b and 1h, of ovine chlamydia psittaci obtained by mutagenesis were used as live vaccine: eleven weeks before breeding, 20 ewes received 3.9 x 10(6) pfu of 1b strain, and 20 others 3.5 x 10(6) pfu of 1h strain. the consequences of the vaccination were studied during pregnancy by recording cf antibody titer, chlamydial vaginal excretion and lambing performance. the vaccination did not disturb pregnancy and none of the ewes excreted chlamydiae at lambing. the imm ... | 1983 | 6614792 |
| amino acid requirements of strains of chlamydia trachomatis and c. psittaci growing in mccoy cells: relationship with clinical syndrome and host origin. | the effects of omission of individual amino acids from growth medium on the multiplication of a range of chlamydia trachomatis and c. psittaci strains in cycloheximide-treated mccoy cells have been assessed. differences in requirements were revealed which for c. trachomatis strains correlated with clinical syndrome and for c. psittaci with host origin. all 11 strains of c. trachomatis examined showed a requirement for addition of histidine to the medium; this was not shown by any of four c. psit ... | 1983 | 6631408 |
| in vitro and in vivo properties of chemically induced temperature-sensitive mutants of chlamydia psittaci var. ovis: screening in a murine model. | two temperature-sensitive (ts) strains, 1b and 1h, were obtained after nitrosoguanidine treatment of the wild-type virulent abortive ovine strain chlamydia psittaci ab7. optimum growth temperature on mccoy cells was 38 degrees c for the three strains, but at the restrictive temperature, 39.5 degrees c, ts strains differed from the parental strain in their total infective yield of chlamydiae, their efficiency of plating, and the morphology of the plaques and cytoplasmic inclusions. their survival ... | 1983 | 6642642 |
| carbohydrate inhibition of chlamydial infectivity. | the effects of exogenous carbohydrates on chlamydial infectivity were tested. it was shown that the addition of certain carbohydrates reduced the infectivity of chlamydia psittaci in cell culture. the greatest inhibitory effects were observed by monosaccharides, disaccharides and trisaccharides, respectively. the addition of atp, but not amp, enhanced the inhibitory effect. | 1983 | 6645980 |
| chlamydiosis in collared doves. | | 1983 | 6649351 |
| direct isolation of the agent of enzootic abortion of ewes (chlamydia psittaci) in cell cultures. | isolation of chlamydia psittaci in mccoy cell coverslip cultures from clinical material from field cases of enzootic abortion of ewes proved more sensitive than diagnosis by examination of smears stained by ziehl-neelsen. enzootic abortion could be diagnosed in the absence of fetal membranes by culture of fetal lung or liver tissue. | 1983 | 6649373 |
| abortion and stillbirth associated with chlamydia psittaci var ovis in dairy goats with high titers to toxoplasma gondii. | during two consecutive breeding seasons in a brucellosis-free dairy goat herd, approximately 40% to 70% of the does (mean herd size, 14.5) had stillbirths or aborted. none of the does aborted or had stillbirths twice. in the first breeding season, 9 of 13 does aborted in the last 2 months of gestation. three fetuses and 2 fetal placentas from 2 does were examined. microscopic findings included severe multifocal hepatic necrosis, mild pneumonia, and mild multifocal necrosis in the brain. cultures ... | 1983 | 6654724 |
| [serological study of chlamydial abortion in sheep]. | a vast serologic study of the chlamydial abortion in sheep was carried out. the complement-fixation test was employed to investigate a total of 3808 ewes--both animals that had miscarried and various groups of controls. the results obtained were processed statistically. forty-four new foci of chlamydial abortion in sheep were discovered. it was shown that the serologic diagnosis of the disease was possible with the study of serum samples from animals with abortions, taken once and twice. the art ... | 1983 | 6666022 |
| prevalence of chlamydia psittaci in pet birds maintained in public places or in close human contact. | | 1983 | 6672412 |
| isolation and serological survey of chlamydia psittaci in feral pigeons from japan. | | 1983 | 6672413 |
| [evaluation of the incidence of ornithosis based on serological studies]. | | 1983 | 6676724 |
| structural and polypeptide differences between envelopes of infective and reproductive life cycle forms of chlamydia spp. | significant differences in cysteine-containing proteins and detergent-related solubility properties were observed between outer membrane protein complexes of reproductive (reticulate body) and infective (elementary body) forms of chlamydia psittaci (6bc). elementary bodies harvested at 48 h postinfection possessed a 40-kilodalton major outer membrane protein and three extraordinarily cysteine-rich outer membrane proteins of 62, 59, and 12 kilodaltons, all of which were not solubilized by sodium ... | 1984 | 6690419 |
| psittacosis/ornithosis in cambridgeshire 1975-1983. | in a population of approximately 300 000 there were 150 illnesses attributable to chlamydial infection from january 1975 to june 1983 and nearly all were presumed due to chlamydia psittaci. diagnosis was based on the complement-fixation (cf) test, the disadvantages of which are discussed, especially anamnestic responses. there were 73 cases of pneumonia, 37 febrile respiratory infections, 35 miscellaneous conditions and five asymptomatic seropositive contacts. the annual number of cases was lowe ... | 1984 | 6693765 |
| vaccination with temperature-sensitive mutant of chlamydia psittaci against enzootic abortion of ewes. | | 1984 | 6710835 |
| isolation of chlamydia psittaci from newly imported keas (nestor notabilis). | | 1984 | 6719778 |
| vaccination against enzootic abortion in ewes. | | 1984 | 6719783 |
| isolation of chlamydia psittaci from nasal and conjunctival exudate of a domestic cat. | a feline strain of chlamydia psittaci was isolated in tissue culture from nasal and conjunctival swabs from a free range domestic cat with bilateral conjunctivitis and rhinitis, living in the liverpool area of the uk. the isolate was identified as c psittaci on the basis of its characteristic inclusion morphology in cell culture and by means of specific indirect immunofluorescence with known c psittaci specific antiserum. the isolate could be differentiated from other chlamydiae of non-feline or ... | 1984 | 6719789 |
| isolation of chlamydia psittaci from cases of conjunctivitis in a colony of cats. | this report describes the isolation in cell cultures of chlamydia psittaci from cases of conjunctivitis in a colony of cats. the organism was identified in mccoy cell monolayers by staining the intracytoplasmic chlamydial inclusions with a fluorescent antibody technique, and serological evidence of chlamydial infection in cats was obtained by indirect immunofluorescence. the possible role of c psittaci as an ocular, upper respiratory and reproductive tract pathogen in cats is discussed. | 1984 | 6719790 |
| growth of chlamydia in pig lung alveolar macrophages; preparation of macrophages and demonstration of growth. | the harvesting, storing and utilisation in tissue culture of lung alveolar macrophages from 2-3 weeks old piglets were investigated. an average yield of 4 x 10(8) cells was obtained, which in culture usually remained healthy for at least 8 days. in liquid nitrogen at -173 degrees c about 50% of the cells were still viable after 3 months. pig cells sustained the growth of ovine chlamydia psittaci and a rising titre was demonstrated over 4 passages. | 1984 | 6719825 |
| [comparative studies of psittacosis control on a drug basis. ii. efficacy trial of different drugs in different dosage forms in experimentally infected parrots (amazona viridigenalis)]. | | 1984 | 6732739 |
| elementary body envelopes from chlamydia psittaci can induce immediate cytotoxicity in resident mouse macrophages and l-cells. | isolated, purified chlamydia psittaci elementary body envelopes at a high multiplicity of infection (1,000:1) are capable of inducing immediate cytotoxicity in resident mouse macrophages and 929 l-cells. | 1984 | 6735471 |
| epidemiologic and laboratory observations of chlamydia psittaci infection in pet birds. | psittacosis was diagnosed in 13 (24%) of 55 pet bird submissions in the first 3 months of 1983, compared with 18 (17%) of 103 in 1982, 4 (4.6%) of 87 in 1981, and 3 (8%) of 36 in 1980. the numbers and types of birds in which psittacosis was diagnosed were 2 of 47 budgerigars, 8 of 45 cockatiels , 3 of 11 small parrots ( lovebirds , conures ), 20 of 116 medium-sized parrots (african grays, amazons ), 2 of 21 macaws , and 3 of 21 cockatoos . thirty-nine percent of psittacosis-positive birds were r ... | 1984 | 6735859 |
| an outbreak of psittacosis in a boys' boarding school. | in 1980, an outbreak of a mild illness involving twenty pupils and four adults in a boys' boarding school was identified serologically as psittacosis. intensive epidemiological investigations did not uncover the source. it is suggested either that sources of chlamydia other than avian exist, which may produce a milder illness than the avian type, or that human-to-human spread of a mild form of chlamydiosis occurs. | 1984 | 6736642 |
| experimental chlamydial pneumonia in pigs. | naturally occurring disease in pigs associated with chlamydial infections has not been reported in britain, though evidence of chlamydial challenge has been demonstrated in two separate serological surveys. an isolate of chlamydia psittaci (28/68) from an ovine pneumonia produced pneumonia in pigs following intratracheal inoculation. transient pyrexia at 24 hr was followed by increased respiratory rates and inappetance which lasted for a further 48 hr in challenged pigs. histologically acute exu ... | 1984 | 6744810 |
| isolation and serological survey of chlamydiosis in feral pigeons and crows in hokkaido. | | 1984 | 6748450 |
| serotyping of chlamydia psittaci by the micro-immunofluorescence test: isolates of ovine origin. | the micro-immunofluorescence test was successfully applied to seven ovine isolates which could be separated into two distinct immunotypes. the pattern of reactivity was similar to that elucidated by the plaque reduction test and confirmed by growth characteristics and biotyping. | 1982 | 6752025 |
| [comparison of 2 immunofluorescent technics for detection of anti-chlamydia antibodies]. | | 1982 | 6759508 |
| [experimental study of an antibody sepharose diagnostic reagent for ornithosis]. | | 1982 | 6760554 |
| embolic phenomena in chlamydial infection. | | 1980 | 6764481 |
| [serologic diagnosis of human ornitho-psittacosis. value of the indirect immunofluorescent test]. | | 1982 | 6765105 |
| [isolation of chlamydia psittaci from bull ejaculate]. | during the repeated serological examination (rvk) in five breeding bulls the positive levels of antibodies to chlamydia psittaci in titre 1 : 128 were found. in the isolation experiments the pelleted ejaculates deposited in liquid nitrogen were used. the isolation of chlamydia psittaci on yolk sacs of chicken embryos was positive in two breeding bulls. the isolated strains are labelled gn-33 and ok-107. the serological examination of blood samples was in all five breeding bulls negative on bruce ... | 1980 | 6777949 |
| [epidemiology and diagnosis of legionella infections in austria (author's transl)]. | out of 2,105 patients with atypical pneumonia and febrile infections 15 cases of legionellosis were diagnosed by the indirect immunofluorescent antibody test (ifa) in austria from the middle of 1977 to the end of 1979. among the patients with the diagnosis of atypical pneumonia legionnaires' disease was found in 0.65%. among those patients whose sera were examined because of suspected legionella infection the frequency was 1.96% (p less than 0.1). therefore it may assumed that some symptoms of l ... | 1981 | 6791407 |
| [preparation of cf and ha antigens for serological diagnosis of chlamydia psittacosis and serological survey among inhabitants in aichi prefecture (author's transl)]. | | 1981 | 6799592 |
| pregnancy complicated by psittacosis acquired from sheep. | two cases of chlamydial infection in pregnant women are described, the first serologically proved and the second suspected. in both cases the infection was probably contracted from sheep suffering with enzootic abortion. both patients were farmers' wives who had helped their husbands and lambing and developed a non-specific febrile illness in late pregnancy. in the first case as there was no clinical improvement after 26 hours the patient was delivered by caesarean section of a live infant in go ... | 1982 | 6803874 |
| [pathogenicity of chlamydia. findings in human medicine (author's transl)]. | | 1982 | 6804856 |
| animal models for the study of chlamydial infections of the urogenital tract. | various experimental animal models have been used for the study of chlamydial infections of the urogenital tract. experimental urethral and vaginal inoculation of guinea pigs with the guinea-pig inclusion conjunctivitis agent provoke urogenital infection. direct inoculation of this agent into the uterine horns of guinea pigs induced an acute self-limiting salpingitis. in cats, urethritis and vaginitis have been produced with the feline keratoconjunctivitis agent. the results obtained by using th ... | 1982 | 6813962 |
| [isolation of chlamydia psittaci and moraxella bovis from infectious keratoconjunctivitis in lambs]. | out of 189 lambs in the flock, 25 animals suffered from bilateral or unilateral conjunctivitis, or keratoconjunctivitis. by serological examination (rvk), positive levels of antibodies to the group-specific antigen of chl. psittaci were found in three out of six lambs examined by laboratory methods. bacteriological examination of eye smears of six lambs showed in four cases the infection by microorganisms of moraxella bovis. smears from the conjunctivas of these lambs were after preparation inst ... | 1982 | 6814042 |
| viral and mycoplasmal antibodies in patients with myocardial infarction. | the suggestion that infection might provoke myocardial infarction prompted a controlled study among 49 patients with myocardial infarction for antibody titres to 22 viruses, and to mycoplasma pneumoniae and chlamydia psittaci. the frequency of significant rises in antibody titres was similar in patients with myocardial infarction, 7 (14%) out of 49, and in control patients, 8 (22%) out of 37. thus, in our limited series we could not confirm the hypothesis that viral or mycoplasmal infection are ... | 1981 | 6814338 |
| differential susceptibility of chlamydiae to exogenous fibroblast interferon. | mouse fibroblasts (l cells) were incubated for 5 h with 1,000 u of murine fibroblast interferon (muifn alpha+ beta) per ml and then were infected with either chlamydia trachomatis (lgv 440), c. psittaci (6bc), or c. psittaci (cal 10). intracellular development of c. trachomatis was reduced 90% in interferon-treated cells 24 h after infection when compared with controls, whereas c. psittaci growth was not affected in interferon-treated cells. | 1983 | 6832807 |
| inhibition of onset of overt multiplication of chlamydia psittaci in persistently infected mouse fibroblasts (l cells). | when monolayers of mouse fibroblasts (l cells) persistently infected with chlamydia psittaci (strain 6bc) were dispersed in medium 199 and plated out in new flasks, the monolayers that grew out consisted almost exclusively of inclusion-free host cells that retained full resistance to superinfection with c. psittaci (covert infection). after a delay that was inversely proportional to the initial density of the newly transferred l cell population, the percentage of host cells containing visible ch ... | 1983 | 6832822 |
| multiantibiotic resistance in chlamydia psittaci from ducks. | | 1983 | 6836912 |
| chlamydiosis. | | 1983 | 6839126 |
| inhibition of chlamydia psittaci in oxidatively active thioglycolate-elicited macrophages: distinction between lymphokine-mediated oxygen-dependent and oxygen-independent macrophage activation. | immune sensitization of spleen cells was required to generate lymphokines (lk) that activated thioglycolate-elicited peritoneal macrophages (thio macs) to respond via both oxygen-dependent and oxygen-independent systems. lk produced by incubating spleen cells from immunized a/j and laf mice with concanavalin a stimulated a response by thio macs to phorbol-12-myristate-13-acetate (pma)-induced chemiluminescence and activated these cells to inhibit intracellular chlamydia psittaci replication. con ... | 1983 | 6840848 |
| chlamydia psittaci elementary body envelopes: ingestion and inhibition of phagolysosome fusion. | the cell surface of chlamydia psittaci seems important for establishing infection since (i) uv-treated elementary bodies (eb) attach to and are ingested by l cells and (ii) heat or antibody treatment decreases attachment to l cells and promotes the fusion of chlamydiae-containing phagosomes with lysosomes in macrophages. in the studies reported here, [3h]uridine-labeled uv-treated eb also persisted in mouse resident peritoneal macrophages and l cells, suggesting that phagosome-lysosome fusion is ... | 1983 | 6840860 |
| disulfide-linked oligomers of the major outer membrane protein of chlamydiae. | the major outer membrane protein of chlamydial elementary bodies was identified in dimer, trimer, and other multimeric forms. these natural multimers were stabilized by disulfide-mediated cross-linking. such cross-linking of outer membrane proteins may play an important role in the formation and evolution of chlamydial cell wall structure. | 1983 | 6841322 |
| laboratory transfer of a virus between isolates of chlamydia psittaci. | | 1983 | 6845606 |
| a mouse model to compare virulence of abortive and intestinal ovine strains of chlamydia psittaci: influence of the route of inoculation. | strains of chlamydia psittaci isolated from faeces of a clinically healthy ewe (intestinal strain ib1) and from the foetus of an aborted ewe (abortive strain ab7) were injected into pregnant mice via the intraperitoneal route and into non-pregnant mice via the footpad route. in pregnant mice the response to inoculation was followed by recording the performance and viability of infant mice, and in the non-pregnant mice by an enlargement of the spleen and colonization of both the spleen and poplit ... | 1983 | 6847040 |
| isolation and characterization of macrophage phagosomes containing infectious and heat-inactivated chlamydia psittaci: two phagosomes with different intracellular behaviors. | infectious chlamydia psittaci enters macrophages via a cytochalasin b-insensitive pathway in which chlamydia-containing phagosomes do not fuse with lysosomes; heat-inactivated c. psittaci enters macrophages via a route in which phagosomes do fuse with lysosomes. in an attempt to explain these differences, phagosomes containing infectious and heated chlamydiae were isolated from mouse macrophages by a procedure developed to isolate l-cell chlamydial phagosomes by rate zonal centrifugation. macrop ... | 1983 | 6852926 |
| lymphokine enhances oxygen-independent activity against intracellular pathogens. | to determine if mechanisms other than the generation of toxic oxygen intermediates are active against intracellular pathogens, oxidatively deficient mouse l cells and monocyte-derived macrophages from patients with chronic granulomatous disease were stimulated with soluble lymphocyte products. despite no enhancement in oxidative activity, these cells displayed effective microbistatic activity against both t. gondii and c. psittaci. these results suggest a potential role for nonoxidative mechanis ... | 1983 | 6864162 |
| [a method of production of concentrated and purified vaccine against chlamydia abortion in sheep and its testing in guinea pigs]. | a method was developed for the production of a new, present day, concentrated depot-vaccine against the chlamydial abortion of sheep. employed were a number of methods -- those with chick embryos, freezing and thawing with the use of liquid nitrogen, inactivation with formalin, purifying and concentration through differential centrifugation and supercentrifugation, with the use of an aluminum hydroxide ingredient, electron microscopy, and the respective control series, etc. the vaccine is charac ... | 1983 | 6868364 |
| lymphokine stimulated macrophages inhibit intracellular chlamydia psittaci replication by mechanisms distinct from intracellular inhibition of toxoplasma gondii replication. | | 1983 | 6869092 |
| [susceptibility of the macrophages of inbred strain mice to chlamydia psittaci]. | chlamydia psittaci was injected in the peritoneal cavity of different strains of inbred mice. not all of them develop a clinical disease; some, such as c 3 h, are very susceptible and died within 6 days. c 57 bl, however, are resistant even to a very high dose. we still do not know the exact mechanism of the natural resistance. this is the reason why we are trying to analyse the role of the macrophage. this cell is particularly important in c. psittaci infection, because it harbours the obligate ... | 1983 | 6871493 |
| susceptibility of chlamydia psittaci (ovis) to antimicrobial agents. | minimum inhibitory (mic) and minimal lethal (mlc) concentrations of 30 antimicrobial agents were determined for field strains and for the a22 eae vaccine strain of chlamydia psittaci (ovis). no differences in susceptibility could be detected between strains. the antimicrobials could be divided into four groups. group a had no effect on growth and development, group b were bacteriostatic at concentrations in excess of 100 mg/l, group c were bacteriostatic at concentrations of 10 mg/l or less but ... | 1983 | 6874628 |
| isolation and identification of chlamydia psittaci from collared doves (streptopelia decaocto). | | 1983 | 6879973 |
| infection control update! chlamydia. | | 1981 | 6907075 |
| interaction of chlamydiae with host cells and mucous surfaces. | for chlamydiae, as obligate intracellular parasites, attachment to and ingestion by host cells are essential steps in reproduction. their attachment site appears to be heat-sensitive; it has not been correlated with any morphological entity. antibody blocks chlamydial attachment to cells and, for certain chlamydia psittaci and chlamydia trachomatis strains which are highly infective for cell cultures, n-acetylglucosamine appears to contribute to cell receptor specificity. sialic acid residues ha ... | 1981 | 6911075 |
| [chlamydia respiratory infections]. | | 1982 | 6925319 |
| biology of chlamydia. | the genus chlamydia consists of two species, chlamydia trachomatis and c. psittaci. the former includes (a) the trachoma/inclusion conjunctivitis (tric) agents, subdivided into the serotypes a-k; (b) the lymphogranuloma venereum (lgv) agents, subdivided into the serogroups (l 1-3); and (c) the mouse penumonitis agent. the major characteristics differentiating the two species are sulfonamide susceptibility and the formation of distinct inclusion granula in host cells, in the case of c. trachomati ... | 1982 | 6958006 |
| unusual manifestations of chlamydia trachomatis infections. | systemic infections with chlamydia trachomatis are known to occur with the agents of lymphogranuloma venereum but are not generally recognized to occur with the trachoma and inclusion conjunctivitis (tric) agents, i.e., immunotypes a-k. the clinical spectrum of tric agent infections has expanded, however, and now includes deep-seated genital infections such as epididymitis and salpingitis, as well as infections in neonates. endocarditis, pneumonia in adults, otitis media, choroiditis and erythem ... | 1982 | 6958008 |
| infectivity of penicillin-induced chlamydia psittaci forms for cell cultures from the chorioallantoic membrane of chick embryos. | l-cells were infected at high multiplicity with meningopneumonitis organsims (mp) and incubated in medium with penicillin (200 units/ml) and without the drug. at intervals up to 36 hours cells were removed and l-cell associated chlamydial infectivity was determined for cell cultures from the chorioallantoic membrane (cam) of chick embryos which were treated before inoculation with diethylaminoethyl (deae)-dextran. in control l-cells, the infectivity decreased at 8 hours and reached it lowest lev ... | 1980 | 6999778 |
| chlamydiae. | | 1980 | 7002026 |
| [survival capacity of chlamydiae and coxiella burnetii under environmental conditions (author's transl)]. | | 1980 | 7006982 |
| [current knowledge concerning pathogenicity of mammalian chlamydial strains for humans]. | | 1980 | 7008768 |
| chlamydial antigens stabilized with formalin for use in the micro-immunofluorescence test. | formalinized antigen suspensions prepared by differential centrifugation from crude infected yolk sacs and stored at 4 degrees c were satisfactory antigens during at least 36 weeks when used in chlamydial micro-immunofluorescence procedures. | 1980 | 7012175 |
| inhibition of phagolysosome fusion is localized to chlamydia psittaci-laden vacuoles. | intracellular survival of chlamydia psittaci is in part dependent on the ability of the organism to thwart phagolysosome formation. circumvention of phagolysosome fusion could be either localized to chlamydia-laden vacuoles or generalized to all phagosomes in the host cell. to determine which of these modes is in operation the ability of chlamydia elementary and reticulate bodies to protect saccharomyces cerevisiae from degradation in macrophage phagolysosomes was examined via acridine orange an ... | 1981 | 7019080 |
| a modified isolation technique for chlamydia psittaci in l-cells treated with cycloheximide and glucocorticoid. | various auxiliary treatments of l-cells employed for the isolation and cultivation of c. psittaci were investigated in order to develop an improved method for the detection of the agent, in addition to the aid obtained by centrifugation and cycloheximide treatment. glucocorticoid treatment increased the observed number of inclusions considerably through a preservative effect on host cells and enhanced an spontaneous re-infection. besides, the hormone made the scanning of cell culture for inclusi ... | 1981 | 7020334 |
| [serotyping of "chlamydia psittaci" by microimmunofluorescence test (author's transl)]. | antisera from inbred mice (c57bl and c3h) improve the specificity of the microimmunofluorescence test. we used this method in the serotyping of 6 strains of chlamydia psittaci of differing origin. inspite of relatively marked cross-reactions it was possible to distinguish 3 groups: - the first group consisted of 2 strains of avian origin and the lgv strain 33l, - the second group consisted of 2 strains responsible for enzootic abortion in ewes, - in the third group, only 1 strain was found and t ... | 1981 | 7020525 |
| c. psittaci 6 bc soluble hemagglutinin: factors influencing the red cell receptor sites. | human blood-group a, b and o erythrocytes did not possess receptor sites for either crude or ether-ethanol extracted chlamydial soluble hemagglutinin. sensitive chicken erythrocytes were agglutinated to higher titres by ether-ethanol extracted than by crude chlamydial hemagglutinin. studies indicated that trypsin-, chymotrypsin-, neuramanidase-sensitive receptor sites were not essential for binding of ether-ethanol extracted chlamydial hemagglutinin; neither were beta-glucuronidase- nor periodat ... | 1981 | 7023129 |