| genomics of iron acquisition in the plant pathogen erwinia amylovora: insights in the biosynthetic pathway of the siderophore desferrioxamine e. | genomics has clarified the biosynthetic pathway for desferrioxamine e critical for iron acquisition in the enterobacterial fire blight pathogen erwinia amylovora. evidence for each of the individual steps and the role of desferrioxamine e biosynthesis in pathogen virulence and cell protection from host defenses is presented. using comparative genomics, it can be concluded that desferrioxamine biosynthesis is ancestral within the genera erwinia and pantoea. | 2011 | 21814817 |
| molecular and physiological properties of bacteriophages from north america and germany affecting the fire blight pathogen erwinia amylovora. | for possible control of fire blight affecting apple and pear trees, we characterized erwinia amylovora phages from north america and germany. the genome size determined by electron microscopy (em) was confirmed by sequence data and major coat proteins were identified from gel bands by mass spectroscopy. by their morphology from em data, fea1h and fea100 were assigned to the podoviridae and fea104 and fea116 to the myoviridae. host ranges were essentially confined to e. amylovora, strains of the ... | 2011 | 21791029 |
| metabolic versatility and antibacterial metabolite biosynthesis are distinguishing genomic features of the fire blight antagonist pantoea vagans c9-1. | pantoea vagans is a commercialized biological control agent used against the pome fruit bacterial disease fire blight, caused by erwinia amylovora. compared to other biocontrol agents, relatively little is currently known regarding pantoea genetics. better understanding of antagonist mechanisms of action and ecological fitness is critical to improving efficacy. | 2011 | 21789243 |
| anti-lipopolysaccharide factor evokes indirect killing of virulent bacteria in kuruma prawn. | aims: anti-lipopolysaccharide factor (alf) is an antimicrobial peptide (amp) and a key effector molecule of the innate immune system in crustaceans. however, little is known about the role of its indirect killing against bacteria. the possible regulatory role of this peptide (m-alf) in kuruma prawns, marsupenaeus japonicus, was investigated. | 2011 | 21753127 |
| pantoea stewartii subsp. stewartii: lessons learned from a xylem-dwelling pathogen of sweet corn. | pantoea stewartii subsp. stewartii is a gram-negative enteric bacterium that primarily infects sweet corn. studies of this bacterium have provided useful insight into how xylem-dwelling bacteria establish themselves and incite disease in their hosts. pantoea stewartii subsp. stewartii is a remarkable bacterial system for laboratory studies because of its relative ease of propagation and genetic manipulation, and the fact that it appears to employ a minimal number of pathogenicity mechanisms. in ... | 2011 | 21726365 |
| mouse model of hypersensitivity pneumonitis after inhalation exposure to different microbial antigens associated with organic dusts. | the aim of this study was to reproduce in laboratory conditions hypersensitivity pneumonitis (hp) pathogenesis in a new animal model predictive of the human response, and to select the microbial antigen associated with organic dust that exerts the strongest pathogenic effect on the respiratory organ. to achieve this goal, mice of the strain c57bl/6j prone to fibrosis were exposed for 1 hour daily up to 28 days to the inhalation of aerosols of each of the 5 microbial components of organic dusts w ... | 2011 | 21736281 |
| a multiphasic approach for the identification of endophytic bacterial in strawberry fruit and their potential for plant growth promotion. | this study used a multiphasic approach, characterized by the simultaneous use of culture-dependent and culture-independent methods, to investigate endophytic bacterial communities in strawberry (fragaria ananassa) fruit. a total of 92 bacterial endophytes were isolated and initially grouped by their repetitive extragenic palindromic (rep)-pcr banding pattern and biochemical features. phylogenetic analysis of the 16s rrna gene sequences of 45 representatives showed that the isolates belonged to t ... | 2011 | 21837472 |
| bacterial community composition of biological degreasing systems and health risk assessment for workers. | biological degreasing system is a new technology based on the degradation capabilities of microorganisms to remove oil, grease, or lubricants from metal parts. no data is available about the potential biological health hazards in such system. thus, a health risk assessment linked to the bacterial populations present in this new degreasing technology is, therefore, necessary for workers. we performed both cultural and molecular approaches in several biological degreasing systems for various indus ... | 2011 | 21698403 |
| antibiosis activity of pantoea agglomerans biocontrol strain e325 against erwinia amylovora on apple flower stigmas. | pantoea agglomerans e325, the active ingredient in a commercial product for fire blight control, was previously shown in vitro to produce a unique alkaline- and phosphate-sensitive antibiotic specific to erwinia amylovora. antibiosis was evaluated as a mode of antagonism on flower stigmas using two antibiosis-deficient mutants. on king's medium b, mutants e325ad1 and e325ad2 have stable smooth-butyrous or hypermucoid colony morphologies, respectively, and the parental strain e325 exhibits phenot ... | 2011 | 21679036 |
| exposure to bioaerosols in the selected agricultural facilities of the ukraine and poland - a review. | the aim of this work was to review the studies on bioaerosols which were carried out in the years 1972-2009 in following branches of agricultural industry in the ukraine and poland: animal farms for cows and pigs, animal feed facilities, production of biofuel from rape, herb farms and herb processing facilities. in all facilities were determined: concentration of dust and microorganisms in the air and species composition of microflora. moreover, in polish animal farms, herb farms and herb proces ... | 2011 | 21736265 |
| antibiotic resistance in bacteria isolated from vegetables with regards to the marketing stage (farm vs. supermarket). | the aim of this study was to elucidate whether and to what extent fresh produce from germany plays a role as a carrier and reservoir of antibiotic resistant bacteria. for this purpose, 1001 vegetables (fruit, root, bulbous vegetables, salads and cereals) were collected from 13 farms and 11 supermarkets in germany and examined bacteriologically. phenotypic resistance of enterobacter cloacae (n=172); enterobacter gergoviae (n=92); pantoea agglomerans (n=96); pseudomonas aeruginosa (n=295); pseudom ... | 2011 | 21700353 |
| pantoea rodasii sp. nov., pantoea rwandensis sp. nov. and pantoea wallisii sp. nov., three novel pantoea species isolated from eucalyptus. | several gram-negative, facultatively anaerobic bacterial isolates were obtained from eucalyptus seedlings showing symptoms of bacterial blight and dieback in colombia, rwanda and south africa. partial 16s rrna gene sequencing, together with partial gyrb sequencing, placed the isolates in the genus pantoea and indicated they constituted three novel species. multilocus sequence analysis (mlsa) based on partial sequences of gyrb, rpob, infb and atpd revealed p. dispersa, p. eucrina and p. cypripedi ... | 2011 | 21841003 |
| impact of derepressed ampc {beta}-lactamase act-9 on the clinical efficacy of ertapenem. | an in vivo development of pantoea agglomerans mutants (isolates pa2 to pa4) with reduced ertapenem susceptibility from that of isolate pa1 was associated with an inadequate clinical response to ertapenem therapy. all four isolates harbored the bla(act-9) ampc +¦-lactamase gene. however, a loss-of-function mutation in the ampd gene in pa2 to pa4, but not pa1, led to derepressed act-9. the reduced ertapenem susceptibility caused by derepressed act-9 was confirmed with an ampd knockout mutant of pa ... | 2011 | 21690276 |
| Sharing of quorum-sensing signals and role of interspecies communities in a bacterial plant disease. | Pathogenic bacteria interact not only with the host organism but most probably also with the resident microbial flora. In the knot disease of the olive tree (Olea europaea), the causative agent is the bacterium Pseudomonas savastanoi pv. savastanoi (Psv). Two bacterial species, namely Pantoea agglomerans and Erwinia toletana, which are not pathogenic and are olive plant epiphytes and endophytes, have been found very often to be associated with the olive knot. We identified the chemical signals t ... | 2011 | 21677694 |
| vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital. | an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ... | 2011 | 21917010 |
| Comparative genomics of the type VI secretion systems of Pantoea and Erwinia species reveals the presence of putative effector islands that may be translocated by the VgrG and Hcp proteins. | ABSTRACT: | 2011 | 22115407 |
| Complete Genome Sequence of the Rice Pathogen Pantoea ananatis Strain PA13. | Pantoea ananatis is the causative agent of sheath and grain rot in rice. Here, we present the complete genome sequence of P. ananatis strain PA13, originally isolated from a diseased rice grain. | 2012 | 22207741 |
| Proposal to reclassify Brenneria quercina (Hildebrand & Schroth 1967) Hauben et al. 1999 into a novel genus, Lonsdalea gen. nov., as Lonsdalea quercina comb. nov., descriptions of Lonsdalea quercina subsp. quercina comb. nov., Lonsdalea quercina subsp. iberica subsp. nov. and Lonsdalea quercina subsp. britannica subsp. nov., emendation of the description of the genus Brenneria, reclassification of Dickeya dieffenbachiae as Dickeya dadantii subsp. dieffenbachiae comb. nov., and emendation of the description of Dickeya dadantii. | Bacterial isolates from oak in Spain and Britain, showing symptoms of bark canker and Acute Oak Decline (AOD) respectively, were examined by a polyphasic approach. Both 16S rRNA gene sequencing and multilocus sequence analysis (MLSA), based on partial sequences of gyrB, rpoB, infB and atpD, revealed that the isolates were separated into two genetic groups according to their origin. Their closest phylogenetic relative was B. quercina, the causal agent of drippy nut disease of oak, which clustered ... | 2011 | 21890733 |
| high isoprenoid flux escherichia coli as a host for carotenoids production. | a noncarotenogenic microbe e. coli was engineered for high production of carotenoids. to increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (dxs, idi and ispdispf) in the isoprenoid pathway were replaced with a strong bacteriophage t5 promoter (p(t5)) by using the λ-red recombinase system in combination with the flp/frt site-specific recombination system for marker excision and p1 transduction for gene trait stacking. the resulting high isoprenoid flux e ... | 2012 | 22144352 |
| Pantoea clinical isolates cannot be accurately assigned to species based on metabolic profiling. | | 2011 | 22093950 |
| screening of freeze-dried protective agents for the formulation of biocontrol strains, bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40. | the effects of different freeze-drying protective agents on the viabilities of biocontrol strains bacillus cereus ar156, burkholderia vietnamiensis b418 and pantoea agglomerans 2re40 were investigated. | 2012 | 22017666 |
| Emergence of blaCTX-M-15, qnrB1 and aac(6')-Ib-cr resistance genes in Pantoea agglomerans and Enterobacter cloacae from Nigeria (sub-Saharan Africa). | | 2012 | 21921107 |
| The genetic and structural basis of two distinct terminal side branch residues in stewartan and amylovoran exopolysaccharides and their potential role in host adaptation. | Stewartan and amylovoran exopolysaccharide (EPS) produced by the plant pathogenic bacteria Pantoea stewartii and Erwinia amylovora are virulence factors in the cause of Stewart's vascular wilt and fire blight. The biosynthesis of amylovoran and stewartan is encoded by a set of homologous operons that have been partially characterized, although some annotations are solely on the basis of sequence homology. The major distinguishing features of these two EPS forms are the presence of a terminal pyr ... | 2012 | 22111898 |
| culturable bacterial communities on leaf sheaths and panicles of rice plants in japan. | culturable bacterial communities on rice plants were investigated from 2001 to 2003. in total, 1,394 bacterial isolates were obtained from the uppermost leaf sheaths at 1 month before heading time and from leaf sheaths and panicles at heading time. the average culturable bacterial population on the leaf sheaths was larger at heading time than at 1 month previously. furthermore, the population was significantly larger on panicles than on leaf sheaths, suggesting that the bacterial population is i ... | 2011 | 22081407 |
| Functional characterization of lipopolysaccharide derived from symbiotic bacteria in rice as a macrophage-activating substance. | Lipopolysaccharide derived from a symbiotic bacterium in wheat (Pantoea agglomerans, LPSp) has shown multiple positive effects, such as prophylactic, antiallergic and antitumour effects, without serious side-effects. LPSp has differential biological activities in comparison to other LPS, such as those from Escherichia coli (LPSe). The only difference between LPSp and LPSe is in the O-antigen polysaccharide structure (O-PS). This led us to the hypothesis that the O-PS structure would seem to part ... | 2011 | 21873161 |
| Gamma-radiation induced modifications in substrate specificity of glucose dehydrogenase and carbon source utilization pattern of phosphate-solubilizing Pantoea strains. | Glucose, maltose, and mannose as sole carbon sources, induced synthesis of glucose dehydrogenase (GDH) in three strains of Pantoea with specific activities from 0.14 to 0.6 U/mg proteins. Utilization of lactose indicated that the enzyme belongs to GDH type B isozyme. Of mutant clones, developed through radiation mutagenesis, P2-M2 utilized ribose with GDH specific activity of 0.57 U/mg protein, P4-M3 grown on glucose gave 1.5 U/mg protein and P4-M5 had high activities, when grown on galactose, m ... | 2011 | 21866443 |
| hessian fly-associated bacteria: transmission, essentiality, and composition. | plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. gall midges are one of the largest groups of insects that manipulate host plants extensively. hessian fly (hf, mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. to examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with hf was performed for the first time. diverse bacteria were found in different de ... | 2011 | 21858016 |
| characterization of phragmites cummunis rhizosphere bacterial communities and metabolic products during the two stage sequential treatment of post methanated distillery effluent by bacteria and wetland plants. | this study deals with the characterization of rhizosphere bacterial communities and metabolic products produced during the two stage sequential treatment of post methanated distillery effluent by bacteria and constructed wetland plants. results showed that bacterial treatment followed by wetland plants (phragmites cummunis) resulted 94.5% and 96.0% reduction in bod and cod values, respectively. the pcr-rflp analysis showed the presence of stenotrophomonas, enterobacter, pantoea, acinetobacter an ... | 2012 | 22047662 |
| spm43.1 contributes to acid-resistance of non-symplasmata-forming cells in pantoea agglomerans ys19. | pantoea agglomerans ys19 is a rice endophytic bacterium characterized to form multicellular biofilm-like structures called symplasmata. phenotypic distinctions between symplasmata-forming cells and planktonic cells are crucial for understanding ys19's survival strategies. in this study, a 43.1 kda protein spm43.1 was identified to show significant resistance to the aggregation effect caused by denaturing acidic conditions. maldi-tof analysis data indicated that it is a maltose-binding protein ho ... | 2011 | 22134847 |
| lipopolysaccharide ip-pa1 from pantoea agglomerans prevents suppression of macrophage function in stress-induced diseases. | chronic psychological stress impairs health and induces various diseases by causing an imbalance in the immune, neuropsychiatric and endocrine systems. the primary reason for the development of stress-induced disease is suppression of macrophage function, which plays a pivotal role in innate immunity. in fact, surgical stress has been shown to exacerbate opportunistic infections by significantly suppressing macrophage function. conversely, administration of macrophage activating substances befor ... | 2011 | 21873156 |
| antibiotic resistance and extended-spectrum β-lactamases in isolated bacteria from seawater of algiers beaches (algeria). | the aim of the study was to evaluate bacterial antibiotic resistance in seawater from four beaches in algiers. the most significant resistance rates were observed for amoxicillin and ticarcillin, whereas they were relatively low for ceftazidime, cefotaxime and imipenem. according to sampling sites, the highest resistance rates were recorded for 2 sites subjected to chemical and microbiological inputs (amoxicillin, 43% and 52%; ticarcillin, 19.6% and 47.7%), and for 2 sites relatively preserved f ... | 2011 | 22095134 |
| The complete genome sequence of Pantoea ananatis AJ13355, an organism with great biotechnological potential. | Pantoea ananatis AJ13355 is a newly identified member of the Enterobacteriaceae family with promising biotechnological applications. This bacterium is able to grow at an acidic pH and is resistant to saturating concentrations of L-glutamic acid, making this organism a suitable host for the production of L-glutamate. In the current study, the complete genomic sequence of P. ananatis AJ13355 was determined. The genome was found to consist of a single circular chromosome consisting of 4,555,536 bp ... | 2011 | 22159605 |
| probing the impact of ligand binding on the acyl-homoserine lactone-hindered transcription factor esar of pantoea stewartii subsp. stewartii. | the quorum-sensing regulator esar from pantoea stewartii subsp. stewartii is a luxr homologue that is inactivated by acyl-homoserine lactone (ahl). in the corn pathogen p. stewartii, production of exopolysaccharide (eps) is repressed by esar at low cell densities. however, at high cell densities when high concentrations of its cognate ahl signal are present, esar is inactivated and derepression of eps production occurs. thus, esar responds to ahl in a manner opposite to that of most luxr family ... | 2011 | 21949066 |
| dinitrogen fixation of microbe-plant associations as affected by nitrate and ammonium supply. | abstract the dinitrogen fixation activity of azospirillum sp., and pantoea agglomerans strains was determined by (15)n(2) incorporation after incubation with (15)n(2) labeled air or/and by acetylene reduction. these bacterial strains were able to fix n(2) both in pure culture and in association with wheat plants in hydroponics. nitrogenase activity of azospirillum sp., in pure culture was more rapidly inhibited by the addition of nh(4) (+) than no(3) (-). the n(2) fixation of p. agglomerans de ... | 1997 | 22087483 |
| Bacterial skin flora variation and in vitro inhibitory activity against Saprolegnia parasitica in brown and rainbow trout. | Variations in the number and diversity of bacteria from the skin of brown trout Salmo trutta L. and rainbow trout Oncorhynchus mykiss Walbaum were surveyed from different rivers and fish farms in northern Spain. In addition to determining bacterial populations in skin samples of healthy fish, bacterial populations were determined from skin lesions (of brown trout only) infected with Saprolegnia parasitica, the causal agent of saprolegniosis. Mean bacterial counts from skin lesions of brown trout ... | 2011 | 22013752 |
| Cell aggregation is negatively regulated by N-acylhomoserine lactone-mediated quorum sensing in Pantoea ananatis SK-1. | Pantoea ananatis SK-1 produces N-acylhomoserine lactones (AHLs) and regulates expression of some virulence factors through AHL-mediated quorum sensing. In this study, we discovered that the strain SK-02I, which has a disrupted AHL-synthetic gene, showed constitutive cell aggregation. SK-1 has the ability to aggregate, and cell aggregation inhibitory factors are expressed under control of AHL-mediated quorum sensing. One of the transposon mutants, SK-33M, constitutively aggregates without defecti ... | 2011 | 21900041 |
| cow teat skin, a potential source of diverse microbial populations for cheese production. | the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ... | 2012 | 22081572 |
| multiple origins of endosymbiosis within the enterobacteriaceae (gamma-proteobacteria): convergence of complex phylogenetic approaches. | abstract: background: the bacterial family enterobacteriaceae gave rise to a variety of symbiotic forms, from the loosely associated commensals, often designated as secondary (s) symbionts, to obligate mutualists, called primary (p) symbionts. determination of the evolutionary processes behind this phenomenon has long been hampered by the unreliability of phylogenetic reconstructions within this group of bacteria. the main reasons have been the absence of sufficient data, the highly derived nat ... | 2011 | 22201529 |
| efficient synthesis of hydroxystyrenes via biocatalytic decarboxylation/deacetylation of substituted cinnamic acids by newly isolated pantoea agglomerans strains. | background: decarboxylation of substituted cinnamic acids is a predominantly followed pathway for obtaining hydroxystyrenes-one of the most extensively explored bioactive compounds in the food and flavor industry (e.g. fema gras approved 4-vinylguaiacol). for this, mild and green strategies providing good yields with high product selectivity are needed. results: two newly isolated bacterial strains, i.e. pantoea agglomerans kjlpb4 and p. agglomerans kjpb2, are reported for mild and effective dec ... | 2011 | 21919002 |
| Isolation of Bacteria with Antifungal Activity against the Phytopathogenic Fungi Stenocarpella maydis and Stenocarpella macrospora. | Stenocarpella maydis and Stenocarpella macrospora are the causal agents of ear rot in corn, which is one of the most destructive diseases in this crop worldwide. These fungi are important mycotoxin producers that cause different pathologies in farmed animals and represent an important risk for humans. In this work, 160 strains were isolated from soil of corn crops of which 10 showed antifungal activity against these phytopathogens, which, were identified as: Bacillus subtilis, Pseudomonas spp., ... | 2011 | 22016606 |
| Endospore production allows using spray-drying as a possible formulation system of the biocontrol agent Bacillus subtilis CPA-8. | The role of endospore production by Bacillus subtilis CPA-8 on survival during spray-drying was investigated by comparison with a non-spore-forming biocontrol agent Pantoea agglomerans CPA-2. Endospore formation promoted heat resistance in CPA-8 depending on growth time (72 h cultures were more resistant than 24 h ones). The survival of CPA-8 and CPA-2 after spray-drying was determined after being grown in optimised media for 24 and 72 h. Spray-dried 72 h CPA-8 had the best survival (32%), while ... | 2011 | 22187082 |
| pantoea stewartii subsp. stewartii produces an endoglucanase that is required for full virulence in sweet corn. | pantoea stewartii subsp. stewartii (pnss), a xylem-dwelling bacterium, is the causal agent of stewart's wilt and blight of sweet corn. the goal of this study was to characterize the only gene in the pnss genome predicted to encode an endoglucanase; this gene was designated engy. the recombinant engy protein and culture supernatants of pnss possessed both egase and xylanase activities. deletion of engy abolished egase and xylanase activity demonstrating that engy appears to be the major egase/x ... | 2011 | 22122328 |
| E622, a miniature, virulence-associated mobile element. | Miniature inverted terminal repeat elements (MITEs) are nonautonomous mobile elements that have a significant impact on bacterial evolution. Here we characterize E622, a 611-bp virulence-associated MITE from Pseudomonas syringae, which contains no coding region but has almost perfect 168-bp inverted repeats. Using an antibiotic coupling assay, we show that E622 is transposable and can mobilize an antibiotic resistance gene contained between its borders. Its predicted parent element, designated T ... | 2012 | 22081398 |
| The Type III Effector HsvG of the Gall-Forming Pantoea agglomerans Mediates Expression of the Host Gene HSVGT. | The type III effector HsvG of the gall-forming Pantoea agglomerans pv. gypsophilae (Pag) is a DNA-binding protein that is imported to the host nucleus and involved in host specificity. The DNA-binding region of HsvG was delineated to 266 amino acids located within a secondary structure region near the N-terminus of the protein, but did not display any homology to canonical DNA-binding motifs. A binding site selection procedure was used to isolate a target gene of HsvG, named HSVGT, in Gypsophila ... | 2011 | 21995766 |
| Identification of the Midgut Microbiota of An. stephensi and An. maculipennis for Their Application as a Paratransgenic Tool against Malaria. | The midgut microbiota associated with Anopheles stephensi and Anopheles maculipennis (Diptera: Culicidae) was investigated for development of a paratransgenesis-based approach to control malaria transmission in Eastern Mediterranean Region (EMR). Here, we present the results of a polymerase chain reaction (PCR) and biochemical-based approaches to identify the female adult and larvae mosquitoe microbiota of these two major malaria vectors, originated from South Eastern and North of Iran. Plating ... | 2011 | 22163022 |
| Effects of wilting and molasses addition on fermentation and bacterial community in guinea grass silage. | Aims: Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. Methods and Results: Direct-cut and wilted guinea grass silage was prepared with and without molasses. A high acetic acid level was observed during the fermentation of direct-cut silage, and long storage inc ... | 2011 | 22150390 |
| Characterization and screening of plant probiotic traits of bacteria isolated from rice seeds cultivated in Argentina. | Many seeds carry endophytes, which ensure good chances of seedling colonization. In this work, we have studied the seed-borne bacterial flora of rice varieties cultivated in the northeast of Argentina. Surface-sterilized husked seeds of the rice cultivars CT6919, El Paso 144, CAMBA, and IRGA 417 contained an average of 5×10(6) CFU/g of mesophilic and copiotrophic bacteria. Microbiological, physiological, and molecular characterization of a set of 39 fast-growing isolates from the CT6919 seeds re ... | 2011 | 22203552 |
| massive production of butanediol during plant infection by phytopathogenic bacteria of the genera dickeya and pectobacterium. | plant pathogenic bacteria of the genera dickeya and pectobacterium are broad-host-range necrotrophs which cause soft-rot diseases in important crops. a metabolomic analysis, based on (13)c-nmr spectroscopy, was used to characterize the plant-bacteria interaction. metabolic profiles revealed a decline in plant sugars and amino acids during infection and the concomitant appearance of a compound identified as 2,3-butanediol. butanediol is the major metabolite found in macerated tissues of various h ... | 2011 | 22032684 |
| isolation of endophytic bacteria from arboreal species of the amazon and identification by sequencing of the 16s rrna encoding gene. | endophytic bacteria from three arboreal species native to the amazon (carapa guianenses, ceiba pentandra, and swietenia macrophylla), were isolated and identified, through partial sequencing of the 16s rrna encoding gene. from these, 16 isolates were obtained, although, when compared to sequences deposited in genbank, only seven had produced identifiable fragments. bacillus, pantoea and two non-culturable samples were identified. results obtained through sequence analysis revealed low genetic di ... | 2011 | 22215973 |
| nosocomial outbreak of pantoea agglomerans bacteraemia associated with contaminated anticoagulant citrate dextrose solution: new name, old bug? | we describe an outbreak investigation of pantoea agglomerans bacteraemia associated with anticoagulant citrate-dextrose 46% (acd) solution prepared in-house. a healthy man presented with septic shock during plasmapheresis for granulocyte donation. the solution used for priming and blood samples were sent for culture. identification of the isolate to species level was performed by gyrb sequencing. typing was performed by pulsed-field gel electrophoresis (pfge). in total, eight cases were identifi ... | 2012 | 22245117 |