| the canine hookworm genome: analysis and classification of ancylostoma caninum survey sequences. | hookworms infect nearly a billion people. the ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. as a step towards a full-scale a. caninum genome project, we generated 104,000 genome survey sequences (gsss) and determined the genome size of the canine hookworm. gsss assembled into 57.6 mb of unique sequence from a genome that we estimate b ... | 2008 | 18082904 |
| application of in vitro anthelmintic sensitivity assays to canine parasitology: detecting resistance to pyrantel in ancylostoma caninum. | resistance of the canine hookworm ancylostoma caninum to anthelmintic therapy with pyrantel is an emerging problem in canine veterinary practice. detecting anthelmintic resistance in parasites of pets is problematic because traditional resistance-monitoring techniques used with livestock parasites, such as the faecal egg count reduction test, are often impractical for use in small animals. we used two field-collected isolates of a. caninum in an abbreviated critical trial to test their pyrantel ... | 2008 | 18242867 |
| a novel small heat shock protein 12.6 (hsp12.6) from brugia malayi functions as a human il-10 receptor binding protein. | phage display cdna expression library of the third stage larvae (l3) of brugia malayi was screened for identifying target(s) that bound to the human interleukin-10 receptor (huil10r). this iterative screening identified an insert that showed significant homology to caenorhabditis elegans hsp12.6. the gene was designated b. malayi hsp12.6 (bmhsp12.6) and has orthologues in several gastrointestinal nematode genome (ancylostoma caninum, ascaris lumbricoides and ascaris suum) but the gene or gene pr ... | 2008 | 18395809 |
| a faecal analysis of helminth infections in wild and captive wolves, canis lupus l., in poland. | one hundred and three samples of faeces of reared grey wolves from four locations (stobnica park and zoological gardens in bydgoszcz, wrocław and cracow) and twenty-six samples of faeces from two free-roaming packs of grey wolf (canis lupus l.) in piła (forest divisions: borne sulinowo, czarnobór, jastrowo) and zielona góra (forest divisions: torzym, krosno odrzańskie) were collected between 2005 and 2007. helminth eggs were detected in 78.6% of faecal samples of reared grey wolves and in 88.4% ... | 2010 | 20236557 |
| pcr-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by ancylostoma ceylanicum in temple communities in bangkok. | a survey of gastrointestinal parasites of dogs and humans from temple communities in bangkok revealed that 58% of dogs and 3.4% of humans, among those sampled, were infected with hookworms utilising faecal flotation techniques and microscopy. a previously established polymerase chain reaction (pcr)-rflp approach was utilised to determine the species of hookworms infecting dogs found positive for hookworm eggs. single infections with ancylostoma ceylanicum and ancylostoma caninum were recorded in ... | 2008 | 18556131 |
| plant vermicides of haitian vodou show in vitro activity against larval hookworm. | haitian vodou priests (houngans) and priestesses (mambos) use plant remedies to treat many illnesses, including intestinal parasite infections. the present study screened 12 plants used in vodou treatments for intestinal parasites to detect in vitro activity against infective-stage larvae of the hookworm ancylostoma caninum. water-soluble extracts of 4 of the 12 plants inhibited serum-stimulated feeding by larval a. caninum in a dose-dependent manner. all 4 plant extracts inhibited feeding induc ... | 2008 | 18576795 |
| strategies for the storage of ancylostoma caninum third-stage larvae. | although cryopreservation protocols for storage of hookworm larvae have been described, the circumstances under which the technique is necessary to ensure larval survival are not well defined. the motility of infective-stage larvae (as judged by observation) and their ability to migrate through canine skin in vitro were measured over a 7-mo period in worms held at room temperature and worms that had been cryopreserved at the start of the experiment. cryopreserved worms showed motility and migrat ... | 2008 | 18605779 |
| gastrointestinal and ectoparasites from urban stray dogs in fortaleza (brazil): high infection risk for humans? | dogs are important definite or reservoir hosts for zoonotic parasites. however, only few studies on the prevalence of intestinal parasites in urban areas in brazil are available. we performed a comprehensive study on parasites of stray dogs in a brazilian metropolitan area. we included 46 stray dogs caught in the urban areas of fortaleza (northeast brazil). after euthanization, dogs were autopsied. ectoparasites were collected, and the intestinal content of dogs were examined for the presence of ... | 2010 | 20532563 |
| mitochondrial dna variation of the dog hookworm ancylostoma caninum in brazilian populations. | the mitochondrial cytochrome oxidase i gene was partially sequenced for 164 ancylostoma caninum individuals, originating from five different localities in brazil, with the aim of describing the genetic diversity and genetic structure of brazilian hookworm populations. allelic and nucleotide diversity were moderate (overall h=0.88 and pi=0.016) and were similar among cities. there was moderate genetic differentiation among the populations sampled (approximately phi(st)=0.12) and a weak but nonsig ... | 2008 | 17997042 |
| cytosolic glutathione s-transferases of oesophagostomum dentatum. | oesophagostomum dentatum stages were investigated for glutathione s-transferase (gst) expression at the protein and mrna levels. gst activity was detected in all stages (infectious and parasitic stages including third- and fourth-stage larvae of different ages as well as males and females) and could be dose-dependently inhibited with sulfobromophthalein (sbp). addition of sbp to in vitro larval cultures reversibly inhibited development from third- to fourth-stage larvae. two glutathione-affinity ... | 2008 | 18771610 |
| investigations into the prevention of neonatal ancylostoma caninum infections in puppies by application of imidacloprid 10% plus moxidectin 2.5% topical solution to the pregnant dog. | the aim of the investigation was to examine whether a single topical administration of a combination of imidacloprid and moxidectin to pregnant dogs could prevent neonatal infections with reactivated ancylostoma caninum larvae. three pregnant beagles, infected with a. caninum, were treated topically with the combination on day 56 of pregnancy. three further dogs served as untreated controls. treatment appeared to prevent neonatal infections in the puppies completely. neither intestinal stages no ... | 2009 | 18793274 |
| the prevalence and abundance of helminth parasites in stray dogs from the city of queretaro in central mexico. | the prevalence of helminth species in stray dogs, from the capital city of the state of queretaro, was evaluated. a total of 378 dogs were captured and examined for the presence of helminths from january to december 2008. the results showed that 275 (72.8%) of examined dogs were infected with one or more helminth species. single infections were observed in 139 (50.5%) of infected dogs and 136 (49.5%) harboured mixed infections. out of the 378 dogs examined, 208 (55.2%) presented nematodes and 18 ... | 2010 | 20849669 |
| principal intestinal parasites of dogs in tirana, albania. | from 2004 to 2009, the digestive tracts of 111 dogs from suburban areas around tirana, albania, were examined for intestinal helminths. in addition, rectal faecal samples of all dogs were examined for protozoan infections and 48 faecal samples from dogs >6 months of age were processed with the baermann technique to test for the excretion of lungworm larvae. the heart and pulmonary arteries of 30 dogs >6 months of age also were examined for nematode parasites. the intestinal parasite fauna of the ... | 2010 | 20878182 |
| variations in response to filariform larvae of ancylostoma caninum in the skin of man. | | 1945 | 21013414 |
| intestinal helminths induce haematological changes in dogs from jabalpur, india. | the effect of canine intestinal helminths on the haematological profile of 200 dogs, of both sexes and variable age, visiting university veterinary clinics for routine examination was investigated. the dogs were assigned to parasitized (n = 39) and non-parasitized (n = 161) groups of animals. coprological examination revealed a 19.5% prevalence of different species of the helminths. of these animals, 10.25% had mixed infections with ancylostoma caninum, toxascaris spp. and dipylidium caninum. th ... | 2010 | 21110909 |
| acetylcholine receptor subunit genes from ancylostoma caninum: altered transcription patterns associated with pyrantel resistance. | the molecular mechanism of resistance to nicotinic agonist anthelmintics such as pyrantel and levamisole in nematodes of medical and veterinary significance is poorly understood. the identification of pyrantel-resistant isolates of the canine hookworm, ancylostoma caninum, provides an opportunity to explore, at a molecular level, the mechanism of cholinergic resistance in a species that is a model for the human hookworms. here we describe the cloning of three a. caninum genes orthologous to comp ... | 2009 | 18823982 |
| epidemiology of intestinal helminth parasites in stray dogs from markets in south-eastern nigeria. | a survey of gastrointestinal helminth parasites of stray dogs (canis familiaris) was conducted at obollo-afor and ekwulobia markets, in enugu and anambra states, south-eastern nigeria, respectively, to determine the patterns of infection among dogs in different parts of south-eastern nigeria. faecal samples collected, using long forceps, from every dog encountered in the markets between june 2007 and december 2008 were analysed by the kato-katz technique. out of 413 dogs examined in both markets ... | 2010 | 21144127 |
| characterisation of hookworm heat shock factor binding protein (hsb-1) during heat shock and larval activation. | when hookworm infective l3s infect their mammalian host, they undergo a temperature shift from that of the ambient environment to that of their endothermic host. additionally, l3s living in the environment can be exposed to temperature extremes associated with weather fluctuations. the heat shock response (hsr) is a conserved response to heat shock and other stress that involves the expression of protective heat shock proteins (hsps). the hsr is controlled by heat shock factor-1 (hsf-1), a conse ... | 2010 | 21172351 |
| potential risk of zoonotic infections in recreational areas visited by sus scrofa and vulpes vulpes. case study--wolin island, poland. | the relation between intestinal parasite prevalence in wild boars and red foxes and the sanitary condition of the soil in recreational estates were determined. the analysis was made based on 36 samples of boar faeces and 22 samples of fox faeces, collected in their habitat as well as 60 samples of soil from two recreational areas. two methods were used for faecal samples--flotation and direct faecal smear; and flotation in nano3 for soil samples examination. zoonotic nematode eggs were recovered ... | 2010 | 21174955 |
| recent research developments in the direct inhibition of coagulation proteinases--inhibitors of the initiation phase. | physiologic clotting is a defensive action. the new cell-based model of hemostasis proposes three steps--initiation, amplification and propagation--occurring on specific cell surfaces to generate a thrombus in a tightly regulated manner. the initiation phase relies on key players including tissue factor (tf), factor viia (fviia), platelets, ca2+, phospholipids, and factor x/xa (fx/fxa). exposure of tf on sub-endothelial and other blood cells triggers a coagulation response, which may have to be ... | 2008 | 18855645 |
| molecular cloning and dna binding characterization of daf-16 orthologs from ancylostoma hookworms. | infective hookworm l3s encounter a host-specific signal during infection that re-initiates a suspended developmental pathway, resulting in development to the adult stage. this resumption of development in the host is analogous to recovery of developmentally arrested caenorhabditis elegans dauer larvae in response to favorable environmental signals. dauer recovery in c. elegans dauers and hookworm l3s is mediated by insulin-like signaling (ils). a key output of ils in c. elegans is the forkhead t ... | 2009 | 18930062 |
| efficacy, safety and palatability of a new broad-spectrum anthelmintic formulation in dogs. | the efficacy, safety and palatability of a new flavoured chewable anthelmintic tablet were investigated in dogs. the efficacy, based on worm counts, of a single recommended therapeutic dose (rtd) of 5 mg pyrantel + 20 mg oxantel + 5 mg praziquantel/kg bodyweight was assessed in experimental infections (ei) and natural infections (ni) with trichuris vulpis, echinococcus granulosus and toxocara canis. for t vulpis, the efficacy of the treatment was 99.3 per cent in ei (comparing groups of six trea ... | 2010 | 21257464 |
| larval migration in perl chambers as an in vitro model for percutaneous infection stimulates feeding in the canine hookworm ancylostoma caninum. | ancylostoma caninum third-stage larvae are the non-feeding infective stage of this parasite and are able to infect potential hosts via different infection routes. since percutaneous infection is one of the most important routes and skin penetration is the first step into parasitic life, an existing in vitro model for percutaneous migration was modified and evaluated. the main parameter used to evaluate migration was the migration ratio (migrated larvae as a percentage of total number of larvae r ... | 2011 | 21266069 |
| investigation of the regulation of transcriptional changes in ancylostoma caninum larvae following serum activation, with a focus on the insulin-like signalling pathway. | the exit from dauer in the free-living nematode caenorhabditis elegans is under the control of a single amphidial neuron (asj) of the insulin-like signalling pathway. mutations of this pathway have the ability to suppress entry into the dauer stage. it has been postulated that insulin-like signalling plays a significant role in the response to serum stimulation in vitro of the third-stage larvae (l3s) of the canine hookworm ancylostoma caninum. to test for the possible involvement of the insulin ... | 2009 | 19054616 |
| rnapc2 inhibits colorectal cancer in mice through tissue factor. | recombinant nematode anticoagulant protein c2 (rnapc2) is a specific inhibitor of tissue factor (tf)/factor viia complex with novel antithrombotic activity. tf is highly expressed in human colorectal tumors, and levels are positively correlated with disease progression. | 2009 | 19118048 |
| immune recognition of the surface associated antigen, tc-saa-1, from infective larvae of teladorsagia circumcincta. | a cdna encoding a surface-associated antigen was amplified by reverse transcriptase polymerase chain reaction (pcr) from rna extracted from teladorsagia circumcincta exsheathed third stage larvae (xl3). the protein encoded by this cdna, tc-saa-1, displays 77% identity over 162 amino acid residues to a surface associated antigen from ancylostoma caninum (ac-saa-1). antiserum raised against a bacterially-expressed recombinant form of tc-saa-1 reacted with a native protein in somatic and surface ex ... | 2009 | 19121081 |
| expression and characterization of aspartic protease gene in eggs and larvae stage of ancylostoma caninum. | hookworm infection is still a public health problem in developing countries. aspartic protease plays an important role in parasite invasion and migration in the host. aspartic protease gene from ancylostoma caninum has been reported (biochem biophys res commun 227:294-302, 1996), but the activity of acasp in eggs and larvae stage has not been studied. this paper reported the cloning of acasp, expression in escherichia coli, and characterization in eggs and larval stage. the mrna encoding acasp w ... | 2009 | 19169710 |
| rna and protein synthesis is required for ancylostoma caninum larval activation. | the developmentally arrested infective larva of hookworms encounters a host-specific signal during invasion that initiates the resumption of suspended developmental pathways. the resumption of development during infection is analogous to recovery from the facultative arrested dauer stage in the free-living nematode caenorhabditis elegans. infective larvae of the canine hookworm ancylostoma caninum resume feeding and secrete molecules important for infection when exposed to a host mimicking signa ... | 2011 | 21354706 |
| an epidemiological study of intestinal parasites of dogs from yucatan, mexico, and their risk to public health. | abstract the prevalence of intestinal parasites in dogs and factors associated were studied in a rural community of yucatan (southern mexico), with special attention to those gastrointestinal parasites potentially transmitted to man. one hundred thirty dogs from 91 households were studied. fecal samples were processed by the centrifugation-flotation and the mcmaster techniques. to determine factors associated with zoonotic parasites in dogs, univariate analysis was performed, using sex, age, and ... | 2011 | 21395413 |
| slo-1-channels of parasitic nematodes reconstitute locomotor behaviour and emodepside sensitivity in caenorhabditis elegans slo-1 loss of function mutants. | the calcium-gated potassium channel slo-1 in caenorhabditis elegans was recently identified as key component for action of emodepside, a new anthelmintic drug with broad spectrum activity. in this study we identified orthologues of slo-1 in ancylostoma caninum, cooperia oncophora, and haemonchus contortus, all important parasitic nematodes in veterinary medicine. furthermore, functional analyses of these slo-1 orthologues were performed using heterologous expression in c. elegans. we expressed a ... | 2011 | 21490955 |
| gastrointestinal parasites and their prevalence in the arabian red fox (vulpes vulpes arabica) from the kingdom of saudi arabia. | the gastrointestinal parasites and prevalence of infestation in the arabian red fox vulpes vulpes arabica thomas, were investigated at the king khalid wildlife research centre (kkwrc) in thumamah, riyadh province, saudi arabia. faecal samples were collected from 58 wild caught foxes while under anaesthesia and examined for gastrointestinal parasites stages. male and female foxes were infected with three major groups of parasites; cestodes, nematodes, protozoa as well as an acanthocephalan. faeca ... | 2011 | 21524855 |
| the mitochondrial genomes of ancylostoma caninum and bunostomum phlebotomum--two hookworms of animal health and zoonotic importance. | hookworms are blood-feeding nematodes that parasitize the small intestines of many mammals, including humans and cattle. these nematodes are of major socioeconomic importance and cause disease, mainly as a consequence of anaemia (particularly in children or young animals), resulting in impaired development and sometimes deaths. studying genetic variability within and among hookworm populations is central to addressing epidemiological and ecological questions, thus assisting in the control of hoo ... | 2009 | 19210793 |
| comparison of passive fecal flotation run by veterinary students to zinc-sulfate centrifugation flotation run in a diagnostic parasitology laboratory. | the sensitivity of fecal examination methods can be influenced by both technician error and methodology. in this analysis, we compared the results of 335 passive fecal flotation examinations performed on the feces of stray dogs by 3rd-yr veterinary students at the university of pennsylvania, school of veterinary medicine, to the results obtained through zinc-sulfate centrifugation performed by the diagnostic parasitology laboratory on the same fecal samples. the students' passive flotation resul ... | 2009 | 19284803 |
| efficacy of emodepside plus toltrazuril (procox(®) oral suspension for dogs) against toxocara canis, uncinaria stenocephala and ancylostoma caninum in dogs. | the efficacy of emodepside plus toltrazuril (pro-and between 4 and 655 worms for hookworms. the cox(®) oral suspension for dogs) against different studies demonstrated 100 % efficacy of emodepside/ species of gastrointestinal nematodes (toxocara toltrazuril suspension against mature adult, =94.7% canis, ancylostoma caninum, uncinaria steno-efficacy against immature adult and 99.3 % efficacy cephala) was evaluated in nine randomised, against the l4 larval stage of t. canis. the efficacy blinded a ... | 2011 | 21739370 |
| real-time pcr assays for monitoring benzimidazole resistance-associated mutations in ancylostoma caninum. | frequent and broad application of anthelmintic drugs for treatment of intestinal parasite infection has led to drug resistance that often renders whole populations of livestock unresponsive to treatment. therefore, it is important to detect mutations associated with drug resistance before it becomes clinically manifest. to monitor developing drug resistance against benzimidazoles (bz), we developed real-time pcr assays and applied them to analyse the beta-tubulin isotype-1 gene of the hookworm a ... | 2009 | 19545525 |
| dose-response assay templates for in vitro assessment of resistance to benzimidazole and nicotinic acetylcholine receptor agonist drugs in human hookworms. | with the implementation of mass drug administration programs for the control of human hookworms, there is a need to monitor for the emergence of drug resistance. we have therefore examined in vitro assays for monitoring sensitivity to benzimidazoles (egg hatch assay) and nicotinic acetylcholine receptor agonist drugs (motility and morphology assays), with a view to developing tools for monitoring drug sensitivity in the field. we have performed assays with necator americanus and ancylostoma ceyl ... | 2009 | 19556583 |
| identification of an anticoagulant peptide that inhibits both fxia and fviia/tissue factor from the blood-feeding nematode ancylostoma caninum. | factor viia-tissue factor complex (fviia/tf) and factor xia (fxia) play important roles in the initiation and amplification of coagulation, respectively. they may be good targets for the development of novel anticoagulants to treat and prevent thromboembolic disease. in this study, we cloned, expressed and identified a novel anticoagulant peptide, acanap10, from the blood-feeding nematode ancylostoma caninum. acanap10 showed potent anticoagulant activity and doubled the activated partial thrombo ... | 2010 | 20059979 |
| a history of hookworm vaccine development. | the human hookworms necator americanus and ancylostoma duodenale remain among the most common infections of humans in areas of rural poverty in the developing regions of the world, with an estimated 1 billion people infected with one or more of these parasites. herein, we review the nearly 100 years of research, development, animal testing, and fieldwork that have led to our current progress in recombinant hookworm vaccines. we begin with the identification of hookworm at the start of the 20th c ... | 2011 | 22064562 |
| molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione s-transferases from the human hookworm necator americanus. | hookworm glutathione s-transferases (gsts) are critical for parasite blood feeding and survival and represent potential targets for vaccination. three cdnas, each encoding a full-length gst protein from the human hookworm necator americanus (and designated na-gst-1, na-gst-2, and na-gst-3, respectively) were isolated from cdna based on their sequence similarity to ac-gst-1, a gst from the dog hookworm ancylostoma caninum. the open reading frames of the three n. americanus gsts each contain 206 a ... | 2010 | 20145100 |
| male-enriched transcription of genes encoding asps and kunitz-type protease inhibitors in ancylostoma species. | various transcripts coding for proteins considered to be central to parasite-host interactions were identified previously as male-enriched in the hookworm ancylostoma braziliense. among these genes were an asp-5-like homologue and a kunitz-type protease inhibitor. the present study extends this previous work to investigate similar molecules in other hookworms (ancylostomatidae). specifically, partial cdna sequences encoding three different asp molecules and two different kunitz-type protease inh ... | 2009 | 19646525 |
| Pharmacokinetics of spinosad and milbemycin oxime administered in combination and separately per os to dogs. | Holmstrom, S. D., Totten, M. L., Newhall, K. B., Qiao, M., Riggs, K. L. Pharmacokinetics of spinosad and milbemycin oxime administered in combination and separately per os to dogs. J. vet. Pharmacol. Therap. doi: 10.1111/j.1365-2885.2011.01333.x. Pharmacokinetic (PK) studies were conducted to determine the potential PK interactions when spinosad and milbemycin oxime (MBO) are administered simultaneously. Investigations used commercial MBO tablets (C-MBO; Interceptor(®) Flavor Tabs, active ingred ... | 2011 | 21895692 |
| prevalence of giardia intestinalis and other zoonotic intestinal parasites in private household dogs of the hachinohe area in aomori prefecture, japan in 1997, 2002 and 2007. | an epidemiological study on canine intestinal parasites was undertaken to evaluate changes in the prevalence among private household dogs from the hachinohe region of aomori prefecture, japan, in 1997, 2002 and 2007, using the formalin-ethyl acetate sedimentation technique. the risk of zoonotic transmission from household dogs to humans was also discussed. all intestinal parasites detected in the present study (giardia intestinalis, isospora spp., toxocara canis, ancylostoma caninum, trichuris v ... | 2009 | 19934595 |
| seroepidemiological survey of helminthic parasites of stray dogs in sari city, northern iran. | the objective of this study was to determine the prevalence rate of helminthic parasites in stray dogs' population especially zoonotic infections and to identify potential risk factors in the different areas of sari city in caspian area, north of iran. during the period from april to september 2007, 50 stray dogs were collected from urban areas of sari city. recovered parasites were fixed in alcohol and stained by carmine then observed by microscope. the taxonomic study was carried out by measur ... | 2011 | 21916265 |
| gender-enriched transcription of activation associated secreted proteins in ostertagia ostertagi. | activation associated secreted proteins (asp) are members of a nematode-specific protein family belonging to the scp/tpx-1/ag5/pr-1/sc7 family. three different types of molecules have been identified in this family: two-domain asps and single-domain asps showing homology to either the c-terminal or n-terminal domain of the two-domain asp. the function of these proteins is still unclear, but a role in transition to parasitism and a role as allergen are often suggested. here we report that the abo ... | 2008 | 17961575 |
| prevalence of selected zoonotic and vector-borne agents in dogs and cats in costa rica. | to estimate the prevalence of enteric parasites and selected vector-borne agents of dogs and cats in san isidro de el general, costa rica, fecal and serum samples were collected from animals voluntarily undergoing sterilization. each fecal sample was examined for parasites by microscopic examination after fecal flotation and for giardia and cryptosporidium using an immunofluorescence assay (ifa). giardia and cryptosporidium ifa positive samples were genotyped after pcr amplification of specific ... | 2011 | 21846585 |
| identification of hookworm daf-16/foxo response elements and direct gene targets. | the infective stage of the parasitic nematode hookworm is developmentally arrested in the environment and needs to infect a specific host to complete its life cycle. the canine hookworm (ancylostoma caninum) is an excellent model for investigating human hookworm infections. the transcription factor of a. caninum, ac-daf-16, which has a characteristic fork head or "winged helix" dna binding domain (dbd), has been implicated in the resumption of hookworm development in the host. however, the preci ... | 2010 | 20808816 |
| prevalence and risk factors of giardia duodenalis in dogs from romania. | the protozoan giardia duodenalis is a mammalian-infecting parasite that produces diarrhoea and malabsorption in its hosts. a survey to investigate canine infections with g. duodenalis in romania was undertaken between june 2008 and december 2009. the objectives of the study were to (i) estimate the prevalence of infection in different dog populations (kennels, shelters, shepherd, household) using microscopy and a commercially available enzyme-linked immunosorbent assay (elisa) test kit; (ii) to ... | 2011 | 21899952 |
| environmental monitoring and analysis of faecal contamination in an urban setting in the city of bari (apulia region, italy): health and hygiene implications. | few studies have been conducted in italy to quantify the potential risk associated with dynamics and distribution of pathogens in urban settings. the aim of this study was to acquire data on the environmental faecal contamination in urban ecosystems, by assessing the presence of pathogens in public areas in the city of bari (apulia region, italy). to determine the degree of environmental contamination, samples of dog faeces and bird guano were collected from different areas in the city of bari ( ... | 2010 | 21139871 |
| angiogenesis and parasitic helminth-associated neovascularization. | successful metazoan parasitism, among many other factors, requires a supply of nutrients and the removal of waste products. there is a prerequisite for a parasite-defined vasculature. the angiogenic mechanism(s) involved presumably depend on the characteristics of the tissue- and vascular system-dwelling, parasitic helminths. simplistically, 2 possibilities or a combination of both have been considered in this review. the multifactorial induction of parasitic helminth-associated neovascularizati ... | 2011 | 21232174 |
| ac-ap-12, a novel factor xa anticoagulant peptide from the esophageal glands of adult ancylostoma caninum. | immunoscreening an ancylostoma caninum cdna library with canine hookworm-infected dog serum resulted in the isolation of a 461 bp cdna encoding ac-ap-12, a new 9.1 kda anticoagulant peptide (100 amino acids) with 43-69% amino acid homology to other nematode anticoagulant peptides (naps) from ancylostoma hookworms. messenger rna transcription and expression of ac-ap-12 was unique to the adult stage of a. caninum. the yeast expressed recombinant ac-ap-12 demonstrated potent anticoagulant activity ... | 2011 | 21251931 |
| intestinal and lung parasites in owned dogs and cats from central italy. | prevalence and risk factors of intestinal and lung parasites were investigated in 239 owned dogs and 81 owned cats from central italy. in 36 dogs and 20 cats found infected by nematodes, pre and post-treatment faecal egg count (fec) was also evaluated. about 31% of dogs and about 35% of cats resulted positive for at least one intestinal or lung parasitic species. helminthic, intestinal and zoonotic infections resulted prevalent in examined animals. examined dogs resulted infected by toxocara can ... | 2012 | 23265188 |
| beta carbonic anhydrases: novel targets for pesticides and anti-parasitic agents in agriculture and livestock husbandry. | the genomes of many insect and parasite species contain beta carbonic anhydrase (β-ca) protein coding sequences. the lack of β-ca proteins in mammals makes them interesting target proteins for inhibition in treatment of some infectious diseases and pests. many insects and parasites represent important pests for agriculture and cause enormous economic damage worldwide. meanwhile, pollution of the environment by old pesticides, emergence of strains resistant to them, and their off-target effects a ... | 2014 | 25174433 |
| dogs, cats, parasites, and humans in brazil: opening the black box. | dogs and cats in brazil serve as primary hosts for a considerable number of parasites, which may affect their health and wellbeing. these may include endoparasites (e.g., protozoa, cestodes, trematodes, and nematodes) and ectoparasites (i.e., fleas, lice, mites, and ticks). while some dog and cat parasites are highly host-specific (e.g., aelurostrongylus abstrusus and felicola subrostratus for cats, and angiostrongylus vasorum and trichodectes canis for dogs), others may easily switch to other h ... | 2014 | 24423244 |
| rangelia vitalii in a free-ranging maned wolf (chrysocyon brachyurus) and co-infections. | an adult free-ranged female maned wolf was rescued from a periurban area subject to anthropogenic disturbances in the minas gerais, brazil. the animal presented poor body condition and anemia. the clinical condition rapidly deteriorated culminating in dead and a necropsy was performed. the main gross lesions were marked anemia and blood content in the intestines accompanied by many types of parasites. the protozoa rangelia vitalii was identified by histopathological analysis predominantly within ... | 2016 | 27761403 |
| vaccination with recombinant brugia malayi cystatin proteins alters worm migration, homing and final niche selection following a subcutaneous challenge of mongolian gerbils (meriones unguiculatus) with b. malayi infective larvae. | cysteine protease inhibitors of brugia malayi have been ascribed to be involved in parasite development as well as to immunomodulate the host's immune response. in onchocerca volvulus, onchocystatin has been shown to induce partial protection in the mouse diffusion chamber vaccination model. in the present study we investigated the impact of vaccination with recombinant bm-cpi-1 and bm-cpi-2 proteins on protection against a subcutaneous challenge of b. malayi third stage larvae in gerbils. | 2014 | 24450869 |
| serine proteases of parasitic helminths. | serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. a number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development and nutrition, host tissues and cell invasion, anticoagulation, and immune evasion. in this review, we de ... | 2015 | 25748703 |
| data on the parasitological status of golden jackal (canis aureus l., 1758) in hungary. | in hungary, twenty canis aureus individuals were submitted to parasitological examinations in 2010-2012. two coccidia: cystoisospora canis (15%) and toxoplasma-type oocysts (5%), one trematoda: alaria alata (10%), six cestoda: mesocestoides lineatus (20%), echinococcus granulosus (10%), dipylidium caninums (5%), taenia hydatigena (15%), taenia pisiformis (20%), taenia crassiceps (40%), and nine nematoda: angiostrongylus vasorum (10%), crenosoma vulpis (30%), capillaria aerophila (5%), toxocara c ... | 2014 | 24334089 |
| levels of infection of intestinal helminth species in the golden jackal canis aureus from serbia. | during the past decade, golden jackal populations have substantially increased, yet little is known of their potential for transmitting parasites within animal and human hosts. in the present study, between 2005 and 2010, 447 jackals from six localities in serbia were examined for intestinal parasites. two species of trematodes (alaria alata, pseudamphistomum truncatum), three nematodes (toxocara canis, ancylostoma caninum, gongylonema sp.), and seven cestodes (taenia pisiformis, taenia hydatige ... | 2015 | 23941681 |
| epizootic and zoonotic helminths of the bobcat (lynx rufus) in illinois and a comparison of its helminth component communities across the american midwest. | a total of 6257 helminths of 19 taxa were recovered from the digestive tract and lungs of 67 bobcats in illinois. infections caused by alaria mustelae, diphyllobothrium latum, and macracanthorhynchus ingens are reported for the first time in bobcats. from all the taxa recovered, only three species occurred in high prevalence and caused intense infections: taenia rileyi, alaria marcianae, and toxocara cati, with prevalence and mean intensity of 70% and 6; 42% and 193, and 25% and 14 individuals, ... | 2014 | 24521984 |
| prevalence of dog intestinal nematode parasites in south central west virginia, usa. | coprological examination was used to determine prevalence of gastrointestinal helminthes in a sample of 231 dogs (117 females and 114 males) during the summer of 2009 at a veterinary clinic in south central west virginia, usa. clinical signs (e.g., diarrhea, vomiting, weight gain or loss) were noted in addition to a history of anthelmintic usage. a total of 79 dogs (33.6%) were infected with one or more intestinal nematodes. most dogs (58) were parasitized with a single species, 19 were parasiti ... | 2011 | 21277089 |
| efficacy of afoxolaner plus milbemycin oxime chewable tablets against naturally acquired intestinal nematodes in dogs. | the efficacy of oral afoxolaner plus milbemycin oxime combination chewable tablets (nexgard spectra, merial) against naturally acquired intestinal nematode infections in dogs was evaluated in six negative control, blinded studies including a total of 114 dogs. dogs were selected based on a pre-treatment fecal examination indicating patent infections with hookworms (two studies), toxocara or toxascaris ascarids (one study each) or trichuris whipworms (two studies). in each study, dogs were assign ... | 2016 | 26827857 |
| molecular identification of ancylostoma species from dogs and an assessment of zoonotic risk in low-income households, são paulo state, brazil. | hookworm infection stands out for its worldwide distribution and for its veterinary and public health relevance. based on copromicroscopic examinations and polymerase chain reaction (pcr) amplification of the its1-5.8s-its2 region, we assessed, respectively, the prevalence of intestinal parasites and the identification of canine hookworm species in faeces recovered from 278 dogs living in households of an inland municipality of são paulo state, brazil. intestinal parasites were found in 67.3% of ... | 2017 | 26752269 |
| re-evaluation of the species of hookworms infecting dogs in central vietnam. | differentiation of canine hookworm species is crucial from both a veterinary and public health standpoint. in vietnam, three hookworm species, namely ancylostoma caninum, ancylostoma braziliense and uncinaria stenocephala are reported to infect dogs. in light of the emerging distribution of a. ceylanicum in asia, this study aims to re-evaluate the status of ancylostoma in dogs in vietnam. | 2015 | 26216353 |
| [cutaneous larva migrans after a trip to the caribean]. | cutaneous larva migrans is a parasitic disease caused by ancylostoma braziliense and ancylostoma caninum larvae, which is transmitted by contact with sand infested with these parasites. dogs and cats are the definitive hosts. this parasitic disease is endemic in the caribbean, africa, australia, and asia. we present the case of a 27-year-old woman, who developed skin lesions compatible with cutaneous larva migrans on her right foot after returning from beach vacations in the mexican caribbean. a ... | 2014 | 25146212 |
| first report of ancylostoma ceylanicum in wild canids. | the parasitic nematode ancylostoma ceylanicum is common in dogs, cats and humans throughout asia, inhabiting the small intestine and possibly leading to iron-deficient anaemia in those infected. it has previously been discovered in domestic dogs in australia and this is the first report of a. ceylanicum in wild canids. wild dogs (dingoes and dingo hybrids) killed in council control operations (n = 26) and wild dog scats (n = 89) were collected from the wet tropics region around cairns, far north ... | 2013 | 24533332 |
| ancylostoma caninum: calibration and comparison of diagnostic accuracy of flotation in tube, mcmaster and flotac in faecal samples of dogs. | we performed a calibration of flotation in tube, mcmaster and flotac to determine the optimal flotation solution (fs) and the influence of faecal preservation for the diagnosis of ancylostoma caninum in dogs, and compared the accuracy of the three copromicroscopic techniques. among nine different fs, sodium chloride and sodium nitrate performed best for detection and quantification of a. caninum eggs. faecal samples, either fresh or preserved in formalin 5%, resulted in higher a. caninum egg cou ... | 2011 | 21295030 |
| intestinal parasites of dogs on the galapagos islands. | dogs on the galapagos islands are a unique population created by isolation from the mainland and regulations prohibiting further importation. the effect of infectious agents of these domestic dogs on the indigenous fauna is largely unknown. the purpose of this study was to determine the prevalence of intestinal parasites in dogs on the galapagos islands. fecal samples were collected from 97 dogs presented during neutering campaigns on santa cruz (n=51), san cristobal (n=17), and isabela (n=29) i ... | 2010 | 20176441 |
| hookworms of dogs and cats as agents of cutaneous larva migrans. | dogs and cats are hosts to hookworms that may cause zoonotic disease, most notably, cutaneous larva migrans. ancylostoma braziliense is most often implicated in dermatological lesions, and ancylostoma caninum has been associated with eosinophilic enteritis and suggested as a possible cause of diffuse unilateral subacute neuroretinitis in humans. other manifestations include eosinophilic pneumonitis, localized myositis, folliculitis, erythema multiforme, or ophthalmological manifestations. ancylo ... | 2010 | 20189454 |
| drug target prediction and prioritization: using orthology to predict essentiality in parasite genomes. | new drug targets are urgently needed for parasites of socio-economic importance. genes that are essential for parasite survival are highly desirable targets, but information on these genes is lacking, as gene knockouts or knockdowns are difficult to perform in many species of parasites. we examined the applicability of large-scale essentiality information from four model eukaryotes, caenorhabditis elegans, drosophila melanogaster, mus musculus and saccharomyces cerevisiae, to discover essential ... | 2010 | 20361874 |
| characterizing ancylostoma caninum transcriptome and exploring nematode parasitic adaptation. | hookworm infection is one of the most important neglected diseases in developing countries, with approximately 1 billion people infected worldwide. to better understand hookworm biology and nematode parasitism, the present study generated a near complete transcriptome of the canine hookworm ancylostoma caninum to a very high coverage using high throughput technology, and compared it to those of the free-living nematode caenorhabditis elegans and the parasite brugia malayi. | 2010 | 20470405 |
| massively parallel sequencing and analysis of the necator americanus transcriptome. | the blood-feeding hookworm necator americanus infects hundreds of millions of people worldwide. in order to elucidate fundamental molecular biological aspects of this hookworm, the transcriptome of the adult stage of necator americanus was explored using next-generation sequencing and bioinformatic analyses. | 2010 | 20485481 |
| activation of nippostrongylus brasiliensis infective larvae is regulated by a pathway distinct from the hookworm ancylostoma caninum. | developmentally arrested infective larvae of strongylid nematodes are activated to resume growth by host-derived cues encountered during invasion of the mammalian host. exposure of nippostrongylus brasiliensis infective larvae to elevated temperature (37°c) is sufficient to activate signalling pathways which result in resumption of feeding and protein secretion. this occurs independently of exposure to serum or glutathione, in contrast to the hookworm ancylostoma caninum, and is not initiated by ... | 2010 | 20654619 |
| protective effects of a bacterially expressed nif-kgf fusion protein against bleomycin-induced acute lung injury in mice. | current evidence suggests that the keratinocyte growth factor (kgf) and the polymorphonuclear leukocyte may play key roles in the development of lung fibrosis. here we describe the construction, expression, purification, and identification of a novel nif (neutrophil inhibitory factor)-kgf mutant fusion protein (nkm). the fusion gene was ligated via a flexible octapeptide hinge and expressed as an insoluble protein in escherichia coli bl21 (de3). the fusion protein retained the activities of kgf ... | 2010 | 20705596 |
| an elisa for sensitive and specific detection of circulating antigen of angiostrongylus vasorum in serum samples of naturally and experimentally infected dogs. | canine angiostrongylosis is an emerging cardiopulmonary disease in europe which can be fatal if left untreated. we developed a sandwich-elisa based on a monoclonal antibody (mab av 56/1/2) and on polyclonal rabbit antibodies directed against angiostrongylus vasorum adult excretory/secretory - antigen for the detection of circulating serum antigen of a. vasorum. the sensitivity of the test was 95.7% (78.1-99.9, 95% ci) as determined with sera of 23 dogs naturally infected with a. vasorum. the spe ... | 2011 | 21333451 |
| a coproantigen diagnostic test for strongyloides infection. | accurate diagnosis of infection with the parasite strongyloides stercoralis is hampered by the low concentration of larvae in stool, rendering parasitological diagnosis insensitive. even if the more sensitive agar plate culture method is used repeated stool sampling is necessary to achieve satisfactory sensitivity. in this manuscript we describe the development of a coproantigen elisa for diagnosis of infection. polyclonal rabbit antiserum was raised against strongyloides ratti excretory/secreto ... | 2011 | 21347447 |
| prevalence of canine gastrointestinal helminths in urban lusaka and rural katete districts of zambia. | faecal samples were collected from january 2010 through september 2010 to determine the prevalence of gastrointestinal (gi) helminths infestation in dogs in urban lusaka and rural katete districts of zambia. a total of 452 faecal samples (n=160 katete, n=292 lusaka) were examined by faecal flotation for the presence of helminth eggs and 82.5% of dogs were positive for gi helminths in katete compared to 76% for lusaka. positive results with the presence of at least one parasite corresponded to 72 ... | 2011 | 21612833 |
| rnai effector diversity in nematodes. | while rna interference (rnai) has been deployed to facilitate gene function studies in diverse helminths, parasitic nematodes appear variably susceptible. to test if this is due to inter-species differences in rnai effector complements, we performed a primary sequence similarity survey for orthologs of 77 caenorhabditis elegans rnai pathway proteins in 13 nematode species for which genomic or transcriptomic datasets were available, with all outputs subjected to domain-structure verification. our ... | 2011 | 21666793 |
| generation of bioactive recombinant ancylostoma caninum anticoagulant peptide c2. | thrombus formation is a crucial factor in the precipitation of unstable angina or myocardial infarction. recently, several anticoagulant serine protease inhibitors have been identified from adult ancylostoma caninum hookworms. one of them, a. caninum anticoagulant peptide c2 (acapc2), can inhibit the activity of factor viia/tissue factor complex to exert its antithrombotic effect. however, it is difficult to adopt traditional expression and purification systems to yield high-purity recombinant a ... | 2011 | 21907287 |
| hookworm scp/taps protein structure-a key to understanding host-parasite interactions and developing new interventions. | scp/taps proteins are a diverse family of molecules in eukaryotes, including parasites. despite their abundant occurrence in parasite secretomes, very little is known about their functions in parasitic nematodes, including blood-feeding hookworms. current information indicates that scp/taps proteins (called ancylostoma-secreted proteins, asps) of the canine hookworm, ancylostoma caninum, represent at least three distinct groups of proteins. this information, combined with comparative modelling, ... | 2011 | 22120067 |
| transgenic c. elegans dauer larvae expressing hookworm phospho null daf-16/foxo exit dauer. | parasitic hookworms and the free-living model nematode caenorhabtidis elegans share a developmental arrested stage, called the dauer stage in c. elegans and the infective third-stage larva (l3) in hookworms. one of the key transcription factors that regulate entrance to and exit from developmental arrest is the forkhead transcription factor daf-16/foxo. during the dauer stage, daf-16 is activated and localized in the nucleus. daf-16 is negatively regulated by phosphorylation by the upstream kina ... | 2011 | 22016799 |
| characterization of cathepsin b proteinase (accp-2) in eggs and larvae stages of hookworm ancylostoma caninum. | cathepsin b proteinase constitutes a large multigenes family in parasitic and non-parasitic nematodes. the localization of cathepsin b proteinases (accp-1 and accp-2) in adult worm of ancylostoma caninum has been characterized (harrop et al., 1995), but the localization and function in eggs and larval stages remained undiscovered. here we described the expressing of cathepsin b proteinase (accp-2) in escherichia coli, and immuno-localization of cathepsin b proteinase in eggs and larvae stages of ... | 2011 | 21925175 |
| cloning and characterization of a novel gene encoding 16 kda protein (ac16) from angiostrongylus cantonensis. | angiostrongylus cantonensis is the most common infectious cause of eosinophilic meningitis or meningoencephalitis. a novel gene (ac16) was isolated from a cdna library of a. cantonensis fourth-stage larvae. the putative 16-kda protein has 149 amino acids and is homologous to an immunodominant hypodermal antigen (iha16) from ancylostoma caninum (identities = 57%). in this paper, we cloned the gene and purified the recombinant ac16 (rac16) protein. real-time quantitative pcr revealed that ac16 was ... | 2011 | 22146998 |
| Utility of capsule endoscopy for evaluating anthelmintic efficacy in fully conscious dogs. | The current accepted standard for evaluating the efficacy of gastrointestinal anthelmintic drugs is necropsy of infected animals followed by a comparison of worm counts between treated and non-treated groups. In this study capsule endoscopy, a minimally invasive method of imaging the small intestine of humans, is evaluated as a possible alternative to necropsy for the purposes of worm quantification in dogs. Eighteen Beagle dogs were included in this study. These dogs were part of a separate tri ... | 2011 | 22075243 |
| insights into the membrane interactions of the saposin-like proteins na-slp-1 and ac-slp-1 from human and dog hookworm. | saposin-like proteins (saplips) from soil-transmitted helminths play pivotal roles in host-pathogen interactions and have a high potential as targets for vaccination against parasitic diseases. we have identified two non-orthologous saplips from human and dog hookworm, na-slp-1 and ac-slp-1, and solved their three-dimensional crystal structures. both proteins share the property of membrane binding as monitored by liposome co-pelleting assays and monolayer adsorption. neither saplip displayed any ... | 2011 | 21991310 |
| Confirmation of the efficacy of a combination tablet of spinosad and milbemycin oxime against naturally acquired infections of canine intestinal nematode parasites. | Four separate controlled and blinded studies were conducted to confirm the dose of spinosad and milbemycin oxime (MO) administered orally in combination to dogs for the treatment and control of naturally acquired infections of adult whipworm (Trichuris vulpis), hookworm (Ancylostoma caninum) and ascarids (Toxocara canis, Toxascaris leonina). Dogs were allocated randomly based on pre-treatment quantitative nematode egg counts of each species of interest to one of two treatment groups of 10 or 11 ... | 2011 | 22115944 |
| dose confirmation and non-interference evaluations of the oral efficacy of a combination of milbemycin oxime and spinosad against the dose limiting parasites, adult cat flea (ctenocephalides felis) and hookworm (ancylostoma caninum), in dogs. | two separate controlled and blinded studies were conducted to confirm the dose and non-interference of spinosad and milbemycin oxime (mo) administered orally in combination or alone to dogs for the treatment and control of experimentally induced flea infestations (ctenocephalides felis) and adult hookworm infections (ancylostoma caninum). for each study, dogs were allocated randomly based on pre-treatment adult flea and hookworm egg counts to one of four treatment groups of 10 animals each. in e ... | 2011 | 22226761 |
| [parasitic infections of coyote, canis latrans (carnivora: canidae) in a costa rican national park and a surrounding agricultural area]. | as human populations expand into wild habitats with their pets and livestock, the potential spread of disease to wildlife or vice versa increases. because, wild and domestic canids may pose as reservoirs or disseminators of infectious diseases (including parasites), coyotes (canis latrans) may also serve as indicators of ecological health. in costa rica, little information exists on coyote parasites, making research necessary to identify potential zoonotic interactions. for this reason, a survey ... | 2012 | 23894947 |
| neutrophil inhibitory factor selectively inhibits the endothelium-driven transmigration of eosinophils in vitro and airway eosinophilia in ova-induced allergic lung inflammation. | leukocyte adhesion molecules are involved in cell recruitment in an allergic airway response and therefore provide a target for pharmaceutical intervention. neutrophil inhibitory factor (nif), derived from canine hookworm (ancylostoma caninum), binds selectively and competes with the a-domain of cd11b for binding to icam-1. the effect of recombinant nif was investigated. intranasal administration of rnif reduced pulmonary eosinophilic infiltration, goblet cell hyperplasia, and th(2) cytokine pro ... | 2012 | 23304174 |
| efficacy of oral afoxolaner plus milbemycin oxime chewables against induced gastrointestinal nematode infections in dogs. | the efficacy of oral afoxolaner plus milbemycin oxime combination chewables against induced gastrointestinal nematode infections in dogs was evaluated in six separate studies. two studies were performed to evaluate the efficacy of the product against toxocara canis, two studies evaluated the efficacy against toxascaris leonina, one study evaluated the efficacy against ancylostoma braziliense, and one study evaluated the efficacy against ancylostoma caninum. in the a. caninum study, the efficacy ... | 2016 | 27369586 |
| evaluation of parasitological and immunological aspects of acute infection by ancylostoma caninum and ancylostoma braziliense in mixed-breed dogs. | this study compared the course of infection by ancylostoma caninum and ancylostoma braziliense in mixed-breed dogs infected with l3 larvae. dogs infected with a. caninum eliminated more eggs than did those infected with a. braziliense. a total of 38 % of a. caninum and 44 % of a. braziliense larvae were recovered as adult worms. there were no marked clinical abnormalities in dogs with either infection. a. caninum was associated with anemia and an increased number of circulating neutrophils, wher ... | 2013 | 23494155 |
| rapid detection and identification of human hookworm infections through high resolution melting (hrm) analysis. | hookworm infections are still endemic in low and middle income tropical countries with greater impact on the socioeconomic and public health of the bottom billion of the world's poorest people. in this study, a real-time polymerase chain reaction (pcr) coupled with high resolution melting-curve (hrm) analysis was evaluated for an accurate, rapid and sensitive tool for species identification focusing on the five human hookworm species. | 2012 | 22844538 |
| morphological differentiation of eggs of ancylostoma caninum, ancylostoma tubaeforme, and ancylostoma braziliense from dogs and cats in the united states. | the establishment of cat- and dog-derived laboratory strains of ancylostoma braziliense allowed for a morphological comparison of the eggs of a. braziliense, ancylostoma caninum, and ancylostoma tubaeforme. the length, width, and perimeter were determined for images of 10 eggs each of a. braziliense from the feces of a dog infected with a canine isolate and a cat infected with a feline isolate, a. caninum from dog feces, and a. tubaeforme from cat feces. the specific identity of the eggs was ver ... | 2012 | 22394087 |
| obtaining an isolate of ancylostoma braziliense from dogs without the need for necropsy. | isolation of a specific ancylostoma species typically requires death of the source animal, or holding an animal long enough to collect feces after treatment, for worm recovery and identification. the reason for collecting worms is that the eggs are not easy to distinguish morphologically. in keeping with the 3 rs of laboratory animal research (reduction, refinement, replacement), the objective of this study was to obtain an isolate of ancylostoma braziliense from 1-time field-collected samples o ... | 2012 | 22393913 |
| epidemiological and genetic data supporting the transmission of ancylostoma ceylanicum among human and domestic animals. | currently, information on species-specific hookworm infection is unavailable in malaysia and is restricted worldwide due to limited application of molecular diagnostic tools. given the importance of accurate identification of hookworms, this study was conducted as part of an ongoing molecular epidemiological investigation aimed at providing the first documented data on species-specific hookworm infection, associated risk factors and the role of domestic animals as reservoirs for hookworm infecti ... | 2012 | 22347515 |
| development of new microsatellites for the hookworm ancylostoma caninum and analysis of genetic diversity in brazilian populations. | considering the great efforts towards formulating a vaccine against hookworms, and the concerns about the spread of drug resistance through hookworm populations, it is justified to study the molecular diversity and population genetic structure of these nematodes. this work had the aim to develop microsatellite markers to investigate the genetic structure and the molecular diversity of brazilian populations of ancylostoma caninum. seven microsatellites markers were successfully used to characteri ... | 2017 | 28288926 |
| changes in protein expression after treatment with ancylostoma caninum excretory/secretory products in a mouse model of colitis. | different reports have highlighted the potential use of helminths and their secretions in the treatment of inflammatory bowel disease (ibd) conditions; however, no reports have investigated their effects at a proteome level. herein, we characterise the protein expression changes that occur in lamina propria (lp) and the intestinal epithelial cells (iec) of mice with dextran sulfate sodium (dss)-induced colitis treated with ancylostoma caninum excretory/secretory (es) products using a quantitativ ... | 2017 | 28191818 |
| identification of acap5 as a novel factor xa inhibitor with both direct and allosteric inhibition. | ancylostoma caninum anticoagulant peptide 5 (acap5) is a potent inhibitor for coagulation factor xa (fxa). previous studies show that acap5 binds to fxa at the active site, and/or the exosite. the active site-binding contributes to direct blocking of fxa catalytic activity, but the effect of exosite-binding and the underlying mechanism remain unknown. to investigate whether and how the exosite-binding affects fxa function, we prepared several acap5 mutants with modifications to the active site-b ... | 2017 | 28007598 |
| helminth infections in domestic dogs from russia. | dogs are the hosts for a wide helminth spectrum including tapeworms, flatworms, and nematodes. these parasites affect the dog health and cause morbidity and mortality, especially in young and old animals. some species, as toxocara canis, ancylostoma caninum, dipylidium caninum, and echinococcus spp. are well-known zoonotic parasites worldwide, resulting in high public health risks. poor data about canine helminth species and prevalence are available in russia, mainly due to the absence of offici ... | 2016 | 27956777 |
| gastrointestinal helminths of coyotes (canis latrans) from southeast nebraska and shenandoah area of iowa. | this survey was carried out on the carcasses of 29 coyotes from southeastern nebraska and shenandoah area of iowa to document the helminths present in the intestinal track of these carnivorous animals. | 2016 | 27733798 |
| neotropical zoonotic parasites in bush dogs (speothos venaticus) from upper paraná atlantic forests in misiones, argentina. | wildlife remains an important source of zoonotic diseases for the most vulnerable groups of humans, primarily those living in rural areas or coexisting with forest. the upper paraná atlantic forest of misiones, argentina is facing ongoing environmental and anthropogenic changes, which affect the local biodiversity, including the bush dog (speothos venaticus), a small canid considered near threatened globally and endangered locally. this project aimed to expand the knowledge of zoonotic parasites ... | 2016 | 27603553 |