| bacterial profiles of oral streptococcal and periodontal bacterial species in saliva specimens from japanese subjects. | recent developments in molecular biological techniques have increased understanding of the distribution of oral bacterial species in clinical specimens, though few investigations have been conducted to simultaneously detect oral streptococcal and periodontal species in the same specimens. the purpose of the present study was to investigate the distribution and correlation of 6 oral streptococcal and 6 periodontal species in saliva specimens taken from children and their mothers. | 2009 | 19230860 |
| relationship of neutrophil phagocytosis and oxidative burst with the subgingival microbiota of generalized aggressive periodontitis. | polymorphonuclear neutrophil (pmn) dysfunctions have been associated with severe forms of periodontitis. this study evaluated the correlation between pmn phagocytosis and oxidative burst with the subgingival microbiota of patients with generalized aggressive periodontitis (gagp). | 2009 | 19239639 |
| an orthologue of bacteroides fragilis nanh is the principal sialidase in tannerella forsythia. | sialidase activity is a putative virulence factor of the anaerobic periodontal pathogen tannerella forsythia, but it is uncertain which genes encode this activity. characterization of a putative sialidase, siahi, by others, indicated that this protein alone may not be responsible for all of the sialidase activity. we describe a second sialidase in t. forsythia (tf0035), an orthologue of bacteroides fragilis nanh, and its expression in escherichia coli. sialidase activity of the expressed nanh wa ... | 2009 | 19304852 |
| prediction of a caspase-like fold in tannerella forsythia virulence factor prth. | tannerella forsythia is a bacterial pathogen involved in periodontal disease. a cysteine protease prth has been characterized in this bacterium as a virulence factor. prth has the activity of detaching adherent cells from substratum, and the level of prth is associated with periodontal attachment loss. no reports exist on the structure, active site, and catalytic mechanism of prth. using comparative sequence and structural analyses, we have identified homologs of prth in a number of bacterial an ... | 2009 | 19305154 |
| red bacterial complex is associated with the severity of chronic periodontitis in a thai population. | the distribution of periodontal pathogens differs in various geographic locations and racial/ethnic groups. this study investigated the microbiological features of chronic periodontitis (cp) patients in thailand. | 2009 | 19371397 |
| are putative periodontal pathogens reliable diagnostic markers? | periodontitis is one of the most common chronic inflammatory diseases. a number of putative bacterial pathogens have been associated with the disease and are used as diagnostic markers. in the present study, we compared the prevalence of oral bacterial species in the subgingival biofilm of generalized aggressive periodontitis (gap) (n = 44) and chronic periodontitis (cp) (n = 46) patients with that of a periodontitis-resistant control group (pr) (n = 21). the control group consisted of subjects ... | 2009 | 19386852 |
| the oxyr homologue in tannerella forsythia regulates expression of oxidative stress responses and biofilm formation. | tannerella forsythia is an anaerobic periodontal pathogen that encounters constant oxidative stress in the human oral cavity due to exposure to air and reactive oxidative species from coexisting dental plaque bacteria as well as leukocytes. in this study, we sought to characterize a t. forsythia orf with close similarity to bacterial oxidative stress response sensor protein oxyr. to analyse the role of this oxyr homologue, a gene deletion mutant was constructed and characterized. aerotolerance, ... | 2009 | 19389765 |
| the impact of the stone age diet on gingival conditions in the absence of oral hygiene. | the objective of this study was to assess the oral microbiota and clinical data in subjects without access to traditional oral hygiene methods and who ate a diet available in the stone age. | 2009 | 19405829 |
| the microbiota on different oral surfaces in healthy children. | knowledge of the early oral colonization patterns could provide a better understanding of oral biofilm development and disease initiation that in turn could be the basis for early preventive programmes. | 2009 | 19416446 |
| essential oils in one-stage full-mouth disinfection: double-blind, randomized clinical trial of long-term clinical, microbial and salivary effects. | this randomized clinical trial evaluated the effects of an essential oils-containing mouthrinse for full-mouth disinfection. | 2009 | 19426180 |
| [markers of periodontal diseases and sensitivity to taromentine in patients with aggressive periodontitis]. | the aim of the research was to study sensitivity of specific microorganisms from the periodontal pockets of patients with rapidly progressive periodontal disease to taromentine. 95 patients aged 21 to 35 years (50 women (52,6+/-33,62) and 45 men (47,36+/-3,62)) with rapidly progressive form of periodontal desease were observed. porphiromonas gingivalis was identifide in 83 out of 95 patients (87,36+/-2,06). prevotella intermedia - in 31 patients (32,6+/-2,750); actinobacillus actinomycetemcomita ... | 2009 | 19430039 |
| the use of perioscan as a potential screening test for mothers at risk for delivery of pre-term and low birth-weight infants. | increased levels of oestrogen and progesterone during pregnancy may lead to periodontal disease. the anaerobic gram-negative bacteria classified as the "red complex" (porphyromonas gingivalis, tannerella forsythia and treponema denticola) are frequently associated with periodontal disease. periodontopathogens produce toxins and enzymes that can enter the bloodstream and cross the placenta to harm the foetus. the response of the mother's immune system to infection by these periodontopathogens bri ... | 2009 | 19431959 |
| probiotic effects of orally administered lactobacillus salivarius wb21-containing tablets on periodontopathic bacteria: a double-blinded, placebo-controlled, randomized clinical trial. | this study was designed to evaluate whether the oral administration of lactobacilli could change the bacterial population in supra/subgingival plaque. | 2009 | 19453574 |
| leucine-rich repeats of bacterial surface proteins serve as common pattern recognition motifs of human scavenger receptor gp340. | scavenger receptors are innate immune molecules recognizing and inducing the clearance of non-host as well as modified host molecules. to recognize a wide pattern of invading microbes, many scavenger receptors bind to common pathogen-associated molecular patterns, such as lipopolysaccharides and lipoteichoic acids. similarly, the gp340/dmbt1 protein, a member of the human scavenger receptor cysteine-rich protein family, displays a wide ligand repertoire. the peptide motif vevlxxxxw derived from ... | 2009 | 19465482 |
| systemic immune responses in pregnancy and periodontitis: relationship to pregnancy outcomes in the obstetrics and periodontal therapy (opt) study. | our previous studies reported on the obstetric, periodontal, and microbiologic outcomes of women participating in the obstetrics and periodontal therapy (opt) study. this article describes the systemic antibody responses to selected periodontal bacteria in the same patients. | 2009 | 19485826 |
| major proteins and antigens of treponema denticola. | treponema denticola is a gram-negative, motile, asaccharolytic, anaerobic spirochaete which along with porphyromonas gingivalis and tannerella forsythia has been shown to form a bacterial consortium called the red complex that is strongly associated with the clinical progression of chronic periodontitis. t. denticola was grown in continuous culture in a complex medium with a mean generation time of 15.75 h. samples from two different membrane-enriched preparations and a cytoplasm-enriched prepar ... | 2009 | 19501677 |
| rheumatoid arthritis is linked to oral bacteria: etiological association. | the purpose of this review is to evaluate the association between rheumatoid arthritis (ra) and periodontopathic bacteria. clinical studies of ra and periodontal disease have provided evidence for a significant association between the two disorders. patients with long-standing active ra have a substantially increased frequency of periodontal disease compared with that among healthy subjects. high levels of oral anaerobic bacterial antibodies have been found in the serum and synovial fluid of ra ... | 2009 | 19554393 |
| clinical effectiveness of photodynamic therapy in the treatment of periodontitis. | a randomized-controlled clinical pilot trial was designed to evaluate photodynamic therapy (pdt) for its bactericidal potential and clinical effect in the treatment of periodontitis. | 2009 | 19554711 |
| detection and quantification of five major periodontal pathogens by single copy gene-based real-time pcr. | periodontitis is a common chronic multibacterial infection in the tooth-supporting tissues. it has been shown that periodontitis patients carry higher number of disease-associated bacteria than healthy ones. the aim of this study was to generate a novel, single copy gene-based quantitative real-time pcr (qpcr) assay for five major periodontal pathogens - aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, treponema denticola, and tannerella forsythia. the prim ... | 2009 | 19586997 |
| characterizing traditionally defined periodontal disease in hiv+ adults. | results have varied from previous studies examining the level and extent of periodontal disease (pd) in hiv-1 infected (hiv+) adults. these studies used different methodologies to measure and define pd and examined cohorts with divergent characteristics. inconsistent methodological approaches may have resulted in the underestimation of traditionally-defined pd in hiv+ individuals. | 2009 | 19624697 |
| microbiological profile of untreated subjects with localized aggressive periodontitis. | the microbial profile of localized aggressive periodontitis (lagp) has not yet been determined. therefore, the aim of this study was to evaluate the subgingival microbial composition of lagp. | 2009 | 19637996 |
| microbiological risk for periodontitis of abutment teeth in patients with removable partial dentures. | tannerella forsythia, porphyromonas gingivalis and treponema denticola are identified as the red complex which exists in high proportions in periodontally diseased patients. the aim of this study was to assess the microbiological risk for periodontitis of abutment teeth in subjects wearing removable partial dentures (rpds) by measuring the red complex using bana-zymetm test. thirty-eight subjects (mean age: 62.2 years) with kennedy class ii partially edentulous arch participated in this study. t ... | 2009 | 19650858 |
| full-mouth ultrasonic debridement associated with amoxicillin and metronidazole in the treatment of severe chronic periodontitis. | the purpose of this study was to evaluate the adjunctive clinical, microbiologic, and immunologic effects of the systemic administration of amoxicillin and metronidazole in the full-mouth ultrasonic debridement of patients with severe chronic periodontitis. | 2009 | 19656025 |
| bone microbial contamination influences autogenous grafting in sinus augmentation. | the oral occurrence of putative microbial pathogens in humans has been documented in health and disease. the presence of periodontopathogens in patients with a history of periodontal disease may have a negative impact on bone regeneration. this investigation was conducted to confirm the presence of periodontal pathogens in bone particles harvested intraorally for maxillary sinus augmentation and to assess the clinical and radiographic outcomes 6 to 12 months after bone augmentation. | 2009 | 19656037 |
| strong and persistent microbial and inflammatory stimuli overcome the genetic predisposition to higher matrix metalloproteinase-1 (mmp-1) expression: a mechanistic explanation for the lack of association of mmp1-1607 single-nucleotide polymorphism genotypes with mmp-1 expression in chronic periodontitis lesions. | our objective was to evaluate the association between the mmp1-1607 single-nucleotide polymorphism (snp), periodontopathogens and inflammatory cytokines with matrix metalloproteinase-1 (mmp-1) mrna levels in vitro and in vivo. | 2009 | 19659894 |
| outer membrane proteome and antigens of tannerella forsythia. | tannerella forsythia is a gram-negative, anaerobic, fusiform bacterium implicated as a periodontal pathogen. with use of 2d page, sds page, and lc-maldi-tof/tof ms, 221 proteins of t. forsythia outer membrane preparations were identified, of which 197 were predicted to be localized to the cell envelope. fifty-six proteins were reproducibly mapped by 2d page and included several highly abundant proteins in the mw range 140-250 kda that exhibited c-terminal sequence similarity to the ctd family of ... | 2009 | 19663511 |
| clinical, histological, and microbiological findings in peri-implant disease: a pilot study. | this study is intended to verify the correlation among clinical indices of the peri-implant soft tissues, the histological condition and the presence of 3 pathogens commonly associated with peri-implant diseases (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia). | 2009 | 19667822 |
| quantitative detection of periodontopathic bacteria in atherosclerotic plaques from coronary arteries. | oral pathogens, including periodontopathic bacteria, are thought to be aetiological factors in the development of cardiovascular disease. in this study, the presence of aggregatibacter actinomycetemcomitans, fusobacterium nucleatum-periodonticum-simiae group, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and tannerella forsythia in atheromatous plaques from coronary arteries was determined by real-time pcr. forty-four patients displaying cardiovascular disease were submi ... | 2009 | 19679682 |
| quantification of periodontal pathogens by paper point sampling from the coronal and apical aspect of periodontal lesions by real-time pcr. | the present study compared the recovery of six periodontal pathogens by paper point samples from two different aspects of periodontal lesions by quantitative real-time polymerase chain reaction (pcr). twenty patients with untreated chronic periodontitis were randomized into two groups. before subgingival instrumentation and after 10 weeks samples in group a were taken first with a paper point half length (hp) of the probing depth, then with a paper point full length (fp) at the same site. in gro ... | 2010 | 19693550 |
| t-rflp-based mcra gene analysis of methanogenic archaea in association with oral infections and evidence of a novel methanobrevibacter phylotype. | increasing evidence suggests a role for methanogenic archaea (methanogens) in human health and disease via syntrophic interactions with bacteria. here we assessed the prevalence and distribution of methanogens and possible associations with bacteria in oral biofilms. | 2009 | 19702957 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| distribution of periodontopathic bacterial species in japanese children with developmental disabilities. | recent developments in molecular biological techniques have enabled rapid detection of periodontopathic bacterial species in clinical specimens. accumulated evidence suggests that detection of specific bacterial species enables identification of subjects at high risk for the onset of periodontitis. we investigated the distribution of 10 selected periodontopathic bacterial species in dental plaque specimens obtained from children with disabilities who were attending daycare centers. | 2009 | 19772671 |
| clinical periodontal and microbiologic parameters in patients with acute myocardial infarction. | the aim of this study was to evaluate the impact of clinical periodontal parameters and the presence of periodontal pathogens in patients with acute myocardial infarction (ami). | 2009 | 19792846 |
| salivary interleukin-1beta concentration and the presence of multiple pathogens in periodontitis. | this study aimed to find salivary enzymes and/or cytokines that would reflect periodontitis, alone or in combination with salivary microbial markers. | 2009 | 19799718 |
| differential ability of periodontopathic bacteria to modulate invasion of human gingival epithelial cells by porphyromonas gingivalis. | periodontitis is a polymicrobial infection caused by selected gram-negative bacteria including porphyromonas gingivalis. host cell invasion by p. gingivalis has been proposed as a possible mechanism of pathogenesis in periodontitis. the aim of the present study was to assess the influence of periodontopathogens on p. gingivalis invasion of gingival epithelial cells in polymicrobial infection. p. gingivalis was tested for its ability to invade a human gingival epithelial cell line ca9-22 in co-in ... | 2009 | 19818393 |
| tannerella forsythia detected in infected root canals using nested pcr. | this study assessed the occurrence of tannerella forsythia in patients with acute and chronic primary endodontic infections. | 2009 | 19824556 |
| progression of chronic periodontitis can be predicted by the levels of porphyromonas gingivalis and treponema denticola in subgingival plaque. | chronic periodontitis is an inflammatory disease of the supporting tissues of the teeth associated with bacteria. diagnosis is achieved retrospectively by clinical observation of attachment loss. predicting disease progression would allow for targeted preventive therapy. the aim of this study was to monitor disease progression in patients on a maintenance program and determine the levels of specific bacteria in subgingival plaque samples and then examine the ability of the clinical parameters of ... | 2009 | 19832799 |
| subgingival bacterial colonization profiles correlate with gingival tissue gene expression. | periodontitis is a chronic inflammatory disease caused by the microbiota of the periodontal pocket. we investigated the association between subgingival bacterial profiles and gene expression patterns in gingival tissues of patients with periodontitis. a total of 120 patients undergoing periodontal surgery contributed with a minimum of two interproximal gingival papillae (range 2-4) from a maxillary posterior region. prior to tissue harvesting, subgingival plaque samples were collected from the m ... | 2009 | 19835625 |
| real-time polymerase chain reaction quantification of porphyromonas gingivalis and tannerella forsythia in primary endodontic infections. | porphyromonas gingivalis and tannerella forsythia are anaerobic bacteria commonly involved in root canal infections. although previous investigations have assessed these species by strictly qualitative approaches, accurate determination of their cell levels by a sensitive quantitative technique may contribute with additional information regarding relevance in pain of endodontic origin. | 2009 | 19840640 |
| microbiologic changes in subgingival plaque after removal of fixed orthodontic appliances. | to evaluate changes that occur in the subgingival microbiota after removal of fixed orthodontic appliances using polymerase chain reaction (pcr). | 2009 | 19852608 |
| oral microbiota of children in a school-based dental clinic. | dental caries disproportionately affects disadvantaged subjects. this study hypothesized that there were greater caries extent and higher levels of caries-associated and anaerobic subgingival bacterial species in oral samples of hispanic and immigrant children compared with non-hispanic and us born children. | 2010 | 19879369 |
| clinical status and detection of periodontopathogens and streptococcus mutans in children with high levels of supragingival biofilm. | knowledge about the presence of some important oral pathogens is an important step in better identifying children at risk for periodontal and/or caries diseases in later life. the purpose of this study was to detect the presence of streptococcus mutans (sm), aggregatibacter actinomycetemcomitans (aa), campylobacter rectus (cr), porphyromonas gingivalis (pg), prevotella intermedia (pi), and tannerella forsythia (tf) in gingival biofilm samples from 196 children, and to assess whether any of these ... | 2009 | 19893968 |
| a novel matrix metalloprotease-like enzyme (karilysin) of the periodontal pathogen tannerella forsythia atcc 43037. | proteases of tannerella forsythia, a pathogen associated with periodontal disease, are implicated as virulence factors. here, we characterized a matrix metalloprotease (mmp)-like enzyme of t. forsythia referred to as karilysin. full-length (without a signal peptide) recombinant karilysin (49.9 kda) processed itself into the mature 18-kda enzyme through sequential autoproteolytic cleavage at both n- and c-terminal profragments. the first cleavage at the asn14-tyr15 peptide bond generated the full ... | 2010 | 19919176 |
| do periodontopathogens disappear after full-mouth tooth extraction? | to monitor the intra-oral microbiological changes after full-mouth extraction using quantitative polymerase chain reaction (qpcr). | 2009 | 19930094 |
| changes in the incidence of periodontal pathogens during long-term monitoring and after application of antibacterial drugs. | the incidence of potential periodontal pathogens (aggregatibacter actinomycetemcomitans, formerly actinobacillus actinomycetemcomitans, tannerella forsythia, porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia and capnocytophaga ochracea) was monitored in patients with chronic periodontitis and in healthy control subjects. two types of studies were carried out in which the composition of the bacterial communities in different niches of the same oral cavity ecosystem was invest ... | 2009 | 19937216 |
| transmission of periodontopathic bacteria from natural teeth to implants. | purpose: prevention of peri-implantitis is essential for the success of implant rehabilitation. infection by periodontopathic bacteria is a major cause of peri-implantitis. the aim of the present study was to identify the source of peri-implant colonization by periodontopathic bacteria. materials and methods: twenty-one patients with implants were enrolled in the study. subgingival plaque samples from the adjacent, occluding, and contralateral natural teeth were collected prior to second-stage s ... | 2009 | 20002682 |
| antibacterial and lipopolysaccharide (lps)-neutralising activity of human cationic antimicrobial peptides against periodontopathogens. | in this study, we investigated the antibacterial activity of eight antimicrobial peptides (amps), comprising four human beta-defensins (hbds), three human neutrophil defensins (hnps) and the cathelicidin ll-37, against two representative periodontopathogens, prevotella intermedia and tannerella forsythia. the neutralising effect of these amps on expression of interleukin (il)-1beta, il-8 and intercellular adhesion molecule 1 (icam-1) induced by lipopolysaccharide (lps) from p. intermedia and t. ... | 2010 | 20006468 |
| association of human t lymphotropic virus 1 amplification of periodontitis severity with altered cytokine expression in response to a standard periodontopathogen infection. | periodontal diseases (pds) are infectious diseases in which periodontopathogens trigger chronic inflammatory and immune responses that lead to tissue destruction. recently, viruses have been implicated in the pathogenesis of pds. individuals infected with human t lymphotropic virus 1 (htlv-1) present with abnormal oral health and a marked increased prevalence of periodontal disease. | 2010 | 20038241 |
| evaluating clinical periodontal measures as surrogates for bacterial exposure: the oral infections and vascular disease epidemiology study (invest). | epidemiologic studies of periodontal infection as a risk factor for cardiovascular disease often use clinical periodontal measures as a surrogate for the underlying bacterial exposure of interest. there are currently no methodological studies evaluating which clinical periodontal measures best reflect the levels of subgingival bacterial colonization in population-based settings. we investigated the characteristics of clinical periodontal definitions that were most representative of exposure to b ... | 2010 | 20056008 |
| microbiologic testing and outcomes of full-mouth scaling and root planing with or without amoxicillin/metronidazole in chronic periodontitis. | it has been suggested that use of systemic antibiotics should be limited to patients with specific microbiologic profiles. the main purpose of the present analysis was to study whether microbiologic testing before therapy was of value in predicting which patients would specifically benefit from adjunctive amoxicillin and metronidazole, given in the context of full-mouth scaling and root planing (srp) within 48 hours. | 2010 | 20059413 |
| subgingival plaque removal using a new air-polishing device. | the purpose of this study is to evaluate the safety, patient acceptance, and short-term microbiologic effect of a new air-polishing device in subjects in maintenance care with residual pockets > or =5 mm. | 2010 | 20059420 |
| the bactericidal effect of a genius nd:yag laser. | to evaluate the 'in vitro' bactericidal effect of the nd:yag laser (genius, mølsgaard dental, copenhagen, denmark) on six periodontal pathogens. | 2010 | 20096084 |
| periodontal bacterial load: a proposed new epidemiological method for periodontal disease assessment. | the purpose of this study was to identify a periodontal clinical measure that correlates with red complex bacteria usually associated with periodontal disease. | 2010 | 20098966 |
| [detection of periodental pathogens in atherosclerotic lesions in patients]. | to detect periodontopathic bacterial dna in atherosclerotic vascular lesions in a group of chinese patients by using polymerase chain reaction (pcr) techniques. | 2010 | 20140039 |
| comparison of two different microbiological test kits for detection of periodontal pathogens. | the aim of this study was to compare the outcome of two different microbiological tests for detection of aggregatibacter actinomycetemcomitans (a.a.), porphyromonas gingivalis (p.g.), tannerella forsythia (t.f.) and treponema denticola (t.d.). | 2010 | 20141366 |
| effects of ultrasonic, electric, and manual toothbrushes on subgingival plaque composition in orthodontically banded molars. | orthodontic appliances hinder mechanical plaque control. in this study, we evaluated the effect of self-performed supragingival plaque removal with ultrasonic, electric, and manual toothbrushes on subgingival plaque composition in orthodontically banded molars. | 2010 | 20152680 |
| the relationship between physiologic halitosis and periodontopathic bacteria of the tongue and gingival sulcus. | to determine the influence of oral status on halitosis, the relationship between halitosis and periodontopathic bacteria present in plaque on the tongue and the subgingival sulcus was examined in 62 periodontally healthy adults. halitosis indicators used were the organoleptic score; gas chromatography results [total volatile sulfur compounds (vscs) = h(2)s + ch(3)sh + (ch(3))(2)s]; halimeter values; and the results of three clinical tests, plaque control record (plcr), plaque index (pli), and to ... | 2010 | 20155507 |
| comparison of periodontal pathogens between cats and their owners. | the periodontal pathogens porphyromonas gingivalis and tannerella forsythia are strongly associated with periodontal disease and are highly prevalent in humans with periodontitis. porphyromonas and tannerella spp. have also been isolated from the oral cavity of cats. the oral microflora in animals was compared with those in humans in earlier studies, but no studies are available on the comparison of the oral microflora from pets and their respective owners. the aim of this study was to determine ... | 2010 | 20189734 |
| a novel sialic acid utilization and uptake system in the periodontal pathogen tannerella forsythia. | tannerella forsythia is a key contributor to periodontitis, but little is known of its virulence mechanisms. in this study we have investigated the role of sialic acid in biofilm growth of this periodontal pathogen. our data show that biofilm growth of t. forsythia is stimulated by sialic acid, glycolyl sialic acid, and sialyllactose, all three of which are common sugar moieties on a range of important host glycoproteins. we have also established that growth on sialyllactose is dependent on the ... | 2010 | 20190043 |
| the microbiota associated with implants restored with platform switching: a preliminary report. | it was demonstrated that implants restored according to a platform-switching concept presented less crestal bone loss than implants restored with standard protocols. the aim of this study is to examine differences between the composition of the peri-implant microbiotas associated with implants restored with the platform-switching approach and implants restored with a standard internal connection protocol. | 2010 | 20192867 |
| oral biofilm architecture on natural teeth. | periodontitis and caries are infectious diseases of the oral cavity in which oral biofilms play a causative role. moreover, oral biofilms are widely studied as model systems for bacterial adhesion, biofilm development, and biofilm resistance to antibiotics, due to their widespread presence and accessibility. despite descriptions of initial plaque formation on the tooth surface, studies on mature plaque and plaque structure below the gum are limited to landmark studies from the 1970s, without app ... | 2010 | 20195365 |
| efficacy of amoxicillin and metronidazole combination for the management of generalized aggressive periodontitis. | the aim of this study is to evaluate the effects of metronidazole-amoxicillin combination on clinical and microbiologic parameters in patients with generalized aggressive periodontitis. | 2010 | 20214441 |
| detection of periodontopathogenic bacteria in pregnant women by traditional anaerobic culture method and by a commercial molecular genetic method. | to culture facultative and strict anaerobic bacteria is a well-established method for analyzing subgingival plaque samples. micro-ident and micro-ident plus (hain lifescience gmbh, nehren, germany) tests are two commercially available rapid pcr-based methods for the identification and quantification of putative periodontopathogen bacteria. in this study, we compared these commercial pcr-based hybridization methods with conventional anaerobic culture technique. a total of 36 subgingival plaque sa ... | 2010 | 20219690 |
| short-term effects of systemic antibiotics during periodontal healing. | to investigate the short-term effects of nonsurgical therapy (scaling and root planing, srp) on the subgingival microbiota in chronic (cp) and aggressive (ap) periodontal disease. | 2010 | 20305865 |
| synergistic effects of lipopolysaccharides from periodontopathic bacteria on pro-inflammatory cytokine production in an ex vivo whole blood model. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia have been strongly associated with chronic periodontitis. this disease is characterized by an accumulation of inflammatory cells in periodontal tissue and subgingival sites. the secretion of high levels of inflammatory cytokines by those cells is believed to contribute to periodontal tissue destruction. the aim of this study was to investigate the inflammatory response of whole blood from periodontitis patients following cha ... | 2010 | 20331798 |
| dna from porphyromonas gingivalis and tannerella forsythia induce cytokine production in human monocytic cell lines. | toll-like receptor 9 (tlr9) expression is increased in periodontally diseased tissues compared with healthy sites indicating a possible role of tlr9 and its ligand, bacterial dna (bdna), in periodontal disease pathology. here, we determine the immunostimulatory effects of periodontal bdna in human monocytic cells (thp-1). thp-1 cells were stimulated with dna of two putative periodontal pathogens: porphyromonas gingivalis and tannerella forsythia. the role of tlr9 in periodontal bdna-initiated cy ... | 2010 | 20331800 |
| do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens? | the presence of periodontal pathogens in the oral cavity may impact implant survival. therefore, this study aimed to determine the prevalence of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, prevotella intermedia, tannerella forsythia, treponema denticola, eikenella corrodens and parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures. | 2010 | 20337663 |
| profiling of subgingival plaque biofilm microflora from periodontally healthy subjects and from subjects with periodontitis using quantitative real-time pcr. | qualitative and quantitative changes of the subgingival plaque biofilm microflora in periodontal pockets are thought to be associated with the development and progression of periodontitis. the aims of the present study were to quantify the proportions of nine periodontitis-associated bacterial species and four streptococcus species in subgingival plaque, and to evaluate their relationship with periodontitis quantitatively. | 2010 | 20337892 |
| clinical periodontal and microbiologic parameters in patients with crohn's disease with consideration of the card15 genotype. | crohn's disease (cd) was reported to have oral manifestations. however, data on periodontal parameters and oral microbiology in cd are rare. recent studies showed associations of variants in the caspase recruitment domain (card)15 gene with cd that are involved in the immune response toward bacterial products. our aim is to investigate the periodontal status and prevalence of periodontal pathogens in patients with cd under consideration of the card15 polymorphism. | 2010 | 20373538 |
| proteolytic inactivation of ll-37 by karilysin, a novel virulence mechanism of tannerella forsythia. | tannerella forsythia is a gram-negative bacterium strongly associated with the development and/or progression of periodontal disease. here, we have shown that a newly characterized matrix metalloprotease-like enzyme, referred to as karilysin, efficiently cleaved the antimicrobial peptide ll-37, significantly reducing its bactericidal activity. this may contribute to the resistance of t. forsythia to the antibacterial activity of ll-37, since their vitality was found not to be affected by ll-37 a ... | 2010 | 20375548 |
| evaluation of two local drug delivery systems as adjuncts to mechanotherapy as compared to mechanotherapy alone in management of chronic periodontitis: a clinical, microbiological, and molecular study. | modern concepts of treating inflammatory periodontal disease aim at changes in the subgingival ecosystems within the periodontal pockets to alter the complex microbial community into a microbiota compatible with good periodontal health. systemic antimicrobial therapy, although effective, involves a relatively high dose with repeated intakes over a prolonged period of time to achieve the required inhibitory concentrations in the sulcular fluid. the adjunctive use of local drug delivery may provid ... | 2009 | 20379409 |
| mixture of periodontopathogenic bacteria influences interaction with kb cells. | the purpose of this study was to investigate the adhesion and invasion of periodontopathogenic bacteria in varied mixed infections and the release of interleukins from an epithelial cell line (kb cells). | 2010 | 20380884 |
| [effect of periodontal mechanical treatment on periodontal pathogenic bacteria in gingival crevicular fluid of chronic periodontitis patients]. | to evaluate the subgingival prevalent rates of 6 periodontal pathogenic bacteria in gingival crevicular fluids of cp patients before and after treatment, to analyze the relationship between the prevalent variance and periodontal clinical parameters, and to provide a microbiologic method of evaluating curative effect and estimating the prognosis. | 2010 | 20396365 |
| periodontal disease-associated micro-organisms in peri-menopausal and post-menopausal women using or not using hormone replacement therapy. a two-year follow-up study. | despite conflicting results on the use of hormone replacement therapy (hrt) there is no doubt that many women benefit from it. women using hrt are known to be more health conscious in general with putative positive implications in the mouth. however, we observed recently in our cohort hardly any difference in oral health status between hrt-users and non-users. there are only a few studies about hrt and oral microbiota. we hypothesized that counts of periodontal micro-organisms are lower in healt ... | 2010 | 20429938 |
| periodontitis associated with chediak-higashi syndrome in a young african american male. | chediak-higashi syndrome (chs) is a rare autosomal recessive genetic disease. the primary defect is abnormal granule formation in the cells secondary to a mutation of a lysosomal trafficking regulator protein. chs patients have immune system abnormalities, bleeding abnormalities, and multiple infections including periodontitis. | 2010 | 20465032 |
| mechanical non-surgical treatment of peri-implantitis: a single-blinded randomized longitudinal clinical study. ii. microbiological results. | peri-implantitis is common in patients with dental implants. we performed a single-blinded longitudinal randomized study to assess the effects of mechanical debridement on the peri-implant microbiota in peri-implantitis lesions. | 2010 | 20507380 |
| periodontitis: a polymicrobial disruption of host homeostasis. | periodontitis, or gum disease, affects millions of people each year. although it is associated with a defined microbial composition found on the surface of the tooth and tooth root, the contribution of bacteria to disease progression is poorly understood. commensal bacteria probably induce a protective response that prevents the host from developing disease. however, several bacterial species found in plaque (the 'red-complex' bacteria: porphyromonas gingivalis, tannerella forsythia and treponem ... | 2010 | 20514045 |
| virulence of major periodontal pathogens and lack of humoral immune protection in a rat model of periodontal disease. | oral diseases (2010) doi: 10.1111/j.1601-0825.2010.01678.x objective: this study was designed to test the hypothesis that periodontal pathogens tannerella forsythia and porphyromonas gingivalis are synergistic in terms of virulence potential using a model of mixed-microbial infection in rats. materials and methods: three groups of rats were infected orally with either t. forsythia or p. gingivalis in mono-bacterial infections or as mixed-microbial infections for 12 weeks and a sham-infected grou ... | 2010 | 20534016 |
| periodontopathic bacteria and herpesviruses in chronic periodontitis. | periodontal disease involves complex interactions of microorganisms and host defenses. this work investigated the associations between putative bacterial pathogens, herpesviruses and chronic periodontitis. subgingival samples were collected from 40 periodontally healthy individuals and from 40 patients with chronic periodontitis with probing depths of < or =3 mm or > or =6 mm. multiplex and nested polymerase chain reactions were used to identify bacterial pathogens and herpesviruses. porphyromon ... | 2010 | 20536751 |
| levels of specific immunoglobulin g to the forsythia detaching factor of tannerella forsythia in gingival crevicular fluid are related to the periodontal status. | forsythia detaching factor (fdf) is a putative virulence factor of tannerella forsythia that induces detachment of adherent cells and interleukin-8 production in human fibroblasts. the objective of the present study was to clarify the relationship between anti-fdf igg levels in gingival crevicular fluid and the clinical status in patients with periodontitis and in healthy subjects. | 2010 | 20572920 |
| gingival overgrowth in subjects under immunosuppressive regimens based on cyclosporine, tacrolimus, or sirolimus. | to assess the prevalence and variables associated with gingival overgrowth (go) in renal transplant recipients medicated with cyclosporine (csa), tacrolimus (tcr), or sirolimus (sir). | 2010 | 20618547 |
| collagenolytic activity in sonic extracts of tannerella forsythia. | the purpose of the present study was to characterize the collagenolytic activity in a sonicated extract of tannerella forsythia and to investigate the activation of prommmp-2 and -9 by the t. forsythia extract. | 2010 | 20619781 |
| characterization of the normal bacterial flora in peri-implant sulci of partially and completely edentulous patients. | to characterize the normal bacterial flora and evaluate the presence of periodontopathogenic bacteria around dental implants and to correlate them with the periodontal flora or, in completely edentulous patients, the alveolar gingival flora. | 2010 | 20657863 |
| the ability of the bana test to detect different levels of p. gingivalis, t. denticola and t. forsythia. | the aim of this study was to evaluate the ability of the bana test to detect different levels of porphyromonas gingivalis, treponema denticola and tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (t0), immediately (t1), 45 (t2) and 60 ... | 2010 | 20658043 |
| [association between phenytoin-induced gingival hyperplasia and periodontopathic bacteria in institutionalized patients with severe motor and intellectual disabilities]. | the association between phenytoin-induced gingival hyperplasia (pigh) and five major periodontopathic bacteria was investigated by real-time pcr in 12 subjects (30.6 +/- 7.95 years of age) with severe motor and intellectual disabilities. the subjects had not received antibiotics or periodontal therapy within three months. a healthy gingival site or a site with the lowest gingival hyperplasia score (minimum site), and a site with the maximum score of gingival hyperplasia (maximum site) on each su ... | 2010 | 20662307 |
| relationship between oral status and prevalence of periodontopathic bacteria on the tongues of elderly individuals. | colonization of periodontopathic bacteria is associated with increased risk of systemic diseases. however, few studies have investigated the relationships between oral status factors and health-related quality of life (hr-qol) and the prevalence of such bacteria in elderly individuals. this study investigated the prevalence of porphyromonas gingivalis, prevotella intermedia, treponema denticola and tannerella forsythia in 165 community-dwelling functionally independent 85-year-old japanese indiv ... | 2010 | 20688951 |
| prevalence of periodontal bacteria in saliva of kuwaiti children at different age groups. | aggregatibacter (formerly actinobacillus) actinomycetemcomitans, tannerella forsythensis and porphyromonas gingivalis and to a lesser extent prevotella intermedia and prevotella nigrescens, are gram-negative species that are associated with destructive periodontitis. studies from different parts of the world have shown variable detection rates of periodontal organisms. hardly any data exist on their carriage in children living in the middle east. this study was designed to determine the detectio ... | 2010 | 20701895 |
| virulence mechanisms of tannerella forsythia. | | 2010 | 20712636 |
| subgingival microbial profiles of smokers with periodontitis. | the subgingival microbiome is largely uncultivated, and therefore, cultivation-based and targeted molecular approaches have limited value in examining the effect of smoking on this community. we tested the hypothesis that the subgingival biofilm is compositionally different in current and never-smokers by using an open-ended molecular approach for bacterial identification. subgingival plaque from deep sites of current and never-smokers matched for disease was analyzed by 16s sequencing. smokers ... | 2010 | 20739702 |
| a quantitative proteomic analysis of biofilm adaptation by the periodontal pathogen tannerella forsythia. | tannerella forsythia is a gram-negative anaerobe that is one of the most prominent inhabitants of the sub-gingival plaque biofilm, which is crucial for causing periodontitis. we have used itraq proteomics to identify and quantify alterations in global protein expression of t. forsythia during growth in a biofilm. this is the first proteomic study concentrating on biofilm growth in this key periodontal pathogen, and this study has identified several changes in protein expression. moreover, we int ... | 2010 | 20806225 |
| a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants. | supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues. | 2011 | 20831669 |
| determinants of periodontopathogens in microbiological monitoring of diabetic patients with periodontitis. | to determine and analyze the frequency of periodontopathogens in microbiological monitoring of diabetic patients with periodontitis. | 2010 | 20844819 |
| virulence of major periodontal pathogens and lack of humoral immune protection in a rat model of periodontal disease. | this study was designed to test the hypothesis that periodontal pathogens tannerella forsythia and porphyromonas gingivalis are synergistic in terms of virulence potential using a model of mixed-microbial infection in rats. | 2010 | 20846155 |
| apical root canal microbiota as determined by reverse-capture checkerboard analysis of cryogenically ground root samples from teeth with apical periodontitis. | bacteria located in the apical root canal system potentially participate in the pathogenesis of apical periodontitis. detection and identification of apical bacteria can be compromised because of limitations in conventional sampling and identification procedures. this study identified several bacterial taxa in the apical and middle/coronal segments of primarily infected root canal system by using pulverized root segments and a culture-independent molecular method. | 2010 | 20850664 |
| investigation of subgingival profile of periodontopathic bacteria using polymerase chain reaction. | periodontopathic bacteria such as porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, tannerella forsythia, campylobacter rectus and treponema denticola play an important role in the initiation and progression of periodontitis. the aim of this investigation was to evaluate the relationship between periodontal clinical parameters and the subgingival profile of periodontopathic bacteria. twenty-six periodontitis patients (23-62 years of age; mean age, 40.2±13.2) with no systemic disea ... | 2010 | 20877160 |
| tannerella forsythia infection-induced calvarial bone and soft tissue transcriptional profiles. | tannerella forsythia is associated with subgingival biofilms in adult periodontitis, although the molecular mechanisms contributing to chronic inflammation and loss of periodontal bone remain unclear. we examined changes in the host transcriptional profiles during a t. forsythia infection using a murine calvarial model of inflammation and bone resorption. tannerella forsythia was injected into the subcutaneous soft tissue over calvariae of balb/c mice for 3 days, after which the soft tissues and ... | 2010 | 20883221 |
| potential of the tannerella forsythia s-layer to delay the immune response. | the periodontal pathogen tannerella forsythia possesses a glycosylated s-layer as an outermost cell decoration. while the s-layer provides a selection advantage to the bacterium in the natural habitat, its virulence potential remains to be investigated. in the present study, the immune responses of human macrophages and gingival fibroblasts upon stimulation with wild-type t. forsythia and an s-layer-deficient mutant were investigated. the mrna expression levels of the pro-inflammatory mediators ... | 2010 | 20929722 |
| the use of ozonated water and 0.2% chlorhexidine in the treatment of periodontitis patients: a clinical and microbiologic study. | the development of periodontal disease has been thought to be associated with several restricted members of the oral anaerobic species, such as black-pigmented porphyromonas species and actinobacillus actinomycetemcomitans (aa), in the subgingival environment. apart from bacteria, certain viruses and fungi that are associated with periodontal disease are also present in the subgingival plaque . | 2010 | 20930341 |
| oxygen tension modulates the cytokine response of oral epithelium to periodontal bacteria. | there is an inverse relationship between pocket depth and pocket oxygen tension with deep pockets being associated with anaerobic bacteria. however, little is known about how the host tissues respond to bacteria under differing oxygen tensions within the periodontal pocket. | 2010 | 20955352 |
| real-time polymerase chain reaction of "red complex" (porphyromonas gingivalis, tannerella forsythia, and treponema denticola) in periradicular abscesses. | the red complex bacteria (porphyromonas gingivalis, tannerella forsythia, and treponema denticola) have been implicated, either singly or in combination, in the development of various forms of periodontal diseases. the aim of this study was to investigate the presence of "red complex" in acute periradicular abscesses by real-time polymerase chain reaction (pcr) method. | 2010 | 20955954 |