| repair by human cells of adenovirus-2 damaged by psoralen plus near ultraviolet light treatment. | adenovirus-2 is damaged by treatment with psoralen plus near-ultraviolet (uv) light is shown by reduced ability to infect human fibroblasts. the apparent sensitivity of the virus to this treatment depended upon the strain of cells used. the virus was 3-4 times more sensitive to the treatment when infecting xeroderma pigmentosum (xp complementation groups a or d) fibroblasts than when infecting normal fibroblasts. dna extracted from virus preparations that had undergone such treatment was analyze ... | 1975 | 1214823 |
| caffeine inhibition of the repair of ultraviolet-irradiated adenovirus in human cells. | caffeine is shown to block repair of ultraviolet-irradiated adenovirus 2 when the irradiated virus infects normal human fibroblasts from a xeroderma pigmentosum (xp) variant. such blockage is not observed when the irradiated virus infects xp fibroblasts belonging to xp complementation group a. thus normal and xp variant cells have a caffeine-sensitive repair process. this may be either excision or an excision dependent repair process because fibroblasts belonging to xp complementation group a ar ... | 1975 | 1214824 |
| [development of optimal conditions for isolation of rhinoviruses]. | when 366 dispensary patients with respiratory diseases were examined in moscow during the period december 1969--may 1971, 49 strains of rhinoviruses were isolated out of which 21 strains were identified as type 48. using eagle's medium with a single set of amino acids and vitamins for isolation rhinoviruses were isolated in 4.7% of cases, empdoying eagle's medium with a double set of amino acids and vitamins in 16% of cases. virus isolation experiments were carried out in hela-bristol cell cultu ... | 1975 | 1220246 |
| [characteristics of structural chromosome aberrations induced by type 1 adenovirus in human and chinese hamster cells in vitro]. | adenovirus multiplies in both studied cultures. in the chinese hamster cells infection is of slowed down, cyclic character. in this case a short subcultivation of cells infected with the virus and the study of the damage distribution in groups of chromosomes are possible. no selectivity was found with respect to the damage of certain group chromosomes. | 1975 | 1221572 |
| proceedings: in vitro assessment of virus-induced tumor immunity in the adenovirus/hamster system. | | 1975 | 1228564 |
| [isoenzymes of lactate and malate dehydrogenases in nuclei of rat embryo fibroblasts infected with oncogenic adenovirus type 12]. | the isoenzymatic spectrum of lactatedehydrogenase (ldg; l-lactate: nad-oxireductase; ec 1.1.1.27) and malatedehydrogenase (mdg; 1-malate: nad-oxireductase; ec 1.1.1.37) in nuclei of rat embryo fibroblast cells infected with adenovirus type 12 was studied at 3, 5, 8, 18 and 24 days of cultivation. the nuclei were isolated according to the method of showo et al. modified by zbarsky and georgiev. proteins were fractionated by disc electrophoresis in polyacrylamide gel. in the course of oncovirus-ce ... | 1975 | 1241176 |
| temperature-sensitive mutants of type 12 adenovirus defective in a late function: protein synthesis and evidence for recombination between mutants in complementation group d. | four temperature sensitive mutants of adenovirus type 12, classified into complementation group d, synthesize most or all of the late virus-specific polypeptides at the restrictive temperature, as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. infections by these mutants under restrictive conditions apparently result in some over production of the virion hexon polypeptide compared to wild type infection. genetic recombination between the four mutants has been demonstrated. ... | 1976 | 1245844 |
| intestinal permeability to large particles in normal and protein-deficient adult rats. | intestinal absorption of ferritin and virus particles was assessed in control and protein-deficient rats using light and electron microscopic procedures. rats weighing 100 to 130 grams were divided into two groups and fed either a 0.5% of 18% lactalbumin diet. at monthly intervals following diet initiation, three rats from each group were administered ferritin (mw-650,000) or adenovirus type 5 (mw is greater than 1,000,000) via ligated jejunal loops either 15 or 30 minutes prior to loop excision ... | 1976 | 1245888 |
| genetic analysis of adenovirus type 2 iii. temperature sensitivity of processing viral proteins. | using sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis of [35s]methionine-labeled adenovirus type 2-infected kb cell extracts, a total of 23 virus-induced polypeptides was detected. this technique was applied to the analysis of the temperature-sensitive mutant, ts 1, which has previously been shown to be defective in a late function. by means of pulse-chase experiments, ts 1 was shown to be defective in the processing of the precursor polypeptide (pre vii) to the major core protein ... | 1976 | 1255847 |
| isolation and characterization of an extremely basic protein from adenovirus type 5. | by starch-gel electrophoresis and a staining method that is highly sensitive for argininyl residues, adenovirus type 5 was found to contain two minor basic polypeptides of extreme cathodic mobility in addition to the two known core proteins. the fastest-migrating polypeptide, named mu protein, and the second fastest polypeptide are found in adenovirions and virus-infected kb cells but not in top components or in uninfected cells. the top components and infected cells contain an additional basic ... | 1976 | 1255865 |
| viral transcription in kb cells infected by temperature-sensitive "early" mutants of adenovirus type 5. | rna:dna hybridization was used to study the synthesis of viral rna in two dna-minus, temperature-sensitive mutants of type 5 adenovirus (h5ts125 and h5ts149) belonging to two different, non-overlapping complementation groups. hybridization competition analysis showed that both mutants transcribed all early gene sequences at the restrictive temperature (41 c). in mutant-infected cells at 41 c, the rate of viral transcription was similar to the rate of early rna synthesis in wild-type virus infect ... | 1976 | 1255869 |
| antiviral, immunosuppressive and antitumour effects of ribavirin. | | 1976 | 1256547 |
| the presence of avian adenoviruses and adeno-associated viruses in healthy chickens. | avian adenoviruses were isolated from 56 of 106 fecal and rectal tissue samples taken from apparently healthy young chickens on 4 farms. only one isolation was made from the 37 samples from 3- and 4-week-old chicks, while the isolation frequency was 64-100% in groups 5 weeks old and older. the 56 adenovirus isolates were classified into 6 serotypes. vurses of 3 or 4 types were found on each farm. avian adeno-associated virus cf antigens were found, using chicken embryos coinfected with an adenov ... | 1976 | 1259656 |
| three new virus-specific low molecular rnas in adenovirus type 2 infected kb cells. | | 1976 | 1259770 |
| hybridization maps of early and late messenger rna sequences on the adenovirus type 2 genome. | | 1976 | 1263260 |
| antitumour activity of adenovirus-12 structural proteins against moloney sarcoma tumours in mice. | when purified fibre and hexon proteins of adenovirus 12 were given intramuscularly to 4-week-old balb/c mice (250-300 mug/mouse) 2 h prior to inoculation with mouse sarcoma virus (0.05 ml of 10(4) ffu/ml) at the same site, significant suppression of tumour growth (p less than 0.001), and rapid regression in tumour size (p less than 0.001) were noted. as a rule, the survival rate in treated mice was also significantly higher than in untreated mice. furthermore, the disease process in treated mice ... | 1976 | 1268030 |
| [effects of colchicine, vinblastine and mercury p-hydroxybenzoate on the multiplication of adenovirus 5 in hela cells]. | after 30 min incubation at 37 with 10(-4) m phmb (p-hydroxymercuribenzoate), monolayers of hela cells are not affected; after 3 h exposure to 10(-5) m colchicin or 2.5 x 10(-5) m vinblastine, hela cells are not altered morphologically but the number of cells is decreasing between 1 and 2 days post treatment. when cells are kept in suspension after trypsinization, their ability to adhere to the petri dishes is not altered with 10(-4) m phmb but only 50% of the cells are able to attach after treat ... | 1976 | 1268311 |
| [synthesis of viral antigens in cell cultures infected with adenovirus and a biologically active preparation of its dna]. | | 1976 | 1269376 |
| the lymphoreticular response to a transplanted adenovirus 12-induced tumour of cba mice. | the growth and structure of a virus-induced tumour has been studied in normal and immunised animals. retardation of tumour growth in immunised animals is accompanied by a lymphoreticular infiltrate; macrophages ingest apparently intact tumour cells. | 1976 | 1271134 |
| synthesis of the adenovirus-coded dna binding protein in infected cells. | synthesis of the 75k (75k indicates a moleculatr weight of 70,000 to 75,000) dna binding protein, an early virus-coded protein in adenovirus 2-infected kb cells, and its regulation were studied by using a radioimmune precipitation inhibition assay. the protein was first detected at 4 h postinfection and accumulated at an expoential rate. an arrest of further synthesis (accumulation) was observed at 10 to 11 h postinfection, coinciding with the onset of synthesis of late virion proteins. in contr ... | 1976 | 1271518 |
| [electronmicroscopic visualization of virusparticles in cases of gastroenteritis of infants and children (author's transl)]. | in 72 faecal extracts examined from babies and infants with an acute gastroenteritis, virus particles were found in 29 cases. these particles could be classified as reovirus-like in 16 cases, coronaviruses in 11 cases and adenoviruses in 2 cases. the electron microscopic investigation of 11 faecal extracts from healthy babies did not show any of these particles. comparative studies on the concentration methods were performed. | 1976 | 1271697 |
| [mitotic cycle in cell cultures infected with oncogenic type-12 adenoviruses]. | oncogenic adenovirus of type 12 in the culture of rat embryo fibroblasts, in which it induces an abortive infection, and in hep-2 culture, whereas it shows reproduction and causes lytic infection, in the early stationary phase causes shortening of the mitotic cycle and its separate periods. reduction of the mitotic cycle duration occurs on account of shortening of the presynthetic stage and dna synthesis stage. this property of the virus is in agreement with its capacity to stimulate proliferati ... | 1976 | 1274264 |
| analysis of tat transactivation of human immunodeficiency virus transcription in vitro. | the hiv tat protein is a potent transactivator of hiv transcription, increasing both rna initiation and elongation. we now demonstrate that purified, full-length 86 amino acid tat protein specifically transactivates the hiv ltr in vitro to a high level (25- to 60-fold). tat transactivation was specifically blocked by anti-tat serum, but not preimmune serum. tat did not transactivate transcription from the control adenovirus major late promoter (admlp). hiv transcription was blocked at various fu ... | 1992 | 1282057 |
| molecular cloning and restriction enzyme analysis of bovine adenovirus type 3. | bovine adenovirus type 3 (bav3) is a dna virus that causes respiratory and gastrointestinal disorders in cattle. the viral genome consists of a linear double-stranded dna molecule (35,000 base pairs) with inverted terminal repeats at each of its 5' molecular ends. we have subcloned 10 hindiii fragments spanning 4.9-96.0%, 5 ecori fragments spanning 3.4-89.5% and 2 xbai fragments spanning 35.7-82.9% of the bav3 (strain wbr-1) genome into the bacterial cloning vector puc19. the subcloning of the v ... | 1992 | 1302248 |
| multiple enlargements in the right inverted terminal repeat of the dna of canine adenovirus type 2. | the manhattan strain of canine adenovirus type 2 (cav 2) was examined. restriction endonuclease analysis and blot hybridization experiments revealed the heterogeneity of the viral dna. at least 9 unequally expanded species of the viral genome have been recognized. this diversity is caused by different enlargements in the right inverted terminal repeat (itr) of the virus. the differences between the individual enlargements were shown to be the different multiples of 150 base pairs. relatedness of ... | 1992 | 1307439 |
| strategies for the identification of icosahedral virus receptors. | | 1992 | 1309536 |
| hela cells grown continuously in protein-free medium: a novel model for the study of virus replication. | human carcinoma of the cervix cell line hela, adapted to continuous growth in a protein-free chemically defined medium, was used as substrate for the replication of several human pathogenic viruses. growth characteristics of the cells designated as hela-pf in protein-free 1:1 nutrient mixture of dulbecco's modified mem and ham's f-12 supplemented with l-ascorbic acid 2-phosphate were similar to those of the cells grown in a serum-supplemented medium. after 30 months (135 subcultures) in the prot ... | 1992 | 1310958 |
| the effect of cicloxolone sodium on the replication in cultured cells of adenovirus type 5, reovirus type 3, poliovirus type 1, two bunyaviruses and semliki forest virus. | the effect of cicloxolone sodium (ccx) on the replication of typical representatives of different virus families [adenovirus type 5 (ad-5), reovirus type 3 (reo-3), bunyamwera and germiston viruses, poliovirus type 1 (polio-1) and semliki forest virus (sfv)] in tissue culture was investigated. the golgi apparatus inhibitor monensin (mon) and ccx were shown to have analogous effects on some aspects of virus replication. although the mon-like effect of ccx played no role in the antiviral activity ... | 1992 | 1311362 |
| an ocular model of adenovirus type 5 infection in the nz rabbit. | ocular adenoviral infections occur worldwide, and currently, there is no ocular animal model for evaluating new antivirals or studying pathogenesis. with a paired-eye design, an ocular model was developed in 32 new zealand rabbits following topical and intrastromal inoculation with a clinical isolate of adenovirus type 5 (ad5 mcewen). clinical signs of infection--conjunctivitis, corneal edema, subepithelial infiltrates, and iritis--and seroconversion were evaluated. replicating virus on the ocul ... | 1992 | 1312070 |
| inhibition of poliovirus rna synthesis by brefeldin a. | brefeldin a (bfa), a fungal metabolite that blocks transport of newly synthesized proteins from the endoplasmic reticulum, was found to inhibit poliovirus replication 10(5)- to 10(6)-fold. bfa does not inhibit entry of poliovirus into the cell or translation of viral rna. poliovirus rna synthesis, however, is completely inhibited by bfa. a specific class of membranous vesicles, with which the poliovirus replication complex is physically associated, is known to proliferate in poliovirus-infected ... | 1992 | 1312615 |
| [virucidal effectiveness of some commercial products for chemothermal disinfection methods for temperature resistant viruses and bacteriophages--evaluation of a test model]. | in the laboratory assay of chemothermal virus disinfection procedures a test system is required which allows to differentiate between the physical effects of the temperature and the chemical effects by the disinfectant used. from the four test viruses recommended by the german association for the control of virus diseases (deutsche vereinigung zur bekämpfung der viruskrankheiten) only sv 40 tumor virus showed a sufficient thermal stability at 55 degrees c to take it in consideration as test viru ... | 1992 | 1313238 |
| driving adenovirus type 12-transformed balb/c mouse cells to express high levels of class i major histocompatibility complex proteins enhances, rather than abrogates, their tumorigenicity. | the tumorigenicity of adenovirus type 12 (ad12)-transformed cells has been attributed to the low levels of class i major histocompatibility complex (mhc) protein expression by these cells. these levels of class i proteins are thought to be below the threshold critical for cytotoxic t-lymphocyte recognition, a process that may be involved in tumor cell immunosurveillance. we have used gene transfer experiments to investigate the role played by class i protein expression in the tumorigenicity of a ... | 1992 | 1313906 |
| virus-associated rnas (va-i and va-ii). an efficient target for the detection of adenovirus infections by in situ hybridization. | the identification of adenovirus in tissue can be difficult. in situ hybridization for adenovirus nucleic acids may aid in the demonstration of adenovirus infections. to develop a probe against adenovirus, a 978 bp fragment of dna containing the va-i, va-ii, and a portion of the l-1 regions of the adenovirus type 2 genome was cloned into the sk+ vector. these regions were selected because they are generally conserved among adenoviruses and are abundantly transcribed during the lytic cycle. sense ... | 1992 | 1314030 |
| relationship between age of flock seroconversion to hemorrhagic enteritis virus and appearance of adenoviral inclusions in the spleen and renal tubule epithelia of turkeys. | a retrospective study was conducted to evaluate the temporal relationship between flock seroconversion to hemorrhagic enteritis virus (hev) and the appearance of adenoviral inclusions in the spleen and renal tubular epithelium. the study was conducted on samples of turkey poults submitted to the fresno branch of the california veterinary diagnostic laboratory system during may to december 1988. the study included 78 submissions (four to eight poults per submission) of ages ranging from 6 to 15 w ... | 1992 | 1314556 |
| globic: a very fast microcomputer program for fingerprinting, characterization and comparison of long nucleotide sequences. | this paper describes the program globic, which compares, characterizes and fingerprints even 0.1 mbase sequences in a few minutes with the aid of an ibm-at microcomputer. instead of the nucleotide sequences themselves, globic compares the local nucleotide or short oligonucleotide compositions. globic presents two-dimensional maps of contour lines depicting the similarity of two different sequences, a sequence compared to itself, to its complementary sequence or to a random sequence. a vocabulary ... | 1992 | 1314685 |
| in vitro transactivation of baculovirus early genes by nuclear extracts from autographa californica nuclear polyhedrosis virus-infected spodoptera frugiperda cells. | nuclear extracts, prepared from autographa californica nuclear polyhedrosis virus-infected spodoptera frugiperda cells during a time course of infection, were analyzed for activation of early gene transcription and for late gene transcription. the templates used in the in vitro transcription assays contained promoters for baculovirus genes that have been classified as immediate early, delayed early, and late. the promoters were derived from the baculovirus 39k, p26, gp64, and dna polymerase gene ... | 1992 | 1316463 |
| the 11,600-mw protein encoded by region e3 of adenovirus is expressed early but is greatly amplified at late stages of infection. | we have reported that an 11,600-mw (11.6k) protein is coded by region e3 of adenovirus. we have now prepared two new antipeptide antisera that have allowed us to characterize this protein further. the 11.6k protein migrates as multiple diffuse bands having apparent mws of about 14,000, 21,000, and 31,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. immunoblotting as well as virus mutants with deletions in the 11.6k gene were used to show that the various gel bands represent form ... | 1992 | 1316473 |
| chromatin structure, dna methylation, and gene expression at sites of viral integration in human fibroblasts. implications for chromosomal fragility. | we analyzed the structural and functional properties of a chromosomal region in which a recombinant hybrid virus adenovirus 5/sv40 preferentially integrates. our results demonstrated that the structure of the cellular targets for dna and rna viruses is very similar and that the cellular sequence flanking the integrated virus possesses, simultaneously, all the features postulated to be the molecular basis for chromosomal fragility. | 1992 | 1317251 |
| propagation of adenovirus types 40 and 41 in the plc/prf/5 primary liver carcinoma cell line. | the sensitivity of cell cultures to adenovirus types 40 and 41 (ad40/41) was compared by means of cell culture infectious dose (id50) assays using monolayer cultures in microtitre plates. the plc/prf/5 cell line derived from a primary human hepatocellular carcinoma was 100 times more sensitive to a laboratory strain of ad41, and 10 times more sensitive to a laboratory strain of ad40 and two ad41 stool isolates, than graham 293 and chang conjunctival cells commonly used for the propagation of the ... | 1992 | 1317878 |
| a serologic survey of the island fox (urocyon littoralis) on the channel islands, california. | the island fox is listed as a threatened species in california. a serologic survey of 194 island foxes (urocyon littoralis) was conducted over the entire range of the species on the channel islands (california, usa). antibody prevalence against canine adenovirus and canine parvovirus reached 97% and 59%, respectively, in some populations sampled. antibody prevalence of canine herpesvirus, canine coronavirus, leptospirosis and toxoplasmosis were low. antibodies against canine distemper virus were ... | 1992 | 1318424 |
| function and immunogenicity of human parainfluenza virus 3 glycoproteins expressed by recombinant adenoviruses. | human parainfluenza virus type 3 fusion (f) and hemagglutinin-neuraminidase (hn) cdna sequences were inserted into the e3 region of the adenovirus type 5 genome. cells infected with recombinant adenoviruses containing hpiv3 f (adf) and hn (adhn) sequences were shown to express hpiv3 f and hn proteins that were functional and immunogenic. the hn protein produced following adhn infection was glycosylated, expressed on the surface of infected cells and exhibited both hemagglutinin and neuraminidase ... | 1992 | 1320799 |
| detection of type ii avian adenoviral antigen in tissue sections using immunohistochemical staining. | an immunohistochemical staining technique for the detection of marble spleen disease (msd) viral antigens and other type ii avian adenoviral antigens was developed using a mixture of monoclonal antibodies produced against hemorrhagic enteritis (he) virus and a commercial streptavidin-biotin peroxidase indicator system. this technique was applied to both frozen and formalin-fixed paraffin-embedded tissue sections. the immunohistochemical staining technique was used on tissues from pheasants with ... | 1992 | 1320863 |
| immune function in pheasants experimentally infected with marble spleen disease virus. | two experiments were conducted to evaluate the effect of marble spleen disease virus (msdv) infection on the immune response of pheasants. in the first, 15 ring-necked pheasants were inoculated orally with cell-culture-propagated msdv and 15 received saline (controls). on days 7, 21, and 35 postinoculation (pi), all birds received sheep erythrocytes intravenously. hemagglutination titers to sheep erythrocytes were determined for serum samples collected weekly for 6 weeks. the virus-inoculated gr ... | 1992 | 1320870 |
| dependence of the adenovirus tripartite leader on the p220 subunit of eukaryotic initiation factor 4f during in vitro translation. effect of p220 cleavage by foot-and-mouth-disease-virus l-protease on in vitro translation. | the adenovirus tripartite leader (tpt) 5' untranslated region (5'utr) allows translation in poliovirus-infected cells, in which the p220 subunit of eukaryotic initiation factor 4f is degraded. this p220-independent translation was investigated by measuring in vitro translation in a reticulocyte lysate of a reporter gene, chloramphenicol acetyltransferase, coupled to the tpt 5'utr. the p220 subunit was degraded by translation of a foot-and-mouth-disease l-protease construct. surprisingly, the tpt ... | 1992 | 1321714 |
| an experimental animal model of adenovirus-induced ocular disease. the cotton rat. | the adenoviruses are a common cause of eye disease in humans and clinically cause three basic syndromes: epidemic keratoconjunctivitis, pharyngo-conjunctival fever, and nonspecific follicular conjunctivitis. although many serotypes of the adenovirus have been implicated, types 8, 19, and 37 are associated most commonly with ocular disease. there has not been a well-defined and reproducible animal model of this disease. the eyes of cotton rats inoculated with either adenovirus type 5 or type 8 de ... | 1992 | 1323254 |
| transforming growth factor beta 1 (tgf beta 1) reduces cellular levels of p34cdc2, and this effect is abrogated by adenovirus independently of the e1a-associated prb binding activity. | we have used e1a probes to study the roles of the p34cdc2 kinase and the retinoblastoma tumor susceptibility gene product (prb) in transforming growth factor beta 1 (tgf beta 1)-mediated growth suppression in mink lung epithelial (mv1lu) cells. in agreement with previous reports, we see a decline in p34cdc2 kinase activity and a loss of prb phosphorylation after tgf beta 1 treatment. we report here that tgf beta 1 induces not only a change in p34cdc2 kinase activity but a strong repression of p3 ... | 1992 | 1323350 |
| adenoviruses in the immunocompromised host. | adenoviruses are among the many pathogens and opportunistic agents that cause serious infection in the congenitally immunocompromised, in patients undergoing immunosuppressive treatment for organ and tissue transplants and for cancers, and in human immunodeficiency virus-infected patients. adenovirus infections in these patients tend to become disseminated and severe, and the serotypes involved are clustered according to the age of the patient and the nature of the immunosuppression. over 300 ad ... | 1992 | 1323383 |
| multimerization of icp0, a herpes simplex virus immediate-early protein. | icp0, a herpes simplex virus immediate-early gene product, is a highly phosphorylated nuclear protein that is a potent activator of virus and host genes. using biochemical and genetic assays employing plasmids encoding mutant forms of icp0 and a recombinant adenovirus that expresses icp0, we mutant forms of icp0 and a recombinant adenovirus that expresses icp0, we provide evidence that the protein multimerizes. some mutant forms of icp0 were transdominant and interfered with activation of a targ ... | 1992 | 1323713 |
| identification and characterization of a novel herpes simplex virus glycoprotein, gk, involved in cell fusion. | antipeptide sera were used to identify a novel glycoprotein encoded by the ul53 gene of herpes simplex virus type 1 (hsv-1). the ul53 gene product is thought to play a central role in regulating membrane fusion because mutations giving rise to the syncytial phenotype, wherein cells are extensively fused, frequently map to this gene. a single 40-kda protein, designated gk (the ninth hsv-1 glycoprotein to be described), was detected with antipeptide sera in cells infected with both wild-type and s ... | 1992 | 1323714 |
| viral susceptibility of an established cell line of swine embryo kidney. | the viral susceptibility of a cell line, named ksek6, newly established from the kidney cortex of swine embryo was tested for the indication of cpe occurrences and also plaque formations. the multiplication of porcine adenoviruses was considerably high in the cells among the virus strains tested though plaques of these viruses were hardly visible under agar overlay medium. two strains of swine enteroviruses, aujeszky's disease virus and hemagglutinating encephalomyelitis virus also multiplied we ... | 1992 | 1323743 |
| intravenous immunoglobulin: use in pediatric bone marrow transplantation. | | 1992 | 1324528 |
| detection of bovine adenovirus nucleic acid sequences in nasal specimens by biotinylated dna probes. | bovine adenovirus infection has been detected in experimentally infected lambs by applying the rapid and simple dna detection method of direct filter hybridization (dfh). three fragments of bav-2 dna were obtained by molecular cloning and labelled with biotin. clone 207, representing an ecori fragment between coordinates 35 to 55 of the viral genome, showed the highest sensitivity to detect adenovirus dna, thus, this probe was applied on nasal cells from the lambs. comparative tests revealed a c ... | 1992 | 1325721 |
| increased numbers of duodenal mucosal mast cells in turkeys inoculated with hemorrhagic enteritis virus. | the relation between average duodenal mast cell count, duodenal mucosal mast cell numbers, duodenal connective tissue mast cell numbers, circulating basophil numbers, heterophil-to-lymphocyte ratio, and lesion score were studied to gain an understanding of the events that may lead to intestinal lesion formation associated with hemorrhagic enteritis virus (hev) infection. changes in vascular permeability in the duodenum in birds inoculated with hev were examined, using colloidal carbon and ferrit ... | 1992 | 1326244 |
| outbreaks of egg drop syndrome due to eds-76 virus in quail (coturnix coturnix japonica). | two outbreaks of the egg drop syndrome were observed in quail flocks maintained on a farm together with chickens. the decrease in egg production ranged from 10.6 per cent to 50.6 per cent, and the number of soft-shelled eggs increased with the decline in egg production. in both the outbreaks haemagglutination inhibiting antibodies to eds-76 virus were detected. fluorescent viral antigen specific to eds-76 virus was also detected in the lining epithelial and glandular cells of the uterus. | 1992 | 1329304 |
| adenovirus containing a deletion of the early region 2a gene allows growth of adeno-associated virus with decreased efficiency. | efficient growth of adeno-associated virus (aav) requires helper functions provided by a coinfecting adenovirus or herpesvirus. earlier studies using adenoviruses having temperature-sensitive lesions in the early region 2a gene (e2a) produced contradictory evidence regarding the role of the e2a 72-kda dna-binding protein (dbp) in allowing efficient aav growth. these disparate results may reflect varying levels of residual function in the temperature-sensitive dbp. we examined this issue using an ... | 1992 | 1329332 |
| viral keratitis. | viruses that affect the cornea produce changes that range from benign, self-limited conjunctivitis to sight-threatening scarring and vascularization of the cornea. in this article, the forms of viral keratitis most commonly encountered by the clinician are reviewed. the epidemiology, clinical presentation, and treatment of infection by herpes simplex virus, varicella zoster virus, and the adenoviruses are discussed. also included are other viral infections of the cornea. | 1992 | 1334105 |
| the use of polymerase chain reaction for the detection of chlamydial keratoconjunctivitis. | ocular swabs from 30 consecutive patients with follicular conjunctivitis were screened for chlamydia trachomatis, herpes simplex virus, and adenovirus by a variety of laboratory techniques. for the detection of c. trachomatis, we compared two polymerase chain reaction methods, mccoy cell culture isolation, and the direct fluorescein-conjugated monoclonal antibody test. four of 30 patients tested positive for c. trachomatis by using both conventional and biotinylated polymerase chain reaction met ... | 1992 | 1334375 |
| tumor necrosis factor alpha stimulates expression of adenovirus early region 3 proteins: implications for viral persistence. | human adenovirus (ad) can cause persistent infections in humans. early region 3 (e3) of the virus appears to be implicated in this phenomenon. this transcription unit encodes proteins that interfere in various ways with host cell functions, including (i) cell-surface expression of histocompatibility class i antigens (hla), (ii) cell-surface expression of the epidermal growth factor receptor (egf-r), and (iii) the biological activity of tumor necrosis factor alpha (tnf-alpha). we transfected the ... | 1992 | 1334549 |
| pretreatment with topical 0.1% (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine inhibits adenovirus type 5 replication in the new zealand rabbit ocular model. | currently there is no clinically effective antiviral agent for the prevention or treatment of ocular adenoviral infections. using a paired-eye, masked design, we tested the antiviral efficacy of topical 0.1% (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine in the new zealand rabbit ocular model after topical and intrastromal inoculation with 100 microliters (4 x 10(5) plaque-forming units per eye) of adenovirus type 5 mcewen, a clinical isolate. prevention studies involved pretreatment (six t ... | 1992 | 1334824 |
| target recognition by bovine mononuclear, mhc-unrestricted cytotoxic cells. | a population of bovine non b/non t, cytotoxic lymphocytes with natural killer activity against virus-infected and non-infected embryonic kidney cells was functionally characterized. the data obtained in experiments of flow cytometry and immuno-peroxidase staining show that a cd2-, cd4-, cd8-, tcr gamma delta-, cd3+, cd45+, fcr+ lymphoid killer cell does exist within bovine peripheral blood leucocytes. this population can detect the down-regulation of class i mhc antigens or the expression of emb ... | 1992 | 1336249 |
| hemorrhagic enteritis virus inclusions in turkey renal tubular epithelium. | commercial turkeys from four iowa flocks, two illinois flocks, and three california flocks were submitted to state diagnostic laboratories because of a variety of health problems. the turkeys ranged in age from 5 to 12 weeks, included both hens and toms, and were owned by five different companies. some flocks had previously been immunized with live hemorrhagic enteritis vaccine, and other flocks were unvaccinated. in all accessions, basophilic intranuclear inclusion bodies were observed in renal ... | 1992 | 1336658 |
| reptilian viruses: adenovirus-like agent isolated from royal python (python regius). | an adenovirus-like agent was isolated from a moribund royal python (python regius). the dna containing virus replicated in igh2-cells at 30 degrees c forming eosinophilic intranuclear inclusion bodies. the virus proved to be stabile to treatment with chloroform, ph3 und ph 12 but it was labile to heat (56 degrees c). infected igh2 cells revealed symmetric hexagonal virus particles measuring 67-79 nm in the nucleus. the isolate shared characteristics with the viruses of the family adenoviridae. | 1992 | 1337233 |
| [serological study of the incidence of murine viruses in a population of small wild rodents (microtus pennsylvanicus ord, 1815)]. | the results of a serological survey of a free-living population of meadow voles (microtus pennsylvanicus) in pinawa, manitoba (canada) showed that these animals possessed antibodies to six of the eleven viruses tested for, namely: reovirus type 3, murine encephalomyelitis agent, ectromelia virus, murine adenovirus, murine hepatitis virus and lymphocytic choriomeningitis virus. the significant increase in the number of individuals possessing specific antibodies suggests that these viruses, or rel ... | 1992 | 1339064 |
| [detection of respiratory syncytial and adenovirus in nasopharyngeal aspirates: comparison of cellular cultures and immunofluorescence]. | respiratory syncytial and adenovirus are 2 of the most important pathogens for respiratory infection in infancy. we compared the results of cellular cultures and immunofluorescence in the detection of these agents in nasopharyngeal exudates from hospitalized infants. evidence for respiratory syncytial virus was searched in 776 samples. immunofluorescence was much more sensitive, detecting 303 positive results, compared to only 142 for cell culture. compared to immunofluorescence, the sensitivity ... | 1992 | 1340571 |
| pandora's box and the history of the respiratory viruses: a case study of serendipity in research. | as fastidious human parasites, the respiratory viruses other than influenza viruses have been among the last of the human viruses to be isolated. their recognition has been dependent upon the evolving technology of cell culture and equally upon a series of fortuitous observations by astute investigators. adenoviruses were discovered independently by two different groups of scientists, one utilizing explantation of ostensibly normal human tissues and the other recovering virus directly from epide ... | 1992 | 1342728 |
| latex agglutination and adenoviruses. i. detection of polyclonal antibodies by latex agglutination test. | a latex agglutination (la) test was developed for the detection and measurement of polyclonal anti-adenovirus antibodies. latex particles, coated with purified hexon, penton or fibre antigens displayed specific agglutination with 21 rabbit immune sera directed against the different antigens and the complete virus of 10 human and 2 animal adenovirus types. the sensitivity of la was compared to that of passive haemagglutination, complement fixation (cf) and immunodiffusion reactions. in experiment ... | 1992 | 1343944 |
| are vertebrate exons scanned during splice-site selection? | pairwise recognition of splice sites as a result of a scanning mechanism is an attractive model to explain the coordination of vertebrate splicing. such a mechanism would predict a polarity-of-site recognition in the scanned unit, but no evidence for a polarity gradient across introns has been found. we have suggested that the exon rather than the intron is the unit of recognition in vertebrates and that polyadenylation and splicing factors interact during recognition of 3'-terminal exons. inter ... | 1992 | 1359430 |
| immunoenhancement and suppression induced by adenovirus in chicken. | chickens injected intravenously (i.v.) with human adenovirus type 6 (ad6) reveal a 2-17-fold increase in the number of plaque-forming cells producing antibody (ab) against sheep red blood cells (srbc) 2-6 days after virus infection. further, polyclonal b-cell activation has been demonstrated by the quantitation of immunoglobulin-producing cells (igpc) and cells producing immunoglobulin (ig) of igm isotype (igpc mu) in the spleen of chicken inoculated with ad6. ad6 infection in chicken results in ... | 1992 | 1360755 |
| gene expression using dna viral vectors. | | 1990 | 1369360 |
| replication of adeno-associated virus type 2 in human lymphocytic cells and interaction with hiv-1. | adeno-associated virus (aav) is a nonpathogenic parvovirus which normally requires helper adenovirus or herpes-virus for replication. we examined the growth of aav type 2 in human lymphocytes and its possible interaction with hiv-1. three b cell lines (ck-b, hs-2, and uc729) and four t cell lines (molt-4, jurkat, hut78, and hut78+hiv, which is persistently infected with hiv-1) were infected with aav either in the presence or in the absence of adenovirus. aav dna was found in cells of all the lin ... | 1992 | 1372138 |
| map of cis-acting sequences that determine alternative pre-mrna processing in the e3 complex transcription unit of adenovirus. | the e3 complex transcription unit of adenovirus encodes four major mrnas (a, c, f, and h) and two minor (d and e) mrnas with overlapping exons, alternative splice sites, and two polyadenylation sites, termed e3a (upstream) and e3b (downstream). mrnas a and d use the e3a polyadenylation site, and mrnas c, e, f, and h use the e3b site. we have analyzed virus mutants with deletions throughout the e3 region in order to identify cis-acting sequences that function in e3 pre-mrna processing. the result ... | 1992 | 1388210 |
| high-efficiency gene transfer mediated by adenovirus coupled to dna-polylysine complexes. | employment of recombinant viruses as gene transfer vectors is limited by constraints on the size and functional design of the genetic material to be transferred as well as potential safety hazards deriving from obligatory co-transfer of viral genetic elements. as an alternative strategy that capitalizes on the efficient cellular entry mechanisms of viruses, we have derived adenovirus-polylysine-dna complexes whereby foreign dna is transferred bound to the exterior of the virion. this linkage was ... | 1992 | 1391034 |
| virus proteins that counteract host immune defenses. | | 1992 | 1394431 |
| synthesis and processing of the haemagglutinin-esterase glycoprotein of bovine coronavirus encoded in the e3 region of adenovirus. | the haemagglutinin-esterase gene (he) of bovine coronavirus (bcv) encodes a major viral membrane glycoprotein that elicits bcv-neutralizing antibodies. the bcv he gene was cloned into a human adenovirus serotype 5 (ad5) transfer vector in place of early transcription region 3, and a helper-independent recombinant virus was constructed by rescue of the transcription unit by homologous in vivo recombination between the vector and ad5 genomic dna. the bcv he polypeptide expressed by this recombinan ... | 1992 | 1402802 |
| coexpression of the simian immunodeficiency virus env and rev proteins by a recombinant human adenovirus host range mutant. | recombinant human adenoviruses (ads) that replicate in the intestinal tract offer a novel, yet practical, means of immunoprophylaxis against a wide variety of viral and bacterial pathogens. for some infectious agents such as human immunodeficiency virus (hiv), the potential for residual infectious material in vaccine preparations must be eliminated. therefore, recombinant human ads that express noninfectious hiv or other microbial proteins are attractive vaccine candidates. to test such an appro ... | 1992 | 1404612 |
| adenovirus transformation revertant resistant to retransformation by e1 but not by sv40-t and hpv16-e7 oncogenes. | we have previously described a revertant cell line which expresses a dominant tumor suppressor phenotype to e1 but not to heterologous oncogenes such as c-myc, n-ras, or polyoma middle t (sircar et al. (1988) oncogene 3, 725-728). dna tumor virus oncogenes have been suggested to transform cells via the common mechanism of sequestering the rb-105 antioncoprotein. this paradigm would predict that our revertant cell line, which is resistant to retransformation by e1a, should also be resistant to th ... | 1992 | 1413500 |
| multiple cis-acting dna elements that regulate transcription of the adenovirus 12 e1a gene. | to delineate cis-acting elements for adenovirus (ad) 12 e1a gene transcription, we transfected hela and nih3t3 cells with dnas having various deletions in the 5'-upstream region linked to the chloramphenicol acetyltransferase gene. deletions in the regions between nucleotide (nt) positions 54 and 166, and 167 and 200, with respect to the left end of the viral genome at nt position 1, caused a two- to three-fold reduction in transcription. transcription decreased to an almost undetectable level w ... | 1992 | 1413541 |
| [regulation of dna replication and gene expression of adenovirus]. | | 1992 | 1438828 |
| alterations in the levels of expression of specific cellular genes in adenovirus-infected and -transformed cells. | there is increasing evidence that changes in the transcriptional program of cellular genes in virus-transformed cells can contribute to virus transformation. it is, therefore, important to study altered expression patterns of cellular genes in adenovirus-infected and -transformed cells. we have used 40 different cellular genes or gene segments as hybridization probes to analyze the cytoplasmic rna from adenovirus type 2 (ad2)-infected kb cells, from ad5-transformed human cells (293) or from seve ... | 1992 | 1441738 |
| feasibility of adenovirus-mediated gene transfer in vivo. | we have evaluated the use of adenovirus as a new vector for gene transfer in vivo. the capacity of this virus to express a foreign gene even in the absence of viral and cellular replication makes it attractive to deliver a gene to quiescent cells. we show here that organs like lung, liver and muscles are relevant to this strategy. | 1992 | 1504658 |
| requirement for the adenovirus type 9 e4 region in production of mammary tumors. | oncogenic viruses demonstrating a strict tropism for the mammary gland provide special opportunities to study the susceptibility of this tissue to neoplasia. in rats, human adenovirus type 9 (ad9) elicits mammary fibroadenomas that are similar to common breast tumors in women, as well as phyllodes-like tumors and mammary sarcomas. by constructing recombinant adenoviruses between ad9 and ad26 (a related nontumorigenic virus), it was shown that the ad9 e4 region was absolutely required to produce ... | 1992 | 1519063 |
| mutations in two cysteine-histidine-rich clusters in adenovirus type 2 dna polymerase affect dna binding. | several point and linker insertion mutations in two cys-his-rich regions of adenovirus (ad) dna polymerase (pol) gene have been expressed in recombinant vaccinia virus. the resulting mutant enzymes were analyzed in vitro for their effects on dna synthesis activity, on ad-specific initiation assays, on gel shifts of ad origin sequences, and on interactions with adenovirus preterminal protein (ptp) and nuclear factor i (nfi). in general, mutants in downstream cys-his sequences had a pronounced eff ... | 1992 | 1527843 |
| adenovirus l1 52- and 55-kilodalton proteins are present within assembling virions and colocalize with nuclear structures distinct from replication centers. | analysis of a temperature-sensitive mutant, ad5ts369, had indicated that the adenovirus l1 52- and 55-kda proteins (52/55-kda proteins) are required for the assembly of infectious virions. by using monoclonal antibodies directed against bacterially produced l1 52-kda protein, the l1 52/55-kda proteins were found to be differentially phosphorylated forms of a single 48-kda polypeptide. both phosphoforms were shown to be present within all suspected virus assembly intermediates (empty capsids, 50 ... | 1992 | 1527852 |
| functional changes in temperature-sensitive mutants of the adenovirus single-stranded dna-binding protein are accompanied by structural alterations. | adenovirus requires the virus-encoded single-stranded dna-binding protein (dbp) to replicate its dna. we have previously shown (m. tsuji, p. c. van der vliet, and g. r. kitchingman, j. biol. chem. 266:16178-16187, 1991) that the inability of three temperature-sensitive (ts) mutant dbps (ad2+ nd1ts23, ad2ts111a, and ad5ts125) to support dna replication at the nonpermissive temperature was associated with impaired ability to bind to dna. in this study, we examined these mutant proteins for structu ... | 1992 | 1530772 |
| high-level expression of the tick-borne encephalitis virus ns1 protein by using an adenovirus-based vector: protection elicited in a murine model. | tick-borne encephalitis virus (tbev) encodes an abundant, highly immunogenic nonstructural glycoprotein, ns1. the function of this protein has yet to be determined. we have cloned the ns1 gene from the neudorfl strain of tbev under the control of the powerful constitutive cytomegalovirus major immediate-early promoter into an adenovirus e1 deletion mutant. the novel combination of the cytomegalovirus immediate-early promoter and the adenovirus vector produced extremely high levels of ns1 express ... | 1992 | 1532212 |
| simian virus 40 small t antigen trans activates the adenovirus e2a promoter by using mechanisms distinct from those used by adenovirus e1a. | as reported previously for simian virus 40 small t antigen, polyomavirus small t antigen stimulates transcription directed by the adenovirus e2a and va-i promoters during transient transfection assays. to determine whether papovaviral small t antigens might employ biochemical mechanisms during transcription activation that are either similar to or distinct from other viral trans activators, i compared the abilities of simian virus 40 small t antigen and adenovirus e1a to regulate the e2a promote ... | 1992 | 1532215 |
| adenovirus as an expression vector in muscle cells in vivo. | attempting gene transfer in muscle raises difficult problems: the nuclei of mature muscle fibers do not undergo division, thus excluding strategies involving replicative integration of exogenous dna. as adenovirus has been reported to be an efficient vector for the transfer of an enzyme encoding gene in mice, we decided to explore its potential for muscle cells. advantages of adenovirus vectors are their independence of host cell replication, broad host range, and potential capacity for large fo ... | 1992 | 1557362 |
| evaluation of a subunit vaccine for bovine adenovirus type 3. | the hexon subunit of bovine adenovirus serotype 3 (bav-3) was purified by use of anion-exchange chromatography. a vaccine composed of the purified hexon in immune-stimulating complexes was administered and induced high titer of virus-neutralizing antibody in rabbits and calves. | 1992 | 1575381 |
| acceleration of adenovirus replication and increased virion production by treatment with the steroid hormone 17 beta-estradiol. | we report here that concentration of an estrogen known to promote enhanced transformation and to increase oncogenicity of rat embryo cells, accelerate the production and increase the yield of progeny virions in adenovirus type 12 (ad 12)-infected hep-2 cells. further, measurement of the incorporation of radioactive rna and dna precursors indicated that macromolecular synthesis in the estrogen-treated, infected cells was accelerated. possible explanations for this observation are discussed. | 1992 | 1584077 |
| effects of two adenoviruses (type 1 and type 8) on functional properties of bovine alveolar macrophages in vitro. | cultures of bovine alveolar macrophages were inoculated with type-1 and type-8 adenoviruses, initially isolated from calves with respiratory tract disease, and functional properties of the cells were observed over a period of 10 to 11 days. both viruses replicated in macrophages; viral titers were low (less than 3.75 log10 tcid50/0.1 ml), and intranuclear inclusions were detected by indirect immunofluorescence in 5 to 10% of the cells from 3 days after inoculation. highest titers were induced by ... | 1992 | 1626769 |
| an adenoviral vector system for functional identification of nuclear receptor ligands. | a recombinant adenovirus system has been designed that confers glucocorticoid responsiveness upon infected cells in culture. two mutually dependent viruses are required: a trans-activator virus containing the human glucocorticoid receptor transcription unit and a second receptor virus harboring a glucocorticoid response element linked to the firefly luciferase gene. another reciprocal pair of viruses has been generated; one member expresses the rat thyroid hormone receptor alpha, while the other ... | 1991 | 1645457 |
| sero-prevalences of selected cattle diseases in the kafue flats of zambia. | sera from five traditionally managed herds grazing in the kafue flats were tested for antibodies to bovine viral diarrhoea-mucosal disease (bvd-md), parainfluenza 3 (pi3), infectious bovine rhinotracheitis-infectious pustular vulvovaginitis (ibr-ipv), bovine adenovirus 3 (bav3) and bluetongue (bt). the sero-prevalences of the first four diseases were respectively 76.2, 94.4, 42.1 and 87.4%. five samples (2.3%) gave doubtful reactions for bt. prevalences of 28.5% for brucellosis, 14% for rift val ... | 1991 | 1646515 |
| viral encephalitis. | nonherpetic encephalitis outside the newborn period is usually a self-limited disease. the majority of patients will recover without significant sequelae, and require only supportive therapy during the acute illness. though the underlying viral etiology frequently will escape detection, identification of the infecting agent has considerable prognostic value which can complement clinical measures of severity of disease. the most important initial task of the clinician faced with a case of presump ... | 1991 | 1647166 |
| viral respiratory disease of the horse. | the diagnosis of any viral respiratory disease relies on laboratory procedures to isolate the virus and demonstrate a significant rise in serum antibody titers. to isolate viruses from the upper respiratory tract, it is imperative that nasopharyngeal swabs are obtained from animals in the early acute stage of illness, i.e., during the pyrexic phase when the virus is replicating. nasopharyngeal swabs must be placed in a virus transport medium and forwarded immediately to the laboratory at refrige ... | 1991 | 1647261 |
| viral gastroenteritis. | | 1991 | 1647494 |
| synergism between aphidicolin and adenoviruses in the induction of breaks at fragile sites on human chromosomes. | infection of human embryonic kidney cells with adenoviruses results in the induction of gaps and breaks in cell chromosomes. with adenovirus type 12, cytogenetic damage is known to occur primarily at fragile sites on chromosomes 1 and 17. we have mapped adenovirus type 5-induced breaks and have observed that, although they occur on all chromosomes, they are localized primarily on bands where fragile sites have been mapped. the susceptibility of fragile sites to adenovirus led us to investigate t ... | 1991 | 1648440 |
| viral pneumonitis. | viral pneumonitis can affect all age groups and normal as well as compromised hosts. this article discusses salient features of pneumonitis caused by respiratory syncytial virus, adenoviruses, varicella-zoster virus, herpes simplex virus, influenza a and b viruses, and cytomegalovirus. the clinical picture, diagnosis, treatment and prevention for each agent are discussed. | 1991 | 1649731 |
| acute respiratory viral infections in ambulatory children of urban northeast brazil. | the morbidity of acute respiratory infections in young children and the role of respiratory viruses were evaluated in a 29-month household-based study in an impoverished urban population in fortaleza, brazil; subjects were 175 children less than 5 years of age in 63 families. home visits were conducted three times weekly during which staff recorded the presence of respiratory and systemic symptoms and collected upper respiratory tract samples for viral isolation. a large and sustained burden of ... | 1991 | 1649871 |
| susceptibility of normal human joint tissue to viruses. | a model system has been developed to investigate the comparative ability of different viruses to replicate and persist intraarticularly. the viruses chosen for study were rubella, mumps, coxsackie b4, adenovirus and varicella zoster, a selection of viruses with different degrees of association with joint symptoms in clinical studies. our results showed that these viruses demonstrated a range of abilities to infect and persist in human joint tissue cultured in vitro. the most arthritogenic viruse ... | 1991 | 1650840 |
| [an adenovirus epidemic in patients with kidney transplantation]. | during weekly routine virological screening of kidney transplant patients 12 out of 15 patients within a period of four months were found to be infected with adenovirus. all isolates were of the same serotype, type adii + 35/hii. however, dna restriction enzyme analyses showed the presence of two different dna variants which were associated with three different epidemiological episodes. the epidemic probably started with reactivation of latent virus in a limited number of patients, after which i ... | 1991 | 1650922 |