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comparative pharmacological studies of n-(2-benzhydryloxyethyl)homomorpholine (linadryl h), linadryl and benadryl. i. antihistaminic, anticholinergic and spasmolytic effects of linadryl h. 197720737
local anesthetic contracture and relaxation of airway smooth muscle. 197720826
demonstration of beta-lipotropin activating enzyme in porcine pituitary. 197720892
mitochondrial ca2+ metabolism in aortic smooth muscle of swine. effect of mild hypercholesterolemia. 197720901
porcine malignant hyperthermia. v: fatal hyperthermia in the pietrain pig, associated with the infusion of alpha-adrenergic agonists.the effects of the administration of noradrenaline alone, and noradrenaline with either phentolamine or propranolol, on thermogenesis and substrate mobilization were investigated in six pietrain (mh-susceptible) and six large white (unsusceptible) pigs. the infusion of noradrenaline alone produced a significantly increased lipolytic response and a significantly decreased hyperglycaemic response in pietrain pigs compared with the large white breed. although noradrenaline alone produced only a sma ...197720913
interactions of tetrahymena dynein with microtubule protein. tubulin-induced stimulation of dynein atpase activity.the atpase (ec 3.6.1.3) activity of 30 s dynein from tetrahymena cilia was remarkably stimulated by porcine brain tubulin at ph 10. the activity increased with increasing concentration of tubulin until the molar ratio of tubulin dimer to 30 s dynein reached approx. 10. the optimum of the atpase activity of 30 s dynein in the presence of tubulin was 1-2 mm for mgcl2 and 2 mm for cacl2. increasing ionic strength gradually inhibited the stimulation effects of tubulin. activation energies of 30 s dy ...197720950
circular dichroism of porcine, bovine, and equine pancreatic phospholipases a2 and their zymogens. unusual conformations simulating helix content. 197720973
immunoelectrophoretic studies on pig intestinal brush border proteins.brush borders were prepared from pig intestinal mucosa and the membrane proteins solubilized with either triton x-100 or papain. proteins, thus released, were used as antigens to raise antisera in rabbits. the immunoglobulin g fractions were isolated and shown by the double layer immunofluorescence staining technique to react only with the brush border region of the enterocyte. the antibodies obtained were used in immunoelectrophoretic studies on the brush border proteins. eight hydrolytic activ ...197720974
effect of tyrosine ionization upon biological activities of angiotensin ii and two new peptide analogues.the role of the tyrosine side-chain in the smooth muscle contracting activity of angiotensin iii was investigated by determining intrinsic activities and ed50 values of 4-(3-chlorotyrosine)angiotensin ii and 4-(3-benzyltyrosine)angiotensin ii in the isolated guinea-pig ileum and rat uterus. 4-(3-chlorotyrosine)angiotensin ii activity was compared with that of angiotensin ii at different ph values, in which the ratio of their degrees of phenolic ionization varied. the results indicated that depro ...197720979
alterations in temperature and histamine receptor function. 197721104
ganglion blocking activity of a series of semisynthetic quaternary pyrrolizidines. 197721136
enumeration of t rosette-forming cell population in rabbit peripheral blood using human, sheep, guinea-pig, rat, mouse and chicken red blood cells under different experimental conditions.attempts were made to estimate peripheral t-lymphocytes in rabbits by their ability to form rosettes with sheep, human, guinea-pig, rat, mouse and chicken rbc. the reaction was studied under different conditions of ph and temperature. the test was also carried out in the presence or absence of glutaraldehyde. with sheep rbc, lymphocytes from 2 of the 5 rabbits studied formed rosettes to the extent of 1.5% in the system containing glutaraldehyde. these results however were not reproducible when s ...197721220
evidence from agonist and antagonist studies to suggest that the beta1-adrenoceptors subserving the positive inotropic and chronotropic responses of the heart do not belong to two separate subgroups.the positive inotropic and chronotropic responses of guinea-pig isolated left and right atria to 17 sympathomimetic amines were examined under conditions selected to control the pharmacological environment. each agonist was compared with (--)-isoprenaline as the reference by constructing dose-response curves. equiactive molar concentration ratios relative to (--)-isoprenaline were calculated at the ec50 for rate and tension responses. statistical analysis revealed that (--)-noradrenaline and (+/ ...197721238
effects of dantrolene sodium on isolated skeletal, smooth and cardiac muscle of the guinea-pig. 197721244
effect of tazolol on beta-adrenoceptors in isolated preparations of the guinea-pig and rat. 197721245
possible mechanism of action of beta-phenethylamine in migraine. 197721252
influence of pyridylisatogen tosylate on contractions produced by atp and by purinergic stimulation in the terminal ileum of the guinea-pig.2-2'pyridylisatogen tosylate (pit), which antagonizes the inhibitory action of atp in the taenia caeci, did not antagonize the excitatory effects of exogenous atp and of purinergic stimulation of the terminal guinea-pig ileum. pit (0-5--2-5 mum) potentiated the atp-induced contractions and also the contractions produced by potassium chloride, though the potentiation could not be related to the dose in every experiment. the responses to noradrenaline, adrenaline and histamine were slightly inhibi ...197721258
analysis of the beta-receptor mediated effect on slow-contracting skeletal muscle in vitro.subtetanic contractions of the guinea-pig isolated soleus, a slow-contracting skeletal muscle, were evoked by transmural field-stimulation. isoprenaline caused a dose-dependent depression of the contractions. this effect was inhibited by propranolol and h 35/25 (1-(p-tolyl-2-isopropylamino-1-propanol) but not by practolol. similar results were obtained for terbutaline. tazolol and h 80/62 (1-isopropylamino-3-(p-hydroxyphenoxy)-2-propanol (hcl), selective beta1-agonists, had no effect per se but ...197721260
effects of some drugs on the circular muscle of the isolated lower oesophagus. 197721262
modification of pig heart lactate dehydrogenase with methyl methanethiosulphonate to produce an enzyme with altered catalytic activity.methyl methanethiosulphonate was used to produce a modification of the essential thiol group in lactate dehydrogenase which leaves the enzyme catalytically active. methyl methanethiosulphonate produced a progressive inhibition of enzyme activity, with 2mm-pyruvate and 0.14mm-nadh as substrates, which ceased once the enzyme had lost 70-90% of its activity. in contrast, with 10mm-lactate and 0.4mm-nad+ as substrates the enzyme was virtually completely inhibited. the observed inhibition was critica ...197715552
porcine a blood group-specific n-acetylgalactosaminyltransferase.porcine a blood group-specific n-acetylgalactosaminyl-transferase required either mn2+, cd2+, or zn2+ for activity and 2'-o-alpha-fucosylgalactosides as acceptor substrates. the presence of detergent stabilizes the enzyme but is not essential for catalysis. to obtain information about the kinetic mechanism of the transferase reaction, initial rate parameters have been determined using 2'-fucosyllactose or a--mucin as acceptors, and mn2+ or cd2+ as cosubstrates. 2'-fucosyllactose is a competitive ...197714963
porcine a blood group-specific n-acetylgalactosaminyltransferase. i. purification from porcine submaxillary glands.the membrane-bound n-acetylgalactosaminyltransferase from porcine submaxillary glands which provides a blood group specificity to mucin has been purified 38,000-fold by affinity chromatography on udp-hesanolamine-agarose in aqueous triton x-100. design of a suitable purification procedure was developed by assessing the strength of interaction between enzyme and affinity adsorbent using batch desorption. the pure transferase has an apparent molecular weight of 100,000 as judged by zonal centrifug ...197714962
mathematical analysis of ligand-induced monomerization and dimerization in the monomer dimer equilibrium of proteins. application to d-amino acid oxidase.we have mathematically analyzed ligand-induced monomerization and dimerization in a protein monomer-dimer equilibrium system, in which the monomer has one and the dimer two binding sites. these dimer sites have the same binding constants for the first ligand but may cooperatively interact when one of them is occupied by a ligand molecule. in this system, the apparent dimerization constant and the apparent molecular weight are functions of free ligand concentration, and depend on the intrinsic bi ...197714929
influence of ph and sodium on the inhibition of guinea-pig heart (na+ + k+)-atpase by calcium.the inhibition of guinea-pig heart (na+ + k+)-atpase (atp phosphohydrolase ec 3.6.1.3) by calcium has been studied at ph 7.4, 6.8 and 6.4. 1. a decrease in ph reduced the threshold inhibitory concentration of calcium and the calcium concentration producing an inhibition of 50% of the enzyme activity. 2. calcium reduced the apparent affinity of the enzyme of na+, this effect occurred only at ph 7.4. 3. calcium increased the apparent affinity of the enzyme for k+, this effect was enhanced at acidi ...197714690
conformational transition accompanying the binding of ca2+ to the protein activator of 3',5'-cyclic adenosine monophosphate phosphodiesterase.the ca2+-dependent protein activator of 3':5'-cyclic adenosine monophosphate phosphodiesterase is shown to undergo a conformational transition upon binding of 2 mol of ca2+/mol of activator. circular dichroic studies indicate that ca2+ induces an increase of 5-8% in alpha-helix content with a concomitant decrease in the amount of random coil. in the absence of ca2+ and in the presence of ethylenebis(oxoethylenenitrilo)tetraacetic acid (egta), the protein contains 30-35% alpha helix, 50% random c ...197714663
preparation of coenzymic activity of soluble polyethyleneimine-bound nadp+ derivatives.alkylation at n-1 of the nadp+ adenine ring with 3,4-epoxybutanoic acid gave 1-(2-hydroxy-3-carboxypropyl)-nadp+. enzymic reduction of the latter, followed by alkaline dimroth rearrangement and enzymic reoxidation, gave n6-(2-hydroxy-3-carboxypropyl)-nadp+. on the other hand, bromination at c-8 of the nadp+ adenine ring, followed by reaction with the disodium salt of 3-mercaptroproionic acid, gave 8-(2-carboxyethylthio)-nadp+. carbodimide coupling of the three carboxylic nadp+ derivatives to pol ...197713999
interactions between the effects of alpha- and beta-adrenoceptor agonists and adenine nucleotides on the membrane potential of cells in guinea-pig liver slices.1 the beta-adrenoceptor agonist isoprenaline normally causes only a small and inconsistent increase in the membrane potential of cells in guinea-pig liver slices, in contrast to the large hyperpolarizations seen with alpha-agonists. however, after a selective alpha-adrenoceptor agonist has been applied, the response to isoprenaline becomes greatly enhanced. 2 simultaneous application of small doses of an alpha- and beta-agonist produce hyperpolarizations larger than the sum of the responses to e ...197713900
solubilised intrinsic factor receptor from pig ileum and its characteristics.the vitamin b-12-intrinsic factor receptor was shown to be present in pig ileum and localized on the brush borders of the enterocytes, and to be solubilisable with triton x-100. at neutral ph and in the presence of ca2+ it bound the vitamin b-12-intrinsic factor but not the vitamin b-12-cobalophilin complex. the solubilised vitamin b-12-intrinsic factor-receptor complex consisted of two molecular species with clear stokes radii (13.11 and 33.53 nm), sedimentation coefficients (15.1 and 45.1 s) a ...197713861
the dissociation of flavin coenzymes from trypsin-solubilized nadph/cytochrome c (p-450) reductase of pig-liver microsomes.the change in fluorescence emission at 520 nm after excitation at 365 nm was used to investigate the effect of ph and ionic strength on the dissociation of flavin cofactors from microsomal nadph/cytochrome c (p-450) reductase. in the unmodified enzyme both the fad and fmn moieties appeared to dissociate at a similar rate and followed first-order kinetics. the rate constant for the dissociation was increased by low ph and high ionic strength, particularly in the range ph 4.4-3.8 (0.02 m acetate b ...197612969
kinetics of the interaction between aspartic aminotransferase and anions.the kinetics of the interaction between deionized supernatant aspartic aminotransferase and various anions (cacodylate, phosphate and chloride) were studied by the temperature-jump technique. the anion concentration in the range covered by our experiments does not affect the transamination rate. on the other hand the conformational transition, recently observed at the active site of the enzyme, is hindered by an excess of anions. a single relaxation effect was observed at the enzyme chromophore ...197612967
stopped-flow spectrophotometric characterization of enzymic reaction intermediates in the anaerobic reduction of pig-plasma benzylamine oxidase by amine substrates.reduction of benzylamine oxidase by p-methoxybenzylamine under anaerobic conditions leads to biphasic absorbance changes at 470 nm. these reflect the intermediate formation of an enzyme substrate complex with spectral properties different from those of native enzyme and fully reduced enzyme. the spectrally modified enzyme-substrate complex exhibits a broad difference absorption band centered around 360 nm. the transient accumulation of this intermediate during reaction can be conveniently follow ...197612963
structure-function relationships in tpn-dependent isocitrate dehydrogenase. ii. determination of the paramagnetic relaxation rates of water protons in complexes of enzyme, mn(ii), substrates, cofactors, and inhibitors. 197719045
structure-function relationships in tpn-dependent isocitrate dehydrogenase. i. electron paramagnetic resonance studies of the interaction of enzyme-bound mn(ii) with substrates, cofactors, and substrate analogues.electron paramagnetic resonance (epr) spectra were obtained for various isocitrate dehydrogenase-mn(ii) complexes. the qualitative effects of the binding of substrates, nucleotides, and substrate analogues on the isotropic character of the electronic environment of enzyme-bound mn(ii) were subsequently investigated. the addition of isocitrate produces a markedly anisotropic spectrum whereas alpha-ketoglutarate does not alter the spectrum of enzyme-mn(ii) substantially. this suggests direct coord ...197719044
redox properties of microsomal reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase and cytochrome b5.hepatic nadh-cytochrome b5 reductase was reduced by 1 mol of dithionite or nadh per mol of enzyme-bound fad, without forming a stable semiquinone or intermediate during the titrations. however, the addition of nad+ to the partially reduced enzyme or illumination in the presence of both nad+ and edta yielded a new intermediate. the intermediate had an absorption band at 375 nm and the optical spectrum resembled anionic semiquinones seen on reduction of other flavin enzymes. electron paramagnetic ...197719038
purification and properties of a new testosterone 17beta-dehydrogenase (nadp+) from guinea-pig liver.as a result of studies of guinea-pig live testosterone 17beta-dehydrogenase (nadp+) (ec 1.1.1.64), a new testosterone 17beta-dehydrogenase was discovered. the new enzyme was purified to a single homogeneous protein from the 105 000 g-supernatant fraction of guinea-pig liver by (nh4)2so4 fractional precipitation and two gel-filtration stages, deae-cellulose column chromatography and hydroxyapatite column chromatography. it was characterized by many properties. the enzyme has almost the same prope ...197718133
effect of ph on the interaction of benzoate and d-amino acid oxidase.the kinetic and equilibrium dissociation constants of the reversible binding of benzoate to hog kidney d-amino acid oxidase (daao) were studied at 19 degrees c over the ph range 5.3-10.5 by means of a stopped-flow apparatus and spectrophotometric titrations. a simple bimolecular reaction of the form second order-first order was observed; a two-step reaction was seen. analysis of the ph dependence of the bimolecular rate constants and equilibrium dissociation constants is consistent with three io ...197719047
the role of arginine in the triphosphopyridine nucleotide dependent isocitrate dehydrogenase of pig heart. 197719048
mechanism of refolding and reactivation of lactic dehydrogenase from pig heart after dissociation in various solvent media. 197719049
cyclic amp-dependent protein kinase phosphorylation of cardiac (na+ + k+)-atpases. effect on calcium binding.1. calcium binding to (na+ + k+)-atpase (atp phosphohydrolase, ec 3.6.1.3) preparations from beef and pig heart preparations of varying degrees of purity was measured. 2. binding was inhibited by mg2+, na+ and k+. inhibition by na+ and k+ appeared to be due to an ionic strength effect. 3. four classes of binding sites were identified with kd values for calcium of about 0.03, 1, 15 and 200 micrometer. 4. cyclic amp-dependent phosphorylation of the enzyme by protein kinase (atp: protamine o-phosph ...197719066
enhanced heat, alkaline and tryptic stability of acetamidinated pig heart lactate dehydrogenase.modification of 17 from 24 lysine residues in pig heart lactate dehydrogenase (l-lactate: nad+ oxidoreductase, ec 1.1.1.27) with methyl aceimidate yields an enzyme derivative with enhanced stability toward meat and alkaline denaturation as well as tryptic digestion. the specific activity of the modified enzyme is only slightly reduced197719075
reconstitution and lipid requirements of porcine tissue thromboplastin. 197719079
immobilised lipoamide dehydrogenase. 1. preparation and properties of the enzyme immobilised by disulphide interchange. 197719245
the role of divalent cations in the activation of the nadp+-specific isocitrate dehydrogenase from pisum sativum l.a divalent cation electrode was used to measure the stability constants (association constants) for the magnesium and manganese complexes of the substrates for the nadp+-specific isocitrate dehydrogenase (ec 1.1.1.42) from pea stems. at an ionic strength of 26.5 mm and at ph 7.4 the stability constants for the mg2+-isocitrate and mg2+-nadp+ complexes were 0.85 +/- 0.2 and 0.43 +/- 0.04 mm-1 respectively and for the mn2+-isocitrate and mn2+-nadp+ complexes they were 1.25 +/- 0.07 and 0.75 +/- 0.0 ...197720207
prolonged safe aortic clamping by combining membrane stabilization, multidose cardioplegia, and appropriate ph reperfusion. 197721327
lipogenic enzyme activity in relation to fat accumulation. 197721364
confirmation of conductance increase by adrenalin in the guinea-pig taenia coli (alpha-action). 197721402
experimental study on the h1 and h2 histamine receptors. 197721423
the potentiating effect of alpha-adrenergic blocking drug on the action of bronchodilators (author's transl). 197721431
sensitization of contracted tracheal smooth muscle to beta-adrenergic spasmolysis by subthreshold doses of papaverine.in certain cases beta-adrenergic substances cause little or no relaxation of tracheal or bronchiolar smooth muscles contracted by spasmogenic mediators. this phenomenon is explained on the basis of the model of functional antagonism, with the suppositions that the spasmogenic system has a reserve and the spasmogen concentrations are much higher than the concentration which would cause a just-maximal contraction. if this is correct, the model predicts that it should be possible to restore the eff ...197721448
a rapid fluorometric assay of angiotensin-converting enzyme.a rapid, simple and sensitive method is described for the fluorometric assay of angiotesin-converting enzyme using fluorescamine. the critical factors such as optimal ph, incubation time, chloride ion, and inactivation by edta and 8-hydroxyquinoline were examined. the km value for hippuryl-l-histidyl-l-leucine was 0.5 mm. this method was applied to the assay of angiotensin-converting enzyme in the rat serum and the reproducible values were obtained with a 10 mul of the rat serum.197721470
anti-arrhythmic, antianginal and cardiovascular effects of the beta receptor blocking agent te-176 (chinoin-103). 197721516
histometrical aspects of testicular ontogenesis in the normal and unilateral cryptorchid pig proceedings. 197721520
cholesterol ester: lysolecithin acyltransferase activity of the aorta. 197721525
participation of phospholipids in arterial metabolism of cholesterol esters. 197721526
metabolism of phosphatidic acid and phosphatidylinositol in relation to transmitter release from synaptosomes. 197721530
homocarnosinase: a hog kidney dipeptidase with a broader specificity than carnosinase. 197721630
acid-base status in the blood of domestic pigs during exhausting locomotor stress of small and medium intensity.twenty-two fattening pigs were stressed until their rectal temperatures had reached 42 degrees c. the means of stress included walking on moving conveyor belt at ambient temperatures between 22 degrees c and 27 degrees c and relative humidities between 69 and 98%, belt speeds of 0.7 m/s, 1.3 m/s or 0.7 m/s, and stimulation by means of a forcing rod. rectal temperature, respiratory frequency, lactic acid level in blood plasma, ph, as well as pco2 and base excess in the blood were measured prior t ...197721642
sila analogs of meflophenhydramine. 197721643
effect of substrates and effectors on the reversible inactivation of pig spleen phosphofructokinase by adenosine triphosphate.1. to investigate the mechanism of the reversible inactivation of pig spleen phosphofructokinase by atp, the effect of order of addition of reactants (substrates, effectors and enzyme solution) was studied by preincubating the enzyme before assay with various combinations of its substrates and effectors. 2. preincubation of the enzyme with mgatp or atp at ph7.0 before addition of fructose 6-phosphate caused a rapid and much greater inhibition of activity than that observed when the reaction (car ...197721659
effect of dihydroergotoxine on activities of beta-receptor stimulators.the effect of dihydroergotoxine (det), an alpha-receptor blocker, on the bronchodilating effect of various beta-receptor stimulators was examined using the isolated guinea-pig tracheal smooth muscle. relaxation of isolated guinea-pig tracheal smooth muscle with isoproterenol hydrochloride, salbutamol hemisulfate and clorprenaline hydrochloride, was markedly increased by continous infusion of det. this result may suggest that det, an alpha-receptor blocker, can be used together with bronchodilato ...197721668
histomorphometric aspects of testicular morphogenesis in the naturally unilateral cryptorchid pig. 197721707
changes in serum lh concentrations during normal and abnormal sexual development in the pig.serum levels of luteinizing hormone (lh) were determined in fetal and prepubertal pigs from day 49 postcoitum to 25 weeks of age, and also in unilaterally cryptorchid, freemartin and castrated pigs of similar ages. serum lh was undetectable in the second trimester of pregnancy, but then gradually increased up to 2 weeks after birth in both sexes. 2-week-old pigs showed irregular lh peaks exceeding 2 ng/ml. serum lh concentrations decreased during the 3rd and 4th weeks of life. mean lh concen ...197721708
studies on a new beta-adrenoceptor blocker, 6-(2-hydroxy-3-isopropylamino)propoxy benzothiazole succinate (kf-577). 197721752
the use of liver transglutaminase for protein labeling. 197721793
release of spasmogens from rat isolated lungs by tryptamines.tryptamine and 5-hydroxytryptamine (5-ht) infused through the pulmonary circulation of rat isolated lungs released a spasmogen resembling slow reacting substance of anaphylaxis which we have denoted srs-t and a pge-like activity. srs-t was not extractable from krebs solution by several organic solvents at neutral or acid ph. it is therefore unlike other types of srs activity. the pge-like release had a threshold at about 2 microgram/ml of tryptamine or 5-ht and did not increase with increasing d ...197721802
isolation of ph-optima and apparent michaelis constants of highly purified enzymes from human and animal sources. comparison of enzymes of human and animal origin, i.enzymes from animal sources are frequently used for quality control of enzyme activity determination in clinical chemistry. for this purpose they should be very similar to human enzymes. it is shown that preparations of enzymes of diagnostic interest from human and porcine organs, purified in exactly the same way, have very similar ph-optima and apparent michaelis constants for their substrates.197721934
indolizine derivativatives with biological activity iii: 3-(3-aminopropyl)-2-methylindolizine, 3-(3-aminopropyl)-2-methyl-5,6,7,8-tetrahydroindolizine, and their n -alkyl derivatives.the syntheses and a preliminary pharmacological evaluation of some aminopropylindolizines and aminopropyltetrahydroindolizines are reported. all compounds showed anti-5-hydroxytryptamine, anti-histamine, and antiacetylcholine activities. some also exhibited weak cns activity.197721953
a benzoaquinolizine derivative with a neuroleptic-like action on cerebral monoamine turnover. 197721955
establishment of the hosts of bloodsucking mosquitoes in abkhazia. 3. fertility of females of mass mosquito species feeding on warm-blooded and cold-blooded animals. 197722029
amino acid transmitters (author's transl). 197722097
induction of the enzymes related to neurogenic amines (author's transl). 197722098
cyclic nucleotides in brain cells (author's transl). 197722099
current aspects of the study of stomach ulcer in swine. 197722156
resting metabolism of lactate in starved and fed pigs. 197722176
modification of mucus in animal models of disease. 197722224
the distribution of enzymes in the epidermis of the domestic cat.with the help of enzyme histochemical methods, the distribution and activities of several oxidative and hydrolytic enzymes in the epidermis of the densely-haired domestic cat have been studied. distinct oxidative enzyme activity could be demonstrated in all epidermal layers except the stratum corneum. among the hydrolytic enzymes investigated, strong reactions for non-specific esterases were visible especially in the str. granulosum and the basal lamellae of the str. corneum. positive reactions ...197722312
investigation of the bronchoconstriction induced by beta-adrenoceptor blocking drugs in guinea-pigs and rats proceeding. 197722371
neurotransmitter-sensitive adenylate cyclase in the hypothalami of guinea-pig, rat and monkey. 197722390
sex-limited and sex-modified genetic defects in swine--cryptorchidism.review of published data suggests that cryptorchidism in chester whites and yorkshires is caused by completely penetrant, recessive genes at two autosomal loci; in lacombes, multifactorial modes of inheritance are more plausible. different genetic systems control presence of the trait vs. number of sides affected; left testes are retained more often in almost all samples. contrary to previous claims prenatal viability of homozygous males and females is normal. genes causing cryptorchidism are un ...197722396
purinergic transmission. 197722431
characterization of the adrenergic activity of carbuterol (sk&f 40383-a).carbuterol is a beta-adrenergic bronchodilator with selectivity for bronchial smooth muscle relative to cardiac and vascular tissues of several species including man. the present studies were undertaken to further characterize its adrenergic profile. in vitro studies demonstrated that carbuterol was a direct acting beta-adrenergic agonist, not dependent on endogenous catecholamine release, and was devoid of alpha-adrenergic agonist activity. the activity of the racemate was shown to reside prima ...197722441
oxatomide, a new orally active drug which inhibits both the release and the effects of allergic mediators.oxatomide is a new potent inhibitor of anaphylactic and allergic reactions. after oral administration, the compound both inhibits the release of endogenous histamine and prevents the effects of exogensous histamine, at comparable doses. the combination of these effects appears to be the basis of the effectiveness of oxatomide in allergic reactions and may lead to clinical application different from classical antihistaminics and from cromoglycate.197722455
peripheral cardiovascular dopamine receptors. 197722476
changes in the microflora and physiology of the anterior intestinal tract of pigs weaned at 2 days, with special reference to the pathogenesis of diarrhea.the gastrointestinal microflora and gastric physiology of piglets weaned at 2 days was compared with that of piglets allowed to continue sucking the sow. although there was a significantly higher count of escherichia coli in the stomach, duodenum, and jejunum of the early-weaned compared with sow-reared pigs, these differences were not detectable in samples from the ileum. there were no quantitative differences in lactobacilli and in streptococci between the two treatments. lactobacillus ferment ...197722488
association-dissociation of the flavoprotein hog kidney d-amino acid oxidase. determination of the monomer-dimer equilibrium constant and the energetics of subunit association.the enzyme concentration dependence of spectrophotometric titrations of hog kidney d-amino acid oxidase ec 1.4.3.3 with p-aminobenzoate was studied. the monomer-dimer equilibrium constant of the oxidized holoenzyme at 25 degrees c was estimated to be 7 x 10(5)m-1 at ph 7.5 and 4x 10(6)m-1 at ph 8.3. the energetics of subunit association are discussed.197722539
the mechanism of the release of prostaglandin-like activity from guinea-pig isolated ileum. 197722612
an analysis of the inhibitory effects and of possible prostaglandins antagonism of chloroquine in the guinea-pig isolated ileum. 197722625
antidotal action of the oxime hs6 at the soman poisoned neuromuscular junction of the rat and guinea-pig. 197722626
bupropion hydrochloride ((+/-) alpha-t-butylamino-3-chloropropiophenone hcl): a novel antidepressant agent. 197722628
receptors involved in inhibition of the response to acetylcholine released from the myenteric plexus of the guinea-pig ileum proceedings. 197722656
the selective positive inotropic activity of 2-alkyl-1,3-dioxo-5,10-dihydroimidazo1,5-bisoquinolines proceedings. 197722713
changes in muscarinic ligand binding to intestinal muscle strips produced by pre-exposure to hypotonic conditions.the extent of the binding of 3hpropylbenzilylcholine mustard (3h-prbcm) to muscarinic receptors in longitudinal muscle strips from guinea-pig small intestine is increased by nearly 50% when the strips are preexposed to distilled water before measurement of 3h-prbcm binding in krebs-henseleit solution. the apparent rate constant for 3h-prbcm-receptor complex formation is more than double that of intact strips. the curves for the inhibition of 3h-prbcm binding by methylatropinium bromide in normal ...197822721
interaction of mucins with calcium, h+ ion and albumin. 197622809
regulation of energy metabolism in brown adipose tissue mitochondria (author's transl. 197722849
hormones and behaviour. 197722868
properties of membrane-bound and soluble guanylate cyclase of cardiac and skeletal muscle.kinetic properties of guanylate cyclase present in the washed particles, plasma membranes, and the soluble cytoplasm of heart and skeletal muscle are described; properties of the enzyme solubilized by triton x-100 treatment of the particles or membrane fractions are also reported. it is apparent from the data that the membrane-bound guanylate cyclase in the cell may be regulated by acetylcholine, may exist as a metallo-protein with bound mn2+ (essential for activity), and that mg2+ regulates, wh ...197622902
experimental liver failure. a comparison between hepatectomy and hepatic devascularization in the pig.difficulties in the treatment of acute hepatic failure due to insufficient knowledge of the pathogenesis and the impredectability of the course has made an experimental model desirable. a number of such models have been proposed, among these hepatectomy and total hepatic devascularization. in the present study hepatectomy and total hepatic devascularization have been compared, as it has been doubted if hepatectomy, not including necrotic liver tissue, is a valid model of human hepatic failure. n ...197722974
the first cleavage site in pepsinogen activation.by incubation of porcine, bovine, canine, or chicken pepsinogens and calf prochymosin with pepstatin at ph 2.5, the first active protein generated on activation is trapped in an inactive complex. the first activation peptide liberated from porcine pepsinogen has been identified as residues 1-16 whereas that from prochymosin is derived from residues 1-27. this suggests that pepsin and chymosin are not formed by one-step conversions from their zymogens, but by (different) sequential, activation me ...197722994
intramolecular activation of pepsinogen.two pathways for pepsinogen activation have been demonstrated. intramolecular activation, which is kinetically first-order, predominates over the autocatalytic pathway if the ph is below 3 and the protein concentration is less than 1 mg/ml during activation. intramolecular pepsinogen activation is inhibited either by pepstatin, a potent pepsin inhibitor, or by purified globin from hemoglobin, a good pepsin substrate. also, pepsinogen at ph 2 can be bound to a pepstatin-sepharose column and recov ...197722999
metabolic and pharmacologic interactions on the bronchial tonus.after reminding data concerning the part played by the as a rule antagonist camp system in moticity of the smooth muscle fibers in general and of the bronchial fibers in particular, the authors study the different types of interaction on bronchial muscular fibers which have been discribed. it is concerning the adrenergic fibers that the experimental and clinical data are prevalent (role of bronchial alpha- and beta2- receptors) and the authors point especially to their own studies performed sinc ...197723051
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