| effects of "natural oestrogen" replacement therapy on menopausal symptoms and blood clotting. | in a double-blind study on the value of equine ("natural") oestrogens 30 patients presenting with menopausal symptoms in a group practice were monitored for possible adverse effects on blood clotting, weight, and blood pressure. the women were randomly allocated to two groups and given either three months' hormone treatment followed by three months' placebo or vice versa. an appreciable amelioration of all symptoms on placebo made it difficult to asses the genuine value of oestrogen treatment du ... | 1975 | 172181 |
| interaction of cholera enterotoxin with cultured adrenal tumor cells. | in the adrenal tumor cell system ganglioside gm1 inhibited cholera enterotoxin (ct)-induced steroidogenesis if it was preincubated with the toxin or added to adrenal cells 10 min before ct. in the preincubation studies a molar ratio of gm1 to toxin of 3:1 was necessary for half-maximal inhibition of steroidogenesis. on the other hand, horse serum anticholeragenoid neutralized the steroidogenic response to cell-bound ct by 50% if it was added to adrenal monolayer cultures 15 min after the toxin. ... | 1975 | 172445 |
| monocyte activation in horses persistently infected with equine infectious anemia virus. | the monocytes of horses infected with equine infectious anemia virus were shown by their failure to migrate from capillary tubes and their increased adherence to erythrocytes to be activated. | 1975 | 172455 |
| studies on the multiplication of the equine arteritis virus using the fluorescent antibody technique. | | 1975 | 172683 |
| studies on equine viral arteritis. i. characterization of the virus and trial survey on antibody with vero cell cultures. | | 1975 | 172689 |
| the genome of equine arteritis virus. | | 1975 | 173077 |
| activation of influenza a viruses by trypsin treatment. | | 1975 | 173078 |
| equine herpesvirus-induced alterations in nuclear rna and dna polymerase activities. | | 1975 | 173079 |
| transcription of equine herpesvirus type 1: evidence for classes of transcripts differing in abundance. | | 1975 | 173082 |
| prevalence of antibodies to arboviruses in egypt. results of a serologic survey among 1,113 university students. | | 1975 | 173201 |
| equine serum lipids: serum lipoprotein profiles of morgan and thoroughbred horses. | the serum of lipoproteins of 10 morgan and 8 thoroughbred horses were examined by 2 methods of polyacrylamide gel electrophoresis. a significant breed difference in the beta-lipoprotein to alpha-lipoprotein ratio was seen in gradient slab electrophoresis. a breed difference in the number of peaks, but no difference in beta-lipoprotein to alpha-lipoprotein ratio, was found in disc gel electrophoresis. these results have been correlated to indicate differences in charge of alpha-lipoprotein compon ... | 1975 | 173210 |
| equine serum lipids: lipid composition and electrophoretic mobility of equine serum lipoprotein fractions. | the serum lipoprotein fractions from 5 morgan and 5 thoroughbred horses were isolated by preparative ultracentrifugation, chemically analyzed for lipid composition, and studied by 2 methods of polyacrylamide gel electrophoresis to determine electrophoretic mobility. breed differences were not seen in the relative percentages of the lipid classes found in the various fractions. normally, horses, like most animals, carry the majority of their lipid in high-density lipoproteins. electrophoretically ... | 1975 | 173211 |
| investigation of equine infectious anaemia in queensland using gel diffusion. | an antigen for the gel diffusion test for equine infectious anaemia (eia) was prepared from the spleen of a horse experimentally infected with the cq strain of the virus. the antigen produced a single, distinct line of precipitation when tested against a range of known positive serums, and did not react with pre-inoculation and known negative serums. extracts prepared from uninfected spleens displayed no reaction when similarly tested. serum from 34 of 451 queensland horses contained detectable ... | 1975 | 173275 |
| horse liver alcohol dehydrogenase. a study of the essential lysine residue. | 1. the inactivation of horse liver alcohol dehydrogenase by pyridoxal 5'-phosphate in phosphate buffer, ph8, at 10 degrees c was investigated. activity declines to a minimum value determined by the pyridoxal 5'-phosphate concentration. the maximum inactivation in a single treatment is 75%. this limit appears to be set by the ratio of the first-order rate constants for interconversion of inactive covalently modified enzyme and a readily dissociable non-covalent enzyme-modifier complex. 2. reactiv ... | 1975 | 173294 |
| the involvement of cytochrome p-450 in hepatic microsomal steroid hydroxylation reactions supported by sodium periodate, sodium chlorite, and organic hydroperoxides. | the mechanism of steroid hydroxylation in rat liver microsomes has been investigated by employing naio4, naclo2, and various organic hydroperoxides as hydroxylating agents and comparing the reaction rates and steroid products formed with those of the nadph-dependent reaction. androstenedione, testosterone, progesterone, and 17beta-estradiol were found to act as good substrates. naio4 was by far the most effective hydroxylating agent followed by cumene hydroperoxide, nadph, naclo2, pregnenolone 1 ... | 1976 | 173555 |
| biosynthesis and secretion of testosterone by adrenal tissue from the north american opossum, didelphis virginiana, and the effects of tropic hormone stimulation. | | 1975 | 173619 |
| carbohydrate oxidation and antibody function in equine anti-diphtheria immunoglobulin t. | | 1975 | 173648 |
| coital exanthema in stallions. | equine coital exanthema can be produced experimentally in stallions by inoculation with an equine herpesvirus (strain 65/61) and be transmitted during coitus with an infected mare. serological responses to this infection include the production of complement-fixing and serum-neutralizing antibodies which reach maximum levels 14 to 21 days after infection. complement-fixing antibodies decline rapidly and are usually not detectable by 60 days after infection, whereas serum-neutralizing antibody act ... | 1975 | 173847 |
| experimental studies on equine herpesvirus type 1 infections. | the ehv-1 viruses of fetal origin grew better and had a wider tissue culture host range than those isolated from horses with respiratory diseases. comparisons of a fetal isolate (f/304) and a respiratory disease isolate (r/nm-3) in partly immune horses showed that the f/304 virus infected horses more readily, grew better in the nasopharynx, was more likely to cause abortion, and was excreted to a greater extent into the environment. | 1975 | 173849 |
| primary structure determination of two cytochromes c2: close similarity to functionally unrelated mitochondrial cytochrome c. | the amino-acid sequences of the cytochromes c2 from the photosynthetic non-sulfur purple bacteria rhodomicrobium vannielii and rhodopseudomonas viridis have been determined. only a single residue deletion (at position 11 in horse cytochrome c) is necessary to align the sequences with those of mitochondrial cytochromes c. the overall sequence similarity between these cytochromes c2 and mitochondrial cytochromes c is closer than that between mitochondrial cytochromes c and the other cytochromes c ... | 1976 | 174109 |
| spontaneous tumours in young animals. | | 1975 | 174124 |
| [latest results in the research of infectious anemia in perissodactyla]. | | 1975 | 174243 |
| equine infectious anemia. | | 1975 | 174411 |
| [purification and study of equine infectious anemia virus antigens, produced in vivo or in cultured cells]. | the g and c antigens of equine infectious anemia virus have been produced in vivo and in infected cell culture, then they have been purified. their physico-chemical properties have been determined, particularly their molecular weight, their sedimentation coefficient, their density and the iso electric point. | 1975 | 174831 |
| actions of pregnant mare serum gonadotropin in the immature female rat: correlative changes in blood steroids, gonadotropins, and cytoplasmic estradiol receptors of the anterior pituitary and hypothalamus. | several blood steroids, serum gonadotropins and cytosol estradiol receptors of the anterior pituitary and hypothalamus were quantified in immature female rats which were induced to ovulate with pregnant mare's serum gonadotropin (pmsg). studies revealed that serum levels of progesterone, 17-hydroxyprogesterone, testosterone, androstenedione and estradiol were initially elevated at 6 pm (day 30) after administration of 8 iu of pmsg at 10 am day 30. serum levels of estradiol and testosterone rose ... | 1976 | 174887 |
| interaction between dehydrogenases and a new nad -isomer. | a new nad -isomer was prepared, in which the d-ribose of the adenosine moiety was substituted by the enantiomeric l-ribose. as compared to nicotinamide-adenine-dinucleotide (nad) and nadh the coenzyme isomer (d,l)-nad and its dihydroform (d,l)-nadh are far less tightly bound to lactate dehydrogenase and alcohol dehydrogenase from horse liver. in the presence of the second substrate (d,l)-nad and (d,l)-nadh act as hydrogen acceptor and hydrogen donator, respectively, with lactate dehydrogenase an ... | 1975 | 175598 |
| serologic examination of birds from the area of southern moravia for the presence of antibodies against arboviruses of the groups alfa, flavo, bunyamwera supergroup, and the virus yaba 1-lednice 110. i. domestic fowls. | 196 geese, 141 ducks, and 100 hens from 9 localities of the south moravian region (district of breclav) were examined by means of the haemagglutination-inhibition test (hit) for the presence of antibodies against 12 arbovirus antigens of the groups alfavirus (western equine encephalitis (wee), eastern equine encephalitis (eee), semliki, sindbis, chikungunya, o'nyong-nyong), flavovirus (tick-borne encephalitis (tbe), dengue and west nile virus (wn), bunyamwera supergroup (tahna and beta calovo), ... | 1975 | 175616 |
| evidence of respiratory tract infection induced by equine herpesvirus, type 2, in the horse. | five horses were experimentally exposed to equine herpesvirus 2 strain lk. two young foals developed chronic pharyngitis (98 and 232 days, respectively). growth characteristics, cytopathic effects (cpe), inclusion body formation, ether sensitivity, and immunofluorescent analysis indicated that the virus recovered from infected animals was a herpesvirus serologically identical with, or at least antigenically related to ehv-2 strain lk. no significant complement-fixing (cf) or virus-neutralizing ( ... | 1975 | 175904 |
| kinetic studies of the oxidation and reduction of chromatium high potential iron-sulfur protein (hipip) by inorganic complexes. comparison of the electron transfer reactivities of hipip and horse heart cytochrome c. | | 1976 | 176192 |
| mode of action of vibrio cholerae enterotoxin in cultured adrenal tumor cells. | the effects of vibrio cholerae enterotoxin on steroidogenesis and on formation of adenosine 3':5'-cyclic phosphate (cyclic amp) in two adrenal tumor cell lines were compared. steroidogenesis was half-maximal at concentrations of 1 ng of cholera toxin/ml in the mutant os-3 cells and 3 ng of cholera toxin/ml in the parent y-1 cells. at the end of an 8-hr incubation, toxin-induced formation of cyclic amp in the mutant cell line was reduced by 90%. a molar ratio of gm1 ganglioside (galactosyl-n-acet ... | 1976 | 176280 |
| immunofluorescent studies on the multiplication of equine arteritis virus in vero and e. derm (nbl-6) cells. | | 1975 | 176497 |
| [antiviral effect of acridine derivative c 283]. | | 1976 | 176540 |
| adenoviral infection in a foal. | | 1976 | 176763 |
| equine herpesvirus in vivo: cyclic production of a dna density variant with repetitive sequences. | | 1976 | 176793 |
| cell-free synthesis of equine herpesvirus type 3 nucleocapsid polypeptides. | | 1976 | 176794 |
| effects of crude extracts of various plants on infectious bovine rhinotracheitis virus-plaque production. | extracts of 28 plants were tested without demonstable antiviral activity in an agar-overlay plaque-reduction antiviral assay system, using infectious bovine rhinotracheitis virus and bovine endocardial cell cultures. ethanolic extract of narcissus tazetta l bulb elicited antiviral activity by inhibition of viral plaque formation. antiviral activity was demonstrated against infectious bovine rhinotracheitis and equine rhinopneumonitis viruses. narcissus tazetta l bulb did not directly inactivate ... | 1976 | 176907 |
| letter: efficacy of modified live-virus equine rhinopneumonitis vaccine. | | 1976 | 176909 |
| characterization of protein phosphokinase activities in horse thyroid nuclei. | the distribution of protein phosphokinase (ec 2.7.1.37) activities has been established in horse thyroid nuclei. the presence of several enzyme activities has been demonstrated, two of which are clearly distinct. the first one acts on histone as substrate and is activated by cyclic amp. physico-chemical properties of this nuclear cyclic amp-dependent histone kinase and of the cytosol histone kinase are different, demonstrating the absence of a contamination from the cytosol. the second enzyme ac ... | 1976 | 177064 |
| involvement of macrophages in genetic resistance to bone marrow grafts. studies with two specific antimacrophage agents, carrageenan and silica. | carrageenans and silica, agents toxic for macrophages, were used in this study to examine the role of macrophages in resistance of irradiated mice to inbred parental and rat bone marrow grafts. administration of 2.5 mg of carrageenans or 2.5-5 mg of silica particles intravenously to prospective graft recipients resulted in a prompt abrogation of hybrid and xenogeneic resistance. the macrophage stabilizer poly-2-vinylpyridine n-oxide (pvno) injected subcutaneously in the dose of 150 mg/kg, 24 hr ... | 1975 | 177111 |
| low-affinity interaction of ethanol with the fluorescent complex between horse liver alcohol dehydrogenase and auramine o. | quenching of the fluorescence of the complex between horse liver alcohol dehydrogenase (alcohol:nad+ oxidoreductase (ec 1.1.1.1) and auramine o complex is inconsistent with a simple competitive displacement of auramine o by ethanol. instead, the action of ethanol requires an explanation in terms of a solvent effect, or the formation of an enzyme-auramine o-ethanol ternary complex. the latter complex would have to be the low-affinity variety similar to the enzyme-nadh-ethanol ternary complex enco ... | 1976 | 177156 |
| in vitro growth characteristics of virally transformed murine myeloid cells. | bone marrow from normal balb/c mice, mice with myeloid leukemia induced by soule myeloid leukemia virus, and mice with virally induced mammary carcinoma was cultured in semisolid agar. bone marrow from either leukemic or mammary tumor-bearing mice produces more clones in vitro in the presence of a specific colony-stimulating factor. however, in all cases, the myeloid progenitor cells have similar requirements for the colony-stimulating factor. the optimum condition for growth in all instances is ... | 1976 | 177204 |
| serological detection of equid herpesvirus 1 infections of the respiratory tract. | an investigation was made of 3 serological tests (virus neutralization, complement fixation and indirect immunofluorescence), which are applicable to epidemiological studies of infections by equid herpesvirus 1 (ehv-1). sera from gnotobiotic foals inoculated intranasally with various strains of ehv-1 were unable in some cases to neutralize heterologous strains and these results were not consistent with the existence of clearly-defined subtypes of ehv-1, as previously proposed. the cross-reaction ... | 1976 | 177281 |
| horse-liver alcohol dehydrogenase and pseudomonas testosteroni 3(17)beta-hydroxysteroid dehydrogenase transfer epimeric hydrogens from nadh to 17beta-hydroxy-5alpha-androstan-3-one. an exception to one of the alworth-bentley rules. | in the reduction of 17beta-hydroxy-5alpha-androstan-3-one to the 3beta-alcohol, horse liver alcohol dehydrogenase utilizes the 4-pro-r hydrogen of nadh whereas the 3(17)beta-hydroxysteroid dehydrogenase of pseudomonas testosteroni utulized the 4-pro-s hydrogen. these observations provide an exception to the rule proposed by alworth and bentley that with regard to the paired methylene hydrogens at c-4 of nadh and nadph "the stereospecificity of a particular reaction is fixed and does not vary wit ... | 1976 | 177288 |
| hepatomas in grs/a male mice treated with horse serum or horse anti-mouse antilymphocyte serum. | | 1976 | 177290 |
| the effect of tilorone hydrochloride on the growth of several animal viruses in tissue cultures. | tilorone hydrochloride, at a concentration of 10 mug/ml inhibits the growth of herpes simplex virus type 1 in bs-c-1 cells. the growth of vaccinia virus in bs-c-1 cells is partially inhibited; however, six viruses containing rna, including four members of the togavirus group grown in chick fibroblasts, are not affected by the drug. the inhibition of the growth of herpes virus by tilorone hydrochloride is greater when the multiplicity of infection is lower than 1 p.f.u/cell and when the drug is a ... | 1976 | 177724 |
| purification of equine herpesvirus type 1. | a method for the purification of enveloped infectious equine herpesvirus type 1 (ehv-1) is presented. virus from cell culture fluids harvested at 48 h post infection was concentrated by sedimentation and partially purified by differential precipitation with ammonium sulphate. the final steps of purification consisted of two cycles of flotation of virus in pre-formed cscl density gradients. yields of infectious virus were about 30% (18 to 44%) of that present in starting material. as judged by el ... | 1976 | 177732 |
| viral respiratory infections. | | 1976 | 177853 |
| viral antigen production in horse kidney cell cultures infected persistently with equine infectious anemia virus. | | 1976 | 177893 |
| recrudescence of equine infectious anemia by treatment with immunosuppressive drugs. | horses which had passed a few months to a few years asymptomatically after the last recurrence of equine infectious anemia (eia) showed a typical febrile response after treatment with the immunosuppressive agent, dexamethasone (dm) or cyclophosphamide (cy). in horses showing a febrile response, eia virus which had not been neutralized by neutralizing antibody previously produced was propagated. in dm-treated horses it disappeared from the blood soon after pyretolysis and antibody against the vir ... | 1976 | 177894 |
| [equine rhinopneumonitis virus infection in horses. ii (author's transl)]. | | 1976 | 178066 |
| abortion associated with mixed leptospira/equid herpesvirus 1 infection. | | 1976 | 178087 |
| practical aspects of equine virus abortion in the united kingdom. | the current knowledge of the clinical signs, epidemiology and pathogenesis of abortion due to the equid herpesvirus (ehv 1) is reviewed. the relationship between the respiratory and abortigenic forms of the disease is discussed as well as the low incidence of virus abortion in the uk compared to some other parts of the world. some practical aspects of the disease as they affect stud management are considered including methods of diagnosis, prophylaxis and the necessary action to be taken to prev ... | 1976 | 178088 |
| [case of rhinopneumonia in horses in kirghizia]. | | 1976 | 178089 |
| three-dimensional structure of horse liver alcohol dehydrogenase at 2-4 a resolution. | | 1976 | 178875 |
| further studies of specificity of antibodies contained in antiserum against poliovirus. | antigenic components of mahoney strain (poliovirus type 1) involved in virus neutralization reaction were analyzed with mutant mahoney strains resistant to inhibitors in equine serum (inhibitor-resistant mutants) by means of the kinetic neutralization test. it was shown that absorption of anti-mahoney serum with five inhibitor-resistant mutants yielded sera with different antibodies, of which three had distinct specificities and two specificities possibly partly related to one of those three ser ... | 1976 | 178940 |
| effect of sex hormones on protamine inactivated and resistant postheparin plasma lipases. | effects of estrogens and the synthetic anabolic-androgenic steroid, oxandrolone, on extrahepatic (protamine inactivated) and hepatic (protamine resistant) lipases in postheparin plasma, were assessed in 15 subjects with familial hypertriglyceridemia. in six women receiving conjugated equine estrogen (1.25 mg/day), mean (+/- se) postheparin protemine inactivated triglyceride lipase (pi-tgl) was depressed to 0.23 +/- 0.10 mumol ffa/ml/hr, and protamine resistant lipase was depressed to 5.3 +/- 0.5 ... | 1976 | 178980 |
| treatment for azoturia and tying-up. | | 1976 | 178990 |
| [electroencephalographic changes in patients with arbovirus infections]. | | 1976 | 179073 |
| letter: equine virus abortion. | | 1976 | 179184 |
| the response of the immature rat ovary to gonadotrophins: acute changes in cyclic amp, progesteron, testosteron, androstenedione and oestradiol after treatment with pms or fsh + lh. | radioimmunoassays were used to measure changes in progesterone, testosterone, androstenedione, oestradiol, gonadotrophin and ovarian cyclic amp in immature female rats during the first 24 h after exposure to slowly (pms) or rapidly (fsh + lh) disappearing gonadotrophins. cyclic amp was increased 30 min after injection of either kind of gonadotrophin but it had returned to control level within 4 h. serum and ovarian testosterone and androstenedione also increased to a peak at 30 min but decreased ... | 1976 | 179259 |
| immunity to equine herpesvirus type 1 (rhinopneumonitis): in vitro lymphocyte response. | twenty-two ponies were examined for serum-neutralizing (sn) antibody to equine herpesvirus type 1 and for in vitro lymphocyte transformation in the presence of viral antigen. six ponies had undetectable levels of neutralizing antibody (titer less than 1:2) and had lymphocytes which did not respond in culture with viral antigen (stimulation index less than 2.0). four ponies which had sn antibody to equine herpesvirus type 1 did not manifest lymphocyte transformation in vitro. the 12 remaining ser ... | 1976 | 179451 |
| metallocytochromes c: characterization of electronic absorption and emission spectra of sn4+ and zn2+ cytochromes c. | tin (sn4+) and zinc (zn2+) derivatives of horse heart cytochrome c have been prepared and their optical spectra have been characterized. zinc cytochrome c has visible absorption maxima at 549 and 585 nm and soret absorption at 423 nm. tin cytochrome c shows visible absorption maxima at 536 and 574 nm and soret absorption at 410 nm. unlike iron cytochrome c in which the emission spectrum of the porphyrin is almost completely quenched by the central metal, the zinc and tin derivatives of cytochrom ... | 1976 | 179813 |
| n-acetylserine in horse muscle acylphosphatase. | a ninhydrin-negative peptide fraction obtained from tryptic digest of carboxymethyl acylphosphatase was isolated by chromatography on a column of pa 28 beckman resin and analysed for the amino acid composition. degradation with carboxypeptidase b and a indicated that the sequence of this peptide was: x-thr-ala-arg. the amino-terminal residue was identified as n-acetylserine by high voltage electrophoresis. it is therefore suggested that the sequence of the nh2-terminal portion of cm-acylphosphat ... | 1976 | 179962 |
| letter: kinetics of reduction of horse-heart ferricytochrome c by catechol. | | 1976 | 180074 |
| [formation of a complex between carbon monoxide and cytochrome c]. | the conditions of structural modifications of horse heart cytochrome c (ph, salt concentration) have been studied. under these conditions the rate of carboxycytochrome c formation greatly increases in the course of the reduction process as compared to this rate after cytochrome c reduction and relaxation to the equilibrium state. according to these results the reduced intermediate which appears in the course of reduction has a high affinity for the carbon monoxide. it has been shown that the red ... | 1975 | 180400 |
| meningoencephalomyelitis in horses associated with equine herpesvirus 1 infection. | during an outbreak of abortion caused by equine herpesvirus 1, a neurologic disease characterized clinically by dullness and ataxia occurred in several mares. equine herpesvirus 1 was isolated from brain and lung of two severely affected mares. histologically, both mares had disseminated meningoencephalomyelitis characterized by necrotizing arteritis, focal malacia in grey and white matter of brain and spinal cord, and accumulation of lymphocytes and neutrophils in paravertebral ganglia. eosinop ... | 1976 | 180650 |
| disseminated necrotizing myeloencephalitis: a herpes-associated neurological disease of horses. | equine viral rhinopneumonitis type i virus was isolated from spinal cord and brain of a paraparetic horse with disseminated necrotizing myeloencephalitis. necrotic arteriolitis,nonsuppurative necrotizing myeloencephalitis and gasserian ganglioneuritis were present. on record were 12 more cases of horses with similar lesions. the horses had been ataxic or paretic for up to several weeks. a field survey indicated that 14 of 24 horses with acute myelitic signs developed them after recent exposure t ... | 1976 | 180652 |
| [isolation of reoviruses from mink]. | in this first report of the isolation of reovirus from mink, isolates were obtained from 18 to 26 young mink with viral enteritis, by using cultures of cat kidney cells. the isolated also produced cytopathic changes in cell cultures of mink kidney, dog kidney, piglet kidney, calf kidney, bovine embryonic kidney and calf testis. a characteristic feature was the formation of eosinophilic inclusion bodies in the cytoplasma of culture cells. haemagglutination tests were negative with erythrocytes fr ... | 1975 | 180927 |
| isotope effects in the binding of nadh to equine liver alcohol dehydrogenase. | the isotope effect upon the binding constant of nadh to equine liver alcohol dehydrogenase is determined with a method in which the isotopic ratio is measured concurrently in the free and the bond form of the coenzyme, by use of a propellent-pressurized ultrafiltration apparatus for separation of the two. the value for kh/kd for the binding constants was 1.00 +/- 0.02 at ph 10.3 and 25 degrees c. | 1976 | 181045 |
| host preference of mosquito vectors of japanese encephalitis. | the host preference of 4 culex mosquito species collected in miaoli and pingtung counties, taiwan was studied by capillary precipitin method. antisera to alum-precipitated sera of man, bovine, swine, rabbit, horse, dog, cat, mouse, chicken, duck, and pigeon were produced in rabbits and reacted with 758 mosquito blood meals among which reactions to one or more antisera. culex annulus and culex tritaeniorhynchus summorosus showed a great avidity for pig, and culex fuscocephala for bovine. culex pi ... | 1975 | 181218 |
| spectroscopic investigation of binary and ternary coenzyme complexes of yeast alcohol dehydrogenase. | corrected fluorescence properties of yeast alcohol dehydrogenase and its coenzyme complexes have been investigated as a function of temperature. dissociation constants have been obtained for binary and ternary complexes of nad and nadh by following the enhancement of nadh fluorescence or the quenching of the protein fluorescence. it is found that the presence of pyrazole increases the affinity of nad to the enzyme approximately 100-fold. the formation of the ternary enzyme - nad - pyrazole compl ... | 1976 | 181250 |
| do evolutionary changes in cytochrome c structure reflect functional adaptations? | following the demonstration that the rate of evolutionary change in the amino acid sequences of cytochromes c of eukaryotic species was not constant either for a single line of phylogenetic descent during different evolutionary intervals or for separate lines of descent, the concept that neutral mutations account for the vast majority of the evolutionary variations could no longer be accepted. previous studies had shown that all eukaryotic cytochromes c tested appeared to be functionally indisti ... | 1976 | 181272 |
| host immune responses after administration of inactivated venezuelan equine encephalomyelitis virus vaccines. ii. kinetics of neutralizing antibody responses in donors and adoptively immunized recipients. | lymphoid cell responses to immunization with various formalin-inactivated venezuelan equine encephalomyelitis (vee) virus vaccines were monitored in mice by assessment of the development of both the neutralizing antibody response in sera of spleen cell donors and the adoptive neutralizing antibody response induced by spleen cell transfer in recipients. donors immunized intraperitoneally with formalin-inactivated vee vaccine (a single dose or a dose on three consecutive days) developed early seru ... | 1976 | 181497 |
| growth of equine arteritis virus in cells derived from infectious canine hepatitis virus-induced hamster tumor and transformed cells. | | 1976 | 181624 |
| studies on equine viral arteritis. ii. a serological survey of equine viral arteritis in horses imported in 1973/74. | | 1976 | 181627 |
| serological study of an outbreak of paresis due to equid herpesvirus 1 (ehv-1). | six cases of paresis occurred in a swedish stud with 48 mares and a stallion. complement-fixation tests revealed a recent infection with ehv-1 in most horses of the stud. serumneutralisation tests showed rapid antibody-titre increases during the course of the disease. this type of antibody response was interpreted as induced by reinfection or, possibly, recurrent infection. two diseased mares were sacrificed. no virus could be isolated from their central nervous system (cns), liver or spleen, bu ... | 1976 | 181891 |
| equine infectious anaemia. | | 1976 | 181892 |
| regulation of the activities of the enzymes involved in the metabolism of steroid hormones in rat liver: the effect of administration of anterior hypophyseal hormones and gonadotrophin preparations to hypophysectomized rats. | a crude human hypophyseal extract (he), as well as human growth hormone (gh), ovine prolactin (prl) and commercial preparations of acth, tsh, pregnant mare's serum gonadotrophins (pms) and chorionic gonadotrophin (cg) were tested for their ability to induce the activities of cytoplasmic 17 beta-hydroxysteroid dehydrogenase and microsomal delta 4-5alpha-hydrogenase and to repress the activities of microsomal 3alpha- and 3beta-hydroxysteroid dehydrogenases in the liver of hypophysectomized rats. t ... | 1976 | 181936 |
| oocyte maturation and glycolysis in isolated pre-ovulatory follicles of pms-injected immature rats. | graafian follicles were isolated by microdissection from the ovaries of pms-injected immature rats killed at specific times on the day before ovulation. they were incubated in krebs bicarbonate buffer containing 5 mm glucose and 1% bovine serum albumin. the oocytes were recovered after incubation and examined by nomarski interference contrast microscopy. the amount of lactate and glucose in the incubation medium was analysed enzymatically. oocytes recovered from follicles extirpated in the morni ... | 1976 | 181938 |
| catalytic iodination of proteins by horse myeloperoxidase in solid state. | | 1976 | 182036 |
| electron transfer between soluble and immobilized mammalian cytochrome c. equilibrium and kinetic studies on immobilized cytochrome c. | horse heart cytochrome c was covalently bound to sepharose 4b and its redox properties were measured under various experimental conditions. the equilibrium constant for the electron exchange between the oxidized and the reduced form of cytochrome c when one of the two forms was in the semi-solid state and the other one in solution was close to 1. matrix-bound ferrocytochrome c is very stable to autoxidation and is not oxidized by o2 even in the presence of mammalian cytochrome oxidase. oxidation ... | 1976 | 182117 |
| resonance raman studies of a c type algal cytochrome. deuterium shifts and a comparison with mammalian cytochrome c. | a c type cytochrome isolated from synechococcus lividus grown on water and 2h2o media, has been studied by resonance raman spectroscopy. the spectra were taken on the oxidized and reduced protein with excitation within the soret band at 441.6 nm to determine whether individual resonance raman bands of the heme shift upon deuterium substitution and also to provide a comparison with the spectra of horse heart cytochrome c. some of the shifts observed with the deuterated heme c are larger than the ... | 1976 | 182237 |
| encephalitis caused by louping ill virus in a group of horses in ireland. | an outbreak of li infection in a group of free range horses is described. three of 4 horses displayed signs of cns disturbance and 2 of these died after illnesses ranging from 2-12 days duration. in both cases a variable degree of viral polioencephalomyelitis was observed. a virus antigenically indistinguishable from a reference strain of li virus was isolated from the brain and cervical cord of a 3 y.o. draft mare. serum samples obtained from 3 of the horses contained hi, cf, precipitating and ... | 1976 | 182486 |
| efficacy of japanese encephalitis vaccine in horses. | the efficacy of japanese encephalitis vaccine in horses has been described from the effect of mass vaccination on the local prevalence of the disease in horses in each district of hokkaido, japan. | 1976 | 182487 |
| studies on two viral strains isolated from the outbreak of equine influenza in japan. | | 1975 | 183035 |
| studies on a test vaccine for equine influenza virus. i. production of a test vaccine. | | 1975 | 183036 |
| studies on a test vaccine for equine influenza virus. ii. field trials. | | 1975 | 183037 |
| letter: isolation and characterisation of equine herpes virus type 1. | | 1976 | 183339 |
| the structural proteins of equine arteritis virus. | | 1976 | 183352 |
| [etiology and clinical aspects of a viral keratoconjunctivitis in foals]. | | 1976 | 183423 |
| apparent propagation of the equine infectious anemia virus in a mosquito (culex pipiens quinquefasciatus say) ovarian cell line. | a tissue culture of culex pipiens quinquefasciatus say ovarian cells appeared to support the growth of equine infectious anemia (eia) virus. shetland ponies inoculated with 2nd, 7th, 9th, and 11th passages of mediums harvested from infected tissue culture had clinical signs of the disease and became eia positive on 11, 19, 23, and 43 days after inoculation, respectively. | 1976 | 183574 |
| purification and characterization of equine infectious anemia virus. | eia virus was purified from equine fetal kidney cell cultures by peg-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. purified eia virus had a density (20 degrees c) of 1.162 and a sedimentation constant of s20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. the dense core may be at various locations inside t ... | 1976 | 183628 |
| equine herpesviruses. 6. sequential infection of horses with types 2, 3 and 1. | the immunological and virological status of 3 foals in respect of equine herpesviruses (ehv) was established and the foals were sequentially infected with ehv2, ehv3 and ehv1. following experimental infection with ehv2, no clinical signs of disease were observed in any foal. the inoculation of ehv3 into the genital tract resulted in lesions of the mucous membrane and perineal skin that were considered typical of equine coital exanthema. following intransal inoculation of ehv3 extensive ulceratio ... | 1976 | 183644 |
| adenovirus pneumonia in an arabian foal. | | 1976 | 183884 |
| [combined vaccination plan against influenza, equine rhinopneumonitis and tetanus for thoroughbred horses]. | | 1976 | 183944 |
| lithogenic bile after conjugated estrogen. | reference is made to a previous paper by bennion et al. describing t he effects of contraceptive steroids on the lipid composition of gall bladder bile. a similar rise in cholesterol saturation of hepatic bile was observed after giving conjugated equine estrogens (.5 mg/kg) to 7 fa sting patients with indwelling t-tubes. liver function tests were norma l and bile culture was negative. 1 ml of hepatic bile was collected aft er 6, 12, and 24 hours for lipid determinations. the administration o ... | 1976 | 184386 |
| metalloprotein electron transfer reactions: analysis of reactivity of horse heart cytochrome c with inorganic complexes. | the reactions of horse heart cytochrome c with fe(ethylenediaminetetraacetate)2-, co(1,10-phenanthroline)3(3+), ru(nh3)6(2+), and fe(cn)6(3-) have been analyzed within the formalism of the marcus theory of outer-sphere electron transfer, including compensation for electrostatic interactions. calculated protein self-exchange rate constants based on crossreactions are found to vary over three orders of magnitude, decreasing according to fe(cn)6(3-) greater than ru(nh3)6(2+) greater than fe(edta)2- ... | 1976 | 184452 |
| dissociation and rate constants of some human liver alcohol dehydrogenase isoenzymes. | adh from human liver forms binary complexes with nadh, associated with a blue shift of the peak of the fluorescence emission of nadh. the wavelength shift is the same for all isoenzymes but the accompanying intensification of the fluorescence is different. the fluorescence is further increased by the formation of the very tight ternary enzyme-nadh-isobutyramide complexes. these properties are similar to those for the horse liver adh, as well as the molecular weight of e=40 000 per active site of ... | 1976 | 184631 |
| effect of follicle-stimulating hormone on cyclic amp levels in young corpora lutea of the rat. | | 1976 | 185106 |
| the affinity and capacity of the lh receptor of the superluteinized rat ovary in relation to progesterone production. | | 1976 | 185107 |