| interaction of some neuroleptic and antidepressive agents with human coeruloplasmin. | | 1976 | 9093 |
| stimulation of the peripheral chemoreceptors with sodium bicarbonate. | the i.v. administration of sodium bicarbonate was found to cause an increase in arterial ph, followed by an increase in paco2. this caused a large increase in lung ventilation in in pao2. oxygen administration in human subjects, and anatomical denervation of the chemoreceptors in dogs, caused a substantial delay in the ventilatory responses to sodium bicarbonate. it was concluded that the i.v. administration of sodium bicarbonate provides a method of testing the presence of peripheral chemorefle ... | 1976 | 9124 |
| purification and properties of alkaline ribonuclease from human serum. | 1. five alkaline ribonucleases (ec 3.1.4.22) were purified about 140- to 1900-fold from human serum by phosphocellulose and deae-cellulose chromatographies and sephadex g-75 filtration, with a total recovery of 22%. these were designated as rnaases 1-5. 2. optimum activities were observed at ph 8.5-8.7 for rnaases 1-4, and at ph 7.5 for rnaase 5. the molecular weights of these enzymes were estimated by gel filtration as 45 000, 32 000, 20 000, 13 000 and 8500, respectively. 3. these rnaases were ... | 1976 | 9140 |
| mode of orthophosphate uptake and atp labeling by mammalian cells. | incubation of hela cells with 32porthophosphate results in more rapid labeling of the gamma-phosphorus of atp than of the intracellular pool of orthophosphate. the specific radioactivity of atp equals that of extracellular orthophosphate after 2 h of incubation. a similar pattern of labeling is seen with human erythrocytes when incubated at physiological concentrations of orthophosphate (2 mm) and ph 7.4-7.8. at lower ph, 6.8-7.2, the rate of orthophosphate uptake increases and exceeds the rate ... | 1976 | 9142 |
| cyclic nucleotides and platelet aggregation. effect of aggregating agents on the activity of cyclic nucleotide-metabolizing enzymes. | the activities of adenylate and guanylate cyclase and cyclic nucleotide 3':5'-phosphodiesterase were determined during the aggregation of human blood platelets with thrombin, adp, arachidonic acid and epinephrine. the activity of guanylate cyclase is altered to a much larger degree than adenylate cyclase, while cyclic nucleotide phosphodiesterease activity remains unchanged. during the early phases of thrombin-and adp-induced platelet aggregation a marked activation of the guanylate cyclase occu ... | 1976 | 9149 |
| the interaction of radioiodinated thyrotropin with plasma membranes. evidence for high affinity binding sites in the thyroid. | the binding of biologically active 125ithyrotropin to purified plasma membranes prepared from bovine thyroid glands was studied. at 4 degrees c, specific binding reached a maximum after 2 h of incubation and a plateau was maintained for up to 20 h. degradation of 125ithyrotropin was undetectable after 2 h of incubation and was only 10% of the total after 20 h. at ph 6.0, at which binding was maximal, a single class of binding sites, having a dissociation constant of approx. 25 nm, was evident. d ... | 1976 | 9155 |
| a study of the inhalation of pentachlorophenol by rats. part v. a protein binding study of pentachlorophenol. | this study examined the effects on pcp binding to bsa by varying the temperature, ph ionic strength, pcp concentration and bsa concentration. it also compared the albumin binding of pcp to the plasma binding of pcp for the rat and human. | 1976 | 9173 |
| extraction of lhrh in human urine: study of the extraction of labelled synthetic hormone. | a method of extraction of synthetic lhrh is studied in human urines, using porous glass (spherosil) and methanol. in the defined conditions the yield is greeter than 80%. it appears that the method is reproducible. the recovery varies essentially with the quantity of spherosil and the ph of methanal. the use of methanol acidified at ph 3 increases the speed and the importance of labelled lhrh recovery. | 1976 | 9184 |
| applications of the extraction and of the radioimmunoassay of lh-rh in human urine. | the existence of endogenous lh rh like immunoreactivity is shown in human urines after appropriate extraction, bu the radioimmunoassay of lh rh. in normaly cycling and menopausal women the quantities of endogenous hormone found in urines are greater after acid extraction than those found after extraction at ph 7. furthermore, the increase observed by extraction in acidified methanol is directly correlated and proportional to the quantity of hormone assayable by extraction at ph 7. the hypothesis ... | 1976 | 9185 |
| oxidation of lactate by human serum. | the oxidation of lactate by lactate dehydrogenase of human serum is described, and the kinetics of the reaction are examined. it seems that at higher ph values which are optimal for the faster moving isoenzymes of lactate dehydrogenase, slower moving entities show only a part of their activity. in contrast, m type isoenzymes are active at lower ph values, where h type entities are partly inactivated. | 1976 | 9208 |
| isoelectric focusing of spectrin components in hereditary spherocytosis. | 1. by isoelectric focusing in 8 m urea, spectrin purified from normal human erythrocytes was resolved into 12 to 15 peptide bands differing by their isoelectric point. most of them were focused between ph 6.4 and 5.2, one at ph 8.7 and some minor components between ph 7.4 and 6.8. 2. the results were not influenced by the erythrocyte population age. 3. spectrin purified from erythrocytes of five patients with hereditary spherocytosis gave similar isoelectric focusing patterns, with the exception ... | 1976 | 9209 |
| isolation and characterization of isoenzymes of human salivary and pancreatic alpha-amylase. | human salivary and pancreatic alpha-amylase (1,4-glucan 4-glucanohydrolase, ec 3.2.1.1) were separated by electrofocusing. in the first case we obtained six isoenzymes with isoelectric points of ph 5.70, 5.72, 6.23, 6.32, 6.73 and 6.88. human pancreatic alpha-amylase has been separated into eight isoenzymes with isoelectric points of ph 5.72, 5.77, 5.88, 6.05, 6.23, 6.69, 6.72 and 6.95. some of the isoenzymes were shown to be sialoproteins; others representing about 80% of the total activity did ... | 1976 | 9210 |
| lysyl oxidase activity in human normal skins and postburn scars. | lysyl oxidase activity of human normal skins derived from the frontal thighs of 33 subjects showed large variations and the mean value was 11 455 +/- 7 172 (s.d.) cpm/g of wet weight tissue. the age of lesion affected the lysyl oxidase activity in postburn scars. granulation tissues showed a fairly low activity; however, the activity increased sharply within 2--3 months, and reached a significantly higher value than that of normal skin. the high level of activity continued for up to 2--3 years, ... | 1976 | 9213 |
| titration of human placental alkaline phosphatase with radioactive orthophosphate. | human placental alkaline phosphatase incorporates radioactive phosphate specifically and covalently at acid ph. by titration of solutions of the purified enzyme with radioactive orthophosphate, the enzyme was shown to incorporate up to 2 phosphate groups per molecule. no evidence was found to suggest that the two sites had different affinities for phosphate. similar titrations can be used to determine the molarity of solutions of non-placental alkaline phosphatases of unknown purity, if these al ... | 1976 | 9215 |
| ultramicromethod for the determination of human arginase in the presence of urea. | a technique for arginase determination in body fluids in the presence of urea is described. 14carginine is hydrolysed by arginase to 14curea and ornithine. 14curea is separated with paper chromatography and measured in a liquid scintillation counter. the experimental conditions including the ph, substrate concentration, activator, solvent for chromatography, urea inhibition, and arginase in hemolysates, are discussed. | 1976 | 9217 |
| circulating tissue antigens. iii. identification and characterization of antigens of limited and of wide body is distribution in human gall bladder bile. presence in serum of patients with acute hepatitis. | three antigens shared by bile and tissues (bt-1, bt-2 and bt-3) and one shared by bile and saliva (ba) were identified in human gallbladder bile by immunodiffusion. the former were detected in all bile specimens examined, whereas the latter was detected only in half. bt-1 was limited in distribution to kidney, urine and bile; whereas bt-2 and bt-3 were widely distributed mainly in liver, lung and bile. the antigens were not present in biles of other mammals tested, with the exception of ba which ... | 1976 | 9219 |
| the effect of ph on folic acid absorption in man. | 1. pteroylmonoglutamic acid (pteglu) absorption has been measured by using the technique of small-intestinal perfusion with tritiated pteglu in normal subjects and in patients with coeliac disease. 2. at similar intrajejunal ph, patients with untreated coeliac disease have significantly less pteglu than normal subjects and patients with treated coeliac disease. 3. the "resting" ph in the jejunum did not differ markedly between normal subjects and patients with coeliac disease. 4. increasing ph d ... | 1976 | 9222 |
| glutathione reductase from human erythrocytes. catalytic properties and aggregation. | the catalytic properties of glutathione reductase from human erythrocytes have been studied over a range of buffer conditions and substrate concentrations. this study provides optimal conditions for determining the basic kinetic parameters of the enzyme. the catalytic behaviour of glutathione reductase is consistent with spatially separated binding sites for its substrates. in certain assays anomalies were observed which are correlated with an inactivation of the enzyme by nadph. concurrent sedi ... | 1976 | 9277 |
| the role of interchain disulphide bridges in the conformational stability of human immunoglobulin g1 subclass. hydrogen-deuterium exchange studies. | the hydrogen-deuterium exchange data of human immunoglobulin g1 (igg1) are interpreted by assuming fast fluctuations of the protein conformation, through which the peptide groups become exposed to the solvent. the probability of solvent exposure of peptide hydrogens reflects a rather loose conformation for native igg in comparison with other globular proteins. the probability of solvent exposure is greater than 10(-3) for half of the peptide groups, which shows that the conformational transition ... | 1976 | 9279 |
| the cardiovascular effects of etilefrine. | intravenous etilefrine increases the pulse rate, cardiac output, stroke volume, central venous pressure and mean arterial pressure of healthy individuals. peripheral vascular resistance falls during the infusion of 1-8 mg etilefrine but begins to rise at higher dosage. marked falls in pulse rate, cardiac output, stroke volume and peripheral bloodflow, accompanied by rises in mean arterial pressure, occur when etilefrine is infused after administration of intravenous propranolol 2,5 mg. these fin ... | 1975 | 9293 |
| effects of dixyrazine and methaqualone on the sleep pattern in normal man. | whole night eeg and polygraphic recordings were made in ten young, healthy, male volunteers after dixyrazine (12.5 mg, 25 mg, 50 mg), methaqualone (250 mg) and isonox (methaqualone 250 mg + etodroxizine 50 mg). a total of 156 recording nights (36 adaptation nights were not included in the analyses) were scored for different sleep stages according to accepted criteria. the smallest dose of dixyrazine (12.5 mg) had no significant effect upon sleep pattern: the larger doses (25 mg and 50 mg) caused ... | 1975 | 9296 |
| evaluation of in vivo parameters of drug metabolizing enzyme activity in man after administration of clemastine, phenobarbital or placebo. | the 24 h urinary excretion of 6beta-hydroxycortisol and d-glucaric acid, the plasma half lives and total clearances of aminopyrine, and serum gamma-glutamyl-transpeptidase activity have been measured in nineteen healthy male volunteers. the study was done double blind and was conducted as a test of induction of microsomal drug metabolizing enzymes during and after daily doses of 6 mg clemastine, 300 mg phenobarbital or a placebo. the urinary excretion of 6beta-hydroxycortisol and d-glucaric acid ... | 1975 | 9297 |
| pharmacokinetics and relative bioavailability of heptabarbital and heptabarbital sodium after oral administration to man. | a method has been developed for the quantitative determination of heptabarbital 5-(1-cyclohepten-1-yl)-5-ethylbarbituric acid in human plasma after administration of single therapeutic doses of the drug. it involves a single extraction step followed by gas chromatography with alkali flame ionization detection, and the results were linear in the concentration range 0.125 - 5.0 mug/ml plasma. the pharmacokinetics and relative bioavailability of heptabarbital and heptabarbital sodium were studied i ... | 1975 | 9299 |
| the physiological disposition of etilefrine in man. | pharmacokinetic and metabolic studies with 3h-etilefrine were performed to assess the importance of a first-pass effect on the pharmacodynamic action of this sympathomimetic amine. identical amounts of 3h-activity, ca. 80% of the dose, were excreted in the urine after intravenous or oral administration, which indicates complete enteral absorption of the drug. comparison of the areas under the plasma curves of unchanged etilefrine after both routes of administration resulted in a bioavailability ... | 1975 | 9300 |
| anionic activation of human salivary amylase. | in all earlier studies on alpha-amylase, the influence of different ions were studied in phosphate buffer. the present report shows the effect of different ions individually with tris and amino acid. though it has been claimed recently that sodium ion is an activator of alpha-amylase, this study reconfirms that sodium ion does not activate human salivary amylase. | 1976 | 9312 |
| the effect of ion-exchange column chromatography on separation of x and y chromosome-bearing human spermatozoa. | separation of x and y chromosome-bearing spermatozoa has been attempted using ion-exchange column chromatography, with cation- and anion-exchange resins of low, intermediate, and high ionic strength. examination of f-bodies on the y chromosome of treated human sperm and progeny resulting from insemination of treated rabbit spermatozoa indicates that in none of the cases investigated did the treatment cause a separation of x and y chromosome-bearing spermatozoa. the treatment does appear to filte ... | 1976 | 9318 |
| equality in survival of x and y chromosome-bearing human spermatozoa. | human x and y chromosome-bearing spermatozoa survived equally well during washing and resuspension in buffers of ph 5.2 and 8.0 and during incubation in these buffers for 11 hours at 37 degrees c. this suggests that alteration of the ratio of living x- and y-bearing spermatozoa by direct treatment is not an effective method of sex ratio alteration. | 1976 | 9319 |
| accumulation of nicotine in the uterine fluid of the six-day pregnant rabbit. | in 6-day pregnant new zealand white rabbits dosed intravenously with 3h-nicotine, the 3h-activity in the uterine fluid was approximately 5 to 11 times greater than that in the plasma at the corresponding times; 3h-nicotine itself accounted for most of this radioactivity. dichlorodiphenyltrichloroethane (ddt) also accumulated in the uterine luminal fluid of 6-day pregnant rabbits, but to a lesser extent. however, nicotine or ddt accumulation did not occur in similarly treated, nonpregnant rabbits ... | 1976 | 9320 |
| oxygen transport impairment in diabetes. | oxyhemoglobin dissociation curves (odc) from zero to full saturation were developed from tests performed on whole blood from various groups of diabetic and nondiabetic healthy subjects. p50 at in-vivo ph was slightly but significantly lower than normal in ambulatory nonacidotic, uncomplicated juvenile diabetics (26.0 vs. 27.3 mm. hg, p less than 0.001), despite increased red cell 2,3-diphosphoglycerate (2,3-dpg) concentrations in diabetic erythrocytes (15.0 vs. 13.7 mumole/gm. hb, p less than 0. ... | 1976 | 9322 |
| gastric mucosal lesions produced by intravenous infusion of aspirin in cats. | aspirin was given by continuous intravenous infusion to 35 intact cats for 7 days in doses ranging from 25 to 200 mg kg-1 day-1. gastric mucosal lesions occurred in 50 to 70% of the animals in the various dosage groups, including deep ulcers in 20%. all of the ulcers were in antral mucosa near its border with oxyntic mucosa. the incidence of lesions, including ulcers, showed no apparent relation to the dose of aspirin. with all but the highest dose, plasma salicylate levels were within or below ... | 1976 | 9330 |
| biochemical basis of an animal model of depressive illness--a preliminary report--. | biochemical analyses of brain samples of an animal model of depression indicate the state of motionlessness observed in response to a conditioned stimulus was due to an excess in functional activity of serotonin. an excess functional activity of serotonin may be directly responsible for human depressive illness. this conflicting conclusion to the currently popular theories of serotonin deficiency was discussed with reference to the animal and clinical data in the literature which are consistent ... | 1976 | 9342 |
| a kallikrein-specific inhibitor in rat kidney tubules. | a kallikrein inhibitor was found in tubules of the rat kidney and purified by chromatography on sephadex g-100. the molecular weight of the inhibitor, estimated by gel filtration and dodecylsulfate electrophoresis, is about 4700. it inhibits the following kallikreins: porcine submanidbular and pancreatic kallikrein, rat kidney and urine kallikrein, and human urine and plasma kallikrein. an inhibition of bovine trypsin was not observed. | 1976 | 9350 |
| alkaline shift in lumbar and intracranial csf in man after 5 days at high altitude. | in six healthy male volunteers at sea level (pb 747-759 torr), we measured ph and pco2 in cerebrospinal fluid (csf), and in arterial and jugular bulb blood; from these data we estimated pco2 (12) and ph for the intracranial portion of csf. the measurements were repeated after 5 days in a hypobaric chamber (pb 447 torr). both lumbar and intracranial csf were significantly more alkaline at simulated altitude than at sea level. decrease in hco3- in lumbar csf at altitude was similar to that in bloo ... | 1976 | 9373 |
| partial purification and characterization of an endo-alpha-n-acetylgalactosaminidase from the culture of medium of diplococcus pneumoniae. | the culture medium of diplococcus pneumoniae contains enzymic activity that cleaves galbeta1 leads to 3galnac from desialized human erythrocyte membrane glycoprotein. the enzyme was purified 180-fold by ammonium sulfate fractionation, gel filtration through a sephadex g-200 column, and deae a-25 sephadex chromatography. the purified enzyme liberates galbeta1 leads to 3galnac from glycopeptides and glycoproteins with galbeta1 leads to 3galnacalpha1 leads to ser and thr moieties. the optimum ph of ... | 1976 | 9374 |
| stabilization of human serum alkaline phosphatase to histidine-induced heat inactivation by tryptic digestion. | 1. serum alkaline phosphatase ec 3.1.3.1 was strongly inactivated by histidine during incubation at ph 8.0 and 45degrees; however, tryptic digestion of the serum strongly protected the enzyme against inactivation by histidine. in the absence of histidine, however, neither heat inactivation of the phosphatase nor the effect of trypsin ec 3.4.21.4 was observed. factors affecting the alkaline phosphatase inactivation were studied further. 2. the effect of trypsin on the histidine-induced heat inact ... | 1976 | 9376 |
| human adenosine deaminase. distribution and properties. | adenosine deaminase exists in multiple molecular forms in human tissue. one form of the enzyme appears to be "particulate". three forms of the enzyme are soluble and interconvertible with apparent molecular weights of approximately 36,000, 114,000, and 298,000 (designated small, intermediate, and large, respectively). the small form of adenosine deaminase is convertible to the large form only in the presence of a protein, which has an apparent molecular weight of 200,000 and has no adenosine dea ... | 1976 | 9388 |
| tetramer-dimer dissociation in homoglobin and the bohr effect. | the ph dependence of the apparent tetramer to dimer dissociation constant has been determined at 20 degrees for both oxy- and deoxyhemoglobins a and kansas. these measurements were made by three different procedures: gel chromatography, sedimentation velocity, and kinetic methods in either of three buffer systems: 0.05 m cacodylate, tris, or glycine with 1 mm edta and 0.1 m nacl between ph 6.5 and 11. the tetramer-dimer dissociation constant of human oxyhemoglobin a decreases from about 3.2 x 10 ... | 1976 | 9390 |
| properties of a human liver ribonuclease. inhibition by polynucleotides and specificity for phosphodiester bond cleavage to yield purine nucleosides at the 5' termini. | a ribonuclease, purified 2500-fold from human liver, was found to be inactive against synthetic homopolynucleotides, whereas synthetic co-polymers containing adenylic acid were rapidly degraded. the specificity of the rnase is unique in that only purine residues, in a 5:4 ratio of guanylic to adenylic acid, are found at the 5' termini of the degradation products of yeast rna. no specificity was observed at the 3' termini of the fragments. when analyzed by deae-cellulose chromatography, approxima ... | 1976 | 9399 |
| steroid 5alpha-reductase in cultured human fibroblasts. biochemical and genetic evidence for two distinct enzyme activities. | various properties of the steroid 5alpha-reductase have been examined in cell-free extracts of skin and of fibroblasts cultured from genital and nongenital skin from control subjects and from patients with several forms of male pseudohermaphroditism. when 20alpha-hydroxy-4-1,2-3h pregnen-3-one was used as substrate, two 5alpha-reductase activities could be demonstrated in intact skin and cultured fibroblasts. the major activity, previously described for microsomes from human prepuce and extracts ... | 1976 | 9407 |
| metalloproteases of human articular cartilage that digest cartilage proteoglycan at neutral and acid ph. | extracts of human articular cartilage contain proteases capable of degrading the proteoglycan component of cartilage matrix at neutral and acid ph. these enzymes have been partially purified by ion exchange chromotography and characterized by disc electrophoresis, inhibition patterns, and action of proteoglycan. three distinct metalloproteases are described. a neutral protease that digests proteoglycan subunit optimally at ph 7.25 has been purified up to 900-fold. it is strongly inhibited by o-p ... | 1976 | 9425 |
| the particulate superoxide-forming system from human neutrophils. properties of the system and further evidence supporting its participation in the respiratory burst. | studies were performed to characterize the previously reported particulate o2--forming system from human neutrophils. of eight reducing agents examined, including glutathione, ascorbic acid, and intermediates of the glycolytic and hexose monophosphate shunt pathways, only the pyridine nucleotides could serve as electron donors. at 0.1 mm pyridine nucleotide, o2- production was relatively independent of ph. the km for nadh was approximately 0.7 mm regardless of ph, while with nadph the km varied ... | 1976 | 9426 |
| detection of a1a2 and a2aam1 heterozygotes among human a blood group phenotypes. | from the variations of alpha-n-acetylgalactosaminyltransferases activities with the ph, evidence was obtained for the recognition of a1a2 heterozygotes in normal a blood group sera. besides, unusual transferase properties associated with two a2 sera from individuals out of aam1 siblings, lead to the identification of the very infrequent a2aam1 genotypes. these results strongly support the simultaneous coexistence of both a1 and a2 transferases in heterozygotes' sera, and bring some new informati ... | 1976 | 9455 |
| sphingomyelinase activity at ph 7.4 in human brain and a comparison to activity at ph 5.0. | a hitherto undescribed sphingomyelinase (sph'ase 7.4) of human brain has been studied in crude and partially purified (3- to 4- fold) extracts of grey matter, and compared to the known sphingomyelinase with an acid ph optimum (sph'ase 5.0). its specificity for sphingomyelin as substrate is similar to that of sph'ase 5.0, but it differs from sph'ase 5.0 in its ph optimum (7.4 vs 5.0) and in a requirement for mg2+ for optimal activity. other properties of sph'ase 7.4 that distinguish it from sph'a ... | 1976 | 9463 |
| phospholipase a in human brain: a1-type at alkaline ph. | | 1976 | 9479 |
| testicular gamma glutamyl-transpeptidase: an index of sertoli cell function in man. | | 1976 | 9507 |
| perturbational effects of inorganic cations on human erythrocyte membranes. | the perturbational effects of monovalent and divalent cations on human erythrocyte membranes were analyzed by examining their influence on kinetic and structural characteristics of trinitrobenzenesulfonic acid (tnbs) incorporation into the amino groups of protein and phospholipid structural components. the stimulatory effects of monovalent cations on tnbs incorporation, which were size-independent and attributed to nonspecific membrane alterations resulting from ionic strength factors, contraste ... | 1976 | 9513 |
| biological properties of cl. perfringens type a, isolated from human subjects inhabiting a hermetic chamber. | this paper gives the results of quantifying and studying the biological properties of cl. perfringens strains isolated from feces of test subjects kept in an enclosure for 34 days. during the experiment the total number of cl. perfringens increased. it was especially true of the strains with an elevated toxigenic activity. this activity correlated with an increase of the titer of alpha-hemolysines and proteolytic enzymes. | 1976 | 9533 |
| pr man runs anti-hospital-week week. | | 1976 | 9576 |
| measurement of pituitary hormones: clinical applications. 5. prolactin. | a sensitive and specific assay for human prolactin has been developed using human prolactin and antiserum distributed by the united states national pituitary agency. plasma prolactin concentrations in control subjects ranged from 0-20 ng/ml. no sex difference in prolactin concentration was observed. a brisk increase in plasma prolactin levels occurred in normal subjects during the administration of chlorpromazine and thyroid stimulating hormone releasing factor (trh). these stimulatory tests of ... | 1976 | 9604 |
| properties of dna ligase from uninfected and virus-infected hela cells. | hela cells contain a high m.w. form of dna ligase which can be completely converted to a low m.w. form. stokes radius, frictional ratio, sedimentation coefficient, molecular weight, ph dependence, and heat inactivation rate of the two forms have been studied. the major properties of the two forms of dna ligase in hela cells (in particular molecular weights and ph dependence) resemble those of the "dimer" and "monomer" structures described in cultured human cells (pedrali, g., spadari, s., ciarro ... | 1976 | 9621 |
| the properties of beta-adrenoceptor antagonists. | after the clinical studies with pronethalol and propranolol confirmed black's hypothesis that drugs which block beta-adrenoceptors were of value in the treatment of patients with angina pectoris and cardiac arrhythmias, other compounds have been described which block beta-adrenoceptors. detailed pharmacological studies with propranolol and comparison of its properties with those of other beta-adrenoceptor blocking drugs have indicated that these drugs may possess properties in addition to their ... | 1976 | 9628 |
| time course of free and n4-acetylated sulfapyridine concentrations in the plasma and saliva of man after sulfasalazine (salicylazosulfapyridine) administration: preliminary findings. | the time course of free and n4-acetylated sulfapyridine (sp) concentrations in the saliva and whole plasma was determined in a healthy male volunteer after a single 2.0 g oral dose of salicylazosulfapyridine (sasp) as four-500 mg commercial, uncoated tablets. the mean (+/- s.d.) plasma: saliva concentration ratios for free and acetylated sp was 2.04 (+/- 0.24) and 3.12 (+/-0.43), respectively, and were independent of plasma concentration and saliva ph. the elimination half-lives of sp and n4-ace ... | 1976 | 9675 |
| infection rate of arboviruses in dutch recruits returning from surinam. | paired serum samples from approximately 650 dutch recruits residing temporarily in surinam, the first sample being collected on arrival, the second when leaving the country, were examined for haemagglutination-inhibiting antibodies to two types of arbovirus (mucambo and restan) isolated from local mosquitoes, and to two types of arbovirus (oriboca and caraparu) isolated from human blood. in a group of inidviduals in whom mild febrile illness had been reported, antibodies had developed in 3.9 to ... | 1976 | 9715 |
| malabsorption caused by hbsag-positive panareteritis nodosa. | a 36 year old man with panarteriitis nodosa (pan) presented over a certain period of time meinly with gastrointestinal symptoms. he was hbsag positive and a "reactive" hepatitis with histologically demonstrated. arterial microaneurysms and stenoses were detected by angiography in the liver, kidney and small intestine. these findings were confirmed by autopsy. vascular occlusions had caused infarctions of the small intestine as well as necroses and ulcerations of the large bowel. chronic ischemia ... | 1976 | 9747 |
| fatal kidney diseases due to analogesic abuse in switzerland. | as part of an investigation into death by poisoning in switzerland we were able to examine the register of deaths at the swiss federal bureau of statistics. all the medical reports which named analgesic nephropathy as being the basic illness and the main cause of death have been taken into consideration in this work. 331 deaths occurred in the five year period 1967-1971, and were studied as closely as the documents allowed. the earliest cases of death arose in the thirties, the maximum number ho ... | 1976 | 9752 |
| preliminary report: parenteral lorazepam in induced epileptic states in man. | the anticonvulsive effect of lorazepam -- one of the newer diazepines -- has been tested in 10 patients with induced epileptic conditions under continuous polygraphic control. the quantitative analysis of the paroxysmal discharges -- i.e. the evolution of the "paroxysmal index" -- shows that 5 mg iv lorazepam normalize the tracings in 2.3 minutes. parenteral lorazepam inhibits pharmacologically-induced electroencephalographic abnormalities in man. its action is slightly delayed. no effect on car ... | 1976 | 9769 |
| the correlation between salivary peroxidase activity, salivary flow rate, and the oxidation-reduction potentials of human saliva and dental plaque suspensions. | 18 subjects, 9 males and 9 females, were examined regarding salivary oxidation-reduction potential, salivary flow rate, salivary peroxidase activity, oxidation-reduction potential of dental plaque samples, and dental health. both the peroxidase activity, expressed as the salivary lactoperoxidase, and the salivary oxidation-reduction potential increased with increasing salivary flow rate. the variation of these variables was obviously due to changes in salivary flow rate during the day. the remar ... | 1976 | 9773 |
| glycerol transport in human red cells. | the kinetics of 14c-glycerol exchange was studied in human red cells. glycerol appeared to be transported by two mechanisms: (i) by facilitated diffusion with permeability depending on glycerol concentration, and (ii) by an unspecific pathway, presumably representing the diffusion of individual glycerol molecules through the membrane with permeability independent of glycerol concentration. the latter permeability was 8 x 10(-8) cm/s at 20 degrees c, it was independent of ph, and had an activatio ... | 1976 | 9776 |
| guanyl cyclase and cyclic guanosine 3',5'-monosphate-phosphodiesterase in human cerebral tumors. | | 1976 | 9785 |
| morphological, cytological and biological observations on viruses isolated from patients with subacute thyroiditis de quervain. | new data on viruses isolated from patients with subacute thyroiditis de quervain are reported. characteristic morphological, cytological, some physico-chemical and biological features of the isolated viruses are described. a possible role of these viruses in human and animal health disorders is discussed. the isolated viruses remain unclassified so far. | 1976 | 9797 |
| autoantibodies in sera of influenza patients. | sera of the influenza patients and healthy controls were tested for some types of autoantibody (sma, ana, abba, ama). they were detected in 83.8% of the patients' sera and in 16.6% of controls. sma were present in 77.4%, ana in 54.8%, and abba in 16.1% of the patient's sera. ama were not detected. a majority of the sera contained more than one autoantibody type. the possible mechanisms of induction of the autoantibodies in virus infection and their possible role in disease are briefly discussed. | 1976 | 9800 |
| experimental infection of macaca mulatta monkeys with lednice (yaba 1) virus. | macaca mulatta monkeys were inoculated with lednice (yaba 1) virus strain 6118. the animals developed no clinical signs and the body temperature and values of complete haematological examination remained within normal limits. in spite of positive immunofluorescence in regional lymph nodes, no virus could be recovered from them by passages in mice. viraemia was not demonstrated. the antibody response was directly proportional to the amount of virus inoculated, i.e. it was significant after a high ... | 1976 | 9803 |
| infection of hela cells with a virulent and an attenuated strain of pseudorabies virus studied by electron microscopy. | hollow-core particles, forming crystals in nuclei, prevailed in hela cells infected with an attenuated strain of pseudorabies virus (prv). after infection with a virulent prv strain, the cells contained mainly fully infectious dense-core particles. these findings might explain the lower susceptibility of hela and some other human cells to infection with attenuated strains of prv as compared to virulent strains. | 1976 | 9806 |
| replication dynamics of tick-borne encephalitis virus in, and glycolytic activity of, human diploid cells. | tick-borne encephalitis (tbe) virus (western subtype) strains replicated very rapidly in human diploid embryonic lung (hdel) cells, namely within the first 24 hours of infection. greater amounts of both intra- and extracellular virus were produced after inoculation of a virulent than of an attenuated strain. with the virulent strain, more infectious virus was found in the medium than in the cells: the opposite was true for the attenuated strain. no cytopathic effect was observed during the perio ... | 1976 | 9821 |
| continuous measurement, using 2 isotopes, of extravascular pulmonary water. application of a new method or the analysis of supply and pulmonary capillary filtration in normal man. | measurement of pulmonary interstitial fluid may be useful in clinical medicine for early diagnosis or measurement of interstitial and/or alveolar pulmonary edema. a modification of the double dilution method of chinard and goresky is proposed. compared to previous methods, this technique, using two isotopes gamma emitters, is rapid, simple, reproductible and may be repeated. the values obtained in normal subjects at rest 3.5 +/- 0.5 ml/kg, are comparable with those of other authors. they increa ... | 1975 | 9859 |
| regional distribution of pulmonary perfusion during fluid overload in man. | the effects of a fluid vascular overload (dextran 40,000, 10 p. 100, 44 +/- ml/kg/45 min) were studied in 9 normal subjects in the upright position. the following were found: an increase in pulmonary blood volume (+30 p. 100), and in extravascular pulmonary water (+ 70 p. 100), a significant fall in pulmonary compliance and an increase in bronchial resistance. radio-isotopic study of the regional distribution of pulmonary perfusion showed a clear inversion with decrease in perfusion at the bases ... | 1975 | 9861 |
| present statues of lung transplantation (author's transl). | it is difficult to sum up the justifications for pulmonary transplantation in man as out of 34 cases carried out up to now only the patient derom, has survived 10 months. in fact, analysis shows that all of the other cases cannot be taken into consideration, as there were technical problems, or the receivers were nearly in the threws of death and this was the case of the patient salvator. two important problems remain to be resolved: first of all that of obtaining grafts in good condition. in fa ... | 1975 | 9868 |
| pulmonary edema due to shock, fat embolism, disseminated intravascular coagulation and post extracorporeal circulation. | the appearance of lesional edema in the region of the alveolar wall constitues the initial phenomenon of involvement of the pulmonary parenchyma during shock lung, fat embolism, dic and post perfusion lung syndrome. the alveolar septum reacts in a monomorphous manner to these various agressions, although the responsible physiopathological factors are numerous and varied. the lung in e.c.c. represents a vertiable experimental post-agressive lung. it has been studied in man from the clinical, radi ... | 1975 | 9870 |
| acute neurogenic pulmonary edema. | neurogenic edema, in the strict sense of the term, has at the present time practically not benefitted from precise hemodynamic investigations in human clinical practice, and owing to this fact, authors still classify them under the heading "mixed edema or of unknown pathogenesis". in contrast with this lack of information in man, animal experimental works are surprising by their coherence and the experimental facility of producing neurogenic edema (cranial hypertension by a small inflatable ball ... | 1975 | 9872 |
| nonhemodynamic pulmonary edema due to toxins. | lesional pulmonary edema caused by substances which are directly caustic for the gastrointestinal pathways (strong acids and bases, inhaled vomit) are opposed by their immediate and long-term gravity with those due to cardiotropic medicamentatous toxins or volemic overloading which lead to curable pulmonary edema. material and human factors, in favour of accidental intoxication are compensated for by the fact that many toxins produce vapours which are strongly irritant for the upper respiratory ... | 1975 | 9874 |
| the effect of antibody on human prostatic acid phosphatase. substrate utilization by enzyme or enzyme-antibody complex. | | 1976 | 9903 |
| a sensitive, precise, and convenient method for determination of 1,25-dihydroxyvitamin d in human plasma. | | 1976 | 9906 |
| extracorporeal membrane oxygenation during bronchopulmonary lavage. | extracorporeal membrane oxygenation (ecmo) in a venoarterial perfusion circuit was used to provide support of gas exchange during bronchopulmonary lavage in a 32-year-old man with pulmonary alveolar proteinosis and severe arterial hypoxemia. prior to the lavage, pao2 during mechanical ventilation with 100% oxygen and positive end-expiratory pressure was only 125 mm hg. extracorporeal perfusion at a flow rate of 3 liters/min, with oxygen delivery of 244 ml/min, increased the pao2 to 227 mmhg and ... | 1976 | 9921 |
| on the toxicology of carbromal. i. estimation of carbromal and its hypnotically active metabolites in rats and humans (author's transl). | to analyze the toxic effects of carbromal it was necessary to have information on the concentrations of carbromal and of its metabolites in the organism. this information can be obtained by a simple method based on gaschromatography that allows rapid, specific, sensitive and quantitative estimation of carbromal and of its hypnotically active metabolites bromoethylbutyramide and ethylbutyrylurea. employing different detectors (flame ionisation or electron capture detector) the limit of detection ... | 1976 | 9922 |
| late-onset acid maltase deficiency. detection of patients and heterozygotes by urinary enzyme assay. | daily urinary excretion of acid maltase (12.78 +/- 2.10 units/24 hr/mg of creatinine, in 11 normal adults) was significantly decreased in ten patients with late-onset acid maltase deficiency (1.33 +/- 0.16 units/24 hr; p less than .001) and 11 heterozygotes (3.27 +/- 0.62 units/24 hr; p less than .001). maximal inhibition of urinary acid maltase activity by antibodies against human placental enzyme was 53% in controls, 30% in heterozygotes, and virtually absent in patients. investigation of ph c ... | 1976 | 9923 |
| studies on the biological halflife of ionized bromine and on its distribution in the human body. | | 1976 | 9932 |
| interaction of diftalone and some of its metabolites with human serum proteins. | | 1975 | 9942 |
| isoprenaline antagonism of cardioselective beta-adrenergic receptor blocking agents on human and rat adipocytes. | 1. the beta-adrenergic blocking potencies of practolol, ici 66082, tolamolol, acebutolol, h 93/26, h 87/07, pindolol and ro 3-4787 were compared with that of propranolol, on human and rat adipocytes. 2. a good correlation was found between the potencies on adipocytes of the two species but not between our results and literature data on antagonism of isopernaline tachycardia in the anaesthetized cat. 3. the results indicate that differences between adrenergic beta-receptors in heart and adipose t ... | 1975 | 9950 |
| a test which may demonstrate in man, intrinsic sympathomimetic activity of beta-adrenoceptor blocking drugs proceedings. | | 1975 | 9951 |
| pharmacological evaluation of cimetidine, a new histamine h2-receptor antagonist, in healthy man. | cimetidine, a new h2-receptor antagonist, was safely administered to eighteen healthy man by the intravenous, intraduodenal or oral route. 2 when gastric secretion was maximally stimulated by either histamine or pentagastrin, the simultaneous administration of cimetidine produced marked inhibition of both acid and pepsin secretion. 3 cimetidine was well absorbed by mouth and had a blood half-life of 2 hours. 4 cimetidine was rapidly excreted via the kidneys and about 70% of the excreted material ... | 1975 | 9952 |
| effect of n-desmethyldiazepam (nordiazepam) and a precursor, potassium clorazepate, on sleep in man. | 1 the effect of n-desmethyldiazepam (nordiazepam, 5 and 10 mg) and potassium clorazepate (15 mg, a precursor of nordiazepam) on sleep was studied in six healthy adult males. electroencephalography (eeg) was used for sleep measures, and analogue scales were used for subjective assessments of well-being and sleep quality. 2 effects on total sleep time were limited to the night of ingestion. there were increases with nordiazepam (5 and 10 mg) (p = 0.05) and 0.001 respectively), and with clorazepate ... | 1976 | 9963 |
| double-ternary complex affinity chromatography: preparation of alcohol dehydrogenases. | a general affinity chromatographic method for alcohol dehydrogenase purification has been developed by employing immobilized 4-substituted pyrazole derivatives that isolate the enzyme through formation of a specific ternary complex. sepharose 4b is activated with 300 mg of cyanogen bromide/ml of packed gel and coupled to 4-3-(n-6-aminocaproyl)aminopropylpyrazole. from crude liver extracts in 50 mm phosphate-0.37 mm nicotinamide adenine dinucleotide, ph 7.5, alcohol dehydrogenase is optimally bou ... | 1976 | 9981 |
| human liver alcohol dehydrogenase: purification, composition, and catalytic features. | alcohol dehydrogenase has been purified from human liver by affinity chromatography. ultracentrifugation, sephadex g-200 chromatography, and amino acid analyses of multiple preparations demonstrate homogeneity of molecular weight. sodium dodecyl sulfate disc gel electrophoresis reveals a single species of molecular weight 42 000. based on a molecular weight of 85 000 for the dimer obtained from the amino acid composition and a molar absorptivity of a280nm0.1% = 0.58, the enzyme contains 3.6-4.2 ... | 1976 | 9982 |
| microheterogeneity of arylsulfatase a from human tissues. | human arylsulfatase a (cerebroside-3-sulfate 3-sulfohydrolase, ec 3.1.6.8) exhibited microheterogeneity on isoelectric focusing in polyacrylamide gels. pure urinary enzyme gave 3 bands of activity with pi values of 4.7, 4.8 and 4.9, whereas purified liver enzyme yielded six equally spaced bands from pi 4.4 to 4.9. detection of enzyme in the gel was made by either methylumbelliferyl sulfate or nitrocatechol sulfate. crude enzyme preparations from human liver, kindey, placenta, brain and testis sh ... | 1976 | 9992 |
| in vitro activation of glycoprotein hormones. hybridization of subunits from thyrotropin, lutropin and human choriogonadotropin. | in vitro assembly of thyrotropin alpha and beta subunits led to an increase in content of alpha helix and beta sheet very similar to that found for gonadotropins. this association-dependent active folding involved the burying of three tyrosine residues tentatively assigned to tyr alpha 41, tyr beta 37 and tyr beta 59 and common to all studied glycoprotein hormones. in vitro hybridizations between alpha and beta subunits of various hormones (thyrotropin, lutropin and choriogonadotropin) from diff ... | 1976 | 9999 |
| a new method for the determination of alpha1-protease inhibitor (alpha1-antitrypsin) phenotypes based on the formation of alpha1-protease inhibitor allele product-elastase complexes. | up until now it has been assumed that the protease-binding property of alpha1-protease inhibitor (alpha1pi) was destroyed by acid starch gel electrophoresis (ph 4.9). analyses on acid starch gel blocks for ph and conductivity changes during and following a typical electrophoretic run showed that it was unlikely that the separating alpha1pi would be exposed to ph values lower than 6.2, and that the allele products, following the passage of the buffer front, were in an environment of constant ph(6 ... | 1976 | 10000 |
| behavioral studies on the enantiomers of butaclamol demonstrating absolute optical specificity for neuroleptic activity. | butaclamol is a member of a new chemical class for which antipsychotic activity in humans has been demonstrated. butaclamol, a racemate, has been resolved into its optical isomers and a separation of activities was found to occur between the (+) and (-) enantiomers. the present experiments show that at doses ranging from 0.1 to 0.3 mg/kg the (+) enantiomer abolished amphetamine-induced (a) stereotyped behavior and (b) rotational behavior in rats with unilateral lesions in the substantia nigra. i ... | 1976 | 10059 |
| establishment and characterization of human neuroblastoma cell lines. | three new tissue culture cell lines, chp-100, chp-126, and chp-134, have been established from explant cultures of human neuroblastoma. the cell lines have been characterized with respect to morphology, chromosomes constitution, growth, neural enzyme content, and their ability to grow in nude mice. the cells grow as dense masses comprised of fibroblast-or neuroblast-like cells with small processes. the cell lines differ in their neural enzyme acitivity. the chromosomal content of the 3 cell line ... | 1976 | 10079 |
| identification of a ribonuclease p-like activity from human kb cells. | an endoribonuclease which cleaves trna precursor molecules has been partially purified from human kb tissue culture cells. this activity is found in cytoplasmic fractions but is not detectable in the nucleoplasm. trna precursor molecules from both e. coli and kb cells are cleaved by this novel activity to produce 5' phosphate-terminated oligonucleotides. e coli rnaase p and the kb cell nuclease both make a single endonucleolytic scission in e. coli trnatyr precursor, thereby separating the 41 ex ... | 1976 | 10085 |
| isolation and identification of benzodiazepine drugs and their metabolites in urine by use of amberlite xad-2 resin and thin-layer chromatography. | we used the method described here to detect and identify seven benzodiazepine derivatives--diazepam, chlorodiazepoxide, nitrazepam, cloxazolam, oxazolam, oxazepam, and medazepam--and their metabolites in the urine of rabbits given the seven drugs orally. we column-chromatographed 25-ml samples of urine on amberlite xad-2. the drugs and their metabolites in the urine were adsorbed by the resin, irrespective of urinary ph, and upon successive elution with methanol and ethyl acetate/methanol/acetic ... | 1976 | 10093 |
| factors contributing to intra-individual variation of serum constituents: physiological day-to-day variation in concentrations of 10 specific proteins in sera of healthy subjects. | using an automated immunoprecipitin method, we assayed human sera for 10 proteins: haptoglobin, orosomucoid, transferrin, alpha1 antitrypsin, alpha2-macroglobulin, igg, iga, igm, complement c3, and complement c4. blood from 14 healthy subjects (25-40y) was sampled on six separate days. from each venipuncture serum was divided into four eliquots; two were assayed on the day of venipuncture and two were frozen and kept until the end of the study, when all of the frozen samples were analyzed in one ... | 1976 | 10094 |
| a search for the best buffer to use in assaying human lactate dehydrogenase with the lactate-to-pyruvate reaction. | highly purified human lactate dehydrogenases i and v were assayed in 17 different buffers, at a variety of reaction ph's. diethanolamine and 2-amino-2-methyl-1,3-propanediol provided the best measurements of the enzyme, assayed lactate-to-pyruvate. however, the commercial preparation of 2-amino-2-methyl-1,3-propanediol contained insoluble matter and was relatively expensive. all of the four buffers nowmost commonly used were found to present difficulties. glycine and pyrophosphate were inhibotor ... | 1976 | 10100 |
| characterization of adenosine deaminase isozymes from normal human erythrocytes. | adenosine deaminase of phenotype ada was partially purified by chromatography on cm-sephadex c-50 and ammonium sulphate precipitation. with deae-sephadex a-50 three isozymes could be detected. a. the km values for the substrate adenosine were found to be 30 mum for each isozyme. b. ph optimum was 7.0 and the molecular weight estimated by gel filtration was found to be 30 000 for each isozyme. c. the heat stability of rbc-ada type 1-1 was greater than type 1-2. the isozyme in type 2-1 representin ... | 1976 | 10102 |
| sialyltransferase in human malignant melanoma. | a glycosyltransferase, cmp-n-acetylneuraminic acid : glycoprotein sialyltransferase was found in human malignant melanoma. activities were measured with desialized glycoprotein as an exogenous acceptor. the enzyme was characterized by means of its ph optimum, 5.5, temperature optimum, 30 degrees c, km values, 10 mum for the sugar nucleotide and 0.3 mm for desialized glycoprotein. it did not require exogenously added metal ions but was slightly stimulated by mg2+. it required detergent for optima ... | 1976 | 10104 |
| serum gamma-glutamyl transpeptidase and physical exercise. | the immediate and delayed influence of exercise of variable type, duration and intensity on the serum gamma-glutamyl transpeptidase activity (gamma-gt) has been examined in athletes and untrained persons. the possible effects of exercise-induced changes in other parameters (plasma free glutamate, serum triglyceride, haemoconcentration) on the measured postexercise serum gamma-gt have been discussed, partly on the basis of our own experimental data. it appears that neither exercise itself, nor an ... | 1976 | 10110 |
| saturable metabolic pathways for ethotoin in man. | 1. the urinary excretion pattern of ethotoin and five metabolites were examined in three patients receiving continuous treatment with ethotoin at two dose levels, in order to investigate the mechanism behind the dose-dependent kinetics of this anticonvulsant drug. 2. the results suggest a partial saturation in the dealkylation process at high dose levels in three patients. 3. a rough approximation of the michaelis-menten constants for different enzymatic processes was attempted. on the basis of ... | 1976 | 10117 |
| the urinary excretion profiles of naltrexone in man, monkey, rabbit, and rat. | a gas-chromatographic method has been developed for the simultaneous determination of naltrexone, alpha-naltrexol, and beta-naltrexol as trimethylsiyl derivatives. analysis of urine from rabbit, monkey, and rat demonstrated that, like man, these species reduce naltrexone primarily to beta-naltrexol. in naltrexone maintenance patients receiving 125 mg po three times per week, an average of 37% of the dose was recovered in 48-hr urine as free naltrexone (0.8%), conjugated naltrexone (7.6%), free b ... | 1976 | 10147 |
| the flicker control chart method. | | 1976 | 10156 |
| serum concentrations of methaqualone after repeated oral doses of a combination formulation to human subjects. | concentrations of methaqualone have been measured in the serum of five male human subjects receiving five consecutive evening doses of a combination formulation containing methaqualone (250 mg), carbromal (300 mg) and benactyzine (0.33 mg) in each tablet. after administration of the first dose, mean peak serum concentrations of methaqualone (1.2 mug/ml) occurred at 3 h. after obtaining peak levels, mean concentrations of methaqualone declined rapidly during the next 6 h and thereafter more slowl ... | 1976 | 10165 |
| lipoperoxidation rates and drug-oxidizing enzyme activities in the liver and placenta of some mammal species during the perinatal period. | lipoperoxidation and drug-metabolizing enzymes were measured in livers and placentas of different mammal species during the perinatal perios. in placentas and fetal livers of rat, rabbit and guinea-pig, cofactor-supported lipoperoxidation was negligible, as were the activities of drug-oxidizing enzymes. human fetal liver contained an intact drug-oxidizing electron transport chain, and lipoperoxidation activity was accordingly abserved. it is suggested that lesions mediated by lipoperoxidation ma ... | 1976 | 10178 |