| chicken-type lysozyme in channel catfish: expression analysis, lysozyme activity, and efficacy as immunostimulant against aeromonas hydrophila infection. | to understand whether chicken-type lysozyme (lys-c) in channel catfish was induced by infection of aeromonas hydrophila, the transcriptional levels of lys-c in skin, gut, liver, spleen, posterior kidney, and blood cells in healthy channel catfish was compared to that in channel catfish infected with a. hydrophila by bath immersion. quantitative pcr revealed that the transcription levels of lys-c in infected catfish were significantly (p < 0.05) induced in all five tissues tested as well as in bl ... | 2013 | 23732847 |
| expression and activity of recombinant proaerolysin derived from aeromonas hydrophila cultured from diseased channel catfish. | aerolysin is one of the putative toxins in extracellular products (ecp) produced by aeromonas hydrophila, an important pathogen of catfish. to better understand the molecular mechanism and mode of action of this toxin, proaerolysin-coding gene was cloned from the genomic dna of an a. hydrophila strain, cultured from diseased channel catfish, and heterologously expressed in e. coli. functional recombinant proaerolysin was obtained, revealing some unique properties. the purified recombinant proaer ... | 2013 | 23680108 |
| establishment and characterization of a fin cell line from blunt snout bream, megalobrama amblycephala. | this study established and characterized a new cell line (maf) from the fin of blunt snout bream (megalobrama amblycephala), a freshwater fish cultivated in china. maf cells proliferated well in medium 199 supplemented with 10 % fetal bovine serum at 28 °c and have been subcultured more than 95 times in almost a year. maf cells were revived at 90-95 % viability after 3-6 months of storage in liquid nitrogen. karyotyping indicated that the modal chromosome number of maf cells was 48. the maf cell ... | 2013 | 23532304 |
| identification, virulence, and mass spectrometry of toxic ecp fractions of west alabama isolates of aeromonas hydrophila obtained from a 2010 disease outbreak. | in west alabama, disease outbreaks in 2009 caused by aeromonas hydrophila have led to an estimated loss of more than $3 million. in 2010, disease outbreak occurred again in west alabama, causing losses of hundreds of thousands of pounds of market size channel catfish. during the 2010 disease outbreak in west alabama, four isolates of a. hydrophila were cultured from the kidney tissues of diseased channel catfish. both analytical profile index (api) 20 e biochemical tests and 16s-23s rrna sequenc ... | 2013 | 23523171 |
| attempt to develop live attenuated bacterial vaccines by selecting resistance to gossypol, proflavine hemisulfate, novobiocin, or ciprofloxacin. | in an attempt to develop attenuated bacteria as potential live vaccines, four chemicals (gossypol, proflavine hemisulfate, novobiocin, and ciprofloxacin) were used to modify the following four genera of bacteria through chemical-resistance strategy: (1) aeromonas hydrophila (9 isolates); (2) edwardsiella tarda (9 isolates); (3) streptococcus iniae (9 isolates); and (4) s. agalactiae (11 isolates). all bacteria used in this study were able to develop high resistance to gossypol. however, only som ... | 2013 | 23499519 |
| characterization of the ceruloplasmin gene and its potential role as an indirect marker for selection to aeromonas hydrophila resistance in rohu, labeo rohita. | ceruloplasmin is an acute phase protein found to be activated by the host immune system during stress conditions. the ceruloplasmin gene has been reported in several teleosts and here we characterize the gene and test its association with resistance to aeromonas hydrophila in rohu, labeo rohita. a ceruloplasmin mrna sequence of 3355 base pairs (bp) was derived (genbank id: jx010736). the coding sequence (cds) comprised of 3276 bp that coded for 1092 amino acids. alignment results showed the grea ... | 2013 | 23481214 |
| evasion of mucosal defenses during aeromonas hydrophila infection of channel catfish (ictalurus punctatus) skin. | the mucosal surfaces of fish serve as the first line of defense against the myriad of aquatic pathogens present in the aquatic environment. the immune repertoire functioning at these interfaces is still poorly understood. the skin, in particular, must process signals from several fronts, sensing and integrating environmental, nutritional, social, and health cues. pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. here, we investiga ... | 2013 | 23219904 |
| identification of bacillus strains for biological control of catfish pathogens. | bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against edwardsiella ictaluri and aeromonas hydrophila, the causative agents of enteric septicemia of catfish (esc) and motile aeromonad septicaemia (mas), respectively. twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including ed ... | 2012 | 23029244 |
| attenuation of a virulent aeromonas hydrophila with novobiocin and pathogenic characterization of the novobiocin-resistant strain. | to determine whether novobiocin resistance strategy could be used to attenuate a virulent aeromonas hydrophila ah11p strain and to characterize the growth and pathogenic differences between the novobiocin-resistant strain and its virulent parent strain ah11p. | 2012 | 22897434 |
| expression profiles of nods in channel catfish (ictalurus punctatus) after infection with edwardsiella tarda, aeromonas hydrophila, streptococcus iniae and channel catfish hemorrhage reovirus. | nlrs are a large family of intracellular pathogen recognition receptors (prrs) that recognize pathogen associated molecular patterns (pamps). in the previous study, the identification of nlrs subfamily a (nods) and gene expression was carried out in channel catfish (ictalurus punctatus). however, the gene expression profiles of channel catfish nods (nod1, nod2, nlrc3, nlrc5 and nlrx1) after infection with various bacteria and virus are still unclear. in this study, expression of five nods genes ... | 2012 | 22796486 |
| rna-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following edwardsiella ictaluri infection in channel catfish, ictalurus punctatus. | the mucosal surfaces of fish (gill, skin, gastrointestinal tract) are important sites of bacterial exposure and host defense mechanisms. in mammalian systems, the intestinal epithelium is well characterized as both a selectively permeable barrier regulated by junctional proteins and as a primary site of infection for a number of enteric pathogens including viruses, bacteria, and parasites. the causative bacterium of enteric septicemia of catfish, edwardsiella ictaluri, is believed to gain entry ... | 2012 | 22366064 |
| multidrug-resistant aeromonas veronii recovered from channel catfish (ictalurus punctatus) in china: prevalence and mechanisms of fluoroquinolone resistance. | to emphasize the importance of the appropriate use of antibiotics in aquaculture systems, the prevalence of resistance to 25 antimicrobials was investigated in 42 aeromonas veronii strains isolated from farm-raised channel catfish in china in 2006-2012. all experiments were based on minimal inhibitory concentrations (mics), and susceptibility was assessed according to the clinical and laboratory standards institute. some isolates displayed antibiotic resistance to the latest-generation fluoroqui ... | 2016 | 27483342 |
| impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, ictalurus punctatus. | one of the highest priority areas for improvement in aquaculture is the development of dietary additives and formulations which provide for complete mucosal health and protection of fish raised in intensive systems. far greater attention has been paid to dietary impact on gut health than to protective effects at other mucosal surfaces such as skin and gill. these exterior surfaces, however, are important primary targets for pathogen attachment and invasion. flavobacterium columnare, the causativ ... | 2015 | 26164837 |
| isolation of bacteroides from fish and human fecal samples for identification of unique molecular markers. | bacteroides molecular markers have been used to identify human fecal contamination in natural waters, but recent work in our laboratory confirmed cross-amplification of several human-specific bacteroides spp. assays with fecal dna from fish. for identification of unique molecular markers, bacteroides from human (n = 4) and fish (n = 7) fecal samples were cultured and their identities were further confirmed using rapid id 32a api strips. the 16s rdna from multiple isolates from each sample was pc ... | 2013 | 24313449 |
| establishment and characterization of a heart-derived cell line from goldfish (carassius auratus). | the goldfish carassius auratus, a freshwater fish in the family cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. a cell line was established from goldfish heart (gh) tissue to create a biological monitoring tool for viral diseases. the gh cell line was optimally maintained at 25 °c in m199 medium supplemented with 10-20% fetal bovine serum. a chromosomal analysis indicated that the cell line remained diploid, with a mean chromosomal count of 100. in viral inoc ... | 2017 | 28097494 |
| austrodiplostomum sp., bolbophorus sp. (digenea: diplostomidae), and clinostomum marginatum (digenea: clinostomidae) metacercariae in inland silverside menidia beryllina from catfish aquaculture ponds, with notes on the infectivity of austrodiplostomum sp. cercariae in channel catfish ictalurus punctatus. | in the southeastern usa, catfish aquaculture is burdened by predation from piscivorous birds and the digenetic trematodes they carry. in addition to cultured ictalurid fish, other forage or incidental fish species inhabit catfish production ponds. of these, the inland silverside menidia beryllina was recently found to harbor larval metacercariae of several trematode species. three species of metacercariae were reported, two of which represent the first morphological descriptions of an austrodipl ... | 2016 | 27539726 |
| validation of fermentation and processing procedures for the commercial-scale production of a live, attenuated edwardsiella ictaluri vaccine for use in channel catfish aquaculture. | mortality associated with edwardsiella ictaluri infection is a serious impediment to the commercial production of fingerling channel catfish ictalurus punctatus. a patented, live, attenuated, orally delivered vaccine has been developed that offers exceptional protection against e. ictaluri infection in both laboratory and small-scale pond trials. further vaccine development is contingent on the successful completion of large-scale field trials that accurately reflect industry conditions. this cu ... | 2017 | 28376313 |
| the nck and abi adaptor genes in catfish and their involvement in esc disease response. | adaptor proteins non-catalytic region of tyrosine kinase (nck) and abelson interactor (abi) are crucial for disease response. nck1 was identified to be a candidate gene for enteric septicemia of catfish (esc) disease resistance, and was speculated to play similar roles during esc and enteropathogenic escherichia coli (epec) pathogenicity. abi1 was reported as a positional candidate gene for bacterial cold water disease (bcwd) resistance in rainbow trout. in this study, three nck genes and six ab ... | 2017 | 28341353 |
| identification and characterization of a β-defensin gene involved in the immune defense response of channel catfish, ictalurus punctatus. | antimicrobial peptides are small peptides that play important roles in a host's innate immune response. as an important antimicrobial peptide, β-defensin widely distribute in mammals, insects and plants with broad-spectrum antimicrobial activity. in this study, the β-defensin gene of the channel catfish, ictalurus punctatus, was cloned, sequenced, and subjected to a bioinformatic analysis. the β-defensin gene of the channel catfish contains three exons and two introns, and encodes a precursor pe ... | 2017 | 28340427 |
| the chemokinome superfamily: ii. the 64 cc chemokines in channel catfish and their involvement in disease and hypoxia responses. | chemokines are a superfamily of structurally related chemotactic cytokines exerting significant roles in regulating cell migration and activation. based on the arrangement of the first four cysteine residues, they are classified into cc, cxc, c and cx3c subfamilies. in this study, a complete set of 64 cc chemokine ligand (ccl) genes was systematically identified, annotated, and characterized from the channel catfish genome. extensive phylogenetic and comparative genomic analyses supported their ... | 2017 | 28322933 |
| gwas analysis of qtl for enteric septicemia of catfish and their involved genes suggest evolutionary conservation of a molecular mechanism of disease resistance. | disease problems cause major economic losses for the aquaculture industries. in catfish, enteric septicemia of catfish (esc), caused by the bacterial pathogen edwardsiella ictaluri, is the leading disease problem, causing tens of millions of dollars of annual economic losses. in this study, we conducted a genome-wide association study to determine quantitative trait loci (qtl) for resistance against esc using an interspecific hybrid system. five hundred fish were used in the analysis and 192 phe ... | 2017 | 27826737 |
| identification, annotation and expression analysis of 29 rho gtpase genes from channel catfish (ictalurus punctatus) after bacterial infections. | the rho family gtpases are a group of small monomeric g proteins, which are molecular switches in signaling pathways. they have been known to regulate a diverse range of cellular processes including actin cytoskeleton rearrangement and microtubule dynamics. in particular, their participations in immune responses are also significant. however, little information of the rho gtpases is available in teleost including channel catfish, an economically important species and one of the best teleost mode ... | 2017 | 27765605 |
| ferric hydroxamate uptake system contributes to edwardsiella ictaluri virulence. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen causing enteric septicemia in fish, particularly in channel catfish. ferric iron is an essential micronutrient for bacterial survival, and some bacterial pathogens use secreted hydroxamate-type siderophores to chelate iron in host tissues. siderophore-iron complexes are taken up by these bacteria via the ferric hydroxamate uptake (fhu) system. in e. ictaluri, the fhu system consists of fhuc, fhud, fhub, and fhua genes. h ... | 2016 | 27666508 |
| involvement of tolq and tolr genes in edwardsiella ictaluri virulence. | edwardsiella ictaluri is a gram-negative intracellular facultative pathogen causing enteric septicemia of channel catfish (esc). the tol system, consisting of four envelope proteins tolq, tolr, tola, and tolb, are required for colicin import and contributes to bacterial virulence in several pathogenic bacteria. however, the tol system and its importance in e. ictaluri virulence have not been investigated. here we present construction and evaluation of the e. ictaluri tolq, tolr and tolqr mutants ... | 2016 | 27622343 |
| analysis of apolipoprotein genes and their involvement in disease response of channel catfish after bacterial infection. | apolipoproteins are protein component of plasma lipoproteins. they exert crucial roles in lipoprotein metabolism and serve as enzyme cofactors, receptor ligands, and lipid transfer carriers in mammals. in teleosts, apolipoproteins are also involved in diverse processes including embryonic and ontogenic development, liver and digestive system organogenesis, and innate immunity. in this study, we identified a set of 19 apolipoprotein genes in channel catfish (ictalurus punctatus). phylogenetic ana ... | 2017 | 27640368 |
| edwardsiella ictaluri infection in pangasius catfish imported from west bengal into the southern caribbean. | in response to a mortality event, seven pangasius catfish (pangasianodon hypophthalmus) were submitted to the university of the west indies, school of veterinary medicine, trinidad and tobago, for diagnostic evaluation. these fish were part of a consignment that arrived from kolkata two weeks earlier. fish presented with perianal haemorrhage and blister-like swellings on the skin which ruptured to leave ulcers. edwardsiella ictaluri was consistently recovered from the brain and skin. repetitive ... | 2016 | 27592704 |
| ompn, outer membrane proteins of edwardsiella ictaluri are potential vaccine candidates for channel catfish (ictalurus punctatus). | outer membrane proteins (omps) are a class of proteins that reside in the outer membrane of gram-negative bacteria. omps act as epitopes and are potential vaccine candidates. outer membrane protein n (ompn) is a component of the outer membrane of edwardsiella ictaluri (e. ictaluri). in a previous study, the ompn1-, ompn2-, ompn3-encoding genes of e. ictaluri were cloned, and here they were expressed in escherichia coli. western blotting showed that these three proteins had molecular weights of ∼ ... | 2016 | 27567702 |
| expression of bcl-2 genes in channel catfish after bacterial infection and hypoxia stress. | bcl-2 proteins are of vital importance in regulation of apoptosis, and are involved in a number of biological processes such as carcinogenesis and immune responses. bcl-2 genes have been well studied in mammals, while they are not well investigated in teleost fish including channel catfish, the major aquaculture species in the united states. in this study, we identified 34 bcl-2 genes from the channel catfish genome, and verified their identities by conducting phylogenetic and syntenic analyses. ... | 2016 | 27353474 |
| influence of lipopolysaccharide outer-core in the intrinsic resistance to antimicrobial peptides and virulence in edwardsiella ictaluri. | the genus edwardsiella consists of bacteria with an intrinsic resistance to cyclic cationic antimicrobial peptides (camps). edwardsiella ictaluri, a pathogen of the catfish (ictalurus punctatus) and the causative agent of a systemic infection, is highly resistant to camps. previously, we determined that the oligo-polysaccharide (o-ps) of the lipopolysaccharide (lps) does not play a role in the e. ictaluri camp resistance and an intact core-lipid a structure is necessary for camps resistance. her ... | 2016 | 26945561 |
| phosphoinositide 3-kinase family in channel catfish and their regulated expression after bacterial infection. | the phosphoinositide-3-kinase (pi3ks) family of lipid kinases is widely conserved from yeast to mammals. in this work, we identified a total of 14 members of the pi3ks from the channel catfish genome and transcriptome and conducted phylogenetic and syntenic analyses of these genes. the expression profiles after infection with edwardsiella ictaluri and flavobacterium columnare were examined to determine the involvement of pi3ks in immune responses after bacterial infection in catfish. the results ... | 2016 | 26772478 |
| septin genes in channel catfish (ictalurus punctatus) and their involvement in disease defense responses. | septins are an evolutionarily conserved family of gtp-binding proteins. they are involved in diverse processes including cytokinesis, apoptosis, infection, neurodegeneration and neoplasia. in this study, through thorough data mining of existed channel catfish genomic resources, we identified a complete set of 15 septin genes. septins were classified into four subgroups according to phylogenetic analysis. extensive comparative genomic analysis, including domain and syntenic analysis, supported th ... | 2016 | 26700173 |
| genome-wide identification of hsp70 genes in channel catfish and their regulated expression after bacterial infection. | heat shock proteins 70/110 (hsp70/110) are a family of conserved ubiquitously expressed heat shock proteins which are produced by cells in response to exposure to stressful conditions. besides the chaperone and housekeeping functions, they are also known to be involved in immune response during infection. in this study, we identified 16 hsp70/110 geness in channel catfish (ictalurus punctatus) through in silico analysis using rna-seq and genome databases. among them 12 members of hsp70 (hspa) fa ... | 2016 | 26693666 |
| comparison of edwardsiella ictaluri isolates from different hosts and geographic origins. | the intraspecific variability of e. ictaluri isolates from different origins was investigated. isolates were recovered from farm-raised catfish (ictalurus punctatus) in mississippi, usa, tilapia (oreochromis niloticus) cultured in the western hemisphere and zebrafish (danio rerio) propagated in florida, usa. these isolates were phenotypically homologous and antimicrobial profiles were largely similar. genetically, isolates possessed differences that could be exploited by repetitive-sequence-medi ... | 2016 | 26661707 |
| expression profile analysis of two cathepsin s in channel catfish (ictalurus punctatus) mucosal tissues following bacterial challenge. | cathepsin s belongs to the papain family of cysteine protease, and is considered to play key roles in immune responses after bacterial challenge. however, despite the recognized importance of cathepsin s in immunity, no studies have systematically characterized cathepsin s in catfish. in this regard, here, we characterized the cathepsin s gene family in channel catfish, and investigated their expression patterns following two different gram-negative bacterial challenge. in the present study, two ... | 2016 | 26626584 |
| identification and mucosal expression analysis of cathepsin b in channel catfish (ictalurus punctatus) following bacterial challenge. | the mucosal surfaces of fish (skin, gill and intestine) constitute the primary line of defense against pathogen invasion. although the importance of fish mucosal surfaces as the first barriers against pathogens cannot be overstated, the knowledge of teleost mucosal immunity are still limited. cathepsin b, a lysosomal cysteine protease, is involved in multiple levels of physiological and biological processes, and playing crucial roles for host immune defense against pathogen infection. in this re ... | 2015 | 26497091 |
| efficacy of florfenicol for control of mortality associated with edwardsiella ictaluri in three species of catfish. | the efficacy of florfenicol for control of mortality associated with edwardsiella icatluri was studied in fingerlings of channel catfish ictalurus puntatus (delta strain), blue catfish i. furcatus (d&b strain), and a hybrid catfish (delta strain channel catfish × d&b strain blue catfish). on day 0, fish were immersion challenged in 65-l aquaria. for each of the three species of catfish, 10 aquaria were randomly assigned to two treatment groups, either treated with florfenicol at 0 mg/kg of body ... | 2015 | 26306332 |
| complement regulatory protein genes in channel catfish and their involvement in disease defense response. | complement system is one of the most important defense systems of innate immunity, which plays a crucial role in disease defense responses in channel catfish. however, inappropriate and excessive complement activation could lead to potential damage to the host cells. therefore the complement system is controlled by a set of complement regulatory proteins to allow normal defensive functions, but prevent hazardous complement activation to host tissues. in this study, we identified nine complement ... | 2015 | 26111998 |
| oral vaccination of channel catfish against enteric septicemia of catfish using a live attenuated edwardsiella ictaluri isolate. | enteric septicemia of catfish (esc), caused by edwardsiella ictaluri, is the most problematic bacterial disease affecting catfish aquaculture in the southeastern united states. efforts to develop an effective esc vaccine have had limited industrial success. in commercial settings, esc vaccines are typically administered by immersion when fry are transferred from the hatchery to rearing ponds. while this approach is a practical method of mass delivery, this strategy administers vaccines to very y ... | 2015 | 26030354 |
| hsp90, hsp60 and shsp families of heat shock protein genes in channel catfish and their expression after bacterial infections. | heat shock proteins (hsps) are a suite of highly conserved proteins whose expressions are generally induced by elevated temperature. however, many hsps play important roles in both innate and adaptive immunity. on the basis of our previous work on hsp40 and hsp70 gene families in channel catfish (ictalurus punctatus), the objective of this study was to characterize hsp90, hsp60, hsp10, and small hsp genes, and to investigate their expression profiles after bacterial infections. a total of 20 hsp ... | 2015 | 25827625 |
| identification and expression analysis of 26 oncogenes of the receptor tyrosine kinase family in channel catfish after bacterial infection and hypoxic stress. | receptor tyrosine kinases (rtks) are high-affinity cell surface receptors for many polypeptide growth factors, cytokines and hormones. rtks are not only key regulators of normal cellular processes, but are also involved in the progression of many types of tumors, and responses to various biotic and abiotic stresses. catfish is a primary aquaculture species in the united states, while its industry is drastically hindered by several major diseases including enteric septicemia of catfish (esc) that ... | 2015 | 25722053 |
| effects of a phytogenic feed additive on growth performance, susceptibility of channel catfish to edwardsiella ictaluri and levels of mannose binding lectin. | a study was conducted to investigate the effect of a phytogenic feed additive (digestarom® p.e.p. mge; containing the essential oils carvacrol, thymol, anethol, and limonene) on growth performance and disease susceptibility to edwardsiella ictaluri. two hundred and fifty juvenile channel catfish, ictalurus punctatus (7.2 ± 0.1 g) were allotted into the following treatments: control (floating diet) and eo (floating diet supplemented with essential oils). the fish were fed their respective diets f ... | 2015 | 25659231 |
| identification, phylogeny and expression analysis of suppressors of cytokine signaling in channel catfish. | the suppressors of cytokine signaling (socs) family genes play important roles in regulating a variety of signal transduction pathways that are involved in immunity, growth and development. because of their importance, they have been extensively studied in mammalian species, but they have not been systematically studied among teleost fish species. in this study, a total of 12 socs genes were characterized to understand the molecular mechanisms of socs function in channel catfish. phylogenetic an ... | 2015 | 25555134 |
| expression of tumor suppressor genes in channel catfish after bacterial infections. | tumor suppressor genes are negative regulators of tumor formation. while their anti-tumor functions have been well studied, they have been found to be also involved in immune responses and innate immunity. in this study, 21 tumor suppressor genes in channel catfish (ictalurus punctatus) were characterized. phylogenetic and syntenic analyses allowed annotation of all 21 catfish tumor suppressor genes. the expression profiles of the 21 catfish tumor suppressor genes were determined using the rna-s ... | 2015 | 25453578 |
| comparative susceptibility of channel catfish, blue catfish, and their hybrid cross to experimental challenge with bolbophorus damnificus (digenea: bolbophoridae) cercariae. | the digenetic trematode bolbophorus damnificus has been implicated in significant losses in catfish aquaculture since the late 1990s. the complex life cycle sequentially involves the american white pelican pelecanus erythrorhynchos, the marsh rams horn snail planorbella trivolvis, and channel catfish ictalurus punctatus. research supports anecdotal reports from the industry, suggesting that the hybrid of channel catfish×blue catfish i. furcatus is less susceptible to disease agents that have bee ... | 2014 | 24895863 |
| tissue persistence and vaccine efficacy of tricarboxylic acid cycle and one-carbon metabolism mutant strains of edwardsiella ictaluri. | edwardsiella ictaluri causes enteric septicemia in fish. recently, we reported construction of e. ictaluri mutants with single and double gene deletions in tricarboxylic acid cycle (tca) and one-carbon (c-1) metabolism. here, we report the tissue persistence, virulence, and vaccine efficacy of tca cycle (eiδsdhc, eiδfrda, and eiδmdh), c-1 metabolism (eiδgcvp and eiδglya), and combination mutants (eiδfrdaδsdhc, eiδgcvpδsdhc, eiδmdhδsdhc, and eiδgcvpδglya) in channel catfish. the tissue persistenc ... | 2014 | 24837777 |
| pathogen recognition receptors in channel catfish: iv. identification, phylogeny and expression analysis of peptidoglycan recognition proteins. | peptidoglycan recognition proteins (pgrps) can recognize bacterial cell wall (peptidoglycan) and activate innate immune system. in addition to its function as pathogen recognition receptors (prrs), pgrps are also involved in directly killing bacteria, and regulating multiple signaling pathways. recently, we have reported catfish prrs including nucleotide-binding domain, leucine-rich repeat containing receptors (nlrs), retinoic acid inducible gene i (rig-i) like receptors (rlrs), and toll-like re ... | 2014 | 24814805 |
| characterization and mucosal responses of interleukin 17 family ligand and receptor genes in channel catfish ictalurus punctatus. | interleukin (il) 17 family cytokines are important mediators of mucosal immune responses, tightly regulated by signals from the complex milieu of pathogenic and commensal microbes, epithelial cells and innate and adaptive leukocytes found at tissue barriers. in mammals, il17 ligand expression has been linked not only to protective immunity but also excessive tissue inflammation and damage in the gut and lungs. to better understand the scope and action of the il17 family in channel catfish ictalu ... | 2014 | 24602926 |
| expression of nitric oxide synthase (nos) genes in channel catfish is highly regulated and time dependent after bacterial challenges. | nitric oxide is well known for its roles in immune responses. as such, its synthesizing enzymes have been extensively studied from various species including some teleost fish species. however, the nos genes have not been characterized in channel catfish (ictalurus punctatus). in this study, we identified and characterized three nos genes including one nos1 and two nos2 genes in channel catfish. comparing with the nos genes from other fish species, the catfish nos genes are highly conserved in th ... | 2014 | 24560653 |
| effect of multiple mutations in tricarboxylic acid cycle and one-carbon metabolism pathways on edwardsiella ictaluri pathogenesis. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen causing enteric septicemia of catfish (esc). we have shown recently that tricarboxylic acid cycle (tca) and one-carbon (c1) metabolism are involved in e. ictaluri pathogenesis. however, the effect of multiple mutations in these pathways is unknown. here, we report four novel e. ictaluri mutants carrying double gene mutations in tca cycle (eiδmdhδsdhc, eiδfrdaδsdhc), c1 metabolism (eiδglyaδgcvp), and both tca and c1 metab ... | 2014 | 24418045 |
| comparison of vietnamese and us isolates of edwardsiella ictaluri. | we compared edwardsiella ictaluri from striped catfish in vietnam with us channel catfish isolates. biochemical analyses and sequencing of the 16s rrna gene confirmed that the vietnamese isolates were e. ictaluri. comparison using rep-pcr fingerprinting demonstrated no significant differences between the isolates, but plasmid analysis indicated that the vietnamese isolates grouped into 4 plasmid profiles, each different from the typical pei1 and pei2 plasmid profile found in the us isolates. seq ... | 2013 | 24062549 |
| transferable green fluorescence-tagged pei2 in edwardsiella ictaluri and preliminary investigation of its effects on virulence. | edwardsiella ictaluri is the etiologic agent of enteric septicemia of catfish, which causes substantial losses in catfish aquaculture. to determine pathogen-host interactions, previous studies have used the green fluorescence protein (gfp) gene. here, the pei2 plasmid of e. ictaluri isolate i49 was tagged using a tn10-gfp-kan cassette to create the green fluorescence-expressing derivative i49-gfp. the tn10-gfp-kan insertion site was mapped by plasmid sequencing to 663 bp upstream of open reading ... | 2013 | 23836772 |
| tricarboxylic acid cycle and one-carbon metabolism pathways are important in edwardsiella ictaluri virulence. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen causing enteric septicemia of channel catfish (esc). the disease causes considerable economic losses in the commercial catfish industry in the united states. although antibiotics are used as feed additive, vaccination is a better alternative for prevention of the disease. here we report the development and characterization of novel live attenuated e. ictaluri mutants. to accomplish this, several tricarboxylic acid cycle ... | 2013 | 23762452 |
| pathogen recognition receptors in channel catfish: iii phylogeny and expression analysis of toll-like receptors. | toll-like receptors (tlrs) were the earliest characterized and the most extensively studied pathogen recognition receptors (prrs). the majority of tetrapod tlr orthologs have been found in teleost fish. in addition, a group of "fish-specific" tlrs have been identified. in catfish, a number of tlr-related sequences have been reported, but systematic phylogenetic analyses have not been conducted. in this study, we conducted phylogenetic and comparative analysis of 20 catfish tlr genes against thei ... | 2013 | 23396097 |
| construction and evaluation of an edwardsiella ictaluri fhuc mutant. | edwardsiella ictaluri is a gram-negative facultative intracellular pathogen causing enteric septicemia in channel catfish. iron is an essential micronutrient needed for bacterial virulence, and to acquire iron, many gram-negative bacteria secrete ferric iron chelating siderophores. the ferric hydroxamate uptake (fhu) system consists of four genes (fhuc, fhud, fhub, and fhua), and is involved in the uptake of hydroxamate type siderophores across bacterial membranes. however, the fhu system and it ... | 2013 | 23201245 |
| changes of serum myeloperoxidase and nitric oxide in the early stage of edwardsiella ictaluri infection in channel catfish, ictalurus punctatus (rafinesque). | | 2013 | 23126429 |
| use of bioluminescence mutant screening for identification of edwardsiella ictaluri genes involved in channel catfish (ictalurus punctatus) skin colonization. | initial invasion of the host is the first and vital part of any infection process. we have demonstrated that edwardsiella ictaluri is capable of colonizing and penetrating catfish skin. therefore, a mutant library was constructed by random insertion of the mar2xt7 transposon into the chromosome of e. ictaluri harboring the bioluminescence plasmid pakgfplux1. this library was then screened through a series of three consecutive challenges for mutants showing a decreased ability to colonize the cat ... | 2013 | 23092811 |
| spleen index and mannose-binding lectin levels in four channel catfish families exhibiting different susceptibilities to flavobacterium columnare and edwardsiella ictaluri. | edwardsiella ictaluri and flavobacterium columnare are two bacterial pathogens that affect channel catfish ictalurus punctatus aquaculture. at the catfish genetics research unit (u.s. department of agriculture, agricultural research service), some progress has been made in selectively breeding for resistance to e. ictaluri; however, the susceptibility of these families to f. columnare is not known. our objectives were to obtain baseline information on the susceptibility of channel catfish famili ... | 2012 | 22870893 |
| edwardsiella ictaluri as the causative agent of mortality in cultured nile tilapia. | edwardsiella ictaluri was consistently isolated from the spleens, livers, and head kidneys of diseased nile tilapia oreochromis niloticus from a farm experiencing mortality events in several culture ponds. we describe the first published outbreak of e. ictaluri-induced edwardsiellosis in nile tilapia. pure cultures of the isolated bacteria were characterized both biochemically and molecularly. biochemical analysis was performed using the api-20e and rapid one systems, and antimicrobial susceptib ... | 2012 | 22838078 |
| intraspecific diversity of edwardsiella ictaluri isolates from diseased freshwater catfish, pangasianodon hypophthalmus (sauvage), cultured in the mekong delta, vietnam. | a molecular epidemiology study was conducted on 90 edwardsiella ictaluri isolates recovered from diseased farmed freshwater catfish, pangasianodon hypophthalmus, cultured in the mekong delta, vietnam. thirteen isolates of e. ictaluri derived from diseased channel catfish, ictalurus punctatus, cultured in the usa were included for comparison. all the e.ictaluri isolates tested were found to be biochemically indistinguishable. a repetitive (rep)-pcr using the single (gtg)(5) primer was shown to po ... | 2012 | 22804634 |
| synergies between vaccination and dietary arginine and glutamine supplementation improve the immune response of channel catfish against edwardsiella ictaluri. | channel catfish was used to investigate the enhancement of vaccine efficacy following dietary supplementation with arginine (arg, 4% of diet), glutamine (gln, 2% of diet), or a combination of both. after vaccination against edwardsiella ictaluri, humoral and cellular immune responses, along with lymphoid organ responses were evaluated. e. ictaluri-specific antibody titers in plasma were higher (p < 0.05) in fish fed the supplemented diets compared to those fed the basal diet as early as 7 d post ... | 2012 | 22728565 |
| effect of ichthyophthirius multifiliis parasitism on the survival, hematology and bacterial load in channel catfish previously exposed to edwardsiella ictaluri. | the effect of ichthyophthirius multifiliis (ich) parasitism on survival, hematology and bacterial load in channel catfish, ictalurus punctatus, previously exposed to edwardsiella ictaluri was studied. fish were exposed to e. ictaluri 1 day prior to ich in the following treatments: (1) infected by e. ictaluri and ich at 2,500 theronts/fish; (2) infected by e. ictaluri only; (3) infected by ich at 2,500 theronts/fish only; and (4) non infected control. mortality was significantly higher in fish pr ... | 2012 | 22699968 |
| the effect of adjuvant and microbial challenge on the expression of antimicrobial polypeptides in channel catfish (ictalurus punctatus). | while antimicrobial polypeptides (ampps) are increasingly recognized as one of the most important components of innate immunity, there is very little information in vertebrates that documents their upregulation to levels that are microbicidal in vivo. here we demonstrate that intraperitoneal injection of either freund's complete adjuvant (fca) or live tetrahymena pyriformis (a parasitic ciliate) upregulated ampp expression in channel catfish skin. fca induced significant upregulation of total an ... | 2012 | 22507196 |
| enhanced susceptibility of channel catfish to the bacterium edwardsiella ictaluri after parasitism by ichthyophthirius multifiliis. | bacterium edwardsiella ictaluri and parasite ichthyophthirius multifiliis (ich) are two common pathogens of cultured fish. the objective of this study was to evaluate the susceptibility of channel catfish ictalurus punctatus to e. ictaluri and determine bacterial loads in different fish organs after parasitism by ich. fish received the following treatments: (1) infected by i. multifiliis at 5000 theronts/fish and exposed to e. ictaluri; (2) infected by i. multifiliis alone; (3) exposed to e. ict ... | 2012 | 22397934 |
| modified live edwardsiella ictaluri vaccine, aquavac-esc, lacks multidrug resistance plasmids. | plasmid-mediated antibiotic resistance was first discovered in edwardsiella ictaluri in the early 1990s, and in 2007 an e. ictaluri isolate harboring an inca/c plasmid was recovered from a moribund channel catfish ictalurus punctatus infected with the bacterium. due to the identification of multidrug resistance plasmids in aquaculture and their potential clinical importance, we sought to determine whether the modified live e. ictaluri vaccine strain in aquavac-esc harbors such plasmids, so that ... | 2011 | 22372247 |
| a real-time polymerase chain reaction assay for quantification of edwardsiella ictaluri in catfish pond water and genetic homogeneity of diagnostic case isolates from mississippi. | a quantitative polymerase chain reaction (qpcr) assay was developed for the detection and quantification of edwardsiella ictaluri in channel catfish ictalurus punctatus pond water using modifications to a published e. ictaluri-specific qpcr assay and previously established protocols for the molecular detection of myxozoan parasites in catfish ponds. genomic dna equivalents indicative of the number of bacteria in a sample were determined and standard curves correlating to bacterial numbers were e ... | 2011 | 22372245 |
| global transcription analysis of vaccinated channel catfish following challenge with virulent edwardsiella ictaluri. | to determine the identities of genes involved in either innate or adaptive immunity, microarray analysis of 65,182 unigene transcripts were performed to compare gene expression in vaccinated channel catfish after challenge with a virulent edwardsiella ictaluri compared to that in sham-vaccinated fish without challenge. with a filter of false-discovery rate less than 0.05 and fold change greater than 2, a total of 167 functionally known unique transcripts were found to be up-regulated, whereas 40 ... | 2012 | 22365332 |
| arginine and glutamine supplementation to culture media improves the performance of various channel catfish immune cells. | specific components of both the innate and adaptive immune systems of channel catfish were evaluated after supplementation of culture media with arginine (arg) and/or glutamine (gln). primary cell cultures of head-kidney macrophages (mø) were used for phagocytic and bactericidal assays against edwardsiella ictaluri. additionally, proliferation assays were conducted with naïve peripheral blood lymphocytes (pbl) exposed to non-specific mitogens. to indirectly assess amino acid utilization of both ... | 2012 | 22326940 |
| expression profiles of seven channel catfish antimicrobial peptides in response to edwardsiella ictaluri infection. | using quantitative polymerase chain reaction (qpcr), the relative transcriptional levels of seven channel catfish antimicrobial peptide (amp) genes (nk-lysin type 1, nk-lysin type 2, nk-lysin type 3, bactericidal permeability-increasing protein, cathepsin d, hepcidin and liver-expressed amp 2) in response to edwardsiella ictaluri infection were determined. none of the amp genes tested was significantly upregulated at 2 h post-infection. hepcidin was the only one that was significantly (p<0.05) u ... | 2012 | 22324346 |
| ichthyophthirius multifiliis as a potential vector of edwardsiella ictaluri in channel catfish. | there is limited information on whether parasites act as vectors to transmit bacteria in fish. in this trial, we used ichthyophthirius multifiliis and fluorescent edwardsiella ictaluri as a model to study the interaction between parasite, bacterium, and fish. the percentage (23-39%) of theronts fluorescing after exposure to e. ictaluri was significantly higher than control theronts (~ 6%) using flow cytometry. theronts exposed to e. ictaluri at 4 × 10(7) cfu ml(-1) showed a higher percentage (~ ... | 2012 | 22309484 |
| global gene expression in channel catfish after vaccination with an attenuated edwardsiella ictaluri. | to understand the global gene expression in channel catfish after immersion vaccination with an attenuated edwardsiella ictaluri (aquavac-esc™), microarray analysis of 65,182 unigene transcripts was performed. with a filter of false-discovery rate less than 0.05 and fold change greater than 2, a total of 52 unique transcripts were found to be upregulated in vaccinated fish at 48 h post vaccination, whereas a total of 129 were downregulated. the 52 upregulated transcripts represent genes with put ... | 2012 | 22245589 |
| complete genome sequence of edwardsiella tarda isolate fl95-01, recovered from channel catfish. | edwardsiella tarda is a gram-negative facultative anaerobe that has been isolated from fish, reptiles, amphibians, and mammals, including humans. this is a report of the complete and annotated genome of isolate fl95-01, recovered from channel catfish (ictalurus punctatus). | 2015 | 26112788 |
| real-time polymerase chain reaction assays for the detection and quantification of edwardsiella tarda, edwardsiella piscicida, and edwardsiella piscicida-like species in catfish tissues and pond water. | researchers have proposed the adoption of 3 distinct genetic taxa among bacteria previously classified as edwardsiella tarda; namely e. tarda, e. piscicida, and a taxon presently termed e. piscicida-like. individual real-time polymerase chain reaction (qpcr) assays were developed, based on published primers, for e. tarda, e. piscicida, and e. piscicida-like sp. to provide rapid quantitative confirmatory tests for these phenotypically ambiguous bacteria. the qpcr assays were shown to be repeatabl ... | 2015 | 25613040 |
| complete genome sequence of channel catfish gastrointestinal septicemia isolate edwardsiella tarda c07-087. | edwardsiella tarda is a gram-negative facultative anaerobe causing disease in animals and humans. here, we announce the complete genome sequence of the channel catfish isolate e. tarda strain c07-87, which was isolated from an outbreak of gastrointestinal septicemia on a commercial catfish farm. | 2013 | 24265493 |
| class ii, major histocompatibility complex, transactivator (ciita) in channel catfish: identification and expression patterns responding to different pathogens. | nlrs are a large family belonging to pattern recognition receptors which could recognize pathogen associated molecular patterns. class ii, major histocompatibility complex, transactivator (ciita) is a member of nlr family. it is a critical transcription factor which could regulate the expression of mhc class ii. in this study, a full-length cdna of ciita was cloned from channel catfish according to ten sequenced ests. this cdna contains a 5'-utr of 71 bp, a 3'-utr of 238 bp and an orf of 3,210 b ... | 2012 | 23073771 |
| birc7 gene in channel catfish (ictalurus punctatus): identification and expression analysis in response to edwardsiella tarda, streptococcus iniae and channel catfish hemorrhage reovirus. | a family member of inhibitor of apoptosis protein (iap) termed baculoviral iap repeat-containing 7 (birc7) from channel catfish (ictalurus punctatus) was identified, the full length cdna sequence of channel catfish birc7 (ccbirc7) was 1686 bp, containing a 5'utr of 93 bp, a 3'utr of 399 bp with a poly (a) tail and an orf of 1194 bp encoding a putative protein of 398 amino acids. the putative ccbirc7 protein contains two bir super-family conservative domains and a c-terminal ring finger motif. ph ... | 2012 | 22510211 |
| a loop-mediated isothermal amplification method for the detection of members of the genus ranavirus. | a loop-mediated isothermal amplification (lamp) method was developed for detection of members of the genus ranavirus. the optimum reaction mixture contained 2.5 μl of each inner primer, rv-fip (20 pmol/μl) and rv-bip (20 pmol/μl), 0.5 μl of each outer primer, rv-f3 (10 pmol/μl) and rv-b3 (10 pmol/μl), 1.25 μl of each loop primer, rv-lf (20 pmol/μl) and rv-lb (20 pmol/μl), 3.5 μl dntp mix (10 mm each), 8 μl mgso4 (25 mm), 1 μl of bst dna polymerase (8 u/ml, large fragment; new england biolabs inc ... | 2013 | 23665768 |
| expression, purification and antibacterial activity of the channel catfish hepcidin mature peptide. | hepcidins are small cysteine-rich cationic antimicrobial peptides. the channel catfish (ictalurus punctatus) hepcidin cdna has been characterized, but recombinant protein expression and purification was not reported. i. punctatus hepcidin is comprised of 96 residues, with eight functionally important conserved cysteine residues located in the c-terminal region of the mature peptide, suggesting that this region is responsible for the antibacterial activity. in this study, a cdna fragment (mch) en ... | 2014 | 24269761 |
| identification of envelope protein orf10 of channel catfish herpesvirus. | channel catfish virus (ccv) is a viral pathogen of fry and fingerling channel catfish and can cause significant commercial loss. previous studies have shown that the ccv virion contains at least 25 predicted structural proteins, including viral protein 10, which is encoded by the orf10 gene of the ccv. in this paper, the orf10 gene was expressed in escherichia coli and used to produce a specific antibody. western blot analysis confirmed that open reading frame 10 is an envelope protein. a viral ... | 2012 | 22356443 |
| molecular characterization of the insulin-like growth factor-i (igf-i) gene in channel catfish (ictalurus punctatus). | the insulin-like growth factor i (igf-i) gene was characterized in channel catfish. partial cdna sequence, missing exon 1 and part of exon 2, was obtained in 5'- and 3'-race experiments. direct sequencing of two bacterial artificial chromosome clones revealed gene structure and provided sequence from 640 bp upstream of the initiator methionine to 136 bp beyond the polyadenylation site. genomic sequence contained a putative tata box 506 bp upstream of the initiator methionine. the 477-bp reading ... | 2005 | 16298440 |
| identification of nf-κb related genes in channel catfish and their expression profiles in mucosal tissues after columnaris bacterial infection. | interactions of nf-κb family, iκb family and ikk complex are the key components of nf-κb pathway that is essential for many biological processes including innate and adaptive immunity, inflammation and stress responses. in spite of their importance, systematic analysis of these genes in fish has been lacking. here we report a systematic study of the nf-κb related genes in channel catfish. five nf-κb family genes, five iκb family genes and three ikk complex genes were identified in the channel ca ... | 2017 | 28063885 |
| impact of oral and waterborne administration of rhamnolipids on the susceptibility of channel catfish (ictalurus punctatus) to flavobacterium columnare infection. | flavobacterium columnare is the causative agent of columnaris disease and causes tremendous morbidity and mortality of farmed fish globally. previously, we identified a potential lectin-mediator (a rhamnose-binding lectin; rbl1a) of f. columnare adhesion and showed higher rbl1a expression in susceptible channel catfish under basal conditions and following infection. exposure of challenged fish to the carbohydrate ligand l-rhamnose just prior to a challenge substantially decreased columnaris mort ... | 2017 | 27818340 |
| genome sequence of the fish pathogen flavobacterium columnare genomovar ii strain 94-081. | flavobacterium columnare causes columnaris disease in fresh and brackish water worldwide. f. columnare strain 94-081 was isolated from a diseased channel catfish in 1994; its genome sequence is the first completed genomovar ii sequence. | 2016 | 27231366 |
| missing the target: dnak is a dominant epitope in the humoral immune response of channel catfish (ictalurus punctatus) to flavobacterium columnare. | vaccination remains a viable alternative for bacterial disease protection in fish; however additional work is required to understand the mechanisms of adaptive immunity in the channel catfish. to assess the humoral immune response to flavobacterium columnare; a group of channel catfish were first immunized with f. columnare lv-359-01 cultured in iron-depleted media, before being challenged with wild type f. columnare lv-359-01. the immunization protocol did not confer increased protection agains ... | 2016 | 26892797 |
| the use of phage fcl-2 as an alternative to chemotherapy against columnaris disease in aquaculture. | flavobacterium columnare, the causative agent of columnaris disease in fish, causes millions of dollars of losses in the us channel catfish industry alone, not to mention aquaculture industry worldwide. novel methods are needed for the control and treatment of bacterial diseases in aquaculture to replace traditionally used chemotherapies. a potential solution could be the use of phages, i.e., bacterial viruses, host-specific and self-enriching particles that can be can easily distributed via wat ... | 2015 | 26347722 |
| protective efficacy of nigella sativa seeds and oil against columnaris disease in fishes. | columnaris disease, caused by the bacterium flavobacterium columnare, is currently the most frequently reported bacterial disease affecting farm-raised channel catfish in the usa. common treatments against the disease include the use of medicated feed that has led to emergent antibiotic resistant strains of f. columnare. nigella sativa (black cumin) is a medicinal herb commonly used by many cultures as a natural remedy for numerous disorders. recently, we have discovered the antibacterial activi ... | 2016 | 26265495 |
| expression profiling analysis of immune-related genes in channel catfish (ictalurus punctatus) skin mucus following flavobacterium columnare challenge. | fish are covered by a watery gel-mucus, mainly secreted by the goblet cells, serving as the physical and biochemical barrier between the external environment and the interior milieu, playing more important roles in fish that without scale. despite the important roles of mucus in fish immunity, the knowledge of detailed molecular events happened during infection process is still limited. while most studies were focused on characterizing the protein and enzyme activities in the mucus following cha ... | 2015 | 26220643 |
| a comparison of high- and low-virulence flavobacterium columnare strains reveals differences in iron acquisition components and responses to iron restriction. | flavobacterium columnare, the causative agent of columnaris disease causes substantial mortality worldwide in numerous freshwater finfish species. due to its global significance, an improved understanding of the factors that contribute to virulence is urgently needed. in a laboratory challenge, we found that significantly greater mortality was observed in channel catfish ictalurus punctatus (rafinesque) challenged with isolate lsu-066-04 (lsu) as compared to fish challenged with isolate lv-359-0 ... | 2016 | 25704170 |
| activities of wogonin analogs and other flavones against flavobacterium columnare. | in our on-going pursuit to discover natural products and natural product-based compounds to control the bacterial species flavobacterium columnare, which causes columnaris disease in channel catfish (ictalurus punctatus), we synthesized flavone and chalcone analogs, and evaluated these compounds, along with flavonoids from natural sources, for their antibacterial activities against two isolates of f. columnare (alm-00-173 and biomed) using a rapid bioassay. the flavonoids chrysin (1a), 5,7-dihyd ... | 2015 | 25676507 |
| epidemiology of columnaris disease affecting fishes within the same watershed. | in the southeastern usa, columnaris disease (caused by flavobacterium columnare) typically affects catfish raised in earthen ponds from early spring until late summer. recently, unusually severe outbreaks of columnaris disease occurred at the e. w. shell fisheries center located in auburn, al, usa. during these outbreaks, catfish and other aquaculture and sport fish species that were in ponds located within the same watershed were affected. our objective was to investigate the genetic diversity ... | 2014 | 24991846 |
| kaolinitic clay protects against flavobacterium columnare infection in channel catfish ictalurus punctatus (rafinesque). | columnaris disease, caused by the bacterial pathogen flavobacterium columnare, continues to be a major problem worldwide in both wild and cultured freshwater finfish. despite the far-reaching negative impacts of columnaris disease, safe and efficacious preventatives and curatives for this disease remain limited. in this study, we evaluated the potential of kaolin (al2 si2 05 (oh)4 ), a type of clay, for the prevention of columnaris disease. channel catfish, ictalurus punctatus (rafinesque), fing ... | 2015 | 24499204 |
| l-rhamnose-binding lectins (rbls) in channel catfish, ictalurus punctatus: characterization and expression profiling in mucosal tissues. | rhamnose-binding lectins (rbls) have recently emerged as important molecules in the context of innate immunity in teleost fishes. previously, using rna-seq technology, we observed marked up-regulation of a rbl in channel catfish (ictalurus punctatus) gill following a challenge with the bacterial pathogen flavobacterium columnare. furthermore, the magnitude of rbl up-regulation positively correlated with disease susceptibility. moving forward from these findings, we wished to more broadly underst ... | 2014 | 24480296 |
| nutritional impacts on gene expression in the surface mucosa of blue catfish (ictalurus furcatus). | short-term feed deprivation is a common occurrence in both wild and farmed fish species, due to reproductive processes, seasonal variations in temperature, or in response to a disease outbreak. fasting can have dramatic physiological and biological consequences for fish, including impacts on mucosal immunity which can, in turn, change host susceptibility to pathogens. culture and selection of blue catfish (ictalurus furcatus) has gained importance as the production of a channel catfish×blue catf ... | 2014 | 24378224 |
| short-term feed deprivation alters immune status of surface mucosa in channel catfish (ictalurus punctatus). | short-term feed deprivation (or fasting) is a common occurrence in aquacultured fish species whether due to season, production strategies, or disease. in channel catfish (ictalurus punctatus) fasting impacts susceptibility to several bacterial pathogens including flavobacterium columnare, the causative agent of columnaris disease. as columnaris gains entry through the gills and skin of fish, we examined here changes in transcriptional regulation induced in these surface mucosal tissues due to sh ... | 2013 | 24023952 |
| new attenuated vaccine against columnaris disease in fish: choosing the right parental strain is critical for vaccine efficacy. | flavobacterium columnare, the causative agent of columnaris disease, is a highly diverse species comprised by three genomovars. genomovar ii strains are more virulent toward catfishes than genomovar i isolates. the objective of this study was to compare the vaccine efficacy of avirulent mutants derived from genomovars i and ii using a rifampicin-resistance strategy. first, we compared the efficacy of 13 genomovar ii mutants in channel catfish (ictalurus punctatus) fingerlings and identified muta ... | 2013 | 24012568 |
| basal polarization of the mucosal compartment in flavobacterium columnare susceptible and resistant channel catfish (ictalurus punctatus). | the freshwater bacterial pathogen, flavobacterium columnare, infects a variety of ornamental and farmed fish species worldwide through mucosal attachment points on the gill and skin. while previous studies have demonstrated a chemotactic response of f. columnare to fish mucus, little is known about how host gill mucosal molecular and cellular constituents may impact rates of adhesion, tissue invasion, and ultimately, mortality. here, we describe the use of rna-seq to profile gill expression diff ... | 2013 | 23895942 |
| biofilm formation by the fish pathogen flavobacterium columnare: development and parameters affecting surface attachment. | flavobacterium columnare is a bacterial fish pathogen that affects many freshwater species worldwide. the natural reservoir of this pathogen is unknown, but its resilience in closed aquaculture systems posits biofilm as the source of contagion for farmed fish. the objectives of this study were (i) to characterize the dynamics of biofilm formation and morphology under static and flow conditions and (ii) to evaluate the effects of temperature, ph, salinity, hardness, and carbohydrates on biofilm f ... | 2013 | 23851087 |
| ungeremine and its hemisynthesized analogues as bactericides against flavobacterium columnare. | the gram-negative bacterium flavobacterium columnare is the cause of columnaris disease, which can occur in channel catfish ( ictalurus punctatus ). in a previous study, the betaine-type alkaloid ungeremine, 1, obtained from pancratium maritimum l. was found to have strong antibacterial activity against f. columnare. in this study, analogues of 1 were evaluated using a rapid bioassay for activity against f. columnare to determine if the analogues might provide greater antibacterial activity and ... | 2013 | 23331165 |
| putative roles for a rhamnose binding lectin in flavobacterium columnare pathogenesis in channel catfish ictalurus punctatus. | columnaris disease, caused by the bacterial pathogen flavobacterium columnare, continues to be a major problem worldwide and commonly leads to tremendous losses of both wild and cultured freshwater fish, particularly in intensively farmed aquaculture species such as channel catfish. despite its ecologic and economic impacts, the fundamental molecular mechanisms of the host immune response to this pathogen remain unclear. while f. columnare can induce marked pathologic changes in numerous ectopic ... | 2012 | 22960031 |
| antimicrobial susceptibility pattern of flavobacterium columnare isolates collected worldwide from 17 fish species. | flavobacterium columnare is the causative agent of columnaris disease in diverse fish species worldwide. although columnaris is an important disease, the antimicrobial susceptibility pattern of f. columnare is not well studied. thus, the purpose of this study was to test the in vitro antimicrobial susceptibility of 97 f. columnare isolates collected worldwide between 1987 and 2011 from 17 fish species. the broth microdilution technique was utilized for reliable testing of these fastidious organi ... | 2013 | 22957716 |
| pond-level risk factors associated with columnaris disease on mississippi commercial catfish farms. | a large commercial catfish enterprise encompassing over 500 food fish ponds from five farms covering multiple counties in the mississippi delta was included in this analysis of columnaris risk factors. a gram-negative bacterium, flavobacterium columnare, is the cause of columnaris disease and is considered the second-most prevalent bacterial disease in farm-raised catfish. the objective of this study was to determine if pond-level risk factors reported by farm personnel were associated with colu ... | 2012 | 22897761 |