| a study of porcine lymphocyte populations. i. separation of porcine lymphocyte subpopulations. | several methods for separating porcine lymphocyte populations were studied. lymphocyte populations enriched in t cells were obtained by filtration on nylon fiber columns. lymphocyte populations enriched in b cells were obtained by e rosette sedimentation. results obtained with anti-immunoglobulin columns were less satisfactory. attempts to fractionate porcine lymphoid cells with the help of helix pomatia lectin remained unsuccessful. | 1980 | 15615056 |
| detection of synthetic glucocorticoid residues in cattle tissue and hair samples after a single dose administration using lc-ms/ms. | a sensitive and specific liquid chromatography-tandem mass spectrometry (lc-ms/ms) assay for the detection of several synthetic glucocorticoids in kidney, muscle and hair samples of cattle after a single intramuscular injection is described. after a dichloromethane wash of the hair samples, analytes were released from the hair matrix by enzymatic digestion. muscle samples were also digested enzymatically using proteinase, while kidney samples were deconjugated by helix pomatia juice. these preli ... | 2005 | 15686988 |
| supercooling ability in two populations of the land snail helix pomatia (gastropoda: helicidae) and ice-nucleating activity of gut bacteria. | the land snail helix pomatia (gastropoda: helicidae) is widely distributed in northern and central europe where it may experience subzero temperatures during winter months. its supercooling ability was studied in two populations of h. pomatia. one population originated from southern sweden (gotaland) and the other from central france (auvergne). in the experimental design, they were acclimated, over 2 weeks, to artificial winter conditions (hibernation, t=5 degrees c). the swedish snails showed ... | 2004 | 15710369 |
| cancer metastasis: characterization and identification of the behavior of metastatic tumor cells and the cell adhesion molecules, including carbohydrates. | this review focuses on the behavior of metastatic tumor cells and their specific adhesion molecules. much of this review is based on the results from our researches over many years. electron microscopic investigations of metastatic processes have demonstrated that desmosomes, tight junctions, or cell fusion-like structures are formed between tumor cells and other cells such as endothelial, mesothelial, hepatic, and nerve cells. these findings suggest that metastatic tumor cells acquire specific ... | 2005 | 15720223 |
| enrichment of cheeses manufactured from cow's and sheep's milk blends with sheep-like species-related alkylphenols. | enhancement of concentrations of species-related sheep-like alkylphenols, p- and m-cresols and 3- and 4-ethylphenols, in experimental manchego-type cheeses manufactured from cow's and sheep's milk blends (80:20) by using arylsulfatases was investigated. a food-grade arylsulfatase from aspergillus oryzae (atcc 20719) was produced using a stimulatory medium, and crude dried cells were used as the enzyme source. exogenous arylsulfatases from helix pomatia and a. oryzae were added to cheese curd, an ... | 2005 | 15740062 |
| the tidemark of the chondro-osseous junction of the normal human knee joint. | the chondro-osseous junction includes the junction between calcified and non-calcified cartilage matrices often referred to as the tidemark. a detailed knowledge of the structure, function and pathophysiology of the chondro-osseous junction is essential for an understanding both of the normal elongation of bones and of the pathogenesis of osteoarthrosis. in this study the molecular anatomy of the tidemark was studied using histochemical techniques, including lectin histochemistry, on blocks of n ... | 2005 | 15900412 |
| optimization of conditions for the enzymatic hydrolysis of phytoestrogen conjugates in urine and plasma. | optimal ph, temperature, and concentration of enzyme conditions for the rate of hydrolysis of five isoflavone conjugates (daidzein, o-desmethylangolensin, equol, genistein, and glycitein) and two lignans (enterodiol and enterolactone) from two biological matrices (urine and plasma) were studied using beta-glucuronidase from helix pomatia. in addition, the use of mixtures of beta-glucuronidase and sulfatase enzymes from different sources was investigated to find enzyme preparations that contained ... | 2005 | 15907867 |
| gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine. | a sensitive and efficient method was developed for the determination of carvedilol and its metabolites in human urine by gas chromatography-mass spectrometry (gc-ms). urine samples were hydrolyzed with beta-glucuronidase/arylsulfatase (from helix pomatia) and the target compounds were extracted with liquid-liquid extraction. the extracts were completely derivatized with mstfa and mbtfa and analyzed by gc-ms using an ultra-2 column. the linearity of the assay ranges were 0.75-75 ngml(-1) for carv ... | 2005 | 15996536 |
| the lectin helix pomatia agglutinin as a marker of metastases--clinical and experimental studies. | the prognosis for the majority of patients suffering from a solid neoplasm remains bleak, due to the fact that most cancers have already formed metastases at distant sites. any progress in cancer therapy will, therefore, depend on the understanding of the metastatic cascade and therapies derived from this understanding. the development of clinically relevant models of metastases, using lectin-defined human cancer cell lines and severe combined immunodeficient (scid) mice, is described. in conjun ... | 2005 | 16033108 |
| hydrolysis of conjugated metabolites of buprenorphine ii. the quantitative enzymatic hydrolysis of norbuprenorphine-3-beta-d-glucuronide in human urine. | in gas chromatographic-mass spectroscopic analysis of buprenorphine metabolites, the urine specimen must be first hydrolyzed to release buprenorphine and norbuprenorphine from their glucuronide conjugates. for evaluation of existing hydrolysis methods and to find out the optimal hydrolysis conditions, buprenorphine-3-beta-d-glucuronide (b3g) and norbuprenorphine-3-beta-d-glucuronide (nb3g) were synthesized. in a previous communication we reported on the optimum conditions for hydrolysis of b3g. ... | 2005 | 16168181 |
| membrane mechanisms of antiarrhythmic effect of quaternidine. | complex electrophysiological study of the effects of quaternidine carried out on intact hearts from cats, myocardial fragments from rats, and single ionic channels of large edible snail showed that quaternidine demonstrates properties of class 1b antiarrhythmic drug according to vaughan-williams nomenclature. this agent did not suppress nomotopic pacemaker automaticity, did not change conduction in ventricles, atria, and atrioventricular junction in hearts with preserved sinus rhythm, did not pr ... | 2005 | 16224582 |
| evidence for the cd(2+) activation of the aryl sulfatase from helix pomatia. | often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from helix pomatia remain little characterized. as many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the sulfatase was investigated. evidence for metal ion activation was collected, with cd(2+) being notable for effective activation. the enzyme was inhibited by cu(2+). the response of other common hydrolases to divalent metal ions w ... | 2005 | 16333754 |
| purification of the crude solution from helix pomatia for use as beta-glucuronidase and aryl sulfatase in phytoestrogen assays. | phytoestrogens occur in a variety of foods and are thought to offer a protective effect against a number of complex diseases. due to the diversity of phytoestrogen conjugates formed in the human body, most assays include an enzymatic hydrolysis step prior to analysis. beta-glucuronidase from helix pomatia, which also contains sulfatase activity, is popular for this task but contains appreciable levels of some phytoestrogens and related compounds, which could affect accurate quantification at low ... | 2006 | 16388994 |
| isolation and characterization of metabolically competent pulmonary epithelial cells from pig lung tissue. | administration of drugs by inhalation opens new possibilities for entry into the systemic circulation and cultures of porcine pulmonary epithelial cells (pecs) may prove to be valuable in the prediction of pulmonary metabolism of drugs in humans. this paper, therefore, reports a method for the routine isolation and cultivation of pecs from slaughterhouse animals. on average 1.5x10(6) cells g-1 tissue were isolated by discontinuous density-gradient centrifugation. cells were subsequently cultivat ... | 2005 | 16393853 |
| in vivo xenograft models of breast cancer metastasis. | if breast cancer patients are not cured, it is largely because of the fact that the cancer has spread beyond its primary site--the breast--to distant sites, such as, e.g., bone marrow, lung, brain, and/or liver. these secondary tumors are called metastases, and the underlying mechanisms leading to these secondary tumor deposits are complex and still ill understood. in this chapter, we report on how to develop clinically relevant human breast cancer cell line xenografts in severe combined immunod ... | 2006 | 16491620 |
| the effect of lectins on cryptosporidium parvum oocyst in vitro attachment to host cells. | the influence of lectins on cryptosporidium parvum oocyst agglutination and on attachment to both fixed madin darby canine kidney (mdck) cells and fixed hct-8 (human colorectal epithelial) cells was examined. oocyst cell wall characteristics were examined by transmission electron microscopy. lectin-free oocysts were shown to adhere equally to both mdck cells and hct-8 cells. in mdck cells, the addition of 1-25 microg/ml codium fragile lectin, 10 microg/ml maclura pomifera lectin, 10 microg/ml he ... | 2006 | 16629306 |
| alternative methodology for the analysis of progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle. | research has shown that traditional solvent extraction procedures used for the analysis of endogenous steroids often give inconsistent recoveries and results. however, a single-laboratory validation of a liquid chromatography/tandem mass specrometry method using 2 product ions per transition for progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle showed accuracy and precision to within 23% at concentrations ranging from 0.5 to 2.0 microg/kg. homogenized samples were ... | 2006 | 16640309 |
| biochemical and structural analysis of helix pomatia agglutinin. a hexameric lectin with a novel fold. | helix pomatia agglutinin (hpa) is a n-acetylgalactosamine (galnac) binding lectin found in the albumen gland of the roman snail. as a constituent of perivitelline fluid, hpa protects fertilized eggs from bacteria and is part of the innate immunity system of the snail. the peptide sequence deduced from gene cloning demonstrates that hpa belongs to a family of carbohydrate-binding proteins recently identified in several invertebrates. this domain is also present in discoidin from the slime mold di ... | 2006 | 16704980 |
| the use of lectins to characterize and separate living canine spermatozoa: a preliminary study. | this study was designed as a preliminary attempt to develop a methodology for relating the glucidic structure of the sperm membrane to sperm morphology. differences in plasma membrane glycoconjugates between motile and nonmotile spermatozoa were studied by using 7 lectins. fresh spermatozoa from 3 dogs were analyzed by fluorescein isothiocyanate (fitc) labeled lectins. the binding of lectins to the sperm membrane and the capability of the lectins to agglutinate spermatozoa were estimated semi-qu ... | 1994 | 16727393 |
| [comparative analysis of map/erk-kinase activation in the cns of animals with different capability for learning]. | with the western-blot analysis, we studied the activation of map/erk protein kinases (regulating genes expression by the phosphorylation of transcription factors (tf) creb and elk-1) in native helix pomatia and in animals with disturbed abilities to form long-term conditioned avoidance reflexes. different stages of this reflex formation were shown to be characterized by different levels of map/erk kinases activation, the latter being different in cns's regions (visceral complex of ganglia, cereb ... | 2006 | 16869282 |
| functional interaction of escherichia coli heat-labile enterotoxin with blood group a-active glycoconjugates from differentiated ht29 cells. | human colon adenocarcinoma cells (ht29-atcc) and the clone ht29-5f7 were cultured under conditions that differentiate cells to a polarized intestinal phenotype. differentiated cells showed the presence of junctional complexes and intercellular lumina bordered by microvilli. intestinal brush border hydrolase activities (sucrase, aminopeptidase n, lactase and maltase) were detected mainly in differentiated ht29-atcc cells compared with the differentiated clone, ht29-5f7. the presence of non-gm1 re ... | 2006 | 16884490 |
| an accurate and reproducible method for the quantitative analysis of isoflavones and their metabolites in rat plasma using liquid chromatography/mass spectrometry combined with photodiode array detection. | to study the safety and potential health benefits of soy isoflavones, a rapid and simple method based on liquid chromatography combined with mass spectrometry (lc/ms) and photodiode array detector (pda) was developed for the determination of isoflavones in rat plasma. the analytes included daidzein, genistein, glycitein, equol, 4-ethyl phenol, and biochanin a over a concentration range of 1.0-4320.0 nm using 75 microl of rat plasma. rat plasma samples were hydrolyzed by adding an enzyme mixture ... | 2006 | 16915859 |
| structure and in vitro cytocompatibility of the gastropod shell of helix pomatia. | distinguishing features of biological constructions are high stability and adaptation to their environment. beside biocompatibility, nontoxicity and degradability these characteristics are demanded for new biomaterials in the field of tissue engineering. this study investigated the chemical composition, the organization and the in vitro osteoconductive potential of the terrestrial gastropod shell (helix pomatia) on cal72 and human osteoblast-like cells. chemical composition of the biomaterial wa ... | 2006 | 17010402 |
| assessing the instability of the isoelectric focusing patterns of erythropoietin in urine. | ief can be used to differentiate human urinary erythropoietin (uepo), recombinant human erythropoietin or epoetin (repo) and darbepoetin (novel erythropoiesis stimulating protein (nesp)). this is the basis of the method currently used to detect misuse of repo and nesp by elite athletes. recently, an unknown activity has been attributed to some urine samples (denominated 'unstable' urine by the world anti-doping agency; wada). this activity has shown to give rise to artefactual profiles for both ... | 2006 | 17054082 |
| glycoproteome profiling of transforming growth factor-beta (tgfbeta) signaling: nonglycosylated cell death-inducing dff-like effector a inhibits tgfbeta1-dependent apoptosis. | transforming growth factor-beta (tgfbeta) is a potent regulator of cell growth, differentiation, and apoptosis. tgfbeta binds to specific serine/threonine kinase receptors, which leads to activation of smad-dependent and smad-independent signaling pathways. o-glycosylation is a dynamic ptm which has been observed in many regulatory proteins, but has not been studied in the context of tgfbeta signaling. to explore the effect of tgfbeta1 on protein o-glycosylation in human breast epithelial cells, ... | 2006 | 17080483 |
| selection and optimization of hydrolysis conditions for the quantification of urinary metabolites of mdma. | recovery of 3,4-methylenedioxymethamphetamine (mdma) urinary metabolites requires optimization of the hydrolysis of 4-hydroxy-3-methyoxymethamphetamine (hmma), 4-hydroxy-3-methoxyamphetamine (hma), and 3,4-methylenedioxyamphetamine (mda) conjugates prior to chromatographic analysis. acidic and enzymatic hydrolysis with beta-glucuronidase from escherichia coli and helix pomatia were evaluated. acid hydrolysis yielded 40.0% and 39.3% higher hma recovery compared to e. coli and h. pomatia hydrolysi ... | 2006 | 17132253 |
| systematic analysis of animals used in medieval azerbaijan medicine. | in order to study the special composition of animals used in the medieval medicine of azerbaijan, a wide range of medieval sources on medicine and pharmacognosy from the collection of the institute of manuscripts of the azerbaijan academy of sciences in baku has been studied. about 40 medieval sources from the 10-18th centuries including 17 manuscripts in turkic, persian and arabic have been selected as the objects of this study. as a result, 150 species of animals described in medieval azerbaij ... | 2006 | 17153727 |
| the c-type lectin mgl expressed by dendritic cells detects glycan changes on muc1 in colon carcinoma. | the epithelial mucin muc1 is a high molecular weight membrane glycoprotein frequently overexpressed and aberrantly glycosylated in adenocarcinoma. mucins normally contain high amounts of o-linked carbohydrate structures that may influence immune reactions to this antigen. during malignant transformation, certain glyco-epitopes of muc1, such as tn-antigen, tf-antigen and their sialylated forms become exposed. the role of these glycan structures in tumor biology is unknown, but their presence is k ... | 2007 | 17195076 |
| stable expression of cryptosporidium parvum glycoprotein gp40/15 in toxoplasma gondii. | cryptosporidium is a cause of diarrheal disease worldwide. parasite glycoproteins involved in invasion of cryptosporidium into host cells have been investigated as possible targets for effective interventions against this parasite. one of these, cpgp40/15, is expressed as a precursor protein that is cleaved by a parasite-derived furin-like protease activity into gp15, a glycophosphatidyl inositol anchored surface protein, and gp40, that associates with gp15 and binds to host cells. investigation ... | 2007 | 17275106 |
| reactivities of n-acetylgalactosamine-specific lectins with human iga1 proteins. | lectins are proteins with specificity of binding to certain monosaccharides or oligosaccharides. they can detect abnormal glycosylation patterns on immunoglobulins in patients with various chronic inflammatory diseases, including rheumatoid arthritis and iga nephropathy (igan). however, lectins exhibit binding heterogeneity, depending on their source and methods of isolation. to characterize potential differences in recognition of terminal n-acetylgalactosamine (galnac) on iga1, we evaluated the ... | 2007 | 17275907 |
| [activation of cardiac contractivity of the edible snail h. pomatia under effect of thrombin. study of role of camp]. | thrombin acts on mammalian cells through specific, the so-called protease-activated receptors (pars). the thrombin action is mediated via three out of four known types of these receptors par(1, 3, 4). mammalian thrombin receptors, apart from performance of other functions, control cardiac and vascular contractility. it is not known whether receptors of such kind exist in invertebrate animals. in the present work we have showed for the first time that thrombin in the concentration range of 0.01-1 ... | 2007 | 17408090 |
| detection of glucocorticoid residues in pig liver by high-performance liquid chromatography with on-line electrogenerated [cu(hio6)2](5-)--luminol chemiluminescence detection. | a novel method was developed for the simultaneous determination of glucocorticoid residues such as triamcinolone (tr), prednisolone (pr), hydrocortisone (hc), cortisone (co), methylprednisolone (mp), dexamethasone (de) and triamcinolone acetonide (ta) by high-performance liquid chromatography (hplc) coupled with chemiluminescence (cl) detection. the procedure was based on the enhancement effect of glucocorticoids on the chemiluminescence reaction between luminol and the complex of trivalent copp ... | 2007 | 17449041 |
| tandem exploitation of helix pomatia glycosyltransferases: facile syntheses of h-antigen-bearing oligosaccharides. | snails from the family helicidae produce in their albumen glands a highly branched galactan, which consists almost exclusively of d- and l-galactose. the d-gal residues are glycosydically beta(1-->6)- or beta(1-->3)-linked, whereas the l-gal moieties are attached alpha(1-->2). up until the present time, two beta(1-->6)-d-galactosyl transferases and one alpha(1-->2)-l-galactosyl transferase have been identified in a membrane preparation of these glands. these were used to synthesise various oligo ... | 2007 | 17591726 |
| isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata). | the intestinal microbiota of the edible snails cornu aspersum fsyn: h. aspersa), and helix pomatia were investigated by culture-based methods, 16s rrna sequence analyses and phenotypic characterisations. the study was carried out on aestivating snails and two populations of h. pomatia were considered. the cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) cfu g -1, but the swedish h. pomatia appeared significantly less colonised, suggesting a higher sensitivity ... | 2007 | 17657348 |
| f3/contactin-related proteins in helix pomatia nervous tissue (hcrps): distribution and function in neurite growth and neurotransmitter release. | by using antibodies against mouse f3/contactin, we found immunologically related glycoproteins expressed in the nervous tissue of the snail helix pomatia. helix contactin-related proteins (hcrps) include different molecules ranging in size from 90 to 240 kd. clones isolated from a cdna expression library allowed us to demonstrate that these proteins are translated from a unique 6.3-kb mrna, suggesting that their heterogeneity depends on posttranslational processing. this is supported by the resu ... | 2008 | 17941055 |
| proteome analysis of metastatic colorectal cancer cells recognized by the lectin helix pomatia agglutinin (hpa). | the lectin from helix pomatia (hpa) binds to adenocarcinomas with a metastatic phenotype but the glycoconjugates of cancer cells that bind to the lectin have yet to be characterized in detail. we used a model of metastatic (ht29) and nonmetastatic (sw480) human colorectal cancer cells and a proteomic approach to identify hpa binding glycoproteins. cell membrane proteins purified by hpa affinity chromatography, were separated by 2-de and analyzed by ms. competitive inhibition experiments with n-a ... | 2007 | 17994629 |
| o-diphenol oxidase activity of molluscan hemocyanins. | diphenoloxidase activities of two molluscan hemocyanins, isolated from the marine snails rapana venosa and garden snails helix vulgaris were studied using o-diphenol and l-dopa as substrates. the dimers of h. vulgaris hc show both, diphenol (k(m)=2.86 mm and k(cat)=4.48) and l-dopa activity due to a more open active sites of the enzyme and better access of the substrates. the k(m) value of molluscan h. vulgaris hc is very close to those of helix pomatia and sepia officinalis hcs, but several tim ... | 2008 | 18162195 |
| pharmacokinetics and metabolism of benzophenone 2 in the rat. | twelve derivatives of benzophenone (bp1-bp12) are widely used as uv-screens to protect industrial products from light induced damage. there is growing public concern about industrially produced chemicals that might interfere with hormonal signalling pathways, thus having potential adverse effects on human health. the derivative 2,2',4,4'-tetrahydroxybenzophenone (bp2) which is used in cosmetic products and in packaging materials, was previously shown to be an estrogenic endocrine active chemical ... | 2008 | 18242814 |
| optimization of solid phase extraction clean up and validation of quantitative determination of corticosteroids in urine by liquid chromatography-tandem mass spectrometry. | a solid phase extraction (spe) method for extraction and clean up of 9 synthetic corticosteroids was optimized for quantification by reversed-phase high-performance liquid chromatography/negative electrospray ionisation mass spectrometry. clean up was accomplished using a mixed mode polymeric strong anion exchange spe column. the final method was validated according to eu regulations for determination of residues of veterinarian drugs in products of animal origin. initial results showed a large ... | 2008 | 18486661 |
| the presence and distribution of pituitary adenylate cyclase activating polypeptide and its receptor in the snail helix pomatia. | the aim of this study was to show the presence, distribution and function of the pituitary adenylate cyclase activating polypeptide (pacap) and its receptors in the cns and peripheral nervous system of the mollusk, helix pomatia. pacap-like and pituitary adenylate cyclase activating polypeptide receptor (pac1-r)-like immunoreactivity was abundant both in the cns and the peripheral nervous system of the snail. in addition several non-neuronal cells also revealed pacap-like immunoreactivity. in in ... | 2008 | 18590802 |
| measuring urinary testosterone levels of the great apes--problems with enzymatic hydrolysis using helix pomatia juice. | helix pomatia (hp) juice is a common enzymatic preparation for deconjugation of urinary steroids. it has been used in many published studies on urinary testosterone (t) in chimpanzees and bonobos, although the ability of hp juice to convert other urinary steroids into t has been reported for human urine. we developed a protocol for determination of reliable t levels in primate urine using liquid chromatography-mass spectrometry. t levels were determined in a set of human, bonobo and chimpanzee u ... | 2008 | 18639242 |
| the c-type lectin macrophage galactose-type lectin impedes migration of immature apcs. | dendritic cells (dcs) are the most potent apcs of the immune system that seed the peripheral tissues and lymphoid organs. in an immature state, dcs sample their surroundings for incoming pathogens. upon ag encounter, dcs mature and migrate to the lymph node to induce adaptive immune responses. the c-type macrophage galactose-type lectin (mgl), expressed in immature dcs, mediates binding to glycoproteins carrying galnac moieties. in the present study, we demonstrate that mgl ligands are present o ... | 2008 | 18713985 |
| characterization and role of helix contactin-related proteins in cultured helix pomatia neurons. | we report on the structural and functional properties of the helix contactin-related proteins (hcrps), a family of closely related glycoproteins previously identified in the nervous system of the land snail helix pomatia through antibodies against the mouse f3/contactin glycoprotein. we focus on hcrp1 and hcrp2, soluble fniii domains-containing proteins of 90 and 45 kd bearing consensus motifs for both n- and o-glycosylation. using the anti-hcrps serum, we find secreted hcrps in helix nervous ti ... | 2009 | 18756517 |
| quantification of 19-nortestosterone sulphate and boldenone sulphate in urine from male horses using liquid chromatography/tandem mass spectrometry. | following administration of the anabolic steroid 19-nortestosterone or its esters to the horse, a major urinary metabolite is 19-nortestosterone-17beta-sulphate. the detection of 19-nortestosterone in urine from untreated animals has led to it being considered a naturally occurring steroid in the male horse. recently, we have demonstrated that the majority of the 19-nortestosterone found in extracts of 'normal' urine from male horses arises as an artefact through decarboxylation of the 19-carbox ... | 2008 | 18777513 |
| phenotypic and genotypic characterisation of blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. | despite frequent reports on the presence of blastocystis hominis in human intestinal tract, its pathogenicity remains a matter of intense debate. these discrepancies may be due to the varying pathogenic potential or virulence of the isolates studied. the present study represents the first to investigate both phenotypic and genotypic characteristics of b. hominis obtained from symptomatic and asymptomatic individuals. symptomatic isolates had a significantly greater size range and lower growth ra ... | 2008 | 18795333 |
| experiments on the carbon dioxide transport by the blood of the edible snail (helix pomotial) the common crab (cancer pagurus l.) and the common lobster (homarus vulgaris m. e.). | | 1948 | 18874549 |
| expression of helix pomatia lectin binding glycoproteins in women with breast cancer in relationship to their blood group phenotypes. | aberrant glycosylation occurs in essentially all types of human cancers. a difference in glycopattern of proteins will result in a change of function of the proteins. the lectin from helix pomatia (hpa) recognizes n-acetylgalactosaminylated glycoproteins and very consistent results over the increased binding of hpa in tissue sections are associated with metastasis progression and poor patient prognosis in a range of human adenocarcinomas. the induced modification of protein function after change ... | 2009 | 18998722 |
| an enzyme cocktail for efficient protoplast formation in aspergillus niger. | novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. since it is no longer on the market, a new enzyme cocktail was defined to protoplast aspergillus niger. the cocktail consists of lysing enzymes from trichoderma harzianum, chitinase from streptomyces griseus and beta-glucuronidase from helix pomatia. | 2009 | 19041907 |
| specificity analysis of lectins and antibodies using remodeled glycoproteins. | due to their ability to bind specifically to certain carbohydrate sequences, lectins are a frequently used tool in cytology, histology, and glycan analysis but also offer new options for drug targeting and drug delivery systems. for these and other potential applications, it is necessary to be certain as to the carbohydrate structures interacting with the lectin. therefore, we used glycoproteins remodeled with glycosyltransferases and glycosidases for testing specificities of lectins from aleuri ... | 2009 | 19123999 |
| synthesis of sulfated glucosaminides for profiling substrate specificities of sulfatases and fungal beta-n-acetylhexosaminidases. | systematic sulfation: sulfated glycoconjugates are degraded either by desulfation followed by glycoside cleavage, or by glycoside cleavage followed by desulfation. to study these processes, here we report the synthesis of four regioisomerically sulfated p-nitrophenyl glucosaminides from the common precursor p-nitrophenyl n-acetyl-beta-d-glucosaminide. these substrates allowed the rapid analysis of the substrate preferences of a set of four sulfatases and 24 hexosaminidases.sulfated carbohydrates ... | 2009 | 19156788 |
| optimization and validation of a liquid chromatography tandem mass spectrometry (lc/msn) method for analysis of corticosteroids in bovine liver: evaluation of keyhole limpet beta-glucuronidase/sulfatase enzyme extract. | a liquid chromatography tandem mass spectrometry (lc/ms(n)) method for the determination of 12 corticosteroids in bovine liver has been optimized and validated in accordance with the european commission decision 2002/657/ec. a bovine liver sample was deconjugated with beta-glucuronidase/sulfatase enzyme, extracted with diethyl ether and further cleaned up with solid phase extraction (spe) before analysis with lc/ms(n). two different enzyme extracts (originating from helix pomatia and keyhole lim ... | 2009 | 19211313 |
| the immunological effects of electrolyzed reduced water on the echinostoma hortense infection in c57bl/6 mice. | electrolyzed reduced water (erw) is widely used for drinking by people in asia. the purpose of this study was to examine the immunological effect of erw on the immunity of animals by supplying erw to c57bl/6 mice infected with echinostoma hortense metacercariae. in the non-infected groups, interleukin (il)-4 (p < 0.001), il-5, il-10, il-1beta, tumor necrosis factor (tnf)-alpha and immunoglobulin (ig) a expression of the group fed erw (erw group) increased in small intestine compared with the nor ... | 2009 | 19252295 |
| acetylcholine inhibits nitric oxide (no) synthesis in the gastropod nervous system. | acetylcholine (ach) is one of the main signals regulating nitric oxide synthase (nos) expression and nitric oxide (no) biosynthesis in mammals. however, few comparative studies have been performed on the role of ach on nos activity in non-mammalian animals. we have therefore studied the cholinergic control of nos in the snail helix pomatia and compared the effects of ach on no synthesis in the enteric nervous system of the snail and rat. analyses by the nadph-diaphorase reaction, immunocytochemi ... | 2009 | 19255787 |
| identification and characterization of major proteins carrying abo blood group antigens in the human kidney. | it is generally admitted that abo(h) blood group antigens are linked to lipids and proteins. although glycolipids carrying abo antigens have been well characterized in human kidneys, glycoproteins carrying abo antigens are largely unknown, and their molecular properties remain to be elucidated. | 2009 | 19384157 |
| lectin mapping reveals stage-specific display of surface carbohydrates in in vitro and haemolymph-derived cells of the entomopathogenic fungus beauveria bassiana. | the entomopathogenic fungus beauveria bassiana and its insect host target represent a model system with which to examine host-pathogen interactions. carbohydrate epitopes on the surfaces of fungal cells play diverse roles in processes that include adhesion, non-self recognition and immune invasion with respect to invertebrate hosts. b. bassiana produces a number of distinct cell types that include aerial conidia, submerged conidia, blastospores and haemolymph-derived cells termed in vivo blastos ... | 2009 | 19608611 |
| tumor-specific protein human galectin-1 interacts with anticancer agents. | the present work shows a novel binding activity of the tumor specific lectin--recombinant human galectin-1 (hgal-1)--to three porphyrin compounds: (1) zn-porphyrin (zntpps); (2) mn-porphyrin and (3) au-porphyrin. these compounds are widely applied in the photodynamic therapy of cancer (pdt). our data indicate that hgal-1, similar to some plant lectins, a bacterial lectin from pseudomonas aeruginosa and an animal lectin from helix pomatia, possesses dual functions binding to both carbohydrate and ... | 2009 | 19823749 |
| decreasing prevalence of herpes simplex virus-2 antibodies in selected groups of women in sweden. | high estimates of herpes simplex virus-2 (hsv-2) seroprevalence have been reported for women who were pregnant in sweden in the 1980s, probably reflecting sexual risk-taking during the 1960s and 1970s. the aim of the present study was to evaluate the hsv-2 seroprevalence in pregnant women and in the female attendees at a clinic for sexually transmitted infections in sweden at the beginning of the 21st century and to compare the results with those of earlier swedish studies. sera were collected d ... | 2009 | 19997695 |
| e-/p-selectins and colon carcinoma metastasis: first in vivo evidence for their crucial role in a clinically relevant model of spontaneous metastasis formation in the lung. | interactions of endothelial selectins with tumour cell glycoconjugates have been shown to have a major role in tumour cell dissemination in previous experiments. however, experiments validating this observation were limited in value, as 'metastases' in these experiments were artificially induced by i.v. injection rather than developed spontaneously as in true metastases. | 2010 | 20010946 |
| enzymatic glycosylation, inhibitor design, and synthesis and formation of glyco-self assembled monolayers for simulation of recognition. | glycosyltransferases from the albumen gland of helix pomatia could be used in tandem mode for the chemoenzymatic synthesis of beta,1-3/beta,1-6-linked oligogalactans. by employing recombinant trans-sialidase of trypanosoma cruzi (tcts) the formation of a range of modified galbeta,1-3galnac derivatives could be terminally alpha,2-3 sialylated. biacore studies indicated the binding of these modified trisaccharides to myelin-associated glycoprotein (mag). using an eight-step synthetic route n-acyl- ... | 2010 | 20042251 |
| systematic error in gas chromatography-mass spectrometry-based quantification of hydrolyzed urinary steroids. | gas chromatography-mass spectrometry-based metabolite profiling can lead to an understanding of various disease mechanisms as well as to identifying new diagnostic biomarkers by comparing the metabolites related in quantification. however, the unexpected transformation of urinary steroids during enzymatic hydrolysis with helix pomatia could result in an underestimation or overestimation of their concentrations. a comparison of beta-glucuronidase extracted from escherichia coli revealed 18 conver ... | 2010 | 20086107 |
| survey of glycoantigens in cells from alpha1-3galactosyltransferase knockout pig using a lectin microarray. | glycoantigens represent major obstacles to successful xenotransplantation. even after the alpha1-3galactosyltransferase (galt) gene knockout (galt-ko) pigs were produced, non-gal antigens continue to be present. this study reports on lectin blot analyses for endothelial cells (ec) and fibroblasts from galt-ko pigs. | 2010 | 20149189 |
| lectin-binding sites on ejaculated stallion sperm during breeding and non-breeding periods. | stallion sperm from semen collected in southern italy during the breeding (june-july) and non-breeding (december-january) periods were analyzed by means of twelve lectins to evaluate the glycoconjugate pattern and to verify whether there are any seasonal differences in the glycosylation pattern of the sperm glycocalyx. the acrosomal cap showed reactivity for maackia amurensis (mal ii), sambucus nigra (sna), arachis hypogaea (pna), glycine max (sba), helix pomatia (hpa), canavalia ensiformis (con ... | 2010 | 20172596 |
| feasibility of capillary liquid chromatography/microchip atmospheric pressure photoionization mass spectrometry in analyzing anabolic steroids in urine samples. | we examined the feasibility of capillary liquid chromatography/microchip atmospheric pressure photoionization tandem mass spectrometry (caplc/microappi-ms/ms) for the analysis of anabolic steroids in human urine. the urine samples were pretreated by enzymatic hydrolysis (with beta-glucuronidase from helix pomatia), and the compounds were liquid-liquid extracted with diethyl ether. after separation the compounds were vaporized by microchip appi, photoionized by a 10 ev krypton discharge lamp, and ... | 2010 | 20209666 |
| determination of alkylresorcinol metabolites in human urine by gas chromatography-mass spectrometry. | alkylresorcinols (ars) are phenolic lipids present at high concentrations in the outer parts of rye and wheat kernels and have been proposed as biomarkers for intake of whole grain and bran products of these cereals. ar are absorbed in the small intestine and after hepatic metabolism two major metabolites, 3,5-dihydroxybenzoic acid (dhba) and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (dhppa), are excreted in urine either as such or as conjugates. urine samples from nine individuals were incubated ... | 2010 | 20223713 |
| mechanisms of achromatic vision in invertebrates and vertebrates: a comparative study. | intracellular recording in the retina of the snail, helix pomatia l., reveals the existence of two types of cell responsive to diffuse flashes of achromatic or monochromatic light: b-type cells, which respond with sustained depolarization that is sometimes accompanied by spikes, and d-type cells, which respond with sustained hyperpolarization. the peak of spectral sensitivity for both b- and d-cells falls in the 450-500 nm range and coincides with range of maximal sensitivity for the rhodopsin f ... | 2010 | 20480676 |
| a novel reductive transformation of oxazepam to nordiazepam observed during enzymatic hydrolysis. | beta-glucuronidase is an enzyme often employed to de-conjugate beta-glucuronides during urinary drug testing for benzodiazepines. it is commonly accepted that use of beta-glucuronidase is a preferred method of hydrolysis over acid-catalyzed hydrolysis, which is known to induce benzodiazepine degradation and transformation. literature to date, however, has not reported any cases of benzodiazepine transformation initiated by commercial beta-glucuronidase products. in this study, urine specimens co ... | 2010 | 20529458 |
| first trimester histiotrophe shows altered sialylation compared with secretory phase glycoconjugates in human endometrium. | histiotrophe is now recognized as being an important feature of early human pregnancy, providing nutrients and growth factors to the developing embryo. our aim was to examine the glycan composition of histiotrophe from first trimester decidua and to compare it with secretions present in endometrial glands from the late secretory phase of the menstrual cycle. | 2010 | 20569980 |
| profiling oestrogens and testosterone in human urine by stable isotope dilution/benchtop gas chromatography-mass spectrometry. | oestrogens, such as oestrone (e(1)), 17β-oestradiol (e(2)), oestriol (e(3)) and their biologically active metabolites 2-methoxyoestrone (2-meoe(1)), 2-hydroxyoestradiol (2-ohe(2)) 16-ketooestradiol (16-oe(2)), 16-epioestriol (16-epie(3)), as well as testosterone (t) play an important role in physiological and pathological developmental processes during human development. we therefore aimed at developing an isotope dilution/bench top gas chromatography-mass spectrometry (id/gc-ms) method, based o ... | 2010 | 20619283 |
| solid-phase synthesis of a pentavalent galnac-containing glycopeptide (tn antigen) representing the nephropathy-associated iga hinge region. | incomplete or aberrant glycosylation leading to tn antigen (galnacalpha1-ser/thr) expression on human glycoproteins is strongly associated with human pathological conditions, including tumors, certain autoimmune diseases, such as the idiopathic iga nephropathy, and may modulate immune homeostasis. in addition, the tn antigen is highly expressed by certain pathogens and plays a role in host-pathogen interactions. to enable experimental approaches to study interactions of the tn antigen with the i ... | 2010 | 20719305 |
| deconjugation kinetics of glucuronidated phase ii flavonoid metabolites by beta-glucuronidase from neutrophils. | flavonoids are inactivated by phase ii metabolism and occur in the body as glucuronides. mammalian beta-glucuronidase released from neutrophils at inflammatory sites may be able to deconjugate and thus activate flavonoid glucuronides. we have studied deconjugation kinetics and ph optimum for four sources of beta-glucuronidase (human neutrophil, human recombinant, myeloid plb-985 cells, helix pomatia) with five flavonoid glucuronides (quercetin-3-glucuronide, quercetin-3'-glucuronide, quercetin-4 ... | 2010 | 20814159 |
| expression and localization of mcsialec, a sialic acid-specific lectin in the marine bivalve manila clam, ruditapes philppinarum. | a novel sialic acid-specific lectin (mcsialec) was detected from an expressed sequenced tag (est) sequence from manila clam haemocytes infected with perkinsus olseni. the cdna of the lectin was cloned using gene-specific primers based on a previously determined est and characterized. the full-length cdna of mcsialec is 603 bp in length and encodes a polypeptide of 200 amino acids with a calculated molecular mass of 21.928 kda. sequence alignment and protein motif analyses showed that mcsialec sh ... | 2010 | 21039608 |
| effects of an oxidative agent and lectins on the binding inhibition of recombinant hepatitis a virus proteins to oyster digestive tissues. | while the exact mechanism of hepatitis a virus (hav) accumulation remains unclear, it has been demonstrated that viruses related to shellfish-borne gastroenteritis can bind to carbohydrates of oyster tissues. we investigated carbohydrate-binding sites to determine if they were related to the binding of hav to carbohydrate moieties on oyster digestive tissues (dts) using recombinant hav proteins (rhavps). in addition, we evaluated lectins to determine if they influenced the inhibition of binding ... | 2011 | 21219781 |
| identification of n-acetylgalactosamine in carbohydrates of xenopus laevis testis. | identification of glycans in amphibian testis has shown the existence of n-acetylgalactosamine (galnac)-containing carbohydrates. labeling of the sperm acrosome with galnac-binding lectins has allowed the identification of galnac-containing glycans in this organelle. futhermore, this specific labeling of the acrosome has allowed the study of acrosomal biogenesis by lectin histochemistry. however, the testis of xenopus laevis has never been analyzed by lectin histochemistry to locate galnac-conta ... | 2010 | 21235011 |
| shaping mechanisms of metal specificity in a family of metazoan metallothioneins: evolutionary differentiation of mollusc metallothioneins. | the degree of metal binding specificity in metalloproteins such as metallothioneins (mts) can be crucial for their functional accuracy. unlike most other animal species, pulmonate molluscs possess homometallic mt isoforms loaded with cu(+) or cd(2+). they have, so far, been obtained as native metal-mt complexes from snail tissues, where they are involved in the metabolism of the metal ion species bound to the respective isoform. however, it has not as yet been discerned if their specific metal o ... | 2011 | 21255385 |
| chemoenzymatic synthesis of diverse thiohydroximates from glucosinolate-utilizing enzymes from helix pomatia and caldicellulosiruptor saccharolyticus. | thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. we hypothesized that sequential action of two recombinant enzymes, a sulfatase from helix pomatia and a ß-o-glucosidase from caldicellulosiruptor saccharolyticus, on glucosinolates would allow synthesis of thiohydroximates from a structurally broad arra ... | 2011 | 21267762 |
| reduction of temazepam to diazepam and lorazepam to delorazepam during enzymatic hydrolysis. | it has been previously reported that treatment of urinary oxazepam by commercial ß-glucuronidase enzyme preparations, from escherichia coli, helix pomatia and patella vulgata, results in production of nordiazepam (desmethyldiazepam) artefact. in this study, we report that this unusual reductive transformation also occurs in other benzodiazepines with a hydroxyl group at the c3 position such as temazepam and lorazepam. as determined by liquid chromatography-mass spectrometry analysis, all three e ... | 2011 | 21298417 |
| identification, cloning, and characterization of two n-acetylgalactosamine-binding lectins from the albumen gland of helix pomatia. | helix pomatia agglutinin (hpa), the lectin from the albumen gland of the roman snail, has been used in histochemical studies relating glycosylation changes to the metastatic potential of solid tumors. to facilitate the use of hpa in a clinical (diagnostic) setting, detailed analysis of the lectin, including cloning and recombinant production of hpa, is required. a combination of isoelectric focusing, amino acid sequence analysis, and cloning revealed two polypeptides in native hpa preparations ( ... | 2011 | 21372134 |
| analysis of 4-aminobiphenyl in smoker's and nonsmoker's urine by tandem mass spectrometry. | the aromatic amine 4-aminobiphenyl (4-abp) is present in tobacco smoke. in humans, it is also a known bladder carcinogen. we describe here a method for the quantification of total 4-abp in urine using capillary gas chromatography/tandem mass spectrometry, with an effective detection limit in urine samples of approximately 0.87 pg/ml. we also examined the efficiency of chemical or enzymatic hydrolysis of urinary aromatic amine metabolites. although we found acidic or basic hydrolysis effective, w ... | 2011 | 21438718 |
| bacterial adherence in otitis media: determination of n-acetylgalactosamine (galnac) residues in the submucosal glands and surface epithelium of the normal and diseased eustachian tube. | acute otitis media (aom) is the most common childhood infection caused by bacteria. the pathogenesis of aom implicates initial adherence of a pathogen to the nasopharyngeal epithelium, which is followed by bacterial colonization of the middle ear cavity through the eustachian tube. n-acetylgalactosamine (galnac) is an important constituent of mucins and galnac containing sugar residues seem to be essential for initial adherence of respiratory bacteria to the surface of epithelial cells. | 2011 | 21440051 |
| effect of tear hyperosmolarity and signs of clinical ocular surface pathology upon conjunctival goblet cell function in the human ocular surface. | purpose: to investigate the effect of tear hyperosmolarity and signs of clinical ocular surface pathology upon conjunctival goblet cell population. methods: 111 participants were assessed using tear osmolarity (to) measurements and a comprehensive selection of clinical ophthalmic tests. the resultant clinical database was assessed for evidence of patterns of composite increasing pathology. the total, filled, and empty goblet cell numbers were measured: total number of goblet cells as per cytoker ... | 2011 | 21519030 |
| phenoloxidase activity of intact and chemically modified functional unit rvh1-a from molluscan rapana venosa hemocyanin. | o-diphenol oxidase activities (o-dipo) of chemically modified functional unit rvh1-a of molluscan hemocyanin rapana venosa were studied using l-dopa and dopamine as substrates. with l-dopa as substrate the native fu rvh1-a did not show any o-dipo activity. therefore the native fu rvh1-a was converted to enzymatic active form, after treatment with sds, trypsin, urea and different values of ph when its o-dipo activity was studied. the highest artificial induction of o-dipo activity was observed af ... | 2011 | 21536147 |
| nm23 expression versus helix-pomatia lectin-binding in human breast-cancer metastases. | the relationship between nm23 expression, hpa staining, and other clinicopathological variables, i.e., axillary (ax) and internal mammary lymph node (imn) metastases was assessed, and their prognostic values were analyzed. positive hpa staining was found to be significantly correlated with tumor size, and with ax and imn metastases, whereas negative nm23 expression was significantly correlated with ax and imn metastases. a univariate study revealed that overall survival was correlated significan ... | 1994 | 21567061 |
| investigation of the persistence of nitroxynil residues in milk from lactating dairy cows by ultra performance liquid chromatography tandem mass spectrometry. | nitroxynil is an anthelmintic used in the treatment of liver fluke. in this study, six dairy cows were treated during lactation with trodax, a 34% solution containing nitroxynil as its n-ethylglucamine salt, indicated for the treatment of fascioliasis in cattle and sheep. samples were collected twice daily for 16 days and later at weekly intervals up to 58 days post-treatment. nitroxynil residues were extracted from milk samples using acetonitrile; magnesium sulfate and sodium chloride were adde ... | 2011 | 21657788 |
| bioassay based screening of steroid derivatives in animal feed and supplements. | receptor binding transcription activation bioassays are valuable tools for the screening of steroid hormones in animal feed and supplements. however, steroid derivatives often lack affinity for their cognate receptor and do not show any direct hormonal activity by themselves. these compounds are thus not detected by these kinds of bioassays and need a bioactivation step in order to become active, both in vivo and in vitro. in this study a comparison was made between different in vitro activation ... | 2011 | 21742131 |
| helix pomatia agglutinin binding glycoproteins in thyroid tumors. | binding of the lectin helix pomatia agglutinin (hpa) has been shown to be associated with poor prognosis in many human cancers, but not in thyroid cancer. the aims of the present study were to assess alteration in cellular glycosylation, detected by hpa binding, in thyroid tumors; to determine if such altered glycosylation carries any prognostic significance; and to analyze qualitative and quantitative differences of hpa-binding glycoproteins in various thyroid tumors. | 2011 | 21845460 |
| possible evidence of contamination by catechins in deconjugation enzymes from helix pomatia and abalone entrails. | β-glucuronidase and sulfatase are the major deconjugating enzymes used in the cleavage of the glucuronate and sulfate moieties, respectively, from certain conjugated food factors including polyphenols. in the present study, we found that compounds having the same molecular weights as catechins were present in helix pomatia- and/or abalone entrails-derived β-glucuronidase and sulfatase by liquid chromatography tandem mass spectrometry (lc-ms/ms) with multiple reaction monitoring methods. on the o ... | 2011 | 21821947 |
| Hepatoprotective activity of the edible snail (Bellamia bengalensis) flesh extract in carbon tetrachloride induced hepatotoxicity in rats. | In the folk-traditional medicine, snails were used to purify blood, boost immune system, prevent conjunctivitis and to treat liver problems. | 2011 | 21945824 |
| effect of the gc-derived macrophage-activating factor precursor (pregcmaf) on phagocytic activation of mouse peritoneal macrophages. | the 1f1f subtype of the gc protein (gc(1f1f) protein) was converted into gc-derived macrophage-activating factor (gcmaf) by enzymatic processing in the presence of β-galactosidase of an activated b-cell and sialidase of a t-cell. we hypothesized that pregc(1f1f)maf, the only gc(1f1f) protein lacking galactose, can be converted to gcmaf in vivo because sialic acid is cleaved by residual sialidase. hence, we investigated the effect of pregc(1f1f)maf on the phagocytic activation of mouse peritoneal ... | 2011 | 21873164 |
| differences in predatory pressure on terrestrial snails by birds and mammals. | the evolution of shell polymorphism in terrestrial snails is a classic textbook example of the effect of natural selection in which avian and mammalian predation represents an important selective force on gene frequency. however, many questions about predation remain unclear, especially in the case of mammals. we collected 2000 specimens from eight terrestrial gastropod species to investigate the predation pressure exerted by birds and mice on snails. we found evidence of avian and mammalian pre ... | 2011 | 21857115 |
| The overlooked difference between human endogenous and recombinant erythropoietins and its implication for sports drug testing and pharmaceutical drug design. | Sequential deglycosylation by exoglycosidase treatment (Reagent Array Analysis Method, RAAM) and subsequent sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a profound structural difference between human endogenous and recombinant erythropoietins. While both proteins behaved similarly upon digestion with Arthrobacter ureafaciens a-sialidase and Steptococcus pneumoniae ß-D-galactosidase, the action of N-acetyl-ß-D-glucosaminidase from Steptococcus pneumoniae was partl ... | 2011 | 22140023 |
| MDR-1-overexpression in HT 29 colon cancer cells grown in SCID mice. | The multidrug-resistance 1 (MDR-1) P-glycoprotein (Pgp) is a transmembrane transporter system, which actively pumps cytotoxic drugs out of the cell. MDR-1 acquired in vitro differs from MDR-1 acquired in vivo, but has important consequences on the cellular phenotype and metastatic behavior. Here we report that the human colonic cancer cell line HT29 (MDR-1 negative) is more malignant than its MDR-1 overexpressing variant (HT29 MDR-1 positive). HT29 MDR-1 negative cells produce undifferentiated s ... | 2011 | 22154301 |
| study of the pest community of velvetleaf (abutilon theophrasti medic.). | velvetleaf (abutilon theophrasti medicus 1787) is one of the most economically threatening weed plant in hungary. researching biological control against it, and identifying a possible and effective biocontrol agent is an important challenge, as chemical control is difficult and expensive, and there is an increasing claim to practice slight plant protection. entomological studies were made in several parts of the world, for evaluating the species, occuring in velvetleaf, but none of these kind of ... | 2011 | 22696962 |
| purification of a -1,4-glucan hydrolase (cellulase) from the snail, helix pomatia. | | 2016 | 4714910 |
| study of neurosecretory cells of helix pomatia by intracellular dye injection. | | 2014 | 4104442 |
| [effect of ionizing radiation on the functional state of snail neurons. phospholipid composition and levels of free fatty acids]. | a study was made of the effect of ionizing radiation on the phospholipid composition and the level of free fatty acids in nervous ganglia of edible snails (helix pomatia). ionizing radiation was shown to decrease the total content of phospholipids and to change certain fractions and accumulation of lysoforms. the increase in the free fatty acid content indicated an activation of endogenous phospholipase hydrolysis. model experiments on treatment of nervous ganglia with phospholipase a2 demonstra ... | 2010 | 3628736 |
| [spectrofluorometric and cytochemical research on biogenic amines in the cerebral ganglion of helix pomatia]. | | 2014 | 4143424 |
| 17 alpha,20 beta-dihydroxy-4-pregnen-3-one 20-sulphate: a major new metabolite of the teleost oocyte maturation-inducing steroid. | this paper describes the discovery of 17 alpha,20 beta-dihydroxy-4-pregnen-3-one 20-sulphate (17,20 beta-p-sulphate) in urine of male and female plaice pleuronectes platessa, in female atlantic salmon salmo salar and in female dover sole solea solea. 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-p) induces oocyte final maturation in teleosts and, whereas levels of the free steroid in maturing/ovulating female plaice are generally less than 1 ng ml-1 and poorly associated with the stage ... | 2006 | 1563623 |
| purification of cadmium-binding proteins from related species of terrestrial helicidae (gastropoda, mollusca): a comparative study. | three species of terrestrial helicidae (helix pomatia, cepaea hortensis and arianta arbustorum) were fed cadmium-rich diet in the laboratory. the snails accumulated high amounts of the metal in their hepatopancreas. most cadmium and some zinc were found, after centrifugation, in the soluble fractions from which a cadmium-binding protein was isolated for each species by ion exchange and gel chromatography. the proteins contained different amounts of cadmium, but little or no zinc, and showed high ... | 2009 | 2725484 |
| [effects of fmrfamide on the activity of command neurons in defensive behavior of fed and fasting snails, helix pomatia]. | fmrfamide (phe-met-arg-phe-nh2) micropneumophoresis changed bimodally the activity of lpa2, lpa3, ppa2 and ppa3 neurones in fasting and fed helix pomatia. in fasting creatures peptide application elicited hyperpolarization and decreased the neuronal membrane excitability and responses to tactile stimulation. in fed snails peptide application caused depolarization, decreased membrane resistance and increased the neuronal membrane excitability and responses to tactile stimulation. neurophysiologic ... | 2012 | 3382722 |
| primary structure of the low-molecular-weight carbohydrate chains of helix pomatia alpha-hemocyanin. xylose as a constituent of n-linked oligosaccharides in an animal glycoprotein. | alpha-hemocyanin of helix pomatia is a copper-containing glycoprotein which serves as an oxygen carrier in the hemolymph. its carbohydrate moiety has as constituents fucose, xylose, 3-o-methylgalactose, mannose, galactose, n-acetylgalactosamine, and n-acetyl-glucosamine residues. alkaline borhydride did not split off any carbohydrate material, suggesting the absence of o-glycosidic chains. the n-glycosidic carbohydrate chains of this glycoprotein were liberated by hydrazinolysis of a pronase dig ... | 2014 | 4055767 |