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increased multiplication of dengue virus in mouse peritoneal macrophage cultures by treatment with extracts of ascaris-parascaris parasites.methylcellulose-elicited peritoneal macrophages from balb/c mice were cultivated in vitro and inoculated with dengue virus (dv). at intervals thereafter portions of the culture fluids were taken and titrated for viral infectivity. extracts from ascaris suum and parascaris equorum, either crude or sephadex g-100 fractionated, were examined for effects on the multiplication of dv. the macrophage cultures treated with the above substances produced larger amounts of dv compared with untreated contro ...19853894890
increased in vitro airway responsiveness in sheep following repeated exposure to antigen in vivo.we have studied the effect of repeated in vivo antigen exposure on in vitro airway responsiveness in sensitized sheep. fourteen sheep underwent five biweekly exposures to aerosolized ascaris suum antigen or saline. following this exposure regimen, the animals were killed and tracheal smooth muscle and lung parenchymal strips were prepared for in vitro studies of isometric contraction in response to histamine, methacholine, prostaglandin f2 alpha, and a thromboxane a2 analogue. no alteration in t ...19853908441
influence of chronic antigen exposure on the metabolism of pgh2 by microsomal preparations of airway and parenchymal tissues in the sheep.the effects of repeated antigen exposure on the synthesis of mediators by lung tissues are not well understood. to investigate the influence of antigen challenge on the synthesis of prostaglandins by central airway and peripheral lung tissues, fourteen sensitive sheep underwent biweekly exposure to aerosolized ascaris suum antigen (7) or saline (7). following the fifth exposure, microsomal and high speed supernatant fractions were prepared from trachealis muscle and lung parenchyma. synthesis of ...19853911292
activity of rapidly-adapting receptors to histamine and antigen challenge before and after sodium cromoglycate.sodium cromoglycate (scg) blocks histamine release from sensitized mast cells challenged by antigen in vitro. yet, not all the observed effects of scg in vivo can be explained by this mechanism alone. rapidly-adapting or "irritant" receptors (rar) are thought to mediate reflex bronchoconstriction. others have proposed that scg may desensitize these receptors to histamine. we administered aerosols of histamine (100 micrograms/ml given for 3 min) before and after scg (20 mg/ml aerosol for 5 min) t ...19853923960
production of early and late pulmonary responses with inhaled leukotriene d4 in allergic sheep.some allergic sheep respond to inhalation of ascaris suum antigen with both immediate and late increases in airflow resistance (late response). the mechanism of the late response is unknown but recent evidence suggests that the initial generation of slow-reacting substance of anaphylaxis (srs-a) immediately after antigen challenge is a necessary pre-requisite for the physiologic expression of this late response. based on this evidence we hypothesized that airway challenge with leukotriene d4 (lt ...19853925500
ascaris suum: localization by immunochemical and fluorescent probes of host proteases and parasite proteinase inhibitors in cross-sections.cross-sections of muscle, intestine, and genital tract fluoresced in defined locations when live ascaris suum adults were incubated in medium containing chymotrypsin liganded with fluorescein-5-isothiocyanate. this suggests that the protease, or portions of it, are assimilated by a. suum. a. suum chymotrypsin/elastase isoinhibitors were found in muscle sarcolemma, eggs, sperm, and intestine, and host chymotrypsin was localized in the same regions of these tissues by immunofluorescence and immuno ...19853928399
[digestibility of nutrients in a feed mixture in certain swine helminthiases].the average digestibility coefficients of feed mixture nutrients in pigs infected medium severely to severely by the helminths ascaris suum and oesophagostomum dentatum were lower in comparison with the post-dehelminthization condition (dry matter by 3.1%, crude protein by 6.7%, fat by 8.9%, nitrogen-free extracts and fibre by 2.2%, organic matter by 3.1%). the content of total digestible nutrients made 679 g in infected pigs and 708 g in dehelminthized pigs. in the period following the experime ...19853931335
comparative purification and characterization of invertebrate muscle glycogen synthase from the porcine parasite ascaris suum.glycogen synthase has been purified from the obliquely striated muscle of the swine parasite ascaris suum. the muscle contains a concentration of glycogen synthase and glycogen which is 20-fold and 15-fold, respectively, greater than rabbit skeletal muscle. the enzyme could not be solubilized with salivary amylase, but partial solubilization was achieved by activation of endogenous phosphorylase. the enzyme was purified to 85-90% homogeneity (specific activity = 4.3 units/mg) by deae-cellulose, ...19853934170
ascaris suum: regulation of myosin light chain phosphorylation from adult skeletal muscle.the regulatory myosin light chain in ascaris suum muscle was isolated in a phospho and dephosphoform. in freshly dissected tissue or in control muscle segments perfused with saline, approximately 48% of the total myosin light chain pool was phosphorylated. perfusion with acetylcholine caused a dose dependent increase in muscle contraction but no change in myosin light chain phosphorylation. in contrast, perfusion with gamma-aminobutyric acid resulted in a dose dependent relaxation of a. suum mus ...19863943587
intestinal basement membrane of ascaris suum. molecular organization and properties of the collagen molecules.the collagenous components of ascaris suum intestinal basement membrane were isolated by extraction with 0.1 m tris-hc1, 0.5 m nac1, 0.5% 2-mercaptoethanol, ph 8.3, and sephacryl s-300 gel filtration. rotary-shadowing electron microscopy showed that the collagenous components occur as monomers and dimers with mean contour lengths of 469 +/- 21 and 918 +/- 24 nm, respectively. the molecules each contain a globular domain, with that of the dimer being slightly larger than that of the monomer. elec ...19863957913
screen for anthelmintics, using larvae of ascaris suum.a multiwell culture system was used to assay the effects of 12 known anthelmintic compounds on ascaris suum larval development from 2nd-stage (l2; hatched from eggs) to early 3rd-stage (l3) and from in vivo-derived late l3 to early 4th-stage (l4). larval survival, development, and motility were monitored for drug effects. development of l2 to l3 was sensitive to thiabendazole, albendazole (abz), abz/sulfoxide, abz/sulfone (so), mebendazole, l-tetramisole, d-tetramisole, piperazine, or closantel ...19863963590
influence of an experimental infection of ascaris suum on performance of pigs.thirty-two pigs (average 26.6 kg live weight) were individually housed and fed to study the effect of an infection of ascaris suum (either 0, 600, 6,000 or 60,000 a. suum eggs/pig) on performance of growing-finishing pigs. increasing the level of a. suum infection produced linear (p less than .07) and quadratic (p less than .09) effects on final weight, weight gain and average daily gain. feed to gain ratio and number of a. suum worms recovered from the intestines of pigs at slaughter increased ...19853972742
steroidal enhancement of growth in parasitic larvae of ascaris suum: validation of a bioassay.third-stage larvae of the swine parasitic roundworm, ascaris suum, were grown in stationary multiwell cultures in the presence of synthetic steroid hormones or the hplc fractions of a steroid extract of adult female reproductive tracts to determine the subsequent effect of these compounds on length of 4th-stage larvae. log doses of ecdysone, 20-hydroxyecdysone, progesterone, and makisterone standards, from 50 pg/ml to 500 ng/ml, were incubated with larvae for 1 day in rpmi-1640 supplemented with ...19853973553
purification and characterization of the 2-methyl branched-chain acyl-coa dehydrogenase, an enzyme involved in nadh-dependent enoyl-coa reduction in anaerobic mitochondria of the nematode, ascaris suum.the 2-methyl branched-chain acyl-coa dehydrogenase was purified to homogeneity from mitochondria of the parasitic nematode, ascaris suum. the native molecular weight of the enzyme was estimated to be 170,000 by gel filtration. the enzyme migrated as a single protein band with mr = 42,500 during sodium dodecyl sulfate-polyacrylamide gel electrophoresis suggesting that the enzyme is a tetramer composed of identical subunits. the enzyme exhibited absorbance maxima at 272, 375, and 452 with a ratio ...19853988734
the effect of continuous in-feed medication with thiophanate on ascaris suum and oesophagostomum spp. egg output in young pigs.the full potential of anthelmintics now available for single dose treatment is not achieved because the devising system for worm control in piglets/weaners is not efficiently applicable in practice. therefore an in-feed medication programme for growing young pigs, allowing only one feed lot to be handled by the farmer, was tested in two studies. study a feed containing 0.0225% thiophanate was continuously fed almost ad lib. to piglets from birth right up to about 25 kg body weight when ready for ...19854000918
taenia hydatigena: a cause of persistent liver condemnations in lambs.a problem of high levels of liver condemnations which had affected bought-in lambs fattened on a north yorkshire farm for several years was investigated. because the farmer spread pig slurry on the land, migrating ascaris suum larvae were initially suspected as a cause but the investigation eliminated this possibility. migrating taenia hydatigena larvae were found in tissue sections and isolated from livers. a combination of parasitological and serological evidence using oncospheral antigen in a ...19854002589
immunization of pigs against ascaris suum by sequential experimental infections terminated with fenbendazole during larval migration.three experimental infections of weaning pigs with 2000 embryonated ascaris suum eggs each, 11 days apart, followed each time by fenbendazole treatment, produced a significant host response when compared with similar infected or uninfected control pigs as assessed by response to a subsequent challenge with 100 embryonated a. suum eggs. the response elicited from pigs treated with fenbendazole on either 2, 3, and 4 days or, 6, 7, and 8 days after each experimental infection was expressed as a red ...19854002602
penetration of ovicidal fungi through altered egg-shells of ascaris suum.ovicidal activity was studied in microscopic fungi penetrating into and destroying eggs of geohelminths surviving in the soil. the character of penetration was compared in eggs obtained mechanically with those obtained by means of a chemical process. a total of 13 strains of ovicidal fungi of 4 species were studied. the shells of eggs obtained by the chemical process were so altered that under the same conditions they were attacked and penetrated by the fungi much more than the eggs obtained by ...19854007704
induction and down-regulation of conjunctival type-i hypersensitivity reactions in guinea pigs sensitized topically with fluoresceinyl ovalbumin.type-i hypersensitivity reactions were induced in guinea pigs by repeated topical/conjunctival application of fluoresceinyl ovalbumin (fl-oa). the ocular reactivity in early responding animals was maximal between 16 and 25 days and decreased exponentially thereafter. desensitized eyes responded minimally to compound 48/80 but maximally to histamine. unilateral sensitization and challenge with fl-oa produced desensitization of the immunized eye, but the reactivity of the contralateral eye persist ...19854011127
late pulmonary response in guinea pigs after ascaris challenge.respiratory resistance of guinea pigs immunized with ascaris suum was measured over 6 hr after aerosol challenge of the allergen by use of the oscillation method. these animals showed a biphasic pulmonary response to the aerosol challenge with ascaris suum. histological analysis of these animals revealed a narrowing of bronchial lumen, peribronchial edema and neutrophil-rich mucous in bronchial lumen at the late pulmonary response.19854024080
diethylpyrocarbonate inactivation of nad-malic enzyme from ascaris suum.treatment with diethylpyrocarbonate results in a first-order loss of the malate oxidative decarboxylase activity of nad-malic enzyme. first-order plots are biphasic, with about 40-50% activity loss in the first phase. the inactivation process is not saturable, and the second-order rate constant is 4.7 m-1 s-1. malate (250 mm) provides complete protection against inactivation (as measured by a decrease in the inactivation rate), and less malate is required with mg2+ present. partial protection (5 ...19854026323
ascaris suum: role of ecdysteroids in molting.three studies were conducted to examine the function of ecdysteroids in the development of parasitic nematodes. ecdysone and 20-hydroxyecdysone were extracted, separated chromatographically, and measured in the reproductive tracts of adult female ascaris suum. perienteric fluid and the body wall did not contain measurable levels of these steroids. levels of 20-hydroxyecdysone were correlated with the third and fourth molts of larvae grown in vitro from the third stage. in a bioassay, addition of ...19854029350
ascaris suum: protective immunity in pigs immunized with products from eggs and larvae.parasite products were collected at three distinct phases of development of ascaris suum, and their immunogenicity was determined after injection into rabbits and pigs. products were derived from (1) the hatching fluid of infective eggs; (2) the conditioned medium of 2nd-stage larvae that developed to 3rd stage in vitro in defined medium; and (3) the conditioned medium of 3rd-stage larvae that developed to 4th stage in vitro in defined medium. protein profiles from these three preparations, sepa ...19854029352
growth and form of the fourth-stage larvae and early adults of ascaris suum goeze, 1782 (nematoda: ascaridoidea) developing in pigs.the fourth-stage larvae and adults of ascaris suum were studied as they developed in the pig intestine from 11 to 22 days after infection. this is a period of rapid growth, in which both sexes increase approximately ten-fold in body length. further examination of females showed the length increase to be positively allometric with respect to body diameter. the genital primordium in early fourth stage is at the same relative position along the length of the body as it is in the newly hatched larva ...19854031452
parallel induction of ige-mediated ascaris antigen airway responses and increased carbachol airway reactivity in rhesus monkeys by infection with ascaris suum.in continuation of studies of four rhesus monkeys infected with ascaris suum (j lab clin med 101:864-872, 1983), we report the duration of ige-mediated skin reactivity and airway responses to ascaris antigen from 15 weeks to longer than 2.8 years. a second group of four rhesus monkeys was infected with a. suum ova after determination of the threshold of airway reactivity to carbachol. evaluation of these animals confirmed the initial results of the first group of animals studied in relation to i ...19854031630
enzymes regulating glucosamine 6-phosphate synthesis in the zygote of ascaris suum. 19854040895
aminopeptidase inhibitor from ascaris suum.the correlation of aminopeptidase activity in the homogenate and perienteric fluid from ascaris suum to the concentration of the studied fluids was assessed. the obtained non-linear correlations were interpreted as a result of the presence of the inhibitor. in further experiments, it was demonstrated that aminopeptidase inhibitor is present in the homogenate and perienteric fluid from a. suum. the effect of this inhibitor was studied using different substrates. the effects of the inhibitor on am ...19854043864
effects of leukotriene d4 on mucociliary and respiratory function in allergic and nonallergic sheep.we determined the effect of aerosol challenge with leukotriene d4 (ltd4) on specific lung resistance (srl) and tracheal mucous velocity (tmv) in conscious sheep with (allergic) and without (nonallergic) ascaris suum hypersensitivity. in allergic sheep ltd4 in concentrations of 50, 100, and 150 micrograms/ml produced dose-dependent increases in mean srl by 44 (p = ns), 154 (p less than 0.05), and 233% (p less than 0.05), respectively. the increase in srl produced by 150 micrograms/ml ltd4 was pre ...19854066571
preliminary studies on the immunity to ascaris suum in pigs. 19714102499
origin and possible utilization of small dense granules in oocytes of ascaris suum. 19724115175
[evaluation of antigens from various developmental stages of ascaris suum]. 19744135385
ultraviolet light absorption and fluorescence properties of an allergen extracted from ascaris suum. 19744139124
movement of cholesterol and beta-sitosterol across the intestine of ascaris suum. 19744156258
movement of triglycerides and monoglycerides across the intestine of ascaris suum. 19744156259
[experimental studies on anthelmintics. (23). biochemical and pharmacological effects of 1-[2-(dodecyloxy) ethyl] pyrrolidine hydrochloride (dpe) on ascaris suum]. 19744156925
chemotactic response of polymorphonuclear leukocytes to trichinella spiralis and ascaris suum extracts. 19654158486
passive immunity to ascaris suum transferred in colostrum from sows to their offspring. 19654159283
passive immunity to migrating ascaris suum transmitted by parenterally administered immune serum or immune globulins. 19654159414
electron microscope observations on the cuticle and submicroscopic binding of antibody in ascaris suum larvae. 19674163376
fine structure of leukocytes adhering to the cuticle of ascaris suum larvae. i. pyroninophils. 19694179172
ascaris suum and toxocara canis: radioautographic detection of dna in feulgen-negative muscle nuclei. 19704192595
polymorphism of malate dehydrogenase in ascaris suum. 19704193184
isolation and characterization of a non-specific esterase enzyme of ascaris suum perienteric fluid. 19734202818
disc electrophoresis of ascaris suum female reproductive system esterase enzymes. 19734202819
characterisation of an allergen extracted from ascaris suum. determination of the molecular weight, isoelectric point, amino acid and carbohydrate content of the native allergen. 19734203356
effect of antiserum on maltase from intestinal microvillous membranes of ascaris suum. 19744205468
[comparative study of antigen extracts from ascaris suum, ascaris ovis and ascaris lumbricoides]. 19734209853
[comparative antigenic analysis of ascaris suum, ascaris lumbricoides, toxocara mystax and ascaridia galli by gel double diffusion and immunoelectrophoresis (author's transl)]. 19744218110
[adenosine triphosphatase activity in muscle tissue mitochondria of the ascarid (ascaris suum goeze, 1758)]. 19684239520
ascaris suum infection in calves. 3. pathology.gross changes in the lungs of ascaris suum- infected calves consisted of atelectasis and hemorrhagic foci, edema and emphysema, frequently with bullae. prominent microscopic lung lesions were edema and emphysema of the interlobular septa with large numbers of eosinophils within and around lymphatics, peribronchiolar lymphoid nodules and parasitic granulomas. many of the microscopic features were consistent with those found in atypical interstitial pneumonia. changes in the alveoli were atelectas ...19704248444
[influence of organo-stannato compounds on the development and viability of ascaris suum eggs]. 19714255757
nematode metabolism: the adenosine triphosphatase of ascaris suum muscle. 19724264328
reinfection of yearling calves with ascaris suum.naturally occuring outbreaks of ascaris suum infection in calves have usually beeen found in animals nine to 12 months of age. the circumstances surrounding these outbreaks suggest that yearling calves are either particularly susceptible to a primary exposure to a. suum or react strongly to a. summ after sensitization early in life to this or some related ascarid. to determine the effect of reinfection with a. suum nine to 12 months after varying levels of exposure to this nematode, six calves w ...19734265549
[use of antigenic extracts of ascaris suum for the diagnosis of filariasis by means of double diffusion. value compared with onchocerca volvulus and dipetalonema viteae antigens]. 19724267505
the ultrastructural localization of nonspecific phosphatases in the intestinal epithelium of ascaris suum. 19704317318
biochemical observations of ascaris suum lung-stage larvae. 19684319378
non-specific phosphomonoesterases of ascaris suum. i. effect of inhibitors, activators, and chelators. 19704323162
isolation and characterisation of a mast cell degranulating substance from ascaris suum. 19724335029
fine structure of the coelomocyte of adult ascaris suum. 19724345109
initial steps in the de novo pyrimidine biosynthesis in ascaris suum. 19734357382
immunology of ascaris suum infection. i. production of reaginic antibodies to worm components in rats. 19744362125
some properties of ascaris suum allergen a. 19744370597
ascaris suum infection in calves. i. clinical signs.clinical signs consistent with those of atypical interstitial pneumonia (aip) were induced in calves sensitized with infective ascaris suum eggs at seven to 20 weeks of age and challenged at three-week intervals one or more times. these signs usually appeared on the sixth or seventh day postinfection and reached maximum severity between the tenth and 13th days following infection. prominent signs were: dyspnea, often with expiratory grunt, coughing, mouth breathing and emphysema as well as incre ...19704394225
ascaris suum infection in calves. ii. circulating and marrow eosinophil responses.an increment in the number of circulating eosinophils occurred between the 11th and 14th days after infection with ascaris suum and this increase was generally greater after a challenge infection. continual infection with small numbers of a. suum eggs over prolonged periods resulted in circulating eosinophil levels which fluctuated and were dose-dependent. the per cent marrow eosinophils always increased after a primary infection and a greater increase usually followed a challenge infection. the ...19704394226
conversion of the alpha-hydroxy and alpha-keto analogues of methionine to methionine by cell-free extracts of adult female ascaris suum. 19714398138
studies on enzymes from parasitic helminths. i. purification and physical properties of malic enzyme from the muscle tissue of ascaris suum. 19724402077
the glutamic acid dehydrogenase of adult female ascaris suum. 19724402878
ascaris suum pneumonia in cattle. 19744412090
characterization of an allergen isolated from ascaris suum. ii. the conformation of allergen a studied by circular dichroism and infra-red spectroscopy. 19744412397
proliferation and growth of intestinal epithelium in ascaris suum (nematoda) during postnatal ontogeny. i. mitotic activity in the intestinal epithelium. 19744428217
the secretory activity of ascaris suum intestine. 19744436769
isolation of somatic antigen from second stage larvae of ascaris suum. 19744448434
[ultrastructure of the nervous system of ascaris suum]. 19744449650
[content of vitamin a fractions in the tissues of ascaris suum]. 19744456277
proliferation and growth of intestinal epithelium in ascaris suum (nematoda) during postnatal ontogeny. ii. increase in the number and size of cells as a growth factor. 19744471355
[effect of fusarium genus fungi on the development of eggs of ascaris suum goeze]. 19744476130
[pathomorphological changes in the organs of guinea pigs repeatedly infected with eggs of ascaris suum goeze, 1782]. 19744477889
experimental ascaris suum infection in piglets. inverse relationship between the numbers of inoculated eggs and the numbers of worms established in the intestine. 19734521966
[effect of performic, peracetic and perpropionic acids on the eggs of ascaris suum in vitro]. 19724522060
observations on the relationship between ascaris suum burdens in pigs and faecal egg counts. 19744533224
eosinophil response to migrating ascaris suum larvae in normal and congenitally thymus-less mice. 19744533228
isolation of a heme protein antigen from ascaris suum. 19724624939
biological techniques for studying the allergenic components of nematodes. ii. the characterisation of the allergen released by ascaris suum maintaind in saline. 19734633027
purification and properties of fructose diphosphate aldolase from ascaris suum muscle. 19724635793
[determination of the immunoglobulin classes in rabbit serum experimentally infected with ascaris suum]. 19724658902
age polyploidization of uterine epithelial cells of ascaris suum (nematoda). 19724668013
experimental induction of esophagogastric ulcers with inoculations of ascaris suum eggs in swine. 19724672292
[studies on vitamin b 12 -dependent reactions in ascaris suum]. 19724673689
biological techniques for studying the allergenic components of nematodes. i. detection of allergenic components in ascaris suum extracts. 19724681750
subcellular distribution of digestive enzymes in ascaris suum intestine. 19734687435
ascaris suum: a scanning electron microscope study. 19734687487
transport of 3-0-methylglucose by various regions of the intestine of ascaris suum. 19734687503
neutral alkoxylipids of ascaris suum. 19734687504
ascaris suum: aldolase i purification and immunologic study. 19734691930
effect of mycoplasma hyopneumoniae infections, pyrantel treatment and protein nutrition on performance of pigs exposed to soil containing ascaris suum ova. 19734703718
resistance of pigs recovered from transmissible gastroenteritis against infection with ascaris suum. 19734707563
conversion of ascaroside esters to free ascarosides in fertilized eggs of ascaris suum (nematoda). 19734711661
ascaris lumbricoides linné, 1758 and ascaris suum goeze, 1782: morphological differences between specimens obtained from man and pig. 19734718127
anticholinesterase activity of piperazine derivatives. study of the structure-activity relationship in piperazine derivatives measured by the potentiation of acetylcholine response in ascaris suum. 19734728098
on the development of interstitial hepatitis ("milk spots") in pigs following infection with ascaris suum. 19734728775
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