| differential protein expression marks the transition from infection with opisthorchis viverrini to cholangiocarcinoma. | parts of southeast asia have the highest incidence of intrahepatic cholangiocarcinoma (cca) in the world due to infection by the liver fluke opisthorchis viverrini (ov). ov-associated cca is the culmination of chronic ov-infection, with the persistent production of the growth factors and cytokines associated with persistent inflammation, which can endure for years in ov-infected individuals prior to transitioning to cca. isobaric labelling and tandem mass spectrometry of liver tissue from a hams ... | 2017 | 28232516 |
| changing patterns of prevalence in opisthorchis viverrini sensu lato infection in children and adolescents in northeast thailand. | infection with the liver fluke opisthorchis viverrini sensu lato (s.l.), a group 1 carcinogen, is the most important risk factor for developing cholangiocarcinoma (cca) in southeast asia. cholangiocarcinoma is a fatal disease with the world's highest incidence being found in northeast thailand. liver fluke infection occurs through eating raw or partially cooked cyprinid fish containing metacercariae and, therefore, the control of o. viverrini s.l. infection should lead to a reduction in cca inci ... | 2016 | 27794488 |
| reinfection by opisthorchis viverrini after treatment with praziquantel. | the prevalence of infection by the liver fluke, opisthorchis viverrini (o. viverrini), has remained high in northeast thailand where it is a major risk factor for the eventual development of cholangiocarcinoma (cca). the infection is acquired by the consumption of dishes containing unsafely prepared freshwater fish, a dietary tradition which has proved resistant to change. since many people are aware that dosing with praziquantel (pzq) is a successful treatment for an episode of the infection, t ... | 2016 | 26925692 |
| efficacy and safety of praziquantel against light infections of opisthorchis viverrini: a randomised parallel single blind dose-ranging trial. | the liver fluke opisthorchis viverrini, highly prevalent in southeast asia, is an important public health burden, including a risk factor for developing an aggressive bile duct cancer, cholangiocarcinoma, in chronically infected patients. praziquantel, administered at single 40 mg/kg in preventive chemotherapy programs and 3 x 25 mg/kg for individual treatment is the drug of choice, yet information on the nature of the dose-response relationship is lacking. | 2016 | 27927864 |
| role of socio-cultural and economic factors in cyprinid fish distribution networks and consumption in lawa lake region, northeast thailand: novel perspectives on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is a fish-borne parasite endemic in parts of lao pdr, cambodia, southern vietnam and northeast thailand (isaan) where an estimated 10 million people are infected. human ov infection, associated with hepatobiliary complications, including cholangiocarcinoma (cca), occurs when infected fish are consumed raw or undercooked, a longstanding cultural tradition in the region. this mixed- methods descriptive study was carried out in isaan villages around lawa lake, khon kaen ... | 2017 | 28216369 |
| co-infections with liver fluke and helicobacter species: a paradigm change in pathogenesis of opisthorchiasis and cholangiocarcinoma? | infection with the fish-borne liver fluke opisthorchis viverrini is classified by the international agency for research on cancer as a group 1 carcinogen: definitely carcinogenic in humans. cofactors likely contribute to bile duct cancer (cholangiocarcinoma) caused by this infection. here we review recent findings that address the role of liver fluke associated h. pylori in hepatobiliary disease and malignancy. we hypothesize that co-infection by o. viverrini and the bacillus helicobacter pylori ... | 2016 | 27919744 |
| modeling impacts of climate change on the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. | global climate change is now regarded as imposing a significant threat of enhancing transmission of parasitic diseases. maximum entropy species distribution modeling (maxent) was used to explore how projected climate change could affect the potential distribution of the carcinogenic liver fluke, opisthorchis viverrini, in thailand. a range of climate variables was used: the hadley global environment model 2-earth system (hadgem2-es) climate change model and also the ipcc scenarios a2a for 2050 a ... | 2017 | 27774575 |
| elevated prevalence of helicobacter species and virulence factors in opisthorchiasis and associated hepatobiliary disease. | recent reports suggest that opisthorchis viverrini serves as a reservoir of helicobacter and implicate helicobacter in pathogenesis of opisthorchiasis-associated cholangiocarcinoma (cca). here, 553 age-sex matched cases and controls, 293 and 260 positive and negative for liver fluke o. viverrini eggs, of residents in northeastern thailand were investigated for associations among infection with liver fluke, helicobacter and hepatobiliary fibrosis. the prevalence of h. pylori infection was higher ... | 2017 | 28198451 |
| association between opisthorchis viverrini and leptospira spp. infection in endemic northeast thailand. | opisthorchiasis caused by opisthorchis viverrini is an important foodborne trematodiasis in thailand, laos and cambodia. interestingly, the opisthorchiasis endemic region overlaps with an area of leptospirosis emergence. here we report an association between opisthorchiasis and leptospirosis in thailand. of 280 sera collected from villagers living around the lawa wetland complex in khon kaen province, 199 (71%) were seropositive for leptospirosis by immunochromatography. individuals with o. vive ... | 2016 | 27746382 |
| efficacy and safety of tribendimidine against opisthorchis viverrini: two randomised, parallel-group, single-blind, dose-ranging, phase 2 trials. | treatment of the liver fluke infection opisthorchis viverrini relies exclusively on praziquantel. tribendimidine could be an alternative treatment option. we aimed to assess the efficacy and safety of ascending single, oral doses of tribendimidine in patients with o viverrini infection. | 2016 | 27472949 |
| differential protein expression in the hemolymph of bithynia siamensis goniomphalos infected with opisthorchis viverrini. | bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke opisthorchis viverrini. this parasite is a major public health problem in different countries throughout the greater mekong sub-region (thailand, southern vietnam, lao pdr and cambodia). chronic o. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma ... | 2016 | 27893749 |
| morphological and molecular identification of the liver fluke opisthorchis viverrini in the first intermediate host bithynia snails and its prevalence in kampong cham province, cambodia. | | 2017 | 28189767 |
| application of environmental dna analysis for the detection of opisthorchis viverrini dna in water samples. | opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in southeast asian countries. the trematode, opisthorchis viverrini, is the causative agent of the disease. accurate and rapid monitoring of o. viverrini is crucial for disease prevention and containment. therefore, in this study we sought to develop a novel species-specific real-time pcr assay for detecting o. viverrini using environmental dna (edna). the diagnostic sensitivity ... | 2017 | 28108370 |
| a combination of liver fluke infection and traditional northeastern thai foods associated with cholangiocarcinoma development. | opisthorchis viverrini infection is one of the risk factors for cholangiocarcinoma (cca) in northeast thailand, a region with one of the highest reported incidence rates of cca. the traditional practice of eating raw fish, repeated exposure to liver flukes, and consumption of nitrosamine-contaminated food are major risk factors for cca. so far, there have been no reports about which northeastern traditional dishes may be involved in cca development. the present study, thus, investigated the effe ... | 2016 | 27271702 |
| overweight relation to liver fluke infection among rural participants from 4 districts of nakhon ratchasima province, thailand. | a cross-sectional survey was conducted among 730 participants from 4 rural districts of nakhon ratchasima province, thailand, with a reported high incidence of liver fluke infection. this study was aimed to examine and evaluate the nutritional status in relation to opisthorchis viverrini infection. participants were purposive selected from chum phuang, mueang yang, bua yai, and kaeng sanam nang districts. stool samples were prepared by kato katz technique and then assessed by microscopy. anthrop ... | 2016 | 27268631 |
| assessing the role of landscape connectivity on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is one of the most important human parasitic diseases in southeast asia. although the concept of connectivity is widely used to comprehend disease dispersal, knowledge of the influences of landscape connectivity on ov transmission is still rudimentary. this study aimed to investigate the role of landscape connectivity in ov transmission between the human and the first intermediate snail hosts. fieldwork was conducted in three villages respectively in kamalasai distric ... | 2016 | 27265875 |
| wound healing and cancer progression in opisthorchis viverrini associated cholangiocarcinoma. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). it was shown previously that o. viverrini-secreted proteins accelerate wound resolution in human cholangiocytes. recombinant ov-grn-1 (o. viverrini-derived gene encoding granulin-like growth factor) induced angiogenesis and accelerated mouse wound healing. given the striking similarities of wound healing and cancer progression, here we discuss the major implications of this fin ... | 2016 | 27130317 |
| nurses and television as sources of information effecting behavioral improvement regarding liver flukes in nakhon ratchasima province, thailand. | liver fluke infection with opisthorchis viverrini and its associated cholangiocarcinoma constitute a serious problem in thailand. healthy behavior can decrease infection, therefore, the investigation of knowledge, attitude, and practice is need required in high risk areas. | 2016 | 27039731 |
| pipeline for the identification and classification of ion channels in parasitic flatworms. | ion channels are well characterised in model organisms, principally because of the availability of functional genomic tools and datasets for these species. this contrasts the situation, for example, for parasites of humans and animals, whose genomic and biological uniqueness means that many genes and their products cannot be annotated. as ion channels are recognised as important drug targets in mammals, the accurate identification and classification of parasite channels could provide major prosp ... | 2016 | 26983991 |
| cytokine profiles in opisthorchis viverrini stimulated peripheral blood mononuclear cells from cholangiocarcinoma patients. | the carcinogenic liver fluke opisthorchis viverrini causes chronic inflammation in the bile duct and resulting in unremitting tissue damage that lead to hepatobiliary diseases, including cholangiocarcinoma (cca). despite inflammatory cytokine expression having been studied in the animal model, so far no studies have been carried out on cytokines in human cca cases. here we report the profile of cytokine production in peripheral blood mononuclear cells (pbmcs) collected from o. viverrini-associat ... | 2017 | 27769807 |
| proteomic characterization of the internalization of opisthorchis viverrini excretory/secretory products in human cells. | the association between liver fluke infection caused by opisthorchis viverrini and cholangiocarcinoma (cca - hepatic cancer of the bile duct epithelium) has been well established. multiple mechanisms play a role in the development of cca, but the excretory/secretory products released by o. viverrini (oves) represent the major interface between the parasite and its host, and their uptake by biliary epithelial cells has been suggested to be responsible for proliferation of cholangiocytes, the cell ... | 2016 | 26873540 |
| cpg oligodeoxynucleotides with crude parasite antigens reduce worm recovery in opisthorchis viverrini infected hamsters. | opisthorchis viverrini, a human liver fluke, is still an endemic parasitic infection in thailand and nearly all countries in southeast asia. o. viverrini induces a chronic stage of infection in hamsters. during the first 2 weeks of infection, th1 inducing cytokine, il-12, increased but was down regulated in chronic infection. in this study it was found that unmethylated-cpg odn (oligodeoxynucleotides) 1826 increased hamster mononuclear cell proliferation and stimulated ifn-γ production in vitro. ... | 2016 | 27737764 |
| malacological investigation of the fully operational nam theun 2 hydroelectric dam project in khammouane province, central lao pdr. | we conducted a malacological investigation in four districts of the nam theun 2 (nt2) hydroelectric dam project area, khammouane province, central lao pdr (nakai, gnommalath, mahaxai and xe bang fai), after the first and second years of full operation in march 2010 and november 2011 to determine health risks for humans. a total 10,863 snail specimens (10 families/23 species) from 57 sampling stations and 12,902 snail specimens (eight families/21 species) from 66 sampling stations were collected ... | 2015 | 26863858 |
| excretory/secretory products of the carcinogenic liver fluke are endocytosed by human cholangiocytes and drive cell proliferation and il6 production. | liver fluke infection caused by opisthorchis viverrini remains a major public health problem in many parts of asia including thailand, lao pdr, vietnam and cambodia, where there is a strikingly high incidence of cholangiocarcinoma (cca - hepatic cancer of the bile duct epithelium). among other factors, uptake of o. viverrini excretory/secretory products (oves) by biliary epithelial cells has been postulated to be responsible for chronic inflammation and proliferation of cholangiocytes, but the m ... | 2015 | 26187786 |
| people participation towards opisthorchis viverrini prevention and control in chaiyaphum province, northeastern thailand. | this cross-sectional descriptive and qualitative study was aimed to study the people participation and their approaches toward the human carcinogenic liver fluke, opisthorchis viverrini, prevention and control in ban chaun sub-district administrative organization (bcsao) and bamnet narong sub-district municipality (bnsm), bamnet narong district, chaiyaphum province, thailand between june 2013 and february 2014. participants were purposive selected, included chiefs of sub-district administrative ... | 2016 | 26838206 |
| untangling the complexity of liver fluke infection and cholangiocarcinoma in ne thailand through transdisciplinary learning. | this study demonstrates how a transdisciplinary learning approach provided new insights for explaining persistent opisthorchis viverrini infection in northern thailand, as well as elucidating problems of focusing solely on the parasite as a means of addressing high prevalence of cholangiocarcinoma. researchers from diverse backgrounds collaborated to design an investigative homestay program for 72 singaporean and thai university students in five northeast thai villages. the students explored how ... | 2016 | 26822781 |
| xanthohumol inhibits stat3 activation pathway leading to growth suppression and apoptosis induction in human cholangiocarcinoma cells. | stat3 plays a significant role in the development of cholangiocarcinoma (cca) associated with the liver fluke (opisthorchis viverrini; ov). xanthohumol (xn), a prenylated flavonoid extracted from hops, has known anticancer activity and could potentially target stat3. the present study determined the effect of xn on stat3, as well as ascertained its usefulness against cca. the cca cell proliferation at 20 µm and 50 µm of xn was shown to inhibited, while 20 µm partially inhibited il-6-induced stat ... | 2016 | 26794001 |
| genomics of worms, with an emphasis on opisthorchis viverrini - opportunities for fundamental discovery and biomedical outcomes. | neglected tropical diseases cause substantial morbidity and mortality in animals and people globally. opisthorchiasis is one such disease, caused by the carcinogenic, asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is known to be associated with malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia, including thailand, lao people's democratic republic (pdr) and cambodia. no vaccine is available, and only one drug (praziquantel) is routinely emplo ... | 2016 | 26792076 |
| increase in l-type amino acid transporter 1 expression during cholangiocarcinogenesis caused by liver fluke infection and its prognostic significance. | l-type amino acid transporter 1 (lat1) is highly expressed in various human cancers, including cholangiocarcinoma (cca), the most common cancer in northeast thailand. chronic inflammation and oxidative stress induced by liver fluke, opisthorchis viverrini, infection has been recognized as the major cause of cca in this area. we show here that an increased expression of lat1 and its co-functional protein cd98 are found during carcinogenesis induced by ov in hamster cca tissues. we also demonstrat ... | 2015 | 26657242 |
| modifying health behavior for liver fluke and cholangiocarcinoma prevention with the health belief model and social support theory. | the liver fluke opisthorchis viverrini is a serious health problem in thailand. infection is associated with cholangiocarcinoma (cca), endemic among human populations in northeast and north thailand where raw fish containing fluke metacercariae are frequently consumed. recently, thailand public health authorities have been organized to reduce morbidity and mortality particularly in the northeast through o. viverrini and cca screening projects. health modfication is one of activities included in ... | 2016 | 27644606 |
| the carcinogenic liver fluke opisthorchis viverrini among rural community people in northeast thailand: a cross- sectional descriptive study using multistage sampling technique. | opisthorchis viverrini infection is a serious public health problem in southeast asia especially in the northeast and north of thailand. therefore, a cross-sectional survey using multistage sampling was conducted from the rural communities of surin province, thailand, during september 2013 to july 2014. o. viverrini infection was determined using kato's thick smear technique. socio-demographic, information resources, and history data were collected using predesigned semi-structured questionnaire ... | 2015 | 26625801 |
| population pharmacokinetic modeling of tribendimidine metabolites in opisthorchis viverrini-infected adults. | there is a pressing need for alternative treatments against the liver fluke opisthorchis viverrini oral tribendimidine is a promising candidate, but its population pharmacokinetic properties are unknown. two phase iia trials were conducted in laos in o. viverrini-infected adults receiving single oral doses of 25 to 600 mg tribendimidine administered as different formulations in each study (study 1 used 200-mg tablets, and study 2 used 50-mg tablets). venous whole blood, plasma, and capillary dri ... | 2016 | 27431233 |
| effects of fermentation time and low temperature during the production process of thai pickled fish (pla-som) on the viability and infectivity of opisthorchis viverrini metacercariae. | contamination of a popular fermented fish dish, pla-som, by opisthorchis viverrini metacercariae (ovmc) is a possible cause of carcinogenic liver fluke infection in thailand. affected individuals are at risk of bile duct cancer, which is a major health problem for people in the greater mekong subregion. in order to investigate concerns about food safety, we studied the effects of fermentation time and low temperature on the viability and infectivity of ovmc during the pla-som production process. ... | 2016 | 26587967 |
| tissue microbiome profiling identifies an enrichment of specific enteric bacteria in opisthorchis viverrini associated cholangiocarcinoma. | cholangiocarcinoma (cca) is the primary cancer of the bile duct system. the role of bile duct tissue microbiomes in cca tumorigenesis is unestablished. to address this, sixty primary cca tumors and matched normals, from both liver fluke (opisthorchis viverrini) associated (ova, n=28) and non-o. viverrini associated (non-ova, n=32) cancers, were profiled using high-throughput 16s rrna sequencing. a distinct, tissue-specific microbiome dominated by the bacterial families dietziaceae, pseudomonadac ... | 2016 | 27428430 |
| development and validation of an enantioselective lc-ms/ms method for the analysis of the anthelmintic drug praziquantel and its main metabolite in human plasma, blood and dried blood spots. | praziquantel (pzq) is the treatment of choice against various trematode and cestode infections. to study the pharmacokinetics of pzq in patients infected with the liver fluke opisthorchis viverrini, we developed and validated an enantioselective liquid chromatography coupled to tandem mass spectrometry method for the analysis of r - and s -pzq and its r -trans-4-oh-pzq metabolite in human plasma, blood and dried blood spots (dbs). the analytes were detected in the positive mode using selected re ... | 2016 | 26517852 |
| association between praziquantel treatment and cholangiocarcinoma: a hospital-based matched case-control study. | infection by the liver fluke, opisthorchis viverrini, remains an important public health problem in thailand and has resulted in the highest prevalence of infection and incidence of subsequent cholangiocarcinoma (cca) in the world. praziquantel (pzq) is the antihelminthic drug of choice for treatment. previous studies in hamsters showed that repeated infection and pzq treatment could increase the risk of cca. however, the few available epidemiology studies in humans have shown unclear evidence o ... | 2015 | 26496745 |
| carcinogenic parasite secretes growth factor that accelerates wound healing and potentially promotes neoplasia. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). injury from feeding activities of this parasite within the human biliary tree causes extensive lesions, wounds that undergo protracted cycles of healing, and re-injury over years of chronic infection. we show that o. viverrini secreted proteins accelerated wound resolution in human cholangiocytes, an outcome that was compromised following silencing of expression of the fluke-de ... | 2015 | 26485648 |
| cd44 variant-dependent redox status regulation in liver fluke-associated cholangiocarcinoma: a target for cholangiocarcinoma treatment. | expression of cd44, especially the variant isoforms (cd44v) of this major cancer stem cell marker, contributes to reactive oxygen species (ros) defense through stabilizing xct (a cystine-glutamate transporter) and promoting glutathione synthesis. this enhances cancer development and increases chemotherapy resistance. we investigate the role of cd44v in the regulation of the ros defense system in cholangiocarcinoma (cca). immunohistochemical staining of cd44v and p38(mapk) (a major ros target) ex ... | 2016 | 27176078 |
| pharmacokinetic study of praziquantel enantiomers and its main metabolite r-trans-4-oh-pzq in plasma, blood and dried blood spots in opisthorchis viverrini-infected patients. | praziquantel (pzq) is the treatment of choice for infections with the liver fluke opisthorchis viverrini, a major health problem in southeast asia. however, pharmacokinetic (pk) studies investigating the disposition of pzq enantiomers (r- and s-pzq) and its main metabolite, r-trans-4-oh-pzq, in diseased patients are lacking. the implementation of a dried blood spot (dbs) sampling technique would ease the performance of pk studies in remote areas without clinical facilities. the aim of the presen ... | 2016 | 27152952 |
| decreased risk of cholangiocarcinogenesis following repeated cycles of opisthorchis viverrini infection-praziquantel treatment: magnetic resonance imaging (mri) and histopathological study in a hamster model. | it has been suggested that repeated infection of opisthorchis viverrini followed by repeated treatment with praziquantel (pzq) increases risk of development of cholangiocarcinoma (cca). evidence for the prediction has accumulated based on findings of indirect approaches involving molecular changes and epidemiological trends. by contrast, here we directly monitored the impact of repeated liver fluke infection and treatment with pzq on cholangiocarcinogenesis in a rodent model of human opisthorchi ... | 2016 | 27143606 |
| urinary microrna-192 and microrna-21 as potential indicators for liver fluke-associated cholangiocarcinoma risk group. | opisthorchis viverrini infection induces chronic inflammation in the bile ducts, leading to periductal fibrosis (pdf), which possibly associates to cholangiocarcinoma (cca). patients with cca have a poor prognosis, which is linked to asymptomatic disease and late diagnosis. hence, detecting early stage cca is essential. secretory mirnas have been promoted as biomarkers for pathological changes associated with parasitic infections, fibrosis and/or cancer. we aimed to determine levels of mir-192 a ... | 2015 | 26456596 |
| proteomic profile of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini. | the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. the undisputed link between cca and o. viverrini infection has precipitated efforts to understand the molecular basis of host-parasite interactions with a view to ultimately developing new control strategies to combat this carcinogenic infection. to date most effort has focused on the interactions betwee ... | 2015 | 25284051 |
| upregulation of 14-3-3 eta in chronic liver fluke infection is a potential diagnostic marker of cholangiocarcinoma. | to discover protein markers in chronic/advanced opisthorchiasis for the early detection of opisthorchis viverrini (ov)-associated cholangiocarcinoma (cca). | 2016 | 26435198 |
| pathological characteristics of intraductal polypoid neoplasms of bile ducts in thailand. | intraductal papillary or tubular neoplasms of the bile duct have recently been proposed as one of the pre-invasive lesions of cholangiocarcinoma. herein, a total of 50 cases of intraluminal polypoid neoplasms of the bile ducts experienced in khon kaen university hospital in thailand were pathologically examined. these cases presumably had a history of infection of opisthorchis viverrini. these neoplasms were histologically composed of high-grade intraepithelial neoplasm showing a tubular and/pap ... | 2015 | 26339398 |
| data set from the proteomic analysis of bithynia siamensis goniomphalos snails upon infection with the carcinogenic liver fluke opisthorchis viverrini. | the snail bithynia siamensis goniomphalos acts as the first intermediate host for the human liver fluke opisthorchis viverrini, the major cause of cholangiocarcinoma (cca) in northeast thailand. this data article contains the results obtained from the analysis of the proteins differentially expressed in the snail b. siamensis goniomphalos upon infection with o. viverrini. it contains the data generated from iquantitator software including a pdf of each sample with a protein׳s relative expression ... | 2015 | 26217698 |
| risk areas of liver flukes in surin province of thailand using geographic information system. | opisthorchiasis, caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao pdr and thailand. | 2015 | 26201130 |
| localization of tubulin from the carcinogenic human liver fluke, opisthorchis viverrini. | opisthorchiasis caused by opisthorchis viverrini, is of considerable public health importance in southeast asia, particularly in lao people's democratic republic and thailand. the infection is associated with a number ofhepatobiliary diseases including cholangitis, obstructive jaundice, hepatomegaly, cholecystitis, cholelithiasis and cholangiocarcinoma. | 2015 | 26201128 |
| [a 55-year old thai male patient with acute right upper quadrant abdominal pain]. | we report on a 55-year-old thai male who presented during a visit to germany with acute abdominal pain in the right upper quadrant and signs of cholestasis. the diagnosis of liver fluke infection (opisthorchiasis) was confirmed by the detection of the characteristic eggs of opisthorchis viverrini in stool microscopy. the patient was treated with praziquantel. opisthorchiasis is a common parasitic disease and major public health problem in southeast asia and in eastern europe. | 2015 | 26116106 |
| apoptosis of cholangiocytes modulated by thioredoxin of carcinogenic liver fluke. | chronic infection with the food-borne liver fluke, opisthorchis viverrini, frequently induces cancer of the bile ducts, cholangiocarcinoma. opisthorchiasis is endemic in thailand, lao pdr, cambodia and vietnam, where eating undercooked freshwater fish carrying the juvenile stage of this pathogen leads to human infection. because inhibition of apoptosis facilitates carcinogenesis, this study investigated modulation by thioredoxin from o. viverrini of apoptosis of bile duct epithelial cells, chola ... | 2015 | 26007234 |
| trematode diversity in the freshwater snail bithynia siamensis goniomphalos sensu lato from thailand and lao pdr. | in order to obtain a comprehensive understanding of trematode diversity in bithynia siamensis goniomphalos sensu lato, the first intermediate host of the liver fluke opisthorchis viverrini s.l., the prevalence of larval trematode species was investigated in different localities in thailand and lao people's democratic republic (lao pdr). in thailand, snail samples were collected from 29 localities in the nine provinces: buri ram, surin, chaiya phum, maha sarakham, khon kaen, kalasin, mukdahan, sa ... | 2016 | 25997613 |
| carcinogenic liver fluke secretes extracellular vesicles that promote cholangiocytes to adopt a tumorigenic phenotype. | throughout asia, there is an unprecedented link between cholangiocarcinoma and infection with the liver fluke opisthorchis viverrini. multiple processes, including chronic inflammation and secretion of parasite proteins into the biliary epithelium, drive infection toward cancer. until now, the mechanism and effects of parasite protein entry into cholangiocytes was unknown. | 2015 | 25985904 |
| re-examination of opisthorchis viverrini infection in northeast thailand. | liver fluke infection caused by the parasite opisthorchis viverrini (o. viverrini), a human carcinogen, is endemic in north-eastern thailand and remains a major health problem. | 2015 | 25921154 |
| diabetes mellitus: possible risk and promoting factors of cholangiocarcinoma: association of diabetes mellitus and cholangiocarcinoma. | the highest incidence of cholangiocarcinoma (cca), a malignancy of bile duct epithelia, is in the northeast of thailand. the liver fluke, opisthorchis viverrini, is the known risk factor for cca development in this region. approximately 1% of o. viverrini infected individuals develop cca. there could be other factors that influence the cholangiocarcinogenesis particularly in the o. viverrini infected individuals. the global epidemiological studies of risk factors for cca in non-o. viverrini rela ... | 2015 | 25910864 |
| anthelmintic, anti-inflammatory and antioxidant effects of garcinia mangostana extract in hamster opisthorchiasis. | administration of praziquantel for treatment of liver fluke infection may affect the host, with mild and severe effects after treatment caused by host immune response. therefore, we focused on the antioxidant property, inflammatory and anthelmintic effects of the traditional folk medicine, g. mangostana pericarp extract, in hamster opisthorchiasis. syrian hamsters were divided into four groups: normal (control) (n); administered g. mangostana alone (gm); infected with opisthorchis viverrini alon ... | 2015 | 25836376 |
| gis-based spatial statistical analysis of risk areas for liver flukes in surin province of thailand. | it is urgently necessary to be aware of the distribution and risk areas of liver fluke, opisthorchis viverrini, for proper allocation of prevention and control measures. this study aimed to investigate the human behavior, and environmental factors influencing the distribution in surin province of thailand, and to build a model using stepwise multiple regression analysis with a geographic information system (gis) on environment and climate data. the relationship between the human behavior, attitu ... | 2015 | 25824758 |
| plasma igg autoantibody against actin-related protein 3 in liver fluke opisthorchis viverrini infection. | opisthorchiasis secondary to opisthorchis viverrini infection leads to cholangiocellular carcinoma through chronic inflammation of the bile ducts and possibly inducing autoimmunity. it was hypothesized that plasma autoantibodies directed against self-proteins are biomarkers for opisthorchiasis. plasma from patients with opisthorchiasis was tested using proteins derived from immortalized cholangiocyte cell lines, and spots reacting with plasma were excised and subjected to lc-ms/ms. seven protein ... | 2015 | 25809205 |
| the carcinogenic liver fluke opisthorchis viverrini is a reservoir for species of helicobacter. | there has been a strong, positive correlation between opisthorchiasis-associated cholangiocarcinoma and infection with helicobacter. here a rodent model of human infection with opisthorchis viverrini was utilized to further investigate relationships of apparent co-infections with o. viverrini and h. pylori. a total of 150 hamsters were assigned to five groups: i) control hamsters not infected with o. viverrini; ii) o. viverrini-infected hamsters; iii) non-o. viverrini infected hamsters treated w ... | 2015 | 25773821 |
| genetics of opisthorchis viverrini-related cholangiocarcinoma. | we review the genetic, epigenetic and transcriptional landscape of liver fluke (opisthorchis viverrini, ov)-related cholangiocarcinoma (cca). its distinct alterations, as compared with non-ov-related cca may help shed light on its underlying molecular mechanisms. | 2015 | 25693006 |
| bile canalicular changes and defective bile secretion in opisthorchis viverrini-infected hamsters. | infection with the liver fluke opisthorchis viverrini (digenea) (poirier, 1886) causes bile duct injury and periductal fibrosis by chronic overproduction of inflammatory-mediators and eventually results in cholangiocarcinoma development. while extensive research works have been done on o. viverrini infection-associated changes of bile ducts and periductal fibrosis, little attention was paid on morphological and biochemical changes of the bile canaliculi (bc), the origin of bile flow. we aimed to ... | 2014 | 25651692 |
| opisthorchis viverrini infection activates the pi3k/ akt/pten and wnt/β-catenin signaling pathways in a cholangiocarcinogenesis model. | opisthorchis viverrini (ov) infection is the major etiological factor for cholangiocarcinoma (cca), especially in northeast thailand. we have previously reported significant involvement of pi3k/akt/pten and wnt/β- catenin in human cca tissues. the present study, therefore, examined the expression and activation of pi3k/ akt/pten and wnt/β-catenin signaling components during ov-induced cholangiocarcinogenesis in a hamster animal model. hamsters were divided into two groups; non-treated and ov plu ... | 2014 | 25556493 |
| bi-functionality of opisthorchis viverrini aquaporins. | aquaporins (aqp) are essential mediators of water regulation in all living organisms and members of the major intrinsic protein (mip) superfamily of integral membrane proteins. they are potential vehicles or targets for chemotherapy, e.g. in trypanosoma brucei melarsoprol and pentamidine uptake is facilitated by tbaqp-2. transcriptome data suggests that there are at least three active aquaporins in the human liver fluke, opisthorchis viverrini, ovaqp-1, 2 and 3, and crude rna silencing of ovaqp- ... | 2015 | 25461277 |
| advances in the diagnosis of human opisthorchiasis: development of opisthorchis viverrini antigen detection in urine. | many strategies to control opisthorchiasis have been employed in thailand, but not in the other neighbouring countries. specific control methods include mass drug administration (mda) and health education to reduce raw fish consumption. these control efforts have greatly shifted the epidemiology of opisthorchis viverrini (ov) infection over the last decade from presenting as densely concentrated "heavy" infections in single villages to widespread "light" ov infections distributed over wide geogr ... | 2015 | 26485024 |
| the mirnaome of opisthorchis viverrini induced intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is an aggressive cancer, arising in the biliary ducts that extend into the liver. the highest incidence of icc occurs in southeast asia, particularly in the mekong river basin countries of thailand, laos, cambodia, and vietnam, where it is strongly associated with chronic infection by the food-borne liver fluke opisthorchis viverrini (ov), one of only three eukaryote pathogens considered group one carcinogens. intrahepatic cholangiocarcinoma is usually diagn ... | 2014 | 26484108 |
| suppression of ov-grn-1 encoding granulin of opisthorchis viverrini inhibits proliferation of biliary epithelial cells. | multistep processes likely underlie cholangiocarcinogenesis induced by chronic infection with the fish-borne liver fluke, opisthorchis viverrini. one process appears to be cellular proliferation of the host bile duct epithelia driven by excretory-secretory (es) products of this pathogen. specifically, the secreted growth factor ov-grn-1, a liver fluke granulin, is a prominent component of es and a known driver of hyper-proliferation of cultured human and mouse cells in vitro. we show potent hype ... | 2015 | 25450776 |
| genetic and environmental determinants of risk for cholangiocarcinoma in thailand. | cholangiocarcinoma (cca) is a difficult cancer to diagnose in the early stage and to treat by curative resection. the incidence of cca in the northeast of thailand is the highest in the world. to make progress in detecting a high risk group and in the prevention and detection of cca, we have been analyzing the risk factors for cca. although liver fluke infection is known to be a risk factor, there are patients who are not infected with the liver fluke and not all people infected with the liver f ... | 2014 | 25401000 |
| immunization and challenge shown by hamsters infected with opisthorchis viverrini following exposure to gamma-irradiated metacercariae of this carcinogenic liver fluke. | here we report findings to optimize and standardize conditions to attenuate metacercariae of opisthorchis viverrini by ionizing radiation to elicit protective immune responses to challenge infection. metacercariae were gamma-irradiated and the ability of irradiated metacercariae to prevent patent infection of challenge metacercariae in hamsters was determined, as well as their ability to induce a host antibody response. metacercariae irradiated in a dose-dependent manner, with 3, 5, 10, 12, 20, ... | 2016 | 25315797 |
| subtle to severe hepatobiliary morbidity in opisthorchis viverrini endemic settings in southern laos. | evidence of severe hepatobiliary morbidity associated with opisthorchis viverrini liver fluke infection including cholangiocarcinoma (cca) is scarce in laos although o. viverrini infection is highly prevalent. we assessed hepatobiliary morbidity using abdominal ultrasonography (us) in o. viverrini adult patients in saravan province, southern laos. a random sample of 431 o. viverrini patients from 10 villages underwent abdominal us. mild, moderate and markedly advanced periductal fibrosis was dia ... | 2015 | 25275346 |
| circulating mir-192 in liver fluke-associated cholangiocarcinoma patients: a prospective prognostic indicator. | this study aimed to investigate the mir-192 levels in patients' sera of liver fluke-associated cholangiocarcinoma (cca) for a prospective prognostic indicator. | 2014 | 25131257 |
| toward integrated opisthorchiasis control in northeast thailand: the lawa project. | human liver fluke, opisthorchis viverrini, a food-borne trematode is a significant public health problem in southeast asia, particularly in thailand. despite a long history of control programmes in thailand and a nationwide reduction, o. viverrini infection prevalence remains high in the northeastern provinces. therefore, a new strategy for controlling the liver fluke infection using the ecohealth/one health approach was introduced into the lawa lake area in khon kaen province where the liver fl ... | 2015 | 25102053 |
| stats profiling reveals predominantly-activated stat3 in cholangiocarcinoma genesis and progression. | we investigated the aberrant expression of the stat family in humans and liver fluke (opisthorchis viverrini, ov)-induced hamster cholangiocarcinoma (cca) tissues. | 2014 | 25044480 |
| an interleukin-6 receptor polymorphism is associated with opisthorchiasis-linked cholangiocarcinoma risk in thailand. | the cholangiocarcinoma (cca) is a relatively rare cancer worldwide but it is highly prevalent in thailand where the liver fluke, opisthorchis viverrini is endemic. there are reports that interleukin 6 (il-6) may play an important role in the pathogenesis of opisthorchiasis associated cca. functionally, il-6 can act on target cells through its receptor, il-6r, and il-6r polymorphisms may affect the functional activity of il-6 leading to susceptibility to cholangiocarcinogenesis. therefore, we ass ... | 2014 | 25041016 |
| distinct mirna signatures associate with subtypes of cholangiocarcinoma from infection with the tumourigenic liver fluke opisthorchis viverrini. | intrahepatic cholangiocarcinoma (icc) is a significant public health problem in east asia, where it is strongly associated with chronic infection by the food-borne parasite opisthorchis viverrini (ov). we report the first comprehensive mirna expression profiling by microarray of the most common histologic grades and subtypes of icc: well differentiated, moderately differentiated, and papillary icc. | 2014 | 25017828 |
| the opisthorchis viverrini genome provides insights into life in the bile duct. | opisthorchiasis is a neglected, tropical disease caused by the carcinogenic asian liver fluke, opisthorchis viverrini. this hepatobiliary disease is linked to malignant cancer (cholangiocarcinoma, cca) and affects millions of people in asia. no vaccine is available, and only one drug (praziquantel) is used against the parasite. little is known about o. viverrini biology and the diseases that it causes. here we characterize the draft genome (634.5 mb) and transcriptomes of o. viverrini, elucidate ... | 2014 | 25007141 |
| suppression of aquaporin, a mediator of water channel control in the carcinogenic liver fluke, opisthorchis viverrini. | opisthorchiasis and opisthorchis viverrini-associated bile duct cancer represent major public health threats in thailand and laos. the tegument of this food borne fluke plays pivotal roles in parasite metabolism, homeostasis and osmoregulation. excretory/secretory products also pass from the fluke to the biliary environment, products that likely underlie pathogenesis of liver fluke infection. aquaporins (aqps), belong to the major intrinsic protein superfamily of integral plasma membrane channel ... | 2014 | 24885060 |
| patterns of trematode infections of opisthorchis viverrini (opisthorchiidae) and haplorchis taichui (heterophyidae) in human populations from two villages in savannakhet province, lao pdr. | the liver fluke, opisthorchis viverrini, and the minute intestinal fluke, haplorchis taichui, are prevalent in many asian countries. this study analysed the patterns of infections of o. viverrini and h. taichui in lahanam and thakhamlien villages (savannakhet province, lao pdr), in two cross-sectional investigations. out of a total of 207 human participants, post-anthelmintic treatment positivity rates for expelled worms were 170 (82.1%) for h. taichui and 65 (31.4%) for o. viverrini. both these ... | 2015 | 24739959 |
| perceived illness drives participation in mass deworming campaigns in laos. | multiple helminth infections are very common in communities of southern laos. preventive chemotherapy in combination with health education is the mainstay of control. we assessed the knowledge, perceptions and practices of rural communities related to endemic helminthiasis and their control during a mass drug administration (mda) campaign. short interviews with household heads (n=192), direct observations and discussions with attendees of mdas, and in-depth interviews with local opinion leaders ... | 2015 | 24713201 |
| rna-seq reveals infection-induced gene expression changes in the snail intermediate host of the carcinogenic liver fluke, opisthorchis viverrini. | bithynia siamensis goniomphalos is the snail intermediate host of the liver fluke, opisthorchis viverrini, the leading cause of cholangiocarcinoma (cca) in the greater mekong sub-region of thailand. despite the severe public health impact of opisthorchis-induced cca, knowledge of the molecular interactions occurring between the parasite and its snail intermediate host is scant. the examination of differences in gene expression profiling between uninfected and o. viverrini-infected b. siamensis g ... | 2014 | 24676090 |
| an ecological study of bithynia snails, the first intermediate host of opisthorchis viverrini in northeast thailand. | infection with the food-borne trematodiasis, liver fluke opisthorchis viverrini, is a major public health concern in southeast asia. while epidemiology and parasitic incidence in humans are well studied, ecological information on the o. viverrini intermediate hosts remains limited. this study aimed to investigate the factors affecting the distribution and abundance of the first intermediate host, bithynia siamensis goniomphalos snails. water quality and snails were sampled in 31 sites in muang d ... | 2015 | 24561073 |
| genomic and transcriptional alterations of cholangiocarcinoma. | cholangiocarcinoma (cca) is one of the representative cancers refractory to any therapeutic approach. the incidence of cca is highest in the northeastern part of thailand, where chronic inflammation caused by liver fluke (opisthorchis viverrini: ov) infection is a major etiologic factor. the incidence of cca is also increasing in other countries, including japan. here, we overview the genetic and transcriptional alterations of cca with and without association with ov infection. cca with ov shows ... | 2014 | 24532422 |
| potential targeted therapy for liver fluke associated cholangiocarcinoma. | biliary tree cancer or cholangiocarcinoma (cca) is an unusual subtype of liver cancer with exceptionally poor prognosis. lack of specific symptoms and availability of early diagnostic markers account for late diagnosis of cca. surgical treatment is a gold standard choice but few patients are candidates and local recurrence after surgery is high. benefit of systemic chemotherapy is limited; hence, better treatment options are required. the differences in etiology, anatomical positions and patholo ... | 2014 | 24408866 |
| risk biomarkers for assessment and chemoprevention of liver fluke-associated cholangiocarcinoma. | human liver fluke, opisthorchis viverrini (ov), is the major risk factor of cholangiocarcinoma (cca) in northeastern thailand. our approach focuses on genetic progression and molecular changes in the carcinogenic pathway of liver fluke-associated cca aimed at assessing patients at risk of cca and using chemoprevention as the secondary cancer prevention to reduce the incidence of cca. this review summarizes altered gene expressions, biomolecules and their modification, i.e. dna adducts, oxidized ... | 2014 | 24408859 |
| association between praziquantel and cholangiocarcinoma in patients infected with opisthorchis viverrini: a systematic review and meta-analysis. | the liver fluke, opisthorchis viverrini, and the associated incidence of subsequent cholangiocarcinoma (cca) are still a public health problem in thailand, and praziquantel (pzq) remains the antihelminthic drug of choice for treatment. evidence in hamsters shows that repeated infection and pzq treatments could increase the risk of cca. however, the existing evidence in humans is inconclusive regarding increased risk of cca with frequency of pzq intake. | 2013 | 24377641 |
| endemicity of opisthorchis viverrini liver flukes, vietnam, 2011-2012. | | 2014 | 24377371 |
| exome sequencing identifies distinct mutational patterns in liver fluke-related and non-infection-related bile duct cancers. | the impact of different carcinogenic exposures on the specific patterns of somatic mutation in human tumors remains unclear. to address this issue, we profiled 209 cholangiocarcinomas (ccas) from asia and europe, including 108 cases caused by infection with the liver fluke opisthorchis viverrini and 101 cases caused by non-o. viverrini-related etiologies. whole-exome sequencing (n = 15) and prevalence screening (n = 194) identified recurrent somatic mutations in bap1 and arid1a, neither of which ... | 2013 | 24185513 |
| response to manuscript 'is opisthorchis viverrini an avian liver fluke?'. | | 2015 | 24160764 |
| is opisthorchis viverrini an avian liver fluke? | recently, in the journal of helminthology (may 2013), dao et al. reported that opisthorchis viverrini-like flukes were found in the bile duct of domestic ducks in vietnam. they stated that this is the first record of opisthorchis sp. in birds in vietnam. however, three opisthorchis species--o. cheelis, o. longissimus and o. parageminus--in birds in vietnam were described by le in 2000. amongst these, o. parageminus was first reported, by oshmarin in 1970, as a new opisthorchis species found in d ... | 2015 | 24160690 |
| carcinogenic liver fluke opisthorchis viverrini oxysterols detected by lc-ms/ms survey of soluble fraction parasite extract. | liquid chromatography in tandem mass spectrometry (lc-ms/ms) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. here, we used lc-ms/ms to investigate in soluble extracts of the adult developmental stage of opisthorchis viverrini from experimentally infected hamsters. using comparisons with known bile acids and the metabolites of estrogens, the lc-ms data indicated the existence of novel oxysterol derivatives ... | 2013 | 23973383 |
| infection with the carcinogenic liver fluke opisthorchis viverrini modifies intestinal and biliary microbiome. | opisthorchis viverrini is a fish-borne trematode endemic in east asia. following ingestion, the flukes locate to the biliary tre where chronic infection frequently leads to cholangiocarcinoma (cca). the mechanisms by which o. viverrini infection culminates in cca remain unknown. an unexplored aspect is its influence on the host microbiome. in the hamster, infection with this pathogen reliably leads to cca. genomic dnas of microbiota from colorectal contents and bile of hamsters and from whole o. ... | 2013 | 23925654 |
| infection with opisthorchis viverrini and use of praziquantel among a working-age population in northeast thailand. | infection with opisthorchis viverrini (ov) due to eating certain traditional freshwater fish dishes is the principal risk factor for cholangiocarcinoma in northeast thailand where the infection is endemic and the incidence of this form of primary liver cancer has been the highest in the world. this paper is the second report of a prospective research project to monitor the impacts of a national liver fluke control programme in a rural community of northeast thailand. a sample of 684 villagers ag ... | 2013 | 23803062 |
| melatonin inhibits cholangiocarcinoma and reduces liver injury in opisthorchis viverrini-infected and n-nitrosodimethylamine-treated hamsters. | the human liver fluke opisthorchis viverrini infection and n-nitrosodimethylamine (ndma) administration induce cholangiocarcinoma (cca) and liver injury in hamsters. melatonin protects against liver injury and reduces the alteration of mitochondrial structure, mitochondrial membrane potential, and mitochondrial pro- and anti-apoptotic pathways in various cancer types. to investigate the chemopreventive effect of melatonin on cca genesis and liver injury, hamsters were treated with a combination ... | 2013 | 23772655 |
| opisthorchis viverrini-like liver fluke in birds from vietnam: morphological variability and rdna/mtdna sequence confirmation. | flukes were found in the bile ducts of domestic ducks (anas platyrhynchos), necropsied in the binh dinh province of central vietnam. following staining, morphological characteristics of the bird flukes were compatible with opisthorchis viverrini, although some characteristics differed from those described in specimens collected from mammal hosts. computation of the phylogenetic trees on the partial sequences of the second internal ribosomal spacer (its2) of the ribosomal dna and cytochrome c oxi ... | 2014 | 23721954 |
| gene expression profiling of intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is ranked as one of the top five causes of cancer-related deaths. icc in thai patients is associated with infection with the liver fluke, opisthorchis viverrini, but the molecular basis for development remains unclear. the present study employed a microarray approach to compare gene expression profiles of iccs and normal liver tissues from the same patients residing in northeast thailand, a region with a high prevalence of liver fluke infection. in icc sampl ... | 2013 | 23534794 |
| alcohol and alkalosis enhance excystation of opisthorchis viverrini metacercariae. | the northeastern region of thailand has long been known as an endemic area of the human liver fluke infection which is caused by opisthorchis viverrini. humans are infected by ingestion of uncooked cyprinoid fish in traditional dishes such as "koi-pla," "pla-som," "pla-jom," and "pla-ra." to date, the prevalence of this parasite infection remains high because of cultural behavior and local beliefs. the popular misunderstanding among people in this area is that alcohol, lemon juice, and fish sauc ... | 2013 | 23460293 |
| overexpression of microrna-21 regulating pdcd4 during tumorigenesis of liver fluke-associated cholangiocarcinoma contributes to tumor growth and metastasis. | microrna, an endogenous noncoding rna modulating gene expression, is a key molecule that by its dysregulation plays roles in inflammatory-driven carcinogenesis. this study aimed to investigate the role of oncomir mir-21 and its target, the programmed cell death 4 (pdcd4) in tumor growth and metastasis of the liver fluke opisthorchis viverrini-associated cholangiocarcinoma (cca). the expression levels of mir-21 and pdcd4 were analyzed using the taqman mirna expression assay and immunohistochemist ... | 2013 | 23417858 |
| opisthorchis viverrini: implications of the systematics of first intermediate hosts, bithynia snail species in thailand and lao pdr. | bithynia snails are important hosts of the human liver fluke, opisthorchis viverrini. allozyme markers were used to examine genetic variation and the systematics of bithynia snails from different geographical localities in thailand and lao pdr. our results show that bithynia siamensis goniomphalos contains two major evolutionary clades with at least nine morphologically similar but genetically distinct "cryptic" species (taxa). clade a consists of snails from the chi and the mun river wetlands, ... | 2013 | 23305887 |
| prevalence of opisthorchis viverrini infection in nakhon ratchasima province, northeast thailand. | opisthorchis viverrini infection is a serious public-health problem in southeast asia especially in lao pdr and thailand. it is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the major etiology of cholangiocarcinoma. | 2012 | 23244144 |
| stage-specific expression and antigenicity of glycoprotein glycans isolated from the human liver fluke, opisthorchis viverrini. | infection by opisthorchis viverrini (liver fluke) is a major public health problem in southeastern asia, resulting in hepatobiliary disease and cholangiocarcinoma. fluke surface glycoconjugates are prominently presented to the host, thereby constituting a crucial immunological interface that can determine the parasite's success in establishing infection. therefore, n- and o-linked glycoprotein glycan profiles of the infective metacercarial stage and of the mature adult were investigated by nanos ... | 2013 | 23174105 |
| community-based cross-sectional study of carcinogenic human liver fluke in elderly from surin province, thailand. | opisthorchis viverrini infection is a serious public-health problem in southeast asia. it is associated with a number of hepatobiliary diseases and the evidence strongly indicates that liver fluke infection is the etiology of cholangiocarcinoma. | 2012 | 23167329 |
| species-specific primers designed from rapd products for bithynia funiculata, the first intermediate host of liver fluke, opisthorchis viverrini, in north thailand. | bithynia funiculata is the first intermediate host of the human liver fluke opisthorchis viverrini in northern thailand but its identification through morphological analysis is often problematic due to the shortage of gastropod taxonomists. as a consequence, we focused on the development of species-specific primers for use as an identification tool. our work involved recovery of a 502-base pair (bp) amplicon of unknown function through species-specific primers whose effectiveness was tested by a ... | 2013 | 23157281 |
| prkar1a overexpression is associated with increased ecpka autoantibody in liver fluke-associated cholangiocarcinoma: application for assessment of the risk group. | cholangiocarcinoma (cca) associated with opisthorchis viverrini (ov) chronic infection is the most frequent primary liver cancer in thailand, and current approaches to early diagnosis and curative treatments are largely disappointing. we hypothesize a role for protein kinase a (pka) in ov-induced cca. first, we studied the pka isozyme switching in the liver from the hamster cca model using quantitative (q) pcr, in situ hybridization, and immunohistochemical and western blot analysis. second, the ... | 2012 | 22922884 |