| roles of target cell membrane carbohydrate and lipid in entamoeba histolytica interaction with mammalian cells. | latex beads and liposomes carrying glycoproteins with carbohydrate sequences recognized by an entamoeba histolytica galactose-specific binding protein were assessed for their ability to adhere to trophozoites and to stimulate amoeba actin polymerization. glycoprotein-conjugated beads bound significantly to amoebae but did not stimulate actin polymerization. glycoprotein-bearing liposomes bound to amoebae and did enhance actin polymerization, as do recognized glycosphingolipid-bearing liposomes ( ... | 1990 | 1694827 |
| cloning and expression of a membrane antigen of entamoeba histolytica possessing multiple tandem repeats. | entamoeba histolytica causes amebic dysentery and amebic liver abscess, major causes of morbidity and mortality worldwide. we have used differential hybridization screening to isolate an e. histolytica-specific cdna clone. the cdna was found to encode a serine-rich e. histolytica protein (srehp) containing multiple tandem repeats. the structural motif of srehp resembles some of the repetitive antigens of malarial species, especially the circumsporozoite proteins. a recombinant trpe fusion protei ... | 1990 | 1695007 |
| characterization of an immuno-dominant variable surface antigen from pathogenic and nonpathogenic entamoeba histolytica. | a 125-kd surface antigen of entamoeba histolytica is recognized by 73% of immune sera from patients with amoebic liver abscesses. using pooled human immune sera a cdna clone (lambda cm17) encoding this antigen (m17) has been isolated from a lambda gt11 expression library of the virulent stain e. histolytica hm1:imss. monospecific antibodies, purified by binding to phage lysate of lambda cm17, and mab fa7 reacted exclusively with the 125-kd antigen by western blot analysis. surface binding and ca ... | 1990 | 1696956 |
| characterization of a monoclonal antibody that selectively recognizes a subset of entamoeba histolytica isolates. | monoclonal antibody 2d7.10 recognized an antigen present in seven of nine isolates of axenically cultured entamoeba histolytica and absent in all other entamoeba isolates studied. the antigen was absent in two isolates: 200:nih and rahman. all nine isolates belonged to pathogenic zymodeme ii. western blot (immunoblot) analysis and treatment with periodate and the proteolytic enzyme trypsin suggest that the antigen recognized by 2d7.10 is a carbohydrate moiety. | 1990 | 1698182 |
| detection of igg, iga, igm and ige antibodies in invasive amoebiasis in endemic areas. | entamoeba histolytica-specific serum igg, iga, igm and ige antibodies were assayed in cases of amoebiasis in an endemic area. patient groups consisted of amoebic liver abscess (n = 18), preabscess hepatic amoebiasis (n = 22) and amoebic colitis (n = 30). control subjects comprised 26 asymptomatic cyst passers, 13 giardiasis cases, 20 typhoid patients and 24 non-amoebic individuals. serum igg was assayed by elisa, using a monoclonal anti igg beta-galactosidase (igg beta-gal) conjugate, a polyclon ... | 1990 | 1699266 |
| [cross-reacting antigens of amebas and rat intestinal mucosa]. | the experiments have shown the presence of cross-reacting systems in entamoeba histolytica and in intestinal tissues of random noninbred albino rats. the antigenic affinity between e. histolytica and e. moshkovskii was expressed very weakly. | 1990 | 1705308 |
| homologous cysteine proteinases of pathogenic and nonpathogenic entamoeba histolytica. differences in structure and expression. | a cdna clone derived from the gene encoding a cysteine proteinase of pathogenic entamoeba histolytica was isolated using an antiserum to the purified enzyme. this clone was used to identify the homologous clone in a cdna library from nonpathogenic e. histolytica. sequence analysis and comparison of the predicted amino acid sequences revealed a sequence divergence of 16%. southern blot analyses indicated that (i) pathogenic isolates may contain more genes coding for these or related enzymes than ... | 1991 | 1705935 |
| specificity of commercially available entamoeba histolytica antigen in enzyme-linked immunosorbent assay (elisa) to sera from dogs with various parasitic infections. | | 1991 | 1718445 |
| reactivity of monoclonal antibodies to species-specific antigens of entamoeba histolytica. | twenty monoclonal antibodies were produced against trophozoites of entamoeba histolytica strains hk-9 and hm-1: imss. when reactivity to various enteric protozoa was examined by an indirect fluorescence antibody test, 15 of the monoclonal antibodies were strongly reactive with e. histolytica trophozoites. species-specific antigens recognized by these monoclonal antibodies were located on the plasma membrane, nucleus, cytoplasm, and cytoskeletal structures of the trophozoites. two of the remainin ... | 1991 | 1724012 |
| laboratory diagnosis of amebiasis. | the procedure used to diagnose amebiasis varies depending on the geographic location of the laboratory. however, in general, the microscopic diagnosis and differentiation of e. histolytica from other intestinal amebae is most satisfactorily achieved when the specimen is preserved immediately and both a concentration and stained smear are examined. the number of additional positive findings achieved by the cultivation of fecal material does not justify the time and cost involved. on the other han ... | 1991 | 1724953 |
| [diagnostic certainty in intestinal amebiasis in managua]. | three surveys were carried out in managua. nicaragua. in the first one it was found that 71% of the intestinal amebiasis cases of obligatory notification had no comparable diagnostic foundations; in the second one, 11.7% of the laboratory diagnoses as entamoeba histolytica referred by 4 health centers were correct; and in the third one, it was obvious that only 9.4% of the doctors knew well what intestinal amebiasis is. the results suggest that intestinal amebiasis is being diagnosed excessively ... | 1991 | 1725928 |
| [role of leukocytes and amebic proteases in experimental testicular necrosis produced in the rat by entamoeba histolytica]. | we examined the participation of polymorphonuclear leucocytes and amebic proteinases upon tissue damage by means of an experimental model of acute amebic lesions developed in rat's testicle. in leukopenic rats (less than 1,000 leucocytes/ml) intratesticular injection of axenic e. histolytica's trophozoites (hm-1) produced lesions undistinguishable from the normal controls. on the other hand, inhibition of 80% (average) of the proteinase activity by means of previous incubation of the trophozoite ... | 1990 | 1726660 |
| [obtaining monoclonal antibodies against outer membrane glycoproteins of entamoeba histolytica]. | the goal of this paper was the production of monoclonal antibodies capable of detecting relevant antigens from the surface of entamoeba histolytica trophozoites, with the purpose of using them as a diagnostic test. the cellular fusion for obtaining the monoclonal antibodies (mab) was done with spleen cells from balb/c mice, previously immunized with glycoproteins from the membrane, as well as sp2/0 cells. the hybridoma supernatants were tested with elisa, using glycoproteins and lipopeptide phos ... | 1990 | 1726661 |
| [the eosinophil and entamoeba histolytica. ii. testicular amebic lesions produced in eosinophilic rats]. | even though eosinophiles are not characteristic of amebiasis, polymorphonuclear eosinophilic cells are regularly found in the inflammatory lesion which occurs in the early phases of amebic invasion. to acquire better knowledge of the intervention of these cells when confronted with e. histolytica, testicular lesions were produced by means of direct injection of amebas in rats with eosinophilia. eosinophilia (greater than or equal to 4%) was induced in male sprague dawley rats by an intravenous i ... | 1990 | 1726663 |
| use of non-cellular models to study the interaction of e. histolytica with mammalian cells. | we have utilized liposomes constructed with individual mammalian cell membrane glycosphingolipids and latex beads conjugated with glycoproteins as models to investigate the molecular specificity and mechanism of interaction of e. histolytica with target cells. synthetic liposomes constructed with a variety of glycosphingolipids bearing neutral, straight chain glycans with galactose or n-acetylgalactosamine termini stimulated rapid (90 sec), contact dependent polymerization of e. histolytica acti ... | 1990 | 1726664 |
| molecular and cellular characterization of the 29-kilodalton peripheral membrane protein of entamoeba histolytica: differentiation between pathogenic and nonpathogenic isolates. | to further characterize the 29-kda surface antigen of entamoeba histolytica, we analyzed the complete nucleotide sequence and compared the immunoreactivity of this antigen in pathogenic and nonpathogenic strains. five cdna clones (one 1.0-kb full-length clone, designated p13, and four partial-length clones) encoding the antigen were analyzed for allelic variation. comparison of the nucleotide sequences revealed several single-nucleotide substitutions in all five cdnas, two of which resulted in a ... | 1992 | 1730488 |
| outcome of hepatic amebic abscesses managed with three different therapeutic strategies. | three different approaches to hepatic abscesses due to entamoeba histolytica were compared in 51 patients. the three modes of therapy utilized were: medical therapy with nitroimidazoles (n = 11 patients), open surgical drainage (n = 9 patients), and percutaneous drainage using ultrasound guidance followed by intralesional nitroimidazole administration (n = 31 patients). the results with each form of therapy were assessed clinically and by abdominal ultrasound. patients receiving combined us-guid ... | 1992 | 1735342 |
| sexually transmitted protozoal infections. trichomonas vaginalis, entamoeba histolytica, and giardia lamblia. | collectively, protozoa account for the largest number of stds worldwide. although these organisms can be eradicated effectively in the individual patient, their high asymptomatic carriage rate appears to have a significant influence on their continued transmission. the best hope for eradication of these organisms lies in a high index of suspicion in high-risk populations and the careful evaluation and treatment of sexual partners of infected persons. | 1992 | 1736479 |
| isolation and characterization of genomic clones encoding a serine-rich entamoeba histolytica protein. | | 1992 | 1741023 |
| enhancement of virulence of entamoeba histolytica by in vitro liver treatment. | enhancement of the virulence of five strains of entamoeba histolytica (three xenically maintained and two axenically maintained) was studied after in vitro incubation with normal hamster liver. increased virulence was shown by the ability of a small number of liver-treated trophozoites to produce liver lesions in hamsters. enhancement of virulence was positively correlated with increased resistance to normal hamster serum complement in vitro. | 1991 | 1746984 |
| an improved pvc strip ml-elisa technique for diagnosis of recent cases of amoebiasis. | conventional plate ml-elisa technique for diagnosis of recent cases of amoebiasis was converted into a simple polyvinyl chloride strip multilayer-enzyme linked immunosorbent assay (pvc strip ml-elisa). this report details the development and preliminary characterization of the pvc strip ml-elisa method for entamoeba histolytica antigen detection in human fecal samples. in addition, we evaluated the relative efficacies of the pvc strip ml-elisa and the conventional plate ml-elisa methods by scree ... | 1991 | 1757120 |
| gastroenterological emergencies in the tropics. | significant differences exist in the prevalence of most gastroenterological emergencies in tropical compared with temperate countries. both ethnic and environmental (often clearly defined geographically) factors are relevant. the major oesophageal lesions which can present acutely in tropical countries are varices and carcinoma; bleeding and obstruction are important sequelae. peptic ulcer disease (and its complications), often associated (not necessarily causally) with helicobacter pylori infec ... | 1991 | 1764626 |
| the entamoeba histolytica rdna episome: nuclear localization, dnaase i sensitivity map, and specific dna-protein interactions. | structural and functional features of the extrachromosomal dna element that contains the ribosomal rna genes of entamoeba histolytica were studied using a variety of techniques. using in situ hybridization, the element was found to be distributed along the inner phase of the nuclear membrane in the trophozoite stage; it appears to be part of the so-called peripheral chromatin. dnaase i-sensitive regions on the episome were mapped and found to correspond to the borders of the ribosomal rna coding ... | 1991 | 1766368 |
| amebiasis: clinical and laboratory perspectives. | entamoeba histolytica, the premier intestinal protozoan, has traversed time in its relentless quest for survival in its dichotomous role of parasite and pathogen. enigmatic in its transition from human intestinal commensal to invader of human tissue, diverse in its pathogenicity for the human host, and intricate in its bacterial interrelationship in the bowel, e. histolytica has become the focal point of intensive investigation in its basic biology underscoring human pathogenicity. this review w ... | 1991 | 1772585 |
| pathogenic and nonpathogenic entamoeba histolytica: identification and molecular cloning of an iron-containing superoxide dismutase. | superoxide dismutase (sod) activity was determined in the cell lysate of the axenically cultured entamoeba histolytica isolate hm-1:imss. under anaerobic culture conditions, 18.7 (+/- 4.9) units sod activity (mg protein)-1 were found. by inhibition studies the activity was attributed to an iron-containing type of sod (fesod). using degenerate oligonucleotide primers derived from regions highly conserved in prokaryotic fesod sequences, a genomic dna fragment was amplified by the polymerase chain ... | 1991 | 1775159 |
| antiamoebic and antiplasmodial activities of alkaloids isolated from strychnos usambarensis. | seven alkaloids isolated from strychnos usambarensis have been assessed for in vitro activities against entamoeba histolytica and plasmodium falciparum and for in vivo activity against plasmodium berghei in mice. strychnopentamine and 3',4'-dihydrousambarensine were highly active against p. falciparum in vitro, but were inactive and non-toxic against p. berghei in vivo. usambarensine, usambarine, and 18,19-dihydrousambarine were highly active against e. histolytica in vitro, but were less active ... | 1991 | 1775573 |
| use of patients' sera for immunoperoxidase demonstration of infectious agents in paraffin sections. | using patients' sera diluted from 1:10 to 1:1,000 as the primary antibodies in indirect immunoperoxidase staining, the authors visualized a variety of infectious agents in formalin-fixed, paraffin-embedded tissue sections. the target lesions included 1) pyoderma caused by staphylococcus aureus, 2) cryptococcal infection, 3) dermal sporotrichosis, 4) colon ulcer caused by amebic dysentery, 5) cutaneous leishmaniasis, and 6) chronic liver abscess containing ova of ascaris lumbricoides. the infecti ... | 1991 | 1776466 |
| monoclonal antibodies which inhibit in vitro cytotoxicity of axenic entamoeba histolytica. | a panel of 12 independent hybridoma cell lines secreting monoclonal antibodies to axenic e. histolytica (hm1) have been developed. a hybridoma cell line p4 c4 p2 f8 c8 (clone c8) produced monoclonal antibodies (moab c8) of igg1 isotype which recognised a 29 kd surface associated antigen of amoebic trophozoites in western immunoblot. immunofluorescent probing with moab c8 employing live and acetone fixed amoebic trophozoites indicated 29 kd molecule on the surface plasma membrane of e. histolytic ... | 1991 | 1778619 |
| pathogenesis of acute experimental amebic liver abscess in hamsters. | the hypothesis was tested that tissue necrosis in acute experimental amebic liver abscess in hamsters is not caused directly by the parasite but rather, indirectly, by the destruction of closely surrounding leukocytes that release their lysosomal enzymes and damage neighboring liver cells. axenically grown trophozoites of entamoeba histolytica strain hm-1 were injected intraportally into normal, hypocomplementemic, and leukopenic hamsters, and the lesions were studied histologically 2, 5, 10, an ... | 1991 | 1779303 |
| results of testing for intestinal parasites by state diagnostic laboratories, united states, 1987. | we analyzed results of 216,275 stool specimens examined by the state diagnostic laboratories in 1987; parasites were found in 20.1%. percentages were highest for protozoans: giardia lamblia (7.2%), entamoeba coli and endolimax nana (4.2% each), blastocystis hominis (2.6%), entamoeba histolytica (0.9%), and cryptosporidium species (0.2%). identifications of giardia lamblia increased broadly from the 4.0% average found in 1979, with 40 states reporting increases and seven decreases. most states th ... | 1991 | 1779956 |
| entamoeba histolytica and airline personnel. | | 1991 | 1781018 |
| in vitro study of different antiamoebic drugs. | | 1991 | 1788314 |
| in vitro evaluation of gossypol as potential antiamoebic drug. | | 1991 | 1788315 |
| [new aspects of the biology of entamoeba histolytica]. | to determine the mechanism of mitosis in e. histolytica, a study was carried out with axenic cultures of trophozoites in coplin jars with glass slides to stain them in situ with giemsa and fluorescent antibodies for actin. tobuline and myosin. sedimented amoeba of cultures were used also for ultrastructural studies after glutaraldehyde fixation, and inclusion in spon-araldite. giemsa stained trophozoites showed duplication by budding or fission with production of polykaryons, mononuclei, micronu ... | 1991 | 1790856 |
| down-regulation of murine lymphocyte responsiveness to mitogens after treatment with antigens of entamoeba histolytica. | injection of mice with pathogenic entamoeba histolytica (strain hm1-imss) antigens resulted in a decreased capacity of splenocytes to respond to mitogen-induced blastogenesis following a challenge with concanavalin a (con a), phytohemagglutinin (pha), and lipopolysaccharide (lps), whereas no inhibition was observed in mice that had previously been injected with equivalent amounts of non-pathogenic e. histolytica-like laredo antigens. depletion of adherent cells in the splenocyte preparation indi ... | 1991 | 1792226 |
| the plasma membrane of entamoeba histolytica: structure and dynamics. | the plasma membrane components of the parasitic protozoan entamoeba histolytica, the causative agent of human invasive amebiasis, have been biochemically and immunologically characterized during the last decade. in addition, genes coding for certain surface proteins have been cloned. in spite of these advances, a unified characterization of plasma membrane antigenic components of the parasite is still required for badly needed advancements in the design of useful diagnostic, epidemiologic, and i ... | 1991 | 1794060 |
| [intestinal parasites in primary school students]. | five hundred thirty-one stool samples obtained from primary schools. the age range was between 7 and 13 years, 49% of the patients were females an 51% of the patients males. in our study it has been observed that the parasite incidence was 36.9% the predominant parasite was ascaris lumbricoides (12%), giardia intestinalis (9%), hymenolepis nana (7.1%) entamoeba histolytica (6%) and trichuris trichiura (2.6%) respectively, we detected six different parasites in the primary school students because ... | 1991 | 1795661 |
| in vitro effects of berberine sulphate on the growth and structure of entamoeba histolytica, giardia lamblia and trichomonas vaginalis. | the plant alkaloid, berberine sulphate, inhibited the growth of entamoeba histolytica, giardia lamblia and trichomonas vaginalis in bi-s-33 medium, and induced morphological changes in the parasites. exposure of e. histolytica to berberine caused a clumping of chromatin in the nucleus, and the formation of autophagic vacuoles and aggregates of small vacuoles in the cytoplasm. in berberine-treated g. lamblia, an irregularly-shaped vacuole appeared in the cytoplasm and gradually enlarged during cu ... | 1991 | 1796883 |
| a study of the bacterial and parasitic causes of acute diarrhoea in northern jordan. | the prevalence of bacterial and parasitic diarrhoeagenic agents in 200 patients suffering from acute diarrhoea and reporting to hospitals and health centres in northern jordan, and in controls was determined. one or more bacterial or parasitic enteropathogens was isolated from 79 patients (39.5%). prevalence rates for these pathogens was as follows: enterotoxigenic escherichia coli, 9%; enteropathogenic e. coli, 9%; salmonella spp. 7%; campylobacter spp, 5.5%; yersinia enterocolitica, 4.5%; shig ... | 1991 | 1800560 |
| inhibition of axenically grown entamoeba histolytica by purine nucleoside analogs and actions of natural nucleosides. | the effects of the nucleoside analogs tubercidin, nebularin, formycin b and 3'-deoxyinosine on axenically grown entamoeba histolytica were tested. both tubercidin and nebularin showed pronounced inhibitory action, 50% of growth inhibition (ic50) being obtained at 0.14 and 0.82 microm, respectively. formycin b and 3'-deoxyinosine were essentially inactive or weakly active at concentrations above 10 microm. natural nucleosides, including adenosine, inosine, guanosine, thymidine, uridine and cytidi ... | 1991 | 1801136 |
| a modification of diamond's medium for the axenic culture of entamoeba histolytica. | the use of casein hydrolysate in diamond's axenic culture medium tps-1 in replacement of trypticase allowed good growth of the trophozoites of entamoeba histolytica. this modified medium also supported growth of trophozoites preserved for 16 months in liquid nitrogen. considerable labour and cost of serum can be saved by using 5% instead of 10% bovine serum in combination with this modified medium. | 1991 | 1801343 |
| enhanced antiparasitic activity of lipophilic tetracyclines: role of uptake. | it was previously noted that the inhibitory activities of lipophilic tetracyclines against the growth of giardia lamblia in vitro were up to 40-fold greater than those of nonlipophilic tetracyclines (50% inhibitory concentration [ic50] = 1.8 to 71 micrograms/ml) (t. d. edlind, antimicrob. agents chemother. 33:2144-2145, 1989). we have now extended this observation to trichomonas vaginalis (ic50 = 2.9 to 200 micrograms/ml), entaoeba histolytica (ic50 = 3.8 to 36 micrograms/ml), and leishmania maj ... | 1991 | 1803991 |
| phagocytic capacity of kupffer cells during hepatic amoebiasis in guinea pigs. | very few studies have been carried out on the role of liver macrophages (kupffer cells) during the course of hepatic amoebiasis. the kinetics of phagocytic activity of kupffer cells and blood monocytes was studied in guinea pigs intra-mesenterically infested with entamoeba histolytica. the phagocytic capacity of blood monocytes of normal animals was comparatively lower than kupffer cells for both latex and haemolysin coated sheep red blood cells. significant decline in phagocytic response of kup ... | 1991 | 1804871 |
| autochthonous amoebiasis in institutionalized mentally-retarded patients: preliminary evaluation of isoenzyme patterns in three isolates. | three autochthonous cases of entamoeba histolytica infection in institutionalized mentally-retarded patients are reported. isoenzyme analysis by starch-gel electrophoresis shows the pathogenicity of the three isolates: two belong to zymodeme ii, and one to zymodeme xix. the study shows that invasive e. histolytica strains occur in italy and can be isolated from institutionalized oligophrenic patients. | 1991 | 1809244 |
| [isoenzymatic characterization of strains of entamoeba histolytica isolated from cuban patients and foreigners from africa]. | an analysis was made of the isoenzyme patterns of 61 entamoeba histolytica strains: 42 from cuba and 19 from angola mozambique and ghana. by means of these patterns the non-pathogenic nature of the cuban strains (group 1) was shown. it coincided with the clinical characteristics of the infection in the carriers. among the imported strains were 2 samples with pathogenic patterns ii corresponding to 1 dysentery case and to 1 asymptomatic patient, and also non-pathogenic ones (i, ii, iii and xviii) ... | 1991 | 1812535 |
| protective immunity to entamoeba histolytica infection in subjects with antiamoebic antibodies residing in a hyperendemic zone. | to investigate the relationship between amoebic gut infection and the presence of serum antiamoebic antibodies in a community where amoebiasis is highly endemic, a cross-sectional population survey was conducted covering 982 subjects in 2 adjacent villages in rural india. e. histolytica was detected in fecal smears in 18.1% (88/487) and antiamoebic antibodies by micro-elisa in 28.7% (251/874) subjects. in 450 subjects in whom there was no clinical evidence of invasive amoebic disease or history ... | 1991 | 1815341 |
| synthesis and study of antiprotozoal activity of some 5-nitrothiophene aldimines. | this paper reports on the synthesis and the antiparasitical activity of some 5-nitrothiophene aldimines variously substituted on aniline nucleus. the influence of substituents on the activity of the prepared compounds is discussed. | 1991 | 1815581 |
| detergent dissection of membrane proteins of entamoeba histolytica and its effect on lymphokine release in in vitro. | fifty-two amoebic liver abscess cases were assessed for the release of lymphokines (lmif) using detergent dissected membrane proteins (ddmp) of axenic entamoeba histolytica (nih:200) obtained with sodium deoxycholate treatment. lymphokines release by t lymphocytes in response to both ddmp and whole amoebic lysate (wal) was tested by leukocyte migration inhibition test on blood samples from amoebic liver abscess cases. a significant increase was noted in the release of lmif and 100% positivity wa ... | 1991 | 1816085 |
| [3 cases of hematogenous lung abscess of amebic origin]. | invasive amebiasis is a very serious health problem in mexico as it is presumably related to the presence of virulent strains of entamoeba histolytica and poor hygienic and sanitary conditions; other factors related to invasive amebiasis are undernutrition, alcoholism, and homosexuality. we present three patients with pulmonary amebic hematogenous abscess. clinically all patients had the typical "chocolate" exudate. the three patients had pulmonary consolidations by chest roentgenogram; one of t ... | 1991 | 1818373 |
| invasive amebiasis in a spider monkey (ateles geoffroyi). case report and a short review of the literature of amebiasis in non-human primates. | a spider monkey (ateles geoffroyi) studied at the san juan de aragon zoo died with symptoms of amoebic dysentery verified by coprologic investigation of the parasite, his female partner was cured with conventional antiamoebic treatment. at autopsy widespread ulcerations in the colon and multiple liver abscesses were found, containing trophozoites of entamoeba histolytica. both lesions were identical to those observed in human amoebiasis. a short review of the literature in nonhuman primates infe ... | 1991 | 1819979 |
| seroepidemiology of anti-entamoeba histolytica antibody by enzyme-linked immunosorbent assay in the greater tokyo area. | an enzyme-linked immunosorbent assay (elisa) for detecting antibody against entamoeba histolytica was used in a survey to determine the prevalence of e. histolytica infection in the greater tokyo area. serum samples were collected from 1,869 randomly chosen, symptom-free volunteers (1,645 males and 224 females). seventeen individuals (0.91%), 14 males and three females, had elisa values that were interpreted as positive. the calculated histogram of the anti-e. histolytica igg values, after logar ... | 1991 | 1820666 |
| experimental fusion of e. histolytica cells by horseradish peroxidase treatment. | the experimental fusion of entamoeba histolytica cells after their treatment with horseradish peroxidase is reported. this finding is discussed in relation with the general knowledge about the cell fusion process. | 1991 | 1821166 |
| cytopathogenicity of entamoeba histolytica to human intestinal epithelial cells: inhibition by monoclonal antibodies and sera from amoebic patients. | interactions of pathogenic entamoeba histolytica (hm 1) with human intestinal epithelial cells (henle-407) were investigated. the e. histolytica trophozoites adhered and cytolysed 87% of cultured epithelial cell monolayers. a significant (p less than 0.001) inhibition of cytopathic effect of amoebic trophozoites pretreated with monoclonal antibodies to a 29 kda surface associated protein suggested utilization of the 29 kda surface protein in recognition and cytolysis of epithelial target cells. ... | 1991 | 1821426 |
| entamoeba histolytica zymodemes: exhibition of gamma and delta bands only of glucose phosphate isomerase and phosphoglucomutase may be influenced by starch content in the medium. | entamoeba histolytica isolated from human can be associated with either symptomatic disease or with asymptomatic carriers. pathogenic and nonpathogenic strains can be distinguished on the basis of differences in the electrophoretic patterns of four isoenzymes (zymodeme). with glucose phosphate isomerase and phosphoglucomutase, we observed variation of the expression of their gamma bands as a function of starch volume in culture. we cultured e. histolytica strains from different zymodemes in robi ... | 1991 | 1825198 |
| [distribution of zymodemes of entamoeba histolytica from different areas of china]. | using agarose gel electrophoresis, the electrophoretic patterns of four enzymes: malate nadp oxidoreductase (malic enzyme) (me), glucosephosphate isomerase (gpi), phosphoglucomutase (pgm) and hexokinase (hk), were compared and analysed for five strains of entamoeba histolytica, which had been obtained from patients with acute amebic dysentery or asymptomatic cyst carrier in beijing, tianjin and fujian province. the results showed that the electrophoretic patterns of all the five strains of e. hi ... | 1991 | 1829658 |
| [prevalence of intestinal parasitoses in a sample of italian and immigrant workers employed in the food sector of turin]. | we studied the prevalence of intestinal parasites in a random sample of immigrants and italians employed in the food industry. prevalence ranged from 20.8% among immigrants to 5.6% among italians. where helminths are widespread among indochinese, protozoa are more common among africans. three cases of entamoeba histolytica in asymptomatic subjects were identified and successfully treated. we observed high levels of giardia intestinalis both in italians (2.2%) and immigrants (3.9%). prevalence of ... | 1991 | 1838507 |
| [immune response induced by phosphofructokinase from e. histolytica in hamsters]. | the enzymatic activity of inorganic pyrophosphate (ppi) dependent phosphofructokinase became manifest in the supernatant obtained by centrifugation in a homogenate of e. histolytica strain hmi-imss at 700,000 g. partial purification of the enzyme was achieved by column chromatography with ultrogel aca-34. ten protein elution spikes were obtained: five showed enzymatic activity. elution spikes i and ii attained the highest values of specific enzymatic activity 6.45 and 6.98 u/mg of protein, respe ... | 1991 | 1840160 |
| immunogenicity of detergent membrane proteins of entamoeba histolytica. | the lymphokine release and antibody production were assessed in the peripheral blood of 52 and 48 cases of amoebic liver abscess respectively, by employing detergent dissected membrane proteins (ddmp) of axenic entamoeba histolytica (nih:200). lymphokine release by t lymphocytes in response to both ddmp and whole amoebic lysate (wal) was performed by leukocyte migration inhibition test. a highly increased release of lmif and 100 per cent positivity was observed with ddmp where as the same for wh ... | 1991 | 1841452 |
| entamoeba histolytica: antigenic characterization of axenic strains from brazil. | trophozoites from cultures of entamoeba histolytica strains isolated and grown axenically in brazil (icb-csp, icb-462 and icb-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in usa (hk-9). hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. the four strains in study revealed sim ... | 1991 | 1843399 |
| dot-enzyme-linked immunosorbent assay (dot-elisa) of anti-entamoeba histolytica antibodies in human serum and colostrum. | seroepidemiologic and clinical studies of amoebiasis require improved methods for the detection and titration of anti-entamoeba histolytica (eh) trophozoite antibodies. we have developed a dot-enzyme-linked immunosorbent assay (dot-elisa) to titrate anti-eh antibodies in human serum and colostrum. the anti-eh serum igg antibody titers from healthy individuals and from patients with amoebic liver abscess obtained, with our dot-elisa were similar to those obtained with a standard elisa, but the ba ... | 1991 | 1844108 |
| health impact evaluation of wastewater use in mexico. | wastewater from mexico city is used to irrigate over 85 000 hectares, mainly of fodder and cereal crops in the mezquital valley. a cross-sectional study method is being used to test the impact of exposure to raw wastewater and wastewater from storage reservoirs on diarrhoeal disease and parasitic infections in farmworkers and their families. the study population in the rainy season survey included 1 900 households: 680 households where the farmworker is exposed to untreated wastewater (exposed g ... | 1991 | 1844273 |
| [blastocystis hominis in canavese: a retrospective study of samples received for fecal parasitological examination at the ivrea- castellamonte hospital over 42 months]. | in the last ten years the interest in the controversial blastocystis hominis microorganism of the intestine has greatly increased. the authors conducted a retrospective study of feces for parasitology investigations at the ivrea-castellamonte hospital. these investigations lasted 42 months (1/1/1988-31/6/1991) and the authors focused their attention on samples with 5 or more blastocystis hominis at the microscopically examination with a 40x phase objective. during the study 2,138 samples of fece ... | 1991 | 1844921 |
| antibodies against entamoeba histolytica in individuals with intestinal amoebiasis presenting cysts and/or trophozoites in the feces. | serum samples were obtained from 154 individuals infected with entamoeba histolytica (78 symptomatic and 76 asymptomatic). twelve had trophozoites in the feces whereas 142 had only cysts. the sera were used to test the existence of antibodies anti-entamoeba histolytica employing the indirect hemagglutination (iha), indirect immunofluorescence (ifat), complement fixation reaction (cfr) and counterimmunoelectrophoresis (ciep). for those individuals with trophozoites in their feces, 75.0 were posit ... | 1991 | 1844965 |
| crohn's colitis complicated by superimposed invasive amebic colitis. | the clinical characteristics and endoscopic appearance of inflammatory bowel disease (ibd) may be very similar to those of amebic colitis. physicians, especially in areas in which amebiasis is endemic, are familiar with this difficulty. moreover, in individual cases, it may even be impossible to distinguish between the two conditions, since stool specimens, bowel biopsies, and serological studies may be negative for entamoeba histolytica, even in the presence of invasive amebic colitis. invasive ... | 1991 | 1858742 |
| variations in cytotoxicity and isoenzyme patterns of uncloned and cloned cultures of axenic entamoeba histolytica. | five clones of axenic entamoeba histolytica (hmi) grown as discrete colonies in semisolid agar medium were adapted in liquid medium and labelled as hmi-c121, hmi-c131, hmi-c143, hmi-c144 and hmi-c145. the clone hmi-c121 was more cytotoxic to the cultured baby hamster kidney (bhk) cells while all other clones were significantly (p less than 0.001) less cytotoxic as compared to the cloned hmi-c121 and uncloned e. histolytica (hmi). the uncloned indian axenic e. histolytica (kcg:0986:11) as well as ... | 1991 | 1863471 |
| [preparation of oxime, hydrazone and dichloroacetamide derivatives. researches of antiparasitic activity]. | several oximes, hydrazones and dichloroacétamides were synthesized. the antiparasitic properties of these compounds were evaluated in vitro against two protozoaires entamoeba histolytica and trichomonas vaginalis. | 1991 | 1867460 |
| amoebic liver abscess. review of 220 cases. | two hundred and twenty cases of amoebic liver abscess were seen between 1981-1986. the majority were young or middle aged males belonging to the lower socio-economic group and 85% gave a history of drinking toddy (fermented palm juice). the highest incidence of the disease occurred during the peak toddy season (april to july). toddy drinkers become susceptible perhaps because of the large dose of entamoeba histolytica and bacteria ingested with the drink, associated malnutrition, poor hygiene, h ... | 1991 | 1869393 |
| study of entamoeba histolytica isolates from calcutta with special reference to their zymodeme, animal pathogenicity and erythrophagocytosis. | entamoeba histolytica isolates, obtained mainly from asymptomatic human cyst passers, were tested for their in vitro erythrophagocytic activity and lesion-forming ability in hamster liver. simultaneous zymodeme typing of the isolates showed that, along with the pathogenic zymodemes, a number of isolates having nonpathogenic zymodemes also produced liver lesions in hamsters and engulfed higher numbers of rbcs. the observed positive correlation between the rate of erythrophagocytosis of an e. hist ... | 1991 | 1869796 |
| a serum-free, partly defined medium, pdm-805, for axenic cultivation of entamoeba histolytica schaudinn, 1903 and other entamoeba. | we describe the first serum-free, partly defined medium (pdm-805) for cultivating the human enteric pathogen, entamoeba histolytica, and the reptilian amebae e. barreti, e. invadens, and e. terrapinae. pdm-805 was developed by the stepwise replacement of yeast extract, bovine serum, and a casein peptone digest in tyi-s-33, a medium widely used for the axenic cultivation of these parasites. the defined components include amino acids, carbohydrates, b vitamins, ascorbic acid, tocopherol, thioctic ... | 1991 | 1880759 |
| pore-forming peptide of pathogenic entamoeba histolytica. | a polypeptide that causes pore formation in target-cell membranes is implicated in the potent cytolytic activity of pathogenic entamoeba histolytica. pore-forming material was purified to apparent homogeneity by a multistep procedure, and its analysis by nadodso4/page revealed one peptide of 4-5 kda under nonreducing or under reducing conditions. pore-forming activity was measured by depolarization of liposome membrane potential and was found to be optimally expressed at low ph. active material ... | 1991 | 1881907 |
| characterization of 20 entamoeba histolytica strains isolated from patients with hiv infection. | twenty entamoeba histolytica strains from patients with hiv-1 infection were isolated and compared with e. histolytica strains from patients without hiv infection. the isoenzyme pattern of the hexokinase as well as the hybridization with known dna-probes were used as markers for pathogenicity. according to these markers, all 20 strains could be regarded as being nonpathogenic. direct measurements of the virulence were carried out: destruction of monolayer tissue culture cells, capacity of phagoc ... | 1991 | 1889871 |
| isolation of a strain-specific entamoeba histolytica cdna clone. | entamoeba histolytica is an intestinal parasite causing significant morbidity and mortality worldwide. more tools are needed to understand the epidemiology and molecular pathogenesis of amebiasis. a cdna library was constructed by using poly(a)+ rna isolated from an axenic strain of e. histolytica, hm1:imss, which expresses a pathogenic isoenzyme pattern (zymodeme). differential screening of the library yielded a strain-specific 3' polyadenylated cdna clone, c2, possessing nine 26-nucleotide tan ... | 1991 | 1890170 |
| molecular probe for identification of trichomonas vaginalis dna. | trichomoniasis is one of the most widespread sexually transmitted diseases in the world. diagnosis can be achieved by several methods, such as direct microscopic observation of vaginal discharge, cell culture, and immunological techniques. a 2.3-kb trichomonas vaginalis dna fragment present in strains from diverse geographic areas was cloned and used as a probe to detect t. vaginalis dna in vaginal discharge by a dot blot hybridization technique. this probe was specific for t. vaginalis dna. it ... | 1991 | 1890171 |
| entamoeba histolytica: a simplified method to quantify its cytotoxicity. | a simplified and reliable method to quantify entamoeba histolytica cytotoxicity was standardized. mice spleen leucocytes were utilized as target cells. interaction time was reduced to 1 h by pelleting interacting cells. to assess target-cell killing by amoebae, a nigrosine exclusion test was employed. fixation with glutaraldehyde stabilized the percentage of stained target cells. similar results were obtained when cytotoxicity of the e. histolytica hm1 strain was tested by the traditional and pr ... | 1991 | 1894436 |
| distinguishing pathogenic isolates of entamoeba histolytica by polymerase chain reaction. | | 1991 | 1894950 |
| serodiagnosis of invasive amebiasis using a recombinant entamoeba histolytica protein. | one hundred eight serum samples from 106 patients were examined by western blot analysis for the presence of antibodies to a recombinant fusion protein containing the sequence of the newly described serine-rich entamoeba histolytica protein (srehp). among patients with invasive amebiasis from durban, republic of south africa; san diego, calif; mexico city, mexico; and st louis, mo, 53 (82%) of 65 had antibodies to srehp. in contrast, only one patient (2%) of 43 without acute invasive amebiasis h ... | 1991 | 1895478 |
| hospital-acquired diarrhea in adults: a prospective case-controlled study in mexico. | to know the incidence, etiology, risk factors, morbidity, and mortality of nosocomial diarrhea in adults. | 1991 | 1906497 |
| a study of serum antibody levels against entamoeba histolytica in patients with different amoebic zymodemes. | the present study has looked into relative agreement between clinical diagnosis, indirect fluorescent antibody test and iso-enzyme grouping in 35 patients with e histolytica infection. thirty caucasians, who had never travelled to tropical countries were chosen as negative control subjects. there was no remarkable difference in the mean amoebic antibody levels observed with the control subjects and patients harbouring amoebic zymodeme i or iii or iv. whereas, comparative analysis revealed a sign ... | 1991 | 1911488 |
| entamoeba histolytica: microtubule movement during mitosis. | the movement of microtubules (mts) during nuclear division of entamoeba histolytica was ultrastructurally studied. regarding this mt movement, five stages of mitosis could be defined: prophase, metaphase, anaphase a, anaphase b, and telophase. in early stages of mitosis, chromatinic material appeared condensed, and mts were detected in the center of the nucleus. later, mts seemed to grow from an electron-dense body located in the center of the nucleus. this body might be the microtubule organizi ... | 1991 | 1915743 |
| amebic pericarditis following ruptured right liver lobe abscess. | we present an unusual case of suppurative pericarditis following rupture of a solitary right lobe amebic liver abscess. the condition was treated successfully by drainage of the liver abscess alone. | 1991 | 1916959 |
| biochemical and ultrastructural studies induced by phanquone on entamoeba histolytica nih-200. | the action of phanquone on the incorporation of (14c)1-dl-valine and (14c)-8-adenine in tca insoluble fractions of entamoeba histolytica was examined as indices of rna and protein synthesis respectively. the former was found to show greater sensitivity to the drug in terms of the effect manifested in the dose response curves for these parameters. electron microscopic investigations with increasing concentrations of the drug demonstrated progressive vacuolization with concomitant dissolution of r ... | 1991 | 1918871 |
| incidence of intestinal parasitic disease in an acquired immunodeficiency syndrome day-care center. | in june, 1986, the bronx municipal hospital center opened an acquired immunodeficiency syndrome day-care center to provide a quality educational experience for children infected with the human immunodeficiency virus. a major concern was the possibility of increasing secondary infections among these immunocompromised children by placing them in a group environment. one particular worry was intestinal parasitic disease, a serious public health problem in day-care centers throughout the united stat ... | 1991 | 1923677 |
| nucleotide sequence of a small subunit ribosomal rna (16s-like rrna) gene from entamoeba histolytica: differentiation of pathogenic from nonpathogenic isolates. | | 1991 | 1923831 |
| [synthesis and antiparasitic activity of various n-acetylacetates of nitrogen heterocycles]. | the synthesis and the study of the antiparasitic activity of various heterocyclic n-ethylacetates derived from substituted derivatives of piperazine, benzimidazole and benzotriazole do not show antiparasitic property. a slight activity on entamoeba histolytica and trichomonas vaginalis is noted with the nitro derivatives of benzimidazole and benzotriazole. | 1991 | 1929120 |
| isoenzymes in entamoeba as detected by isoelectrofocusing. | two major zymodemes from different entamoeba histolytica strains were detected, despite different growth conditions, using isoelectrofocusing in ultrathin gels. one was associated with strains cultured from symptomatic patients, while the other was associated with microorganisms obtained from clinically symptomatic persons. | 1991 | 1930579 |
| the d-galactose-inhibitable lectin of entamoeba histolytica. | | 1991 | 1933948 |
| dot immunobinding assay versus sandwich elisa in diagnosis of invasive amoebiasis. | sera from 34 patients of amoebic liver abscess (ala) and 11 patients with amoebic dysentery (ad) were examined for the presence of specific entamoeba histolytica (eh) antibodies and amoebic antigen by enzyme linked immunosorbent assay (elisa) and dot immunobinding assay (dib). both techniques were found to be equally sensitive for detecting antiamoebic antibodies (89.5 and 91.9% respectively) and highly specific (100%) in patients of ala. elisa was found to be more sensitive (94.4%) in detecting ... | 1991 | 1937593 |
| a new in vitro model of entamoeba histolytica adhesion, using the human colon carcinoma cell line caco-2: scanning electron microscopic study. | the human colon carcinoma cell line caco-2, which is widely used to study the adhesion and cytotoxicity of enterobacteria, was used to investigate the adhesion of the trophozoites of entamoeba histolytica. we observed a high percentage of adhesion of amoebae to caco-2 cells. scanning electron microscopy showed that amoebial membrane structures were involved in adhesion and the cytolytic action. these differentiated cells should prove to be a useful model system for investigation of the pathogeni ... | 1991 | 1937772 |
| role of the galactose lectin of entamoeba histolytica in adherence-dependent killing of mammalian cells. | entamoeba histolytica extracellular killing of host cells is contact dependent. adherence to human colonic epithelial cells and mucins is mediated by a galactose-specific lectin. the effect on cytotoxicity of a panel of monoclonal antibodies (mab) directed against the galactose lectin was tested. as expected, those mab which inhibited adherence also decreased cytotoxicity. however, one antilectin mab blocked cytotoxicity after adherence had occurred, indicating that the lectin has a role in cell ... | 1991 | 1937828 |
| differences in genomic dna sequences between pathogenic and nonpathogenic isolates of entamoeba histolytica identified by polymerase chain reaction. | a lambda gt11 cdna library was constructed from the poly(a)+ rna of trophozoites of entamoeba histolytica hm-1:imss strain. the library was immunologically screened with monoclonal antibody 4g6, which is specific for the 30,000-mr antigen of pathogenic isolates. a 0.7-kb clone was isolated, and its nucleotide sequence was determined. to examine whether this gene was specific for pathogenic isolates, a polymerase chain reaction was performed by using four sets of primers and the genomic dna of pa ... | 1991 | 1939576 |
| protein a-antibody mediated hemagglutination assay for serodiagnosis of amebic liver abscess. | a successful modification of the indirect hemagglutination test to demonstrate antibodies for serodiagnosis of amebic liver abscess has been described in the present study. in the modified test, the protein a-ima, staphylococcus aureus (cowan's strain i) bearing protein a (sapa) cells were used to enhance hemagglutination of sensitized red cells. use of sapa cells markedly enhanced sensitivity of the test and greatly increased the titers obtained with most of the sera. at a diagnostic antibody t ... | 1991 | 1948287 |
| pathogenicity and antigenic components of excysted giardia lamblia isolated from patients in riyadh, saudi arabia. | giardia lamblia cysts were isolated from patients in riyadh, saudi arabia. cysts and trophozoites (from axenically excysted cysts) were given orally by gavage to mice to establish the pathogenicity of the riyadh isolate. there was no effect of varying the dose of administered parasite on parasite excretion or morbidity. a typical pathologic pattern of giardiasis was demonstrated by histologic methods and electron microscopy. antigenic components of the riyadh isolate were compared with the portl ... | 1991 | 1951852 |
| intestinal parasitic infestation in pediatric gastroenterology outpatient clinic dr. pirngadi hospital medan. | a retrospective study had been done at the outpatient clinic sub division of pediatric gastroenterology, dr. pirngcdi hospital medan, comprising patients from 1 january 1985-31 december 1987. there were 874 patients. three hundred seventy eight (43.25%) patients consisting of 210 (55.56%) males and 168 (44.44%) females had intestinal parasitic infestations. the youngest patient was 5 months and the oldest was 14 years and 3 months. ascaris lumbricoides was found in 215 patients (24.60%) while tr ... | 1991 | 1956691 |
| factors associated with the detection of entamoeba histolytica in homosexual men. | in a study of bowel parasites in 128 australian homosexual men attending a sexually transmitted diseases (std) clinic, entamoeba histolytica was detected in 37%, giardia intestinalis in 3% and at least one protozoan in 81% of the group. there was no evidence of pathogenicity of e. histolytica, nor was there any association between the detection of e. histolytica and sexual practices, gastrointestinal symptoms, proctitis, human immunodeficiency virus antibody result or t-cell subset values. howev ... | 1991 | 1958719 |
| [experimental studies on the concurrent infections with entamoeba histolytica and schistosoma japonicum]. | the mongolian gerbil (merious unguiculatus) was used as an experimental animal model for studying the concurrent infections and pathogenicities with entamoeba histolytica (e. h.) and schistosoma japonicum (s. j.) by the cutaneous infection with 20 fresh cercariae of s. j. and the intracaecal inoculation of 5 x 10(5)/0.5 ml amebic suspension. light and electron microscopy were used to observe the pathological changes of the caecal wall in the concurrent infections. the results showed that s. j. i ... | 1991 | 1959165 |
| [monocyte locomotion inhibiting factor (mlif) produced by e. histolytica induces an increase of camp in human monocytes]. | the supernatant fluid of axenically grown e. histolytica contains a factor (mlif) which inhibits the locomotion of human monocytes (including chemotaxis) without affecting that of human polymorphonuclear leucocytes. locomotion, like other cellular functions, is modulated by changes in intracellular camp and cgmp. the consensus--with some exceptions--is that while rises in cgmp accompany locomotion, an increase in camp (without a concomitant fall in -cgmp) occurs with inhibition of cellular movem ... | 1990 | 1967028 |
| emetine-resistant mutants of entamoeba histolytica overexpress mrnas for multidrug resistance. | we report here the identification of multiple 344-bp segments of amoeba dna similar to the human multidrug resistance (mdr) gene using primers to conserved regions of the p-glycoprotein and the polymerase chain reaction (pcr). the amino acid sequences of amoeba mdr-like pcr products were 46-97% identical to each other, 46-51% identical to human mdr1 sequences, and 30-35% identical with plasmodium falciparum mdr-like sequences. on northern blots, the mdr-like pcr products identified amoeba mrnas ... | 1990 | 1970121 |
| emetine resistant mutants of entamoeba histolytica overexpress mrnas for multidrug resistance. | although drug therapy is critical for control of amoebiasis, little is known about mechanisms of drug resistance by e. histolytica parasites. here we tested the hypothesis that multidrug resistant (mdr) amoeba mutants, similar to mdr tumor cells, are drug resistant based upon overexpression of a p-glycoprotein pump that effluxes drugs from the cells. using primers to conserved regions of the human p-glycoprotein and the polymerase chain reaction (pcr), we identified multiple 344 base par segment ... | 1990 | 1983787 |
| protection of gerbils from amebic liver abscess by immunization with the galactose-specific adherence lectin of entamoeba histolytica. | no protective antigens from entamoeba histolytica have been previously defined. we tested the ability of the galactose-specific adherence lectin of e. histolytica to elicit a protective immune response in conjunction with freund's incomplete and complete adjuvants. the gerbil (meriones unguiculatus) model of an experimental amebic liver abscess was used. gerbils were immunized intraperitoneally or subcutaneously with 10 micrograms of the affinity-purified lectin in complete freund's adjuvant and ... | 1991 | 1987067 |
| intracranial amebic abscesses: ct and mr findings. | the ct and magnetic resonance findings in a case of intracranial amebic abscesses are described. due to increased travel to endemic areas, amebic abscess should also be included in the differential diagnosis for patients who live in the temperate parts of the world. | 1991 | 1987193 |