| trichomonas infections in men. | trichomonas vaginalis, a common pathogen in the female genital tract, produces a characteristic clinical picture in women. less well recognized are the manifestations of trichomonas infestations of the male genital tract, which include urethritis and chronic prostatitis. multiple-glass urinalysis and selective use of trichomonas cultures may improve recognition of this organism in the family practice setting. | 1989 | 2916396 |
| [current therapeutic aspects of trichomonas vaginalis infections in gynecology]. | | 1987 | 2972473 |
| presence of laminin-binding proteins in trichomonads and their role in adhesion. | adhesion is regarded as an important feature in the pathogenesis of various microorganisms. ability to recognize extracellular matrix proteins, such as laminin or fibronectin, has been correlated with invasiveness. we report that laminin enhances the adhesion of the parasitic protozoa trichomonas vaginalis and tritrichomonas foetus to a polystyrene substrate and to the surface of epithelial cells (madin-darby canine kidney cell line) in vitro. the enhancement was higher for t. vaginalis than for ... | 1988 | 2973059 |
| evolutionary conservation of laminin-binding proteins. | 1. the virulence of pathogens and metastatic capacity of cancer cells seems to correlate with the ability to adhere to cells and/or to basement membrane components. a key feature of this mechanism is the expression of specific receptors for the basement membrane protein laminin. three different receptors have been already described in cells phylogenetically very distant, such as human white blood cells, trichomonas vaginalis and staphylococcus aureus, all recognizing laminin with the same range ... | 1988 | 2977953 |
| the association of sexually transmitted diseases with cervical intraepithelial neoplasia: a case-control study. | thirty-three women with histologically confirmed cervical intraepithelial neoplasia (grades i to iii, with one case of microinvasive carcinoma) and 54 women without evidence of the disease were prospectively studied to determine the relationship of genital infection to cervical neoplasia. demographic and sexual data for patients and control subjects were collected, with standardized clinical and colposcopic evaluation by means of predefined diagnostic categories. cultures from the cervix were ex ... | 1985 | 2982264 |
| a linear double-stranded rna in trichomonas vaginalis. | a "double-stranded" rna was identified in the anaerobic, parasitic protozoan trichomonas vaginalis. electron microscopic evidence indicated linear double-stranded structure 1.5 micron in length, with no apparent hairpins or loops. boiling in 30% dimethyl sulfoxide denatured it into single strands of 1.5 micron and shorter fragments. it consists of 23.4% g, 23.4% c, 23.0% a, and 30.3% u and melts at a transition temperature of 81.7 degrees c in 75 mm nacl and 7.5 mm sodium citrate, ph 7.0, with 7 ... | 1985 | 2982874 |
| metronidazole radical anion generation in vivo in trichomonas vaginalis: oxygen quenching is enhanced in a drug-resistant strain. | the nitro radical-anion of metronidazole has been detected in vivo in the sexually transmitted human parasite, trichomonas vaginalis, under anaerobic conditions by electron spin resonance spectrometry. exposure of organisms to oxygen decreased the intensity of the radical signal in both metronidazole-sensitive atcc strain 30001 and in the metronidazole-resistant strain 85. the sensitive strain still gave radical signals at partial pressures of oxygen (greater than 6 kpa) sufficient to remove all ... | 1985 | 2985740 |
| isolation and differentiation of herpes simplex virus and trichomonas vaginalis in cell culture. | during the period january 1982 to january 1985, 2,234 specimens were cultured for isolation of herpes simplex virus (hsv). hsv was isolated from 23% of these, trichomonas vaginalis was isolated from 1.6%, and 75.3% were negative. in 0.2% of these, hsv and t. vaginalis were isolated from the same specimen. cytopathic effects produced by hsv were identified by their sensitivity to arabinosylthymine, whereas those produced by t. vaginalis were identified by their lack of sensitivity to arabinosylth ... | 1985 | 2993349 |
| epidemiology of cytomegaloviral infection in a heterogeneous population of pregnant women. | cervical cultures for cytomegalovirus (cmv) and samples of blood for antibody to cmv were obtained from 1,129 pregnant women: 57% of the women had antibody to cmv, and 14% of seropositive women shed virus. logistic regression analysis showed that seropositivity correlated with lower socioeconomic status, birth outside north america, multigravidity, older age, history of abnormal cervical cytology, infection with trichomonas vaginalis, a first pregnancy at less than or equal to 15 years of age, a ... | 1985 | 2993430 |
| monoclonal antibodies for the diagnosis of sexually transmitted diseases. | monoclonal antibodies are already being used for the diagnosis of human sexually transmitted diseases. these antibodies can be used to detect a wide range of microorganisms, including bacteria, parasites, and viruses. for both culture and direct tests, monoclonal antibodies showed patterns of specificity and reproducibility that exceeded those available with conventionally prepared antisera. the direct tests for these organisms required less than an hour to perform, representing a major advancem ... | 1985 | 2994946 |
| the generation of metronidazole radicals in hydrogenosomes isolated from trichomonas vaginalis. | the nitro radical-anion of the anti-trichomonal drug metronidazole has been detected by electron spin resonance spectrometry under anaerobic conditions in suspensions of intact hydrogenosomes isolated from the parasitic protozoon trichomonas vaginalis. metronidazole reduction was driven by pyruvate, but progressive damage to the radical generating system was observed. quenching of signals due to metronidazole radicals by chromium oxalate suggests that the radicals generated within the organelle ... | 1985 | 2999299 |
| inhibition of hydrogen production in drug-resistant and susceptible trichomonas vaginalis strains by a range of nitroimidazole derivatives. | | 1986 | 3000386 |
| energy-dispersive x-ray microanalysis of membrane-associated inclusions in hydrogenosomes isolated from trichomonas vaginalis. | energy-dispersive x-ray microprobe analysis of electron-dense inclusions in hydrogenosomes isolated from the aerotolerant anaerobic protozoal human parasite trichomonas vaginalis, bushby strain, indicated the presence of high levels of mg, p and ca. this suggested that divalent cation (e.g. ca2+ or mg2+) accumulation by hydrogenosomes may be important in the regulation of intracellular ion concentrations. | 1985 | 3005473 |
| respiration of trichomonas vaginalis. components detected by electron paramagnetic resonance spectroscopy. | | 1986 | 3007142 |
| [vulvovaginitis. etiopathogenesis and microbiological diagnosis]. | | 1986 | 3008032 |
| screening to reduce transmission of sexually transmitted diseases in semen used for artificial insemination. | the practice of artificial insemination by donor semen is increasing in the united states. many sexually transmitted organisms are found in semen, but screening procedures for the detection of these agents in donor semen have not been standardized. sexually transmitted organisms have been transmitted during artificial insemination by donor, and such transmission can cause local, disseminated, or fatal disease in the recipient woman and may harm the fetus or newborn. therefore, screening of both ... | 1986 | 3010110 |
| prevalence of genital pathogens among female prostitutes in new york city and in rotterdam. | the authors studied the prevalence of genital microorganisms among 300 female prostitutes in brothels in new york city and 60 female prostitutes attending a sexually transmitted diseases clinic in rotterdam, the netherlands. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma hominis, and ureaplasma urealyticum in the two cities were 9.3% and 8.3%, 25.3% and 16.6%, 57.3% and 74.9%, and 73% and 79%, respectively. trichomonas vaginalis was detected in 3.6% of new york pr ... | 1986 | 3012805 |
| [antiviral agents. 28. aliphatic substituted chlordihexylamino-1,3,5-triazine]. | the nucleophilic substitution of one chlorine atom in 2,4-dichloro-6-(dihexylamino)-1,3,5-triazine (1) by primary (2a-b) or secondary (2c) amines leads to the aliphatically substituted chloro-dihexylamino-1,3,5-triazines (3a-c) structures of type 3 may be characterized by the spectroscopic data. in the mass spectra, the complete degradation of the alkyl groupings is striking and manifested in a series of consecutive peaks with differences of 14 between each other. antiviral activity is exhibited ... | 1986 | 3013214 |
| glycerol, a metabolic end product of trichomonas vaginalis and tritrichomonas foetus. | glycerol was demonstrated as an end product of anaerobic glucose metabolism in trichomonas vaginalis and tritrichomonas foetus, produced in addition to acetate, h2, co2, and lactate or succinate. in t. vaginalis strain c-1, glycerol amounted to 16% of the fermentation products and was formed at an average rate of 38 nmol min-1 (mg protein)-1. corresponding figures for t. foetus strain kv1 were 7% and 4.8 nmol min-1 (mg protein)-1. the amounts of glycerol detected compensated almost exactly for t ... | 1986 | 3016535 |
| purine salvage enzymes in trichomonas vaginalis. | | 1986 | 3020926 |
| reduction of niridazole by metronidazole resistant and susceptible strains of trichomonas vaginalis. | the inhibitory effect of niridazole on hydrogen production by metronidazole-resistant (cdc-85) and susceptible (c1-nih) trichomonas vaginalis strains was investigated. the results show that niridazole is more effective than metronidazole in inhibiting hydrogen production by the resistant isolate. in cdc-85 aerobic inhibition requires a 4-fold increase in metronidazole concentration compared with that required anaerobically, but the corresponding factor for niridazole is only 1.5-fold. reduction ... | 1987 | 3029651 |
| phosphomonoesterases of leishmania mexicana mexicana and other flagellates. | amastigotes and log-phase promastigotes of leishmania mexicana mexicana contained distinct acid phosphatase, 3'-nucleotidase and 5'-nucleotidase activities, distinguishable by their response to ph and inhibitors. both tartrate-sensitive and tartrate-resistant acid phosphatase were present in the two forms, amastigotes possessed less tartrate-resistant acid phosphatase than promastigotes. a tartrate-sensitive acid phosphatase was secreted into the medium in large amounts during the growth in vitr ... | 1987 | 3037369 |
| nitroimidazole and oxygen derived radicals detected by electron spin resonance in hydrogenosomal and cytosolic fractions from trichomonas vaginalis. | hydrogenosome-enriched fractions from trichomonas vaginalis reduce a number of nitroimidazole derivatives to their respective electron spin resonance-detectable nitro-anion radicals. in the presence of of oxygen and 5,5-dimethyl-1-pyrroline-n-oxide (dmpo) a superoxide spin trapped adduct of dmpo was formed; the rate-determining step was the prior formation of the nitro-anion radical. oxygen-derived radicals were detected with cytosolic fractions from a metronidazole-resistant isolate (cdc-85) wh ... | 1987 | 3041214 |
| [pathogen spectrum of non-gonorrhea urethritis]. | by cultural investigations in 333 males with non-gonorrhoical urethritis (ngu) and 158 control probands it was demonstrated that chlamydia trachomatis is the main germ of this disease also in the gdr with a frequency of 40% of the cases. a chlamydia-negative ngu was caused in 15% by ureaplasma urealyticum in a number of greater than or equal to 10(4) germs/ml urine and in 5% by trichomonas vaginalis, respectively. candida albicans occurred significant more frequently in the patient group (21%) t ... | 1988 | 3043969 |
| new rapid latex agglutination test for diagnosing trichomonas vaginalis infection. | a newly developed latex agglutination test for trichomonas vaginalis infection was compared for sensitivity, specificity, efficiency, and positive and negative predictive values with microscopy, culture, and an enzyme linked immunosorbent assay (elisa) in the diagnosis of 395 women attending a genitourinary medicine clinic. t vaginalis infection was diagnosed in 42 (11%) women. the sensitivities of both the latex agglutination test and the elisa were 95% compared with 74% for microscopy and 76% ... | 1988 | 3045161 |
| analysis by immunofluorescence of alpha 1-antitrypsin levels on the surfaces of trichomonas vaginalis strains differing in virulence. | | 1988 | 3045793 |
| treatment failure in trichomonas vaginalis vaginitis. | | 1988 | 3049301 |
| alternating phenotypic expression of two classes of trichomonas vaginalis surface markers. | the antigenic heterogeneity of trichomonas vaginalis is due in part to the membrane disposition of immunogens (repertoire a) among some but not all isolates and among subpopulations of trichomonads of certain isolates. heterogeneous t. vaginalis isolates undergo phenotypic variation for the a repertoire of immunogens. the presence of immunogens on (a+ phenotype) or the absence of them from (a- phenotype) trichomonal surfaces clearly influences the virulence traits of the pathogenic human trichom ... | 1988 | 3055209 |
| [comparative study of the susceptibility of candida albicans and trichomonas vaginalis, on some products used as larvicides, trichomonacides and/or bactericides]. | | 1988 | 3066257 |
| localization of acetylated alpha-tubulin in tritrichomonas foetus and trichomonas vaginalis. | we used monoclonal antibodies specific for acetylated and nonacetylated alpha-tubulin to detect and to localize microtubules containing acetylated alpha-tubulin (stable microtubules) in the pathogenic protozoa tritrichomonas foetus and trichomonas vaginalis. sds-page analysis showed that tubulin is a major protein of both parasites, being enriched in cytoskeletal preparations of whole cells extracted with triton x-100. the monoclonal antibodies, which recognize all isoforms of alpha-tubulin (b-5 ... | 1988 | 3066505 |
| antiparasitic activity of nine pyrazole derivatives against trichomonas vaginalis, entamoeba invadens and plasmodium berghei. | nine nitropyrazole derivatives were prepared and tested against trichomonas vaginalis in vitro and in vivo, entamoeba invadens in vitro and plasmodium berghei in vivo. three of the compounds, 4-4-nitropyrazole, 1-methyl-4-nitropyrazole and 4,4'-dinitro-1,1'-methylenedipyrazole, have an activity similar to that of metronidazole (used as the reference compound) against t. vaginalis and e. invadens after 48 hours of incubation. all the compounds tested were inactive against p. berghei. | 1988 | 3074738 |
| [sexually transmitted infections in patients with condylomata acuminata]. | 42 patients (26 males, 16 females) with genital and perianal warts were submitted to serological testing for hbv markers, anti hsv antibodies and syphilis. specimens were also collected for microscopic and cultural examination for neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans and other pathogenic bacteria from urethra in men and from urethra, vagina and cervix in women. women had also cytologic examination from cervix with papanicolau method. we have found ... | 1986 | 3081835 |
| prevalence of nonspecific vaginitis and other cervicovaginal infections during the third trimester of pregnancy. | nonspecific vaginitis (nsv) is a clinical syndrome characterized by a decreased concentration of normal acidophilic döderlein flora in the vagina and increased concentration of nonacidophilic microorganisms, such as gardnerella vaginalis, anaerobic bacteria, and mycoplasmas. since several nsv-associated organisms are associated with complications of pregnancy, we studied the prevalence of nsv among 104 consecutive women during the third trimester of pregnancy. overall, 14 women (14%) had nsv by ... | 1986 | 3082020 |
| sexually transmissible infectious agents in sexually active and virginal asymptomatic adolescent girls. | sixty-eight sexually active and 52 virginal adolescent girls were evaluated for six sexually transmissible infectious agents: gardnerella vaginalis, ureaplasma urealyticum, mycoplasma hominis, chlamydia trachomatis, trichomonas vaginalis, and neisseria gonorrhoeae. there were significant differences between sexually active and virginal girls with respect to the prevalence of isolation of u urealyticum (75% v 33%, p less than .005), m hominis (27% v 10%, p less than .05), and c trachomatis (19% v ... | 1986 | 3083395 |
| microbiology of vaginal discharge in general practice. | three groups of women were examined by culture for gardnerella vaginalis and candida. group i consisted of 427 women, who complained spontaneously of vaginal discharge, group ii of 311 women who did not complain of vaginal discharge until questioned prior to gynaecological examination, and group iii of 100 women who denied vaginal discharge. groups i and ii also had cultures made for trichomonas vaginalis and neisseria gonorrhoica. in group i with spontaneous complaints the one-year prevalence r ... | 1986 | 3088694 |
| microflora in pregnancy. | | 1986 | 3090295 |
| polyamine biosynthesis in trichomonads. | trichomonas vaginalis, tritrichomonas foetus and trichomitus batrachorum grown in modified diamond's medium all had high concentrations of putrescine and lower concentrations of spermidine and spermine. ornithine decarboxylase (odc; ec 4.1.1.17) was detectable in all three species although at significantly different levels. trichomonas vaginalis had the highest activity (typically around 1.85 nmol min-1 (mg protein)-1), trichomitus batrachorum the lowest (0.11 nmol min-1 (mg protein)-1). the tri ... | 1986 | 3090433 |
| anaerobic pyruvate metabolism of tritrichomonas foetus and trichomonas vaginalis hydrogenosomes. | hydrogenosomes isolated from tritrichomonas foetus and trichomonas vaginalis fermented pyruvate to acetate, malate, h2, and co2 in an anaerobic process dependent on adp, pi, mg2+, and succinate. the extent to which pyruvate was carboxylated to malate by malate dehydrogenase (decarboxylating) rather than decarboxylated to acetate by pyruvate/ferredoxin oxidoreductase was dependent on pco2. the processes observed showed carbon and redox balances. the presence of an nadh/ferredoxin oxidoreductase a ... | 1986 | 3090435 |
| risk of transmission of sexually transmitted diseases by cryopreserved semen. | the increasing incidence of sexually transmitted diseases (std) involves the risk of the transmission of these diseases by the artificial insemination. therefore the cryotolerance of several microorganisms causing std and the efficiency of penicillin of the cryoprotective medium (cmp) against neisseria gonorrhoeae (n.g.) were investigated. the results have shown that penicillinase-producing n.g. (ppng), genital mycoplasmas and candida species survive the cryopreservation. trichomonas vaginalis c ... | 1986 | 3090904 |
| [heterogeneity of tubulins of parasitic protozoa]. | | 1986 | 3092715 |
| aminotransferase activities in trichomonas vaginalis. | a survey of aminotransferase activities present in a cell-free extract of the anaerobic protozoan, trichomonas vaginalis was performed. 2-oxoglutarate, oxaloacetate or phenylpyruvate acted as effective amino acceptors with tyrosine, phenylalanine, tryptophan, leucine, valine, isoleucine, aspartate, alanine, ornithine or lysine. arginine, serine, glutamine, glycine, beta-alanine and gamma-aminobutyrate were not active as amino donors. with pyruvate as acceptor, significant, yet low, activity was ... | 1986 | 3095639 |
| evaluation of new plastic envelope microbiology (pem) methods as adjuncts in the diagnosis of candida albicans and trichomonas vaginalis vaginitis. | | 1986 | 3095702 |
| s-adenosylmethionine and transmethylation reactions in trichomonads. | s-adenosylmethionine (sam) levels in trichomonads, a range of trypanosomatids and mouse liver were measured using hplc techniques. the concentrations were found to be similar in each with the exception of herpetomonas muscarum ingenoplastis, which contained approximately ten-fold more. living trichomonads were found to incorporate exogenous l-methionine into intracellular sam and its methyl carbon was also detected in lipids and nucleic acids, presumably through its involvement in transmethylati ... | 1987 | 3108875 |
| influence of microbial colonization on sperm-mucus interaction in vivo and in vitro. | two-hundred-and-thirty-three asymptomatic couples with a mean duration of infertility of 5 years were submitted to postcoital testing (pt) and to sperm penetration meter test (spmt) and simultaneous microbial screening. cervical swabs and semen specimens were collected for culture of mycoplasma hominis, ureaplasma urealyticum, chlamydia trachomatis, neisseria gonorrhoeae, other potentially pathogenic and commensal aerobic and anaerobic bacteria, herpes simplex virus, vaginal swabs for trichomona ... | 1987 | 3114313 |
| antimicrobial effects of some mono- and bishydrazones. | antibacterial, antifungal and antiprotozoal effects of nine mono- and bishydrazones of glycolaldehyde, glyoxal, methoxyacetaldehyde and glutaraldehyde were studied using eight model organisms. it was found that bishydrazones are much more efficient antimicrobial agents than monohydrazones in the case of all model microorganisms. | 1987 | 3117651 |
| sexually transmittable organisms in the urethra of males with and without urethritis. | in 589 males, 169 with and 420 without urethritis, urethral swabs were taken and assessed semiquantitatively for the sexually transmittable infectious agents neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, mycoplasma hominis, trichomonas vaginalis and candida species. the organisms were isolated in patients with and without urethritis as follows: n. gonorrhoeae with urethritis 19.5%, without 0.0%; c. trachomatis with urethritis 16.0%, without 2.9%; u. urealyticum (high cfu- ... | 1987 | 3118595 |
| antitrichomonal activity of alpha-difluoromethylornithine. | alpha-difluoromethylornithine, an inhibitor of polyamine biosynthesis, affected the pathogenicity of trichomonas vaginalis in two model systems. firstly, it blocked the cytotoxic effect of the parasite towards mammalian cells in culture: at a concentration of 1 mm it prevented the death of mouse myeloma cells in mixed culture with trichomonads. secondly, when administered orally to mice (multiple doses of 750 mg/kg body wt), the drug delayed the development of subcutaneous abscesses due to t. va ... | 1987 | 3119554 |
| antimicrobial properties of aromatic compounds of plant origin. | the antimicrobial action of 11 compounds involving guaiacyl- and syringyl-like structures (low-molecular-weight part of lignin), gallic acid and its derivatives, cinnamic acid and its derivatives, veratric acid, anisic acid and crotonic acid (a total of 25 compounds) against bacteria, yeast-like organisms and protozoa was examined. aromatic compounds modified in the c-side chain and aldehydes were effective preferentially against trichomonas vaginalis, whereas against bacteria and yeast-like org ... | 1987 | 3121479 |
| the prevalence of sexually transmitted disease agents in pregnant women in suva. | routine testing of 440 women (257 fijians, 183 indians) at the first antenatal attendance identified chlamydia trachomatis in 50% of fijians and 38% of indians; the seropositivity rates for syphilis were 14.2% and 1.7% respectively, and the isolation rates for n. gonorrhoeae were 3.1% in fijians and 1.1% in indians. | 1987 | 3124803 |
| prevalence of lower genital tract infections in pregnancy. | a descriptive profile of 106 pregnant women with various forms of cervicitis and vaginitis is provided. fifty women attending individual physicians' private offices are compared with 56 clinic patients: 34 attending a university prenatal teaching clinic and 22 attending a pregnancy termination unit. univariate analysis showed that single women were significantly more likely to be infected by mycoplasmas, yeasts, trichomonads, and gardnerella vaginalis than were married women. teenagers were more ... | 1988 | 3128883 |
| [the current status of trichomonas vaginalis infections of the male urogenital organ]. | | 1987 | 3130432 |
| some aspects of the diagnosis of specific vaginal infections in the rotterdam std clinic population. | we studied specific vaginal infections in 351 consecutive women who visited the rotterdam std clinic. prostitutes less often had vaginal or vulvar complaints than non-prostitutes (fisher test, p less than 0.01), but more often had abnormal discharge (fisher test, p less than 0.001). normal secretions were found in 52% of the women. clue cell-positive discharge (ccpd) was by far the most prevalent vaginal disease entity (32%). the wet mount showed pseudo-hyphae in 14 women (4%) and trichomonas va ... | 1988 | 3134262 |
| lower genital tract infections in women: comparison of clinical and epidemiologic findings with microbiology. | we screened 392 women attending the laboratory of microbiology of toulouse purpan hospital for symptoms of lower genital tract infection for six microorganisms. rates of isolation of neisseria gonorrhoeae, chlamydia trachomatis, candida albicans, mycoplasma hominis, and gardnerella vaginalis were 1%, 7.7%, 29.8%, 23.8%, and 37.5%, respectively. trichomonas vaginalis was detected by direct examination in specimens from 3.1% of patients. another goal of this study was to determine the relative pre ... | 1988 | 3135608 |
| [phagocytosis of streptococcus faecalis by trichomonas vaginalis. electron microscopy studies]. | | 1987 | 3136590 |
| the release of hydrolases from trichomonas vaginalis and tritrichomonas foetus. | trichomonas vaginalis and tritrichomonas foetus were found to release large amounts of beta-n-acetylglucosaminidase (ec 3.2.1.30), alpha-mannosidase (ec 3.2.1.24), beta-glucosidase (ec 3.2.1.21), acid phosphatase (ec 3.1.3.2) and proteinases during axenic growth in vitro. the enzymes were released continually throughout the growth phase, with the extracellular activity being of the same order as that within the cells. there was differential release of proteinases from trichomonas vaginalis. the ... | 1988 | 3140008 |
| gardnerella vaginalis is associated with other sexually transmittable microorganisms in the male urethra. | in a prospective study, urethral swabs were taken from 544 men presented to an std clinic, 118 with and 426 without urethritis, and examined by microscope and/or culture for g. vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, ureaplasma urealyticum, mycoplasma hominis, candida species and trichomonas vaginalis. g. vaginalis was isolated from 4.2% of the males with urethritis and from 6.3% of those without urethritis. using loglinear analysis, the following associations were significant ( ... | 1988 | 3140532 |
| comparison of four methods to detect trichomonas vaginalis. | four methods for the detection of trichomonas vaginalis in vaginal secretions from 88 symptomatic patients were compared: wet-mount examination, kupferberg liquid medium, hirsch charcoal agar, and the papanicolaou smear. hirsch diphasic slants and kupferberg medium did not significantly differ in sensitivity from direct examination of wet mounts. the papanicolaou smear identification of trichomonads was found to be the least sensitive method evaluated. | 1988 | 3141470 |
| effect of dl-alpha-difluoromethylornithine on polyamine synthesis and interconversion in trichomonas vaginalis grown in a semi-defined medium. | growth of trichomonas vaginalis in a semi-defined medium was inhibited by 5 mm dl-alpha-difluoromethylornithine (dfmo). using high pressure liquid chromatography (hplc) analysis, putrescine and cadaverine levels were found to be 90 and 100% reduced, respectively after 120 h exposure, whilst spermidine and spermine levels were unchanged. putrescine (40 microm) and cadaverine (6 microm) were detected in the spent media from control cultures. neither of these diamines was detected in spent media fr ... | 1988 | 3141809 |
| microbiological profile of the cervix in 1,000 sexually active women. | one thousand consecutive women who attended the richmond family planning association clinic and who were to undergo a vaginal examination were asked to participate in a large prospective microbiological study. participants were questioned about their sexual activity during the previous 12 months and any apparent signs of sexually transmitted disease. on examination the cervix was inspected for evidence of inflammation, ectopy or discharge and cervical swabs were taken microbiological assessment. ... | 1988 | 3148302 |
| [the microbiological characteristics of bacterial vaginosis]. | | 1987 | 3154365 |
| basic properties of tritrichomonas mobilensis hemagglutinin. | tritrichomonas mobilensis is a recently described enteric protozoon of squirrel monkeys. an earlier report identified one of the metabolic products of this organism as a lectinlike hemagglutinin. its further properties were determined in this study. culture supernatants of t. mobilensis fp4190 were concentrated by ultrafiltration through a membrane with 100,000-molecular-weight cutoff. high titers of agglutinin against human erythrocytes were obtained. incubation at 70 degrees c for 15 min resul ... | 1988 | 3170709 |
| the interaction of trichomonas vaginalis and tritrichomonas foetus with epithelial cells in vitro. | the madin-darby canine kidney epithelial cell line (mdck) was used as a model for trichomonad-host cell interaction. two laboratory strains of the human parasite trichomonas vaginalis and the cattle's parasite tritrichomonas foetus or their supernatants from axenic cultures were allowed to interact with confluent epithelial cultures. the interaction process studied by transmission and scanning electron microscopy revealed that both parasites adhere to monolayers through flagella, cell body and p ... | 1988 | 3180257 |
| effects of antimicrobial therapy on sperm-mucus interaction. | sperm-mucus interaction under in-vitro or in-vivo conditions might be affected by microorganisms colonizing the reproductive tract. in order to study the influence of antimicrobial therapy, an extensive microbial screening was performed including chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum, neisseria gonorrhoeae, a broad spectrum of potentially pathogenic aerobic and anaerobic bacteria, trichomonas vaginalis, herpes simplex virus and yeasts. one-hundred-and-six couples with ... | 1988 | 3182976 |
| prostitution in sheffield: differences between prostitutes. | this study to assess differences between street walking prostitutes and sauna girls who attended this clinic in 1986 and 1987 showed that fewer street walkers used barrier methods for disease prevention with clients or accepted tests for antibody to human immunodeficiency virus (hiv) when offered and more street walkers practised their profession in both sheffield and london. they therefore represent a potential pathway for the heterosexual spread of hiv to the region. trichomonas vaginalis was ... | 1988 | 3224974 |
| giardia-bile salt interactions in vitro and in vivo. | giardia lamblia predominantly colonizes the proximal small intestine where bile is plentiful. we have investigated interactions between bile and this parasite by (i) examining the specificity of the stimulatory effect of bile on parasite growth in vitro, (ii) studying the possible association between giardiasis and bile salt deconjugation in vivo, and (iii) quantifying bile salt uptake by giardia and relating this to uptake by other microorganisms. our findings indicate that the growth promoting ... | 1988 | 3232181 |
| giardia duodenalis: enhanced growth in cell culture. | growth of giardia duodenalis in broth and in animals has been studied in considerable detail. in contrast, the kinetics of growth in cell culture have been little evaluated. in this study, in vitro growth of g. duodenalis was evaluated in cell culture, primarily using mouse mccoy cells in vials. the media used were giardia broth (tyi-g), trichomonas vaginalis broth (tyi-t), and standard cell culture media (cmga) alone and in combination (2 parts by volume cmga to one part of tyi broth). addition ... | 1988 | 3232182 |
| multiple populations of double-stranded rna in two virus-harbouring strains of trichomonas vaginalis. | the existence of six dsrna segments of trichomonas vaginalis virus was confirmed and the molar mass and relative abundance of these segments were determined by agarose gel electrophoresis with reovirus dsrna serving as a standard. the m's were 3.5, 3.4, 3.2, 2.5, 1.4 and 0.34 mg/mol for the two strains studied, the relative abundances, however, were 1.0, 1.4, 3.0, 0.3, 2.7, 4.2 and 1.0, 0.6, 1.7, 0.5, 3.4 1.0 for these strains, respectively. cell homogenate fractionation showed that all dsrna se ... | 1988 | 3240907 |
| [cyto-microbiologic study of cervico-vaginal specimens, associated with a serologic study to detect sexually transmissible diseases in madagascan prostitutes]. | a microbiological survey of cervical and vaginal smears associated with a serological survey of sexual diseases was carried out on 298 prostitutes at the four principal malagasy sea ports. this survey leads us to these conclusions: the great frequency of specific inflammations (72.1%) with trichomonas vaginalis in the first place (39.2%). the serological tests proved a high rate of positive treponemical serologies (25.2%), whose the half of the cases are evolutive forms. nevertheless prevalence ... | 1988 | 3242427 |
| identification and properties of trichomonas vaginalis proteins involved in cytadherence. | trichomonas vaginalis nyh286 surface proteins which are candidates for mediating parasite cytadherence (adhesins) were identified. at least four trichomonad protein ligands ranging in relative molecular mass from 65 to less than or equal to 21 kilodaltons were found to selectively bind to chemically stabilized hela cells. the proteins were present on the surfaces of 10 different isolates of t. vaginalis examined; however, the nonpathogenic trichomonad t. tenax did not possess similar hela cell-b ... | 1988 | 3257206 |
| the diagnosis of vaginal trichomoniasis. | | 1988 | 3257533 |
| the purification and properties of two soluble reduced nicotinamide: acceptor oxidoreductases from trichomonas vaginalis. | the occurrence of soluble reduced nicotinamide nucleotide:acceptor oxidoreductases has been reported in a number of strains of the oxygen-tolerant anaerobe trichomonas vaginalis and other trichomonad species. the quantitatively more important enzyme in most strains of t. vaginalis is an nadh oxidase which produces water from the reduction of oxygen. this enzyme has been purified by a combination of gel filtration, chromatofocusing, cibacron blue chromatography and high pressure gel permeation ch ... | 1988 | 3257811 |
| fewer trichomonas vaginalis organisms in vaginas of infected women during menstruation. | the aims of the present study were to count trichomonas vaginalis organisms recovered from the vaginas of patients with trichomoniasis, and to obtain data concerning changes in sizes of trichomonal populations during the menstrual cycle. in about 80% of symptomatic as well as symptomless patients, more than 1 x 10(5) parasites per ml could be obtained from vaginal washes. during menstruation, however, the number of trichomonads decreased appreciably, with subsequent increases within three to six ... | 1988 | 3257936 |
| in vitro susceptibility of trichomonas vaginalis to 50 antimicrobial agents. | we determined the susceptibilities of five strains of trichomonas vaginalis, one of which was metronidazole resistant, to 50 antimicrobial agents. for the metronidazole-susceptible strains, the most active agents were metronidazole, tinidazole, mebendazole, furazolidone, and anisomycin. against the resistant strain mebendazole, furazolidone, and anisomycin were the most active. antifungal agents, beta-lactams, macrolides, aminoglycosides, and folic acid antagonists were ineffective against all s ... | 1988 | 3258142 |
| use of a crude extract or a purified antigen of trichomonas vaginalis for the detection of secretory antibodies by elisa. | a crude aqueous extract (t2) or a gel-filtration purified antigen of t. vaginalis was utilized to detect secretory antibodies in cervico-vaginal secretions by elisa. the test was developed under optimum conditions using a rabbit anti-trichomonas serum. conditions established for monitoring antibodies to trichomonas in immunized rabbits were equally effective for human secretions. the crude extract was capable of assessing secretory antibodies in 45% of women with acute trichomoniasis and 43% of ... | 1988 | 3258398 |
| mouse spleen cell responses to trichomonal antigens in experimental trichomonas vaginalis infection. | subcutaneous inoculation of live t. vaginalis into mice caused splenomegaly, particularly when using strains of parasites with low pathogenicity. the proliferative responses of spleen cells from uninfected mice, as measured by [3h] tdr uptake, showed that trichomonal antigens, whether from strains with high or low pathogenicity, have no mitogenic activity. spleen cells from mice infected with trichomonads of low pathogenicity showed a proliferative response to trichomonal antigens that was maxim ... | 1988 | 3258637 |
| in vitro susceptibility of trichomonas vaginalis to metronidazole and treatment outcome in vaginal trichomoniasis. | we have identified trichomonas vaginalis strains resistant in vitro to metronidazole, especially under aerobic conditions. since little is known about the relationship of treatment outcome to metronidazole susceptibility of t. vaginalis, we studied the aerobic and anaerobic susceptibility to metronidazole of 310 clinical isolates of t. vaginalis. of 199 patients with known outcomes after metronidazole treatment for vaginal trichomoniasis, the geometric mean minimal lethal concentration (mlc) und ... | 1988 | 3258675 |
| determination of cytopathogenic effects of trichomonas vaginalis on cultured cells by assaying 51cr release. | by use of an assay for 51cr release, it was attempted to determine the cytopathogenic effects of trichomonas vaginalis on mammalian cultured cells. 51cr was released from na2(51)cro4-labeled hela, l-929, fl, vero and llc-mk 2 cells. hela cells were most sensitive to the damage, followed by l-929, fl, vero and llc-mk 2 cells. the effect depended upon the number of parasite and the incubation period. the maximum 51cr release from the labeled cells was observed at 37 degrees c and ph 6.0. these res ... | 1988 | 3259767 |
| flagella-bearing bare trichomonas vaginalis nuclei in papanicolaou smears. | | 1988 | 3259922 |
| [anthelmintic agents. 5. long-chain and hydroheterocyclic-substituted chloro-diallylamino-1,3,5-triazines]. | | 1988 | 3260475 |
| differential susceptibility of fresh trichomonas vaginalis isolates to complement in menstrual blood and cervical mucus. | the ability of complement in human menstrual blood and cervical mucus to kill trichomonas vaginalis was compared with that of complement in serum, and 95 fresh trichomonal isolates obtained from vaginal wash material were evaluated for susceptibility to complement immediately after isolation. only serum and menstrual blood with haemolytic activity produced total cytolysis of t vaginalis. the cytolytic abilities of these fluids were totally inactivated by treatment with heat or edetic acid (edta) ... | 1988 | 3261708 |
| specific parasitism of purified vaginal epithelial cells by trichomonas vaginalis. | human vaginal epithelial cells (vecs) from vaginal swabs obtained from normal women or from patients with trichomoniasis were purified, and vec parasitism by trichomonas vaginalis was examined. trichomonads bound equally well to live or dead vecs, and up to 20% of vecs were parasitized. trichomonal cytadherence of human vecs was time, temperature, and ph dependent. saturation binding levels of live trichomonads to vecs gave approximately 2 organisms adherent to parasitized vec. no differences in ... | 1988 | 3262088 |
| fewer trichomonas vaginalis organisms in vaginas of infected women during menstruation. | | 1988 | 3262565 |
| [observation on trichomonas vaginalis by scanning electron microscopy]. | | 1988 | 3263220 |
| monoclonal-antibody-based enzyme-linked immunosorbent assay for trichomonas vaginalis. | trichomonas vaginalis is estimated to infect 4 million women per year in the united states. the diagnosis of trichomoniasis is predominantly achieved by direct microscopic examination of vaginal exudates. this subjective diagnostic procedure is reported to be 75% sensitive under ideal circumstances. we have developed an enzyme-linked immunosorbent assay (elisa) for the detection of t. vaginalis directly from vaginal exudates. the elisa employs a monoclonal antibody specific for a 65-kilodalton s ... | 1988 | 3263386 |
| demonstration of concanavalin a- and wheat germ agglutinin-binding sites on the trichomonas vaginalis surface coat using lectin-gold particle conjugates. | | 1988 | 3263459 |
| [morphology and biological activity of trichomonas vaginalis grown on various nutrient media]. | | 1988 | 3264099 |
| [ultrastructural analysis of the interrelation of the causative agents of urogenital infections with the cells of the macro-organism in syphilis patients]. | | 1988 | 3264100 |
| effects of antimicrobial agents on growth and chemotaxis of trichomonas vaginalis. | the motility of viable trichomonas vaginalis organisms is readily demonstrable in a clinical wet mount or cultured specimens. we attempted to determine whether migration is a dynamic process such that the organisms move to avoid exposure to toxic antimicrobial agents. with the use of axenic cultures of t. vaginalis that were radiolabeled and assayed for chemotaxis in plastic multiwelled plates with a membrane filter inserted to trap organisms, the response of clinical isolates to various antimic ... | 1988 | 3264130 |
| [verification of the value of the elisa test in the diagnosis of urogenital trichomoniasis]. | | 1988 | 3264747 |
| [use of the elisa method in the determination of antibodies to trichomonas vaginalis]. | | 1988 | 3264749 |
| [complement-binding antibodies in women simultaneously infected with various species of trichomonas]. | | 1988 | 3265240 |
| [intradermal test with trichomonas vaginalis allergen in patients with urogenital trichomonas infection]. | | 1988 | 3265243 |
| the effect of hormones on trichomonas vaginalis. | the hormonal milieu can alter susceptibility to infection. the effect of hormones on trichomonas vaginalis was studied utilizing axenically cultured clinical isolates. oestrogens, in physiological concentrations, decreased the growth of the organisms and their attachment to mammalian cells in vitro, and acted as a chemorepellent. the specificity of these effects was verified by their being blocked with anti-oestrogens, by the dose- and time-dependency of the responses, and by the lack of effect ... | 1988 | 3265429 |
| [a dried nutrient medium made from the fermented hydrolysates of a microorganism biomass for isolating trichomonas vaginalis]. | | 1988 | 3265559 |
| human amnion membrane as a model for studying the host-parasite relationship in trichomoniasis. | | 1988 | 3265702 |
| isoenzyme patterns of isolates of trichomonas vaginalis from vancouver. | isoenzyme patterns of 63 isolates of trichomonas vaginalis obtained in vancouver were evaluated by use of thin-layer starch-gel electrophoresis. we attempted to use eight enzymes, but only four gave reproducible and interpretable results. there were four patterns with malic enzyme, two with malate dehydrogenase, one with hexokinase, and four with lactate dehydrogenase. the isoenzyme patterns of the 63 isolates were classified into 15 groups, but 49 (78%) fell into five groups and 14 (22%) fell i ... | 1988 | 3265809 |
| [sensitivity of trichomonas vaginalis to desiccation]. | | 1988 | 3266051 |
| trichomonas vaginalis: preliminary characterization of a sperm motility inhibiting factor. | this study determined the effects of trichomonas vaginalis trophozoites, subcellular fractions, and medium from axenic t. vaginalis cultures on human sperm motility and viability. spent medium (ph 7.0) caused complete cessation of sperm motility after 15 minutes incubation. trophozoite soluble fraction or formalin-killed trophozoites caused a 50 percent reduction in sperm motility, compared to 25 percent reduction caused by the trophozoite particulate fraction or the sterile medium and three per ... | 1988 | 3266454 |
| effect of metronidazole on the cell surface charge of trichomonas vaginalis and tritrichomonas foetus. | 1. the effect of metronidazole, a drug used in the treatment of trichomoniasis, on the surface charge of both trichomonas vaginalis and tritichomonas foetus was analyzed by cell electrophoresis. 2. incubation of the parasites under anaerobic conditions for 2 or 120 min in the presence of 2.0 micrograms/ml metronidazole, a concentration which inhibits cell growth by about 50%, led to a marked decrease in the net surface charge of the parasites. 3. the metronidazole analogue, 1-hydroxyethyl-2-meth ... | 1988 | 3266844 |
| [trichomonas vaginalis, epithelial cells, spermatozoa, and immunologic reactions. how and why an infection persists unchanged despite knowing an adequate drug therapy]. | | 1988 | 3267183 |