| analysis of mineral and heavy metal content of some commercial fruit juices by inductively coupled plasma mass spectrometry. | the presence of potentially toxic elements and compounds in foodstuffs is of intense public interest and thus requires rapid and accurate methods to determine the levels of these contaminants. inductively coupled plasma mass spectrometry is a powerful tool for the determination of metals and nonmetals in fruit juices. in this study, 21 commercial fruit juices (apple, peach, apricot, orange, kiwi, pear, pineapple, and multifruit) present on romanian market were investigated from the heavy metals ... | 2013 | 24453811 |
| inhibition of nonenzymatic protein glycation by pomegranate and other fruit juices. | the nonenzymatic glycation of proteins and the formation of advanced glycation endproducts in diabetes leads to the crosslinking of proteins and disease complications. our study sought to demonstrate the effect of commonly consumed juices (pomegranate, cranberry, black cherry, pineapple, apple, and concord grape) on the fructose-mediated glycation of albumin. albumin glycation decreased by 98% in the presence of 10 μl of pomegranate juice/ml; other juices inhibited glycation by only 20%. pomegra ... | 2014 | 24433074 |
| low temperature alters plasma membrane lipid composition and atpase activity of pineapple fruit during blackheart development. | plasma membrane (pm) plays central role in triggering primary responses to chilling injury and sustaining cellular homeostasis. characterising response of membrane lipids to low temperature can provide important information for identifying early causal factors contributing to chilling injury. to this end, pm lipid composition and atpase activity were assessed in pineapple fruit (ananas comosus) in relation to the effect of low temperature on the development of blackheart, a form of chilling inju ... | 2014 | 24390546 |
| bromelain inhibits allergic sensitization and murine asthma via modulation of dendritic cells. | the incidence of atopic conditions has increased in industrialized countries. persisting symptoms and concern for drug side-effects lead patients toward adjunctive treatments such as phytotherapy. previously, we have shown that bromelain (sbr), a mixture of cysteine proteases from pineapple, ananas comosus, inhibits ovalbumin (ova)-induced murine model of allergic airway disease (aad). however, sbr's effect on development of aad when treatment is administered throughout ova-alum sensitization wa ... | 2013 | 24381635 |
| enhanced production of xylanase by solid state fermentation using trichoderma koeningi isolate: effect of pretreated agro-residues. | the main objective of this study was to isolate the fungal strain for enhanced production of xylanase using different agro-residues and fruit peels by solid state fermentation and its potentiality was tested on the pretreated corn cob. fermentation was carried out with trichoderma koeningi isolate using untreated and pretreated corn cob supplemented with pineapple peel powder showed higher production of xylanase 2,869.8 ± 0.4 (iu/g) and extracellular protein 7.6 ± 0.2 (mg/g) of corn cob, in the ... | 2014 | 28324314 |
| alteration of porcine serum albumin levels in pork meat by marination in kiwi or pineapple juice and subsequent pan broiling. | this study was conducted to evaluate the changes in porcine serum albumin (psa), a major allergen, which occur when raw pork ham is marinated with kiwi or pineapple juice, and/or when the ham is pan broiled at 300℃ for 4 min after marination. in this study, raw pork ham was soaked for 4 h or 8 h in marinades containing commercial marinating sauce only, commercial marinating sauce and 7% kiwi juice, or commercial marinating sauce and 7% pineapple juice. when the meat was marinated and then pan-br ... | 2014 | 26761177 |
| influence of harvest maturity and fruit logistics on pineapple (ananas comosus [l.] merr.) volatiles assessed by headspace solid phase microextraction and gas chromatography-mass spectrometry (hs-spme-gc/ms). | profiling of volatiles from pineapple fruits was performed at four ripening stages using headspace solid-phase microextraction and gas chromatography-mass spectrometry (hs-spme-gc/ms). in total, 142 volatiles were detected, of which 132 were identified. multivariate data analysis was carried out to assess the effect of post-harvest storage on volatiles composition of green-ripe sea-freighted pineapple in comparison to air-freighted fruits harvested at full maturity. the latter fruits were charac ... | 2014 | 24360466 |
| validation of a gc-ms method for the estimation of dithiocarbamate fungicide residues and safety evaluation of mancozeb in fruits and vegetables. | a sensitive and rugged residue analysis method was validated for the estimation of dithiocarbamate fungicides in a variety of fruit and vegetable matrices. the sample preparation method involved reaction of dithiocarbamates with tin(ii) chloride in aqueous hcl. the cs2 produced was absorbed into an isooctane layer and estimated by gc-ms selected ion monitoring. limit of quantification (loq) was ⩽40μgkg(-1) for grape, green chilli, tomato, potato, brinjal, pineapple and chayote and the recoveries ... | 2014 | 24360436 |
| prospective double-blind clinical trial evaluating the effectiveness of bromelain in the third molar extraction postoperative period. | to evaluate the anti-inflammatory and analgesic effect of bromelain (pineapple extract) administered orally in the postoperative after extraction of impacted lower molars. | 2014 | 24316697 |
| surface plasmon resonance optical sensor and antibacterial activities of biosynthesized silver nanoparticles. | silver nanoparticles were prepared using aqueous fruit extract of ananas comosus as reducing agent. these silver nanoparticles showed surface plasmon peak at 439 nm. they were monodispersed and spherical in shape with an average particle size of 10 nm. the crystallinity of these nanoparticles was evident from clear lattice fringes in the hrtem images and bright circular spots in the saed pattern. the antibacterial activities of prepared nanoparticles were found to be size-dependent, the smaller ... | 2014 | 24291437 |
| a superoxide dismutase purified from the roots from stemona tuberosa. | proteins from the fresh roots of stemona tuberosa (stemonaceae) were extracted into 20 mm phosphate buffer, ph 7.2/0.1 m nacl, precipitated with 90% saturation ammonium sulfate, and enriched by diethylaminoethanol (deae) cellulose. the protein eluted as a single main peak from the unbound fractions (st-1), and appeared as a single band with superoxide dismutase (sod) activity after native polyacrylamide gel electrophoresis (page) resolution and zymogram development. st-1 was classified as sod du ... | 2014 | 24279794 |
| sequential multi-layered hemostatic sutures for stepwise removal of arteriovenous malformations: the pineapple technique. | | 2014 | 24268693 |
| assessment of fruit juice authenticity using uplc-qtof ms: a metabolomics approach. | in recent years, with the growing complexity of global food supply chains and trade, food fraud, including adulteration of high value foods with cheaper substitutes, has become an increasingly important issue. a metabolomics approach can be applied to discover biomarkers that can be used to trace food adulteration. a study was undertaken to discover novel, potential biomarkers for the rapid detection of the adulteration of fruit juices with cheaper alternatives. pineapple, orange, grapefruit, ap ... | 2014 | 24262519 |
| incomplete stomach emptying as a complication of intragastric balloon treatment and a solution suggestion: pineapple juice drinking. | during removal of intragastric balloon, there is a great deal of gastric undigested food even after an eight-hour starvation. bromelain, a proteolytic enzyme existing in the pineapple juice seems to be a good choice for the undigested food remnants in the stomach. we aimed to investigate the effect of drinking pineapple juice on dissolving food remnants in patients undergoing endoscopic procedure for removal of intragastric balloon. | 2013 | 24254264 |
| pharmacological potential and conservation prospect of the genus eucomis (hyacinthaceae) endemic to southern africa. | the genus eucomis (hyacinthaceae) consists of 10 species that are extensively used in african traditional medicine. this review is an appraisal of current information on the distribution and morphology, traditional uses, pharmacology, toxicology and approaches devised to enhance the conservation of the genus. | 2014 | 24247080 |
| errors and myths in feeding and nutrition: impact on the problems of obesity. | the increase in obesity prevalence cannot be explained by a sudden and generalized change in human genome. it is certainly due to the modification of lifestyle habits and especially of the diet, as well as a lack of physical activity and sedentary living. changes in the feeding pattern and the subsequent unbalance in the caloric profile of the diet may have had great importance in the occurrence of obesity. the social pressure in relation to the body image, the desire to have a slim body, and th ... | 2013 | 24010747 |
| the chemopreventive action of bromelain, from pineapple stem (ananas comosus l.), on colon carcinogenesis is related to antiproliferative and proapoptotic effects. | colorectal cancer is an important health problem across the world. here, we investigated the possible antiproliferative/proapoptotic effects of bromelain (from the pineapple stem ananas comosus l., family bromeliaceae) in a human colorectal carcinoma cell line and its potential chemopreventive effect in a murine model of colon cancer. | 2014 | 24123777 |
| enzymatic, antimicrobial and toxicity studies of the aqueous extract of ananas comosus (pineapple) crown leaf. | various parts of the plant pineapple (ananas comosus) are used in traditional medicine worldwide for treatment of a number of diseases and disorders. in folk medicine, pineapple leaf extract was used as an antimicrobial, vermicide, purgative, emmenagoogue, abortifacient, anti-oedema and anti-inflammatory agent. compared to the fruit and stem extracts of pineapple, information about its leaf extract is limited. the potential of pineapple crown leaf extract as an ethno-medicine has been evaluated ... | 2013 | 24076462 |
| separation of a binary mixture of pesticides in fruits using a flow-through optosensor. | a flow-through optosensor is here proposed for the determination of mixtures of two widely used pesticides, carbendazim and o-phenylphenol, in fruits. the pesticides are separated on-line using an additional amount of solid support, c18 silica gel, in the flow-through cell. the resolution is performed due to the different retention/desorption kinetics of the analytes when interacting with the c18 microbeads. therefore, both separation and determination are integrated in the same cell, considerab ... | 2013 | 24054619 |
| quantification of bioactive compounds in pulps and by-products of tropical fruits from brazil. | this study aimed to quantify the levels of resveratrol, coumarin, and other bioactives in pulps and by-products of twelve tropical fruits from brazil obtained during pulp production process. pineapple, acerola, monbin, cashew apple, guava, soursop, papaya, mango, passion fruit, surinam cherry, sapodilla, and tamarind pulps were evaluated as well as their by-products (peel, pulp's leftovers, and seed). total phenolic, anthocyanins, yellow flavonoids, β-carotene and lycopene levels were also deter ... | 2014 | 24054258 |
| identification of transcription factors potentially involved in the juvenile to adult phase transition in citrus. | the juvenile to adult transition (jat) in higher plants is required for them to reach reproductive competence. however, it is a poorly understood process in woody plants, where only a few genes have been definitely identified as being involved in this transition. this work aims at increasing our understanding of the mechanisms regulating the jat in citrus. | 2013 | 24052558 |
| development of pineapple microsatellite markers and germplasm genetic diversity analysis. | two methods were used to develop pineapple microsatellite markers. genomic library-based ssr development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 simple sequence repeat (ssr) loci were dinucleotide repeats (39 ac/gt repeats and 52 ga/tc repeats, accounting for 42.9% and 57.1%, resp.), and the other three were mononucleotide repeats. thirty-six pairs of ssr primers were designed; 24 of them generated clear ... | 2013 | 24024187 |
| polymer-based alternative method to extract bromelain from pineapple peel waste. | bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (ananas comosus merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. the use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. we aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-ph ... | 2016 | 24011234 |
| activation of words with phonological overlap. | multiple lexical representations overlapping with the input (cohort neighbors) are temporarily activated in the listener's mental lexicon when speech unfolds in time. activation for cohort neighbors appears to rapidly decline as soon as there is mismatch with the input. however, it is a matter of debate whether or not they are completely excluded from further processing. we recorded behavioral data and event-related brain potentials (erps) in auditory-visual word onset priming during a lexical d ... | 2013 | 24009593 |
| the physiology of ex vitro pineapple (ananas comosus l. merr. var md-2) as cam or c3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. | proteomic and transcriptomic profiles of key enzymes were monitored in pineapple plants propagated under c3 and cam-inducing metabolisms to obtain insight into the cam-facultative metabolism and the relationship of cam plants with oxidative stress. | 2013 | 23959598 |
| adding pineapple juice to a polyethylene glycol-based bowel cleansing regime improved the quality of colon cleaning. | an unsuccessful colonoscopy procedure is often related to inadequate bowel cleansing. it is difficult for patients to finish the whole 4 liters of polyethylene glycol-electrolyte lavage (peg-el) because of its salty taste and the large quantity. pineapple juice has been shown to be an effective agent in the dissolution of undigested food in the stomach. this study assessed the effectiveness of both 2 and 4 liters of peg-el in precolonoscopic bowel cleansing and the quality of colonoscopic cleani ... | 2013 | 23949576 |
| fruit juice drinks prevent endogenous antioxidant response to high-fat meal ingestion. | high-fat meals (hfm) induce metabolic stress, leading to the activation of protective mechanisms, including inflammation and endogenous antioxidant defences. in the present study, we investigated the effects of antioxidant-rich fruit juice drinks on the endogenous antioxidant response induced by hfm. in a double-blind, cross-over design (10 d washout), fourteen overweight volunteers were randomly assigned to one of the following interventions: hfm+500 ml placebo beverage (hfm-pb, free from fruit ... | 2014 | 23930843 |
| an in vitro study reveals nutraceutical properties of ananas comosus (l.) merr. var. mauritius fruit residue beneficial to diabetes. | rapid urbanisation and nutritional transition is fuelling the increased global incidence of type 2 diabetes. pineapple fruit residue was explored for its nutraceutical properties as an alternative or adjunct to currently available treatment regime. ethyl acetate and methanolic extracts of pineapple fruit residue were evaluated for anti-diabetic activity in cell free and cell based systems. specifically, we assessed: (1) antioxidant potential, (2) anti-glycation potential, (3) carbohydrate digest ... | 2014 | 23929507 |
| serving a variety of vegetables and fruit as a snack increased intake in preschool children. | although serving a greater variety of food increases intake, this effect has not been well studied as a strategy to encourage consumption of vegetables and fruit in preschool children. | 2013 | 23902783 |
| pectin methylesterase of datura species, purification, and characterization from datura stramonium and its application. | pectin methylesterases (pme; ec 3.1.1.11) involved in de-esterification of pectin and have applicability in food, textiles, wines, pulp, and paper industries. in the present study, we compared pme activity of different parts of 3 datura species and found that fruit coat showed maximum pme activity followed by leaf and seed. pme from leaves of d. stramonium (dspme) was purified and characterized. dspme showed optimum activity at 60 °c and ph 9 in the presence of 0.3 m nacl. dspme was stable at 70 ... | 2013 | 23887498 |
| in vitro bacterial fermentation of tropical fruit fibres. | tropical fruits such as mango, papaya, pineapple and banana are rich sources of dietary fibre. however, few studies have examined the potential physiological effects of fibre from these tropical fruits. the aim of this study was to characterise the fermentability of dietary fibre found in banana, papaya, pineapple and mango as an estimate of the physiological effects of consuming these fruits. freeze-dried fruit was subjected to in vitro digestion to remove digestible carbohydrates. digestion re ... | 2013 | 23887033 |
| a novel aspartic acid protease gene from pineapple fruit (ananas comosus): cloning, characterization and relation to postharvest chilling stress resistance. | a full-length cdna encoding a putative aspartic acid protease (acap1) was isolated for the first time from the flesh of pineapple (ananas comosus) fruit. the deduced sequence of acap1 showed all the common features of a typical plant aspartic protease phytepsin precursor. analysis of acap1 gene expression under postharvest chilling treatment in two pineapple varieties differing in their resistance to blackheart development revealed opposite trends. the resistant variety showed an up-regulation o ... | 2013 | 23838125 |
| deconstructing a fruit serving: comparing the antioxidant density of select whole fruit and 100% fruit juices. | research suggests phytonutrients, specifically phenolic compounds, within fruit may be responsible for the putatively positive antioxidant benefits derived from fruit. given the prominence of fruit juice in the american diet, the purpose of this research was to assess the antioxidant density of fresh fruit and 100% fruit juice for five commonly consumed fruits and juices and to compare the adequacy of 100% juice as a dietary equivalent to whole fruit in providing beneficial antioxidants. antioxi ... | 2013 | 23810279 |
| using choice experiments to understand household tradeoffs regarding pineapple production and environmental management in costa rica. | choices among environmental management alternatives involve tradeoffs where, for example, the benefits of environmental protection may be offset by economic costs or welfare losses to individual agents. understanding individual or household-level preferences regarding these tradeoffs is not always straightforward, and it often requires an analysis of choices under alternative scenarios. a household survey was used to gather data for a choice experiment, where respondents were asked to choose amo ... | 2013 | 23807434 |
| the expression patterns of bromelain and accys1 correlate with blackheart resistance in pineapple fruits submitted to postharvest chilling stress. | blackheart is a physiological disorder induced by postharvest chilling storage during pineapple fruit export shipping. the aim of this study was to check the involvement of bromelain, the cysteine protease protein family abundantly present in pineapple fruits, and accys1, an endogenous inhibitor of bromelain, in the development of blackheart. for this we checked the response to postharvest chilling treatment of two pineapple varieties (md2 and smooth cayenne) differing in their resistance to bla ... | 2013 | 23777839 |
| bromelain down-regulates myofibroblast differentiation in an in vitro wound healing assay. | bromelain, a pineapple-derived enzyme mixture, is a widely used drug to improve tissue regeneration. clinical and experimental data indicate a better outcome of soft tissue healing under the influence of bromelain. proteolytic, anti-bacterial, anti-inflammatory, and anti-oedematogenic effects account for this improvement on the systemic level. it remains unknown, whether involved tissue cells are directly influenced by bromelain. in order to gain more insight into those mechanisms by which brome ... | 2013 | 23771413 |
| the influence of total solids content and initial ph on batch biohydrogen production by solid substrate fermentation of agroindustrial wastes. | hydrogen is a valuable clean energy source, and its production by biological processes is attractive and environmentally sound and friendly. in méxico 5 million tons/yr of agroindustrial wastes are generated; these residues are rich in fermentable organic matter that can be used for hydrogen production. on the other hand, batch, intermittently vented, solid substrate fermentation of organic waste has attracted interest in the last 10 years. thus the objective of our work was to determine the eff ... | 2013 | 23732191 |
| is pomegranate juice a potential perpetrator of clinical drug-drug interactions? review of the in vitro, preclinical and clinical evidence. | the area of fruit juice-drug interaction has received wide attention with numerous scientific and clinical investigations performed and reported for scores of drugs metabolized by cyp3a4/cyp2c9. while grapefruit juice has been extensively studied with respect to its drug-drug interaction potential, numerous other fruit juices such as cranberry juice, orange juice, grape juice, pineapple juice and pomegranate juice have also been investigated for its potential to show drug-drug interaction of any ... | 2013 | 23673492 |
| pineapple juice ingestion for gastric discomfort in diabetic gastroparesis. | | 2013 | 23650790 |
| production of laccase and manganese peroxidase by pleurotus pulmonarius in solid-state cultures and application in dye decolorization. | the production of ligninolytic enzymes (laccase and mn-dependent peroxidase) by the white-rot fungus pleurotus pulmonarius (fr.) quélet was studied in solid-state cultures using agricultural and food wastes as substrate. the highest activities of laccase were found in wheat bran (2,860 ± 250 u/l), pineapple peel (2,450 ± 230 u/l), and orange bagasse (2,100 ± 270 u/l) cultures, all of them at an initial moisture level of 85 %. the highest activities of mn peroxidase were obtained in pineapple pee ... | 2013 | 23645502 |
| zinc absorption in brazilian subjects fed a healthy meal. | the high amounts of phytic acid present in diets from developing countries are considered as important inhibitors of zinc (zn) absorption. the present study aimed to assess the fractional absorption of zn from a meal containing common brazilian foods using the stable isotope technique. | 2014 | 23634993 |
| cytotoxic effects of bromelain in human gastrointestinal carcinoma cell lines (mkn45, kato-iii, ht29-5f12, and ht29-5m21). | bromelain is a pineapple stem extract with a variety of therapeutic benefits arising from interaction with a number of different biological processes. several preclinical studies and anecdotal clinical observations have reported the anticancer properties of bromelain. in the present study, we investigated the cytotoxic effects of bromelain in four human cancer cell lines of gastrointestinal origin and the mechanisms involved. | 2013 | 23620673 |
| diversity of fusarium species and mycotoxins contaminating pineapple. | pineapple (ananas comosus var. comosus) is an important perennial crop in tropical and subtropical areas. it may be infected by various fusarium species, contaminating the plant material with mycotoxins. the aim of this study was to evaluate fusarium species variability among the genotypes isolated from pineapple fruits displaying fungal infection symptoms and to evaluate their mycotoxigenic abilities. forty-four isolates of ten fusarium species were obtained from pineapple fruit samples: f. ana ... | 2013 | 23572446 |
| structural characterization of a glucuronoarabinoxylan from pineapple (ananas comosus (l.) merrill) gum exudate. | native polysaccharide from pineapple gum (panp) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. it was characterized as a glucuronoarabinoxylan using nmr, methylation data, controlled smith degradation, carboxy-reduction, and esi-ms of oligosaccharides produced on mild acid hydrolysis of panp. hspec-malls-ri of carboxy-reduced fraction showed homogeneous profile (mw 1.943×10(5) g/mol). panp was composed of ara, xyl, gal, and glcpa (40:23:7:30 mo ... | 2013 | 23544593 |
| in vitro activity of pineapple extracts (ananas comosus, bromeliaceae) on rhipicephalus (boophilus) microplus (acari: ixodidae). | measures to control the cattle tick, rhipicephalus (boophilus) microplus, based only on chemical products are becoming unsustainable, mainly because of the development of resistance. the objective of this study was to test the effect of the aqueous extract of pineapple skin (aeps) and bromelain extracted from the stem (sigma-aldrich®, b4882) on engorged females and larvae of r. (b.) microplus in vitro. these substances were diluted in water and evaluated at eight concentrations. engorged females ... | 2013 | 23541882 |
| the concept of the eudicot shoot apical meristem as it applies to four spiraea (rosaceae), one mentha (lamiaceae) and one euonymus (celastraceae) cultivars based on chimeric analysis. | eversporting eudicots were sought to see if they behave like gymnosperms. behaviour of eversporting gymnosperm chimeras indicates a single apical cell is present in sam and it would be of interest to see if eudicot chimeras have the same behaviour. | 2013 | 23482330 |
| physicochemical and antioxidant properties of malaysian honeys produced by apis cerana, apis dorsata and apis mellifera. | the aim of the present study was to evaluate the physicochemical and antioxidant properties of malaysian monofloral honey samples-acacia, pineapple and borneo honey-and compare them with tualang honey. acacia and pineapple honey are produced by apis mellifera bees while borneo and tualang honey are produced by apis cerana and apis dorsata bees, respectively. | 2013 | 23433009 |
| reverse zymography alone does not confirm presence of a protease inhibitor. | reverse zymography is applied for identification and semi-quantification of protease inhibitors that are of protein in nature. however, a protein that shows band in reverse zymography against a protease used for digestion of the gel need not be an inhibitor; it might be resistant to degradation by the protease. we demonstrate that in reverse zymography, avidin, streptavidin and the leaf extract of catharanthus roseus behave like inhibitors of proteases like papain, ficin, bromelain extracts from ... | 2013 | 23417772 |
| changes in vitamin c, phenolic, and carotenoid profiles throughout in vitro gastrointestinal digestion of a blended fruit juice. | the aim of this research was to evaluate the influence of an in vitro gastrointestinal digestion on the stability and bioaccessibility of vitamin c, phenolic compounds, and carotenoids, as well as the antioxidant activity in a blended fruit juice (bfj) containing orange, pineapple, and kiwi. vitamin c and most of the analyzed phenolic compounds were quite stable under gastric conditions (recovery > 75%), whereas carotenoids diminished significantly (to 64%). the concentration of all the evaluate ... | 2013 | 23374081 |
| anti-inflammatory activity of bromelia hieronymi: comparison with bromelain. | some plant proteases (e. g., papain, bromelain, ficin) have been used as anti-inflammatory agents for some years, and especially bromelain is still being used as alternative and/or complementary therapy to glucocorticoids, nonsteroidal antirheumatics, and immunomodulators. bromelain is an extract rich in cysteine endopeptidases obtained from ananas comosus. in this study the anti-inflammatory action of a partially purified extract of bromelia hieronymi fruits, whose main components are cysteine ... | 2013 | 23364884 |
| dna profiling of pineapple cultivars in japan discriminated by ssr markers. | we developed 18 polymorphic simple sequence repeat (ssr) markers in pineapple (ananas comosus) by using genomic libraries enriched for ga and ca motifs. the markers were used to genotype 31 pineapple accessions, including seven cultivars and 11 breeding lines from okinawa prefecture, 12 foreign accessions and one from a related species. these ssr loci were highly polymorphic: the 31 accessions contained three to seven alleles per locus, with an average of 4.1. the values of expected heterozygosi ... | 2012 | 23341750 |
| properties and therapeutic application of bromelain: a review. | bromelain belongs to a group of protein digesting enzymes obtained commercially from the fruit or stem of pineapple. fruit bromelain and stem bromelainare prepared differently and they contain different enzymatic composition. "bromelain" refers usually to the "stem bromelain." bromelain is a mixture of different thiol endopeptidases and other components like phosphatase, glucosidase, peroxidase, cellulase, escharase, and several protease inhibitors. in vitro and in vivo studies demonstrate that ... | 2012 | 23304525 |
| relevance of the mention of antioxidant properties in yogurt labels: in vitro evaluation and chromatographic analysis. | the purpose of the inclusion of fruit (natural additives) in yogurt aims to increase its antioxidant activity and functionality. herein, a comparative study of the antioxidant potential of yogurts with pieces of various fruits was performed, including yogurts with mention of antioxidant properties in the label. free radicals scavenging activity, reducing power and inhibition of lipid peroxidation were evaluated by in vitro assays, as were the contents in antioxidants such as phenolics, flavonoid ... | 2013 | 26787624 |
| pineapple translation factor sui1 and ribosomal protein l36 promoters drive constitutive transgene expression patterns in arabidopsis thaliana. | the availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. in this study, the promoter and 5' untranslated regions of the evolutionally conserved protein translation factor sui1 gene and ribosomal protein l36 gene were isolated from pineapple and sequenced. each promoter was translationally fused to the gus reporter gene and transformed into the heterologous plant system arabidopsi ... | 2013 | 23263857 |
| dietary intake of melatonin from tropical fruit altered urinary excretion of 6-sulfatoxymelatonin in healthy volunteers. | this study assessed the melatonin content of six tropical fruits and examined whether human consumption could contribute to dietary melatonin as measured by 6-sulfatoxymelatonin (amt6-s, a marker of circulating melatonin in the body). melatonin was extracted using methanol and analyzed by high-performance liquid chromatography. in a clinical crossover study, 30 healthy volunteers consumed selected fruits one at a time, with a 1week wash-out period between fruits, until completing all six fruits. ... | 2013 | 23252791 |
| microarray analysis of gene expression profiles in ripening pineapple fruits. | pineapple (ananas comosus) is a tropical fruit crop of significant commercial importance. although the physiological changes that occur during pineapple fruit development have been well characterized, little is known about the molecular events that occur during the fruit ripening process. understanding the molecular basis of pineapple fruit ripening will aid the development of new varieties via molecular breeding or genetic modification. in this study we developed a 9277 element pineapple microa ... | 2012 | 23245313 |
| inhibitory effects of a kunitz-type inhibitor from pithecellobium dumosum (benth) seeds against insect-pests' digestive proteinases. | pithecellobium dumosum is a tree belonging to the mimosoideae subfamily that presents various previously characterized kunitz-type inhibitors. the present study provides a novel kunitz-trypsin inhibitor isoform purified from p. dumosum seeds. purification procedure was performed by tca precipitation followed by a trypsin-sepharose chromatography and a further reversed-phase hplc. purified inhibitor (pdki-4) showed enhanced inhibitory activity against bovine trypsin and chymotrypsin. furthermore, ... | 2013 | 23238511 |
| anti-inflammatory properties of fruit juices enriched with pine bark extract in an in vitro model of inflamed human intestinal epithelium: the effect of gastrointestinal digestion. | enrichment of fruit juices with pine bark extract (pbe) could be a strategy to compensate for phenolic losses during the gastrointestinal digestion. a coculture system with caco-2 cells and raw 264.7 macrophages was established as an in vitro model of inflamed human intestinal epithelium for evaluating the anti-inflammatory capacity of fruit juices enriched with pbe (0.5 g l(-1)) before and after in vitro digestion. the digestion of both pbe-enriched pineapple and red fruit juice led to signific ... | 2013 | 23220608 |
| synthesis, characterization and adsorptive properties of carbon with iron nanoparticles and iron carbide for the removal of as(v) from water. | this manuscript presents the synthesis of carbon modified with iron nanoparticles (cfe) and iron carbide (carfe) from the pyrolyzed crown leaves of pineapple (ananas comosus) treated with iron salts. the materials that were obtained were used for the removal of as(v) from aqueous media. the carbonaceous materials were characterized by scanning electron microscopy (sem), transmission electron microscopy (tem), x-ray diffraction (xrd), x-ray photoelectron spectroscopy (xps) and mossbauer spectrosc ... | 2013 | 23201599 |
| agrobacterium-mediated genetic transformation of pineapple (ananas comosus l., merr.). | pineapple (ananas comosus l., merr.) is a commercially important crop, grown in the tropical and subtropical regions. however, the crop is faced with postharvest damage and poor varietal and nutritional improvement. being a vegetatively propagated crop, conventional breeding programs take longer time for genetic improvement, which may not necessarily successfully develop an improved cultivar. hence, the genetic modification of pineapple is an alternative handy approach to improve pineapple. we h ... | 2013 | 23179718 |
| food allergy in africa: myth or reality? | food allergy has been traditionally perceived as being rare in africa. however, the prevalence of other allergic manifestations such as asthma and atopic dermatitis continue to rise in the higher-income african countries. since the food allergy epidemic in westernized countries has lagged behind that of allergic respiratory conditions, we hypothesize that food allergy is increasing in africa. this article systematically reviews the evidence for food allergy in africa, obtained through searching ... | 2014 | 23179518 |
| headspace solid-phase microextraction gas chromatography-mass spectrometry and gas chromatography-olfactometry analysis of volatile compounds in pineapple breads. | sensorial analysis of pineapple breads (conventionally baked, cpb; fully baked frozen, fpb and partially baked, ppb) showed no significant differences in terms of aroma and taste. on the contrary, the scores for the overall quality between the partially baked and conventionally baked breads showed significant (p < 0.05) differences. at the same time, headspace analysis using a solid-phase microextraction (spme) method identified 59 volatile compounds. the results of the aroma extracts dilution a ... | 2012 | 23174897 |
| design development scopes towards occupational wellness of women workers: specific reference to local agro based food processing industries in ne india. | women workers constitute one of the most vulnerable segments of the country's labour force. they often face different workplace health challenges than men do. they are engaged in a range of work that extends from heavy, monotonous, repetitive jobs, which are in many times experienced with low-paid and involves in long hours of work. women's workplace health problems are frequently compounded by getting more of the same at home--the "double jeopardy" of domestic work. specific issues to improve t ... | 2012 | 23151732 |
| solution structure of a phytocystatin from ananas comosus and its molecular interaction with papain. | the structure of a recombinant pineapple cystatin (accys) was determined by nmr with the rmsd of backbone and heavy atoms of twenty lowest energy structures of 0.56 and 1.11 å, respectively. it reveals an unstructured n-terminal extension and a compact inhibitory domain comprising a four-stranded antiparallel β-sheet wrapped around a central α-helix. the three structural motifs (g(45), q(89)xvxg, and w(120)) putatively responsible for the interaction with papain-like proteases are located in one ... | 2012 | 23139757 |
| serum melatonin levels and antioxidant capacities after consumption of pineapple, orange, or banana by healthy male volunteers. | melatonin is a naturally occurring molecule biosynthesized by the pineal gland of vertebrates; it also has been identified in many plants. it is considered an important antioxidant and may retard the development of some neurodegenerative diseases and cancer. previous studies in humans have measured melatonin metabolites in urine and have indicated that melatonin-containing foods may provide dietary melatonin. this study tested whether the consumption of fruits or fruit juice containing melatonin ... | 2013 | 23137025 |
| rna interference of endochitinases in the sugarcane endophyte trichoderma virens 223 reduces its fitness as a biocontrol agent of pineapple disease. | the sugarcane root endophyte trichoderma virens 223 holds enormous potential as a sustainable alternative to chemical pesticides in the control of sugarcane diseases. its efficacy as a biocontrol agent is thought to be associated with its production of chitinase enzymes, including n-acetyl-ß-d-glucosaminidases, chitobiosidases and endochitinases. we used targeted gene deletion and rna-dependent gene silencing strategies to disrupt n-acetyl-ß-d-glucosaminidase and endochitinase activities of the ... | 2012 | 23110120 |
| de novo assembly, characterization and functional annotation of pineapple fruit transcriptome through massively parallel sequencing. | pineapple (ananas comosus var. comosus), is an important tropical non-climacteric fruit with high commercial potential. understanding the mechanism and processes underlying fruit ripening would enable scientists to enhance the improvement of quality traits such as, flavor, texture, appearance and fruit sweetness. although, the pineapple is an important fruit, there is insufficient transcriptomic or genomic information that is available in public databases. application of high throughput transcri ... | 2012 | 23091603 |
| lc-ms/ms identification of a bromelain peptide biomarker from ananas comosus merr. | bromelain (br) is a cysteine peptidase (genbank aeh26024.1) from pineapple, with over 40 years of clinical use. the constituents mediating its anti-inflammatory activity are not thoroughly characterized and no peptide biomarker exists. our objective is to characterize br raw material and identify peptides in the plasma of br treated mice. after sds-page in-gel digestion, br (vn#3507; middletown, ct, usa) peptides were analyzed via lc/ms/ms using 95% protein probability, 95% peptide probability, ... | 2012 | 23082082 |
| environmental hazards of pesticides from pineapple crop production in the río jiménez watershed (caribbean coast, costa rica). | this study aimed to characterize environmental hazards of pesticides from pineapple production in riparian communities along the jiménez river watershed. to achieve our objectives riparian ecological quality indices on riparian habitat and macroinvertebrate assemblages were combined with toxicity assays, fish biomarkers, physico-chemical water analysis and pesticide environmental hazards. during two consecutive years and two periods (july and october), three reference and four impacted sites wer ... | 2012 | 23040047 |
| an analysis of consumer demand for fruits in sri lanka. 1981-2010. | micronutrient deficiency has become a serious health concern in many countries and sri lanka is no exception. inclusion of vegetables and fruits, which are rich in micronutrients, in the diet is considered as one of the most cost effective measures to alleviate such deficiencies. the purpose of this paper is to analyze consumer demand for fruits in sri lanka. the specific objectives are to: (i) describe the patterns of fruit consumption across various households groups (ii) document the changes ... | 2013 | 23036284 |
| assessing the effect of pineapple extract alone and in combination with vancomycin on streptococcus sanguis. | periodontitis is a major problem that affects a large number of patients in the society. various treatment alternatives have been proposed to control this pathologic condition. streptococcus sanguis is one of the countless pathogens involved in periodontitis. | 2012 | 24624172 |
| pineapple stem bromelain immobilized on different supports: catalytic properties in model wine. | bromelain from pineapple stem has been covalently immobilized on different supports to select the more efficient biocatalyst that should be applied toward unstable proteins in real white wine. in this preliminary study, catalytic properties of different immobilized bromelain forms were compared under wine-like conditions, against a synthetic substrate (bz-phe-val-arg-pna).covalent immobilization affected protease kinetic properties, even if all immobilized forms presented both a better substrate ... | 2012 | 23011793 |
| replacement levels of elephant grass by moist pineapple by-product silage in diets of santa inês crossbred sheep: performance and digestibility. | the present study evaluated the effect of replacing elephant grass (eg) with moist pineapple by-product silage (ps) on the apparent digestibility, consumption of digestible nutrients and performance of 25 castrated male lambs santa ines crossbreds. the lambs had an initial body weight of 20.2 ± 3.5 kg and were housed in individual pens in a completely randomised design with five treatments (replacement of eg by ps at five proportions of 0, 25, 50, 75 and 100 %) and five replicates during 74 days ... | 2013 | 23001582 |
| detection of postharvest changes of ascorbic acid in fresh-cut melon, kiwi, and pineapple, by using a low cost telemetric system. | the present paper deals with a novel telemetric device combined with a carbon amperometric sensor system to determine postharvest changes of ascorbic acid (aa) in fresh-cut fruits, without displacing products out of the storage rooms. the investigation was performed on kiwi, pineapple and melon, subjected to minimal processing, packaging, cold storage, and simulated shelf life. results demonstrated that aa content of fresh-cut fruits of all species declines differently during storage. cold stora ... | 2012 | 22953893 |
| chemical, technological and in vitro antioxidant properties of mango, guava, pineapple and passion fruit dietary fibre concentrate. | the aim of this work was to determine the chemical, technological and in vitro antioxidant properties of co-products from the industrialisation of some tropical exotic fruits, such as mango, pineapple, guava and passion fruit, and to evaluate their potential use as dietary fibre sources for food enrichment. proximate compositions were determined, as well as the total, insoluble and soluble fibre contents. the water holding, oil holding and swelling capacities were also determined. for the antiox ... | 2012 | 22953888 |
| expression patterns, activities and carbohydrate-metabolizing regulation of sucrose phosphate synthase, sucrose synthase and neutral invertase in pineapple fruit during development and ripening. | differences in carbohydrate contents and metabolizing-enzyme activities were monitored in apical, medial, basal and core sections of pineapple (ananas comosus cv. comte de paris) during fruit development and ripening. fructose and glucose of various sections in nearly equal amounts were the predominant sugars in the fruitlets, and had obvious differences until the fruit matured. the large rise of sucrose/hexose was accompanied by dramatic changes in sucrose phosphate synthase (sps) and sucrose s ... | 2012 | 22949808 |
| phenazepam and cannabinomimetics sold as herbal highs in new zealand. | a smokeable product called kronic, is legally available, sold under five product names, and marketed in new zealand as containing natural extracts. two such products called pineapple express and purple haze were purchased from shops in auckland city. they were investigated for the presence of synthetic drugs specifically synthesized for recreational purposes. the synthetic cannabinomimetics identified were jwh-018, jwh-073, jwh-122, jwh-250, and 1-pentyl-3-(4-methoxybenzoyl)indol. a compound not ... | 2012 | 22924168 |
| a study of lip print pattern in goan dental students - a digital approach. | to find the incidence of different types of lip patterns, the dominant pattern, quadrant wise, amongst the goan population. to assess, the quadrant wise differences in lip patterns among males and females and to report new lip print pattern in goan population. | 2012 | 22920761 |
| bromelain limits airway inflammation in an ovalbumin-induced murine model of established asthma. | allergic asthma continues to increase despite new pharmacological advances for both acute treatment and chronic-disease management. asthma is a multifactorial disease process with genetic, allergic, infectious, environmental, and dietary origins. researchers are investigating the benefits of lifestyle changes and alternative asthma treatments, including the ability of bromelain to inhibit inflammation. bromelain is a commonly used, proteolytically active pineapple extract. | 2015 | 22894886 |
| effect of mr contrast agents on quantitative accuracy of pet in combined whole-body pet/mr imaging. | clinical pet/mr acquisition protocols entail the use of mr contrast agents (mrca) that could potentially affect pet quantification following mr-based attenuation correction (ac). we assessed the effect of oral and intravenous (iv) mrca on pet quantification in pet/mr imaging. | 2012 | 22890801 |
| heavy metals health risk assessment for population via consumption of food crops and fruits in owerri, south eastern, nigeria. | this study assessed lead, cadmium, and nickel level in food crops, fruits and soil samples from ohaji and umuagwo and owerri in south eastern nigeria and estimated the potential health risks of metals. samples were washed, oven-dried at 70-80°c for 24 h and powdered. samples were digested with perchloric acid and nitric acid. metals were analysed with unicam atomic absorption spectrophotometer. | 2012 | 22853175 |
| aroma volatile compounds from two fresh pineapple varieties in china. | volatile compounds from two pineapples varieties (tainong no.4 and no.6) were isolated by headspace solid phase microextraction (hs-spme) and identified and quantified by gas chromatography-mass spectrometry (gc/ms). in the tainong no. 4 and no. 6 pineapples, a total of 11 and 28 volatile compounds were identified according to their retention time on capillary columns and their mass spectra, and quantified with total concentrations of 1080.44 μg·kg(-1) and 380.66 μg·kg(-1) in the tainong no.4 an ... | 2012 | 22837701 |
| palatability, digestibility and emotional pattern in 60 healthy volunteers after ingestion of an iced dessert presented in four different flavours: a subjective evaluation. | several variables lead to changes in human and animal eating behaviour and food choices. a pivotal role is played by food palatability, represented by food, smell, taste, texture, appearance and temperature. the aim of our study is to assess the potential differences in palatability and digestibility of four different flavoured iced desserts, consumed at the end of a standardized meal, and their impact on the emotional status of 60 healthy volunteers. sixty healthy volunteers, after ent and psyc ... | 2015 | 22824751 |
| development and optimisation by means of sensory analysis of new beverages based on different fruit juices and sherry wine vinegar. | despite the long history of sherry wine vinegar, new alternatives of consumption are being developed, with the aim of diversifying its market. several new acetic-based fruit juices have been developed by optimising the amount of sherry wine vinegar added to different fruit juices: apple, peach, orange and pineapple. once the concentrations of wine vinegar were optimised by an expert panel, the aforementioned new acetic fruit juices were tasted by 86 consumers. three different aspects were taken ... | 2013 | 22806566 |
| light and heavy dansyl reporter groups in food chemistry: amino acid assay in beverages. | 5-dimethylamino-1-sulfonyl naphthalene (dns, commonly referred as dansyl) is a functionality, bearing well-established properties in directing the fragmentation, by mass spectrometry (ms), of the corresponding ionized sulfonylated derivatives. this property is shared also by its labeled analogs. the use of d(0)/d(6) dns derivatives is now exploited in the application of the well-established isotope dilution mass spectrometric approach in the assay of complex mixtures. a new method for the quanti ... | 2012 | 22791261 |
| effects of fibre type and kefir, wine lemon, and pineapple marinades on texture and sensory properties of wild boar and deer longissimus muscle. | fibre type percentage and changes in textural parameters, sensory properties as well as mean fibre cross sectional area (csa), fibre shape, endomysium and perimysium thickness of wild boar and deer longissimus (l) muscle subjected to ageing with kefir, dry red wine, lemon and pineapple juice marinades for 4 days were studied. among the non-marinated and non-aged samples of muscles it was found that wild boar meat with its higher percentage of red fibres, higher csa, thicker connective tissue as ... | 2012 | 22766562 |
| bromelain purification through unconventional aqueous two-phase system (peg/ammonium sulphate). | this paper focuses on the feasibility of unconventional aqueous two-phase systems for bromelain purification from pineapple processing waste. the main difference in comparison with conventional systems is the integration of the liquid-liquid extraction technique with fractional precipitation, which can decrease the protein content with no loss of biological activity by removing of unwanted molecules. the analysis of the results was based on the response surface methodology and revealed that the ... | 2013 | 22763776 |
| study on the effect of chain-length compatibility of mixed anionic-cationic surfactants on the cloud-point extraction of selected organophosphorus pesticides. | the chain-length compatibility of mixed anionic-cationic surfactants was investigated for the extraction of organophosphorus pesticides (opps). cationic surfactants with different chain lengths (n = 12 and 16) were mixed with sodium dodecyl sulfate (sds; n = 12) for the mixed anionic-cationic surfactants-based extraction. six opps were studied including azinphos-methyl, parathion-methyl, fenitrothion, diazinon, chlorpyrifos, and prothiophos. reversed-phase high-performance liquid chromatography ... | 2012 | 22760506 |
| paenibacillus xylaniclasticus sp. nov., a xylanolytic-cellulolytic bacterium isolated from sludge in an anaerobic digester. | a mesophilic, facultative, anaerobic, xylanolytic-cellulolytic bacterium, tw1(t), was isolated from sludge in an anaerobic digester fed with pineapple waste. cells stained gram-positive, were spore-forming, and had the morphology of straight to slightly curved rods. growth was observed in the temperature range of 30 to 50°c (optimum 37°c) and the ph range of 6.0 to 7.5 (optimum ph 7.0) under aerobic and anaerobic conditions. the strain contained meso-diaminopimelic acid in the cell-wall peptidog ... | 2012 | 22752902 |
| bromelain enzyme from pineapple: in vitro activity study under different micropropagation conditions. | the aim of this work was to evaluate the activity of bromelain in pineapple plants (ananas comosus var. comosus), pérola cultivar, produced in vitro in different culture conditions. this enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. in this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. the most significant levels of bromelain ... | 2012 | 22736274 |
| isolation and characterization of a flowering locus t homolog from pineapple (ananas comosus (l.) merr). | flowering locus t (ft)-like genes are crucial regulators of flowering in angiosperms. a homolog of ft, designated as acft (genbank id: hq343233), was isolated from pineapple cultivar comte de paris by reverse transcriptase polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race). the cdna sequence of acft is 915 bp in length and contains an orf of 534 bp, which encodes a protein of 177 aa. molecular weight was 19.9 kda and isoelectric point was 6.96. the deduced protein seq ... | 2012 | 22710136 |
| anaerobic digestion of pineapple pulp and peel in a plug-flow reactor. | the objective of this research was to study the production of biogas by using pineapple pulp and peel, the by-products from fruit processing plants, in a plug-flow reactor (17.5 l total volume). the effects of feed concentration, total solids (ts) and hydraulic retention time (hrt) on degradation of the waste were investigated. the increase of pineapple pulp and peel of 2% (wt/vol) at hrt 7 d to 4% (wt/vol) at hrt 10 d showed increases in biogas production rate, biogas yield and methane yield - ... | 2012 | 22705859 |
| occupational exposure to pesticides and parkinson's disease: a systematic review and meta-analysis of cohort studies. | to systematically review available cohort studies and estimate quantitatively the association between occupational exposure to pesticides and parkinson's disease (pd). | 2012 | 22698719 |
| chemical shift assignments of the canecystatin-1 from saccharum officinarum. | cystatins are cysteine proteases inhibitors that are widely distributed among insects, mammalians and plants. here we report the complete resonance assignment of canecystatin-1 from saccharum officinarum obtained by heteronuclear multidimensional high-resolution nuclear magnetic resonance spectroscopy. the consensus chemical shift index was calculated and showed the presence of one α-helix (residues 27-43) and three β-strands (residues 48-74, 78-89 and 94-104), a secondary structure pattern that ... | 2013 | 22696137 |
| effects of wax treatment on the physiology and cellular structure of harvested pineapple during cold storage. | pineapple (ananas comosus (l.) merr. cv. 'paris') is sensitive to low temperature and highly susceptible to blackheart during cold storage, which causes serious fruit decay. this work investigated the effect of wax treatment (sta-fresh 2952, 60 g/l) on blackheart of pineapple under chilling stress. wax treatment significantly reduced blackheart symptoms after 14 days of storage and markedly delayed changes in firmness and flesh color during the whole period of storage. the weight loss of wax-tre ... | 2012 | 22681417 |
| extensive citrus triploid hybrid production by 2x×4x sexual hybridizations and parent-effect on the length of the juvenile phase. | the citrus fresh market demands the production of seedless citrus fruits, as seedy fruits are not accepted by consumers. the recovery of triploid plants has proven to be the most promising approach to achieve this goal, since triploids have very low fertility, are generally seedless and do not induce seeds in other cultivars by cross pollination. triploid plants can be recovered by 2x×4x sexual hybridization. in this work, we present an effective methodology to recover triploid plants from 2x×4x ... | 2012 | 22614256 |
| synergy between enge, xyna and mana from clostridium cellulovorans on corn stalk, grass and pineapple pulp substrates. | the synergistic interaction between various hemi/cellulolytic enzymes has become more important in order to achieve effective and optimal degradation of complex lignocellulose substrates for biofuel production. this study investigated the synergistic effect of three enzymes endoglucanase (enge), mannanase (mana) and xylanase (xyna) on the degradation of corn stalk, grass, and pineapple fruit pulp and determined the optimal degree of synergy between combinations of these enzymes. it was establish ... | 2011 | 22558536 |
| impact of six fruits--banana, guava, mangosteen, pineapple, ripe mango and ripe papaya--on murine hepatic cytochrome p450 activities. | the effects of six thai fruits, namely banana, guava, mangosteen, pineapple, ripe mango and ripe papaya, on cytochrome p450 (p450) activities were investigated. the median inhibitory concentrations (ic(50) ) of each of the fruit juices on cyp1a1, cyp1a2, cyp2e1 and cyp3a11 activities were determined. pineapple juice showed the strongest inhibitory effect against all the evaluated p450 isozyme activities in mouse hepatic microsomes, followed by mangosteen, guava, ripe mango, ripe papaya and banan ... | 2012 | 22499231 |
| characterization and expression analysis of acserk2, a somatic embryogenesis and stress resistance related gene in pineapple. | the isolation and characterization of acserk2, another somatic embryogenesis receptor-like kinase (serk) gene homolog, confirmed that there was a serk gene family in pineapple. acserk2 shared high similarity to acserk1 and other serks, containing all the characteristic domains of the serk family. the expressed recombinant protein of pet-acserk2 was soluble. acserk2 expression was effectively induced by 2,4-dichlorophenoxyacetic acid (2,4-d) and maintained in high level at the early stage of soma ... | 2012 | 22465534 |
| loss-of-function screen reveals novel regulators required for drosophila germline stem cell self-renewal. | the germline stem cells (gscs) of drosophila melanogaster ovary provide an excellent model system to study the molecular mechanisms of stem cell self-renewal. to reveal novel factors required for drosophila female gsc maintenance and/or division, we performed a loss-of-function screen in gscs by using a collection of p-element-induced alleles of essential genes. mutations in genes of various functional groups were identified to cause defects in gsc self-renewal. here we report that a group of mu ... | 2012 | 22413088 |