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alpha-tocopherol content in 62 edible tropical plants.vitamin e was determined by the high-performance liquid chromatography (hplc) method. all the plants tested showed differences in their alpha-tocopherol content and the differences were significant (p < 0.05). the highest alpha-tocopherol content was in sauropus androgynus leaves (426.8 mg/kg edible portion), followed by citrus hystrix leaves (398.3 mg/kg), calamus scipronum (193.8 mg/kg), starfruit leaves averrhoa belimbi (168.3 mg/kg), red pepper capsicum annum (155.4 mg/kg), local celery apiu ...200111410015
biguanide related compounds in traditional antidiabetic functional foods.biguanides such as metformin are widely used worldwide for the treatment of type-2 diabetes. the identification of guanidine and related compounds in french lilac plant (galega officinalis l.) led to the development of biguanides. despite of their plant origin, biguanides have not been reported in plants. the objective of this study was to quantify biguanide related compounds (brcs) in experimentally or clinically substantiated antidiabetic functional plant foods and potatoes. the corrected resu ...201323411283
biodepollution of wastewater containing phenolic compounds from leather industry by plant peroxidases.this study deals with the use of peroxidases (poxs) from allium sativum, ipomoea batatas, raphanus sativus and sorghum bicolor to catalyze the degradation of free phenolic compounds as well as phenolic compounds contained in wastewater from leather industry. secretory plant poxs were able to catalyze the oxidation of gallic acid, ferulic acid, 4-hydroxybenzoic acid, pyrogallol and 1,4-tyrosol prepared in ethanol 2% (v:v). efficiency of peroxidase catalysis depends strongly on the chemical nature ...201120803235
[simple preprocessing method for multi-determination of 235 pesticide residues in cooked ingredients of foods by gc/ms and lc/ms/ms].a simple preprocessing method was developed for multiresidue determination of pesticides in processed agricultural products. residues were extracted from homogenized samples with acetonitrile in a glass centrifuge tube, followed by salting-out and partitioning with n-hexane. co-extractives were removed by means of mini-column clean up. analysis was performed by gc/ms and lc/ms/ms. the prepared sample solutions were examined for matrix effects. matrix effects had both positive and negative effect ...200818633206
the trade in african medicinal plants in matonge-ixelles, brussels (belgium).maintaining cultural identity and preference to treat cultural bound ailments with herbal medicine are motivations for migrants to continue using medicinal plants from their home country after moving to europe and the usa. as it is generally easier to import exotic food than herbal medicine, migrants often shift to using species that double as food and medicine. this paper focuses on the trade in african medicinal plants in a congolese neighborhood in brussels (belgium). what african medicinal p ...201628179733
entomopathogenic potential of verticillium and acremonium species (deuteromycotina: hyphomycetes).hyphomycetes with conidia formed in slimy heads from hyaline mycelium were isolated from a range of insect hosts. isolation on artificial medium and microscopic examination revealed that these fungi in many cases were not verticillium lecanii despite a superficial resemblance to this common entomopathogen. the fungi were identified as verticillium fusisporum, verticillium psalliotae, verticillium lamellicola, and species of acremonium. isolates of these fungi were bioassayed against the sweet-po ...199910222186
dietary patterns and retinal vascular calibre in children and adolescents with type 1 diabetes.to examine the association between dietary patterns and retinal vascular calibre in children and adolescents with type 1 diabetes.201626749006
transmission of internal cork of sweet potato by the cotton aphid, aphis gossypii glover. 195817781179
a genome-wide bac-end sequence survey provides first insights into sweetpotato (ipomoea batatas (l.) lam.) genome composition.sweetpotato, ipomoea batatas (l.) lam., is an important food crop widely grown in the world. however, little is known about the genome of this species because it is a highly heterozygous hexaploid. gaining a more in-depth knowledge of sweetpotato genome is therefore necessary and imperative. in this study, the first bacterial artificial chromosome (bac) library of sweetpotato was constructed. clones from the bac library were end-sequenced and analyzed to provide genome-wide information about thi ...201627871234
structural insights into substrate specificity of feruloyl-coa 6'-hydroxylase from arabidopsis thaliana.coumarins belong to an important class of plant secondary metabolites. feruloyl-coa 6'-hydroxylase (f6'h), a 2-oxoglutarate dependent dioxygenase (2ogd), catalyzes a pivotal step in the biosynthesis of a simple coumarin scopoletin. in this study, we determined the 3-dimensional structure of the f6'h1 apo enzyme by x-ray crystallography. it is the first reported structure of a 2ogd enzyme involved in coumarin biosynthesis and closely resembles the structure of arabidopsis thaliana anthocyanidin s ...201525993561
elevated compartmentalization of na+ into vacuoles improves salt and cold stress tolerance in sweet potato (ipomoea batatas).salinity and low temperature are the main limiting factors for sweet potato (ipomoea batatas) growth and agricultural productivity. various studies have shown that plant nhx-type antiporter plays a crucial role in regulating plant tolerance to salt stress by intracellular na(+) compartmentalization. the arabidopsis thaliana atnhx1 gene that encodes a vacuolar na(+) /h(+) antiporter was introduced into the sweet potato cultivar xushu-22 by agrobacterium-mediated transformation to confer abiotic s ...201525307930
transcriptome analysis to identify putative floral-specific genes and flowering regulatory-related genes of sweet potato.sweet potato flowers were collected for a transcriptome analysis to identify the putative floral-specific and flowering regulatory-related genes by using the rna-sequencing technique. pair-end short reads were de novo assembled by an integrated strategy, and then the floral transcriptome was carefully compared with several published vegetative transcriptomes. a total of 2595 putative floral-specific and 2928 putative vegetative-specific transcripts were detected. we also identified a large numbe ...201324200775
role of arabidopsis a-fifteen in regulating leaf senescence involves response to reactive oxygen species and is dependent on ethylene insensitive2.leaf senescence is a highly regulated developmental process that is coordinated by several factors. many senescence-associated genes (sags) have been identified, but their roles during senescence remain unclear. a sweet potato (ipomoea batatas) sag, named spa15, whose function was unknown, was identified previously. to understand the role of spa15 in leaf senescence further, the orthologue of spa15 in arabidopsis thaliana was identified and characterized, and it was named arabidopsis a-fifteen ( ...201221940719
an arsenate reductase homologue possessing phosphatase activity from sweet potato (ipomoea batatas [l.] lam): kinetic studies and characterization.a cdna encoding a putative arsenate reductase homologue (ibarsr) was cloned from sweet potato (ib). the deduced protein showed a high level of sequence homology (16-66%) with arsrs from other organisms. a 3-d homology structure was created based on atarsr (pdb code 1t3k ) from arabidopsis thaliana. the putative active site of protein tyrosine phosphatase (hc(x)(5)r) is conserved in all reported arsrs. ibarsr was overexpressed and purified. the monomeric nature of the enzyme was confirmed by 15% ...201121388125
protective effect of supplemental anthocyanins on arabidopsis leaves under high light.ten anthocyanin components have been detected in roots of purple sweet potato (ipomoea batatas lam.) by high-performance liquid chromatography coupled to diode array detection and electrospray ionization tandem mass spectrometry. all the anthocyanins were exclusively cyanidins or peonidin 3-sophoroside-5-glucosides and their acylated derivatives. the total anthocyanin content in purple sweet potato powder obtained by solid-phase extraction was 66 mg g(-1). a strong capacity of purple sweet potat ...201019947975
activation tagging of a gene for a protein with novel class of cct-domain activates expression of a subset of sugar-inducible genes in arabidopsis thaliana.in the current studies, we examined sugar-inducible gene expression using the arabidopsis thaliana line sgsl, which carries luciferase (luc) and beta-glucuronidase (gus) reporter genes under the control of a 210-bp promoter derived from the sweet potato sporamin gene (spo(min)). we isolated an enhancer activation-tagged mutant of this line that showed high-level expression of luc and gus under non-inducing low-sugar conditions. the activator ofspo(min)::luc2 (asml2) gene located close to the enh ...200515960623
activator of spomin::luc1/wrinkled1 of arabidopsis thaliana transactivates sugar-inducible promoters.we isolated an enhancer activation-tagged mutant of arabidopsis thaliana line sgsl carrying the luciferase (luc) gene under control of a short sugar-inducible promoter derived from a sweet potato sporamin gene (spomin) that showed high level expression of luc under non-inducing conditions. the activator of spomin::luc1 (asml1) gene located downstream of the enhancer encoded an apetala2 (ap2)-type ap2 domain protein, and this gene was shown recently to be responsible for the wrinkled1 mutation wh ...200515753106
analysis of genes developmentally regulated during storage root formation of sweet potato.to identify the genes involved in storage root formation of sweet potato (ipomoea batatas), we performed a simplified differential display analysis on adventitious roots at different developmental stages of the storage root. the expression patterns were confirmed by semiquantitative rt-pcr analyses. as a result, 10 genes were identified as being developmentally regulated and were named srf1-srf10. the expression of srf1, srf2, srf3, srf5, srf6, srf7, and srf9 increased during storage root format ...200515700424
sugar-dependent expression of the chs-a gene for chalcone synthase from petunia in transgenic arabidopsis.transgenic arabidopsis thaliana plants were constructed by introduction of a fusion of the gene for beta-glucuronidase (gus) to the chs-a gene, which is one of the two genes for chalcone synthase that are actively expressed in the floral organs of petunia. the expression of the fusion gene chs-a::gus was low in transgenic arabidopsis plantlets, but it was enhanced when plantlets or detached leaves were transferred to a medium that contained 0.3 molar sucrose, glucose, or fructose. no enhancement ...199116668565
glutathione s-transferase slgste1 in spodoptera litura may be associated with feeding adaptation of host plants.spodoptera litura is polyphagous pest insect and feeds on plants of more than 90 families. in this study the role of glutathione s-transferase epilson 1 (slgste1) in s. litura in detoxification was examined. this gene was up-regulated in the midgut of s. litura at the transcriptional and protein levels when the insect fed on brassica juncea or diet containing phytochemicals such as indole-3-carbinol and allyl-isothiocyanate that are metabolic products of sinigrin and glucobrassicin in b. juncea. ...201626631599
biocompatibility of sweetpotato and peanut in a hydroponic system.'georgia red' peanut (arachis hypogaea l.) and tu-82-155 sweetpotato [ipomoea batatas (l.) lam] were grown in monocultured or intercropped recirculating hydroponic systems in a greenhouse using the nutrient film technique (nft). the objective was to determine whether growth and subsequent yield would be affected by intercropping. treatments were sweetpotato monoculture (sp), peanut monoculture (pn), and sweetpotato and peanut grown in separate nft channels but sharing a common nutrient solution ...199811795324
identification of hydroxycinnamoylquinic acids of arnica flowers and burdock roots using a standardized lc-dad-esi/ms profiling method.a screening method using lc-dad-esi/ms was developed for the identification of common hydroxycinnamoylquinic acids based on direct comparison with standards. a complete standard set for mono-, di-, and tricaffeoylquinic isomers was assembled from commercially available standards, positively identified compounds in common plants (artichokes, asparagus, coffee bean, honeysuckle flowers, sweet potato, and vernonia amygdalina leaves) and chemically modified standards. four c18 reversed phase columns ...200818837557
enhanced chemiluminescence: a sensitive analytical system for detection of sweet potato peroxidase.3-(10'-phenothiazinyl)propane-1-sulfonate (sptz) was shown to be a potent enhancer of anionic sweet potato peroxidase (aspp)-induced chemiluminescence. the optimal conditions for aspp-catalyzed oxidation of luminol were investigated by varying the concentrations of luminol, hydrogen peroxide, tris, and sptz as well as the ph values of the reaction mixture. addition of 4-morpholinopyridine (morp) to the reaction mixture markedly increased the light intensity. using sptz and morp together enhanced ...201020449843
[luminol oxidation by hydrogen peroxide with chemiluminescent signal formation catalyzed by peroxygenase from the fungus agrocybe aegerita v.brig].conditions of luminol oxidation by hydrogen peroxide in the presence of peroxygenase from the mushroom agrocybe aegerita v.brig have been optimized. the ph value (8.8) at which fungal peroxygenase produces a maximum chemiluminescent signal has been shown to be similar to the ph optimum value of horseradish peroxidase. luminescence intensity changed when the concentration of tris buffer was varied; maximum intensity of chemiluminescence was observed in 40 mm solution. it has been shown that enhan ...201320198921
palm tree peroxidase-based biosensor with unique characteristics for hydrogen peroxide monitoring.three amperometric enzyme electrodes have been constructed by adsorbing anionic royal palm tree peroxidase (rptp), anionic sweet potato peroxidase (spp), or cationic horseradish peroxidase (hrp-c) on spectroscopic graphite electrodes. the resulting h(2)o(2)-sensitive biosensors were characterized both in a flow injection system and in batch mode to evaluate their main bioelectrochemical parameters, such as ph dependency, i(max), k(m)(app), detection limit, linear range, operational and storage s ...200516242613
cyclometalated ruthenium(ii) complexes as efficient redox mediators in peroxidase catalysis.cyclometalated ruthenium(ii) complexes, [ru(ii)(c~n)(n~n)(2)]pf(6) [hc~n=2-phenylpyridine (hphpy) or 2-(4'-tolyl)pyridine; n~n=2,2'-bipyridine, 1,10-phenanthroline, or 4,4'-dimethyl-2,2'-bipyridine], are rapidly oxidized by h(2)o(2) catalyzed by plant peroxidases to the corresponding ru(iii) species. the commercial isoenzyme c of horseradish peroxidase (hrp-c) and two recently purified peroxidases from sweet potato (spp) and royal palm tree (rptp) have been used. the most favorable conditions fo ...200312774217
bienzyme biosensors for glucose, ethanol and putrescine built on oxidase and sweet potato peroxidase.amperometric biosensors for glucose, ethanol, and biogenic amines (putrescine) were constructed using oxidase/peroxidase bienzyme systems. the h(2)o(2) produced by the oxidase in reaction with its substrate is converted into a measurable signal via a novel peroxidase purified from sweet potato peels. all developed biosensors are based on redox hydrogels formed of oxidases (glucose oxidase, alcohol oxidase, or amine oxidase) and the newly purified sweet potato peroxidase (spp) cross-linked to a r ...200312706582
daily consumption of indian spinach (basella alba) or sweet potatoes has a positive effect on total-body vitamin a stores in bangladeshi men.recent evidence suggests that the vitamin a equivalency of beta-carotene from plant sources is lower than previously estimated.200415321812
phylogenetic relationships among whiteflies in the bemisia tabaci (gennadius) species complex from major cassava growing areas in kenya.whiteflies, bemisia tabaci (gennadius) are major insect pests that affect many crops such as cassava, tomato, beans, cotton, cucurbits, potato, sweet potato, and ornamental crops. bemisia tabaci transmits viral diseases, namely cassava mosaic and cassava brown streak diseases, which are the main constraints to cassava production, causing huge losses to many small-scale farmers. the aim of this work was to determine the phylogenetic relationships among bemisia tabaci species in major cassava grow ...201728264479
non-invasive delivery of dsgst is lethal to the sweet potato whitefly, bemisia tabaci (g.) (hemiptera: aleyrodidae).the sweet potato whitefly, bemisia tabaci (g.) biotype b (hemiptera: aleyrodidae), is one of the most economically important pest, by being a dreaded vector of geminiviruses, and also causes direct damage to the crops by sucking phloem sap. glutathione s-transferase (gst) is a large family of multifunctional enzymes that play pivotal roles in the detoxification of secondary allelochemical produced by the host plants and in insecticide resistance, thus regulates insect growth and development. the ...201525480347
first report of bemisia tabaci mediterranean (q biotype) species in brazil.the whitefly bemisia tabaci is a major cosmopolitan pest and comprises a complex of more than 36 cryptic species that cause serious damage to agricultural crops worldwide. in this study, the mediterranean species of b. tabaci, formerly known as q biotype, was identified for the first time in brazil.201525212515
variations in the identity and complexity of endosymbiont combinations in whitefly hosts.the target of natural selection is suggested to be the holobiont - the organism together with its associated symbiotic microorganisms. the well-defined endosymbiotic communities of insects make them a useful model for exploring the role of symbiotic interactions in shaping the functional repertoire of plants and animals. here, we studied the variations in the symbiotic communities of the sweet potato whitefly bemisia tabaci (hemiptera: aleyrodidae) by compiling a dataset of over 2000 individuals ...201425071729
characteristics, phenotype, and transmission of wolbachia in the sweet potato whitefly, bemisia tabaci (hemiptera: aleyrodidae), and its parasitoid eretmocerus sp. nr. emiratus (hymenoptera: aphelinidae).wolbachia is a common intracellular bacterial endosymbiont of insects, causing a variety of effects including reproductive manipulations such as cytoplasmic incompatibility (ci). in this study, we characterized wolbachia in the whitefly bemisia tabaci and in the whitefly parasitoid eretmocerus sp. nr. emiratus. we also tested for horizontal transmission of wolbachia between and within trophic levels, and we determined the phenotype of wolbachia in e. sp. nr. emiratus. using multilocus sequence t ...201424763092
multilocus approach to clarify species status and the divergence history of the bemisia tabaci (hemiptera: aleyrodidae) species complex.the sweet potato whitefly, bemisia tabaci, is a highly differentiated species complex. despite consisting of several morphologically indistinguishable entities and frequent invasions on all continents with important associated economic losses, the phylogenetic relationships, species status, and evolutionary history of this species complex is still debated. we sequenced and analyzed one mitochondrial and three single-copy nuclear genes from 9 of the 12 genetic groups of b. tabaci and 5 closely re ...201424685497
ipomovirus--an atypical genus in the family potyviridae transmitted by whiteflies.ipomoviruses (genus ipomovirus) are whitefly-transmitted viruses assigned to the family potyviridae. they are characterised by filamentous flexible particles and a positive-sense single-stranded rna (+ssrna) genome. the viral genome is translated into a polyprotein precursor, which is processed into mature proteins and a short overlapping open reading frame. the genus ipomovirus contains four accepted species and one unapproved species, and two other tentative members have recently been characte ...201424464680
activity of flonicamid on the sweet potato whitely bemisia tabaci (homoptera: aleyrodidae) and its natural enemies.flonicamid is a novel systemic insecticide that acts as a feeding blocker with potential use against whiteflies within ipm control tactics. flonicamid efficacy against bemisia tabaci mediterranean populations from crete was examined, as well as side effects on selected beneficials used extensively in current ipm schemes.201424408346
spatial genetic heterogeneity in populations of a newly invasive whitefly in china revealed by a nation-wide field survey.even though introductions of exotic species provide ready-made experiments of rapid evolution, few studies have examined the genetic structure of an exotic species shortly after its initial introduction and subsequent spread. to determine the genetic structure of its populations during the initial introduction, we investigated the invasive sweet potato whitefly (bemisia tabaci q, commonly known as b. tabaci biotype q) in china, which was introduced in approximately 2003. a total of 619 b. tabaci ...201324302995
two cytochrome p450 genes are involved in imidacloprid resistance in field populations of the whitefly, bemisia tabaci, in china.the sweet potato whitefly, bemisia tabaci (gennadius) (hemiptera:aleyrodidae), is an invasive and damaging pest of field crops worldwide. the neonicotinoid insecticide imidacloprid has been widely used to control this pest. we assessed the species composition (b vs. q), imidacloprid resistance, and association between imidacloprid resistance and the expression of five p450 genes for 14-17 b. tabaci populations in 12 provinces in china. fifteen of 17 populations contained only b. tabaci q, and tw ...201324267696
use of fluorescence, a novel technique to determine reduction in bemisia tabaci (hemiptera: aleyrodidae) nymph feeding when exposed to benevia and other insecticides.the sweet potato whitefly, bemisia tabaci (gennadius), is an economically important pest in the united states and other countries. growers in many places rely on the use of insecticides to reduce populations of b. tabaci. however, insecticides may take a few days to cause b. tabaci mortality and some do not reduce feeding before death. earlier reduction of feeding of whiteflies would decrease the physiological effects on plants, reduce the production of sooty mold and potentially reduce the tran ...201323786044
investigating contact toxicity of geranium and artemisia essential oils on bemisia tabaci gen.sweet potato whitefly, bemisia tabaci gen. (b. tabaci), is one of the most important pests of various greenhouse crops in iran. nowadays, chemical insecticides are broadly used for control of the pests that causes risk to consumer's health. for the first time, contact toxicity of pelargonium roseum andrews and artemisia sieberi besser essential oils on b. tabaci and its possible application against the whitefly was evaluated in 2012.201325050264
genome sequences of the primary endosymbiont "candidatus portiera aleyrodidarum" in the whitefly bemisia tabaci b and q biotypes."candidatus portiera aleyrodidarum" is the obligate primary endosymbiotic bacterium of whiteflies, including the sweet potato whitefly bemisia tabaci, and provides essential nutrients to its host. here we report two complete genome sequences of this bacterium from the b and q biotypes of b. tabaci.201223144417
insecticidal activity of some reducing sugars against the sweet potato whitefly, bemisia tabaci, biotype b.the effects of 16 sugars (arabinose, cellobiose, fructose, galactose, gentiobiose, glucose, inositol, lactose, maltose, mannitol (a sugar alcohol), mannose, melibiose, ribose, sorbitol, trehalose, and xylose) on sweet potato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) survival were determined using in vitro bioassays. of these sugars, arabinose, mannose, ribose, and xylose were strongly inhibitory to both nymphal and adult survival. when 10% mannose was added to the nymphal diet ...201021268696
resistance of tomato genotypes to the greenhouse whitefly trialeurodes vaporariorum (west.) (hemiptera: aleyrodidae).the greenhouse whitefly, trialeurodes vaporariorum westwood, is the most common and abundant whitefly in argentine horticultural greenhouse crops, especially in tomato (solanum lycopersicum). resistance in some wild tomato relatives, such as s. peruvianum, s. habrochaites and s. pennellii to the greenhouse whitefly has been described. the mi gene confers effective resistance against several species of insects, among them the sweet potato whitefly, bemisia tabaci gennadius. resistance to t. vapor ...201021120390
[effects of host plants on selection behavior and biological parameters of bemisia tabaci gennadius biotype b].based on the observation of free diffusion in petri dish and biology, the selection behavior and biological parameters of bemisia tabaci gennadius biotype b on host plants cucumber, eggplant, pepper, cotton, and sweet potato were investigated. no difference was observed in the individual number of b. tabaci on the five host plant leaves during the early period of free diffusion (within 2 h), but changes were found within the following periods (4-48 h), i.e., the b. tabaci individuals increased o ...200920030150
aerial electrostatic-charged sprays for deposition and efficacy against sweet potato whitefly (bemisia tabaci) on cotton.the efficacy of aerial electrostatic-charged sprays was evaluated for spray deposit characteristics and season-long control of sweet potato whitefly (swf), bemisia tabaci genn. biotype b (aka b. argentifolii bellows & perring), in an irrigated 24 ha cotton field. treatments included electrostatic-charged sprays at full and half active ingredient (ai) label rate, uncharged sprays and conventional sprays applied with cp nozzles at full label rate with several different insecticides.200919291696
global relationships of bemisia tabaci (hemiptera: aleyrodidae) revealed using bayesian analysis of mitochondrial coi dna sequences.bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a species complex that is one of the most devastating agricultural pests worldwide and affects a broad range of food, fiber and ornamental crops. unfortunately, using parsimony and neighbor joining methods, global phylogenetic relationships of the major races/biotypes of b. tabaci remain unresolved. aside from the limitations of these methods, phylogenetic analyses have been limited to only small subsets of the global collection of b. tabaci ...200717627853
toxicity and feeding deterrence of crude toxin extracts of lecanicillium (verticillium) lecanii (hyphomycetes) against sweet potato whitefly, bemisia tabaci (homoptera: aleyrodidae).crude toxins [toxin(v3450) and toxin(vp28,) extracted from lecanicillium (verticillium) lecanii (zimmermann) gams & zare strain v3450 and vp28 respectively] were tested for contact toxicity, feeding deterrence and repellent activity against the sweet potato whitefly, bemisia tabaci (gennadius) (homoptera: aleyrodidae). both toxins showed ovicidal activity to eggs, with lc(50) values of 447 and 629 mg l(-1) respectively. nymphs of b. tabaci were the most susceptible stages (lc(50) values were cal ...200717315271
ecdysteroids and juvenile hormones of whiteflies, important insect vectors for plant viruses.ecdysteroids and juvenile hormones (jhs) regulate many physiological events throughout the insect life cycle, including molting, metamorphosis, ecdysis, diapause, reproduction, and behavior. fluctuation of whitefly ecdysteroid levels and the identity of the whitefly molting hormone (20-hydroxyecdysone) have only been reported within the last few years. an ecdysteroid commitment peak that is associated with the reprogramming of tissues for a metamorphic molt in many holometabolous and some hemime ...200717258230
tropical whitefly ipm project.the tropical whitefly ipm project (twfp) is an initiative of the systemwide ipm programme of the consultative group on international agricultural research (cgiar), financed by the department for international development (dfid) of the united kingdom, the danish international development agency (danida), the united states department of agriculture (usda) and agency for international development (usaid), the australian centre for international agricultural research (aciar), and the new zealand age ...200717222696
critical feeding periods for last instar nymphal and pharate adults of the whiteflies, trialeurodes vaporariorum and bemisia tabaci.a critical feeding period is the time after which 50% of a given species of insect can be removed from its food source and complete development by undergoing adult eclosion. the critical feeding period was determined for the greenhouse white fly, trialeurodes vaporariorum, and the sweet potato whitefly, bemisia tabaci (biotype b) (homptera/hemiptera: aleyrodidae). fourth (last) instar and pharate adult whiteflies were removed from green bean leaves, staged, placed on filter paper in small petri ...200720302459
host-parasitoid interactions relating to penetration of the whitefly, bemisia tabaci, by the parasitoid wasp, eretmocerus mundus.it has been reported that the aphelinid wasp eertmocerus mundus parasitizes all four nymphal instars of the sweet potato whitefly, bemisia tabaci (biotype b), with 3rd instars being the preferred hosts. the parasitoid lays its egg on the leaf underneath the host nymph. first instars hatch and later penetrate the whitefly. previous studies have shown that the initiation of parasitoid penetration induces the host to form a cellular capsule around the parasitoid. as described here, females never ov ...200517119628
within-plant distribution and sampling of single and mixed infestations of bemisia tabaci and trialeurodes vaporariorum (homoptera: aleyrodidae) in winter tomato crops.in several areas of spain, the greenhouse whitefly, trialeurodes vaporariorum (westwood), and the sweet potato whitefly, bemisia tabaci (gennadius), coexist in tomato, lycopersicon esculentum miller. for integrated pest management decision-making, it is important to know the abundance of each species, because they exhibit different abilities to transmit viruses, are susceptible to different biological control agents, and have different responses to insecticides. this study was conducted to provi ...200616686130
identification of the molting hormone of the sweet potato (bemisia tabaci) and greenhouse (trialeurodes vaporariorum) whitefly.in order to identify the whitefly molting hormone, whole body extracts of mature 4th instar and newly formed pharate adult bemisia tabaci (biotype b) and trialeurodes vaporariorum were prepared and subjected to reverse phase high performance liquid chromatography (rphplc). ecdysteroid content of fractions was determined by enzymeimmunoassay (eia). the only detectable ecdysteroids that were present in significant amounts in whitefly extracts were ecdysone and 20-hydroxyecdysone. the concentration ...200515686645
vitellin of the sweet potato whitefly, bemisia tabaci: biochemical characterization and titer changes in the adult.sds-page of the sweet potato whitefly (bemisia tabaci) egg extract showed one major band (approximately 190 kda) and two minor bands (approximately 75 kda and 67 kda). a distinct 190 kda band was also present in male extract. on sds gels the vitellin band of the greenhouse whitefly (trialeurodes vaporarium) was larger, about 220 kda. the native molecular mass of sweet potato whitefly vitellin was estimated to be 375 kda using 4-20% native porelimiting gel electrophoresis. its isoelectric point w ...19979041698
localized migration and dispersal by the sweet potato whitefly, bemisia tabaci.laboratory populations of the sweet potato whitefly, bemisia tabaci, have been shown to consist of both migratory and trivial flying morphs. the behavior of these forms as part of the process of short-range migration was examined under field conditions. insects were marked in a field of melons using fluorescent dust during two consecutive growing seasons. during the first growing season, passive traps used to collect living whiteflies, were placed along 16 equally spaced transects radiating from ...199628307104
high performance liquid chromatography analysis of carbohydrates of cotton-phloem sap and of honeydew produced by bemisia tabaci feeding on cotton.phloem sap from cotton (gossypium hirsutum l.) was collected from young and mature leaves by the aphid-stylet technique. exudate was analyzed for carbohydrates by hplc using sensitive pulsed amperometric detection. the predominant carbohydrate present (>90%) was identified as sucrose. a second, unidentified compound that was not one of the more commonly translocated sugars was detected in mature leaves. carbohydrates in honeydew produced by the sweet-potato whitefly (bemisia tabaci [genn.]) feed ...199216668706
interaction of a potential vacuolar targeting receptor with amino- and carboxyl-terminal targeting determinants.a protein of 80 kd from developing pea (pisum sativum) cotyledons has previously been shown to exhibit characteristics of a vacuolar targeting receptor by means of its affinity for the amino-terminal vacuolar targeting sequence of proaleurain from barley (hordeum vulgare). in this report we show that the same protein also binds to the amino-terminal targeting peptide of prosporamin from sweet potato (ipomoea batatas) and to the carboxyl-terminal targeting determinant of pro-2s albumin from brazi ...19968787025
development of extruded ready-to-eat (rte) snacks using corn, black gram, roots and tuber flour blends.extruded rte snacks were prepared from flour blends made with corn flour, bengal gram flour, roots and tuber flours in a proportion of 60-80: 20: 20 respectively and moisture was adjusted to 17-20 %. the roots and tubers flours were developed from potato (solanum tuberosum), yam (dioscorea spp.), sweet potato (ipomoea batatas l.), taro (colocassia esculenta) and beet root (beta vulgaris). different formulations were extruded at 80 ± 5 °c (heater i) and 95-105 °c (heater ii) temperature, 300-350  ...201425190848
refined avian risk assessment for chlorpyrifos in the united states.refined risk assessments for birds exposed to flowable and granular formulations ofcpy were conducted for a range of current use patterns in the united states. overall,the collective evidence from the modeling and field study lines of evidence indicate that flowable and granular cpy do not pose significant risks to the bird communities foraging in agro-ecosystems in the united states. the available information indicates that avian incidents resulting from the legal, registered uses of cpy have b ...201424723136
invertase proteinaceous inhibitor of cyphomandra betacea sendt fruits.this work describes a new invertase proteinaceous inhibitor from cyphomandra betacea sendt. (tomate de arbol) fruits. the proteinaceous inhibitor was isolated and purified from a cell wall preparation. the ph stability, kinetics of the inhibition of the c. betacea invertase, inhibition of several higher plant invertases and lectin nature of the inhibitor were studied. the inhibitor structure involves a single polypeptide (mr = 19000), as shown by gel filtration and sds-page determinations. n-ter ...200011140613
composition of suberin-associated waxes from the subterranean storage organs of seven plants : parsnip, carrot, rutabaga, turnip, red beet, sweet potato and potato.the waxes associated with the suberin in the periderm of the underground storage organs of parsnip (pastinaca sativa l.), carrot (daucus carota l.), rutabaga (brassica napobrassica mill.), turnip (brassica rapa l.), red beet (beta vulgaris l.), sweet potato (ipomoea batatas l.) and potato (solanum tuberosum l.) were isolated, fractionated into hydrocarbon, wax ester, free fatty alcohol and free fatty acid fractions, and analyzed by combined gas chromatography and mass spectrometry. the amount of ...198024310190
evidence for covalently attached p-coumaric acid and ferulic acid in cutins and suberins.p-coumaric acid (4-hydroxycinnamic acid) and ferulic acid (4-hydroxy-3-methoxycinnamic acid) have been identified as constituents of cutin. their reduction products were isolated from a phenolic fraction released from the cutin of the fruits of apple, peach, pear, and two varieties of tomato and apple leaf by treatment with lialh(4) or liald(4). they were identified by combined gas chromatography and mass spectrometry. p-coumaric acid was present in all samples of cutin (0.07-0.53% by weight), w ...197516659364
rooted leaves for physiological experiments.rooted leaves provide convenient material for investigating various physiological processes. details are given for producing rooted leaves of species with tuberous roots, e.g. sweet potato (ipomoea batatas) and dahlia (dahlia variabilis) and species with tap roots, e.g. sugar beet (beta vulgaris) and runner bean (phaseolus coccineus). the storage root formed on a single sugar-beet leaf develops concentric cambia suggesting that their formation is not determined by particular leaves.197224481792
oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling injury.arrhenius plots of the respiration rates of mitochondria isolated from chilling sensitive plant tissues (tomato and cucumber fruit, and sweet potato roots) showed a linear decrease from 25 c to about 9 to 12 c (with q(10) values of 1.3 to 1.6), at which point there was a marked deviation with an increased slope as temperatures were reduced to 1.5 c (q(10) of 2.2 to 6.3). the log of the respiration rate of mitochondria from chilling resistant tissues (cauliflower buds, potato tubers, and beet roo ...19705427108
safety assessment and detection method of genetically modified chinese kale (brassica oleracea cv. alboglabra ).sporamins are tuberous storage proteins and account for 80% of soluble protein in sweet potato tubers with trypsin-inhibitory activity. the expression of sporamin protein in transgenic chinese kale (line boa 3-1) conferred insecticidal activity toward corn earworm [ helicoverpa armigera (hubner)] in a previous report. in this study, we present a preliminary safety assessment of transgenic chinese kale boa 3-1. bioinformatic and simulated gastric fluid (sgf) analyses were performed to evaluate th ...200919216530
a hemolymph major anionic peptide, hemap, motivates feeding behavior in the sweetpotato hornworm, agrius convolvuli.we recently identified a novel feeding-modulating peptide, hemolymph major anionic peptide (hemap), designated bommo-hemap (b-hemap), from hemolymph of the silkworm bombyx mori. b-hemap has a unique biological activity in modulating the regular frequency of feeding motivation, which is accompanied by increased foraging behaviors. to confirm the conservation of the hemap-regulated feeding mechanism in lepidopteran species, we purified and sequenced two candidate peptides from the hemolymph of lar ...201222035292
bombyxin-related peptides: cdna structure and expression in the brain of the hornworm agrius convolvuli [corrected].we have cloned three cdnas from the sweet potato hornworm agrius convolvuli that encode precursor molecules for peptides structurally related to bombyxin, an insulin-related brain secretory peptide in bombyx mori. the agrius bombyxin-related peptide (abrp) cdnas are classified into type a and b according to their sequence similarity. the prepro-abrps deduced from the cdna sequences have the insulin-like domain organization of signal peptide/b chain/c peptide/a chain. the abrp transcripts in agri ...19968673077
effect of feeding sweet-potato condensed distillers solubles on intake and urinary excretion of minerals in japanese black steers.four japanese black steers (16 months of age) were assigned to a 4 × 4 latin square design to investigate the effect of graded levels of sweet-potato condensed distillers solubles (scds) in their diets on intake and urinary excretion of minerals. the four diets consisted of 0%, 10%, 20% and 30% (dry matter (dm) basis) scds, with scds replacing commercial concentrate (cc). intake of k, cl, s, p and mg increased linearly with increasing scds content. urinary ph increased linearly with increasing d ...201727071727
cocoa flavonoids attenuate high glucose-induced insulin signalling blockade and modulate glucose uptake and production in human hepg2 cells.insulin resistance is the primary characteristic of type 2 diabetes. cocoa and its main flavanol, (-)-epicatechin (ec), display some antidiabetic effects, but the mechanisms for their preventive activities related to glucose metabolism and insulin signalling in the liver remain largely unknown. in the present work, the preventive effect of ec and a cocoa polyphenolic extract (cpe) on insulin signalling and on both glucose production and uptake are studied in insulin-responsive human hepg2 cells ...201424262486
performance of lactating dairy cows fed a diet based on treated rice straw and supplemented with pelleted sweet potato vines.the purpose of this study was to evaluate the effects of sweet potato vine pellet (swepp) in concentrate diets on nutrient digestibility and rumen ecology in lactating dairy cows fed on urea-treated rice straw. three multiparous holstein crossbred cows in mid-lactation were randomly assigned according to a 3 × 3 latin square design, and the treatments were as follows: t1 = control (no supplementation), t2 = supplementation of sweet potato vine pellet with 50 g/kg urea (swepp i) at 300 g/head/day ...201322961296
new gene construction strategy in t-dna vector to enhance expression level of sweet potato sporamin and insect resistance in transgenic brassica oleracea.sporamin, an abundant storage protein in tuberous roots of sweet potato, possesses strong inhibitory activity against trypsin and pest-resistance. to promote consistent high-level expression of sporamin and insect resistance in transgenic brassica plants, a wound-responsive sporamin promoter (pspoa) alone or combined with matrix-attached-region-like dna segment (spomar) were constructed for driving sporamin cdna. the results showed the transgenic plants containing pspoa-drived sporamin and spoma ...200622980206
malate oxidation, rotenone-resistance, and alternative path activity in plant mitochondria.the effect of cyanide and rotenone on malate (ph 6.8), malate plus glutamate (ph 7.8), citrate, alpha-ketoglutarate, and succinate oxidation by cauliflower (brassica oleracea l.) bud, sweet potato (ipomoea batatis l.) tuber, and spinach (spinacia oleracea and kalanchoë daigremontiana leaf mitochondria was investigated. cyanide inhibited all substrates equally with the exception of malate plus glutamate; in this case, inhibition of o(2) uptake was more severe due to an effect of cyanide on aspart ...198216662651
trypsin-induced atpase activity in potato mitochondria.potato mitochondria (solanum tuberosum var. russet burbank), which readily phosphorylate adp in oxidative phosphorylation, show low levels of atpase activity which is stimulated neither by mg(2+), 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with triton x-100, individually or in concert. treatment of disrupted potato mitochondria with trypsin stimulates mg(2+)-dependent, oligomycin-sensitive atpase activity 10- to 15-fold, suggesting the pr ...197616659532
risk factors for renal cell carcinoma in a japanese population.the incidence of renal cell carcinoma (rcc) is high in western and northern europe and north america, and low in asia. although the incidence of rcc in japan is lower than the rates in the other industrialized countries, there is no doubt that it is increasing. in this paper, we would like to introduce the summary of findings of jacc study, which evaluate the risk factors for rcc in a japanese population. jacc study suggests nine risk factors (i.e., smoking, obesity, low physical activity, hyper ...201425422180
risk factors for renal cell cancer in a japanese population.the incidence of renal cell cancer has been increasing worldwide. although the incidence of renal cell cancer in japan is lower than the rates in the other industrialized countries, there is no doubt that it is increasing. in this paper, we would like to introduce the result of our studies, which evaluate the risk factors for renal cell cancer in japan. hypertension, diabetes mellitus, kidney diseases, fondness for fatty food and black tea showed an increased risk of renal cell carcinoma while a ...200920689612
risk factors for kidney cancer in a japanese population: findings from the jacc study.the incidence of kidney cancer is high in western and northern europe and north america, and low in asia. although the incidence of kidney cancer in japan is lower than the rates in the other industrialized countries, there is no doubt that it is increasing.200516127235
high-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity-reducing process.viscosity reduction has a great impact on the efficiency of ethanol production when using roots and tubers as feedstock. plant cell wall-degrading enzymes have been successfully applied to overcome the challenges posed by high viscosity. however, the changes in cell wall polymers during the viscosity-reducing process are poorly characterized. comprehensive microarray polymer profiling, which is a high-throughput microarray, was used for the first time to map changes in the cell wall polymers of ...201725757626
united states department of agriculture-agricultural research service research on natural products for pest management.recent research of the agricultural research service of usda on the use of natural products to manage pests is summarized. studies of the use of both phytochemicals and diatomaceous earth to manage insect pests are discussed. chemically characterized compounds, such as a saponin from pepper (capsicum frutescens l), benzaldehyde, chitosan and 2-deoxy-d-glucose are being studied as natural fungicides. resin glycosides for pathogen resistance in sweet potato and residues of semi-tropical leguminous ...201312846321
glycosylation of trans-resveratrol by plant-cultured cells.plant-cultured cells of catharanthus roseus converted trans-resveratrol into its 3-o-β-d-glucopyranoside, 4'-o-β-d-glucopyranoside, 3-o-(6-o-β-d-xylopyranosyl)-β-d-glucopyranoside, and 3-o-(6-o-α-l-arabinopyranosyl)-β-d-glucopyranoside. the 3-o-(6-o-β-d-xylopyranosyl)-β-d-glucopyranoside and 3-o-(6-o-α-l-arabinopyranosyl)-β-d-glucopyranoside compounds of trans-resveratrol are both new. incubation of plant-cultured cells of ipomoea batatas and strophanthus gratus with trans-resveratrol gave trans ...201222878209
carotenoid content and in vitro bioaccessibility of lutein in some leafy vegetables popular in sri lanka.lutein is one of the major carotenoids in green leafy vegetables (glvs), which show a marked antioxidant activity. the study was carried out to identify and quantify the carotenoid content of selected sri lankan glvs. rp-hplc with photodiode array detection method was followed for the separation, identification and quantification of carotenoids. among the vegetables analyzed, ipomoea batatas leaves showed the highest beta-carotene content (743.9+/-35.0 microg/g dry weight (dw)) while syngonium a ...201020651462
physicochemical properties of flours and starches derived from traditional indonesian tubers and roots.flours and starches isolated from traditional tubers and roots grown in indonesia have physical and chemical properties suitable for certain food applications. compared to other flour samples, cassava and canna flours contained the highest amount of total starch (ts) (77.4 and 77.1 %, respectively). taro starch had the lowest amount of ts among other starch samples with 75.4 %. the highest amount of amylose was observed from yam and canna flours (25.2 and 23.2 %, respectively). among starch samp ...201425477633
biogas energy production from tropical biomass wastes by anaerobic digestion.anaerobic digestion (ad) is an attractive technology in tropical regions for converting locally abundant biomass wastes into biogas which can be used to produce heat, electricity, and transportation fuels. however, investigations on ad of tropical forestry wastes, such as albizia biomass and food wastes, such as taro, papaya, and sweet potato, are limited. in this study, these tropical biomass wastes were evaluated for biogas production by liquid ad (l-ad) and/or solid-state ad (ss-ad), dependin ...201425022835
determination of acrylamide in thai-conventional snacks from nong mon market, chonburi using gc-ms technique.acrylamide in thai-conventional snacks was analysed by gc/ms with a linear response ranged of 5-50 µg and r² > 0.99. the limit of detection (s/n = 3) and limit of quantification (s/n = 10) were 4 and 15 µg kg⁻¹, respectively, and rsd < 2%. acrylamide in 19 food samples ranged from <15 µg kg⁻¹ to 1.26 mg kg⁻¹ with highest concentrations in kanom jak. moderate levels (150-500 µg kg⁻¹) were detected mostly in deep-fried products, especially sweet potato and taro crisps, kanom kai hong, banana fritt ...201224779691
heat and mass transfer in deep-frying of pumpkin, sweet potato and taro.heat and mass transfer parameters, effective thermal diffusivity, heat transfer coefficient, effective moisture diffusivity and moisture transfer coefficient-for pumpkin (cucurbita pepo), sweet potato (ipomoea batatas) and taro (colocasia esculenta) under deep-frying conditions were determined by fitting experimental data on transient values of temperature and moisture content to the solution of the standard diffusion equation in cylindrical coordinates as modified by dincer (heat mass transfer ...201023572697
effects of micronization on the physico-chemical properties of peels of three root and tuber crops.the aim of this study was to investigate the effect of micronization on the physicochemical properties of the peels of root and tuber crops, including yam (dioscorea alata l.), taro (colocasia esculenta l.) and sweet potato (ipomea batatas l.). two continuous milling sections, including hammer milling and ball milling, were applied to three samples to obtain micro-sized particles of root and tuber peels. the micronization by ball-milling treatment for 10 h was carried out to investigate the dist ...201020355109
nir determination of major constituents in tropical root and tuber crop flours.tropical root and tuber crops (cassava, sweet potato, taro, and yam) are staples in developing countries where rapid urbanization is strengthening the demand for flour based foods. quality control techniques are still under development, and when available, laboratory analyses are too expensive. the objectives of this study were to calibrate near-infrared spectroscopy (nirs) for routine analysis of flours and to test its reliability to determine their major constituents. flours prepared from 472 ...200919919112
prehistoric agricultural depletion of soil nutrients in hawai'i.we investigated the fate of soil nutrients after centuries of indigenous dryland agriculture in hawai'i using a coupled geochemical and archaeological approach. beginning approximately 500 years ago, farmers began growing dryland taro and sweet potato on the leeward slopes of east maui. their digging sticks pierced a subsurface layer of cinders, enhancing crop access to the soil water stored below the intact cinders. cultivation also catalyzed nutrient losses, directly by facilitating leaching o ...200616832047
inactivation of trypsin inhibitors in sweet potato and taro tubers during processing.in order to understand the extent of elimination of trypsin inhibitors during processing of sweet potato (ipomoea batatas) and taro (colocasia esculenta) tubers, a detailed study was conducted using tubers processed by oven drying, cooking, and microwave baking. between 80 and 90% trypsin inhibitor (ti) activity was retained in sweet potato chips up to 2h at 70 degrees c. among the four cultivars of sweet potatoes, rs-iii-2 trypsin inhibitors were more heat labile. heating at 100 degrees c led t ...200312906353
tuber storage proteins.a wide range of plants are grown for their edible tubers, but five species together account for almost 90 % of the total world production. these are potato (solanum tuberosum), cassava (manihot esculenta), sweet potato (ipomoea batatus), yams (dioscorea spp.) and taro (colocasia, cyrtosperma and xanthosoma spp.). all of these, except cassava, contain groups of storage proteins, but these differ in the biological properties and evolutionary relationships. thus, patatin from potato exhibits activi ...200312730067
alpha-amylase inhibitor changes during processing of sweet potato and taro tubers.alpha-amylase inhibitor changes during processing of sweet potatoes (ipomoea batatas) and taro (colocasia esculenta) indicated that varietal differences profoundly influence the thermal inactivation profile. the alpha-amylase inhibitors of taro were almost totally inactivated during oven drying of the chips at 90 degrees c and 100 degrees c for 24 h, while 0.8-10% activity was retained in sweet potato chips under the same conditions. relatively better thermal stability was exhibited by the sweet ...200212602936
ion chromatography of phytate in roots and tubers.the ion chromatographic method for the quantification of phytate (insp(6)) in foods was adapted for the analysis of roots and tubers. to maximize sensitivity, ultraviolet (uv) detection following postcolumn derivatization was compared with evaporative light-scattering detection (elsd). detection limits for phytate were 0.5 and 1 microg for uv and elsd, respectively. unidentified peaks eluting close to and after insp(6) were removed by solid-phase extraction. phytate was detected in 11 of 15 root ...200312517094
study on tablet binding and disintegrating properties of alternative starches prepared from taro and sweet potato tubers.to demonstrate the potential alternative sources of starch used in tablet formulations, starches from taro (ts) and sweet potato (sps) tubers were prepared with obtained yields of 11.0 and 9.6%, respectively. both ts and sps met usp22-nf17 identification and specifications. their equilibrium moisture contents and gelatinization temperatures were comparable with those of commercial starch, whereas amylose contents of ts and sps were 21.38% w/w and 41.76% w/w, respectively. both were found to poss ...200111247539
cdnas encoding for storage proteins in the tubers of taro (colocasia esculenta schott).two major protein groups of taro (colocasia esculenta) tuber were purified, and their antisera were used for the screening of the cdna library constructed from poly(a)+ rna of taro tuber. a cdna clone obtained by screening with an anti-12 kd protein antiserum had an insert 1058 bp-long, and an open reading frame for a peptide of 268 amino acids. the analyses of the n-terminal amino acid sequence and in vitro translation product suggested that the protein was synthesized as a peptide with a molec ...19938251188
distribution of nitrogen and composition of nitrogen compounds in food, urine and faeces in habitual consumers of sweet potato and taro. 19725068464
etiology of caries in papua-new guinea. associations in soil, food and water.mineral analyses of garden soils, vegetables, and water samples from 21 villages in the sepik and fly river regions of the territory of papua and new guinea, in which the mean prevalence of dental caries ranged from 0 to 29.5% decayed teeth per person, have shown the following relationships between variations in the caries prevalence and the amounts of certain elements:(1) a strong inverse association with concentrations of alkali and alkaline earth elements, especially strontium, barium, potass ...19705314012
horizontal transmission of "candidatus liberibacter solanacearum" by bactericera cockerelli (hemiptera: triozidae) on convolvulus and ipomoea (solanales: convolvulaceae)."candidatus liberibacter solanacearum" (proteobacteria) is an important pathogen of solanaceous crops (solanales: solanaceae) in north america and new zealand, and is the putative causal agent of zebra chip disease of potato. this phloem-limited pathogen is transmitted to potato and other solanaceous plants by the potato psyllid, bactericera cockerelli (hemiptera: triozidae). while some plants in the convolvulaceae (solanales) are also known hosts for b. cockerelli, previous efforts to detect li ...201526555359
rna extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment.high-quality total rna was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, norway spruce, ginkgo, japanese cedar, rose), flowers (rose, lotus japonicus) and storage tissues (seeds of lotus japonicus and rice, sweet potato tuber, banana fruit). this protocol greatly reduced the time required for rna extraction.200818603812
a cdna encoding a putative spf1-type dna-binding protein from cucumber.a cdna clone encoding a polypeptide with homology to the novel spf1 dna-binding protein of sweet potato has been isolated from a cdna library from rna of senescing cucumber (cucumis sativus, l.) cotyledons. comparison of the two sequences reveals similar features which may be important in the evolution and function of this protein, including a duplicated region of about 56 amino acids (aa). the first half of the duplicated region is enriched in basic aa and is very highly conserved, both within ...19979055825
selenium accumulating leafy vegetables are a potential source of functional foods.selenium deficiency in humans has been associated with various diseases, the risks of which can be reduced through dietary supplementation. selenium accumulating plants may provide a beneficial nutrient for avoiding such illnesses. thus, leafy vegetables such as amaranthus hybridus, amaranthus sp., cucurbita maxima, ipomoea batatas, solanum villosum, solanum scabrum, and vigna unguiculata were explored for their capabilities to accumulate selenium when grown on selenium enriched soil and for use ...201526955635
analytical optimization of a phenolic-rich herbal extract and supplementation in fermented milk containing sweet potato pulp.the aims of the present study were to optimize and characterize the phenolic composition of a herbal extract composed of green mate (ilex paraguariensis), clove (syzygium aromaticum), and lemongrass (cymbopogon citratus) and to propose the addition of this polyphenol-rich extract to fermented milks (fm) with/without sweet potato pulp (ipomoea batatas). proximate composition, ph, acidity, instrumental texture profile, total phenolic content (tpc), antioxidant activity (aa) of all formulations wer ...201727979299
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