| comparison of gamma-glutamyl hydrolase (conjugase; ec 3.4.22.12) and amylase treatment procedures in the microbiological assay for food folates. | 1. it has been suggested that deconjugation of food folates with pig kidney, compared with chicken pancreas, may increase measureable folate by approximately 50% (phillips & wright, 1983). therefore deconjugation with conjugases from these two enzyme sources was optimized and compared. folate was measured microbiologically with lactobacillus casei (atcc 7469) as the test organism at ph 5.6. 2. treatment for 6 h with 200 mg pig kidney/200 mg sample was compared with the conventional assay employi ... | 1988 | 3129012 |
| surface carbohydrate changes on onchocerca lienalis larvae as they develop from microfilariae to the infective third-stage in simulium ornatum. | use was made of seven fitc labelled lectins as tools to investigate the surface of onchocerca lienalis larvae as they develop through to the infective third-stage in a natural vector, simulium ornatum. the lectins were derived from canavalia ensiformis (con a), lens culinaris (lentil), triticum vulgaris (wheat germ), arachis hypogaea (peanut), helix pomatia, phaseolus vulgaris (kidney bean) and tetragonolobus purpureus (asparagus pea). between 70 and 100 living parasites were examined for each d ... | 1988 | 3142948 |
| parameters involved in binding of porcine pancreatic alpha-amylase with black bean inhibitor: role of sulfhydryl groups, chloride, calcium, solvent composition and temperature. | the amylase inhibitor of black (kidney) beans (phaseolus vulgaris; mw 53,000) forms a 1:1 stoichiometric complex with porcine pancreatic alpha-amylase (mw 52,000) at ph 5.40. the single sulfhydryl group of the inhibitor and the two sulfhydryl groups of alpha-amylase are not involved in recognition and binding. chloride ions, required for activity of alpha-amylase at both ph 5.40 and 6.90, are important for inhibitor--enzyme binding at ph 6.90 but not at ph 5.40. calcium-free alpha-amylase binds ... | 1988 | 3147707 |
| [characterization of the nutritional potential of tepari bean (phaseolus acutifolius) grown in mexico]. | the chemical, physical, antiphysiological and biological quality of the protein of three varieties (white, brown and black) of tepary bean (phaseolus acutifolius) were studied. its proximate composition was determined, as well as water absorption, hardness of the seed, tannin content, trypsin inhibitors and lectins. beans were also submitted to differential scanning calorimetry of the starch. the biological quality of its proteins was also established, based on the protein efficiency ratio (per) ... | 1988 | 3154299 |
| axonal projections from bötzinger expiratory neurons to contralateral ventral and dorsal respiratory groups in the cat. | we studied projection patterns of the augmenting expiratory neurons of the bötzinger complex (bot) in the contralateral brainstem. three experimental approaches were used: 1) electrophysiological analysis using antidromic microstimulation, and morphological analyses using 2) intraaxonal injection of hrp, and 3) application of the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l). taken together, the three methods revealed morphological details of the axonal arborizations of the expir ... | 1988 | 3169184 |
| anterograde neuroanatomical tracing with phaseolus vulgaris-leucoagglutinin combined with immunocytochemistry of gamma-amino butyric acid, choline acetyltransferase or serotonin. | in order to associate specific fiber projections in the central nervous system with specific target neurons, procedures were developed in which the anterograde neuroanatomical tracing technique utilizing phaseolus vulgaris-leucoagglutinin (pha-l) is combined with immunocytochemistry of three (different) neuronal markers: gamma-amino butyric acid, choline acetyltransferase, and serotonin. a double, indirect, peroxidase-antiperoxidase staining method is used on free-floating brain sections. the pr ... | 1988 | 3170265 |
| further observations on the relationship between adenosine deaminase-containing axons and trigeminal mesencephalic neurons: an electron microscopic, immunohistochemical and anterograde tracing study. | the somas of primary afferent neurons in the mesencephalic nucleus of the trigeminal nerve in rat have a dense investment of axons immunoreactive for the enzyme adenosine deaminase. we previously suggested that these axons may originate from adenosine deaminase-immunoreactive neurons located in the tuberomammillary nucleus of the hypothalamus [nagy et al. (1986) neuroscience 17, 141-156]. anterograde tracing and immunohistochemical techniques were used to investigate this possibility further. in ... | 1988 | 3173693 |
| muscle protein synthesis of rats fed a kidney bean (phaseolus vulgaris l.) diet. | | 1988 | 3175251 |
| oral feeding of isolated lectins from red kidney bean stimulates rat small intestinal mucosal dna synthesis and crypt cell division. | a lectin preparation containing enterokinase inhibitor purified or partially purified from red kidney bean (rkb) when fed to weanling rats was shown to cause small intestinal hyperplasia. to see if this hyperplastic effect on the rat small intestine was due to the mitogenic properties of the isolated lectin, male weanling rats were fed a chow containing 0.1% of the isolated lectin for six days. age-matched control rats were fed regular chow. both control and lectin-fed rats were sacrificed at on ... | 1988 | 3180972 |
| glycoconjugate distribution in normal human endometrium. a lectin histochemical study. | a battery of nine lectins was applied to routinely processed endometrial biopsies to evaluate changes in glycoconjugate expression during the menstrual cycle and early gestation. specific staining patterns emerged for glands, surface epithelium, predecidual and decidual cells, stromal granulocytes, and endothelium. three lectins--wheat germ agglutinin (wga), ricinus communis-i (rca-i), and concanavalin ensiformis (con-a)--showed consistent binding to epithelial and stromal cells in the menstrual ... | 1988 | 3182170 |
| changes in septo-hippocampal projections after lateral entorhinal or combined entorhinal-raphé lesions as studied by anterograde tracing methods. | septal and entorhinal projections to the hippocampus show a considerable overlap in their target structures in the molecular layer of the dentate gyrus (dg) and stratum lacunosum-moleculare of the cornu ammonis (ca). employing anterograde tracing methods, it was investigated in which way the morphological pattern of the septohippocampal projections were influenced by lateral entorhinal cortex (lea) lesions. anterograde filling of neurons from soma to axonal terminals with phaseolus vulgaris leuc ... | 1988 | 3191413 |
| evidence for direct projections from the nucleus of the solitary tract onto medullary adrenaline cells. | the anatomic relationships between projections from the intermediate portion of the nucleus of the solitary tract (nts) and phenylethanolamine n-methyl transferaselike-immunoreactive (pnmt-li) cells in the medulla oblongata were analyzed in sprague-dawley rats by using two-color immunoperoxidase staining combined with the anterograde transport of phaseolus vulgaris leucoagglutinin (pha-l). pha-l was iontophoretically deposited in the intermediate region of the nts and the subjacent dorsal motor ... | 1988 | 3192770 |
| climbing fibers are labelled after injection of pha-l into the nucleus interpositus of the cat. | after injection of phaseolus vulgaris-leucoagglutinin (pha-l) into the interposed nucleus of the cat, labelled fibers were found in the granular layer as well as in the molecular layer of the cerebellar cortex. those in the molecular layer were judged from their location and morphology as climbing fibers. such labelling of climbing fibers which have not been labelled by autoradiography could be attributed to higher sensitivity and resolution of pha-l method. in addition, purkinje cell soma and i ... | 1988 | 3196903 |
| an ultrastructural study of the morphology and lectin-binding properties of human mast cell granules. | the morphological characteristics and lectin-binding properties of mast cell granules from four human neurofibromata are described. ultrastructural examination of the granules revealed that some contained dense cores, others had membranous configurations and some forms were intermediate between the two. a round electron-lucent area was present in some granules. after treatment with biotinylated lectins (10 micrograms ml-1) followed by an avidin-peroxidase revealing system (5 micrograms ml-1 in 0 ... | 1988 | 3198420 |
| parabrachial innervation of the cat's dorsal lateral geniculate nucleus: an electron microscopic study using the tracer phaseolus vulgaris leucoagglutinin (pha-l). | ascending pathways from the brain stem play a key role, generally facilitatory, in controlling the transmission of retinal information through the lateral geniculate nucleus to the visual cortex (for reviews, see singer, 1977; burke and cole, 1978; sherman and koch, 1986). in order to characterize the morphological basis of this brain-stem control, we used the electron microscope to study synaptic terminals labeled anterogradely from injections of the tracer phaseolus vulgaris leucoagglutinin in ... | 1988 | 3199193 |
| the connections between the septum-diagonal band complex and histaminergic neurons in the posterior hypothalamus of the rat. anterograde tracing with phaseolus vulgaris-leucoagglutinin combined with immunocytochemistry of histidine decarboxylase. | the connections between nuclei of the septum-diagonal band complex and the clusters of histaminergic neurons in the posterior hypothalamic region were studied with a dual-labeling procedure in which anterograde neuroanatomical tracing with phaseolus vulgaris-leucoagglutinin was combined with immunohistochemistry of histidine decarboxylase. phaseolus vulgaris-leucoagglutinin was injected in the medial and lateral septal nuclei, and in various parts of the nuclei of the diagonal band of broca. the ... | 1988 | 3200431 |
| purification, characterization and induction of l-phenylalanine ammonia-lyase in phaseolus vulgaris. | the enzyme l-phenylalanine ammonia-lyase was purified from leaves of phaseolus vulgaris by sephacryl s-200 gel filtration and sepharose-4-b--succinyl-aminoethyl-l-phenylalanine affinity chromatography. l-phenylalanine ammonia-lyase was specifically eluted from the affinity matrix with its substrate l-phenylalanine at 20-25 degrees c. the purified enzyme was shown to be homogeneous by gel electrophoresis both in presence and absence of sds. its mr, determined by gel filtration and non-denaturing ... | 1988 | 3203691 |
| basal forebrain inputs to the interpeduncular nucleus in the rat studied with the phaseolus vulgaris-leucoagglutinin tracing method. | the connections between the basal forebrain and the interpeduncular nucleus (ip) were studied in the rat using the anterograde transport of phaseolus vulgaris-leucoagglutinin (pha-l). pha-l was injected in the septum-diagonal band complex, the preoptic area, the substantia innominata, the globus pallidus, and the ventral pallidum. in a number of cases sections of the ip were double-immunostained for pha-l and serotonin. only following pha-l injections in the medial septal nucleus and the nucleus ... | 1988 | 3208152 |
| projections of submucosal neurons to the myenteric plexus of the guinea pig intestine: in vitro tracing of microcircuits by retrograde and anterograde transport. | the enteric nervous system (ens) can mediate reflex activity without input from the brain or spinal cord. the ens thus contains intrinsic primary afferent neurons that link mucosal sensory receptors with motor neurons in the myenteric plexus. the intrinsic primary afferent neurons of the gut have not yet been identified. although the submucosal plexus is known to innervate the mucosa, where enteric sensory receptors are located, no submucosal to myenteric projections have previously been found. ... | 1988 | 3209743 |
| organization of feedback and feedforward projections of the barrel cortex: a pha-l study in the mouse. | in order to analyze the organization of the efferent projections of single barrel columns (bc, i.e. a barrel in layer iv of parietal cortex plus the cortical tissue above and below it), we made small iontophoretic injections of the anterograde tracer phaseolus vulgaris leucoagglutinin in the barrel cortex of 20 adult mice. on the basis of reconstructions of the sites of terminal labelling, the brain regions receiving projections from the barrel cortex could be identified and classified in five g ... | 1988 | 3215316 |
| duodenal toxicity of dietary phaseolus vulgaris lectins in the rat: an integrative assay. | it is now generally admitted that phytohemagglutinin (pha) constitutes the main factor responsible for the dietary toxicity of raw kidney beans. in the growing rat, an impairment of growth is the unique expression of a malnutrition syndrome. the aim of this work was to precise to what extent the intestinal injuries may account for this malnutrition. pha was administered for 9 days to growing rats at levels ranging from 0.0025 to 0.25% of food dry matter. one group of controls was fed ad libitum ... | 1988 | 3220180 |
| neural connections identified with pha-l anterograde and hrp retrograde tract-tracing techniques. | this paper describes a method of identifying specific input and output elements of a two-neuron projection pathway. phaseolus vulgaris leucoagglutinin (pha-l) anterograde tract-tracing was used in combination with the retrograde transport of horseradish peroxidase (hrp) to demonstrate connections between small groups of neurons in the brainstem auditory system. specifically, the projection from cochlear nucleus to olivary neurons that project to the cochlea were demonstrated. the first neuron in ... | 1988 | 3221683 |
| direct projection of the corticospinal tract to the superficial laminae of the spinal cord in the rat. | the anterograde transport of both wheat germ agglutinin conjugated to horseradish peroxidase and the kidney bean lectin phaseolus vulgaris leucoagglutinin was utilized to investigate the projection of primary sensorimotor corticospinal tract axons to the superficial laminae of the spinal dorsal horn in the rat. both methods yielded qualitatively similar patterns of connectivity. corticospinal tract axons were found to terminate within all laminae on the side contralateral to the injection site. ... | 1988 | 3230165 |
| mycotoxin-producing potential of fungi isolated from red kidney beans. | the predominant fungi present in samples of reject and retail red kidney beans were aspergillus glaucus, penicillium spp. and alternaria spp. together with a. ochraceus, a. flavus, fusarium spp., and trichoderma, these isolates from the reject beans were screened for numerous mycotoxins by tlc. the most consistently produced mycotoxins were penicillic acid (from a. ochraceus and penicillium spp.) and alternaria toxins (tenuazonic acid and alternariol). a. glaucus strains were tested for cytotoxi ... | 1988 | 3237243 |
| plasticity of intra-amygdaloid connections following the denervation of fibers from accessory olfactory bulb to medial amygdaloid nucleus in adult rat: immunohistochemical study of anterogradely transported lectin (phaseolus vulgaris leucoagglutinin). | the rearrangement of the terminations of intra-amygdaloid fibers was examined in the medial amygdaloid nucleus (man) following denervation of the fibers from the accessory olfactory bulb (aob) of adult rat using immunohistochemistry of anterogradely transported lectin (phaseolus vulgaris leucoagglutinin, pha-l). the aob was removed unilaterally by suction at two months before the pha-l injection. the pha-l was injected bilaterally into the posteromedial region of the amygdala (posteromedial amyg ... | 1988 | 3251586 |
| cholinergic projections from the laterodorsal and pedunculopontine tegmental nuclei to the pontine gigantocellular tegmental field in the cat. | this study demonstrates that the laterodorsal tegmental nucleus (ldt) and pedunculopontine tegmental nucleus (ppt) are sources of cholinergic projections to the cat pontine reticular formation gigantocellular tegmental field (pftg). neurons of the ldt and ppt were double-labeled utilizing choline acetyltransferase immunohistochemistry combined with retrograde transport of horseradish peroxidase conjugated with wheat germ agglutinin (wga-hrp). in the ldt the percentage of cholinergic neurons retr ... | 1988 | 3251602 |
| selective inhibition of histidine-modified pancreatic alpha-amylase by proteinaceous inhibitor from phaseolus vulgaris. | chemical modification of two histidine residues of porcine pancreatic alpha-amylase (ec 3.2.1.1) by diethyl pyrocarbonate in the presence of a high concentration of maltotriose caused a decrease of amylase activity and an increase of maltosidase activity (hydrolysis of p-nitrophenyl-alpha-maltoside). by binding a proteinaceous inhibitor from phaseolus vulgaris (white kidney bean) with the modified enzyme, the amylase activity was further decreased but the maltosidase activity was retained to abo ... | 1988 | 3259857 |
| projections of the medial preoptic nucleus: a phaseolus vulgaris leucoagglutinin anterograde tract-tracing study in the rat. | the projections of the medial preoptic nucleus (mpn) were examined by making injections of the anterogradely transported lectin phaseolus vulgaris leucoagglutinin (pha-l) into the mpn and charting the distribution of labeled fibers. the evidence indicates that the mpn projects extensively to widely distributed regions in both the forebrain and brainstem, most of which also supply inputs to the nucleus. an important neuroendocrine role for the mpn is underscored by its extensive projections to al ... | 1988 | 3259955 |
| monoclonal antibodies against leucoagglutinin-reactive human t-lymphocyte surface components. i. characterization of cellular binding sites. | the binding specificities of three biologically active anti-lymphocyte monoclonal antibodies (moab) (k46m, k3g, and 3-19-2) produced against human t-cell surface components reactive with the mitogenic lectin leucoagglutinin from phaseolus vulgaris (la) were analysed. k46m is a strong t-cell mitogen, while k3g and 3-19-2 inhibited cell-mediated cytotoxicity. resting peripheral blood lymphocytes (pbl) contained 4-16% k46m+ cells, 8-35% k3g+ cells, and less than 0.3-4% 3-19-2+ cells. after stimulat ... | 1988 | 3266026 |
| compromises between penetration of antisera and preservation of ultra structure in pre-embedding electron microscopic immunocytochemistry. | light microscopic pre-embedding immunocytochemistry on free-floating brain sections is commonly performed with antisera to which a detergent is added. for instance, the trajectories and the terminals of axons of neurons labelled by anterograde neuroanatomical tracing with phaseolus vulgaris-leucoagglutinin (pha-l) are commonly visualized by immunocytochemistry, using antisera to which 0.5 per cent triton x-100 is added. in this material the ultrastructural details are very poorly preserved. omit ... | 1988 | 3267342 |
| efferent projections of the suprachiasmatic nucleus: i. studies using anterograde transport of phaseolus vulgaris leucoagglutinin in the rat. | the efferent projections of the suprachiasmatic nucleus (sch) in the rat hypothalamus have been reexamined with the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l), which displays labeled axons with the clarity of a golgi impregnation. fibers from the sch can be divided into six pathways for descriptive purposes. by far the densest terminal field arising from cells in the sch ends in a roughly comma-shaped zone between the sch and paraventricular nucleus on the one hand and the per ... | 1987 | 3294923 |
| vasopressin cells in the medial amygdala of the rat project to the lateral septum and ventral hippocampus. | the rat brain contains a large number of vasopressin (vp) immunoreactive fibers, the sites of origin of which have not yet been established completely. for instance, the sources of vp fiber systems in the amygdala, ventral hippocampus (vh), mediodorsal thalamic nucleus, ventral tegmental area, and dorsal raphe yet remain obscure. these vp fibers may originate in any of the recently described extrahypothalamic vp cell groups, viz., medial amygdaloid nucleus (ame), dorsomedial hypothalamic nucleus ... | 1987 | 3305600 |
| transplant-derived astrocytes migrate into host lumbar and cervical spinal cord after implantation of e14 fetal cerebral cortex into adult thoracic spinal cord. | fourteen-day gestation fetal cerebral cortex homografts were transplanted into the thoracic (t6) spinal cord between the left dorsal column and dorsal horn of adult host rats. the transplants were soaked in 2.0 micrograms/ml of the lectin phaseolus vulgaris leucoagglutinin (phal) prior to implantation. transplanted host spinal cords were utilized at 7, 14, and 24 d and at 1 and 2 months postimplantation. paraffin-sectioned spinal cords were double labeled for phal and glial fibrillary acidic pro ... | 1987 | 3305970 |
| rapid induction by fungal elicitor of the synthesis of cinnamyl-alcohol dehydrogenase, a specific enzyme of lignin synthesis. | a fivefold increase in the extractable activity of cinnamyl-alcohol dehydrogenase, an enzyme of phenylpropanoid metabolism specific for lignin synthesis, was observed within 10 h of treatment of cell-suspension cultures of bean (phaseolus vulgaris l.) with a high-molecular-mass elicitor preparation heat-released from mycelial cell walls of the bean pathogen colletotrichum lindemuthianum. elicitor caused a rapid, marked but transient increase in the synthesis of cinnamyl-alcohol dehydrogenase wit ... | 1987 | 3315665 |
| the plant vacuolar protein, phytohemagglutinin, is transported to the vacuole of transgenic yeast. | phytohemagglutinin (pha), the major seed lectin of the common bean, phaseolus vulgaris, accumulates in the parenchyma cells of the cotyledons. it has been previously shown that pha is cotranslationally inserted into the endoplasmic reticulum with cleavage of the nh2-terminal signal peptide. two n-linked oligosaccharide side chains are added, one of which is modified to a complex type in the golgi apparatus. pha is then deposited in membrane-bound protein storage vacuoles which are biochemically ... | 1987 | 3316244 |
| migration of cultured fetal spinal cord astrocytes into adult host cervical cord and medulla following transplantation into thoracic spinal cord. | cell suspensions from 14-day-gestation rat spinal cord, which had previously been soaked for 1 hr in a 2 micrograms/ml solution of phaseolus vulgaris leucoagglutinin (phal), were cultured on collagen gels containing laminin for 2 weeks. pieces of the gel and attached cells were then transplanted into the dorsal column of adult host thoracic spinal cord. at 1, 2, and 3 months postimplantation (mpi), animals were sacrificed, and the spinal cords were removed, embedded in paraffin, and sectioned at ... | 1988 | 3343709 |
| comparative study on the main membrane-surface sialoglycopeptides released from young and old human erythrocytes with trypsin. | 1. the amount of sialoglycopeptide (sgp) mixture released from young (y) erythrocytes by trypsin treatment was significantly larger than that from old (o) ones. 2. the two main membrane-surface sialoglycopeptides (y-sgp and o-sgp) were isolated in yields of about 45% and 42% from the mixtures by a combination of sephadex g-50 superfine gel filtration with deae-cellulose chromatography, respectively. molecular weights of 12,000 and 12,100, respectively, were found for the y-sgp and the o-sgp. 3. ... | 1988 | 3356136 |
| neuronal structures of the brainstem participating in postural suppression in cats. | two histochemical tracer techniques (a horseradish peroxidase and a phaseolus vulgaris leucoagglutinin axonal flow technique) were employed to determine the cells of origin, axonal trajectories and terminals of fibers of passage through the dorsal tegmental field (dtf) in the pons along the midline (horsley-clarke coordinates, p 3 to p 7, lr 0, h - 4.5 to h - 6.0). stimulation of the dtf area results in the postural suppression in both acute decerebrate, reflexively standing cats and freely movi ... | 1988 | 3357584 |
| selective effects of pha on rat brush border hydrolases along the crypt-villus axis. | the mechanism of the toxicity of lectin from phaseolus vulgaris seeds has been investigated on rat enterocytes. cell isolation procedures showed a selectivity in the loss of brush border hydrolases; this indicated that the microvilli blebbing was not the only mechanism of action of lectins on rat enterocytes. | 1988 | 3360085 |
| monosynaptic transmission of respiratory drive to phrenic motoneurons from brainstem bulbospinal neurons in rats. | the termination patterns in the rat phrenic nucleus of neurons within two respiratory cell groups of the ventrolateral medulla (bötzinger complex and the rostral ventral respiratory group) were determined. the plant lectin, phaseolus vulgaris leuco-agglutinin, was used as an anterograde tracer to label presynaptic processes of bulbospinal neurons, and horseradish peroxidase was used simultaneously to label phrenic motoneurons. labeled bulbospinal axons ended with dense terminal arborizations wit ... | 1988 | 3361003 |
| [the effect of exogenous factors on pre-cecal nutrient and amino acid resorption determined in swine with ileo-rectal anastomoses. 2. the effect of the degree of grinding of plant protein-rich concentrates in rations]. | in digestion trials using pigs fitted with ileo-rectal anastomoses and intact pigs, the influence of two different grinding finenesses (coarse, fine) of vegetable high-protein concentrates (soya bean meal, field bean, lupin) added to cereal diets was examined. it was found that neither the precaecal nor the total nutrient absorption and digestibility were practically injured, when in the concentrates the particle sizes bigger than 2 mm did not amount more than 20% and whole grains hardly occurre ... | 1988 | 3369941 |
| entorhinal projections to the hippocampal ca1 region in the rat: an underestimated pathway. | the projections of the entorhinal cortex to ca1 in relation to the entorhinal-dentate projections were studied in the rat, using the anterograde transport of phaseolus vulgaris leucoagglutinin. it was observed that the entorhinal cortex is heterogeneous with respect to the origin of these projections. caudomedial portions of the entorhinal cortex mainly distribute fibers to the fascia dentata, whereas only a minor projection reaches ca1. progressively more rostral and lateral parts of the entorh ... | 1988 | 3374835 |
| intrinsic connections of the retrohippocampal region in the rat brain: iii. the lateral entorhinal area. | this paper describes the retrohippocampal projections of individual layers of the lateral entorhinal area as studied by the method of anterograde transport of the lectin phaseolus vulgaris leucoagglutinin (pha-l) in the rat. as in the medial entorhinal area (ea), (köhler, '86a) pha-l injections restricted to individual layers of the lateral ea resulted in labeling of sparse projections to the subicular complex (e.g., subiculum, pre- and parasubiculum), whereas projections to the perirhinal area ... | 1988 | 3379162 |
| description of a large projection from the mesodiencephalic junction to the rostral red nucleus. an anatomical study in the rat and in the cat. | horseradish peroxidase (hrp) or wheat germ agglutinin conjugated to hrp (wga-hrp) were deposited in the rostral pole (rpc) of the red nucleus in 6 rats and 5 cats. in the rat, a zone of dense retrograde cell labeling and of diffuse axonal labeling occurred in the sub-pretectal area, a region which corresponds to the posterior thalamic nucleus (pt) of bold et al. (brain research, 12 (1984) 521-527). in the cat, a similar retrograde and anterograde pattern of labeling was observed. the labeled are ... | 1988 | 3382523 |
| efferent projections of inspiratory neurons of the ventral respiratory group. a dual labeling study in the rat. | the efferent projections of the medullary respiratory neurons of the rat were studied using an anterograde tracer, phaseolus vulgaris leucoagglutinin (pha-l). in nembutal-anesthetized rats, pha-l was iontophoretically applied to (1) the area of inspiratory neurons of the ventral respiratory group (vrg) around the nucleus ambiguus, or (2) the area ventrolateral to the solitary tract. in addition, a fluorescence retrograde tracer, fast blue (fb), was injected into the cervical phrenic nerve severa ... | 1988 | 3401784 |
| glycosylation of three molecular forms of human alpha 1-acid glycoprotein having different interactions with concanavalin a. variations in the occurrence of di-, tri-, and tetraantennary glycans and the degree of sialylation. | human alpha 1-acid glycoprotein (agp) was separated into a non-bound (agp-a; 46%), a retarded (agp-b; 39%) and a bound fraction (agp-c; 15%) using concanavalin a (cona)-sepharose chromatography. the apparent molecular masses, as determined by sds-page, of the three fractions were 43.5, 42.3 and 41.2 kda, respectively. the occurrence of n-linked di-, tri- and tetraantennary glycans on these three molecular forms (agp-a, -b, and -c) was studied by sequential lectin-affinity chromatography of the 1 ... | 1988 | 3402460 |
| reproductive performance of hens fed field beans and potential relationships to vicine metabolism. | field beans (faba beans, broad beans, horse beans, ackerbohnen, vicia faba l.) depress weight of eggs when fed to laying hens. vicine isolated from field beans has been shown to depress egg weight and hatchability when fed in large amounts. the present study was undertaken to determine the effect of using three varieties of field beans, varying in tannin and total vicines content, at 20% in breeder diets on reproductive performance of hens and associated vicine transfer from the diet to the egg. ... | 1988 | 3405925 |
| afferent projections to the mesencephalic trigeminal nucleus in the rat. anterograde tracing with phaseolus vulgaris leukoagglutinin. | the anatomical pathways from the parvocellular reticular formation and medulla oblongata to the mesencephalic trigeminal nucleus (mes v) were investigated in the rat by means of the anterograde tracer phaseolus vulgaris leukoagglutinin. p. vulgaris was injected into the rostral ventromedial medulla, rostral and caudal ventrolateral medulla, the parvocellular reticular formation and surrounding regions. rostral medullary regions and the parvocellular reticular formation projected to mes v althoug ... | 1988 | 3414374 |
| fetal cortical astrocytes migrate from cortical homografts throughout the host brain and over the glia limitans. | the cerebral cortices from 14-day gestation rat embryos were prelabeled with phaseolus vulgaris leucoagglutin (phal) and then homografted into freshly made implantation pockets in the host cerebral cortex. animals were sacrificed at 30 and 60 days postimplantation (dpi). paraffin sections were double labeled for the presence of glial fibrillary acidic protein (gfap), a specific marker for astrocytes, and phal, utilized as a marker for transplant-derived cells. transplant-derived astrocytes were ... | 1988 | 3418752 |
| liver and muscle proteolytic activity in field bean (vicia faba l.) fed birds. effect of vitamin e. | liver and muscle proteolytic activities (cathepsin a and d) were measured in growing male chickens fed ad libitum over periods of 30 and 60 days on 20% protein diets containing either heated soybean (hsb, control) or raw field bean (rfb, vicia faba l.) as the main sources of protein. vitamin e (250 mg/100 g diet) was added or not to the rfb diet. it has been found that in comparison to control hsb-fed animals, rfb-fed birds showed a significant reduction in the rate of growth, together with a si ... | 1987 | 3423398 |
| [preparation of soluble extracts with mitogenic activity on human lymphocytes from 3 varieties of common beans (phaseolus vulgaris). determination of their reliability for clinical use]. | | 1987 | 3426353 |
| distribution of locus coeruleus axons in the rat spinal cord: a combined anterograde transport and immunohistochemical study. | the distribution of locus coeruleus axons in the rat spinal cord was studied by anterograde transport of phaseolus vulgaris leucoagglutinin in combination with dopamine-beta-hydroxylase immunohistochemistry. locus coeruleus axons were observed primarily in the superficial laminae of the dorsal horn. few locus coeruleus fibers were seen in the vicinity of motoneuron pools or in the intermediolateral cell column of the thoracic spinal cord. the results of this study suggest a selective action of t ... | 1987 | 3427477 |
| [role of polyphenols in the low nutritive value of vicia faba]. | the inclusion of field bean (vicia faba) in diets for growing experimental animals, reduced their growth performance in a similar way as compared with controls fed a casein diet, with 0.2% tannic acid. hydroalcoholic extracts or isolated tannins obtained from vicia faba seeds, decreased the intestinal transport of d-glucose. the addition of 0.05, 0.1 or 0.2% of tannic acid to the perfusion solutions containing d-glucose proportionally diminished the intestinal uptake of sugar according to its po ... | 1987 | 3455186 |
| nucleotide sequence of a bean (phaseolus vulgaris) u1 small nuclear rna gene: implications for plant pre-mrna splicing. | we have isolated a u1 small nuclear rna (snrna) gene from common bean (phaseolus vulgaris). the haploid bean genome contains only one or a few copies of the u1 snrna gene. the bean and human u1 snrna genes are 65% homologous but share no significant similarity in the 5' or 3' flanking regions. the predicted secondary structure of bean u1 snrna is identical to that of other u1 snrnas, and the sequences of the single-stranded regions have been highly conserved. thus, both the sequence of the singl ... | 1987 | 3480532 |
| chemical and biological characterization of low-molecular-weight human skeletal growth factor. | skeletal growth factor (sgf) activity was extracted from human bone matrix by demineralization and purified under dissociative conditions using hydroxyapatite, hplc gel-filtration and hplc reverse-phase chromatography. human sgf thus purified was characterized chemically and biologically. purified human sgf stimulated chick embryo bone cell proliferation at picomolar concentrations (half maximum at 2-3 ng/ml) and had little or no activity on other cell types tested (mouse 3t3 and normal rat kidn ... | 1986 | 3490278 |
| an alpha-amylase inhibitor from cranberry bean (phaseolus vulgaris): its specificity in inhibition of mammalian pancreatic alpha-amylases and formation of a complex with the porcine enzyme. | a proteinaceous inhibitor that inhibits mammalian alpha-amylases was prepared from cranberry bean and examined for its reactivity with alpha-amylases from various origins. the cranberry bean alpha-amylase inhibitor (cbai) exhibited inhibitory effects on pancreatic alpha-amylases from the following mammals: pig, dog, cat, horse, sheep, cow, rabbit, guinea pig, rat, and mouse. cbai showed a maximal inhibition at ph 5.5 against porcine pancreatic alpha-amylase (ppa). it was confirmed by gel filtrat ... | 1987 | 3502137 |
| lectin binding to distinguish cell types in fixed atherosclerotic arteries. | in order to assess the possible utility of lectin binding to identify the cellular components of fixed arterial lesions we studied lectin binding in experimental rabbit and monkey vessels, as well as in human atherosclerotic arteries obtained at surgery. the avidin-biotin-peroxidase technique was used to localize the binding of the following biotinylated lectins: concanavalin a (con a), dolicho biflorus agglutinin (dba), soybean agglutinin (sba), peanut agglutinin (pna), phaseolus vulgaris agglu ... | 1986 | 3533093 |
| immunogold localization of ingested kidney bean (phaseolus vulgaris) lectins in epithelial cells of the rat small intestine. | the interactions between dietary kidney bean (phaseolus vulgaris) lectins and the epithelial cells of the rat small intestine were investigated by immunogold electron microscopy. the results demonstrated that the lectins bind to the glycocalyx of duodenal and jejunal microvilli and that some of these dietary constituents are endocytosed into lysosomal pathways within both absorptive and secretory gut cells. it is concluded that the lysosomal response serves to limit the absorption of nutritional ... | 1986 | 3536802 |
| in vitro synthesis of bean (phaseolus vulgaris) chloroplastic and cytoplasmic leucyl-trna synthetases. characterization and processing of a precursor polypeptide for the chloroplast enzyme. | bean (phaseolus vulgaris) chloroplastic and cytoplasmic leucyl-trna synthetases differ in their structural and catalytic properties and do not share common antigenic determinants. polyadenylated mrnas, prepared from young bean leaves, have been translated in vitro in a rabbit reticulocyte lysate cell-free system. the newly synthesized polypeptides have been submitted to immunoadsorption on protein a-sepharose in the presence of the antibodies raised against the chloroplastic or the cytoplasmic l ... | 1987 | 3549728 |
| projections of nucleus paragigantocellularis lateralis to locus coeruleus and other structures in rat. | the projections of the retrofacial portion of nucleus paragigantocellularis lateralis (retrofacial pgcl) were mapped in the rat with the phaseolus vulgaris leucoagglutinin anterograde tracing technique. this structure projects to a restricted number of bulbar or spinal nuclei involved in autonomic regulation, principally the intermediolateral cell column of the spinal cord, the nucleus tractus solitarius complex, the lateral parabrachial and kolliker-fuse nuclei and the ventrolateral medulla. re ... | 1987 | 3552117 |
| liver and muscle proteolytic activity in male growing rats fed a field bean (vicia faba l.) diet. | total liver cathepsin a and d and gastrocnemius acid proteolytic activities have been evaluated in male growing rats fed ad libitum over periods of 15 and 30 days on 20%-protein diets containing either casein or raw field bean (vicia faba l.) as protein sources. it has been found that, compared to the control casein-fed rats, those fed the legume diet exhibited a marked reduction (p less than 0.05) in the rate of growth and protein efficiency rate; liver proteolytic activity increased with agein ... | 1987 | 3556160 |
| anatomical and electrophysiological evidence for a projection from the medial preoptic area to the 'mesencephalic and subthalamic locomotor regions' in the rat. | there is considerable physiological evidence indicating that the medial preoptic area plays an important role in neural circuits mediating ingestive, thermoregulatory, and reproductive behaviors, all of which involve foraging. the current series of anatomical and electrophysiological experiments was therefore designed to characterize a direct projection from the medial preoptic area to a region in the zona incerta just dorsal to the subthalamic nucleus, which appears to lie within the 'subthalam ... | 1987 | 3567588 |
| evaluation of structural and secretory glycoconjugates in normal human jejunum by means of lectin histochemistry. | the labelling pattern of eight lectins was studied in jejunal samples from ten normal subjects, in order to define the normal distribution of structural and secretory glycoconjugates in the small bowel. the following lectins were studied by means of a peroxidase technique on formalin-fixed samples: arachis hypogaea, ricinus communis, canavalia ensiformis, lens culinaris, phaseolus vulgaris, triticum vulgaris, ulex europaeus, dolichos biflorus. phaseolus vulgaris reacted with goblet cell mucus th ... | 1987 | 3570880 |
| an assay for leukoagglutinating lectins using suspension cultured mouse lymphoma cells (bw5147) stained with neutral red. | a sensitive and rapid assay for leukoagglutinating lectins has been developed. this assay utilizes neutral red-stained mouse lymphoma cells from the suspension cultured cell line bw5147. the agglutination of the stained cells can be monitored visually in a manner similar to that for conventional assays for erythroagglutinating lectins using erythrocytes. the activity of lekoagglutinating lectins that are not capable of agglutinating erythrocytes can be quantified by this assay. the utility of th ... | 1987 | 3578790 |
| nitrogen-fixing nodules induced by agrobacterium tumefaciens harboring rhizobium phaseoli plasmids. | rhizobium phaseoli cfn299 forms nitrogen-fixing nodules in phaseolus vulgaris (bean) and in leucaena esculenta. it has three plasmids of 185, 225, and 410 kilobases. the 410-kilobase plasmid contains the nitrogenase structural genes. we have transferred these plasmids to the plasmid-free strain agrobacterium tumefaciens gmi9023. transconjugants containing different combinations of the r. phaseoli plasmids were obtained, and they were exhaustively purified before nodulation was assayed. only tran ... | 1987 | 3584072 |
| [comparative evaluation of the hypoglycemic activity of the vegetal complex of phaseolus vulgaris and chlorpropamide in experimental diabetes]. | experiments on rabbits with alloxan diabetes showed that the plant complex (pc) reduced the level of glycemia after single administration for 6-8 h by 27-32%. a similar effect was demonstrated with chlorpropamide. however the pc produced a longer hypoglycemic effect. in course treatment the pc returned the blood level of glucose (5.14 +/- 0.62 mmol/l) to normal on the 11th day whereas with chlorpropamide this indicator was almost normal (6.6 +/- 1.1 mmol/l) on the 15th day only. a rapid decrease ... | 1987 | 3601894 |
| cortical projection patterns of magnocellular basal nucleus subdivisions as revealed by anterogradely transported phaseolus vulgaris leucoagglutinin. | the present paper deals with a detailed analysis of cortical projections from the magnocellular basal nucleus (mbn) and horizontal limb of the diagonal band of broca (hdb) in the rat. the mbn and hdb were injected iontophoretically with the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l). after immunocytochemical visualization of labeled efferents, the distribution of projections over the cortical mantle, olfactory regions and amygdala were studied by light microscopy. based on dif ... | 1987 | 3300852 |
| identification of synaptic terminals of thalamic or cortical origin in contact with distinct medium-size spiny neurons in the rat neostriatum. | in order to determine what types of neurons in the striatum receive direct synaptic input from corticostriatal and thalamostriatal fibres and whether these afferents converge on individual striatal neurons, double anterograde labelling of axon terminals was combined with golgi impregnation at both the light and electron microscopic levels. the area of the central neostriatum that receives input from both the parafascicular nucleus of the thalamus and the somatosensory cortex was identified by re ... | 1988 | 3346370 |
| complex-type glycoproteins synthesized in the subcommissural organ of mammals. light- and electron-microscopic investigations by use of lectins. | the secretory activity in the subcommissural organ (sco) of the sheep and cow was examined by means of lectin histochemistry and cytochemistry. among the various lectins tested. concanavalin a (con a) revealed glycoproteins rich in mannosyl residues in the rough endoplasmic reticulum of ependymal and hypendymal cells. one of these con a-positive glycoproteins may represent the precursor of the specific secretory component elaborated in the sco, giving rise to reissner's fiber. lens culinaris agg ... | 1988 | 3409290 |
| analysis of asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells. | the asparagine-linked oligosaccharide structures of chronic lymphocytic leukemia cells (cll) were studied by sequential lectin affinity chromatography. glycopeptides were isolated from a concanavalin a (cona)-binding glycoprotein fraction prepared from a soluble cll extract. this fraction, which contained 1.8% of the proteins present in the soluble extract, greater than 30 polypeptides revealed by sds-page analysis, and known cell surface antigens such as hla-dr and p85 glycoprotein, must includ ... | 1987 | 3498886 |
| small intestinal mucosal hyperplasia caused by an enterokinase inhibitor from red kidney bean in rats. | a specific enterokinase inhibitor (eki) was purified from red kidney bean (rkb). male weanling rats fed a diet containing this purified eki (0.06%) for 6 d showed increases in mucosal weights, protein, dna and lactic dehydrogenase contents in their small intestines compared to age-matched control rats fed a standard diet. total mucosal ek and disaccharidase activities were, however, decreased in eki-fed rats. thus, oral consumption of eki from rkb led to small intestinal mucosal hyperplasia in r ... | 1986 | 3517252 |
| transcriptional activation of plant defense genes by fungal elicitor, wounding, and infection. | activation of plant defense genes was investigated by analysis of transcripts completed in vitro by isolated nuclei. elicitor treatment of suspension-cultured bean (phaseolus vulgaris l.) cells caused marked transient stimulation of transcription of genes encoding apoproteins of cell wall hydroxyproline-rich glycoproteins (hrgp) and the phenylpropanoid biosynthetic enzymes phenylalanine ammonia-lyase (pal) and chalcone synthase (chs), concomitant with the onset of rapid accumulation of the respe ... | 1987 | 3561393 |
| transplantation of cultured human spinal cord cells into the rat motor cortex: use of phaseolus vulgaris leucoagglutinin as a cell marker. | successful transplantations have been made of cultured explants of human fetal spinal cord into surgically created cavities in the motor cortical area of non-immunosuppressed young adult rats. the cultured cells were marked by brief incubation with phaseolus vulgaris leucoagglutinin (pha) just prior to transplantation. following sacrifice of the rats 1.5 months later, pha immunohistochemistry clearly outlined the demarcation zone of the explants. the transplanted neurons possessed long, somewhat ... | 1987 | 3587750 |
| detailed projection patterns of septal and diagonal band efferents to the hippocampus in the rat with emphasis on innervation of ca1 and dentate gyrus. | the detailed patterns of afferentation to the ammon's horn and dentate gyrus of the hippocampus in the rat were investigated employing the anterograde tracer phaseolus vulgaris leuco-agglutinin (pha-l) after punctate iontophoretic injections in the medial septum (ms) and vertical limb of the diagonal band of broca (vdb). the topographically ordered innervation pattern was different in the regio superior (or ca1) vs. the regio inferior (or ca3) and in the dorsal vs. ventral aspects of ammon's hor ... | 1987 | 3607523 |
| in vitro synthesis and processing of a bean pathogenesis-related (pr4) protein. | when bean plants (phaseolus vulgaris var. saxa) are treated with mercuric chloride or infected with alfalfa mosaic virus, they produce pathogenesis-related (pr) proteins. we report here that functional mrna encoding bean pr4 protein is only present when synthesis of this protein has been induced. treatment with mercuric chloride results in a rapid induction of functional bean pr4 mrna (within 2-3 h), whereas in virus-infected plants this mrna can only be detected the second day following the inf ... | 1987 | 3609026 |
| effect of lectin from phaseolus vulgaris (pha-m) on the glucose content in the blood and the content of glycogen in the mouse liver and muscles. | | 1987 | 3609390 |
| oligosaccharide specificities of phaseolus vulgaris leukoagglutinating and erythroagglutinating phytohemagglutinins. interactions with n-glycanase-released oligosaccharides. | the structural determinants required for interaction of oligosaccharides with leukoagglutinating phytohemagglutinin (l-pha) and erythroagglutinating phytohemagglutinin (e-pha) from phaseolus vulgaris have been studied by immobilized lectin affinity chromatography. homogeneous oligosaccharides of known structure, purified following release from asn with n-glycanase and reduction with nabh4, were tested for their ability to interact with columns of l- and e-pha-agarose. the characteristic elution ... | 1987 | 3624245 |
| [chemical composition and evaluation of the protein quality of beans in adult humans by the short-term nitrogen balance method]. | three varieties of common beans were chemically analyzed and their protein quality was evaluated in 12 human male adult subjects by the short-term nitrogen balance method. chemical analyses were performed for both raw and cooked grains. cooking reduced the trypsin inhibitors in 28 to 73%, hemagglutinins, 100%, tannins as acid tannic equivalents, 9 to 72%, and 55 to 75% as catequin equivalents, and alkali-soluble nitrogen in 65%. red and black beans contained more residual trypsin inhibitors and ... | 1986 | 3632200 |
| a human auto-immune antibody specifically recognizing initiator methionine trna from yeast and higher eucaryotes. | analysis of sera from 168 patients with autoimmune disorders revealed that one patient with sjôgren's syndrome produced antibodies against deproteinized initiator methionine trna in addition to those against la protein. this anti-trnaimet recognizes also trnaimet from yeast but not from phaseolus vulgaris chloroplasts (bean) or e. coli. it appears therefore that the epitope could be located in the tf loop in which an a residue in position 60 and the aucg sequence are the only common features in ... | 1986 | 3639739 |
| [toxicological study of phaseolus vulgaris poisoning]. | | 1987 | 3652860 |
| cultured trabecular-meshwork cells: immunohistochemical and lectin-binding characteristics. | we examined the extracellular matrix formation and lectin-binding properties of cultured trabecular-meshwork cells established from cynomolgus monkey and bovine eyes. using an avidin-biotin complex method, we found that the extracellular matrix in both the monkey and bovine cultures stained intensely with antibodies to fibronectin, type iv collagen, and laminin. these materials were especially prominent when the cultured monkey trabecular-meshwork cells aggregated in clusters, manifesting their ... | 1987 | 3653292 |
| effect of tannic acid on muscle protein composition of chickens fed a raw field bean (vicia faba l.) diet. | | 1987 | 3659550 |
| organization of the projections from the subiculum to the ventral striatum in the rat. a study using anterograde transport of phaseolus vulgaris leucoagglutinin. | the projections of the subiculum, as the main output structure of the hippocampal formation, to the striatum were studied in the rat using the anterograde tracer phaseolus vulgaris leucoagglutinin. it appears that not only the entire nucleus accumbens, part of the so-called ventral striatum, receives fibres from the subiculum, but that the hippocampal projection area in the striatum includes also the most medial, ventral, rostral and caudal parts of the caudate-putamen complex. moreover, a relat ... | 1987 | 3683859 |
| a direct neural projection from the nucleus of the solitary tract to the subfornical organ in the rat. | a direct neuronal projection from the nucleus of the solitary tract (nts) to the subfornical organ (sfo) in rats was demonstrated using the phaseolus vulgaris leucoagglutinin (pha-l) anterograde tracing technique. small deposits of pha-l were iontophoretically placed within the caudal parts of the nucleus of the solitary tract. brain sections were processed for visualization of pha-l using the avidin-biotin immunoperoxidase technique. pha-l immunoreactive beaded axons were observed within the su ... | 1987 | 3683974 |
| efferent projections from posteroventral cochlear nucleus to lateral superior olive in guinea pig. | phaseolus vulgaris leucoagglutinin (pha-l), a kidney bean lectin used as an anterograde tracer, was iontophoretically injected into the posteroventral cochlear nucleus (pvcn) of guinea pigs. pha-l-labeled fiber segments and their terminal specializations were observed within the ipsilateral lateral superior olive (lso) thereby establishing the existence of an efferent neural pathway from pvcn to this nucleus. furthermore, the pathway is topographically organized with dorsal regions of pvcn proje ... | 1987 | 3690282 |
| binding of navy bean (phaseolus vulgaris) lectin to the intestinal cells of the rat and its effect on the absorption of glucose. | the main objectives of this investigation were to study the binding of a lectin from navy beans with the epithelial cells of the rat intestine and to assess the effect of such binding on the ability of the intestine to absorb glucose. a scatchard plot, based on the binding of 125i-labeled lectin to isolated intestinal epithelial cells, was used to calculate an association constant (ka) of 15 x 10(6)m-1 and the number of binding sites per cell, 12 x 10(6). metabolic studies were conducted over a ... | 1987 | 3694292 |
| an economic anterograde axonal tracing method using phaseolus vulgaris agglutinin (pha) p-form. | phaseolus vulgaris leucoagglutinin (pha-l) has been demonstrated to be an excellent neuroanatomical anterograde tracer. so far there are relatively few applications of this technique, mainly due to the cost of the lectin. instead of pha-l, we have successfully used pha-p, which is a crude and inexpensive form of pha-l. the sensitivity of the present method, examined in the rat striatonigral pathway, is as high as that of the conventional method. furthermore, a large amount of pha-p, injected int ... | 1987 | 3695565 |
| light microscopic evidence of striatal input to intrapallidal neurons of cholinergic cell group ch4 in the rat: a study employing the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l). | injections of the anterograde tracer phaseolus vulgaris leucoagglutinin (pha-l) were placed in various striatal loci in the rat. within the globus pallidus, pha-l-filled striatofugal axons were seen to approach cholinergic neurons, identified with either acetylcholinesterase histochemistry or choline acetyltransferase immunohistochemistry, and, apparently, to contact the surface of such cells with axonal varicosities. since these varicosities are thought to mark the sites of synaptic terminals, ... | 1986 | 3697714 |
| the projections of the dorsomedial hypothalamic nucleus in the rat. | the dorsomedial hypothalamic nucleus (dmh) output pathways are revealed by using autoradiographic tracing of tritium labeled leucine and by the recently introduced phaseolus vulgaris leuco-agglutinin immunocytochemical method. terminal labeling appears in the dorsal motor nucleus of the vagus, nucleus ambiguus and in the parvocellular reticular formation at the lower medullary level. mesencephalic labeling is found in the periaqueductal gray at the level of the oculomotor nucleus. in the hypotha ... | 1986 | 3697791 |
| cell surface carbohydrate of leishmania mexicana amazonensis: differences between infective and non-infective forms. | the cell surface carbohydrates of leishmania mexicana amazonensis (amastigotes and promastigotes, both infective and non-infective forms) were comparatively analyzed by agglutination assay employing 28 highly purified lectins, and by binding assay using 125i-labeled lectins. among the d-galnac binding lectins, bandeiraea simplicifolia-i, dolichos biflorus, phaseolus vulgaris and glycine max were highly specific for the amastigotes, while that from maclura aurantiaca selectively agglutinated prom ... | 1986 | 3709546 |
| the projection from the parataenial thalamic nucleus, as demonstrated by the phaseolus vulgaris-leucoagglutinin (pha-l) method, identifies a subterritorial organization of the ventral striatum. | the thalamic projection to the ventral striatum was examined in the rat by immunohistochemistry after iontophoretic injections of phaseolus vulgaris-leucoagglutinin (pha-l) into the parataenial thalamic nucleus. a continuous dense terminal field was observed in the nucleus accumbens and the striatal cell bridges, as well as in the adjoining striatal parts of the olfactory tubercle. these observations provide further evidence of the relevance of the ventral striatal concept. | 1986 | 3719343 |
| the effect of a "bisecting" n-acetylglucosaminyl group on the binding of biantennary, complex oligosaccharides to concanavalin a, phaseolus vulgaris erythroagglutinin (e-pha), and ricinus communis agglutinin (rca-120) immobilized on agarose. | the effect of a "bisecting" 2-acetamido-2-deoxy-beta-d-glucopyranosyl group, linked (1----4) to the beta-d-mannopyranosyl group of asparagine-linked complex and hybrid oligosaccharides, on the binding of [14c]acetylated glycopeptides to columns of immobilized concanavalin a (con a), phaseolus vulgaris erythroagglutinin (e-pha), and ricinus communis agglutinin-120 (rca-120) was investigated. the presence of this "bisecting" glcnac group caused significant inhibition of the binding to cona-agarose ... | 1986 | 3731182 |
| central projections of identified, unmyelinated (c) afferent fibers innervating mammalian skin. | unmyelinated (c) fibers are the most numerous sensory elements of mammalian peripheral nerve and comprise many of those responsible for initiating pain and temperature reactions; however, direct evidence has been lacking as to where and how these fibers terminate in the central nervous system. a plant lectin (phaseolus vulgaris leukoagglutinin) was applied intracellularly by iontophoresis as an immunocytochemical marker. this permitted visualization of the central terminations of cutaneous c sen ... | 1986 | 3764416 |
| differential accumulation of plant defense gene transcripts in a compatible and an incompatible plant-pathogen interaction. | phenylalanine ammonia-lyase and chalcone synthase catalyze the first reaction of phenylpropanoid biosynthesis and the first reaction of a branch pathway specific for flavonoid-isoflavonoid biosynthesis, respectively. these enzymes are key control elements in the synthesis of kievitone, phaseollin, and related isoflavonoid-derived phytoalexins. rna blot hybridization with 32p-labeled cdna sequences was used to demonstrate marked accumulation of phenylalanine ammonia-lyase and chalcone synthase mr ... | 1986 | 3785174 |
| effect of diets containing dry beans (phaseolus vulgaris, l.) on the rat excretion of endogenous nitrogen. | wistar rats of 60.5 +/- 5.0 g fasted for 24 h were injected intraperitoneally with 10 microci of [14c]glycine. one to two hours after injection the rats were fed a diet containing 10.53 +/- 0.75% protein provided by dry beans or casein, or a protein-free diet and submitted to a 4-d nitrogen balance. radioactivity in the feces of rats fed casein, cooked beans and raw beans was roughly 2, 5 and 10 times greater, respectively, than in the feces of those fed the protein-free diet. apparent protein d ... | 1986 | 3806237 |
| the influence of rat endogenous nitrogen excretion on the assessment of bean protein quality. | the main objective of the present work was to study the interference of rat endogenous nitrogen excretion with the assessment of digestibility and biological value of dry bean (phaseolus vulgaris, l.) protein. dry bean plants were cultivated under (15nh4)2so4 fertilization and at harvest dry beans had 1.080 atoms % of 15n-excess. nitrogen balance studies indicated that bean protein digestibility and biological value were higher when n-balance was based on 15n-excess as compared to total nitrogen ... | 1986 | 3806256 |
| effect of lectins on tumorigenicity of akr lymphoma cells of varying malignancy. | this study represents an attempt to characterize differences in the cell surfaces of cells of high - and low - malignancy by use of lectins. the effect of concanavalin a (con a), phaseolus vulgaris lectin (pha) and the galactophilic lectin of pseudomonas aeruginosa (pa-i) on cell agglutination, viability and tumorigenicity of two variants of malignancy of akr lymphoma was tested. cell viability was not affected by the lectins. agglutination of the two variants was similar with all lectins and wa ... | 1986 | 3813494 |
| secretion of phytohemagglutinin by monkey cos cells. | the entire coding region of a gene, which encodes a polypeptide of phytohemagglutinin (pha-l), obtained from a library of genomic dna of the common bean phaseolus vulgaris cv. greensleeves, was introduced into the sv40 expression vector pjc119. monkey cos1 cells were transfected with the recombinant clone and the synthesis, glycosylation, and transport of pha-l studied and compared with the normal processes in bean cotyledons. in the bean, phytohemagglutinin is synthesized on the rough endoplasm ... | 1986 | 3816816 |
| functional incorporation and preferred orientation of phytohemagglutinin receptor glycoproteins in phospholipid vesicles. | porcine lymphocyte phaseolus vulgaris phytohemagglutinin (pha) receptor glycoproteins purified by affinity chromatography have been reassembled into vesicles made of phosphatidylcholine and phosphatidylserine by detergent (dodecyltrimethylammonium bromide) dialysis. the receptor glycoproteins were incorporated into the lipid vesicles in a nonselective manner with a yield of 65-70%. vesicles containing the glycoproteins were sealed as evidenced by their impermeability to calcium ions, using quin ... | 1987 | 3828112 |
| the afferent connections of the substantia innominata in the monkey, macaca fascicularis. | the afferent connections of the substantia innominata and the magnocellular nuclei within it (the nucleus of the horizontal limb of the diagonal band, nhdb, and the nucleus basalis of meynert, nbm) have been studied with anterograde and retrograde axonal tracing techniques. prominent inputs arise in the amygdaloid complex, restricted areas of the cerebral cortex, parts of the thalamus and hypothalamus, and nuclei of the lower brainstem. autoradiographic tracing experiments indicate that the amyg ... | 1985 | 3841131 |