Publications

TitleAbstractYear
Filter
PMID
Filter
disseminated papulopustular eruption due to mycobacterium fortuitum in an immunocompetent patient. 199910825072
peritonitis due to mycobacterium fortuitum infection following gastric cancer surgery.mycobacterium fortuitum is a well-documented cause of nosocomial infection. however, no studies have reported peritonitis with m. fortuitum as a postoperative complication. we describe a case of peritonitis with m. fortuitum biovariant peregrinum following gastric cancer surgery. gram-positive bacterial infection coexisted. although the source of the infection was unclear, the patient was successfully treated with drainage tube exchange and combination therapy consisting of sparfloxacin, clarith ...199910526951
acute bronchopneumonia associated with mycobacterium fortuitum infection in a dog.mycobacterium fortuitum was isolated in a sample of bronchial fluid collected by transtracheal aspiration from a 1-year-old corgi dog with a productive cough of 10 days' duration and with radiographic and cytological features of acute suppurative bronchopneumonia. the dog responded favourably to intravenous gentamicin and cephalexin for three days and a six week course of oral ciprofloxacin. saprophytic mycobacterial pneumonia should be considered in cases of severe pulmonary consolidation in yo ...200010840571
mycobacterium fortuitum infection following neck liposuction: a case report. 200010848943
a kinetic study on the interaction between tazobactam (a penicillanic acid sulphone derivative) and active-site serine beta-lactamases.the interaction between tazobactam and several chromosome- and plasmid-encoded (tem, shv, pse types) class a and c beta-lactamases was studied by spectrophotometry. tazobactam behaved as a competitive inhibitor or inactivator able to restore in several cases the efficiency of piperacillin as a partner beta-lactam. a detailed kinetic analysis permitted measurement of the acylation efficiency for some cephalosporinases and broad-spectrum beta-lactamases; the presence of a turn-over of acyl-enzyme ...200010850951
ventral hernia synthetic mesh repair infected by mycobacterium fortuitum.we report the occurrence of a refractory infection caused by the "rapidly growing" nontuberculous mycobacterium, mycobacterium fortuitum, after incisional hernia repair using synthetic mesh. the patient had previously undergone three herniorrhaphies incorporating polypropylene mesh. multiple surgical debridements were required, along with complete removal of all the mesh, to eradicate the infection. prolonged antimicrobial therapy with sulfamethoxazole, an agent active against the patient's isol ...199910551752
massive hemoptysis due to mycobacterium fortuitum infection controlled with bronchial artery embolization - a case report.we reported a case of first reported pulmonary infection due to mycobacterium fortuitum (m. fortuitum) with massive hemoptysis, successfully treated by bronchial artery embolization (bae). a 78-year-old male was admitted to our hospital complaining of massive hemoptysis. a biochemical examination and dna/dna hybridization revealed m. fortuitum in the culture of his sputum. he was treated by bae, and antituberculous agents and levofloxacin. the patient remains well without recurrence more than on ...199910899418
peritoneal catheter exit-site infections caused by rapidly-growing atypical mycobacteria. 200010978410
mycobacterium fortuitum peritonitis in two patients receiving continuous ambulatory peritoneal dialysis.we present two cases of non-resolving peritonitis treated with a standard peritonitis protocol. the organism identified from the peritoneal effluent was mycobacterium fortuitum, a group iv (runyon's classification) rapidly growing, nontuberculous mycobacterium. m. fortuitum is ubiquitous and can be isolated from a number of natural sources. risk factors these two patients had for developing m. fortuitum peritonitis included underdialysis, the immunocompromised state associated with end stage ren ...199910575188
cloning, expression, and characterization of the katg gene, encoding catalase-peroxidase, from the polycyclic aromatic hydrocarbon-degrading bacterium mycobacterium sp. strain pyr-1.a 81-kda protein from mycobacterium sp. strain pyr-1 was expressed in response to exposure of the strain to the polycyclic aromatic hydrocarbon pyrene and recovered by two-dimensional gel electrophoresis. the n-terminal sequence of the protein indicated that it was similar to catalase-peroxidase. an oligonucleotide probe designed from this sequence was used to screen a genomic library of mycobacterium sp. strain pyr-1, and a positive clone, containing a part of the gene encoding the 81-kda prote ...200011010873
microbial degradation of the multiply branched alkane 2,6,10,15,19, 23-hexamethyltetracosane (squalane) by mycobacterium fortuitum and mycobacterium ratisbonense.among several bacterial species belonging to the general gordonia, mycobacterium, micromonospora, pseudomonas, and rhodococcus, only two mycobacterial isolates, mycobacterium fortuitum strain nf4 and the new isolate mycobacterium ratisbonense strain sd4, which was isolated from a sewage treatment plant, were capable of utilizing the multiply branched hydrocarbon squalane (2,6,10,15,19, 23-hexamethyltetracosane) and its analogous unsaturated hydrocarbon squalene as the sole carbon source for grow ...200011010899
mycobacterium thermoresistible recovered from a cutaneous lesion in an otherwise healthy individual.this is the first report of coinfection by mycobacterium thermoresistible and mycobacterium fortuitum and only the fifth case of human infection by m. thermoresistible reported in the world literature.200011017838
susceptibility testing for mycobacteria.mycobacterial susceptibility testing is important for the management of patients with tuberculosis and those with disease caused by certain nontuberculous mycobacteria. to help standardize methods used in the clinical microbiology laboratory for testing susceptibility of mycobacteria, the national committee for clinical laboratory standards (nccls) recently updated nccls document m24-t (published in 1995), which is the tentative standard for antimycobacterial susceptibility testing of mycobacter ...200011073754
skin and wound infection by rapidly growing mycobacteria: an unexpected complication of liposuction and liposculpture. the venezuelan collaborative infectious and tropical diseases study group.to describe 10 patients with skin and soft tissue infection caused by rapidly growing mycobacteria after cosmetic liposuction and liposculpture.200011074697
[rapidly growing mycobacteria in human pathology]. 200011075485
urinary mycobacterium fortuitum infection in an hiv-infected patient. 200011125907
induction of neutralizing antibodies against diphtheria toxin by priming with recombinant mycobacterium bovis bcg expressing crm(197), a mutant diphtheria toxin.bcg, the attenuated strain of mycobacterium bovis, has been widely used as a vaccine against tuberculosis and is thus an important candidate as a live carrier for multiple antigens. with the aim of developing a recombinant bcg (rbcg) vaccine against diphtheria, pertussis, and tetanus (dpt), we analyzed the potential of crm(197), a mutated nontoxic derivative of diphtheria toxin, as the recombinant antigen for a bcg-based vaccine against diphtheria. expression of crm(197) in rbcg was achieved usi ...200111159980
aminoglycoside resistance in mycobacterium kansasii, mycobacterium avium-m. intracellulare, and mycobacterium fortuitum: are aminoglycoside-modifying enzymes responsible?aminoglycoside acetyltransferase was detected in mycobacterium kansasii and m. fortuitum but not in m. avium-m. intracellulare when they were screened by a radioassay. aminoglycoside phosphotransferase and nucleotidyltransferase activities were absent from all three species tested. acetyltransferases from both m. kansasii and m. fortuitum displayed relatively high k(m)s, all at the millimolar level, for substrates including tobramycin, neomycin, and kanamycin a. the k(m) of each substrate was we ...200010602720
insect wing case: unusual foreign body.to report five cases of insect wing case foreign body.200111188995
diagnosis and management of atypical mycobacterium spp. infections in established laboratory zebrafish (brachydanio rerio) facilities.two established zebrafish colonies experienced increased mortality and decreased reproductive performance. initial examination of several fish from one facility revealed hyperemic gills, petechia around the opercula, abdominal distention, and emaciation. affected fish had congested liver with inflammation and multifocal hepatic necrosis. large numbers of acid-fast-positive, rod-shaped bacteria were evident in multiple tissues and the blood. mycobacterium fortuitum was subsequently isolated from ...200011200575
quinazoline derivatives with antitubercular activity.4-quinazolinol was prepared by the reaction of anthranilic acid and formamide. the hydroxy group was converted into the thiol function by treatment with phosphorus(v)sulfide, and the subsequent alkylation of the thiol group was carried out with alkylhalides under the conditions of phase-transfer catalysis. the structure of the substances was confirmed by 1h, 13c nmr, ir, and ms. most of the synthesized compounds exhibited antimycobacterial activity against the strains of mycobacterium tuberculos ...200011204949
in vitro activity of a novel antimycobacterial compound, n-octanesulfonylacetamide, and its effects on lipid and mycolic acid synthesis.beta-sulfonyl carboxamides have been proposed to serve as transition-state analogues of the beta-ketoacyl synthase reaction involved in fatty acid elongation. we tested the efficacy of n-octanesulfonylacetamide (osa) as an inhibitor of fatty acid and mycolic acid biosynthesis in mycobacteria. using the bactec radiometric growth system, we observed that osa inhibits the growth of several species of slow-growing mycobacteria, including mycobacterium tuberculosis (h37rv and clinical isolates), the ...200111257028
mycobactericidal activity of selected disinfectants using a quantitative suspension test.in this study, a quantitative suspension test carried out under both clean and dirty conditions was used to assess the activity of various instrument and environmental disinfectants against the type strain nctc 946 and an endoscope washer disinfector isolate of mycobacterium chelonae, mycobacterium fortuitum nctc 10,394, mycobacterium tuberculosis h37 rv nctc 7416 and a clinical isolate of mycobacterium avium-intracellulare (mai). the disinfectants tested were; a chlorine releasing agent, sodium ...199910063473
mycobacterium fortuitum-chelonae complex infection in a child with complete interleukin-12 receptor beta 1 deficiency.a 10-year-old boy had chronic diarrhea, abdominal pain, severe weight loss and hepatomegaly; multiple enlarged para-aortic and mesenteric lymph nodes. mycobacterium fortuitum-chelonae complex was identified in the culture of the lymph nodes. interleukin-12 receptor beta 1 expression could not be observed in phytohemagglutinin-driven t cell blasts. a homozygous missense interleukin-12 receptor beta 1 mutation was found (r173p).200111368122
mycobacterial infections of the head and neck.mycobacteria are important causes of head and neck infections. mycobacterial lymphadenitis may be caused by both mycobacterium tuberculosis and a variety of nontuberculous myocbacteria. changes in the epidemiology of tuberculosis have caused a shift of the peak age range of tuberculous lymphadenitis from childhood to ages 20 to 40 years. short-course chemotherapy is highly effective. mycobacterium avium has become the most common cause of nontuberculous lymphadenitis, but new mycobacterial speci ...200111384553
a comparison of the bactericidal activity of quinolone antibiotics in a mycobacterium fortuitum model.new agents are urgently needed to meet the threat of multiple drug-resistant tuberculosis and to manage infection with the naturally resistant non-tuberculosis mycobacteria. earlier fluoroquinolones have been shown to have promising in-vitro activity, although mouse infection and clinical studies suggested that they lack sufficient bactericidal activity. methods were evaluated to measure the bactericidal activity of fluoroquinolones and to compare the new agent moxifloxacin with other fluoroquin ...200111393294
[comparison of in vitro antimicrobial activities of ofloxacin, levofloxacin, ciprofloxacin, and sparfloxacin against various mycobacteria].in vitro antimicrobial activities of ofloxacin (oflx), levofloxacin (lvfx), ciprofloxacin (cpfx), and sparfloxacin (spfx) were compared against various mycobacteria using the agar dilution method with 7h11 medium, and the following results were obtained. (1) these four new quinolones showed excellent antimicrobial activities against m. tuberculosis, m. kansasii, and m. fortuitum. (2) spfx was most active against slowly growing mycobacteria. the activity against m. tuberculosis was in the order o ...200111398326
non tuberculous mycobacterium peritonitis in continuous ambulatory peritoneal dialysis.a forty-five-year old saudi lady who had been on continuous ambulatory peritoneal dialysis (capd) for three years, was admitted with a clinical picture of night fever, sweating, weight loss and turbid peritoneal fluid (pf). the pf had a high cell count, predominantly neutrophils. this condition failed to respond to a standard vancomycin-gentamycin treatment, and acid fast bacilli (afbs) were stained from the pf. the patient was commenced on antituberculous treatment and the tenckhoff catheter wa ...200111411016
some 3-thioxo/alkylthio-1,2,4-triazoles with a substituted thiourea moiety as possible antimycobacterials.a series of novel n-alkyl/aryl-n'-[4-(4-alkyl/aryl-2,4-dihydro-3h-1,2,4-triazole-3-thione-5-yl)phenyl]thioureas 1-19 and three s-alkylated representatives of the former, n-alkyl/aryl-n'-[4-(3-aralkylthio-4-alkyl/aryl-4h-1,2,4-triazole-5-yl)phenyl]thioureas 20-22, were synthesized and tested for antimycobacterial activity against mycobacterium tuberculosis h37rv as well as mycobacterium fortuitum atcc 6841 which is a rapid growing opportunistic pathogen. compounds 4 and 9-11 were found to possess ...200111425542
partial characterization of a major autolysin from mycobacterium phlei.autolytic enzyme profiles of fast- and slow-growing mycobacteria were examined using sds-page zymography with incorporated mycobacterial peptidoglycan sacculi as substrate. each species tested (mycobacterium phlei, mycobacterium smegmatis, mycobacterium aurum, mycobacterium fortuitum and mycobacterium kansasii) appeared to produce a different set of enzymes on the basis of differing number and molecular masses. a major autolysin from m. phlei was purified to apparent homogeneity by deae-cellulos ...199910206696
pathogenicity of mycobacterium fortuitum and mycobacterium smegmatis to goldfish, carassius auratus.despite the ubiquitous presence of atypical mycobacteria in the environment and the potential risk of infection in humans and animals, the pathogenesis of diseases caused by infection with atypical mycobacteria has been poorly characterized. in this study, goldfish, carassius auratus were infected either with the rapidly growing fish pathogen, mycobacterium fortuitum or with another rapidly growing mycobacteria, mycobacterium smegmatis. bacterial persistence and pathological host response to myc ...199910227476
[evaluation of rapidly growing mycobacteria isolates in a general hospital: reports from the hospital microbiology laboratory].forty isolates of rapidly growing mycobacteria, mycobacterium fortuitum group including m. fortuitum and m. peregrinum and m. chelonae group including m. chelonae subsp. chelonae and m. chelonae subsp. abscessus at showa university fujigaoka hospital collected between february 1981 and december 1997 were investigated in this study. these isolates were from the patients who were not infected with hiv. the average age of fourteen patients, from whom m. fortuitum group was isolated, was 58 years, r ...200111449697
multisite reproducibility of results obtained by the broth microdilution method for susceptibility testing of mycobacterium abscessus, mycobacterium chelonae, and mycobacterium fortuitum.a multicenter study was conducted to assess the interlaboratory reproducibility of broth microdilution testing of the more common rapidly growing pathogenic mycobacteria. ten isolates (four mycobacterium fortuitum group, three mycobacterium abscessus, and three mycobacterium chelonae isolates) were tested against amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, sulfamethoxazole, and tobramycin (m. chelonae only) in four laboratories. at each site, isolates were tested t ...199910325306
hospital water supply as a source of disseminated mycobacterium fortuitum infection in a leukemia patient.nosocomial acquisition of mycobacterium fortuitum led to a disseminated infection in a leukemia patient. a linkage to showerhead water was supported by molecular typing of clinical and environmental isolates. contamination of the hospital water system with microbes that are relatively resistant to common sanitation processes poses an increased risk of infection to neutropenic patients.199910349952
clinical and pathologic features of mycobacterium fortuitum infections. an emerging pathogen in patients with aids.the clinical and pathologic features of mycobacterium fortuitum infection in 11 patients with aids were characterized. nine patients had cervical lymphadenitis; 2 had disseminated infection. the infection occurred late in the course of aids, and the only laboratory abnormality seen in more than half of patients (7/11) was relative monocytosis. absolute monocytosis also was seen in 4 of 11 patients. in both cytologic and histologic preparations, the inflammatory pattern was suppurative with necro ...200111488069
treatment of mycobacterial exit-site infections in patients on continuous ambulatory peritoneal dialysis.exit-site infections (esis) are frequently due to gram-positive organisms and occasionally to gram-negative organisms. initial empiric antibiotic therapy is therefore directed against these organisms until culture reports are available. two cases of esi associated with mycobacterium are here reported. the first patient, a 63-year-old man with type 2 diabetes, recently treated for staphylococcus epidermidis peritonitis, presented with acute purulent drainage at the catheter exit site, accompanied ...200111510269
mycobacterium fortuitum wound infection following laparoscopy.during a six week period in 1999, seven patients who underwent laparoscopic tubectomies at small town health centres near chandigarh developed chronic discharging sinuses at the site of incision. mycobacterium fortuitum was isolated from wound discharge of the five patients by standard methods and two patients were smear positive. environmental samples e.g., tap water, and a variety of fluids did not yield any mycobacteria and swabs from different parts of the laparoscope were sterile. all patie ...200111525156
multisite reproducibility of etest for susceptibility testing of mycobacterium abscessus, mycobacterium chelonae, and mycobacterium fortuitum.a multicenter study was conducted to assess the inter- and intralaboratory reproducibility of the etest for susceptibility testing of the rapidly growing mycobacteria. the accuracy also was evaluated by comparing etest results to those obtained by broth microdilution. ten isolates (four of the mycobacterium fortuitum group, three of mycobacterium abscessus, and three of mycobacterium chelonae) were tested against amikacin, cefoxitin, ciprofloxacin, clarithromycin, doxycycline, imipenem, and trim ...200010655363
screening of seven selected rwandan medicinal plants for antimicrobial and antiviral activities.aqueous etoh (80%) extracts of seven plants used by rwandan traditional healers to treat infections, were screened for antibacterial, antifungal, and antiviral activities. only two of the selected plants showed a true antiviral activity against herpes simplex virus type 1, while all of them exhibited virucidal properties against several enveloped viruses including herpes simplex, measles, semliki forest, and vesicular stomatitis viruses. four plants were diversely active against gram-positive ba ...199910350370
rapid identification of cutaneous infections by nontubercular mycobacteria by polymerase chain reaction-restriction analysis length polymorphism of the hsp65 gene.nontubercular mycobacteria (ntm) may cause cutaneous infections which are difficult to interpret due to the variability of the clinical manifestations. this study involved eight patients (four men and four women) with primary cutaneous infections caused by ntm; the skin lesions included dermo-hypodermal abscesses, suppurative granulomas, and papulonodules localized on the legs, arms, hands, and face. the general condition of the patients was relatively good and they were not immunosuppressed.200111703519
clustering of cases of mycobacterium fortuitum infection investigated by molecular typing.organisms of the mycobacterium fortuitum complex are recognised but uncommon causes of pulmonary disease, primary cutaneous disease and a wide spectrum of nosocomial infections. m fortuitum was isolated from 20 patients over a 15 month period, with an apparent clustering of isolates occurring from january to march 1994. the molecular epidemiology of this clustering was investigated using an arbitrary primer polymerase chain reaction method (ap-pcr). 21 isolates were studied, which yielded 13 dis ...199910375985
infection of the subcutis and skin of cats with rapidly growing mycobacteria: a review of microbiological and clinical findings.mycobacteria were isolated and characterised from 49 cats with extensive infections of the subcutis and skin. cats were generally between 3 and 10 years of age, and female cats were markedly over-represented. all isolates were rapid-growers and identified as either mycobacteria smegmatis (40 strains) or m fortuitum (nine strains). on the basis of etest for minimum inhibitory concentration and/or disc diffusion susceptibility testing, all strains of m smegmatis were susceptible to trimethoprim wh ...200011716590
cutaneous infection with mycobacterium fortuitum after localized microinjections (mesotherapy) treated successfully with a triple drug regimen.mesotherapy is a treatment method devised for controlling pain syndromes or diseases by subcutaneous microinjections given at or around the involved areas at short intervals of time. different adverse effects have been described due to this modality of treatment. this report describes 3 patients with cutaneous infection caused by mycobacterium fortuitum after mesotherapy. three women, aged 24, 27 and 44 years, presented with similar clinical features, consisting of painful nodules located at the ...200111720180
novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16s-23s rrna gene spacer and restriction endonucleases.a novel genus-specific pcr for mycobacteria with simple identification to the species level by restriction fragment length polymorphism (rflp) was established using the 16s-23s ribosomal rna gene (rdna) spacer as a target. panspecificity of primers was demonstrated on the genus level by testing 811 bacterial strains (122 species in 37 genera from 286 reference strains and 525 clinical isolates). all mycobacterial isolates (678 strains among 48 defined species and 5 indeterminate taxons) were amp ...200010699003
[clinical analysis of 96 cases with pulmonary disease caused by nontuberculous mycobacteria].to study the clinical characteristics of nontuberculous mycobacteria (ntm) pulmonary disease.199911775923
failure of the mycobacterium bovis bcg vaccine: some species of environmental mycobacteria block multiplication of bcg and induction of protective immunity to tuberculosis.the efficacy of mycobacterium bovis bacillus calmette-guérin (bcg) vaccine against pulmonary tuberculosis (tb) varies enormously in different populations. the prevailing hypothesis attributes this variation to interactions between the vaccine and mycobacteria common in the environment, but the precise mechanism has so far not been clarified. our study demonstrates that prior exposure to live environmental mycobacteria can result in a broad immune response that is recalled rapidly after bcg vacci ...200211796598
development of tuberculin reactivity and sensitization to m. scrofulaceum and m. fortuitum in children bcg-vaccinated at birth.since the incidence of tuberculosis is steadily declining in finland and infections by environmental mycobacteria may be increasing, the aim of the present study was to evaluate the development of tuberculin reactivity and sensitization to environmental mycobacteria. healthy finnish schoolchildren aged 10.4-12.4 yrs (n=201) were tested with tuberculin purified protein derivative rt23, mycobacterium scrofulaceum rs95 and m. fortuitum rs20 sensitins. the same children had been previously tested wi ...200010706508
further evaluation of rwandan medicinal plant extracts for their antimicrobial and antiviral activities.a total of 45 rwandan plant extracts, belonging to 37 different plant species out of 21 families, were investigated for their antibacterial, antifungal, and antiviral properties. the plants were selected on the base of their ethnomedicinal use against infections and autoimmune diseases. from all the plant extracts tested, only clematis hirsuta (leaves) showed a pronounced antifungal activity against candida albicans and the dermatophytes trichophyton rubrum, epidermophyton floccosum, and microsp ...200211801376
characterization of an rrna operon (rrnb) of mycobacterium fortuitum and other mycobacterial species: implications for the classification of mycobacteria.mycobacteria are thought to have either one or two rrna operons per genome. all mycobacteria investigated to date have an operon, designated rrna, located downstream from the mura gene. we report that mycobacteriun fortuitum has a second rrn operon, designated rrnb, which is located downstream from the tyrs gene; tyrs is very close to the 3' end of a gene (3-mag) coding for 3-methylpurine-dna-glycosylase. the second rrn operon of mycobacterium smegmatis was shown to have a similar organization, ...200211807068
non-tuberculous mycobacterial keratitis at the interface after laser in situ keratomileusis.to describe two cases of bacterial keratitis with atypical mycobacteria after laser in situ keratomileusis (lasik).200211828913
a clinico-bacteriological study of peripheral tuberculous lymphadenitis.tuberculous lymphadenitis is the commonest form of extra-pulmonary tuberculosis. it is most often caused by m. tuberculosis though several reports from other countries have shown mycobacteria other than tuberculosis (mott) to be responsible for a significant proportion of tuberculous lymphadenitis cases. the present study was conducted to find the prevalence of m. tuberculosis and mott as aetiological agents in patients with peripheral tuberculous lymphadenitis.200111837469
iatrogenic mycobacterium infection after an epidural injection.case report.199910382255
atypical piscine mycobacteriosis in japanese medaka (oryzias latipes).japanese medaka, (oryzias latipes), small, freshwater, tropical cyprinodonts, are principally used for toxicologic and carcinogenicity assays, but are finding more applications in developmental genetic and biological research. an increase in mortality began in brood stock of adult medaka that had been shipped and housed separately by sex. initially, mortality averaged one fish daily and began in females two weeks after they were received. cohabitation began eight weeks after arrival. after four ...200111922182
degradation of morpholine, piperidine, and pyrrolidine by mycobacteria: evidences for the involvement of a cytochrome p450.nine bacterial strains that grew on morpholine and pyrrolidine as sole carbon, nitrogen, and energy sources were isolated from three different environments with no known morpholine contamination. one of these strains could also degrade piperidine. these bacteria were identified as mycobacterium strains. a phylogenetic analysis based on the partial 16s rdna sequences indicated that the isolated strains clustered within the fast growing group of mycobacteria. when the above-mentioned cyclic amines ...199910408093
[solitary pulmonary nodule due to mycobacterium fortuitum].we report the case of an asthmatic man in whom a solitary pulmonary nodule was discovered after an episode of self-limited hemoptysis. infection was suspected after initial response to empirical antibiotic therapy, and the pathogen was later identified to be a rare mycobacterium. the pulmonary nodule resolved without surgery after oral quinolone therapy. mycobacterium fortuitum should be added to the list of possible causes of solitary pulmonary nodule in spain.200211953274
pyogranulomatous panniculitis in a cat associated with infection by the mycobacterium fortuitum/peregrinum group.a 6-year-old, spayed female, american domestic shorthair was presented with a 10-month history of nodules on the dorsum. diagnosis of pyogranulomatous panniculitis caused by mycobacterium fortuitum/peregrinum group was achieved by using tissue culture, chromatography, and histopathologic examination. pathological findings, diagnosis, and clinical management of the condition are discussed.200211963662
an outbreak of mycobacterial furunculosis associated with footbaths at a nail salon.in september 2000, a physician in northern california described four patients with persistent, culture-negative boils on the lower extremities. the patients had received pedicures at the same nail salon. we identified and investigated an outbreak of mycobacterium fortuitum furunculosis among customers of this nail salon.200211986410
pseudo-outbreak of mycobacterium fortuitum due to contaminated ice machines.an almost 4-fold increase from normal baseline levels in the isolation of mycobacterium fortuitum from respiratory tract specimens was observed. the majority of these isolates were obtained from patients residing on 1 of 2 wards, prompting an epidemiologic investigation.200211988715
[pleural effusion secondary to mycobacterium fortuitum infection]. 200211989108
mycobacterium fortuitum infection in prosthetic breast implants. 200211994623
fluorescence in situ hybridization assay using peptide nucleic acid probes for differentiation between tuberculous and nontuberculous mycobacterium species in smears of mycobacterium cultures.tb pna fish is a new fluorescence in situ hybridization (fish) method using peptide nucleic acid (pna) probes for differentiation between species of the mycobacterium tuberculosis complex (mtc) and nontuberculous mycobacteria (ntm) in acid-fast bacillus-positive (afb+) cultures is described. the test is based on fluorescein-labelled pna probes that target the rrna of mtc or ntm species applied to smears of afb+ cultures for microscopic examination. parallel testing with the two probes serves as ...199910449448
pseudo-outbreak of mycobacterium fortuitum on a human immunodeficiency virus ward: transient respiratory tract colonization from a contaminated ice machine.infection control surveillance revealed an abnormally high number of sputum samples growing mycobacterium fortuitum from a single ward that houses the human immunodeficiency virus service. we investigated the outbreak using a retrospective case-control study and molecular epidemiology. a total of 47 patients were identified with >/=1 sputum sample that grew m. fortuitum. no significant demographic or clinical variables were found to be associated with colonization. environmental investigation de ...200212060872
a new single-copy mycobacterial plasmid, pmf1, from mycobacterium fortuitum which is compatible with the pal5000 replicon.a 9.2 kb cryptic mycobacterium fortuitum plasmid, pmf1, was isolated from strain 110 and its restriction map constructed. a 4.2 kb hindiii fragment of pmf1 was found to support replication in mycobacteria and this fragment was cloned and sequenced to characterize the replication elements of the plasmid. computer analysis identified a putative rep protein (362 amino acids) with high homology to the putative rep protein of the mycobacterium celatum plasmid pclp and limited homology, mostly in the ...200010708368
analysis of the internal replication region of a mycobacterial linear plasmid.linear plasmids have previously been identified by the authors in mycobacteria, the telomeres of which have terminal inverted repeats and covalently attached proteins. in this study, the replication of these unusual molecules was investigated by studying a 25 kb linear plasmid from the slow-growing species mycobacterium celatum called pclp. an internal region of pclp responsible for replication in mycobacterium smegmatis was identified. the nucleotide sequence of the minimum replication region o ...200010708369
effect of pretreatment of rubber material on its biodegradability by various rubber degrading bacteria.the effect of pretreatment of several cis-1,4-polyisoprene containing rubbers on their biodegradability was examined. tests were carried out with six recently isolated and characterized rubber degrading bacteria belonging to the genera gordonia (strains kb2, kd2 and vh2), mycobacterium, micromonospora and pseudomonas. all strains were able to use natural rubber (nr) as well as nr latex gloves as sole carbon source. extraction of nr latex gloves by organic solvents resulted in an enhancement of g ...200010713421
diverse bacteria are pathogens of caenorhabditis elegans.practically and ethically attractive as model systems, invertebrate organisms are increasingly recognized as relevant for the study of bacterial pathogenesis. we show here that the nematode caenorhabditis elegans is susceptible to a surprisingly broad range of bacteria and may constitute a useful model for the study of both pathogens and symbionts.200212117988
purification and inhibition by quinolones of dna gyrases from mycobacterium avium, mycobacterium smegmatis and mycobacterium fortuitum bv. peregrinum.the dna gyrases from mycobacterium avium, mycobacterium smegmatis and mycobacterium fortuitum bv. peregrinum, which are species naturally resistant, moderately susceptible and susceptible to fluoroquinolones, respectively, were purified by affinity chromatography on novobiocin-sepharose columns. the dna gyrase inhibiting activities (ic50 values) of classical quinolones and fluoroquinolones were determined from the purified enzymes and were compared to the corresponding antibacterial activities ( ...199910517605
[nontuberculous mycobacterial infection followed for 12 years].a 67-year-old woman presented in september 1985 with productive cough, bloody sputum, and dyspnea on exertion. productive cough and bloody sputum had developed when the patient was 55 years old. sputum culture and radiologic findings yielded a diagnosis of nontuberculous mycobacteriosis (ntm). antituberculous therapy with inh, rfp, and eb was initiated in november 1987 because of the development of a cavity in the right upper lobe, and led to resolution of the lesion and clinical symptoms. despi ...200010723956
a prosthetic breast implant infected with mycobacterium fortuitum.augmentation mammaplasty is a common operation performed in the united states. postoperative wound infections are rare, but can be devastating. most often, bacteria from the normal skin flora cause these infections, but more atypical organisms can lead to similar situations. the authors present a case of a prosthetic breast implant infected with mycobacterium fortuitum after augmentation mammaplasty. the patient, diagnosis, and treatment are discussed so that others may recognize and treat this ...200010735228
management of hemodialysis catheter-related bacteremia--a 10-year experience.between january 1986 and december 1995, 18 episodes of bacteremia occurred in our pediatric patients undergoing chronic hemodialysis on an outpatient basis. seven episodes were caused by coagulase-negative staphylococcus, 6 by staphylococcus aureus, 2 by mycobacterium, and 1 each by pseudomonas, xanthomonas, and enterococcus. in 6 cases, the catheter was retained with antimicrobial therapy alone, whereas 12 cases required removal of the catheter after some period of time. the subset of cases in ...200010752756
isolation of a novel insertion sequence from mycobacterium fortuitum using a trap vector based on inactivation of a lacz reporter gene.an insertion sequence of mycobacterium fortuitum has been isolated using a trap vector following insertion in and inactivation of the lacz reporter gene. the trap vector is a temperature-sensitive (ts) escherichia coli-mycobacterium shuttle plasmid, pcd4, which contains ts orim, the kanamycin-resistance gene as a selection marker and a lacz expression cassette. the ts mutation present in pcd4 functions in mycobacteria and enables screening for transposable elements from the mycobacterial genome ...200010832643
löwenstein-jensen media. no longer necessary for mycobacterial isolation.isolation of mycobacteria on löwenstein-jensen media (lj) and in the bactec mb9000 (mb) system was compared. of 2,271 specimens, 317 were positive for 331 mycobacteria isolated in 1 or both media. the mb was positive in 238 isolates, and lj was positive for 239 isolates; 92 isolates were detected by mb only and 93 by lj only. of the 331 isolates, 146 were recovered by both media. mb recovered 38 of 38 mycobacterium tuberculosis complex isolates, while lj recovered 23. mb recovered 94.1% (96/102) ...200010874876
comparison of the bactec mgit 960 with löwenstein-jensen medium for recovery of mycobacteria from clinical specimens.taiwan provincial chronic disease control bureau.200010985656
activities of linezolid against rapidly growing mycobacteria.linezolid is an oxazolidinone available as an oral drug which has activity against most gram-positive bacteria. however, few species of the genus mycobacterium have been studied. we tested 249 clinical isolates and 10 reference strains of rapidly growing mycobacteria for susceptibility to linezolid by broth microdilution. clinical species included the mycobacterium fortuitum group (n = 74), m. abscessus (n = 98), m. chelonae (n = 50), m. mucogenicum (n = 10), and m. fortuitum third biovariant co ...200111181357
isolation of strong expression signals of mycobacterium tuberculosis.the natural fluorescence of the aequoria victoria green fluorescent protein was exploited to isolate strong expression signals of mycobacterium tuberculosis. mycobacterium bovis bacille calmette-guérin harbouring m. tuberculosis fragments driving high levels of gfp expression were isolated by fluorescence-activated cell sorting (facs). dna sequencing and subsequent comparison with the m. tuberculosis genome sequence revealed that a total of nine postulated promoters had been identified. the majo ...200111320128
mycobacterial pulmonary infection post allogeneic bone marrow transplantation.allogeneic bone marrow transplant recipients are prone to pulmonary infections caused by a wide spectrum of organisms. since the first bone marrow transplatation (bmt) done in 1983 at the tata memorial hospital, we have recently seen the first case of mycobacterium fortuitum chelonae complex among 117 bmt (including 90 allogeneic and 27 autologous) patients. the patient was on immunosuppressants for chronic gvhd post allogeneic bmt done for cml-cp. he developed pulmonary mycobacterial infection ...200111426541
diptera as vectors of mycobacterial infections in cattle and pigs.mycobacteria were isolated from 14 (4.5%) of 314 samples, containing 7791 adult diptera, which were collected in the czech republic and slovakia in 1997-2000. these flies were collected from three cattle herds with paratuberculosis, two pig herds with mycobacterial infections and one farm that kept both cattle and pigs and that did not have problems of mycobacterial infections. mycobacterium intracellulare was isolated from eristalis tenax linnaeus (diptera: syrphidae) captured from a pig herd. ...200111434556
skin test reactivity to mycobacterial antigens parallels the phylogenetic structure of their genus.city of manaus, amazonas, brazil.200111467372
structures of coenzyme f(420) in mycobacterium species.the structure of coenzyme f(420) in mycobacterium smegmatis was examined using proton nmr, amino acid analysis, and hplc. the two major f(420) structures were shown to be composed of a chromophore identical to that of f(420) from methanobacterium thermoautotrophicum, with a side chain of a ribityl residue, a lactyl residue and five or six glutamate groups (f(420)-5 and f(420)-6). peptidase treatment studies suggested that l-glutamate groups are linked by gamma-glutamyl bonds in the side chain. h ...200111479701
mycobacterium immunogenum sp. nov., a novel species related to mycobacterium abscessus and associated with clinical disease, pseudo-outbreaks and contaminated metalworking fluids: an international cooperative study on mycobacterial taxonomy.pcr-restriction enzyme pattern analysis of a 439 bp hsp65 gene segment identified 113 unique isolates among non-pigmented rapidly growing mycobacteria (rgm) from clinical and environmental sources that failed to match currently recognized species patterns. this group represented 40% of isolates recovered from bronchoscope contamination pseudo-outbreaks, 0% of disease-associated nosocomial outbreaks and 4% of routine clinical isolates of the mycobacterium abscessus/mycobacterium chelonae group su ...200111594606
mycobacterial di-o-acyl-trehalose inhibits mitogen- and antigen-induced proliferation of murine t cells in vitro.2,3-di-o-acyl-trehalose (dat) is a glycolipid located on the outer layer of the mycobacterium tuberculosis cell envelope. due to its noncovalent linkage to the mycobacterial peptidoglycan, dat could easily interact with host cells located in the focus of infection. the aim of the present work was to study the effects of dat on the proliferation of murine spleen cells. dat was purified from reference strains of m. tuberculosis, or m. fortuitum as a surrogate source of the compound, by various chr ...200111687444
subcutaneous infection with mycobacterium fortuitum after allogeneic bone marrow transplantation.reports of cases of mycobacterial infections after sct are rare. we report a 30-year-old female with a cutaneous infection of mycobacterium fortuitum 30 months after allogeneic bone marrow transplantation for acute lymphoblastic leukemia. the patient was successfully treated with surgical debridement followed by oral minocycline and clarithromycin. mycobacterial infections should be considered in sct patients with undiagnosed refractory chronic cutaneous infection, and surgical debridement is us ...200111704796
selection of mycobacterium sp. strains with capacity to biotransform high concentrations of beta-sitosterol.in this work, phytosterol-biotransforming strains were selected from mycobacterium sp., using a high concentration of beta-sitosterol. the selection was made by culturing the strains in a medium enriched with 14 g beta-sitosterol/l as the unique source of carbon. during 2 months, the bacterial cultures were transferred successively. the extraction of the biotransformation products was made with methanol and ethyl acetate. the qualitative and quantitative analysis was made by means of thin-layer ...200111759690
mycobacterium mucogenicum isolated from a patient with granulomatous hepatitis.mycobacterium mucogenicum is a recently characterized, rapid-growing mycobacteria rarely seen in human infections. we describe the case of a 51-year-old man with rapidly progressive granulomatous hepatitis caused by m. mucogenicum. although premortem evaluation failed to identify an etiologic agent, autopsy liver cultures produced smooth, rapid-growing mycobacterial colonies. biochemical, growth, and cell wall fatty acid characteristics were consistent with the identification of m. mucogenicum.200211800651
chlorine disinfection of atypical mycobacteria isolated from a water distribution system.we studied the resistance of various mycobacteria isolated from a water distribution system to chlorine. chlorine disinfection efficiency is expressed as the coefficient of lethality (liters per minute per milligram) as follows: mycobacterium fortuitum (0.02) > m. chelonae (0.03) > m. gordonae (0.09) > m. aurum (0.19). for a c.t value (product of the disinfectant concentration and contact time) of 60 mg.min.liter(-1), frequently used in water treatment lines, chlorine disinfection inactivates ov ...200211872446
mycobacterium fortuitum complex endocarditis-case report and literature review.endocarditis due to mycobacterium fortuitum complex is a rare entity generally linked to the hospital environment. only 18 cases have been published since 1966. here we present a case of a female who developed an endocarditis due to mycobacterium chelonae after valve replacement as well as a review of the literature. the course of this kind of endocarditis is generally subacute and the outcome is usually fatal. blood cultures were positive in 75% of cases of metallic valve endocarditis, versus 2 ...200211952729
rapidly growing members of the genus mycobacterium affecting dogs and cats.rapidly growing members of the genus mycobacterium were most often associated with chronic (2 to 72 months), nonhealing skin lesions of dogs and cats. mycobacterium fortuitum (m. fortuitum) was the most commonly isolated mycobacterium obtained from these lesions, although m. chelonae-abscessus and m. flavescens were occasionally encountered. isolates were tested in vitro to various antimicrobial agents and found to be susceptible to amikacin (100% of the isolates), cefoxitin (93.8%), ciprofloxac ...200212022405
trehalose-based oligosaccharides isolated from the cytoplasm of mycobacterium smegmatis. relation to trehalose-based oligosaccharides attached to lipid.a series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of mycobacterium smegmatis and purified by gel-filtration and paper chromatography and tlc. their structures were determined by hplc and glc to determine sugar composition and ratios, maldi-tof ms to measure molecular mass, methylation analysis to determine linkages, (1)h-nmr to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by tlc to determine sequences and ano ...200212084054
cutaneous mycobacterium fortuitum infection mimicking lupus vulgaris.we report a woman with a lupus vulgaris-like skin eruption caused by mycobacterium fortuitum. the presence of mycobacteria was confirmed with tissue culture and also the detection of mycobacterial heat shock protein 65 (hsp65) dna in the biopsy specimen. the eruption resolved after treatment with amikacin and clarithromycin. lupus vulgaris-like lesions might be included in the clinical spectrum of infections caused by rapidly growing mycobacteria.200212100204
mycobacterium tuberculosis and mycobacterium fortuitum osteomyelitis of the foot and septic arthritis of the ankle in an immunocompetent patient.mycobacteria, both tuberculous and nontuberculous, are recognized as a cause of chronic bone and joint infection. however, the diagnosis of mycobacterial infection is easily missed because of the absence of systemic involvement. moreover, specific microbiologic techniques are required to detect mycobacteria in clinical specimens. infections due to uncommon pathogens such as mycobacteria are more likely to occur in the immunocompromised host. a case of septic arthritis of the ankle and osteomyeli ...200212111605
a pulsed-field gel electrophoresis study of mycobacterium fortuitum in a caribbean setting underlines high genetic diversity of the strains and excludes nosocomial outbreaks.among rapidly-growing opportunistic mycobacteria, organisms of the mycobacterium fortuitum-mycobacterium chelonae complex (m. fortuitum, m. chelonae, m. abscessus and m. peregrinum) were isolated in significantly higher numbers during the period 1993-99 from clinical samples in guadeloupe, martinique and french guiana. based on biochemical and cultural tests and pcr-restriction fragment length polymorphism (rflp) of the hsp65 gene, 51 isolates from 47 patients were unambiguously identified as m. ...200212139429
clinical and laboratory features of mycobacterium mageritense.six clinical isolates of the nonpigmented, rapidly growing species mycobacterium mageritense were recovered from sputum, bronchial wash, blood, sinus drainage, and two surgical wound infections from separate patients in texas, new york, louisiana, and florida. the isolates matched the atcc type strain by pcr restriction enzyme analysis of the 65-kda hsp gene sequence of telenti, high-performance liquid chromatography, biochemical reactions, and partial 16s rrna gene sequencing. these are the fir ...200212149354
[effect of some biocides on non-tuberculous mycobacteria].the incidence of disease and nosocomial infections produced by non tuberculosis mycobacteria (ntm) has increased in immunocompetent patients, but also and more frequently, in immunosuppressed patients. several studies have disclosed that mycobacteria are more resistant to biocides than non-sporulating bacteria; in addition, some species are particularly resistant. the biocide action of sodium hypochloride and glutaraldehyde on mycobacterium aviumintracellulare, mycobacterium gordonae, mycobacter ...200212152479
ventriculitis following a neurosurgic procedure. 200212166797
conservative treatment of prosthetic valve endocarditis due to mycobacterium fortuitum.described here is the case of a patient with infective endocarditis in a prosthetic valve due to a mycobacterium fortuitum-group organism. the patient was treated medically and had a favorable clinical response. this is only the second report of survival after mycobacterium fortuitum-group endocarditis, and the first of survival without surgical intervention. the duration of treatment is not well defined for this patient, but life-long suppressive therapy will likely be required.200212172746
atypical mycobacterial infection following blepharoplasty and full-face skin resurfacing with co2 laser.with the popularity of laser skin resurfacing for the correction of photoaging and the improvement of acne scarring, it is important to note complications that may result from this procedure. infections must be recognized and treated early, as some can result in permanent local destruction and systemic spread to other body sites.200212174077
rapidly growing mycobacterial pulmonary infection in association with severe gastroesophageal reflux disease.pulmonary infection due to rapidly growing mycobacteria (runyan group iv) is uncommon and may be overlooked or misdiagnosed. esophageal disorders have been recognized as a potential risk factor predisposing for this infection. a 35-year-old sri lankan patient, with severe gastroesophageal reflux disease and a hiatus hernia, contracted a pulmonary infection with mycobacterium fortuitum-chelonae. he had severe airway obstruction and focal bronchiectasis, and responded to treatment with ciprofloxac ...200212174240
levofloxacin alone efficiently treated a cutaneous mycobacterium fortuitum infection.cutaneous infections with mycobacterium (m.) fortuitum are rare, and some cases resist treatment. we report two cases of cutaneous m. fortuitum infection successfully treated with levofloxacin alone.200212184647
virulent mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion.the virulence of different isolates of mycobacterium has been associated with two morphologically distinguishable colonial variants: opaque (smop) and transparent (smtr). in this report we used an in vitro assay to compare macrophage (mphi) responses to smop and smtr mycobacterium fortuitum variants, taking advantage of the fact that these variants were derived from the same isolate. cells preactivated or not with gamma interferon (ifn-gamma) were infected with smop or smtr m. fortuitum. we show ...200212228291
comparison of the in vitro activity of the glycylcycline tigecycline (formerly gar-936) with those of tetracycline, minocycline, and doxycycline against isolates of nontuberculous mycobacteria.we compared the in vitro activity of the glycylcycline tigecycline (formerly gar-936) with those of tetracycline, doxycycline, and minocycline by broth microdilution against 76 isolates belonging to seven species of rapidly growing mycobacteria (rgm) and 45 isolates belonging to five species of slowly growing nontuberculous mycobacteria (ntm). by using a resistance breakpoint of >4 micro g/ml for tigecycline and >8 micro g/ml for tetracycline, all rgm were highly susceptible to tigecycline, with ...200212234839
Displaying items 601 - 700 of 1328