| insights from genomes of representatives of the human gut commensal bifidobacterium bifidum. | bifidobacteria are bacterial gut commensals of mammals, birds and social insects that are perceived to influence the metabolism/physiology of their host. in this context, members of the bifidobacterium bifidum species are believed to significantly contribute to the overall microbiota of the human gut at infant stage. however, the molecular reasons for their adaptation to this environment are poorly understood. in this study, we analysed the pan-genome of b. bifidum species by decoding genomes of ... | 2015 | 25523018 |
| effect of probiotic supplementation on bacterial translocation in common bile duct obstruction. | to investigate the effects of probiotics on bacterial translocation in the obstructive common bile duct with comparison to an enteral product containing arginine and glutamine. | 2015 | 25503581 |
| performance evaluation of bulk freeze dried starter cultures of dahi and yoghurt along with probiotic strains in standardized milk of cow and buffalo. | performance of bulk freeze dried (bfd) cultures of dahi (d) and yoghurt (y) either with or without probiotic cultures (ab -lactobacillus acidophilus and bifidobacterium bifidum) in standardized milk of cow and buffalo was evaluated. in buffalo milk, significantly (p < 0.05) low viable count of probiotic culture combination of dahi cultures (dab) over non probiotic combination (d) was noticed; whereas, difference in counts of yoghurt culture combinations y and yab was not significant. the culture ... | 2014 | 25477690 |
| bifidobacterium bifidum in probiotic edam cheese: influence on cheese ripening. | a study was conducted to determine the efficacy of edam cheese as a carrier of probiotic bifidobacteria. probiotic edam cheese containing 10(7) viable cells of bifidobacterium bifidum was monitored over a period of 3 months for ripening changes. lactose in control as well as experimental cheeses was depleted within 15 days. moisture decreased from 46.61 and 47.24 % to 42.06 and 42.46 % in control and experimental samples, respectively while ph in both the cheeses increased from ~5.20 to ~5.45. t ... | 2014 | 25477659 |
| probiotic administration modifies the milk fatty acid profile, intestinal morphology, and intestinal fatty acid profile of goats. | the effect of a mixture of potentially probiotic bacteria (mppb; lactobacillus reuteri ddl 19, lactobacillus alimentarius ddl 48, enterococcus faecium dde 39, and bifidobacterium bifidum strains) on the milk fatty acid (fa) profile, with emphasis on cis-9,trans-11 conjugated linoleic acid (cla) in the middle stage of goat lactation, was determined. in addition, the effects of mppb feeding on the fa profile in intestinal content and intestinal morphology in weaned goats were analyzed. the probiot ... | 2015 | 25465559 |
| effect of oral probiotics supplementation in the prevention of necrotizing enterocolitis among very low birth weight preterm infants. | necrotizing enterocolitis (nec) is the most serious gastrointestinal problem in very low birth weight preterm infants. multiple risk factors activate the inflammatory cascade leading to high expressions of pro-inflammatory mediators causing bowel injury in nec. the anti-inflammatory effect of probiotics is due to the inhibition and reduction of inflammatory signal in intestinal epithelium. | 2014 | 25391168 |
| α-n-acetylglucosaminidase from bifidobacterium bifidum specifically hydrolyzes α-linked n-acetylglucosamine at nonreducing terminus of o-glycan on gastric mucin. | α-linked n-acetylglucosamine is one of the major glyco-epitopes in o-glycan of gastroduodenal mucin. here, we identified glycoside hydrolase (gh) family 89 α-n-acetylglucosaminidase, termed agnb, from bifidobacterium bifidum jcm 1254, which is essentially specific to glcnacα1-4gal structure. agnb is a membrane-anchored extracellular enzyme consisting of a gh89 domain and four carbohydrate-binding module (cbm) 32 domains. among four cbm32 domains, three tandem ones at c-terminus showed to bind po ... | 2015 | 25381911 |
| high expression of β-glucosidase in bifidobacterium bifidum bgn4 and application in conversion of isoflavone glucosides during fermentation of soy milk. | in spite of the reported probiotic effects, bifidobacterium bifidum bgn4 (bgn4) showed no β- glucosidase activity and failed to biotransform isoflavone glucosides into the more bioactive aglycones during soy milk fermentation. to develop an isoflavone-biotransforming bgn4, we constructed the recombinant b. bifidum bgn4 strain (b919g) by cloning the structural β- glucosidase gene from b. lactis ad011 (ad011) using the expression vector with the constitutively active promoter 919 from bgn4. as a r ... | 2015 | 25348696 |
| microencapsulation of probiotics by calcium alginate-gelatinized starch with chitosan coating and evaluation of survival in simulated human gastro-intestinal condition. | microencapsulation as one of the most modern methods has considerable effects on probiotic survival. in this study lactobacillus casei (atcc 39392) and bifidobacterium bifidum (atcc 29521) were encapsulated using calcium alginate-gelatinized starch, chitosan coating and inulin via emulsion technique, and were incubated in simulated gastric juice (along with pepsin, ph=1.5) and simulated intestinal juice (along with pancreatin and bile salts, ph = 8) for 2 hours at 37 (o)c. the morphology and siz ... | 2014 | 25276184 |
| short-term probiotic therapy alleviates small intestinal bacterial overgrowth, but does not improve intestinal permeability in chronic liver disease. | : although numerous animal studies suggest that probiotic therapy has beneficial effects in various liver diseases, the evidence for beneficial effects in human liver disease is controversial. this study was carried out to investigate the efficacy of probiotic therapy in alleviating small intestinal bacterial overgrowth (sibo) and permeability in chronic liver disease. | 2014 | 25244414 |
| bifidobacterium bifidum as an example of a specialized human gut commensal. | bifidobacteria are considered dominant and for this reason key members of the human gut microbiota, particularly during the first one to two years following birth. a substantial proportion of the bifidobacterial population in the intestine of infants belong to the bifidobacterium bifidum taxon, whose members have been shown to display remarkable physiological and genetic features involving adhesion to epithelia, as well as utilization of host-derived glycans. here, we reviewed the current knowle ... | 2014 | 25191315 |
| probiotic bifidobacterium species stimulate human slc26a3 gene function and expression in intestinal epithelial cells. | slc26a3, or downregulated in adenoma (dra), plays a major role in mediating cl(-) absorption in the mammalian intestine. disturbances in dra function and expression have been implicated in intestinal disorders such as congenital cl(-) diarrhea and gut inflammation. we previously showed that an increase in dra function and expression by lactobacillus acidophilus and its culture supernatant (cs) might underlie antidiarrheal effects of this probiotic strain. however, the effects of bifidobacterium ... | 2014 | 25143346 |
| a multispecies probiotic reduces oral candida colonization in denture wearers. | the prevalence of candida infections has been rising with an increasingly aging population and a larger population of immunocompromised individuals. the use of probiotics may be an alternative approach to antifungal agents in the prevention and treatment of oral candidiasis. this study aimed to evaluate the short-term effect of probiotics in reducing the infection level of oral candida in candidiasis-asymptomatic elderly denture wearers. | 2015 | 25143068 |
| isolation and molecular identification of lactic acid bacteria and bifidobacterium spp. from faeces of the blue-fronted amazon parrot in brazil. | in brazil, the blue-fronted amazon parrot (amazona aestiva) is a common pet. the faecal microbiota of these birds include a wide variety of bacterial species, the majority of which belong to the gram-positive lactic acid bacteria (lab) clade. the aim of this study was to investigate differences in the diversity and abundance of lab and bifidobacterium spp. in the cloacae between wild and captive birds and to select, identify and characterise lab for consideration as a parrot probiotic. cloacal s ... | 2014 | 25062609 |
| phasic study of intestinal homeostasis disruption in experimental intestinal obstruction. | to investigate the phasic alteration of intestinal homeostasis in an experimental model of intestinal obstruction. | 2014 | 25009385 |
| expression of sortase-dependent pili of bifidobacterium bifidum prl2010 in response to environmental gut conditions. | cell surface pili have recently been found in many different bifidobacterial species, including the infant gut commensal bifidobacterium bifidum prl2010. pili produced by prl2010 have been shown to be important molecular mediators for bacterial interaction with its human host. however, nothing is known about the modulation of their expression in response to cues that reflect the gastro intestinal environment, such as thermal, acidic, and osmotic challenges, or the presence of other gut microorga ... | 2014 | 24947069 |
| influence of bovine lactoferrin on the growth of selected probiotic bacteria under aerobic conditions. | bovine lactoferrin (blf) is a natural glycoprotein, and it shows broad-spectrum antimicrobial activity. however, reports on the influences of blf on probiotic bacteria have been mixed. we examined the effects of apo-blf (between 0.25 and 128 mg/ml) on both aerobic and anaerobic cultures of probiotics. we found that blf had similar effects on the growth of probiotics under aerobic or anaerobic conditions, and that it actively and significantly (at concentrations of >0.25 mg/ml) retarded the growt ... | 2014 | 24916115 |
| dahi containing lactobacillus acidophilus and bifidobacterium bifidum improves phagocytic potential of macrophages in aged mice. | the present study investigated the effect of dahi containing potential probiotic bacterial strains on macrophage functions in ageing mice. probiotic dahi was prepared by co-culturing dahi bacteria (lactococcus lactis ssp. cremoris and lactococcus lactis ssp. lactis biovar diacetylactis) along with lactobacillus acidophilus lavk2 (la-dahi) or combined l. acidophilus lavk2 and bifidobacterium bifidum bbvk3 (labb-dahi) in buffalo milk. the effect of ageing on phagocytic function was evaluated on 4 ... | 2014 | 24876648 |
| murein lytic enzyme tgaa of bifidobacterium bifidum mimbb75 modulates dendritic cell maturation through its cysteine- and histidine-dependent amidohydrolase/peptidase (chap) amidase domain. | bifidobacteria are gram-positive inhabitants of the human gastrointestinal tract that have evolved close interaction with their host and especially with the host's immune system. the molecular mechanisms underlying such interactions, however, are largely unidentified. in this study, we investigated the immunomodulatory potential of bifidobacterium bifidum mimbb75, a bacterium of human intestinal origin commercially used as a probiotic. particularly, we focused our attention on tgaa, a protein ex ... | 2014 | 24814791 |
| metabolism of sialic acid by bifidobacterium breve ucc2003. | bifidobacteria constitute a specific group of commensal bacteria that inhabit the gastrointestinal tracts of humans and other mammals. bifidobacterium breve ucc2003 has previously been shown to utilize several plant-derived carbohydrates that include cellodextrins, starch, and galactan. in the present study, we investigated the ability of this strain to utilize the mucin- and human milk oligosaccharide (hmo)-derived carbohydrate sialic acid. using a combination of transcriptomic and functional g ... | 2014 | 24814790 |
| potential of goat probiotic to bind mutagens. | the mutagen binding ability of the goat probiotics (lactobacillus reuteri ddl 19, lactobacillus alimentarius ddl 48, enterococcus faecium dde 39, and bifidobacterium bifidum ddba) was evaluated. the oral administration of these probiotics reduced fecal mutagens and intestinal cancer markers in goats. secondly, the effects of probiotics against the mutagenesis induced by sodium azide (sa), and benzopyrene (b[α]p) by performing the modified ames test using salmonella typhimurium ta 100 was investi ... | 2014 | 24785349 |
| lactoferrin and bifidobacteria. | we herein summarized the effects of lactoferrin (lf) on bifidobacteria. many in vitro studies previously reported the growth-promoting (bifidogenic) effects of lf on bifidobacteria. the involvement of bound iron, sugar chains, and lf peptides has been proposed in this bifidogenic mechanism. peptides in the lf pepsin hydrolysate (lfh) showed stronger bifidogenic activity than natural lf; therefore, we speculated that peptides may be the bifidogenic active principle of lf. lf or its peptides may b ... | 2014 | 24770988 |
| comparative determination of two probiotics by qcm and owls-based immunosensors. | the regular consumption of foods containing probiotic bacteria has beneficial physiological effects on the health and the digestion system. there is a need for novel analytical approaches for the determination of these bacteria that are faster than the classical plate counting method. for this purpose, two label-free biosensors were investigated and presented in this paper: quartz crystal microbalance (qcm) and optical waveguide lightmode spectroscopy (owls) based direct immunosensors were devel ... | 2014 | 24768869 |
| kefir fermented milk and kefiran promote growth of bifidobacterium bifidum prl2010 and modulate its gene expression. | bifidobacteria constitute one of the dominant groups of microorganisms colonizing the human gut of infants. their ability to utilize various host-derived glycans as well as dietary carbohydrates has received considerable scientific attention. however, very little is known about the role of fermented foods, such as kefir, or their constituent glycans, such as kefiran, as substrates for bifidobacterial growth and for the modulation of the expression of bifidobacterial host-effector molecules. here ... | 2014 | 24667318 |
| bifidobacterium and enteral feeding in preterm infants: cluster-randomized trial. | this study evaluated the benefit of bifidobacterium bifidum olb6378 (b. bifidum) in very low-birthweight (vlbw) infants (birthweight <1500 g) for the acceleration of enteral feeding. | 2014 | 24617812 |
| [application of multiplex pcr for analysis of polycomponent probiotic preparations]. | development of multiplex pcr for indication and differential analysis of cfu in a probiotic preparation containing 3 various species ofbifidobacteria (bifidobacterium bifidum, b. longum, b. adolescentis) and 2 species of lactobacilli (lactobacillus acidophilus, l. plantarum). materials and methods; multiplex pcr with primers to 16s rna genes of 5 species of bacteria was applied. samples of bacterial colonies were removed from solid nutrient medium and transferred into test-tube for multiplex pcr ... | 2014 | 24605661 |
| expression of fluorescent proteins in bifidobacteria for analysis of host-microbe interactions. | bifidobacteria are an important component of the human gastrointestinal microbiota and are frequently used as probiotics. the genetic inaccessibility and lack of molecular tools commonly used in other bacteria have hampered a detailed analysis of the genetic determinants of bifidobacteria involved in their adaptation to, colonization of, and interaction with the host. in the present study, a range of molecular tools were developed that will allow the closing of some of the gaps in functional ana ... | 2014 | 24584243 |
| probiotic dahi containing lactobacillus acidophilus and bifidobacterium bifidum modulates the formation of aberrant crypt foci, mucin-depleted foci, and cell proliferation on 1,2-dimethylhydrazine-induced colorectal carcinogenesis in wistar rats. | aberrant crypt foci (acf) and mucin-depleted foci (mdf) are pre-neoplastic lesions identified in the colon of carcinogen-treated rodents and in humans at high risk for colon cancer. the present study was carried out to divulge the protective potential of the probiotic dahi containing lactobacillus acidophilus lavk2 and bifidobacterium bifidum bbvk3 alone or in combination with piroxicam (pxc) on the development of early biomarkers of colorectal carcinogenesis in male wistar rats administered 1,2 ... | 2014 | 24524423 |
| effect of denak (oliveria decumbens vent) on growth and survival of lactobacillus acidophilus and bifidobacterium bifidum for production of probiotic herbal milk and yoghurt. | this study was undertaken to determine the suitability of different doses (0, 0.5, 1.0 and 1.5%) of denak powder (oliveria decumbens vent) on viability of lactobacillus acidophilus and bifidobacterium bifidum in milk and yoghurt during 21 day refrigerated storage for production of probiotic herbal milk and yoghurt. in order to determine the effect of different doses of denak powder on growth of probiotic bacteria in milk and yoghurt, first lyophilized bacteria lactobacillus acidophilus was added ... | 2013 | 24517020 |
| cost-benefit analysis of the probiotic treatment of children hospitalized for acute diarrhea in bangkok, thailand. | we studied the cost-benefit of using probiotics (lactobacillus acidophilus and bifidobacterium bifidum) in the treatment of 106 children hospitalized with acute diarrhea using a double-blind randomized, placebo-controlled trial. the median length of hospital stay was significantly shorter in the probiotics group than in the controlled group (2 versus 3 days, p=0.049), but the median duration of diarrhea and direct medical costs were not significantly different (4 versus 5 days, p=0.068 and 4,418 ... | 2013 | 24450244 |
| lactobacillus strains isolated from infant faeces possess potent inhibitory activity against intestinal alpha- and beta-glucosidases suggesting anti-diabetic potential. | inhibitors of intestinal alpha-glucosidases are used therapeutically to treat type 2 diabetes mellitus. bacteria such as actinoplanes sp. naturally produce potent alpha-glucosidase inhibitor compounds, including the most widely available drug acarbose. it is not known whether lactic acid bacteria (lab) colonising the human gut possess inhibitory potential against glucosidases. hence, the study was undertaken to screen labs having inherent alpha- and beta-glucosidase inhibitory potential. | 2014 | 24414142 |
| identification and characterization of the serine/threonine protein kinases in bifidobacterium. | six genes encoding the bifidobacterial hanks-type (eukaryote-like) serine/threonine protein kinases (stpk) were identified and classified. the genome of each bifidobacterial strain contains four conserved genes and one species-specific gene. bifidobacterium longum and bifidobacterium bifidum possess the unique gene found only in these species. the stpk genes of russian industrial probiotic strain b. longum b379m were cloned and sequenced. the expression of these genes in escherichia coli and bif ... | 2014 | 24395073 |
| multistrain probiotic modulation of intestinal epithelial cells' immune response to a double-stranded rna ligand, poly(i·c). | a commercially available product containing three probiotic bacterial strains (lactobacillus helveticus r0052, bifidobacterium longum subsp. infantis r0033, and bifidobacterium bifidum r0071) was previously shown in animal trials to modulate both th1 and th2 immune responses. clinical studies on this combination of bacteria have also shown positive health effects against seasonal winter diseases and rotavirus infection. the goal of this study was to use a well-established in vitro intestinal epi ... | 2014 | 24375132 |
| polysaccharide from seeds of plantago asiatica l. affects lipid metabolism and colon microbiota of mouse. | polysaccharide from the seeds of plantago asiatica l. was given via oral administration to mice (0.4 g/kg body weight, 30 days) to observe its effects on mouse nutrient metabolism and colon microbiota. it was found the polysaccharide intake could lower the apparent absorption of lipid. total triglyceride, cholesterol, and atherogenic index in blood serum with total lipid and cholesterol levels in liver of polysaccharide group mice were all significantly lower than those of the control group (p < ... | 2014 | 24341731 |
| isolation and structural identification of glycopolymers of bifidobacterium bifidum bim b-733d as putative players in pathogenesis of autoimmune thyroid diseases. | bifidobacterium bifidum 791 (commercially available as b. bifidum bim b-733d) cell-surface biopolymers (bps) interact selectively with human serum thyroid peroxidase (tpo) and thyroglobulin (tg) autoantibodies (anti tpo and anti tg, respectively). bpanti-tpo and bpanti-tg were isolated from the soluble fraction of b. bifidum bim b-733d by affinity chromatography with anti-tpo or anti-tg, respectively. homogeneity of affinity eluates (aeanti-tpo and aeanti-tg) was tested by size exclusion chromat ... | 2013 | 24311320 |
| probiotic supplement consumption alters cytokine production from peripheral blood mononuclear cells: a preliminary study using healthy individuals. | the objective of this study was to examine the effect of daily probiotic supplementation upon the immune profile of healthy participants by the assessment of ex vivo cytokine production. twenty healthy adult volunteers received a multi-strain probiotic supplement consisting of two strains of lactobacillus acidophilus (cul60 and cul21), bifidobacterium lactis (cul34) and bifidobacterium bifidum (cul20) and fructooligosaccharide for 12 weeks. blood samples were collected at baseline, 6 and 12 week ... | 2013 | 24311314 |
| bifidobacterium bifidum prl2010 modulates the host innate immune response. | here, we describe data obtained from transcriptome profiling of human cell lines and intestinal cells of a murine model upon exposure and colonization, respectively, with bifidobacterium bifidum prl2010. significant changes were detected in the transcription of genes that are known to be involved in innate immunity. furthermore, results from enzyme-linked immunosorbent assays (elisas) showed that exposure to b. bifidum prl2010 causes enhanced production of interleukin 6 (il-6) and il-8 cytokines ... | 2014 | 24242237 |
| effect of lactobacillus acidophilus and bifidobacterium bifidum supplementation to standard triple therapy on helicobacter pylori eradication and dynamic changes in intestinal flora. | to investigate lactobacillus acidophilus (l. acidophilus) and bifidobacterium bifidum (b. bifidum) supplementation to triple therapy for helicobacter pylori (h. pylori) eradication and dynamic changes in intestinal flora in children with h. pylori infection. one hundred h. pylori-infected children were randomly assigned to two groups: treatment group (n = 43), standard triple anti-h. pylori therapy plus probiotics of l. acidophilus and b. bifidum for 2 weeks followed by taking probiotics for ano ... | 2014 | 24233772 |
| novel probiotic-fermented milk with angiotensin i-converting enzyme inhibitory peptides produced by bifidobacterium bifidum mf 20/5. | in previous research, we have demonstrated that bifidobacterium bifidum mf 20/5 fermented milk possessed stronger angiotensin converting enzyme (ace) inhibitory activity than other lactic acid bacteria, including lactobacillus helveticus dsm 13137, which produces the hypotensive casokinins ile-pro-pro (ipp) and val-pro-pro (vpp). the aim of this study is to investigate the ace-inhibitory peptides released in b. bifidum mf 20/5 fermented milk. the novel ace-inhibitory peptide lvypfp (ic50 = 132 μ ... | 2013 | 24135669 |
| role of probiotics in the prevention and treatment of meticillin-resistant staphylococcus aureus infections. | meticillin-resistant staphylococcus aureus (mrsa) is a multidrug-resistant micro-organism and is the principal nosocomial pathogen worldwide. following initial in vitro experiments demonstrating that lactobacillus acidophilus cl1285(®) and lactobacillus casei lbc80r(®) commercial strains exhibit antibacterial activity against clinical mrsa isolates, we conducted a literature search to find any evidence of probiotic efficacy in decolonisation or treatment of s. aureus infection. as summarised bel ... | 2013 | 24071026 |
| bopa does not have a major role in the adhesion of bifidobacterium bifidum to intestinal epithelial cells, extracellular matrix proteins, and mucus. | the ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. bifidobacterium bifidum strains dsm20456 and mimbb75 adhere well to the human intestinal cell lines caco-2 and ht-29. the surface lipoprotein bopa was previously described to be involved in mediating adherence of b. bifidum to epithelial cells, but thioacylated, purified bopa inhibited the adhesion of b. bifidum to epithelial cells i ... | 2013 | 24014530 |
| unraveling the leloir pathway of bifidobacterium bifidum: significance of the uridylyltransferases. | the gnb/lnb (galacto-n-biose/lacto-n-biose) pathway plays a crucial role in bifidobacteria during growth on human milk or mucin from epithelial cells. it is thought to be the major route for galactose utilization in bifidobacterium longum as it is an energy-saving variant of the leloir pathway. both pathways are present in b. bifidum, and galactose 1-phosphate (gal1p) is considered to play a key role. due to its toxic nature, gal1p is further converted into its activated udp-sugar through the ac ... | 2013 | 24014529 |
| probiotic use and prevalence of candidemia and candiduria in a picu. | to compare the prevalence of candidemia and candiduria before and after the introduction of routine use of probiotics in children who received broad-spectrum antibiotics in a picu. | 2013 | 23965638 |
| "amide resonance" in the catalysis of 1,2-α-l-fucosidase from bifidobacterium bifidum. | bifidobacterium is a genus of gram-positive bacteria, which is important in the absorption of nourishment from the human milk oligosaccharides (hmo). we present here the detailed simulation of the enzymatic hydrolysis of 2'-fucosyllactose catalyzed by 1,2-α-l-fucosidase from bifidobacterium bifidum using the combined quantum mechanical and molecular mechanical approach. molecular dynamics simulations and free energy profiles support that the overall reaction is a stepwise mechanism. the first st ... | 2013 | 23952813 |
| variation in consumption of human milk oligosaccharides by infant gut-associated strains of bifidobacterium breve. | human milk contains a high concentration of complex oligosaccharides that influence the composition of the intestinal microbiota in breast-fed infants. previous studies have indicated that select species such as bifidobacterium longum subsp. infantis and bifidobacterium bifidum can utilize human milk oligosaccharides (hmo) in vitro as the sole carbon source, while the relatively few b. longum subsp. longum and bifidobacterium breve isolates tested appear less adapted to these substrates. conside ... | 2013 | 23892749 |
| probiotics for the prevention of necrotizing enterocolitis in neonates: an 8-year retrospective cohort study. | probiotic therapy has been shown to reduce morbidity and mortality of necrotizing enterocolitis (nec) in premature infants in several international studies using various probiotic agents. the purpose of this study(*) is to describe our experience of using probiotic therapy in preventing nec in infants with very low birth weight (vlbw) in a neonatal intensive care unit (nicu) and to evaluate whether our records provide evidence of effectiveness for probiotic therapy. | 2013 | 23865733 |
| lacto-n-biosidase encoded by a novel gene of bifidobacterium longum subspecies longum shows unique substrate specificity and requires a designated chaperone for its active expression. | infant gut-associated bifidobacteria possess species-specific enzymatic sets to assimilate human milk oligosaccharides, and lacto-n-biosidase (lnbase) is a key enzyme that degrades lacto-n-tetraose (galβ1-3glcnacβ1-3galβ1-4glc), the main component of human milk oligosaccharides, to lacto-n-biose i (galβ1-3glcnac) and lactose. we have previously identified lnbase activity in bifidobacterium bifidum and some strains of bifidobacterium longum subsp. longum (b. longum). subsequently, we isolated a g ... | 2013 | 23843461 |
| [dynamic changes of immune responses in balb/c mice immunized with a recombinant bb(pgex-sj14-3-3) vaccine of schistosoma japonicum]. | to investigate the dynamic changes of the immune responses in mice immunized with a recombinant (bifidobacterium bifidum, bb) (pgex-sj14-3-3) vaccine of schistosoma japonicum. | 2013 | 23837975 |
| role of sortase-dependent pili of bifidobacterium bifidum prl2010 in modulating bacterium-host interactions. | bifidobacteria represent one of the dominant groups of microorganisms colonizing the human infant intestine. commensal bacteria that interact with a eukaryotic host are believed to express adhesive molecules on their cell surface that bind to specific host cell receptors or soluble macromolecules. whole-genome transcription profiling of bifidobacterium bifidum prl2010, a strain isolated from infant stool, revealed a small number of commonly expressed extracellular proteins, among which were gene ... | 2013 | 23776216 |
| effect of water-soluble fraction of cherry tomatoes on the adhesion of probiotics and salmonella to intestinal epithelial cells. | tomato is one of the most consumed vegetables in the world and contains many valuable nutritional components. here we investigate the prebiotic effects of cherry tomatoes for improving gut health. | 2013 | 23749725 |
| protective effect of bifidobacteria in an experimental model of clostridium difficile associated colitis. | the aim of this study was to evaluate the ability of bifidobacterium strains to prevent the effects associated with clostridium difficile infection in a hamster model of enterocolitis. after clindamycin treatment (30 mg/kg), animals were infected intragastrically with c. difficile (5×108 cfu per animal). seven days prior to antibiotic administration, probiotic treatment was started by administering bacterial suspensions of bifidobacteria in drinking water. strains cidca 531, cidca 5310, cidca 53 ... | 2013 | 23611644 |
| impact of bifidobacterium bifidum mimbb75 on mouse intestinal microorganisms. | bifidobacterium bifidum mimbb75 is a recently identified probiotic. however, its distribution along the intestine and impact on resident microbiota is unknown. herein, we established a quantitative real-time polymerase chain reaction assay targeting the b. bifidum-specific bopa region for the quantification of b. bifidum in feces and used this assay to investigate transit of b. bifidum mimbb75 through the murine intestine. we also analyzed the consequential impact on resident microbial cohorts. ... | 2013 | 23551062 |
| efficacy of different probiotic combinations on death and necrotizing enterocolitis in a premature rat model. | the aim of the present study was to investigate the most effective probiotic combinations to prevent death and necrotizing enterocolitis (nec) in a premature rat model. | 2013 | 23535766 |
| association of a probiotic to a helicobacter pylori eradication regimen does not increase efficacy or decreases the adverse effects of the treatment: a prospective, randomized, double-blind, placebo-controlled study. | the treatment for the eradication of helicobacter pylori (h. pylori) is complex; full effectiveness is rarely achieved and it has many adverse effects. in developing countries, increased resistance to antibiotics and its cost make eradication more difficult. probiotics can reduce adverse effects and improve the infection treatment efficacy.if the first-line therapy fails a second-line treatment using tetracycline, furazolidone and proton-pump inhibitors has been effective and low cost in brazil; ... | 2013 | 23530767 |
| evaluation of adhesion properties and antibacterial activities of the infant gut commensal bifidobacterium bifidum prl2010. | bifidobacteria are extensively exploited by the food industry as health-promoting microorganisms. however, very little is known about the molecular mechanisms responsible for these beneficial activities, or the molecular players that sustain their ability to colonize and persist within the human gut. here, we have investigated the enteric adaptation features of the gut commensal bifidobacterium bifidum prl2010, originally isolated from infant feces. this strain was able to survive under gastroin ... | 2013 | 23523946 |
| crystal structures of a glycoside hydrolase family 20 lacto-n-biosidase from bifidobacterium bifidum. | human milk oligosaccharides contain a large variety of oligosaccharides, of which lacto-n-biose i (gal-β1,3-glcnac; lnb) predominates as a major core structure. a unique metabolic pathway specific for lnb has recently been identified in the human commensal bifidobacteria. several strains of infant gut-associated bifidobacteria possess lacto-n-biosidase, a membrane-anchored extracellular enzyme, that liberates lnb from the nonreducing end of human milk oligosaccharides and plays a key role in the ... | 2013 | 23479733 |
| strain-specific identification of bifidobacterium bifidum olb6378 by pcr. | the aim of the present study was to develop a strain-specific polymerase chain reaction (pcr) primer set for the detection of bifidobacterium bifidum olb6378 (olb6378) that can serve as suitable probiotics for infants. the random amplified polymorphic dna (rapd)-pcr technique was used to obtain olb6378-specific pcr products. one olb6378-specific rapd-pcr product was obtained after testing 97 rapd primers, and was sequenced. thirteen pcr primer sets were designed from the sequence. one pcr primer ... | 2013 | 23470759 |
| a way to follow the viability of encapsulated bifidobacterium bifidum subjected to a freeze-drying process in order to target the colon: interest of flow cytometry. | the aim of this work was to apply flow cytometry in order to assess and compare the viability of freeze-dried entrapped bacteria with an usual technique by quantification by plate count techniques. it also aimed at studying the effect of various cryoprotectants on the viability of an entrapped bifidobacterium bifidum subjected to freeze-drying to check their ability to be delivered all along the gastro-intestinal tract. the alginate-pectinate beads were chosen as the encapsulation matrix added w ... | 2013 | 23459108 |
| comparison of the compositions of the stool microbiotas of infants fed goat milk formula, cow milk-based formula, or breast milk. | the aim of the study was to compare the compositions of the fecal microbiotas of infants fed goat milk formula to those of infants fed cow milk formula or breast milk as the gold standard. pyrosequencing of 16s rrna gene sequences was used in the analysis of the microbiotas in stool samples collected from 90 australian babies (30 in each group) at 2 months of age. beta-diversity analysis of total microbiota sequences and lachnospiraceae sequences revealed that they were more similar in breast mi ... | 2013 | 23455335 |
| in vitro evaluation of bifidobacterium strains of human origin for potential use in probiotic functional foods. | the present study investigated some in vitro properties for probiotic use of four strains of bifidobacteria isolated from faeces of healthy children (bifidobacterium longum 51a, bifidobacterium breve 1101a, bifidobacterium pseudolongum 1191a and bifidobacterium bifidum 1622a). in vitro tests were carried out to compare growth rate, aerotolerance, antagonistic activity against pathogens, antimicrobial susceptibility profile and cell wall hydrophobicity. mean doubling time of b. longum 51a was sho ... | 2013 | 23443950 |
| effect of microencapsulation methods on the survival of freeze-dried bifidobacterium bifidum. | six kinds of bifidobacterium bifidum microcapsules were prepared by extrusion methods, emulsion methods and coacervation methods. effects of preparation methods on the survival of encapsulated b. bifidum were examined. results showed that microcapsules prepared by emulsion method with alginate and chitosan exhibited the best protection for b. bifidum. the diameter was 10-20 µm, encapsulation efficiency was 90.36% and the live cell amount was 3.01 × 10₉ cfu/g after freeze-drying. encapsulated cel ... | 2013 | 23405847 |
| treatment of nonalcoholic steatohepatitis with probiotics. a proof-of-concept study. | probiotics have profound effect on nonalcoholic steatohepatitis (nash) in animal models. we aimed to test the hypothesis that probiotics treatment was superior to usual care in reducing liver fat in nash patients. | 2015 | 23396737 |
| probiotics lactobacillus rhamnosus gg, lactobacillus acidophilus suppresses dmh-induced procarcinogenic fecal enzymes and preneoplastic aberrant crypt foci in early colon carcinogenesis in sprague dawley rats. | diet makes an important contribution to colorectal cancer (crc) risk implying risks for crc are potentially reducible. therefore, the probiotics have been suggested as the prophylactic measure in colon cancer. in this study, different probiotics were used to compare their protective potential against 1,2 dimethylhydrazine dihydrochloride (dmh)-induced chemical colon carcinogenesis in sprague dawley rats. animals belonging to different probiotic groups were fed orally with 1 × 10(9) lactobacilli ... | 2013 | 23368917 |
| evaluation of efficacy of probiotics in prevention of candida colonization in a picu-a randomized controlled trial. | to evaluate the efficacy of probiotics in prevention of candida colonization in a picu. | 2013 | 23361033 |
| quantitative detection of viable bifidobacterium bifidum bf-1 cells in human feces by using propidium monoazide and strain-specific primers. | we developed a pcr-based method to detect and quantify viable bifidobacterium bifidum bf-1 cells in human feces. this method (pma-qpcr) uses propidium monoazide (pma) to distinguish viable from dead cells and quantitative pcr using a bf-1-specific primer set designed from the results of randomly amplified polymorphic dna analysis. during long-term culture (10 days), the number of viable bf-1 cells detected by counting the number of cfu on modified mrs agar, by measuring the atp contents converte ... | 2013 | 23354719 |
| antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. | previous reports have shown that several probiotic strains can resist the antibacterial activity of bovine lactoferrin (blf), but the results are inconsistent. moreover, a portion of orally administered apo-blf is digested in vivo by pepsin to yield blf hydrolysate, which produces stronger antibacterial activity than that observed with apo-blf. however, whether blf hydrolysate affects the growth of probiotic strains is unclear. therefore, various probiotic strains in taiwan were collected and ev ... | 2013 | 23332852 |
| exploring the ameliorative potential of probiotic dahi containing lactobacillus acidophilus and bifidobacterium bifidum on dextran sodium sulphate induced colitis in mice. | conventional medical therapies for ulcerative colitis (uc) are still limited due to the adverse side effects like dose-dependent diarrhoea and insufficient potency to keep in remission for long-term periods. so, new alternatives that provide more effective and safe therapies for ulcerative colitis are constantly being sought. in the present study, probiotic labb dahi was selected for investigation of its therapeutic effect on dss-induced colitis model in mice. labb dahi was prepared by co-cultur ... | 2013 | 23317563 |
| the effect of a multispecies synbiotic mixture on the duration of diarrhea and length of hospital stay in children with acute diarrhea in turkey: single blinded randomized study. | probiotics have been successfully used for the treatment of acute diarrhea in children and this effect depends on the strains and dose. the aim of this study was to assess the effect of a synbiotic mixture on the duration of diarrhea and the length of hospital stay in children with acute watery diarrhea. this is a prospective randomized, multicenter single blinded clinical trial in hospitalized children with acute watery diarrhea. all children were treated with conventional hydration therapy wit ... | 2013 | 23239048 |
| hypolipidemic effects of lactic acid bacteria fermented cereal in rats. | the objectives of the present study were to investigate the efficacy of the mixed culture of lactobacillus acidophilus (dsm 20242), bifidobacterium bifidum (dsm 20082) and lactobacillus helveticus (ck60) in the fermentation of maize and the evaluation of the effect of the fermented meal on the lipid profile of rats. | 2012 | 23231860 |
| genome sequence of the immunomodulatory strain bifidobacterium bifidum lmg 13195. | in this work, we report the genome sequences of bifidobacterium bifidum strain lmg13195. results from our research group show that this strain is able to interact with human immune cells, generating functional regulatory t cells. | 2012 | 23209243 |
| effect of bifidobacterium bifidum bf-1 on gastric protection and mucin production in an acute gastric injury rat model. | homeostasis in the stomach environment is maintained by the balance of protective factors such as gastric mucus and aggressive factors such as gastric acid, stress, alcohol, and drugs. an overload of aggressive factors that upsets this balance can induce gastric injury. fermented milk that contains bifidobacterium bifidum bf-1 (bf-1), a probiotic strain, and streptococcus thermophilus yit 2021 (st) is known to improve helicobacter pylori-associated gastritis in humans. here, we investigated the ... | 2013 | 23200466 |
| microencapsulation of bifidobacterium bifidum f-35 in whey protein-based microcapsules by transglutaminase-induced gelation. | bifidobacterium bifidum f-35 was microencapsulated into whey protein microcapsules (wpms) by a transglutaminase (tgase)-induced method after optimization of gelation conditions. the performance of these wpms was compared with that produced by a spray drying method (wpms-a). wpms produced by the tgase-induced gelation method (wpms-b) had larger and denser structures in morphological examinations. native gel and sds-page analyses showed that most of the polymerization observed in wpms-b was due to ... | 2012 | 23163944 |
| assessment of intestinal microbiota modulation ability of bifidobacterium strains in in vitro fecal batch cultures from preterm neonates. | microbial colonization of the infant gut is essential for the development of the intestine and the immune system. the intestinal microbiota of full-term breast-fed infants is considered as the health standard for newborns. a culture medium containing formula milk was designed, which allowed a balanced growth of intestinal microorganisms and was used to perform fecal batch cultures from preterm babies. sixteen bifidobacterium strains and fructooligosaccharides (fos) were tested for their ability ... | 2013 | 23154045 |
| new screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the biacore assay. | to determine the relationship between adhesive ability of probiotics and acidic residues in human colonic mucin, we developed a new screening method using biacore to evaluate adherence of bacteria before and after sialic acid or sulphate residues were blocked or removed from mucin. | 2013 | 23136989 |
| breast milk jaundice: effect of bacteria present in breast milk and infant feces. | breast milk is an important source of bacteria in establishing the infantile intestinal microbiota that appear to influence the enterohepatic circulation of bilirubin. the aim of the present study was to evaluate the effect of breast milk's microbiological content on the development of breast milk jaundice (bmj). | 2013 | 23132163 |
| nutraceuticals of anti-inflammatory activity as complementary therapy for rheumatoid arthritis. | rheumatoid arthritis (ra) is a chronic inflammatory disease characterized by elevated oxidative stress and inflammatory biomarkers. the severe side effects of drug used during such disease necessitate the search for new and safe approaches. food is a rich source of antioxidants and anti-inflammatory bioactive constituents including phenolic compounds, polyunsaturated fatty acids, phytosterols, toccopherols, and carotenoids. we have a series of publications dealing with the anti-inflammatory acti ... | 2014 | 23104728 |
| bifidobacterial α-galactosidase with unique carbohydrate-binding module specifically acts on blood group b antigen. | bifidobacterium bifidum is one of the most frequently found bifidobacteria in the intestines of newborn infants. we previously reported that b. bifidum possesses unique metabolic pathways for o-linked glycans on gastrointestinal mucin (yoshida e, sakurama h, kiyohara m, nakajima m, kitaoka m, ashida h, hirose j, katayama t, yamamoto k, kumagai h. 2012. bifidobacterium longum subsp. infantis uses two different β-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharide ... | 2013 | 23089618 |
| mucosal immunology and probiotics. | the cross-talk between the mucosa-associated immune system and microbiota is critical in mucosal tissue homeostasis as well as in protection against infectious and inflammatory diseases occurring at mucosal sites. this recent evidence has paved the way to therapeutic approaches aimed at modulating the mucosa-associated immune system using probiotics. different strains of probiotics possess the ability to finely regulate dendritic cell (dc) activation, polarizing the subsequent t cell activity to ... | 2013 | 23054627 |
| is a multispecies probiotic mixture effective in constipation during pregnancy? 'a pilot study'. | constipation during pregnancy is a common problem. nowadays only few effective interventions are published preventing or treating constipation during pregnancy. however, their use is limited due to side-effects. this uncontrolled intervention study was performed to determine if a mixture of probiotics in the treatment of constipation during pregnancy is effective. | 2012 | 23035837 |
| introducing gut low-density array (gulda): a validated approach for qpcr-based intestinal microbial community analysis. | alterations in the human gut microbiota caused, for example, by diet, functional foods, antibiotics, or occurring as a function of age are now known to be of relevance for host health. therefore, there is a strong need for methods to detect such alterations in a rapid and comprehensive manner. in the present study, we developed and validated a high-throughput real-time quantitative pcr-based analysis platform, termed 'gut low-density array' (gulda). the platform was designed for simultaneous ana ... | 2012 | 22967145 |
| [interaction of bifidobacterium bifidum with members of normal microflora in human intestine microsymbiocenosis]. | study the influence of exometabolites of b. bifidum on biological properties of bacteria that are the members of normoflora and their ability to interact with associative microsymbionts. | 2013 | 22937707 |
| novel bifidobacterium promoters selected through microarray analysis lead to constitutive high-level gene expression. | for the development of a food-grade expression system for bifidobacterium, a strong promoter leading to high-level expression of cloned gene is a prerequisite. for this purpose, a promoter screening host-vector system for bifidobacterium has been established using β-glucosidase from bifidobacterium lactis as a reporter and bifidobacterium bifidum bgn4 as a host, which is β-glucosidase negative strain. seven putative promoters showing constitutive high-level expression were selected through micro ... | 2012 | 22923113 |
| modulation of phenotypic and functional maturation of dendritic cells by intestinal bacteria and gliadin: relevance for celiac disease. | dc maturation and functions are influenced by microbial and environmental stimuli, which could contribute to immune dysfunction. here, we have investigated the role of enterobacteria (escherichia coli cbl2 and shigella cbd8) isolated from cd patients, bifidobacteria (bifidobacterium longum cect 7347 and bifidobacterium bifidum cect 7365), and gliadins on phenotypic and functional features of mddcs and in coculture with caco-2 cells. the ultimate goal of our study is to understand the roles playe ... | 2012 | 22891290 |
| [the influence of probiotic fermented milk product on colon microbiota, hematological parameters and cell immunity in rats]. | influence of probiotic fermented milk product on the intestinal microbiota, hematological parameters and immune status of the experiment in vivo at wistar rats was studied. it was shown, that entering of probiotic strains of bifidobacterium bifidum 791, bifidobacterium longum b-379m and lactobacillus acidophilus nk1 u streptococcus thermophilus in composition fermented milk products in the total quantity of 2,1 x 10(7) cfu/ sm3 in digestive tract within three weeks has a positive influence on th ... | 2012 | 22888666 |
| complete genome sequence of the probiotic bacterium bifidobacterium bifidum strain bgn4. | bifidobacterium bifidum, a common endosymbiotic inhabitant of the human gut, is considered a prominent probiotic microorganism that may promote health. we completely decrypted the 2.2-mb genome sequence of b. bifidum bgn4, a strain that had been isolated from the fecal sample of a healthy breast-fed infant, and annotated 1,835 coding sequences. | 2012 | 22887663 |
| bacteria-mediated in vivo delivery of quantum dots into solid tumor. | semiconductor nanocrystals, so-called quantum dots (qds), promise potential application in bioimaging and diagnosis in vitro and in vivo owing to their high-quality photoluminescence and excellent photostability as well as size-tunable spectra. here, we describe a biocompatible, comparatively safe bacteria-based system that can deliver qds specifically into solid tumor of living animals. in our strategy, anaerobic bacterium bifidobacterium bifidum (b. bifidum) that colonizes selectively in hypox ... | 2012 | 22885241 |
| an efficient and reproducible method for transformation of genetically recalcitrant bifidobacteria. | this study describes an efficient transformation system for the introduction of plasmid dna into bifidobacterium bifidum prl2010 and bifidobacterium asteroides prl2011, for which to the best of our knowledge no transformation data have been reported previously. the method is based on electroporation of bifidobacterial cells, which were made competent by an optimized methodology based on varying media and growth conditions. furthermore, the transformation protocol was applied in order to design a ... | 2012 | 22640171 |
| bifidobacterial enzymes involved in the metabolism of human milk oligosaccharides. | intestinal colonization of bifidobacteria is important for the health of infants. human milk oligosaccharides (hmo) have been identified as growth factors for bifidobacteria. recently, a bifidobacterial enzymatic system to metabolize hmo was identified. 1,3-β-galactosyl-n-acetylhexosamine phosphorylase (glnbp, ec 2.4.1.211), which catalyzes the reversible phosphorolysis of galacto-n-biose (gnb) (galβ1→3galnac)] and lacto-n-biose i (lnb) (galβ1→3glcnac), is a key enzyme to explain the metabolism ... | 2012 | 22585921 |
| effect of probiotics on the treatment of children with atopic dermatitis. | atopic dermatitis, a chronic recurrent disease, is frequently encountered in clinical practice. in the last 30 years, the prevalence of atopic dermatitis has rapidly increased due to industrialization. therefore, there have been attempts in recent years to find new ways of treating and preventing atopic dermatitis. | 2012 | 22577270 |
| analysis of predicted carbohydrate transport systems encoded by bifidobacterium bifidum prl2010. | the bifidobacterium bifidum prl2010 genome encodes a relatively small set of predicted carbohydrate transporters. growth experiments and transcriptome analyses of b. bifidum prl2010 revealed that carbohydrate utilization in this microorganism appears to be restricted to a relatively low number of carbohydrates. | 2012 | 22562993 |
| differences in faecal bacteria populations and faecal bacteria metabolism in healthy adults and celiac disease patients. | differences in the intestinal microbiota between children and adults with celiac disease (cd) have been reported; however, differences between healthy adults and adults with cd have not been clearly demonstrated. the aim of this study was to evaluate the differences in the intestinal microbiota between adults with cd and healthy individuals. microbial communities in faecal samples were evaluated by pcr-denaturing gradient gel electrophoresis (dgge) and gas-liquid chromatography of short chain fa ... | 2012 | 22542995 |
| in vitro inhibition of expression of virulence genes responsible for colonization and systemic spread of enteric pathogens using bifidobacterium bifidum secreted molecules. | enteric pathogens such as salmonella enterica serovar typhimurium and enterohaemorrhagic escherichia coli require an initial indispensable step of attachment or invasion of enterocytes before they can produce systemic disease and translocate to their target organs. prevention of either of these steps will result in an avirulent state and limit their pathogenicity. in vitro tests demonstrated that molecules secreted by bifidobacterium bifidum interfere with both attachment and invasion. the main ... | 2012 | 22541391 |
| effect of bifidobacterium administration on very-low-birthweight infants. | the aim of this study was to evaluate the efficacy and safety of early administration of bifidobacterium bifidum olb6378 (b. bifidum) on accelerating enteral feeding and bacterial colonization in very-low-birthweight (vlbw) infants. | 2012 | 22507386 |
| randomized controlled trial of probiotics to reduce common cold in schoolchildren. | the common cold is responsible for the largest proportion of school and work absenteeism and causes a huge economic burden. none of the current interventions is greatly effective for prevention. our aim was to assess the efficacy of a two-strain combination probiotic for prevention of common cold symptoms in healthy schoolchildren. | 2012 | 22507276 |
| breastfed infants metabolize perchlorate. | bifidobacteria are the dominant intestinal bacteria in breastfed infants. it is known that they can reduce nitrate. although no direct experiments have been conducted until now, inferred pathways for bifidobacterium bifidum include perchlorate reduction via perchlorate reductase. we show that when commercially available strains of bifidobacteria are cultured in milk, spiked with perchlorate, perchlorate is consumed. we studied 13 breastfed infant-mother pairs who provided 43 milk samples and 39 ... | 2012 | 22497505 |
| probiotic modulation of dendritic cells co-cultured with intestinal epithelial cells. | to investigate cytokine production and cell surface phenotypes of dendritic cells (dc) in the presence of epithelial cells stimulated by probiotics. | 2012 | 22493544 |
| inter-species differences in the growth of bifidobacteria cultured on human milk oligosaccharides. | human milk (hm) contains as the third most abundant component around 200 different structures of human milk oligosaccharides (hmos). hmos are the first and irreplaceable prebiotics for infants, supporting bifidobacteria as the most important bacterial group in an infant intestine. the aim of our study was to test the growth of bifidobacteria in hm and on hmos. bifidobacteria were isolated from two groups of infants. the first one (eight strains) were isolated from infants who had bifidobacteria ... | 2012 | 22491988 |
| role of extracellular transaldolase from bifidobacterium bifidum in mucin adhesion and aggregation. | the ability of bifidobacteria to establish in the intestine of mammals is among the main factors considered to be important for achieving probiotic effects. the role of surface molecules from bifidobacterium bifidum taxon in mucin adhesion capability and the aggregation phenotype of this bacterial species was analyzed. adhesion to the human intestinal cell line ht29 was determined for a collection of 12 b. bifidum strains. in four of them-b. bifidum lmg13195, dsm20456, dsm20239, and a8-the invol ... | 2012 | 22447584 |
| conjugated linoleic and linolenic acid production kinetics by bifidobacteria differ among strains. | there is great interest in conjugated linoleic acid (cla) and conjugated linolenic acid (clna) isomers because of their supposed health-promoting properties. therefore, the differences in production kinetics of cla and clna isomers from linoleic acid (la) and α-linolenic acid (α-lna), respectively, by bifidobacteria were investigated. laboratory fermentations, supplemented with la or α-lna in the fermentation medium, were performed with bifidobacterium bifidum lmg 10645, bifidobacterium breve lm ... | 2012 | 22405353 |
| reduction of patulin in aqueous solution by lactic acid bacteria. | this study aims to investigate the ability of lactic acid bacteria (lab) to remove patulin (pat) from aqueous solution with respect to the bacterial viability, initial pat concentration, incubation time, temperature, and ph. the removal of pat determined by high-performance liquid chromatography (hplc) coupled with uv detector. the maximum pat uptake was achieved by bifidobacterium bifidum 6071 and lactobacillus rhamnosus 6149 strains (52.9% and 51.1%) for viable and (54.1% and 52.0%) for nonvia ... | 2012 | 22394296 |