| comparative genome analysis of the candidate functional starter culture strains lactobacillus fermentum 222 and lactobacillus plantarum 80 for controlled cocoa bean fermentation processes. | lactobacillus fermentum 222 and lactobacillus plantarum 80, isolates from a spontaneous ghanaian cocoa bean fermentation process, proved to be interesting functional starter culture strains for cocoa bean fermentations. lactobacillus fermentum 222 is a thermotolerant strain, able to dominate the fermentation process, thereby converting citrate and producing mannitol. lactobacillus plantarum 80 is an acid-tolerant and facultative heterofermentative strain that is competitive during cocoa bean fer ... | 2015 | 26459565 |
| microbiological and physicochemical characterization of small-scale cocoa fermentations and screening of yeast and bacterial strains to develop a defined starter culture. | spontaneous cocoa bean fermentations performed under bench- and pilot-scale conditions were studied using an integrated microbiological approach with culture-dependent and culture-independent techniques, as well as analyses of target metabolites from both cocoa pulp and cotyledons. both fermentation ecosystems reached equilibrium through a two-phase process, starting with the simultaneous growth of the yeasts (with saccharomyces cerevisiae as the dominant species) and lactic acid bacteria (lab) ... | 2012 | 22636007 |
| microbial diversity and biochemical analysis of suanzhou: a traditional chinese fermented cereal gruel. | suanzhou as a traditional chinese gruel is fermented from proso millet and millet. the biochemical analysis showed suanzhou had relatively high concentrations of lactic acid, acetic acid, and free amino acids. the metagenomics of suanzhou were studied, with the analysis of the v4 region of 16s rrna gene, the genera lactobacillus and acetobacter were found dominant with the average abundance of 58.2 and 24.4%, respectively; and with the analysis of the its1 region between 18s and 5.8s rrna genes, ... | 2016 | 27610102 |
| biomedical applications of fermenticin hv6b isolated from lactobacillus fermentum hv6b mtcc10770. | fermenticin hv6b is a class iia antimicrobial peptide produced by lactobacillus fermentum hv6b mtcc 10770 isolated from human vaginal ecosystem. it shows growth inhibition of a wide range of opportunistic pathogens of humans, for example, bacteroides, gardnerella vaginalis, mobiluncus, staphylococci, and streptococci, associated with bacterial vaginosis in humans. it does possess an impressive sperm immobilization and spermicidal activity tested against human sperms which makes it an attractive ... | 2013 | 23984320 |
| lactobacillus fermentum atcc 23271 displays in vitro inhibitory activities against candida spp. | lactobacilli are involved in the microbial homeostasis in the female genital tract. due to the high prevalence of many bacterial diseases of the female genital tract and the resistance of microorganisms to various antimicrobial agents, alternative means to control these infections are necessary. thus, this study aimed to evaluate the probiotic properties of well-characterized lactobacillus species, including l. acidophilus (atcc 4356), l. brevis (atcc 367), l. delbrueckii ssp. delbrueckii (atcc ... | 2016 | 27833605 |
| the evolution and population structure of lactobacillus fermentum from different naturally fermented products as determined by multilocus sequence typing (mlst). | lactobacillus fermentum is economically important in the production and preservation of fermented foods. a repeatable and discriminative typing method was devised to characterize l. fermentum at the molecular level. the multilocus sequence typing (mlst) scheme developed was based on analysis of the internal sequence of 11 housekeeping gene fragments (clpx, dnaa, dnak, groel, murc, mure, pepx, pyrg, reca, rpob, and uvrc). | 2015 | 25990318 |
| high-throughput identification of the microbial biodiversity of cocoa bean fermentation by maldi-tof ms. | matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) is a powerful biotyping tool increasingly used for high-throughput identification of clinical microbial isolates, however, in food fermentation research this approach is still not well established. this study examines the microbial biodiversity of cocoa bean fermentation based on the isolation of micro-organisms in cocoa-producing regions, followed by maldi-tof ms in switzerland. a preceding 6-week storag ... | 2016 | 27454854 |
| hanseniaspora opuntiae, saccharomyces cerevisiae, lactobacillus fermentum, and acetobacter pasteurianus predominate during well-performed malaysian cocoa bean box fermentations, underlining the importance of these microbial species for a successful cocoa bean fermentation process. | two spontaneous malaysian cocoa bean box fermentations (one farm, two plantation plots) were investigated. physical parameters, microbial community dynamics, yeast and bacterial species diversity [mainly lactic acid bacteria (lab) and acetic acid bacteria (aab)], and metabolite kinetics were monitored, and chocolates were produced from the respective fermented dry cocoa beans. similar microbial growth and metabolite profiles were obtained for the two fermentations. low concentrations of citric a ... | 2013 | 23664257 |
| the microbiology of bandji, palm wine of borassus akeassii from burkina faso: identification and genotypic diversity of yeasts, lactic acid and acetic acid bacteria. | to investigate physicochemical characteristics and especially genotypic diversity of the main culturable micro-organisms involved in fermentation of sap from borassus akeassii, a newly identified palm tree from west africa. | 2012 | 22979949 |
| molecular identification and physiological characterization of yeasts, lactic acid bacteria and acetic acid bacteria isolated from heap and box cocoa bean fermentations in west africa. | yeast, lactic acid bacteria (lab) and acetic acid bacteria (aab) populations, isolated from cocoa bean heap and box fermentations in west africa, have been investigated. the fermentation dynamicswere determined by viable counts, and 106 yeasts, 105 lab and 82 aab isolateswere identified by means of rep-pcr grouping and sequencing of the rrna genes. during the box fermentations, the most abundant species were saccharomyces cerevisiae, candida ethanolica, lactobacillus fermentum, lactobacillus pla ... | 2016 | 26425801 |
| elucidating and regulating the acetoin production role of microbial functional groups in multispecies acetic acid fermentation. | acetoin (3-hydroxy-2-butanone) formation in vinegar microbiota is crucial for the flavor quality of zhenjiang aromatic vinegar, a traditional vinegar produced from cereals. however, the specific microorganisms responsible for acetoin formation in this centuries-long repeated batch fermentation have not yet been clearly identified. here, the microbial distribution discrepancy in the diacetyl/acetoin metabolic pathway of vinegar microbiota was revealed at the species level by a combination of meta ... | 2016 | 27451452 |
| the effect of lactic acid bacteria on cocoa bean fermentation. | cocoa beans (theobroma cacao l.) are the raw material for chocolate production. fermentation of cocoa pulp by microorganisms is crucial for developing chocolate flavor precursors. yeasts conduct an alcoholic fermentation within the bean pulp that is essential for the production of good quality beans, giving typical chocolate characters. however, the roles of bacteria such as lactic acid bacteria and acetic acid bacteria in contributing to the quality of cocoa bean and chocolate are not fully und ... | 2015 | 25889523 |
| yeasts are essential for cocoa bean fermentation. | cocoa beans (theobroma cacao) are the major raw material for chocolate production and fermentation of the beans is essential for the development of chocolate flavor precursors. in this study, a novel approach was used to determine the role of yeasts in cocoa fermentation and their contribution to chocolate quality. cocoa bean fermentations were conducted with the addition of 200ppm natamycin to inhibit the growth of yeasts, and the resultant microbial ecology and metabolism, bean chemistry and c ... | 2014 | 24462702 |
| influencing cocoa flavour using pichia kluyveri and kluyveromyces marxianus in a defined mixed starter culture for cocoa fermentation. | the potential impact of aromatic and pectinolytic yeasts on cocoa flavour was investigated using two defined mixed starter cultures encompassing strains of pichia kluyveri and kluyveromyces marxianus for inoculating cocoa beans in small scale tray fermentations. samples for microbial and metabolite analysis were collected at 12-24 hour intervals during 120 h of fermentation. yeast isolates were grouped by (gtg)5-based rep-pcr fingerprinting and identified by sequencing of the d1/d2 region of the ... | 2013 | 23866910 |
| antibiotic resistance and capacity for biofilm formation of different bacteria isolated from endodontic infections associated with root-filled teeth. | to date, a variety of microbial species have been isolated from endodontic infections. however, endodontic clinical bacterial isolates have not been sufficiently characterized with regard to their capacity for antibiotic resistance and biofilm formation. in this study, antibiotic resistance and biofilm formation of 47 different aerobic and anaerobic bacterial isolates, belonging to 32 different species previously isolated from infected filled root canals, were studied. | 2014 | 24461408 |
| irradiation effects of low temperature multi gas plasma jet on oral bacteria. | the purpose of this study is to evaluate the sterilization effects of a newly developed low temperature multi gas plasma jet on oral pathogenic microorganisms (streptococcus mutans [s. mutans], lactobacillus fermentum [l. fermentum], aggregatibacter actinomycetemcomitans [a. actinomycetemcomitans]). plasma gas which generated from o2, n2, ar and 50% (o2+n2) was irradiated to the microbes. effect of o2 plasma irradiation on s. mutans under scanning electron microscopy (sem) was also observed. o2 ... | 2016 | 27725521 |
| ethanol production by selected intestinal microorganisms and lactic acid bacteria growing under different nutritional conditions. | to gain some specific insight into the roles microorganisms might play in non-alcoholic fatty liver disease (nafld), some intestinal and lactic acid bacteria and one yeast (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, enterococcus fecalis, escherichia coli, lactobacillus acidophilus, lactobacillus fermentum, lactobacillus plantarum, weissella confusa, saccharomyces cerevisiae) were characterized by high performance liquid chromatography for production of ethanol whe ... | 2016 | 26858714 |
| antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production. | in this study, 13 lactic acid bacteria (lab) strains (including 5 lactobacillus casei, 2 lactobacillus rhamnosus, 2 lactobacillus fermentum, 1 lactobacillus acidophilus, 1 lactobacillus plantarum, 1 lactobacillus sakei, and 1 lactobacillus reuteri species) were assessed for both their antifungal activity against four food spoilage molds (colletotrichum gloeosporioides, botrytis cinerea, penicillium expansum, and aspergillus flavus) and their capability to produce the novel antimicrobial compound ... | 2014 | 24412414 |
| effect of magnesium acetate on the antimold activity of lactobacillus. | the antimold activity of lactic acid bacteria (lab) is used in food biopreservation. the aim of this study was to evaluate the effect of magnesium acetate added to de man rogosa sharpe (mrs) medium on the antimold activity of three lab strains ( lactobacillus plantarum , lactobacillus brevis , and lactobacillus fermentum ) against molds contaminating food ( aspergillus oryzae , aspergillus niger , penicillium chrysogenum , fusarium avenaceum , and rhizopus arrhizus ) and their ability to produce ... | 2017 | 28221876 |
| improvement of the antifungal activity of lactic acid bacteria by addition to the growth medium of phenylpyruvic acid, a precursor of phenyllactic acid. | the aim of the current study was to improve the antifungal activity of eight lactic acid bacterial (lab) strains by the addition of phenylpyruvic acid (ppa), a precursor of the antifungal compound phenyllactic acid (pla), to a defined growth medium (dm). the effect of ppa addition on the labs antifungal activity related to the production of organic acids (pla, d-lactic, l-lactic, acetic, citric, formic and 4-hydroxy-phenyllactic acids) and of other phenylpyruvic-derived molecules, was investigat ... | 2016 | 26827290 |
| inhibition of mycotoxin-producing aspergillus nomius vsc 23 by lactic acid bacteria and saccharomyces cerevisiae. | the effect of different fermenting microorganisms on growth of a mycotoxin- producing aspergillus nomius was assayed. two lactic acid bacteria, lactobacillus fermentum and lactobacillus rhamnosus, and saccharomyces cerevisiae, all of which are widely used in fermentation and preservation of food, were assayed on their fungus inhibitory properties. assays were carried out by simultaneous inoculation of one of the possible inhibiting microorganisms and the fungus or subsequent inoculation of one o ... | 2010 | 24031582 |
| monitoring of the microbial communities involved in the soy sauce manufacturing process by pcr-denaturing gradient gel electrophoresis. | soy sauce is a traditional seasoning produced through the fermentation of soybeans and wheat using microbes. in this study, the microbial communities involved in the soy sauce manufacturing process were analyzed by pcr-denaturing gradient gel electrophoresis (pcr-dgge). the bacterial dgge profile indicated that the bacterial microbes in the koji were weissella cibaria (weissella confusa, weissella kimchii, weissella salipiscis, lactobacillus fermentum, lactobacillus plantarum, lactobacillus iner ... | 2012 | 22475947 |
| aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity. | liamocins are structurally unique, heavier-than-water “oils” produced by certain strains of aureobasidium pullulans. the aim of the current study is to identify new sources of liamocins and evaluate their potential as anticancer agents. nine strains of a. pullulans from phylogenetic clades 8, 9, and 11 were examined for the first time for production of liamocins. strains in these clades have only been isolated from tropical environments, and all strains tested here were from various locations in ... | 2014 | 24659335 |
| effect of white mustard essential oil on the growth of foodborne pathogens and spoilage microorganisms and the effect of food components on its efficacy. | antimicrobial preservative compounds are added to foods to target specific pathogens and spoilage organisms. white mustard essential oil (wmeo) is an extract that contains 4-hydroxybenzyl isothiocyanate, a compound which has been demonstrated to have antimicrobial activity in limited studies. the objective of this research was to determine the in vitro antimicrobial activity of wmeo against gram-positive and gram-negative spoilage and pathogenic bacteria and determine the effect of food componen ... | 2014 | 25474051 |
| effect of temperature on continuous fermentative lactic acid (la) production and bacterial community, and development of la-producing uasb reactor. | a frequently used fermentation manner in lactic acid (la) production, batch fermentation by pure cultures, has a limited practicability: low volumetric productivity and high energy consumption. in this study, continuous la fermentation was performed in a completely stirred tank reactor at 12h hrt, inoculated with anaerobic digester sludge. glucose (25 g cod/l) was used as a feedstock and temperature was increased from 35 to 60°c. la production significantly increased from 50°c, which was negligi ... | 2012 | 22750503 |
| inhibitors of biofilm formation by biofuel fermentation contaminants. | biofuel fermentation contaminants such as lactobacillus sp. may persist in production facilities by forming recalcitrant biofilms. in this study, biofilm-forming strains of lactobacillus brevis, lactobacillus fermentum, and lactobacillus plantarum were isolated and characterized from a dry-grind fuel ethanol plant. a variety of potential biofilm inhibitors were tested, including microbial polysaccharides, commercial enzymes, ferric ammonium citrate, liamocins, phage endolysin, xylitol, and cultu ... | 2014 | 25022836 |
| efficient production of d-tagatose using a food-grade surface display system. | d-tagatose, a functional sweetener, is commonly transformed from d-galactose by l-arabinose isomerase (l-ai). in this study, a novel type of biocatalyst, l-ai from lactobacillus fermentum cgmcc2921 displayed on the spore surface of bacillus subtilis 168, was developed for producing d-tagatose. the anchored l-ai, exhibiting the relatively high bioactivity, suggested that the surface display system using cotx as the anchoring protein was successfully constructed. the stability of the anchored l-ai ... | 2014 | 24979201 |
| effects of replacing soybean meal with fermented rapeseed meal on performance, serum biochemical variables and intestinal morphology of broilers. | this trial was performed to study the effects of replacing soybean meal (sbm) with fermented rapeseed meal (rsm) on growth performance, serum biochemistry variable and intestinal morphology of broilers. a total of 640 d-old arbor acres broiler chicks were randomly allocated to 4 dietary treatments, 4 pens per treatment and 40 birds per pen for a 6-wk feeding trial. in the four treatment groups, fermented rsm replaced soybean meal at 0, 5, 10, and 15%, respectively. on 21 d and 42 d, two birds fr ... | 2012 | 25049539 |
| chlorine dioxide against bacteria and yeasts from the alcoholic fermentation. | the ethanol production in brazil is carried out by fed-batch or continuous process with cell recycle, in such way that bacterial contaminants are also recycled and may be troublesome due to the substrate competition. addition of sulphuric acid when inoculum cells are washed can control the bacterial growth or alternatively biocides are used. this work aimed to verify the effect of chlorine dioxide, a well-known biocide for bacterial decontamination of water and equipments, against contaminant ba ... | 2008 | 24031227 |
| characterization of cinnamoyl esterases from different lactobacilli and bifidobacteria. | a high variety of plants that are used for food production contain esterified hydroxycinnamic acids. as their free forms display several benefits, like an enhanced absorption in human intestinal tract, anti-oxidative and anti-carcinogenic effects, an improved protein solubility and reduced discoloration, the microbial ability to cleave the ester bond is highly desired. in order to examine potential fermentation strains for this purpose, six different lactic acid bacteria and one bifidobacterial ... | 2017 | 27999938 |
| human intestinal mucosa-associated lactobacillus and bifidobacterium strains with probiotic properties modulate il-10, il-6 and il-12 gene expression in thp-1 cells. | lactobacilli and bifidobacteria are considered one of the permanent genera of the physiological human intestinal microbiota and represent an enormous pool of potential probiotic candidates. approximately 450 isolates of presumptive lactobacillus or bifidobacterium strains were obtained from bioptic samples of colonic and ileal mucosa from 15 adolescents aged 12 to 18 years. on the basis of randomly amplified polymorphic dna (rapd)-pcr analysis, 20 strains were selected for further taxonomic clas ... | 2015 | 25391349 |
| synergistic antibacterial efficacies of the combination of bovine lactoferrin or its hydrolysate with probiotic secretion in curbing the growth of meticillin-resistant staphylococcus aureus. | the occurrence of multidrug-resistant or meticillin-resistant staphylococcus aureus (mrsa) has become an important issue in clinics. this study evaluated a combinatorial treatment approach by using the well-documented antibacterial protein apo-bovine lactoferrin (apo-blf) or its hydrolysate and specific probiotic supernatants for controlling mrsa infection. clinical mrsa strains were isolated from different patient specimens. apo-blf-hydrolysate possessed stronger anti-mrsa activity than complet ... | 2013 | 24072764 |
| antifungal activity and aflatoxin degradation of bifidobacterium bifidum and lactobacillus fermentum against toxigenic aspergillus parasiticus. | food and feedstuff contamination with aflatoxins (afts) is a serious health problem for humans and animals, especially in developing countries. the present study evaluated antifungal activities of two lactic acid bacteria (lab) against growth and aflatoxin production of toxigenic aspergillus parasiticus. the mycelial growth inhibition rate of a. parasiticus ptcc 5286 was investigated in the presence of bifidobacterium bifidum ptcc 1644 and lactobacillus fermentum ptcc 1744 by the pour plate meth ... | 2016 | 28077976 |
| effect of probiotic supplementation on cognitive function and metabolic status in alzheimer's disease: a randomized, double-blind and controlled trial. | alzheimer's disease (ad) is associated with severe cognitive impairments as well as some metabolic defects. scant studies in animal models indicate a link between probiotics and cognitive function. this randomized, double-blind, and controlled clinical trial was conducted among 60 ad patients to assess the effects of probiotic supplementation on cognitive function and metabolic status. the patients were randomly divided into two groups (n = 30 in each group) treating with either milk (control gr ... | 2016 | 27891089 |
| eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella. | listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ... | 2016 | 27002648 |
| polysaccharide from seeds of plantago asiatica l. affects lipid metabolism and colon microbiota of mouse. | polysaccharide from the seeds of plantago asiatica l. was given via oral administration to mice (0.4 g/kg body weight, 30 days) to observe its effects on mouse nutrient metabolism and colon microbiota. it was found the polysaccharide intake could lower the apparent absorption of lipid. total triglyceride, cholesterol, and atherogenic index in blood serum with total lipid and cholesterol levels in liver of polysaccharide group mice were all significantly lower than those of the control group (p < ... | 2014 | 24341731 |
| role of probiotics in the prevention and treatment of meticillin-resistant staphylococcus aureus infections. | meticillin-resistant staphylococcus aureus (mrsa) is a multidrug-resistant micro-organism and is the principal nosocomial pathogen worldwide. following initial in vitro experiments demonstrating that lactobacillus acidophilus cl1285(®) and lactobacillus casei lbc80r(®) commercial strains exhibit antibacterial activity against clinical mrsa isolates, we conducted a literature search to find any evidence of probiotic efficacy in decolonisation or treatment of s. aureus infection. as summarised bel ... | 2013 | 24071026 |
| antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. | previous reports have shown that several probiotic strains can resist the antibacterial activity of bovine lactoferrin (blf), but the results are inconsistent. moreover, a portion of orally administered apo-blf is digested in vivo by pepsin to yield blf hydrolysate, which produces stronger antibacterial activity than that observed with apo-blf. however, whether blf hydrolysate affects the growth of probiotic strains is unclear. therefore, various probiotic strains in taiwan were collected and ev ... | 2013 | 23332852 |
| antimicrobial activity of selected synbiotics targeted for the elderly against pathogenic escherichia coli strains. | the aim of the present study was to evaluate the antimicrobial activity of two synbiotic combinations, lactobacillus fermentum with short-chain fructooligosaccharides (fos-lf) and bifidobacterium longum with isomaltooligosaccharides (imo-bl), against enterohaemorrhagic escherichia coli o157:h7 and enteropathogenic e. coli o86. antimicrobial activity was determined (1) by co-culturing the synbiotics and pathogens in batch cultures, and (2) with the three-stage continuous culture system (gut model ... | 2016 | 26754553 |
| an in vitro study of the effect of probiotics, prebiotics and synbiotics on the elderly faecal microbiota. | the use of dietary intervention in the elderly in order to beneficially modulate their gut microbiota has not been extensively studied. the influence of two probiotics (bifidobacterium longum and lactobacillus fermentum) and two prebiotics [isomaltooligosaccharides (imo) and short-chain fructooligosaccharides (fos)], individually and in synbiotic combinations (b. longum with imo, l. fermentum with fos) on the gut microbiota of elderly individuals was investigated using faecal batch cultures and ... | 2014 | 24685554 |
| strain-dependent augmentation of tight-junction barrier function in human primary epidermal keratinocytes by lactobacillus and bifidobacterium lysates. | in this study, we investigated whether probiotic lysates can modify the tight-junction function of human primary keratinocytes. the keratinocytes were grown on cell culture inserts and treated with lysates from bifidobacterium longum, lactobacillus plantarum, lactobacillus reuteri, lactobacillus fermentum, or lactobacillus rhamnosus gg. with the exception of l. fermentum (which decreased cell viability), all strains markedly enhanced tight-junction barrier function within 24 h, as assessed by me ... | 2013 | 23770906 |
| development of antimicrobial synbiotics using potentially-probiotic faecal isolates of lactobacillus fermentum and bifidobacterium longum. | the aims of the present study were to investigate in vitro the antimicrobial activity of lactobacillus fermentum and bifidobacterium longum, isolated from faeces of healthy elderly individuals, against enterohaemorrhagic escherichia coli (e. coli o157:h7) and enteropathogenic e. coli (e. coli o86), to determine the capability of the selected strains to tolerate acid and bile in vitro, to select suitable carbohydrates in order to enhance the growth and maximise antimicrobial activity of the putat ... | 2013 | 23354005 |
| lactobacillus paracasei modulates the immune system of galleria mellonella and protects against candida albicans infection. | probiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. using the non-vertebrate model host galleria mellonella, we evaluated whether clinical isolates of lactobacillus spp. are able to provide protection against candida albicans infection. among different strains of lactobacillus paracasei, lactobacillus rhamnosus and lactobacillus fermentum, we verified that l. paracasei 28.4 strain had the greatest ability ... | 2017 | 28267809 |
| inhibitory effects of lactobacillus fermentum on microbial growth and biofilm formation. | beneficial effects of lactobacilli have been reported, and lactic bacteria are employed for conservation of foods. therefore, the effects of a lactobacillus fermentum strain were analyzed regarding inhibitory effects on staphylococci, candida albicans and enterotoxigenic enterobacteria by transmission electron microscopy (tem). tem of bacterial biofilms was performed using cocultures of bacteriocin-producing l. fermentum 97 with different enterotoxigenic strains: staphylococcus epidermidis expre ... | 2015 | 26267163 |
| detection and partial characterization of a bacteriocin-like substance produced by lactobacillus fermentum cs57 isolated from human vaginal secretions. | lactobacilli (150) from human vaginal secretions were tested for the production of antimicrobial substances which can provide a physiological defense against the pathogenic microorganisms in the vaginal area. sixteen of the isolates (10.6%) showed antibacterial activity against one or several closely related microorganisms used as indicators. lactobacillus fermentum cs57 was the best producer and secretes a bacteriocin-like substance (bls) with antagonistic activity against streptococcus agalact ... | 2014 | 24462825 |
| effectiveness of the association of 2 probiotic strains formulated in a slow release vaginal product, in women affected by vulvovaginal candidiasis: a pilot study. | vulvovaginal candidiasis (vvc) is the second most common cause of vaginitis after bacterial vaginosis, and it is diagnosed in up to 40% of women with vaginal complaints in the primary care setting. among candida spp., candida albicans is the most common infectious agent. the treatment of choice for uncomplicated vvc is achieved with single-dose or short-course therapy in over 90% of cases. several topical and oral drugs are available, without evidence for superiority of any agent or route of adm ... | 2012 | 22955364 |
| the in vitro effectiveness of lactobacillus fermentum against different candida species compared with broadly used azoles. | to investigate the possible use of lactobacillus strains in the prophylaxis and/or adjuvant therapy of acute vulvovaginal candidiasis and other vaginal infections sustained by candida yeasts. | 2016 | 27741168 |
| salivary microbiome of an urban indian cohort and patterns linked to subclinical inflammation. | to profile salivary microbiomes of an urban-living, healthy indian cohort and explore associations with proinflammatory status. | 2017 | 28383789 |
| new gene responsible for para-aminobenzoate biosynthesis. | folate is an essential cofactor in all living cells for one-carbon transfer reactions. para-aminobenzoate (paba), a building block of folate, is usually derived from chorismate in the shikimate pathway by reactions of aminodeoxychorismate synthase (paba and -b) and 4-amino-4-deoxychorismate lyase (pabc). we previously suggested that an alternative pathway for paba biosynthesis would operate in some microorganisms such as lactobacillus fermentum and nitrosomonas europaea since these bacteria show ... | 2014 | 23972426 |
| experimental application of lactobacillus fermentum ccm 7421 in combination with chlorophyllin in dogs. | chlorophyll belongs in a larger class of phytochemical plant pigments currently receiving more attention as a physiologically active dietary component. although most research has focused on its biological activities such as its antioxidant, antimutagenic, anti-inflammatory or apoptotic effects in humans or rodents, there is limited knowledge at this time about the combinative possibilities of chlorophyll with probiotic bacteria. our aim was to test the growth characteristics of canine-derived pr ... | 2015 | 26062536 |
| a canine-specific probiotic product in treating acute or intermittent diarrhea in dogs: a double-blind placebo-controlled efficacy study. | a double-blind placebo-controlled intervention study on 60 dogs recruited from a pool of canine patients visiting a veterinary practice and diagnosed with acute diarrhea was conducted. the dogs received in randomized manner either a sour-milk product containing three canine-derived lactobacillus sp. probiotics in combination of lactobacillus fermentum vet 9a, l. rhamnosus vet 16a, and l. plantarum vet 14a (2×10(9)cfu/ml), or placebo. stool consistency, general well-being, and the numbers of spec ... | 2016 | 27938673 |
| pathogen exclusion properties of canine probiotics are influenced by the growth media and physical treatments simulating industrial processes. | manufacturing process used in preparation of probiotic products may alter beneficial properties of probiotics. the effect of different growth media and inactivation methods on the protective properties of canine-originated probiotic bacteria against adhesion of canine enteropathogens was investigated. | 2014 | 24779583 |
| reduced lesions in chickens with clostridium perfringens-induced necrotic enteritis by lactobacillus fermentum 1.20291. | previous studies have demonstrated that lactobacillus has anti-inflammatory properties, but the protective functions of lactobacillus and mechanisms of inhibition of necrotic enteritis (ne) in the intestines of chickens have not been fully clarified. in the present study, we selected a probiotic strain, lactobacillus fermentum 1.2029, which has good adhesive ability and a high survival rate in low ph and bile salts. the objective of this study was to examine the anti-inflammatory properties of l ... | 2012 | 23155014 |
| bacteriocin production, antibiotic susceptibility and prevalence of haemolytic and gelatinase activity in faecal lactic acid bacteria isolated from healthy ethiopian infants. | the objective of this study was to characterise lactic acid bacteria (lab) isolated from faecal samples of healthy ethiopian infants, with emphasis on bacteriocin production and antibiotic susceptibility. one hundred fifty lab were obtained from 28 healthy ethiopian infants. the isolates belonged to lactobacillus (81/150), enterococcus (54/150) and streptococcus (15/150) genera. lactobacillus species were more abundant in the breast-fed infants while enterococcus dominated the mixed-fed populati ... | 2013 | 23184155 |
| detailed analyses of the bacterial populations in processed cocoa beans of different geographic origin, subject to varied fermentation conditions. | the quality of chocolate is influenced by several parameters, one of which is bacterial diversity during fermentation and drying; a crucial factor for the generation of the optimal cocoa flavor precursors. our understanding of the bacterial populations involved in chocolate fermentation can be improved by the use of high-throughput sequencing technologies (hts), combined with pcr amplification of the 16s rrna subunit. here, we have conducted a high-throughput assessment of bacterial diversity in ... | 2016 | 27458718 |
| presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of indian origin. | lactic acid bacteria (lab) resistant to erythromycin were isolated from different food samples on selective media. the isolates were identified as enterococcus durans, enterococcus faecium, enterococcus lactis, enterococcus casseliflavus, lactobacillus salivarius, lactobacillus reuteri, lactobacillus plantarum, lactobacillus fermentum, pediococcus pentosaceus and leuconostoc mesenteroides. of the total 60 isolates, 88 % harbored the ermb gene. the efflux gene msra was identified in e. faecium, e ... | 2012 | 22644346 |
| effect of probiotic bacteria on phagocytosis and respiratory burst activity of blood polymorphonuclear leukocytes (pmnl) in mice infected with trichinella spiralis. | this study focusses on the effect of probiotic (bacteriocinogenic) strains on parasite infection and innate immunity - phagocytosis and oxidative burst of blood monocytes and polymorphonuclear leukocytes (pmnl) in mice infected with trichinella spiralis. bacteriocinogenic and probiotic strains of different origin (enterococcus faecium al41=ccm8558, enterococcus durans ed26e/7, lactobacillus fermentum ad1=ccm7421, lactobacillus plantarum 17l/1) were administered daily in dose of 10(9)cfu/ml in 10 ... | 2016 | 27425573 |
| microbiological characterization using combined culture dependent and independent approaches of casizolu pasta filata cheese. | casizolu is a traditional sardinian (italy) pasta filata cheese made with cow raw milk belonging to sardo-modicana and/or bruno-sarda breeds added with natural whey starter. this work aims to describe the traditional technology of this product and to evaluate the microbial groups/species involved in the first month of ripening. | 2016 | 26551888 |
| diversity of lactic acid bacteria isolated from brazilian water buffalo mozzarella cheese. | the water buffalo mozzarella cheese is a typical italian cheese which has been introduced in the thriving brazilian market in the last 10 y, with good acceptance by its consumers. lactic acid bacteria (lab) play an important role in the technological and sensory quality of mozzarella cheese. in this study, the aim was to evaluate the diversity of the autochthones viable lab isolated from water buffalo mozzarella cheese under storage. samples were collected in 3 independent trials in a dairy indu ... | 2015 | 25597646 |
| identification of lactic acid bacteria in traditional fermented yak milk and evaluation of their application in fermented milk products. | in this study, 53 strains of lactic acid bacteria (lab) isolated from xueo, a traditional fermented yak milk in the western sichuan plateau of china, were identified and their use in fermented milk was evaluated. all gram-positive and catalase-negative strains were divided into 6 groups at the level of 87% similarity using amplified ribosomal dna restriction analysis. these groups were identified as 6 species using 16s rdna sequence analysis and atpa gene analysis. the dominant lab strains in xu ... | 2012 | 22365191 |
| hydrothermally treated chitosan hydrogel loaded with copper and zinc particles as a potential micronutrient-based antimicrobial feed additive. | large-scale use of antibiotics in food animal farms as growth promoters is considered as one of the driving factors behind increasing incidence of microbial resistance. several alternatives are under investigation to reduce the amount of total antibiotics used in order to avoid any potential transmission of drug resistant microbes to humans through food chain. copper sulfate and zinc oxide salts are used as feed supplement as they exhibit antimicrobial properties in addition to being micronutrie ... | 2015 | 26664989 |
| characterization of lactobacillus fermentum pl9988 isolated from healthy elderly korean in a longevity village. | in this work, we wanted to develop a probiotic from famous longevity villages in korea. we visited eight longevity villages in korea to collect fecal samples from healthy adults who were aged above 80 years and had regular bowel movements, and isolated lactic-acid-producing bacteria from the samples. isolated colonies that appeared on mrs agar containing bromophenol blue were identified by means of 16s rrna sequencing, and 102 of the isolates were identified as lactic-acid-producing bacteria (18 ... | 2015 | 26095384 |
| the influence of the wooden equipment employed for cheese manufacture on the characteristics of a traditional stretched cheese during ripening. | the influence of the wooden equipment used for the traditional cheese manufacturing from raw milk was evaluated on the variations of chemico-physical characteristics and microbial populations during the ripening of caciocavallo palermitano cheese. milk from two farms (a, extensive; b, intensive) was processed in traditional and standard conditions. chemical and physical traits of cheeses were affected by the farming system and the cheese making technology, and changed during ripening. content in ... | 2015 | 25475270 |
| characterization of lactic acid bacteria isolated from infant faeces as potential probiotic starter cultures for fermented sausages. | a total of 109 lactic acid bacteria isolated from infant faeces were identified by partial 16s rrna, cpn60 and/or phes sequencing. lactobacillus was the most prevalent genus, representing 48% of the isolates followed by enterococcus (38%). lactobacillus gasseri (21%) and enterococcus faecalis (38%) were the main species detected. a further selection of potential probiotic starter cultures for fermented sausages focused on lactobacillus as the most technologically relevant genus in this type of p ... | 2014 | 24290655 |
| influence of different probiotic lactic acid bacteria on microbiota and metabolism of rats with dysbiosis. | lactic acid bacteria (lab) are often used for prevention and treatment of dysbiosis. however, the action of various strains of lab on metabolism and digestion under these conditions are poorly understood. the purpose of this study was to investigate the influence of probiotic lab on metabolism, digestion and microbiota in animals with dysbiosis. after administration of ampicillin and metronidazole male wistar rats, were fed products containing enterococcus faecium l3 (e.f.), lactobacillus fermen ... | 2013 | 24936361 |
| characteristics of lactic acid bacteria isolates and their effect on silage fermentation of fruit residues. | the natural lactic acid bacteria (lab) population, chemical composition, and silage fermentation of fruit residues were studied. eighty-two strains of lab were isolated from fruit residues such as banana leaf and stem, pineapple peel, and papaya peel. all strains were gram-positive and catalase-negative bacteria, and they were divided into 7 groups (a-g) according to morphological and biochemical characters. strains in groups a to f were rods, and group g was cocci. group f produced gas from glu ... | 2016 | 27108171 |
| influence of pdo ragusano cheese biofilm microbiota on flavour compounds formation. | the objectives of the present study were to characterize the biofilm microbiota of 11 different farms (from a to k), producing pdo ragusano cheese, and to investigate on its ability to generate volatile organic compounds (vocs) in milk samples inoculated with biofilm and incubated under ragusano cheese making conditions. the biofilms were subjected to plate counting and pcr/t/dgge analysis and the vocs generated in incubated milk samples were evaluated through smartnose, gc/o, and gc/ms. strepto ... | 2017 | 27697162 |
| postnatal regulation of mamdc4 in the porcine intestinal epithelium is influenced by bacterial colonization. | the mam domain-containing 4 (mamdc4) protein is associated with the unique endocytotic mechanism observed in the intestine of mammals during the immediate postnatal period. transcriptional expression of mamdc4 was substantially upregulated at birth in both the piglet jejunum and ileum and its expression decreases after birth. the protein was found localized specifically to the apical region of the luminal epithelium, however, mamdc4 protein expression was lost at day 10 and 15 in the jejunum and ... | 2016 | 27821716 |
| synbiotics suppress the release of lactate dehydrogenase, promote non-specific immunity and integrity of jejunum mucosa in piglets. | the aim of our experiment was to study how synbiotics are able to deal with the problems of post-weaning piglets. lactobacillus plantarum - biocenol(tm) lp96 (ccm 7512), lactobacillus fermentum - biocenol(tm) lf99 (ccm 7514) and flaxseed (rich in n-3 polyunsaturated fatty acids) were administered to 36 conventional piglets from a problematic breed with confirmed presence of enterotoxigenic escherichia coli and coronavirus. the experimental piglets were supplied with probiotic cheeses and crushed ... | 2016 | 27581561 |
| novel feruloyl esterase from lactobacillus fermentum nrrl b-1932 and analysis of the recombinant enzyme produced in escherichia coli. | a total of 33 lactobacillus strains were screened for feruloyl esterase (fe) activity using agar plates containing ethyl ferulate as the sole carbon source, and lactobacillus fermentum nrrl b-1932 demonstrated the strongest fe activity among a dozen species showing a clearing zone on the opaque plate containing ethyl ferulate. fe activities were monitored using high-performance liquid chromatography with an acetonitrile-trifluoroacetic acid gradient. to produce sufficient purified fe from l. fer ... | 2016 | 27260363 |
| isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics. | this study was conducted to select lactobacillus strains from various sources on the basis of their probiotic attributes, such as acid and bile tolerance, binding to intestinal cells, and antimicrobial activity. twelve isolates were obtained from human and food sources and were evaluated against standard probiotic lactobacillus rhamnosus gg (lgg). isolates were also studied for their antibiotic susceptibility. isolate lactobacillus fermentum gpi-6 showed the best survival profile at 0.3% and 1% ... | 2016 | 26928011 |
| antimicrobial activity of lactobacillus spp. isolated from fecal flora of healthy breast-fed infants against diarrheagenic escherichia coli. | among the enteric pathogens, diarrheagenic escherichia coli are important causes of diarrhea in children in both developing and industrialized countries. some lactobacillus species are commonly used as probiotics, with effects especially against acute diarrhea in childhood. | 2015 | 26865944 |
| technological properties and probiotic potential of lactobacillus fermentum strains isolated from west african fermented millet dough. | throughout africa, food fermentations are still driven by indigenous microorganisms which influence the nutritional, organoleptic and safety of the final products. however, for improved safety, consistent quality and beneficial health effects, a trend has emerged which involves the isolation of indigenous strains from traditional fermented products to be used as functional starter cultures. these functional starter cultures possess inherent functional characteristics and can contribute to food q ... | 2015 | 26560346 |
| antimicrobial activity and partial characterization of bacteriocin-like inhibitory substances produced by lactobacillus spp. isolated from artisanal mexican cheese. | lactobacillus spp. from mexican cocido cheese were shown to produce bacteriocin-like substances (bls) active against staphylococcus aureus,listeria innocua,escherichia coli, andsalmonella typhimurium by using the disk diffusion method. crude extracts of lactobacillus fermentum showed strong inhibitory activity against staph. aureus, l. innocua, e. coli, and salmonella cholerae. complete inactivation of antimicrobial activity was observed after treatment of crude extracts with proteinase k, prona ... | 2015 | 26476937 |
| heat-killed lactobacillus spp. cells enhance survivals of caenorhabditis elegans against salmonella and yersinia infections. | this study examined the effect of feeding heat-killed lactobacillus cells on the survival of caenorhabditis elegans nematodes after salmonella typhimurium and yersinia enterocolitica infection. the feeding of heat-killed lactobacillus plantarum 133 (lp133) and lactobacillus fermentum 21 (lp21) cells to nematodes was shown to significantly increase the survival rate as well as stimulate the expression of pmk-1 gene that key factor for c. elegans immunity upon infection compared with control nemat ... | 2015 | 26250615 |
| phage-mediated transfer of a dextranase gene in lactobacillus sanfranciscensis and characterization of the enzyme. | while phages of lactobacilli are extensively studied with respect to their structure and role in the dairy environment, knowledge about phages in bacteria residing in sourdough fermentation is limited. based on the previous finding that the lactobacillus sanfranciscensis phage ev3 carries a putative dextranase gene (dex), we have investigated the distribution of similar dex(+) phages in l. sanfranciscensis, the chance of gene transfer and the properties of the dextranase encoded by phage ev3. l. ... | 2015 | 25771219 |
| functional analysis of the n-terminal region of endolysin lyb5 encoded by lactobacillus fermentum bacteriophage φpyb5. | lactobacillus fermentum temperate bacteriophage φpyb5 uses endolysin lyb5 and holin hyb5 to burst the host cell. previous results showed that expression of lyb5 in escherichia coli caused host cell lysis slowly, leading us to suppose that lyb5 could pass the cytoplasmic membrane partly. in this work, the function of a putative signal peptide (splyb5) at the n-terminal of lyb5 was investigated. in e. coli, the cell adopted a spherical shape during induction of lyb5 protein, while morphological ch ... | 2015 | 25770427 |
| probiotic properties of lactobacillus strains isolated from gizzard of local poultry. | the aim of our study was to characterize gizzard lactobacillus fermentum-group strains on the basis of their phenotypic profiles regarding characteristics of lactobacilli. in addition, their in vitro potential probiotic properties were evaluated. | 2014 | 25705363 |
| protein purification, crystallization and preliminary x-ray diffraction analysis of l-arabinose isomerase from lactobacillus fermentum cgmcc2921. | l-arabinose isomerase (ai) catalyzes the isomerization of l-arabinose to l-ribulose, as well as that of d-galactose to d-tagatose. a thermophilic ai derived from lactobacillus fermentum cgmcc2921 (lfai) was overexpressed in escherichia coli bl21 (de3). this enzyme was purified to over 95% purity by nickel affinity, mono-q ion-exchange and size-exclusion chromatography. the lfai protein was crystallized from either 0.1 m bis-tris ph 6.5, 23% peg 3350, 0.3 m nacl (form 1 crystals) or 0.1 m bis-tri ... | 2015 | 25615964 |
| ethenoguanines undergo glycosylation by nucleoside 2'-deoxyribosyltransferases at non-natural sites. | deoxyribosyl transferases and functionally related purine nucleoside phosphorylases are used extensively for synthesis of non-natural deoxynucleosides as pharmaceuticals or standards for characterizing and quantitating dna adducts. hence exploring the conformational tolerance of the active sites of these enzymes is of considerable practical interest. we have determined the crystal structure at 2.1 å resolution of lactobacillus helveticus purine deoxyribosyl transferase (pdt) with the tricyclic p ... | 2014 | 25521390 |
| dietary supplementation of milk fermented with probiotic lactobacillus fermentum enhances systemic immune response and antioxidant capacity in aging mice. | although probiotics are known to enhance the host immune response, their roles in modulating immunosenescence, resisting infection, and improving redox homeostasis during aging remain unclear. therefore, the present study was devised in aging mice to assess the antiimmunosenescence potential from the consumption of milk that is fermented with probiotic lactobacillus fermentum mtcc 5898 (lf). we hypothesized that probiotic supplementation would boost immunity, improve antioxidant capacity, and re ... | 2014 | 25311611 |
| effectiveness of the two microorganisms lactobacillus fermentum lf15 and lactobacillus plantarum lp01, formulated in slow-release vaginal tablets, in women affected by bacterial vaginosis: a pilot study. | bacterial vaginosis (bv) is the most common reason for abnormal vaginal discharge in reproductive-age women and one of its most important causative agents is the gram-variable bacterium gardnerella vaginalis. bv is not accompanied by significant local inflammation, whereas the "fishy odor" test is always positive. in contrast, aerobic vaginitis (av) is predominantly associated with escherichia coli, but streptococcus agalactiae and staphylococcus aureus are also involved. standard treatment of b ... | 2014 | 25291116 |
| assessment of probiotic properties in lactic acid bacteria isolated from wine. | probiotic properties are highly strain-dependent but rarely studied in enological lactic acid bacteria (lab). in this study, the probiotic features of 11 strains of lactobacillus spp., pediococcus spp., and oenococcus oeni, including saliva and acid resistance, bile tolerance and exopolysaccharides' production, were investigated. the assays included two probiotic reference strains (lactobacillus plantarum clc 17 and lactobacillus fermentum cect5716). the lactobacillus and pediococcus strains sho ... | 2014 | 25084666 |
| tailoring the probiotic potential of non-starter lactobacillus strains from ripened parmigiano reggiano cheese by in vitro screening and principal component analysis. | non-starter lactic acid bacteria (nslab) inhabiting fermented food have been recently revised as source of probiotic strains. here, we in vitro assessed the potential probiotic aptitude of a de-replicated set of nslab previously isolated from long ripened parmigiano reggiano cheeses (22 lactobacillus rhamnosus, 18 lactobacillus paracasei, 3 lactobacillus casei, 2 lactobacillus harbinensis, and 2 lactobacillus fermentum). most strains showed moderate to good resistance to biological barriers, inc ... | 2014 | 24290648 |
| function of aspartic acid residues in optimum ph control of l-arabinose isomerase from lactobacillus fermentum. | l-arabinose isomerase (l-ai) catalyzes the isomerization of l-arabinose to l-ribulose and d-galactose to d-tagatose. most reported l-ais exhibit neutral or alkaline optimum ph, which is less beneficial than acidophilic ones in industrial d-tagatose production. lactobacillus fermentum l-ai (lfai) is a thermostable enzyme that can achieve a high conversion rate for d-galactose isomerization. however, its biocatalytic activity at acidic conditions can still be further improved. in this study, we re ... | 2014 | 24220791 |
| strain-specific probiotics properties of lactobacillus fermentum, lactobacillus plantarum and lactobacillus brevis isolates from brazilian food products. | a total of 234 lab isolates from brazilian food products were initially screened for their ability to survive at ph 2.0. fifty one of the isolates survived and were selected. they were characterized by phenotypic methods, rep-pcr and identified using 16s rrna gene sequencing as lactobacillus fermentum (34 isolates), lactobacillus plantarum (10) and lactobacillus brevis (7). based on being either highly tolerant to bile, showing an ability for auto-aggregation and/or hydrophobic properties, one l ... | 2013 | 23764216 |
| molecular cloning, characterization and heterologous expression of bile salt hydrolase (bsh) from lactobacillus fermentum ncdo394. | bile salt hydrolase (bsh) active probiotic strains hydrolyze bile acid amino conjugates in vivo, which triggers cholesterol consumption in liver to synthesize new bile leading to consequential cholesterol lowering. hence, bile salt hydrolyzing potential was the criterion to select l. fermentum ncdo394 for this study and its gene encoding bsh was identified and cloned. the resulting nucleotide sequence of bsh gene contained an open reading frame (orf) of 978 nucleotides encoding a predicted prote ... | 2013 | 23673477 |
| in vitro probiotic properties of lactobacillus fermentum sk5 isolated from vagina of a healthy woman. | a lactobacillus strain isolated from a vaginal tract of a healthy woman was examined in vitro for its probiotic potential. this strain, identified as lactobacillus fermentum sk5, was able to survive at ph 3-4 and 0.1-0.2% bile, and unaffected by pepsin (3 g l(-1)) and pancreatin (1 g l(-1)), but was susceptible to all tested antibiotics except metronidazole. l. fermentum sk5 had an antimicrobial potential against gastrointestinal pathogenic escherichia coli and vaginal pathogenic gardnerella vag ... | 2013 | 23624069 |
| investigation of vaginal microbiota in sexually active women using hormonal contraceptives in pakistan. | previous studies report association of contraceptives with moderate increase in urinary tract infection among sexually active premenopausal women. the aim of our study was to find out whether the use of hormonal contraceptives has any effect on microbiota of the vagina in the contraceptives users in khairpur sindh pakistan. | 2012 | 22901000 |
| antimicrobial susceptibility of microflora from ovine cheese. | strains identified in ovine cheese and bryndza by matrix-assisted laser desorption/ionization time-of-flight analysis belonged to ten species of non-enterococcal lactic acid bacteria and included lactobacillus casei/lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus helveticus, lactobacillus delbrueckii, lactobacillus fermentum, lactobacillus brevis, lactococcus lactis, pediococcus pentosaceus and pediococcus acidilactici. the susceptibility toward antibioti ... | 2012 | 22491987 |
| functional properties of lactobacillus strains isolated from dairy products. | twenty-four acid- and bile-tolerant lactobacilli isolates from dairy products were identified and further in vitro characterized for the presence of functional traits potentially useful for probiotic applications, which included desirable and undesirable traits, such as biofilm formation, ability to inhibit intestinal pathogens, antibiotic susceptibility, and enzyme activity. the majority of examined strains were susceptible to certain antimicrobial agents (streptomycin, gentamicin, clindamycin, ... | 2012 | 22488103 |
| a selected probiotic strain of lactobacillus fermentum cm33 isolated from breast-fed infants as a potential source of β-galactosidase for prebiotic oligosaccharide synthesis. | lactic acid bacteria from healthy breast-fed infants were isolated and screened for β-galactosidase production in mrs broth. among 49 isolates that exhibited the yellow clear zone on mrs agar supplemented with bromocresol blue, the isolate cm33 was selected as being the highest β-galactosidase producer and was identified as lactobacillus fermentum based on its morphological characteristics and 16s rdna nucleotide sequence. l. fermentum cm33 exhibited a good survival rate under the simulated stom ... | 2012 | 22367946 |
| bacteriocins produced by l. fermentum and l. acidophilus can inhibit cephalosporin resistant e. coli. | reemerging infections occur due to resistant bacteria. such infections create restrictions for clinicians and microbiologists in drug selection. such problems demand new strategies for solution. use of bacteriocins for this purpose may be fruitful. in the present research work, the inhibitory effects of bactericins on cephalosporin resistant escherichia coli are used as model system for the control of antibiotic resistant pathogenic bacteria. cephalosporin resistant escherichia coli strain was i ... | 2010 | 24031540 |
| in vitro activity of lactobacillus fermentum lf5 against different candida species and gardnerella vaginalis: a new perspective to approach mixed vaginal infections? | this study was undertaken to demonstrate the ability of lactobacillus fermentum lf5 (dsm 32277) to inhibit in vitro different candida species and gardnerella vaginalis to weigh its potential effectiveness even in mixed vaginal infections. | 2016 | 27741167 |
| switches in genomic gc content drive shifts of optimal codons under sustained selection on synonymous sites. | the major codon preference model suggests that codons read by trnas in high concentrations are preferentially utilized in highly expressed genes. however, the identity of the optimal codons differs between species although the forces driving such changes are poorly understood. we suggest that these questions can be tackled by placing codon usage studies in a phylogenetic framework and that bacterial genomes with extreme nucleotide composition biases provide informative model systems. switches in ... | 2016 | 27540085 |
| curative effect of the probiotic strain lactobacillus fermentum l23 in a murine model of vaginal infection by gardnerella vaginalis. | bacterial vaginosis is a common vaginal infection characterized by changes in the vaginal microbiota. the objective of this work was to evaluate the colonization ability and curative effect of lactobacillus fermentum l23 after vaginal administration in female balb/c mice infected with gardnerella vaginalis. one dose of lact. fermentum l23 containing 10(9 ) cfu ml(-1) was administered locally in a murine vaginal model. l23 colonized the vaginal tract of balb-c mice after one inoculation. the infe ... | 2014 | 24641524 |
| lactobacillus fermentum cjl-112 protects mice against influenza virus infection by activating t-helper 1 and eliciting a protective immune response. | we have previously reported that nasally administered lactobacillus fermentum cjl-112 (cjl-112) efficiently improves resistance against lethal influenza infection in both mice and chicken. the aim of the present study was to understand the underlying mechanisms of the significant anti-influenza activity of this lactobacilli strain. in vitro, co-culturing of the chicken macrophage cell line hd-11 with cjl-112 significantly increased nitric oxide (no) production. in vivo, cjl-112 was nasally admin ... | 2014 | 24201084 |
| effect of intranasal administration of lactobacillus fermentum cjl-112 on horizontal transmission of influenza virus in chickens. | the aim of this study was to determine whether intranasal administration of lactobacillus sp. could prevent horizontal transmission of h9n2 avian influenza virus (aiv) in specific-pathogen-free chickens. three-week-old chickens received 500 μl of 1.5 × 10(9) cfu of lactobacillus fermentum cjl-112 strain (cjl) intranasally for 7 d before and 14 d after a challenge. challenged chickens, each inoculated with h9n2 aiv, were kept in either direct or indirect contact with naive chickens, and morbidity ... | 2012 | 22991537 |
| a methodological approach to screen diverse cheese-related bacteria for their ability to produce aroma compounds. | microorganisms play an important role in the development of cheese flavor. the aim of this study was to develop an approach to facilitate screening of various cheese-related bacteria for their ability to produce aroma compounds. we combined i) curd-based slurry medium incubated under conditions mimicking cheese manufacturing and ripening, ii) powerful method of extraction of volatiles, headspace trap, coupled to gas chromatography-mass spectrometry (hs-trap-gc-ms), and iii) metabolomics-based me ... | 2015 | 25475278 |
| probiotic lactobacillus fermentum uco-979c biofilm formation on ags and caco-2 cells and helicobacter pylori inhibition. | the ability of the human isolate lactobacillus fermentum uco-979c to form biofilm and synthesize exopolysaccharide on abiotic and biotic models is described. these properties were compared with the well-known lactobacillus casei shirota to better understand their anti-helicobacter pylori probiotic activities. the two strains of lactobacilli synthesized exopolysaccharide as detected by the dubois method and formed biofilm on abiotic and biotic surfaces visualized by crystal violet staining and sc ... | 2016 | 27834106 |