| empyema of the gall-bladder due to lactobacillus casei. | | 1988 | 3141519 |
| direct 19f nmr observation of the conformational selection of optically active rotamers of the antifolate compound fluoronitropyrimethamine bound to the enzyme dihydrofolate reductase. | the molecular basis of the binding of the lipophilic antifolate compound fluoronitropyrimethamine [2,4-diamino-5-(4-fluoro-3-nitrophenyl)-6-ethylpyrimidine] to its target enzyme dihydrofolate reductase has been investigated using a combination of 19f nmr spectroscopy and molecular mechanical calculations. 19f nmr reveals the presence of two different conformational states for the fluoronitropyrimethamine-lactobacillus casei enzyme complex. mm2 molecular mechanical calculations predict restricted ... | 1988 | 3142793 |
| [relationship between ph changes of mixed cariogenic bacteria in culture and incubation time]. | | 1988 | 3143659 |
| affinity labeling the dna polymerase alpha complex. identification of subunits containing the dna polymerase active site and an important regulatory nucleotide-binding site. | pyridoxal 5'-phosphate (plp) inhibits dna polymerase activity of the intact multifunctional dna polymerase alpha complex by binding at either of two sites which can be distinguished on the basis of differential substrate protection. one site (plp site 1) corresponds to an important nucleotide-binding site which is distinct from the dna polymerase active site and which appears to correspond to the dna primase active site while the second site (plp site 2) corresponds to the dntp binding domain of ... | 1988 | 3143725 |
| adherence of specific yogurt micro-organisms to human peripheral blood lymphocytes. | yogurt lactic-acid-bacteria (lab) consisting of lactobacillus bulgaricus and streptococcus thermophilus were evaluated for their capacity to bind to human peripheral blood lymphocytes (pbl). these micro-organisms adhere to human t lymphocytes, and bind to b lymphocytes, in high and low frequencies, respectively. in addition, a quantitative analysis of lab binding to pbl was carried out using the same parameters previously applied to a salmonella model. the effect of yogurt lab on the natural ant ... | 1988 | 3144641 |
| detection of a lactobacillus substance that inhibits escherichia coli. | recent studies have shown that certain lactobacilli strains have the ability to interfere with the adherence and growth of uropathogenic bacteria. this interaction is believed to be important in the maintenance of a normal urogenital flora and in the prevention of infection in females. in the present study, lactobacillus casei ssp. rhamnosus gr-1 and lactobacillus acidophilus 76 were found to exert an inhibitory effect on pyelonephritogenic mutant escherichia coli hu 734 and e. coli atcc 25922. ... | 1988 | 3145135 |
| [studies of artificial plaque: comparison of the adherence ability of 5 cariogenic bacteria in vitro]. | | 1988 | 3148416 |
| [analysis of extracellular polysaccharides synthesized by cariogenic bacteria in mixed cultures]. | | 1988 | 3149621 |
| microbial caries induction in the roots of human teeth in vitro. | streptococcus mutans gs5, lactobacillus casei dsm20011 and actinomyces viscosus t14 produce artificial caries in the roots of extracted teeth. roots were coated with wax leaving an 8 mm2 window exposed on the buccal surfaces, and then incubated for 8 days in the presence of the test organism, the synthetic medium being changed each day. samples were then examined by sem, or microradiographs were obtained from 120 microns sections. the ph at the root surface at the end of the induction averaged 4 ... | 1988 | 3149890 |
| thymidylate synthase and fluorouracil. | | 1988 | 3150251 |
| methods for thymidylate synthase pharmacodynamics: serial biopsy, free and total ts, fdump and dump, and h4pteglu and ch2-h4pteglu assays. | this report details our methods for performance of the major parameters related to quantitation of ts inhibition resulting from fluoropyrimidine administration to patients, methods equally applicable to preclinical studies. sampling of tumors before and after drug treatment is done by 4 mm disposable punch biopsy or forceps biopsy via subcutaneous tunneling. homogenates are prepared using n2 or polytron-mincing. cytosolic free ts is measured by either the tritium-release method for small biopsie ... | 1988 | 3150252 |
| the cell-bound proteinase system of lactobacillus casei--purification and characterization. | the cell-wall crude extract from lactobacillus casei ncdo 151 was partially purified by deae-sephacel chromatography. three active fractions were eluted. two major peaks (eluted with 0.05 m and 0.27 m phosphate buffer) were further investigated. peak i represented enzymatic activity with an optimum temperature of 40 degrees c, an optimum ph of 7.0 and was strongly inhibited by the serine proteinase inhibitor phenylmethylsulfonylfluoride. peak ii represented an enzymatic activity with an optimum ... | 1988 | 3152801 |
| ph change in artificial dental plaque formed by glucosyltransferase and some oral bacteria during batch and continuous culture. | streptococcus mutans alone or cell-free glucosyltransferase (g-tase)-together with either streptococcus sanguis, streptococcus salivarius, actinomyces viscosus, or lactobacillus casei cells-formed artificial dental plaque that firmly adhered to glass electrodes in a continuous culture system containing sucrose. the ph in these artificial plaque samples decreased more than did that of the surrounding medium. in the absence of gtase, the bacteria other than s. mutans did not form firmly-adhering p ... | 1985 | 3156164 |
| induction of tumoricidal peritoneal exudate cells by administration of lactobacillus casei. | the lifespan of mice into which meth a fibrosarcoma was transplanted intraperitoneally (i.p.) was prolonged by i.p. treatment with lactobacillus casei yit 9018 (lc 9018). treatment with lc9018 before the tumor inoculation (pre-treatment), was more effective than after the tumor inoculation (post-treatment). when tumor cells were inoculated subcutaneously along with peritoneal exudate cells (pec) harvested from lc 9018-treated mice in the winn type test, the tumor growth was significantly inhibit ... | 1985 | 3158616 |
| synthesis and antifolate activity of 5-methyl-5,10-dideaza analogues of aminopterin and folic acid and an alternative synthesis of 5,10-dideazatetrahydrofolic acid, a potent inhibitor of glycinamide ribonucleotide formyltransferase. | the title compounds were prepared in extensions of a general synthetic approach used earlier to prepare 5-alkyl-5-deaza analogues of classical antifolates. wittig condensation of 2,4-diaminopyrido[2,3-d]pyrimidine-6-carboxaldehyde (2a) and its 5-methyl analogue 2b with [4-(methoxycarbonyl)benzylidene] triphenylphosphorane gave 9,10-ethenyl precursors 3a and 3b. hydrogenation (dmf, ambient, 5% pd/c) of the 9,10-ethenyl group of 3b followed by ester hydrolysis led to 4-[2-(2,4-diamino-5-methylpyri ... | 1988 | 3184124 |
| cell surface charge of lactobacilli and enterococci isolated from pig small intestine as studied by free zone electrophoresis: a methodological study. | the net surface charge density, as revealed by electrophoretic mobility values, of fourteen strains of lactobacilli and two strains of enterococci isolated from the small intestine of pigs, was studied by a free zone electrophoretic technique in a rotating quartz electrophoresis tube. most lactobacilli strains of low surface hydrophobicity (i.e. hydrophilic strains) showed high electrophoretic mobility values but no visible capsule, while most strains of intermediate and high surface hydrophobic ... | 1988 | 3216801 |
| [adjuvant activity of lactic bacteria: perspectives for its use in oral vaccines]. | the effect of the oral and subcutaneous administration of lactobacillus casei, lactobacillus acidophilus, lactobacillus bulgaricus and streptococcus thermophilus on humoral antibody production and delayed type hypersensitivity response against sheep red blood cells (srbc) was studied. the species of the genus lactobacillus proved to be more effective in both cases, effects being stronger when working with viable bacteria than with non-viable cells. the stimulation of primary cellular and humoral ... | 1988 | 3231712 |
| pharmacokinetics of leucovorin calcium after intravenous, intramuscular, and oral administration. | the pharmacokinetics of leucovorin was evaluated after intravenous, intramuscular, and oral administration in a randomized crossover study of 37 healthy men. a single 25-mg dose of leucovorin calcium was administered intravenously, intramuscularly, or orally to the subjects. blood samples were obtained immediately before and at 13 time points up to 24 hours after the leucovorin dose. the three treatment phases were separated by one-week intervals. bioavailability was assessed by measuring over 2 ... | 1988 | 3257913 |
| [heat-killed lactobacillus casei, lc-9018, as an interferon inducer]. | | 1988 | 3266256 |
| susceptibility of fibronectin to degradation by various gram-negative and gram-positive oral micro-organisms. | the degradative effects on tritiated human plasma fibronectin (fn) of proteases associated with twenty five gram-negative (gn) and fifteen gram-positive (gp) oral microbial isolates were examined. ninety-two per cent of the gn and 20% of the gp isolates degraded the fn. in the gn group, the degradative effects were greatest for bacteroides gingivalis and treponema denticola, while in the gp group streptococcus faecalis produced appreciable, and the two lactobacillus casei isolates moderate and l ... | 1988 | 3287100 |
| effect of the novel ionophore tetronasin (ici 139603) on ruminal microorganisms. | the antimicrobial activity of the novel ionophore tetronasin (formerly ici 139603) was compared with that of monensin for the growth of ruminal bacteria, protozoa, and an anaerobic fungus. the potency of tetronasin toward most bacteria and the fungus was an order of magnitude or more greater than that of monensin. lactobacillus casei was 55 times more sensitive to tetronasin than to monensin, indicating a potential role for tetronasin in reversing lactic acidosis. bacteria with a gram-positive u ... | 1988 | 3355139 |
| affinity chromatography of naturally occurring folate derivatives. | in a previous report (1980, in methods in enzymology, vol. 66, p. 686, academic press, new york) we described the preparation and use of purified milk folate-binding protein covalently linked to a sepharose matrix. the present study was undertaken to test the capacity of this preparation to purify quantitatively folates from tissue extracts. in experiments that employed tissue extracts containing biologically synthesized [3h]folates, recovery of radioactivity from columns of the immobilized fola ... | 1988 | 3364725 |
| characterization of the pools of 5,10-methylenetetrahydrofolates and tetrahydrofolates in xenografts of human colon adenocarcinoma. | the method for measuring polyglutamate forms of ch2-h4pteglu and h4pteglu, by entrapment in ternary complexes with [6-3h]5-fluoro-2'-deoxyuridylate and lactobacillus casei thymidylate synthase has been characterized. results demonstrated that (a) the relationship between concentration of ch2-h4pteglu and complex isolated on nondenaturing gels was dependent upon the number of glutamyl residues, and an alternative method for data analysis has been presented, (b) the relationship was linear over a ... | 1988 | 3365696 |
| linear free energy relationship studies of enzyme active site binding: thymidylate synthase. | the requirements for active-site binding of thymidylate synthase from three sources, lactobacillus casei, murine leukemia l1210, and human lymphoblast (molt/4f), were investigated by analyzing the binding of a series of 5-(p-substituted phenyl)-2'-deoxyuridylates (n1-substituted 5-aryl-2, 4-dioxopyrimidines) to the enzyme. multiple regression analysis revealed that an increase in electron density of the heterocyclic ring and hydrophobic substituents enhance affinity. correlations of biological r ... | 1988 | 3373483 |
| effect of monoclonal antibodies against lipoteichoic acid from the oral bacterium streptococcus mutans on its adhesion and plaque-accumulation in vitro. | five monoclonal antibodies directed against streptococcus mutans strain jbp lipoteichoic acid (lta) were characterized. they were all similarly reactive with the immunizing lta-containing extract, with intact strep. mutans jbp cells and with lta purified from lactobacillus casei. immobilized anti-lta antibodies removes lta from lta-containing extracts. the binding of antibodies to lta was inhibited by the aqueous extract but not by the organic extract of de-acylated lta, indicating reactivity wi ... | 1986 | 3467668 |
| pulp response to and cariogenicity of lactobacillus casei in monoinfected gnotobiotic rats. | the maxillary molar pulps of germ-free rats were mechanically exposed and suspensions of a strain of freshly grown lactobacillus casei were applied to the pulp wounds. the pulps were left open to the oral environment, and the animals were maintained within the isolator until death. in the majority of teeth, pulp necrosis was evident and apical inflammation was present in 40% of the specimens. the remaining monoinfected animals were bred and the litters were placed on a 46% sucrose diet to determ ... | 1987 | 3478643 |
| [radiotherapy combined with brm (biological response modifiers) in the treatment of cancer]. | the latest treatment of biological response modifiers (brm) with or without another cancer treatment modalities has been increased. in this paper, radiotherapy combined with some brm, which were used in systemic administration and intra-arterial infusion, was demonstrated. 1) in experimental study, radiation and il-2 indicated the synergic effects on the tumor growth inhibition. 2) radiotherapy and ifn-beta for advanced head and neck and pelvic tumor were successful in tumor effects and tolerabl ... | 1987 | 3499528 |
| absorption kinetics of orally administered leucovorin calcium. | oral dose proportionality and pharmacokinetics of leucovorin [(d,l)-5-formyltetrahydrofolate (5-formyl-thf)] were studied in 30 healthy male subjects. in a randomized cross-over design, 24 fasted subjects were given 4 of a series of 5 single test doses between 20 and 100 mg, at 1-week intervals, of 5-formyl-thf as an oral solution of leucovorin calcium. six separate subjects received 200 mg iv and po in a 2-way crossover. blood and urine samples were collected over 24 hours for differential micr ... | 1987 | 3501546 |
| nucleotide sequence and characteristics of the gene for l-lactate dehydrogenase of thermus caldophilus gk24 and the deduced amino-acid sequence of the enzyme. | the gene for l-lactate dehydrogenase (ldh) (ec 1.1.1.27) of thermus caldophilus gk24 was cloned in escherichia coli using synthetic oligonucleotides as hybridization probes. the nucleotide sequence of the cloned dna was determined. the primary structure of the ldh was deduced from the nucleotide sequence. the deduced amino acid sequence agreed with the nh2-terminal and cooh-terminal sequences previously reported and the determined amino acid sequences of the peptides obtained from trypsin-digest ... | 1986 | 3533539 |
| design of dihydrofolate reductase inhibitors from x-ray crystal structures. | dihydrofolate reductase (dhfr) is an important therapeutic target for treatment of cancer and microbial disease. its species specificity has resulted in the sequencing of a number of vertebrate and bacterial dhfrs, and the three-dimensional structure of isozymes from escherichia coli, lactobacillus casei, and chicken liver has been elucidated, in the presence of the coenzyme nadph and of a number of inhibitors. this information has enabled scientists to try to design improved and more selective ... | 1986 | 3533642 |
| folate transport in lactobacillus salivarius. characterization of the transport mechanism and purification and properties of the binding component. | lactobacillus salivarius cells contain an inducible transport system for folate. influx via this system is time- and temperature-dependent, requires glucose and glutamine for optimum activity, and is half-maximal at folate concentrations in the nanomolar range. the folate internalized after 30 min at 30 degrees c is not released from the cells by excess extracellular folate and is recovered in cell extracts primarily in metabolized forms. a membrane-associated folate-binding protein is also pres ... | 1987 | 3584111 |
| folate analogues. 30. synthesis and biological evaluation of n10-propargyl-5,8-dideaza-5,6,7,8-tetrahydrofolic acid and related compounds. | the 5,6,7,8-tetrahydro derivative (1) of the powerful thymidylate synthase inhibitor n10-propargyl-5,8-dideazafolic acid (pddf) has been synthesized and evaluated for its antifolate activity. a convenient method for the preparation of the key intermediate 2-amino-6-(bromomethyl)-4-hydroxy-5,6,7,8-tetrahydroquinazoline (18) is described. two closely related analogues of 1 were also synthesized and evaluated for their antifolate activity and thymidylate synthase inhibition. n10-propargyl-5,8-didea ... | 1987 | 3599032 |
| examination of strains of lactobacilli for properties that may influence bacterial interference in the urinary tract. | lactobacilli strains vary in their ability to interfere with colonization of uroepithelial cells by pathogenic organisms. bacterial size, adherence capability, competitive exclusion and inhibition of pathogenic growth were examined in selected strains of lactobacilli to assess their relative importance. we tested adherence to uroepithelial cells of 34 lactobacillus isolates originating from the urogenital tract, dairy sources and chickens, and 1 lactobacillus strain isolated from a commercially ... | 1987 | 3599250 |
| effect of perorally administered lactobacilli on macrophage activation in mice. | the effect of perorally (p.o) administered lactobacillus casei and l. bulgaricus on macrophage activation in mice was studied. l. casei and l. bulgaricus were administered p.o. to mice for 8 days. the macrophage activation was measured on days 2, 3, 5, and 8 of lactobacillus administration by using biochemical and functional criteria. we measured the release of lysosomal hydrolases, the level of a nonlysosomal enzyme, and in vitro phagocytic activity of mouse peritoneal macrophages. all the assa ... | 1986 | 3733223 |
| folate and vitamin b12 levels in an urban elderly population with chronic diseases. assessment of two laboratory folate assays: microbiologic and radioassay. | folate levels of serum and red blood cells (rbc) and vitamin b12 serum levels were investigated in 326 urban chronically ill elderly ambulatory patients and 41 healthy young control subjects. two laboratory methods were used for investigating the folate levels, the microbiologic assay (mba) with lactobacillus casei and radioassay (ra). serum and rbc samples of 326 patients were tested by the folate mba and 270 of the same samples by the ria methods. in the mba 6.8% of the patients and 12.2% of c ... | 1986 | 3734309 |
| kinetics and mechanism of interaction of 10-propargyl-5,8-dideazafolate with thymidylate synthase. | the interaction of lactobacillus casei thymidylate synthase (ts) with 10-propargyl-5,8-dideazafolate (npq) in the presence of 2'-deoxyuridylate (dump) has been investigated. after formation of a rapidly reversible dump-npq-enzyme complex, a slow isomerization occurs to provide a ternary complex that can be isolated on nitrocellulose membranes or by gel filtration. unusual features of the isolable complex are the slow rate by which it is formed (t1/2 = 0.88 h) and the slow rate at which it dissoc ... | 1986 | 3754285 |
| [enhancement of the phagocytic activity of cells of the mononuclear phagocytosing system by the peroral administration of lactobacilli]. | the phagocytic function of the peritoneal exudate cells after oral and intraperitoneal administration of lactobacillus casei subsp. casei, l. plantarum and l. fermentum was studied on mice c57bl and inbred rats cdf/f344/crl. the phagocytic function was estimated by the capacity for absorbing and excreting 14c-labeled typhoid vaccine. intraperitoneal administration of a mixture of various lactobacillus strains resulted in two-fold increase of the bacteria absorption and digestion when the lactoba ... | 1986 | 3777898 |
| anticonvulsants, folate levels, and pregnancy outcome: a prospective study. | folate levels in serum and red cells, as determined by a microbiological assay using lactobacillus casei, and plasma anticonvulsant concentrations were monitored concurrently in nonpregnant (50 subjects) and pregnant (49 pregnancies in 46 subjects) epileptic women. twenty-three (46%) nonpregnant women had subnormal serum folate levels and 4 nonpregnant women (8%) showed subnormal red cell folate levels. in pregnant women not taking folate supplements, the incidence of folate deficiency increased ... | 1987 | 3827226 |
| affinity labeling of dihydrofolate reductase with an antifolate glyoxal. | dihydrofolate reductases from different species contain several highly conserved arginines, some of which have been shown by x-ray crystallography to have their guanido groups near the p-aminobenzoyl glutamate moiety of enzyme-bound methotrexate. the orientation of one of these (arg-52) appears to be completely reversed in comparing the crystal structures of escherichia coli with lactobacillus casei enzyme (bolin, j. t., filman, d. j., matthews, d. a., hamlin, r. c., and kraut, j. (1982). j. bio ... | 1985 | 3881433 |
| limited proteolysis of the bifunctional thymidylate synthase-dihydrofolate reductase from leishmania tropica. | the structure and activity of the bifunctional thymidylate synthase-dihydrofolate reductase (ts-dhfr) from the protozoan parasite leishmania tropica were examined by limited proteolysis with five different endopeptidases. each reaction resulted in a rapid, time-dependent loss of ts activity and no effect on dhfr activity. the proteolytic products were examined by nadodso4/page; each digestion produced a fragment of apparent mr approximately 35,000, and three of the five digestions generated a fr ... | 1985 | 3903747 |
| activity of the new antifolate n10-propargyl-5,8-dideazafolate and its polyglutamates against human dihydrofolate reductase, human thymidylate synthetase, and kb cells containing different levels of dihydrofolate reductase. | the action of n10-propargyl-5,8-dideazafolate (pddf) and its gamma-polyglutamyl analogues against human thymidylate synthetase and dihydrofolate reductase was examined. pddf inhibited thymidylate synthetase in a noncompetitive fashion with respect to 5,10-methylenetetrahydrofolate and dihydrofolate reductase in a competitive fashion with respect to dihydrofolate. ki values were estimated to be 20 and 250 nm, respectively. the addition of glutamyl moieties through gamma-linkage enhanced the inhib ... | 1985 | 3917852 |
| lactate dehydrogenase from streptococcus mutans: purification, characterization, and crossed antigenicity with lactate dehydrogenases from lactobacillus casei, actinomyces viscosus, and streptococcus sanguis. | a cytoplasmic fructose-1,6-diphosphate-dependent lactate dehydrogenase (ldh; ec 1.1.1.27) from streptococcus mutans omz175 was purified to homogeneity as judged by sodium dodecyl sulfate-gel electrophoresis. the purification consisted of ammonium sulfate precipitation of the cytoplasmic fraction, deae-sephacel and blue-sepharose cl.6b chromatography, and sephacryl s200 gel permeation. the catalytic activity of the purified enzyme required the presence of fructose-1,6-diphosphate with a broad opt ... | 1985 | 3917978 |
| differential interaction of cations with the thiamine and biotin transport proteins of lactobacillus casei. | lactobacillus casei cells contain separate and specific binding proteins which mediate the cellular uptake of thiamine and biotin. in buffered salt solutions, these proteins exhibit a very high affinity for their vitamin substrate. dissociation constants (kd values) at ph 7.5 are 0.03 and 0.15 nm for thiamine and biotin, respectively. optimal binding of biotin requires the presence of cations. this cation dependence is substantial since the kd for biotin is 60-fold higher in a buffer containing ... | 1985 | 3918565 |
| structure-activity relationships among substituted n-benzoyl derivatives of phenylalanine and its analogues in a microbial antitumor prescreen iii: derivatives of p-fluoro-dl-phenylalanine. | twelve substituted benzoyl derivatives of p-fluoro-dl-phenylalanine were prepared and tested for growth-inhibitory activity in a lactobacillus casei system used as an antitumor prescreen. the 12 substituted benzoyl groups were the same as those attached to o-fluorophenylalanine and m-fluorophenylalanine studied earlier. the activity of these compounds was compared vertically among themselves and horizontally with the corresponding derivatives of o-fluorophenylalanine and of m-fluorophenylalanine ... | 1985 | 3920381 |
| presence of lactobacillus casei in saliva from children and adults using a new medium. | the aim of this study was to devise a medium for cultivation of l. casei and to compare the presence of l. casei in saliva from children and adults. the new medium was produced by omitting the sugars from rogosa sl agar medium and supplementing this medium with melezitose. although all tested strains of lactobacilli grew on the new medium, the facultatively homofermentative lactobacilli, l. casei and l. plantarum, could be identified by a typical colonial morphology. l. casei was the most common ... | 1985 | 3920751 |
| protective effect of lactobacillus casei on pseudomonas aeruginosa infection in mice. | the protective effect of heat-killed lactobacillus casei yit9018 (lc 9018) against pseudomonas aeruginosa infection in mice was compared with that of corynebacterium parvum. survival of mice after intraperitoneal (i.p.) infection with p. aeruginosa was augmented in mice that had been pretreated i.p. with lc 9018 5 days previously. similar treatment of mice with c. parvum, however, was not effective at all. moreover, mice became more susceptible to infection with p. aeruginosa after such treatmen ... | 1985 | 3921464 |
| [clinical experience in lc-9018 lactobacillus preparation]. | | 1985 | 3922316 |
| purification and characterization of the bifunctional thymidylate synthetase-dihydrofolate reductase from methotrexate-resistant leishmania tropica. | thymidylate synthetase (ts) and dihydrofolate reductase (dhfr) in leishmania tropica exist as a bifunctional protein. by use of a methotrexate-resistant strain, which overproduces the bifunctional enzyme, the protein was purified 80-fold to apparent homogeneity in two steps. the native protein has an apparent molecular weight of 110 000 and consists of two subunits with identical size and charge. available data indicate that each of the subunits possesses ts and dhfr. the ts of the bifunctional ... | 1985 | 3922404 |
| influence of ph and fluoride on properties of an oral strain of lactobacillus casei grown in continuous culture. | a freshly isolated oral strain, lactobacillus casei rb1014, was grown in continuous culture to compare the effects of ph and fluoride on growth and metabolism. the cells were grown at ph 7.0 to 3.2 in the absence of fluoride and from ph 7.0 to 5.4 with 20 mm naf. cell numbers varied from 3 x 10(9) to 30 x 10(9)/ml on blood agar during alterations in the growth ph from 7.0 to 4.27. only when the culture was stressed by lowering the ph to 3.2 were cell numbers drastically reduced. cells growing at ... | 1985 | 3922892 |
| biosynthesis of d-alanyl-lipoteichoic acid by lactobacillus casei: interchain transacylation of d-alanyl ester residues. | lipoteichoic acid (lta) from lactobacillus casei contains poly(glycerophosphate) substituted with d-alanyl ester residues. the distribution of these residues in the in vitro-synthesized polymer is uniform. esterification of lta with d-alanine may occur in one of two modes: (i) addition at random or (ii) addition at a defined locus in the poly(glycerophosphate) chain followed by redistribution of the ester residues. a time-dependent transacylation of these residues from d-[14c]alanyl-lipophilic l ... | 1985 | 3922942 |
| maximum likelihood estimation of subsequence conservation. | a statistical method is presented for comparing protein sequences by partitioning the polymers and estimating each subsegment's degree of conservation. conservation is measured as a function of the number of transitions occurring in the underlying time homogeneous markov process assumed to govern amino acid mutations. the markovian assumption also permits estimation of the ancestral sequence. partitioning and estimation are carried out via maximum likelihood. the method is contrasted with the co ... | 1985 | 3923273 |
| a nitrocellulose-filter assay for the binary complex of 5-fluorodeoxyuridylate and lactobacillus casei thymidylate synthetase. | the interaction of 5-fluorodeoxyuridylate (fdump) with thymidylate synthetase to form a binary complex has been widely reported, yet previous attempts to detect this complex by nitrocellulose filtration have failed. in contrast, a nitrocellulose-filter-binding assay utilizing [6-3h]fdump which measures the interaction of the enzyme with the nucleotide is reported. extensive washing of the nitrocellulose-filtered complex between fdump and the enzyme resulted in no loss of bound ligand. following ... | 1985 | 3923859 |
| characterization and molecular cloning of cryptic plasmids isolated from lactobacillus casei. | four small cryptic plasmids were isolated from lactobacillus casei strains, and restriction endonuclease maps of these plasmids were constructed. three of the small plasmids (plz18c, plz19e, and plz19f1; 6.4, 4.9, and 4.8 kilobase pairs, respectively) were cloned into escherichia coli k-12 by using pbr322, pacyc184, and puc8 as vectors. two of the plasmids, plz18c and plz19e, were also cloned into streptococcus sanguis by using pva1 as the vector. hybridization by using nick-translated cloned 32 ... | 1985 | 3923929 |
| coronary arteritis in mice following the systemic injection of group b lactobacillus casei cell walls in aqueous suspension. | we describe the induction of an asymmetric, focal, inflammatory coronary arteritis by a single intraperitoneal injection of group b lactobacillus casei cell wall fragments in various inbred mouse strains. this coronary arteritis resembles the arteritis which is responsible for the 1-2% fatality rate among children with mucocutaneous lymph node syndrome. coronary arteritis developed in 18 of 26 c57bl/6, 14 of 26 a/j, 7 of 15 balb/c, and 8 of 15 c3heb/fej mice injected. it also developed in 2 of 4 ... | 1985 | 3924060 |
| nucleotide sequence of the thymidylate synthetase gene (thyp3) from the bacillus subtilis phage phi 3t. | the thyp3 gene, encoding thymidylate synthetase, from the bacillus subtilis phage phi 3t has been cloned and the nucleotide sequence determined. the derived amino acid sequence indicates a subunit mr of 32 748. the primary amino acid sequence is compared with the sequences of the analogous proteins specified by escherichia coli (thya), lactobacillus casei, (thya) and phage t4 (td). extensive conservation exists in all four sequences implying a shared tertiary structure. | 1985 | 3924741 |
| prevention of urinary tract infection in rats with an indigenous lactobacillus casei strain. | our previous studies have shown that indigenous bacteria are able to block the in vitro attachment of uropathogenic bacteria to human uroepithelial cells. in the present study, we applied the concept of competitive exclusion to an animal model. a chronic urinary tract infection was established in female rats with bacteria incorporated into agar beads injected periurethrally into the urinary bladder via a no. 3 french ureteral catheter. five strains of uropathogenic organisms were used in the fir ... | 1985 | 3926645 |
| dental pulp response to bacterial cell wall material. | lipopolysaccharides (lps) from bacteroides oralis and veillonella parvula and cell wall material from lactobacillus casei were studied for their capacity to induce leukocyte migration in the dental pulp and in an implanted wound chamber. three adult monkeys were challenged using lyophilized material sealed into buccal class v cavities prepared in dentin. pulp tissue responses were observed histologically eight and 72 hours after initiation of the experiment. subjacent to cut dentinal tubules, ba ... | 1985 | 3926844 |
| nucleotide sequence of the dihydrofolate reductase gene of methotrexate-resistant lactobacillus casei. | the nucleotide sequence of the dihydrofolate reductase (dhfr) gene of a methotrexate-resistant strain of lactobacillus casei, which is the source of dhfr for nuclear magnetic resonance (nmr) studies, has been determined. the derived amino acid sequence differs from that obtained by protein sequencing by the presence of aspartic acid instead of asparagine at position 8 and proline instead of leucine at position 90. the nucleotide sequences of 320-bp 5' and 335-bp 3' flanking regions of this gene ... | 1985 | 3928445 |
| kinetic evidence for two interconvertible forms of the folate transport protein from lactobacillus casei. | lactobacillus casei cells contain a folate transport protein which exhibits a high affinity for folate. the dissociation constant (kd) for folate derived from binding parameters at the steady state (at 0 degree c) is 0.4 nm at ph 7.5 and 0.1 nm at ph 6.0. in the present study, folate binding to this protein at ph 7.5 (and 0 degree c) was shown to follow second-order kinetics and to proceed with an association constant (k+1) of 4.9 x 10(7) liter/mol per min. k+1 was not affected by preincubation ... | 1985 | 3928596 |
| evidence from chemical degradation studies for a covalent bond from 5-fluoro-2'-deoxyuridylate to n-5 of tetrahydrofolate in the ternary complex of thymidylate synthetase-5-fluoro-2'-deoxyuridylate-5,10-methylenetetrahydrofolate. | in the ternary complex of thymidylate synthetase, 5-fluoro-2'-deoxyuridylate (fdump), and 5,10-methylenetetrahydrofolate (5,10-ch2h4folate), the 5-fluorouracil moiety is covalently bound to the enzyme by a sulfide linkage from c-6 and to either n-5 or n-10 of h4folate by a methylene bridge from c-5. in an effort to establish the site by which h4folate is attached to fdump, the ternary complex was subjected to reagents that cleave the c-9, n-10 bond of folate derivatives. the complex was stable t ... | 1985 | 3928628 |
| anti-tumour effect of humoral and cellular immunities mediated by a bacterial immunopotentiator, lactobacillus casei, in mice. | administration of a mixture containing lactobacillus casei yit 9018 (lc9018) and methylcholanthrene-induced fibrosarcoma (meth a) cells into the peritoneum of syngeneic balb/c mice suppressed the tumour growth and protected the mice from tumour death. with the appearance of the anti-tumour activity, serum complement-dependent tumour cytotoxic (cdc) antibody was induced on the 5th day after the administration as a result of the adjuvant effect. the cytotoxic antibody was not found in serum on the ... | 1985 | 3930065 |
| cytotoxic factor production by kupffer cells elicited with lactobacillus casei and corynebacterium parvum. | the ability of kupffer cells, spleen macrophages, pulmonary macrophages, and peritoneal macrophages (pm) to produce cytotoxic factor (ctf) was investigated in vitro. the production of ctf by kupffer cells elicited with corynebacterium parvum (cp) or lactobacillus casei yit9018 (lc9018) was higher than that of spleen, pulmonary macrophages, or pm. in addition, oxygen radical (or) production by kupffer cells or pm was measured. the production of or by kupffer cells or pm was significantly augmente ... | 1985 | 3930066 |
| purification and properties of "n alpha-benzyloxycarbonyl amino acid urethane hydrolase iii" from lactobacillus casei & atcc 7469. | | 1985 | 3930080 |
| acute joint inflammation in mice after systemic injection of the cell wall, its peptidoglycan, and chemically defined peptidoglycan subunits from various bacteria. | the systemic injection of an aqueous suspension of cell wall or its peptidoglycan (pg)-rich sonicate derived from group a streptococcus and lactobacillus casei induced acute joint lesions in balb/c, dba/1j, (balb/c x dba/1j)f1, and c3h/he mouse strains, but not in c57bl/6, dba/2, and akr strains. cell walls and their enzymatically degraded pg fragments from other bacteria as well as the synthetic disaccharide dipeptide and lactobacillus plantarum cell wall-derived disaccharide tripeptide produce ... | 1985 | 3930403 |
| augmentation of host resistance to listeria monocytogenes infection by lactobacillus casei. | the effect of heat-killed lactobacillus casei yit 9018 (lc 9018), a potent antitumor agent as well as a potent macrophage-activating agent, on the resistance of mice to listeria monocytogenes was studied. mice pretreated intravenously (i.v.) with 0.5 mg of lc 9018 showed enhanced resistance to subsequent i.v. infection with l. monocytogenes. antigenic cross-reaction between l. monocytogenes and lc 9018 was not seen in the delayed footpad reaction in mice, and lc 9018 inhibited the growth of l. m ... | 1985 | 3930748 |
| bacterial cell wall composition, lysozyme resistance, and the induction of chronic arthritis in rats. | bacterial cell wall induced arthritis is an experimental model of chronic erosive synovitis in which arthritis is induced in rats by a single injection of an aqueous suspension of cell wall fragments from selected gram-positive bacteria. to understand better the gram-positive bacterial cell wall characteristics necessary for the induction of chronic arthritis we tested the arthritogenicity of five gram-positive bacteria which were (1) lysozyme resistant and contained a polyrhamnose peptidoglycan ... | 1985 | 3931201 |
| quantitative correlations of biological activities of dactinomycin analogs and methotrexate derivatives with van der waals volume. | the biological activities namely the ability to bind with dna base pairs and the in vitro inhibition of human lymphoblastic leukemia cells of certain c-7- and n2-substituted dactinomycin (actinomycin d, amd) analogs, and the growth inhibitory activity of a series of side chain substituted methotrexate (mtx) derivatives against l 1210 mouse leukemia cells in culture and their binding affinity for dihydrofolate reductase (dhfr) enzyme extracted from this system and l. casei are found to be signifi ... | 1985 | 3931651 |
| the establishment of reproducible, complex communities of oral bacteria in the chemostat using defined inocula. | nine commonly isolated oral bacterial populations were inoculated into a glucose-limited and a glucose-excess (amino acid-limited) chemostat maintained at a constant ph 7.0 and a mean community generation time of 13.9 h. the bacterial populations were streptococcus mutans atcc 2-27351, strep. sanguis nctc 7865, strep. mitior ef 186, actinomyces viscosus wvu 627, lactobacillus casei ac 413, neisseria sp. a1078, veillonella alkalescens atcc 17745, bacteroides intermedius t 588 and fusobacterium nu ... | 1985 | 3932293 |
| multiplicity of macrolide-lincosamide-streptogramin antibiotic resistance determinants. | bacteria can resist macrolide, lincosamide, and streptogramin (mls) antibiotics enzymically by alteration of the target site or detoxification of the antibiotic. n6-dimethylation of adenine in 23 s ribosomal rna confers resistance to m, l, and s b-type (mlsb) antibiotics. investigation, by dna annealing, of the relationship between the genes specifying this resistance mechanism from streptococcus (groups a,b,d, and h, and pneumoniae), staphylococcus aureus, bacillus licheniformis, bacteroides fr ... | 1985 | 3932312 |
| the threshold growth response of lactobacillus casei to 5-methyl-tetrahydrofolic acid: implications for folate assays. | the comparative and absolute growth response of lactobacillus casei was measured nephelometrically for time periods of 17-23 h in the microbiological assay of folic acid and 5-methyl-tetrahydrofolic acid at concentrations of 0-8 ng/10 ml basal medium at ph 6.8. at concentrations of 0-1 ng/10 ml the comparative growth response to 5-methyl-tetrahydrofolic acid was markedly depressed whereas growth was the same at 2 ng/10 ml and above. comparative growth was unaffected by the length of assay incuba ... | 1985 | 3933550 |
| binding of methotrexate to dihydrofolate reductase and its relation to protonation of the ligand. | stopped-flow spectrophotometry and stopped-flow fluorometry have been used to study the binding of methotrexate (mtx) and 3-deazamethotrexate (3-deazamtx) to dihydrofolate reductase (dhfr) isoenzymes from streptococcus faecium and from lactobacillus casei. the absorbance change and fluorescence quenching that occur when mtx binds to dhfr isoenzyme ii from s. faecium (sfdhfr ii) are both biphasic and give similar apparent rate constants for both phases. the faster phase has an apparent rate const ... | 1985 | 3933559 |
| anti-tumour activity of lactobacillus casei on lewis lung carcinoma and line-10 hepatoma in syngeneic mice and guinea pigs. | the anti-tumour activity of lactobacillus casei yit 9018 (lc 9018) on lewis lung carcinoma (3ll) in c57bl/6 mice and line-10 hepatoma in strain-2 guinea pigs was examined. intravenous injection of lc 9018 was effective for inhibition of pulmonary metastases in c57bl/6 mice after s.c. inoculation with 3ll tumours. intralesional (i.l.) injection of lc 9018 was also effective for both prolongation of the survival period and inhibition of pulmonary metastases in 3ll tumour-bearing mice. the combinat ... | 1985 | 3933816 |
| protective effect of lipoteichoic acid from lactobacillus casei and lactobacillus fermentum against pseudomonas aeruginosa in mice. | lipoteichoic acid (lta) from lactobacillus casei yit 9018 or lactobacillus fermentum yit 0159 augmented the resistance of c57bl/6 mice to infection with pseudomonas aeruginosa, but conferred no resistance to listeria monocytogenes. it is suggested that lta was unable to activate macrophages. | 1985 | 3934337 |
| quantitative structure-activity relationship of antifolate inhibition of bacteria cell cultures resistant and sensitive to methotrexate. | sets of 5-(substituted benzyl)-2,4-diaminopyrimidines and 4,6-diamino-1,2-dihydro-2,2-dimethyl-1-(3-substituted phenyl)-s-triazines as well as several other antifolates were tested as inhibitors of escherichia coli dihydrofolate reductase and e. coli cell cultures both sensitive and resistant to methotrexate. from the results quantitative structure-activity relationships (qsar) were formulated. the triazines were found to inhibit sensitive and resistant cell cultures to the same degree, but the ... | 1985 | 3934385 |
| effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of phage pl-1 on a lactobacillus casei s strain. | the effect of phenolic compounds, ethyl alcohol, and sodium metabisulphite on the lytic activity of virulent bacteriophage pl-1 on a lactobacillus casei s strain isolated from a lactic acid beverage fermentation was investigated. catechin, caffeic, and gallic acids, commercially produced red, white, and champagne tannins, ethyl alcohol, and sodium metabisulphite inhibited plaque formation. catechin, caffeic, and gallic acids were the most effective inhibitors of plaque formation. commercially su ... | 1985 | 3936610 |
| inter-chain transacylation of d-alanine ester residues of lipoteichoic acid: a unique mechanism of membrane communication. | | 1985 | 3937755 |
| protective effects of bacterial immunostimulants, ok-432 and lc 9018 on pseudomonas aeruginosa infection in tumor-bearing mice. | | 1985 | 3938451 |
| nucleic acids of species of lactobacillus. | nucleic acid composition of 17 cultures representing the type or neotype strains of 15 named lactobacillus species was studied. nucleic acid characterization of these isolates was accompanied by a comparative study of conventional phenotypic reactions. the overall guanine plus cytosine mean deoxyribonucleic acid base composition ranged from 33 to 50% and genome sizes varied between 700 and 1500 x 10(6) daltons. sporolactobacillus inulinus contained 2500 x 10(6) daltons of chromosomal deoxyribonu ... | 1985 | 3939582 |
| abnormal folate metabolism in feed-related anemia of cultured channel catfish. | "no-blood disease" is a severe, feed-related anemia of channel catfish. pathological features in advanced cases include almost total absence of circulating red blood cells, hepatic fatty change, megaloblastic arrest of hematopoiesis, and intussusceptions of the small intestine. in view of similarities to folate deficiency in man studies were made regarding the intake and metabolism of folic acid. when a bacterial culture medium, containing inorganic salts and folic acid as the sole carbon source ... | 1986 | 3945624 |
| effect of nitrous oxide inactivation of vitamin b12 on the levels of folate coenzymes in rat bone marrow, kidney, brain, and liver. | the effects of nitrous oxide inactivation of the vitamin b12-dependent enzyme, methionine synthetase, on the distribution of folic acid derivatives in rat bone marrow cells, kidney, brain, and liver were determined. methionine synthetase activity was decreased by about 90% in bone marrow cells, kidney, and brain and by 83% in liver. the proportion of 5-methyltetrahydrofolate (5-ch3-h4pteglu) in n2o-exposed rats increased from 1.4- to 1.9-fold depending on the tissue examined. this increase was a ... | 1986 | 3947060 |
| determination of mouse liver 5-methyltetrahydrofolate concentration and polyglutamate forms. | the concentration and polyglutamate status of 5-methyltetrahydrofolate in mouse liver tissue extracts has been determined by enzymatic conversion to methylenetetrahydrofolate and subsequent entrapment of this cofactor form into a ternary complex with lactobacillus casei thymidylate synthase and tritiated 5-fluorodeoxyuridylate. 5-methyltetrahydrofolate was oxidized to methylenetetrahydrofolate using the reverse reaction of methylenetetrahydrofolate reductase with menadione as the ultimate electr ... | 1985 | 3998382 |
| phosphoenolpyruvate-sugar phosphotransferase transport system of streptococcus mutans: purification of hpr and enzyme i and determination of their intracellular concentrations by rocket immunoelectrophoresis. | enzyme i and hpr, the general proteins of the phosphoenolpyruvate-sugar phosphotransferase system, play a pivotal role in the control of sugar utilization in gram-negative and gram-positive bacteria. to determine whether growth conditions could modify the rate of biosynthesis of these proteins in streptococcus mutans, we first purified to homogeneity enzyme i and hpr from s. mutans atcc 27352. using specific antibodies obtained against these proteins, we determined by rocket electrophoresis the ... | 1985 | 4066033 |
| polygammaglutamyl metabolites of folic acid in human liver. | | 1973 | 4120722 |
| glycerol teichoic acid as a common antigenic factor in lactobacilli and some other gram-positive organisms. | | 1973 | 4121097 |
| studies on group antigens of lactobacillus casei. | | 1973 | 4125074 |
| energy requirement for the formation of blender-resistant complexes in lactobacillus phage infection. | | 1973 | 4126711 |
| relative biological activities of individual streptovaricins and streptovaricin acetates. | | 1974 | 4130531 |
| measurement of serum folate levels and serum folic acid-binding protein by 3h-pga radioassay. | | 1973 | 4134494 |
| turnover of dihydrofolate reductase in rapidly dividing cells. | | 1973 | 4144053 |
| antibodies to teichoic acids in man. | | 1973 | 4146386 |
| functions of baseplate components in bacteriophage t4 infection. i. dihydrofolate reductase and dihydropteroylhexaglutamate. | | 1973 | 4147531 |
| implication of a tryptophyl residue in the active site of dihydrofolate reductase. | | 1974 | 4151741 |
| conformational changes induced in dihydrofolate reductase by folates, pyridine nucleotide coenzymes, and methotrexate. | dihydrofolate reductase (ec 1.5.1.3; 5,6,7,8-tetrahydrofolate:nadp(+) oxidoreductase) from antifolate-resistant lactobacillus casei has been isolated in pure form and examined in solution by high resolution proton magnetic resonance spectroscopy. the 220 mhz proton magnetic resonance spectrum of this small enzyme (about 15,000 daltons) consists of several distinct resonance peaks that provide a sensitive nonperturbing probe of its conformational state. comparison of catalytically active enzyme w ... | 1974 | 4154440 |
| nuclear magnetic resonance studies of the binding of substrate analogs and coenzyme to dihydrofolate reductase from lactobacillus casei. | | 1974 | 4154775 |
| folic acid metabolism in vitamin b12-deficient sheep. effects of injected methionine on liver constituents associated with folate metabolism. | 1. the effects of injected l-methionine (2g every second day for 28 days) on liver folates and other constituents of liver associated with folate metabolism were studied in vitamin b(12)-deficient ewes and their pair-fed controls receiving vitamin b(12). the dose rate of methionine used was sufficient to restore almost to normal the elevated excretion in the urine of formiminoglutamate in the deficient animals. 2. liver folates active for lactobacillus casei, streptococcus faecalis r and pedioco ... | 1974 | 4155289 |
| proceedings: nmr studies of the binding of substrate analogues to l. casei dihydrofolate reductase. | | 1974 | 4155328 |
| folic acid and vitamin b12: transport and conversion to coenzyme forms. | | 1974 | 4156820 |
| effect of serum-factors other than folate on the lactobacillus casei assay. | | 1966 | 4159152 |
| cytochemical electron microscopic localization o esterase activity in lactobacillus casei. | enzymes capable of hydrolyzing thiolacetic acid in two strains of lactobacillus casei were localized by the formation of electron-opaque pbs deposits in vivo in the presence of pb(no(3))(2). by electron microscopy, the deposition of pbs appeared primarily at the plasma membrane and at the outer surface of the cell wall. | 1970 | 4195477 |
| [proteolytic degradation of milk proteins by bacteria. 2. the action of psychrophilic and lactic acid producing bacteria on the proteins in milk]. | | 1972 | 4196274 |