| Synthesis of silver nanoparticles by solar irradiation of cell-free Bacillusamyloliquefaciens extracts and AgNO(3). | Silver nanoparticles (AgNPs) were obtained by solar irradiation of cell-free extracts of Bacillusamyloliquefaciens and AgNO(3). Light intensity, extract concentration, and NaCl addition influenced the synthesis of AgNPs. Under optimized conditions (solar intensity 70,000lx, extract concentration 3mg/mL, and NaCl content 2mM), 98.23±0.06% of the Ag(+) (1mM) was reduced to AgNPs within 80min, and the ?-potential of AgNPs reached -70.84±0.66mV. TEM (Transmission electron microscopy) and XRD (X-ray ... | 2012 | 22019398 |
| biocontrol mechanism by root-associated bacillus amyloliquefaciens fzb42 - a review. | bacillus amyloliquefaciens subsp. plantarum fzb42 is a gram-positive model bacterium for unraveling plant-microbe interactions in bacilli. in addition, fzb42 is used commercially as biofertilizer and biocontrol agent in agriculture. genome analysis of fzb42 revealed that nearly 10% of the fzb42 genome is devoted to synthesizing antimicrobial metabolites and their corresponding immunity genes. however, recent investigations in planta demonstrated that - except surfactin - the amount of such compo ... | 2015 | 26284057 |
| effect of tin oxide nanoparticle binding on the structure and activity of α-amylase from bacillus amyloliquefaciens. | proteins adsorbed on nanoparticles (nps) are being used in biotechnology, biosensors and drug delivery. however, understanding the effect of nps on the structure of proteins is still in a nascent state. in the present paper tin oxide (sno2) nps were synthesized by the reaction of sncl4·5h2o in methanol via the sol-gel method and characterized by x-ray diffraction (xrd), fourier transform infrared spectroscopy (ft-ir) and transmission electron microscopy (tem). the binding of these sno2-nps with ... | 2011 | 22020314 |
| distinct differentiation of closely related species of bacillus subtilis group with industrial importance. | pcr amplification of 16s rrna gene by universal primers followed by restriction fragment length polymorphism analysis using rsai, cfoi and hinfi endonucleases, distinctly differentiated closely related bacillus amyloliquefaciens, bacillus licheniformis and bacillus pumilus from bacillus subtilis sensu stricto. this simple, economical, rapid and reliable protocol could be an alternative to misleading phenotype-based grouping of these closely related species. | 2011 | 21889958 |
| comparative structural bioinformatics analysis of bacillus amyloliquefaciens chemotaxis proteins within bacillus subtilis group. | chemotaxis is a process in which bacteria sense their chemical environment and move towards more favorable conditions. since plant colonization by bacteria is a multifaceted process which requires a response to the complex chemical environment, a finely tuned and sensitive chemotaxis system is needed. members of the bacillus subtilis group including bacillus amyloliquefaciens are industrially important, for example, as bio-pesticides. the group exhibits plant growth-promoting characteristics, wi ... | 2011 | 21993479 |
| biodegradation of soil-applied pesticides by selected strains of plant growth-promoting rhizobacteria (pgpr) and their effects on bacterial growth. | a laboratory study was conducted to investigate the influence of four pgpr strains on the degradation of five soil applied pesticides and their effects on bacterial growth. interactions of bacillus subtilis gb03, bacillus subtilis fzb24, bacillus amyloliquefaciens in937a and bacillus pumilus se34 with two concentrations of acibenzolar-s-methyl, metribuzin, napropamide, propamocarb hydrochloride and thiamethoxam in liquid culture and soil microcosm were studied. the degradation of acibenzolar-s-m ... | 2011 | 21870159 |
| purification and characterization of levansucrases from bacillus amyloliquefaciens in intra- and extracellular forms useful for the synthesis of levan and fructooligosaccharides. | the intra- and extracellular levansucrase (ls) activities produced by bacillus amyloliquefaciens were promoted by supplementing the sucrose medium with yeast and peptone as nitrogen sources. these activities were purified by polyethylene glycol (peg) fractionation for the first time. pegs of low molecular weight selectively fractionated the intracellular ls activity rather than the extracellular ls activity. contrary to other lss, b. amyloliquefaciens lss exhibited high levan-forming activity ov ... | 2011 | 21979064 |
| novel routes for improving biocontrol activity of bacillus based bioinoculants. | biocontrol (bc) formulations prepared from plant-growth-promoting bacteria are increasingly applied in sustainable agriculture. especially inoculants prepared from endospore-forming bacillus strains have been proven as efficient and environmental-friendly alternative to chemical pesticides due to their long shelf life, which is comparable with that of agrochemicals. however, these formulations of the first generation are sometimes hampered in their action and do not fulfill in each case the expe ... | 2015 | 26696998 |
| Improved thermostable a-amylase activity of Bacillus amyloliquefaciens by low-energy ion implantation. | Thermostable a-amylase is of great importance in the starch fermentation industry; it is extensively used in the manufacture of beverages, baby foods, medicines, and pharmaceuticals. Bacillus amyloliquefaciens produces thermostable a-amylase; however, production of thermostable a-amylase is limited. Ion-beam implantation is an effective method for mutation breeding in microbes. We conducted ion-beam implantation experiments using two different ions, Ar(+) and N(+), to determine the survi ... | 2011 | 21968725 |
| The species-specific mode of action of the antimicrobial peptide subtilosin against Listeria monocytogenes Scott A. | Aims: To elucidate the molecular mechanism of action of the antimicrobial peptide subtilosin against the foodborne pathogen Listeria monocytogenes Scott A. Methods and Results: Subtilosin was purified from a culture of Bacillus amyloliquefaciens. The minimal inhibitory concentration of subtilosin against L. monocytogenes Scott A was determined by broth microdilution method. The effect of subtilosin on the transmembrane electrical potential (??) and pH gradient (?pH), and its ability to induce ... | 2012 | 22040458 |
| bacillus oryzicola sp. nov., an endophytic bacterium isolated from the roots of rice with antimicrobial, plant growth promoting, and systemic resistance inducing activities in rice. | biological control of major rice diseases has been attempted in several rice-growing countries in asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. two novel endophytic bacillus species, designated strains yc7007 and yc7010(t), with anti-microbial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at ji ... | 2015 | 26060434 |
| response of tomato wilt pathogen ralstonia solanacearum to the volatile organic compounds produced by a biocontrol strain bacillus amyloliquefaciens sqr-9. | it is important to study the response of plant pathogens to the antibiosis traits of biocontrol microbes to design the efficient biocontrol strategies. in this study, we evaluated the role of volatile organic compounds (vocs) produced by a biocontrol strain bacillus amyloliquefaciens sqr-9 on the growth and virulence traits of tomato wilt pathogen ralstonia solanacearum (rs). the vocs of sqr-9 significantly inhibited the growth of rs on agar medium and in soil. in addition, the vocs significantl ... | 2016 | 27103342 |
| Quantification of the antifungal lipopeptide iturin A by high performance liquid chromatography coupled with aqueous two-phase extraction. | Iturin A, a powerful antifungal surfactant, is a kind of bacterial lipopeptide produced by Bacillus strains. This study addresses the use of an aqueous two-phase system (ATPS) using ethanol/ammonium sulfate to extract iturin A from Bacillus amyloliquefaciens NJN-6 fermentation broth and the quantification of iturin A by HPLC. Baseline separation of iturin A homologues was performed using an RP-C(18) column with a mixture of water and acetonitrile. The results showed that the correlation coeffici ... | 2011 | 21872538 |
| bacillus volatiles adversely affect the physiology and ultra-structure of ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt. | volatile organic compounds (vocs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. six of the most effective antagonistic bacillus spp. were used in this study against ralstonia solanacearum (rsc) tbbs1, the causal agent of bacterial wilt disease in tobacco. bacillus amyloliquefaciens fzb42 and bacillus artrophaeus lssc22 had the strongest inhibitory effect against rsc. thirteen vocs produced by fzb42 and 10 by lssc22 were identified ... | 2017 | 28091587 |
| An analysis of temperature adaptation in cold active, mesophilic and thermophilic Bacillus a-amylases. | A comparative biochemical and structural study was performed on a cold active a-amylase from Bacillus cereus (BCA) and two well-known homologous mesophilic and thermophilic a-amylases from Bacillus amyloliquefaciens (BAA) and Bacillus licheniformis (BLA). In spite of a high degree of sequence and structural similarity, drastic variations were found for T(opt) as 50, 70 and 90°C for BCA, BAA and BLA, respectively. The half-lives of thermoinactivation were 1 and 9 min for BCA and BAA at 80°C respe ... | 2011 | 21907234 |
| [isolation and identification of a bacillus amyloliquefaciens yb-3 against rhizoctonia solani]. | an antagonistic bacterial strain yb-3 against rhizoctonia solani was isolated from soils. | 2011 | 22097779 |
| Elucidation of the Molecular Mechanisms of Action of the Natural Antimicrobial Peptide Subtilosin Against the Bacterial Vaginosis-associated Pathogen Gardnerella vaginalis. | Subtilosin A is a 35-amino acid long cyclical peptide produced by Bacillus amyloliquefaciens that has potent antimicrobial activity against a variety of human pathogens, including the bacterial vaginosis-related Gardnerella vaginalis. The specific mode of action of subtilosin against G. vaginalis was elucidated by studying its effects on the proton motive force's (PMF) components: transmembrane electric potential (??), transmembrane pH gradient (?pH), and intracellular ATP levels. The addition o ... | 2011 | 21949544 |
| Purification and Characterization of a Major Fibrinolytic Enzyme from Bacillus amyloliquefaciens MJ5-41 Isolated from Meju. | Meju is a traditional Korean fermented soy product used as a key element for soy sauce and doenjang. Bacilli with antimicrobial activity were isolated from meju prepared by traditional methods at Sunchang county, Jeollabukdo, Korea. Six isolates were identified as Bacillus amyloliquefaciens by recA gene sequencing and RAPD-PCR. One isolate, B. amyloliquefaciens MJ5-41, showed the strongest fibrinolytic activity. A 27 kDa active fibrinolytic enzyme, AprE5-41, was purified from the culture superna ... | 2011 | 22127128 |
| the antibacterial activity and mechanism of ginkgolic acid c15:1. | the present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (ga) c15:1 monomer using green fluorescent protein (gfp)-labeled bacteria strains. | 2017 | 28088196 |
| isolation and identification of phyllospheric bacteria possessing antimicrobial activity from astragalus obtusifolius, prosopis juliflora, xanthium strumarium and hippocrepis unisiliqousa. | the widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products. | 2017 | 28090278 |
| The plant-associated Bacillus amyloliquefaciens strains MEP(2) 18 and ARP(2) 3 capable of producing the cyclic lipopeptides iturin or surfactin and fengycin are effective in biocontrol of sclerotinia stem rot disease. | Aims: This work was conducted to identify the antifungal compounds produced by two previously isolated Bacillus sp. strains: ARP(2) 3 and MEP(2) 18. Both strains were subjected to further analysis to determine their taxonomic position and to identify the compounds responsible for their antifungal activity as well as to evaluate the efficiency of these strains to control sclerotinia stem rot in soybean. Methods and Results: The antifungal compounds were isolated by acid precipitation of cell-fr ... | 2012 | 22017648 |
| bacillus amyloliquefaciens g1: a potential antagonistic bacterium against eel-pathogenic aeromonas hydrophila. | recent studies have revealed that the use of probiotics is an alternative to control marine aeromonas. however, few probiotics are available against aeromonas hydrophila infections in eels. in the present study, a potential antagonistic strain g1 against the eel-pathogenic a. hydrophila was isolated from sediment underlying brackish water. its extracellular products with antibacterial activities were shown to be stable under wide range of ph, temperature, and proteinase k. it was initially ident ... | 2011 | 21754944 |
| construction and characterization of a fusion +¦-1,3-1,4-glucanase to improve hydrolytic activity and thermostability. | a new fusion gene (bgl-licmb), encoding +¦-1,3-1,4-glucanase both from bacillus amyloliquefaciens (bgl) and clostridium thermocellum (licmb), was constructed via end-to-end fusion and expressed in escherichia coli to improve hydrolytic activity and thermostability of +¦-1,3-1,4-glucanase. the results of enzymatic properties showed that the catalytic efficiency (k(cat)/k(m)) of the fusion enzyme for oat +¦-glucan was 2.7 and 20-fold higher than that of the parental bgl and licmb, respectively, an ... | 2011 | 21735259 |
| complete genome sequence of bacillus amyloliquefaciens strain co1-6, a plant growth-promoting rhizobacterium of calendula officinalis. | the genome sequence of bacillus amyloliquefaciens strain co1-6, a plant growth-promoting rhizobacterium (pgpr) with broad-spectrum antagonistic activity against plant-pathogenic fungi, bacteria, and nematodes, consists of a single 3.9-mb circular chromosome. the genome reveals genes putatively responsible for its promising biocontrol and pgp properties. | 2015 | 26272562 |
| pectin enhances bio-control efficacy by inducing colonization and secretion of secondary metabolites by bacillus amyloliquefaciens sqy 162 in the rhizosphere of tobacco. | bacillus amyloliquefaciens is a plant-beneficial gram-positive bacterium involved in suppressing soil-borne pathogens through the secretion of secondary metabolites and high rhizosphere competence. biofilm formation is regarded as a prerequisite for high rhizosphere competence. in this work, we show that plant extracts affect the chemotaxis and biofilm formation of b. amyloliquefaciens sqy 162 (sqy 162). all carbohydrates tested induced the chemotaxis and biofilm formation of the sqy 162 strain; ... | 2015 | 25996156 |
| antibacterial compounds-macrolactin alters the soil bacterial community and abundance of the gene encoding pks. | macrolactin produced by many soil microbes has been shown to be an efficient antibacterial agent against many bacterial pathogens. however, studies examining the effect of macrolactin on both the soil bacterial community and the intrinsic bacterial species that harbor genes responsible for the production of this antibiotic have not been conducted so far. in this study, a mixture of macrolactin was isolated from the liquid culture of bacillus amyloliquefaciens njn-6, and applied to the soil once ... | 2016 | 27965639 |
| biological control activities of rice-associated bacillus sp. strains against sheath blight and bacterial panicle blight of rice. | potential biological control agents for two major rice diseases, sheath blight and bacterial panicle blight, were isolated from rice plants in this study. rice-associated bacteria (rabs) isolated from rice plants grown in the field were tested for their antagonistic activities against the rice pathogens, rhizoctonia solani and burkholderia glumae, which cause sheath blight and bacterial panicle blight, respectively. twenty-nine rabs were initially screened based on their antagonistic activities ... | 2016 | 26765124 |
| barnase and binase: twins with distinct fates. | rnases are enzymes that cleave rnas, resulting in remarkably diverse biological consequences. many rnases are cytotoxic. in some cases, they attack selectively malignant cells triggering an apoptotic response. a number of eukaryotic and bacterial rnase-based strategies are being developed for use in anticancer and antiviral therapy. however, the physiological functions of these rnases are often poorly understood. this review focuses on the properties of the extracellular rnases from bacillusôçâa ... | 2011 | 21824291 |
| the impact of the pathogen rhizoctonia solani and its beneficial counterpart bacillus amyloliquefaciens on the indigenous lettuce microbiome. | lettuce belongs to the most commonly raw eaten food worldwide and its microbiome plays an important role for both human and plant health. yet, little is known about the impact of potentially occurring pathogens and beneficial inoculants of the indigenous microorganisms associated with lettuce. to address this question we studied the impact of the phytopathogenic fungus rhizoctonia solani and the biological control agent bacillus amyloliquefaciens fzb42 on the indigenous rhizosphere and phyllosph ... | 2014 | 24795707 |
| drna-seq reveals genomewide tsss and noncoding rnas of plant beneficial rhizobacterium bacillus amyloliquefaciens fzb42. | bacillus amyloliquefaciens subsp. plantarum fzb42 is a representative of gram-positive plant-growth-promoting rhizobacteria (pgpr) that inhabit plant root environments. in order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain fzb42 grown under rhizosphere-mimicking conditions using differential rna sequencing (drna-seq). our analysis revealed 4,877 transcription start sites for protein-coding genes, id ... | 2015 | 26540162 |
| difficidin and bacilysin from bacillus amyloliquefaciens fzb42 have antibacterial activity against xanthomonas oryzae rice pathogens. | bacterial blight and bacterial leaf streak are serious, economically damaging, diseases of rice caused by the bacteria xanthomonas oryzae pv. oryzae and x. oryzae pv. oryzicola. bacillus amyloliquefaciens fzb42 was shown to possess biocontrol activity against these xanthomonas strains by producing the antibiotic compounds difficidin and bacilysin. analyses using fluorescence, scanning electron and transmission electron microscopy revealed difficidin and bacilysin caused changes in the cell wall ... | 2015 | 26268540 |
| Structure determination and interception of biosynthetic intermediates for the plantazolicin class of highly discriminating antibiotics. | The soil-dwelling, plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42 is a prolific producer of complex natural products. Recently, a new FZB42 metabolite, plantazolicin (PZN), has been described as a member of the growing thiazole/oxazole-modified microcin (TOMM) family. TOMMs are biosynthesized from inactive, ribosomal peptides and undergo a series of cyclodehydrations, dehydrogenations, and other modifications to become bioactive natural products. Using high-resolution mass spe ... | 2011 | 21950656 |
| secondary metabolites from bacillus amyloliquefaciens isolated from soil can kill burkholderia pseudomallei. | bacillus species are gram-positive bacteria found in abundance in nature and their secondary metabolites were found to possess various potential activities, notably antimicrobial. in this study, bacillus amyloliquefaciens n2-4 and n3-8 were isolated from soil and their metabolites could kill burkholderia pseudomallei, a gram-negative pathogenic bacterium also found in soil in its endemic areas. moreover, the metabolites were able to kill drug resistant isolates of b. pseudomallei and also inhibi ... | 2017 | 28050857 |
| glycin-rich antimicrobial peptide yd1 from b. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid dna of e. coli. | antimicrobial peptides (amps), low-molecular-weight proteins with broad-spectrum antimicrobial activity, are the most promising candidates for the development of novel antimicrobials. a powerful cationic glycine-rich amp yd1 (mw ~ 1.0 kda) was purified from bacillus amyloliquefaciens cbsyd1 isolated from traditional korean fermented food kimchi, for the treatment of multidrug-resistant (mdr) bacteria. strain cbsyd1 was identified 99.79% similar to bacillus amyloliquefaciens subsp. plantarum fzb4 ... | 2017 | 28050849 |
| Antimicrobial factor from Bacillus amyloliquefaciens inhibits Paenibacillus larvae, the causative agent of American foulbrood. | Bacillus amyloliquefaciens LBM 5006 produces an antimicrobial factor active against Paenibacillus larvae, a major honeybee pathogen. The antagonistic effect and the mode of action of the antimicrobial factor were investigated. The antibacterial activity was produced starting at mid-logarithmic growth phase, reaching its maximum during the stationary phase. Exposure of cell suspensions of P. larvae to this antimicrobial resulted in loss of cell viability and reduction in optical density associate ... | 2011 | 21858429 |
| evidence regarding the hypothesis that the histidine-histidine contact pairs may affect protein stability. | it has been lately proposed that the interaction between like-charged residues stabilizes the native state of proteins. to explore this, we created a histidine-histidine pair in the ca-iii binding site of the bacillus amyloliquefaciens α-amylase (baa) and then examined the impact of this pairing on the baa. for this purpose, we used site-directed mutagenesis (sdm) to substitute pro407 with his, ala, gln, arg, and glu in the baa. subsequently, thermostability, kinetic parameters and structural pr ... | 2012 | 22257446 |
| microbial inoculants for the biocontrol of fusarium spp. in durum wheat. | fusarium head blight (fhb) is a severe disease caused by different fusarium species, which affects a wide range of cereal crops, including wheat. it determines from 10 to 30% of yield loss in europe. chemical fungicides are mainly used to reduce the incidence of fhb, but low environmental impact solutions are looked forward. applications of soil/rhizobacteria as biocontrol agents against fhb in wheat are described in literature, whereas the potential use of lactobacilli in agriculture has scarce ... | 2015 | 26518441 |
| bacilysin from bacillus amyloliquefaciens fzb42 has specific bactericidal activity against harmful algal bloom species. | harmful algal blooms, caused by massive and exceptional overgrowth of microalgae and cyanobacteria, are a serious environmental problem worldwide : in the present study, we looked for bacillus strains with sufficiently strong anticyanobacterial activity to be used as biocontrol agents. among 24 strains, bacillus amyloliquefaciens fzb42 showed the strongest bactericidal activity against microcystis aeruginosa, with a kill rate of 98.78%. the synthesis of the anticyanobacterial substance did not d ... | 2014 | 25261512 |
| deciphering the conserved genetic loci implicated in plant disease control through comparative genomics of bacillus amyloliquefaciens subsp. plantarum. | to understand the growth-promoting and disease-inhibiting activities of plant growth-promoting rhizobacteria (pgpr) strains, the genomes of 12 bacillus subtilis group strains with pgpr activity were sequenced and analyzed. these b. subtilis strains exhibited high genomic diversity, whereas the genomes of b. amyloliquefaciens strains (a member of the b. subtilis group) are highly conserved. a pairwise blastp matrix revealed that gene family similarity among bacillus genomes ranges from 32 to 90%, ... | 2015 | 26347755 |
| culturable bacterial endophytes isolated from mangrove tree (rhizophora apiculata blume) enhance seedling growth in rice. | endophytic bacteria do have several potential applications in medicine and in other various sectors of biotechnology including agriculture. bacterial endophytes need to be explored for their potential applications in agricultural biotechnology. one of the potential applications of bacterial endophytes in agricultural is to enhance the growth of the agricultural crops. hence, this study was undertaken to explore the plant growth promoting potential application of bacterial endophytes. | 2014 | 25097431 |
| amylocyclicin, a novel circular bacteriocin produced by bacillus amyloliquefaciens fzb42. | bacillus amyloliquefaciens fzb42 is a gram-positive plant growth-promoting bacterium with an impressive capacity to synthesize nonribosomal secondary metabolites with antimicrobial activity. here we report on a novel circular bacteriocin which is ribosomally synthesized by fzb42. the compound displayed high antibacterial activity against closely related gram-positive bacteria. transposon mutagenesis and subsequent site-specific mutagenesis combined with matrix-assisted laser desorption ionizatio ... | 2014 | 24610713 |
| mosquitocidal bacillus amyloliquefaciens: dynamics of growth & production of novel pupicidal biosurfactant. | a strain of bacillus amyloliquefaciens (vcrc b483) producing mosquito larvicidal and pupicidal biosurfactant was isolated from mangrove forest soil. the present study was aimed at studying the kinetics of growth and production of the mosquitocidal biosurfactant by this bacterium. | 0 | 25366212 |
| immune tolerance to an intestine-adapted bacteria, chryseobacterium sp., injected into the hemocoel of protaetia brevitarsis seulensis. | to explore the interaction of gut microbes and the host immune system, bacteria were isolated from the gut of protaetia brevitarsis seulensis larvae. chryseobacterium sp., bacillus subtilis, arthrobacter arilaitensis, bacillus amyloliquefaciens, bacillus megaterium, and lysinibacillus xylanilyticus were cultured in vitro, identified, and injected in the hemocoel of p. brevitarsis seulensis larvae, respectively. there were no significant changes in phagocytosis-associated lysosomal formation or p ... | 2016 | 27530146 |
| response surface methodology optimization of fermentation conditions for rapid and efficient accumulation of macrolactin a by marine bacillus amyloliquefaciens esb-2. | in the present work, an antibiotic-producing marine bacterium was isolated from a seawater sample collected from yuhuan, zhejiang, china, identified and named as bacillus amyloliquefaciens esb-2 on the basis of phenotypic characteristics and 16s rrna gene sequencing. response surface methodology was applied to optimize the fermentation conditions for rapid and efficient accumulation of macrolactin a, a pharmacologically important marine antibiotic. eight fermentation conditions were examined for ... | 2012 | 23275049 |
| production of the α-glycosidase inhibitor 1-deoxynojirimycin from bacillus species. | 1-deoxynojirimycin (dnj), a potent α-glycosidase inhibitor, has therapeutic applications in treatments of hiv, gaucher's disease, and diabetes. dnj has been extracted from natural sources (mulberry leaves) for therapeutic purposes; however, dnj ingredients are in limited supply and are costly to obtain on a large scale. since certain strains of bacillus and streptomyces species reportedly produce dnj, they may serve as potential sources for high-yield dnj production. in this study, we obtained e ... | 2012 | 23265519 |
| developmentally-regulated excision of the spβ prophage reconstitutes a gene required for spore envelope maturation in bacillus subtilis. | temperate phages infect bacteria by injecting their dna into bacterial cells, where it becomes incorporated into the host genome as a prophage. in the genome of bacillus subtilis 168, an active prophage, spβ, is inserted into a polysaccharide synthesis gene, spsm. here, we show that a rearrangement occurs during sporulation to reconstitute a functional composite spsm gene by precise excision of spβ from the chromosome. spβ excision requires a putative site-specific recombinase, spra, and an acce ... | 2014 | 25299644 |
| the antibacterial activity of e. coli bacteriophage lysin lysep3 is enhanced by fusing the bacillus amyloliquefaciens bacteriophage endolysin binding domain d8 to the c-terminal region. | bacteriophage endolysin is one of the most promising antibiotic substitutes, but in gram-negative bacteria, the outer membrane prevents the lysin from hydrolyzing peptidoglycans and blocks the development of lysin applications. the prime strategy for new antibiotic substitutes is allowing lysin to access the peptidoglycan from outside of the bacteria by reformation of the lysin. in this study, the novel escherichia coli (e. coli) phage lyase lysep3, which lacks outside-in catalytic ability, was ... | 2017 | 28124780 |
| antimicrobial resistance and plasmid profile of bacterial strains isolated from the urbanized eltsovka-1 river (russia). | antimicrobial resistance and plasmid profile of gram-positive and gram-negative bacterial strains isolated from the urbanized eltsovka-1 river (russia) were investigated. sequencing of the 16s rrna of of g+ strains showed 99-100% identity to that of bacillus aerophilus, bacillus altitudinis, bacillus amyloliquefaciens, bacillus anthrancis, bacillus barbaricus, bacillus cereus, bacillus flexus, bacillus indriensis, bacillus stratosphericus, bacillus subtilis subsp. subtilis, bacillus thuringiensi ... | 2015 | 25803299 |
| evaluation and selection of bacillus species based on enzyme production, antimicrobial activity, and biofilm synthesis as direct-fed microbial candidates for poultry. | social concern about misuse of antibiotics as growth promoters (agp) and generation of multidrug-resistant bacteria have restricted the dietary inclusion of antibiotics in livestock feed in several countries. direct-fed microbials (dfm) are one of the multiple alternatives commonly evaluated as substitutes of agp. sporeformer bacteria from the genus bacillus have been extensively investigated because of their extraordinary properties to form highly resistant endospores, produce antimicrobial com ... | 2016 | 27812526 |
| effects of potential probiotic bacillus amyloliquefaciens [corrected] fptb16 on systemic and cutaneous mucosal immune responses and disease resistance of catla (catla catla). | effects of dietary administration of bacillus amyloliquefaciens fptb16 on systemic and mucosal immunity and disease resistance of catla (catla catla) against edwardsiella tarda infection were evaluated in the present study. the laboratory maintained b. amyloliquefaciens was used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. healthy catla were challenged by this bacterium for determination of its safety. for preparation of probiotic supplemented die ... | 2013 | 24012512 |
| characterization of a mannose-6-phosphate isomerase from bacillus amyloliquefaciens and its application in fructose-6-phosphate production. | the bam6pi gene encoding a mannose-6-phosphate isomerase (m6pi, ec 5.3.1.8) was cloned from bacillus amyloliquefaciens dsm7 and overexpressed in escherichia coli. the enzyme activity of bam6pi was optimal at ph and temperature of 7.5 and 70°c, respectively, with a kcat/km of 13,900 s-1 mm-1 for mannose-6-phosphate (m6p). the purified bam6pi demonstrated the highest catalytic efficiency of all characterized m6pis. although m6pis have been characterized from several other sources, bam6pi is distin ... | 2015 | 26171785 |
| acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in bacillus amyloliquefaciens. | lysine acetylation is a major post-translational modification that plays an important regulatory role in almost every aspects in both eukaryotes and prokaryotes. bacillus amyloliquefaciens, a gram-positive bacterium, is very effective for the control of plant pathogens. however, very little is known about the function of lysine acetylation in this organism. here, we conducted the first lysine acetylome in b. amyloliquefaciens through a combination of highly sensitive immune-affinity purification ... | 2016 | 26822828 |
| ex vivo application of secreted metabolites produced by soil-inhabiting bacillus spp. efficiently controls foliar diseases caused by alternaria spp. | bacterial biological control agents (bcas) are largely used as live products to control plant pathogens. however, due to variable environmental and ecological factors, live bcas usually fail to produce desirable results against foliar pathogens. in this study, we investigated the potential of cell-free culture filtrates of 12 different bacterial bcas isolated from flower beds for controlling foliar diseases caused by alternaria spp. in vitro studies showed that culture filtrates from two isolate ... | 2015 | 26519395 |
| biocontrol and plant growth promotion characterization of bacillus species isolated from calendula officinalis rhizosphere. | the phenotypic and genotypic diversity of the plant growth promoting bacillus genus have been widely investigated in the rhizosphere of various agricultural crops. however, to our knowledge this is the first report on the bacillus species isolated from the rhizosphere of calendula officinalis. 15 % of the isolated bacteria were screened for their important antifungal activity against fusarium oxysporum, botrytis cinerea, aspergillus niger, cladosporium cucumerinium and alternaria alternata. the ... | 2013 | 24426149 |
| analyzing the relation between the microbial diversity of daqu and the turbidity spoilage of traditional chinese vinegar. | vinegar is a traditional fermented condiment, and the microbial diversity of daqu makes the quality of vinegar products. recently, turbidity spoilage of vinegar sharply tampered with the quality of vinegar. in this study, the relation between the microbial diversity of daqu and turbidity spoilage of vinegar was analyzed by plating technique, pcr-denaturing gradient gel electrophoresis (dgge), and high-performance liquid chromatography (hplc). the 16s rrna sequencing and dgge analysis indicated t ... | 2014 | 24691870 |
| diversity of food-borne bacillus volatile compounds and influence on fungal growth. | to evaluate the antifungal activity of the volatile organic compounds (vocs) produced by 75 different food-borne bacillus species against aspergillus niger, aspergillus flavus, aspergillus parasiticus, aspergillus clavatus, fusarium oxysporum f. sp. lactucae and moniliophthora perniciosa and to determine the vocs responsible for the inhibition. | 2015 | 25989039 |
| in-vitro antibacterial, antifungal, antioxidant and functional properties of bacillus amyloliquefaciens. | food born pathogenic bacteria and filamentous fungi are able to grow on most foods, including natural foods, processed foods, and fermented foods and create considerable economic loss. the aim of this study was to determine the antibacterial, antifungal, antioxidant and functional properties of bacillus amyloliquefaciens recovered from silage. | 2015 | 25858278 |
| biological control as a strategy to reduce the impact of mycotoxins in peanuts, grapes and cereals in argentina. | mycotoxins including aflatoxins, deoxynivalenol, fumonisins and ochratoxin a are among the main fungal secondary metabolites detected as natural contaminants in south america in different commodities such as peanuts (aflatoxins), cereals (deoxynivalenol and fumonisins) or grapes (ochratoxin a). different strategies including crop rotation, tillage practices, fungicide application and planting less susceptible cultivars are used in order to reduce the impact of these mycotoxins in both food and f ... | 2015 | 25427716 |
| influence of inoculation with plant growth promoting rhizobacteria (pgpr) on tomato plant growth and nematode reproduction under greenhouse conditions. | numerous species of soil bacteria which flourish in the rhizosphere of plants or around plant tissues stimulate plant growth and reduce nematode population by antagonistic behavior. these bacteria are collectively known as pgpr (plant growth promoting rhizobacteria). the effects of six isolates of pgpr pseudomonas putida, pseudomonas fluorescens, serratia marcescens, bacillus amyloliquefaciens, bacillus subtilis and bacillus cereus, were studied on tomato plant growth and root knot nematode repr ... | 2012 | 23961220 |
| extracts containing clps of bacillus amyloliquefaciens jn68 isolated from chicken intestines exert antimicrobial effects, particularly on methicillin-resistant staphylococcus aureus and listeria monocytogenes. | bacillus amyloliquefaciens jn68, which has been discussed with regards to its antimicrobial activities, was successfully isolated from healthy chicken intestines in the present study. using the spot-on-the-lawn antagonism method, the preliminary study indicated that a suspension culture of the b. amyloliquefaciens jn68 strain can inhibit the growth of aspergillus niger and penicillium pinophilum. furthermore, the cyclic lipopeptides (clps) produced by the b. amyloliquefaciens jn68 strain were fu ... | 2016 | 27840979 |
| responses of beneficial bacillus amyloliquefaciens sqr9 to different soilborne fungal pathogens through the alteration of antifungal compounds production. | bacillus amyloliquefaciens sqr9 exhibited predominantly antagonistic activities against a broad range of soilborne pathogens. the fungi-induced sqr9 extracts possess stronger antifungal activities compared with sqr9 monoculture extracts. to investigate how sqr9 fine-tunes lipopeptides (lps) and a siderophore bacillibactin production to control different fungal pathogens, lps and bacillibactin production and transcription of the respective encoding genes in sqr9 were measured and compared with si ... | 2014 | 25484880 |
| transposon mutagenesis of the plant-associated bacillus amyloliquefaciens ssp. plantarum fzb42 revealed that the nfra and rbam17410 genes are involved in plant-microbe-interactions. | bacillus amyloliquefaciens ssp. plantarum fzb42 represents the prototype of gram-positive plant growth promoting and biocontrol bacteria. in this study, we applied transposon mutagenesis to generate a transposon library, which was screened for genes involved in multicellular behavior and biofilm formation on roots as a prerequisite of plant growth promoting activity. transposon insertion sites were determined by rescue-cloning followed by dna sequencing. as in b. subtilis, the global transcripti ... | 2014 | 24847778 |
| antifungal cyclic lipopeptides from bacillus amyloliquefaciens strain bo5a. | a bioassay-guided fractionation of bacillus amyloliquefaciens strain bo5a afforded the isolation of two new cyclic lipopeptides (1 and 2) as the major lipid constituents (>60%) of the chcl3-meoh (2:1) extract. the chemical structures of the isolated metabolites were elucidated by spectroscopic methods, including 1d and 2d nmr spectroscopy, mass spectrometry (ms), secondary ion mass spectrometry (ms1, ms2), and chemical degradation. the compounds are members of the surfactins family and are based ... | 2013 | 24164115 |
| metabolomic profiles of aspergillus oryzae and bacillus amyloliquefaciens during rice koji fermentation. | rice koji, used early in the manufacturing process for many fermented foods, produces diverse metabolites and enzymes during fermentation. using gas chromatography time-of-flight mass spectrometry (gc-tof-ms), ultrahigh-performance liquid chromatography linear trap quadrupole ion trap tandem mass spectrometry (uhplc-ltq-it-ms/ms), and multivariate analysis we generated the metabolite profiles of rice koji produced by fermentation with aspergillus oryzae (rk_ao) or bacillus amyloliquefaciens (rk_ ... | 2016 | 27314317 |
| organic acids from root exudates of banana help root colonization of pgpr strain bacillus amyloliquefaciens njn-6. | the successful colonization of plant growth promoting rhizobacteria (pgpr) in the rhizosphere is an initial and compulsory step in the protection of plants from soil-borne pathogens. therefore, it is necessary to evaluate the role of root exudates in the colonization of pgpr. banana root exudates were analyzed by high pressure liquid chromatography (hplc) which revealed exudates contained several organic acids (oas) including oxalic, malic and fumaric acid. the chemotactic response and biofilm f ... | 2015 | 26299781 |
| phylogenetic and comparative sequence analysis of thermostable alpha amylases of kingdom archea, prokaryotes and eukaryotes. | alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. for the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of kingdom archea i.e. pyrococcus furiosus (100-105°c), kingdom prokaryotes i.e. bacillus licheniformis (90-95°c), geobacillus stearothermophilus (75°c), bacillus amyloliquefaciens (72°c), bacill ... | 2014 | 25187685 |
| Impact of rhizosphere factors on cyclic lipopeptide signature from the plant beneficial strain Bacillus amyloliquefaciens S499. | Cyclic lipopeptides of the surfactin, iturin and fengycin families synthesized by plant-associated Bacilli represent an important class of antibiotics since they may be tightly involved in the protective effect of selected strains against phytopathogens. However, their production by Bacillus cells developing on roots under rhizosphere conditions is still poorly understood. In this work, we combined electrospray and imaging mass spectrometry-based approaches to determine the detailed pattern of s ... | 2011 | 22029651 |
| bacterial respiration and growth rates affect the feeding preferences, brood size and lifespan of caenorhabditis elegans. | bacteria serve as live food and nutrients for bacterial-feeding nematodes (bfns) in soils, and influence nematodes behavior and physiology through their metabolism. five bacterial taxa (bacillus amyloliquefaciens jx1, variovorax sp. jx14, bacillus megaterium jx15, pseudomonas fluorescens y1 and escherichia coli op50) and the typical bfn caenorhabditis elegans were selected to study the effects of bacterial respiration and growth rates on the feeding preferences, brood size and lifespan of nemato ... | 2015 | 26222828 |
| augmenting sulfur metabolism and herbivore defense in arabidopsis by bacterial volatile signaling. | sulfur is an element necessary for the life cycle of higher plants. its assimilation and reduction into essential biomolecules are pivotal factors determining a plant's growth and vigor as well as resistance to environmental stress. while certain soil microbes can enhance ion solubility via chelating agents or oxidation, microbial regulation of plant-sulfur assimilation has not been reported. with an increasing understanding that soil microbes can activate growth and stress tolerance in plants v ... | 2016 | 27092166 |
| transcriptomic profiling of bacillus amyloliquefaciens fzb42 in response to maize root exudates. | plant root exudates have been shown to play an important role in mediating interactions between plant growth-promoting rhizobacteria (pgpr) and their host plants. most investigations were performed on gram-negative rhizobacteria, while much less is known about gram-positive rhizobacteria. to elucidate early responses of pgpr to root exudates, we investigated changes in the transcriptome of a gram-positive pgpr to plant root exudates. | 2012 | 22720735 |
| synergistic effect of pseudomonas putida and bacillus amyloliquefaciens ameliorates drought stress in chickpea (cicer arietinum l.). | two plant growth promoting rhizobacteria (pgpr) pseudomonas putida nbrira and bacillus amyloliquefaciens nbrisn13 with ability to tolerate abiotic stress along with multiple pgp traits like acc deaminase activity, minerals solubilisation, hormones production, biofilm formation, siderophore activity were evaluated for their synergistic effect to ameliorate drought stress in chickpea. earlier we have reported both the strains individually for their pgp attributes and stress amelioration in host pl ... | 2015 | 26362119 |
| analysis and cloning of the synthetic pathway of the phytohormone indole-3-acetic acid in the plant-beneficial bacillus amyloliquefaciens sqr9. | the plant growth-promoting rhizobacteria (pgpr) strain bacillus amyloliquefaciens sqr9, isolated from the cucumber rhizosphere, protects the host plant from pathogen invasion and promotes plant growth through efficient root colonization. the phytohormone indole-3-acetic acid (iaa) has been suggested to contribute to the plant-growth-promoting effect of bacillus strains. the possible iaa synthetic pathways in b. amyloliquefaciens sqr9 were investigated in this study, using a combination of chemic ... | 2015 | 26337367 |
| collagen-like proteins (clpa, clpb, clpc, and clpd) are required for biofilm formation and adhesion to plant roots by bacillus amyloliquefaciens fzb42. | the genes of collagen-like proteins (clps) have been identified in a broad range of bacteria, including some human pathogens. they are important for biofilm formation and bacterial adhesion to host cells in some human pathogenic bacteria, including several bacillus spp. strains. interestingly, some bacterial clp-encoding genes (clps) have also been found in non-human pathogenic strains such as b. cereus and b. amyloliquefaciens, which are types of plant-growth promoting rhizobacteria (pgpr). in ... | 2015 | 25658640 |
| enhanced control of cucumber wilt disease by bacillus amyloliquefaciens sqr9 by altering the regulation of its degu phosphorylation. | bacillus amyloliquefaciens strain sqr9, isolated from the cucumber rhizosphere, suppresses the growth of fusarium oxysporum in the cucumber rhizosphere and protects the host plant from pathogen invasion through efficient root colonization. in the gram-positive bacterium bacillus, the response regulator degu regulates genetic competence, swarming motility, biofilm formation, complex colony architecture, and protease production. in this study, we report that stepwise phosphorylation of degu in b. ... | 2014 | 24584252 |
| control efficacy of an endophytic bacillus amyloliquefaciens strain bz6-1 against peanut bacterial wilt, ralstonia solanacearum. | we aimed to isolate and identify endophytic bacteria that might have efficacy against peanut bacterial wilt (bw) caused by ralstonia solanacearum. thirty-seven endophytic strains were isolated from healthy peanut plants in r. solanacearum-infested fields and eight showed antagonistic effects against r. solanacearum. strain bz6-1 with the highest antimicrobial activity was identified as bacillus amyloliquefaciens based on morphology, biochemistry, and 16s rrna analysis. culture conditions of bz6- ... | 2014 | 24527448 |
| biocontrol agent bacillus amyloliquefaciens lj02 induces systemic resistance against cucurbits powdery mildew. | powdery mildew is a fungal disease found in a wide range of plants and can significantly reduce crop yields. bacterial strain lj02 is a biocontrol agent (bca) isolated from a greenhouse in tianjin, china. in combination of morphological, physiological, biochemical and phylogenetic analyses, strain lj02 was classified as a new member of bacillus amyloliquefaciens. greenhouse trials showed that lj02 fermentation broth (lj02fb) can effectively diminish the occurrence of cucurbits powdery mildew. wh ... | 2015 | 26379654 |
| isolation and identification of plant growth promoting rhizobacteria from cucumber rhizosphere and their effect on plant growth promotion and disease suppression. | plant growth promoting rhizobacteria (pgpr) are the rhizosphere bacteria that may be utilized to augment plant growth and suppress plant diseases. the objectives of this study were to identify and characterize pgpr indigenous to cucumber rhizosphere in bangladesh, and to evaluate their ability to suppress phytophthora crown rot in cucumber. a total of 66 isolates were isolated, out of which 10 (ppb1, ppb2, ppb3, ppb4, ppb5, ppb8, ppb9, ppb10, ppb11, and ppb12) were selected based on their in vit ... | 2015 | 26869996 |
| comparative genomic analysis of bacillus amyloliquefaciens and bacillus subtilis reveals evolutional traits for adaptation to plant-associated habitats. | bacillus subtilis and its sister species b. amyloliquefaciens comprise an evolutionary compact but physiologically versatile group of bacteria that includes strains isolated from diverse habitats. many of these strains are used as plant growth-promoting rhizobacteria (pgpr) in agriculture and a plant-specialized subspecies of b. amyloliquefaciens-b. amyloliquefaciens subsp. plantarum, has recently been recognized, here we used 31 whole genomes [including two newly sequenced pgpr strains: b. amyl ... | 2016 | 28066362 |
| recruiting a new strategy to improve levan production in bacillus amyloliquefaciens. | microbial levan is an important biopolymer with considerable potential in food and medical applications. bacillus amyloliquefaciens nk-δlp strain can produce high-purity, low-molecular-weight levan, but production is relatively low. to enhance the production of levan, six extracellular protease genes (bpr, epr, mpr, vpr, npre and apre), together with the tasa gene (encoding the major biofilm matrix protein tasa) and the pgsbca cluster (responsible for poly-γ-glutamic acid (γ-pga) synthesis), wer ... | 2015 | 26347185 |
| detection of the urban release of a bacillus anthracis simulant by air sampling. | in 2005 and 2009, the pentagon force protection agency (pfpa) staged deliberate releases of a commercially available organic pesticide containing bacillus amyloliquefaciens to evaluate pfpa's biothreat response protocols. in concert with, but independent of, these releases, the department of homeland security sponsored experiments to evaluate the efficacy of commonly employed air and surface sampling techniques for detection of an aerosolized biological agent. high-volume air samplers were place ... | 2014 | 24697146 |
| functional characterization and microencapsulation of probiotic bacteria from koozh. | koozh is a traditional fermented millet beverage unique to south india. analysis of six market samples of koozh for their microbial profile resulted in 69 isolates of presumptive lactic acid bacteria (lab). they were grouped as leuconostoc sp., enterococcus sp., streptococcus sp. and lactobacillus sp. based on morphological characteristics and biochemical tests. eight among them showed probiotic features: resistance to acid (2.5 ph for 6 h), resistance to 0.3 % ox bile, moderate hydrophobicity ( ... | 2016 | 27162377 |
| improved production of 2,3-butanediol in bacillus amyloliquefaciens by over-expression of glyceraldehyde-3-phosphate dehydrogenase and 2,3-butanediol dehydrogenase. | previously, a safe strain, bacillus amyloliquefaciens b10-127 was identified as an excellent candidate for industrial-scale microbial fermentation of 2,3-butanediol (2,3-bd). however, b. amyloliquefaciens fermentation yields large quantities of acetoin, lactate and succinate as by-products, and the 2,3-bd yield remains prohibitively low for commercial production. | 2013 | 24098433 |
| study on screening and antagonistic mechanisms of bacillus amyloliquefaciens 54 against bacterial fruit blotch (bfb) caused by acidovorax avenae subsp. citrulli. | bacterial fruit blotch (bfb) was a serious threat to cucurbitaceae crops. it was caused by the gram-negative bacterium acidovorax avenae subsp. citrulli. two hundred strains, which have the potential in controlling plant diseases in our laboratory's biocontrol strain library, were employed to this research to screen some antagonistic bacteria, which can efficiently control bacterial fruit blotch disease. based on the results of antagonistic activity experiments, greenhouse tests and field trials ... | 2015 | 25267487 |
| dietary supplementation with xylanase-expressing b. amyloliquefaciens r8 improves growth performance and enhances immunity against aeromonas hydrophila in nile tilapia (oreochromis niloticus). | bacillus amyloliquefaciens has attracted attention as a probiotic in aquaculture due to its immunostimulatory activity against pathogenic infection. xylanases are extensively used in animal feed to degrade plant ingredients, enhancing nutrient utilization and increasing the growth rate of various animals. in the present study, the effects of dietary supplementation with b. amyloliquefaciens and xylanase-expressing b. amyloliquefaciens r8 on the growth of nile tilapia (oreochromis niloticus) and ... | 2016 | 27670086 |
| diversity and potential application of endophytic bacteria in ginger. | here, 248 endophytic bacterial strains were isolated to assess the distribution and population diversity of endophytic bacteria in ginger plants. a total of 10.4 x 10(4) to 20.2 x 10(4) cfu/g fresh weight endophytic bacteria of different growth stages were isolated. maximum bacterium numbers were obtained at the seedling stage. a total of 107 functional strains were screened, including 17 antibacterial strains and 90 indole acetic acid-producing strains. based on 16s rdna sequence restriction fr ... | 2014 | 25062479 |
| bacillus amyloliquefaciens strain 32a as a source of lipopeptides for biocontrol of agrobacterium tumefaciens strains. | a bacillus amyloliquefaciens strain, designated 32a, was used to identify new compounds active against agrobacterium tumefaciens and to evaluate their efficiency to control crown gall on carrot discs. | 2015 | 25764969 |
| imaging mass spectrometry of a coral microbe interaction with fungi. | fungal infections are increasing worldwide, including in the aquatic environment. microbiota that coexist with marine life can provide protection against fungal infections by secretion of metabolites with antifungal properties. our laboratory has developed mass spectrometric methodologies with the goal of improving our functional understanding of microbial metabolites and guiding the discovery process of anti-infective agents from natural sources. ga40, a bacillus amyloliquefaciens strain isolat ... | 2013 | 23881443 |
| antifungal activity of isolated bacillus amyloliquefaciens sybc h47 for the biocontrol of peach gummosis. | the gummosis disease is caused by botryosphaeria dothidea (moug. ex. fr) ces. et de not., and it is one of the most important diseases of stone fruits worldwide. the use of biocontrol as an alternative approach to synthetic chemical fungicides has aroused general concern about how to control plant diseases that are caused by phytopathogens. the aim of this study is to isolate bacillus strains from raw honeys with the capacity to inhibit b. dothidea and to explore the mechanisms by which they cou ... | 2016 | 27583463 |
| cultivable microorganisms associated with honeys of different geographical and botanical origin. | in this study, the composition of the cultivable microbial populations of 38 nectar honey and honeydew honey samples of different botanical and geographical origin were assessed. after growth in specific media, various colonies with different appearance were isolated and purified before phenotypic (morphological, physiological and biochemical traits) and genotypic [randomly amplified polymorphic dna (rapd), repetitive dna elements-pcr (rep-pcr) and restriction fragment length polymorphism (rflp) ... | 2014 | 24290653 |
| effect of neohesperidin dihydrochalcone on the activity and stability of alpha-amylase: a comparative study on bacterial, fungal, and mammalian enzymes. | neohesperidin dihydrochalcone (nhdc) was recently introduced as an activator of mammalian alpha-amylase. in the current study, the effect of nhdc has been investigated on bacterial and fungal alpha-amylases. enzyme assays and kinetic analysis demonstrated the capability of nhdc to significantly activate both tested alpha-amylases. the ligand activation pattern was found to be more similar between the fungal and mammalian enzyme in comparison with the bacterial one. further, thermostability exper ... | 2015 | 25808616 |
| trypsin inhibitor from edible mushroom pleurotus floridanus active against proteases of microbial origin. | protease inhibitors can be versatile tools mainly in the fields of medicine, agriculture and food preservative applications. fungi have been recognized as sources of protease inhibitors, although there are only few such reports on mushrooms. this work reports the purification and characterization of a trypsin inhibitor from the fruiting body of edible mushroom pleurotus floridanus (pfti) and its effect on the activity of microbial proteases. the protease inhibitor was purified up to 35-fold by d ... | 2014 | 24615526 |
| analysis of microflora profile in korean traditional nuruk. | a variety of nuruk were collected from various provinces in korea, and their microflora profiles were analyzed at the species level. a total of 42 nuruk samples were collected and when the viable cell numbers in these nuruk were enumerated, the average cell numbers of bacteria, fungi, yeast, and lactic acid bacteria from all nuruk were 7.21, 7.91, 3.49, and 4.88 log cfu/10 g, respectively. there were no significant differences in viable cell numbers of bacteria or fungi according to regions coll ... | 2013 | 23314366 |
| production of itaconic acid in escherichia coli expressing recombinant α-amylase using starch as substrate. | several studies on fermentative production of a vinyl monomer itaconic acid from hydrolyzed starch using aspergillus terreus have been reported. herein, we report itaconic acid production by escherichia coli expressing recombinant α-amylase, using soluble starch as its sole carbon source. to express α-amylase in e. coli, we first constructed recombinant plasmids expressing α-amylases by using cell surface display technology derived from two amylolytic bacteria, bacillus amyloliquefaciens nbrc 15 ... | 2015 | 25468427 |
| biodiversity of aerobic endospore-forming bacterial species occurring in yanyanku and ikpiru, fermented seeds of hibiscus sabdariffa used to produce food condiments in benin. | yanyanku and ikpiru made by the fermentation of malcavene bean (hibiscus sabdariffa) are used as functional additives for parkia biglobosa seed fermentations in benin. a total of 355 aerobic endospore-forming bacteria (aefb) isolated from yanyanku and ikpiru produced in northern and southern benin were identified using phenotypic and genotypic methods, including gtg5-pcr, m13-pcr, 16s rrna, gyra and gyrb gene sequencing. generally, the same 5-6 species of the genus bacillus predominated: bacillu ... | 2013 | 23571124 |
| production of bioactive hydrolysate using protease, β-glucosidase and α-amylase of bacillus spp. isolated from kinema. | the aim of this study was the production of soybean bioactive hydrolysate using bacillus spp. isolated from kinema. totally 251 bacteria isolated from kinema samples, collected at different time period were screened for protease, β-glucosidase and α-amylase activities and further identified by ardra based grouping followed by analysis of 16s rrna gene sequence similarity. the results showed that bacillus subtilis, bacillus amyloliquefaciens and bacillus licheniformis were the major bacillus spec ... | 2017 | 28384588 |
| probiotic bacillus amyloliquefaciens sc06 induces autophagy to protect against pathogens in macrophages. | probiotics are increasingly applied in popularity in both humans and animals. decades of research has revealed their beneficial effects, including the immune modulation in intestinal pathogens inhibition. autophagy-a cellular process that involves the delivery of cytoplasmic proteins and organelles to the lysosome for degradation and recirculation-is essential to protect cells against bacterial pathogens. however, the mechanism of probiotics-mediated autophagy and its role in the elimination of ... | 2017 | 28382029 |
| intramembrane protease rasp boosts protein production in bacillus. | the microbial cell factory bacillus subtilis is a popular industrial platform for high-level production of secreted technical enzymes. nonetheless, the effective secretion of particular heterologous enzymes remains challenging. over the past decades various studies have tackled this problem, and major improvements were achieved by optimizing signal peptides or removing proteases involved in product degradation. on the other hand, serious bottlenecks in the protein export process per se remained ... | 2017 | 28376795 |
| production of surfactin from waste distillers' grains by co-culture fermentation of two bacillus amyloliquefaciens strains. | distillers' grains (dgs), the main waste by-products of chinese liquor industry, were used as substrate for surfactin production. bacillus amyloliquefaciens mt45 could grow with dgs as sole carbon source to produce 1.04g/l surfactin. however, low amylase activity of mt45 limited sugar supply and the subsequent surfactin production. therefore, mt45 was co-cultured with bacillus strains that exhibited remarkable hydrolases activities. surfactin yield increased by 50% when mt45 was co-cultured with ... | 2017 | 28365354 |
| production of exopolysaccharides by selected bacillus strains: optimization of media composition to maximize the yield and structural characterization. | selected bacillus strains were investigated for the production of exopolysaccharides (eps) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (m1), (ii) succinate-containing mineral base-medium with added yeast extract (m2) and (iii) tryptone and yeast extract-containing base-medium (m3). modest eps yield of 6.7g/l was recorded for bacillus amyloliquefaciens 23350 grown on m1, where the eps produced was characterized by a low mw (<5kda) and of bein ... | 2017 | 28363654 |
| plant growth-promoting endophytic bacteria versus pathogenic infections: an example of bacillus amyloliquefaciens rwl-1 and fusarium oxysporum f. sp. lycopersici in tomato. | fungal pathogenic attacks are one of the major threats to the growth and productivity of crop plants. currently, instead of synthetic fungicides, the use of plant growth-promoting bacterial endophytes has been considered intriguingly eco-friendly in nature. here, we aimed to investigate the in vitro and in vivo antagonistic approach by using seed-borne endophytic bacillus amyloliquefaciens rwl-1 against pathogenic fusarium oxysporum f. sp. lycopersici. the results revealed significant suppressio ... | 2017 | 28321368 |