| organization of the antiseptic resistance gene qaca and tn552-related beta-lactamase genes in multidrug- resistant staphylococcus haemolyticus strains of animal and human origins. | a part (12 kb) of a plasmid containing the beta-lactamase genes of tn552, the disinfectant resistance gene qaca, and flanking dna has been cloned from a staphylococcus haemolyticus isolate and sequenced. this region was used to map the corresponding regions in six other multiresistant s. haemolyticus isolates of human and animal origin. the organizations of the genetic structures were almost identical in all isolates studied. the beta-lactamase and qaca genes from s. haemolyticus have >99.9% ide ... | 2002 | 12384372 |
| sensitivity and rapidity of blood culture bottles in the detection of cornea organ culture media contamination by bacteria and fungi. | to test the bactericidal activity of standard organ culture medium, and to compare the sensitivity and rapidity of blood culture bottles with conventional microbiological methods for detection of bacteria and fungi inoculated in a standard cornea organ culture medium. | 2002 | 12446379 |
| in vitro activity of quinupristin/dalfopristin, linezolid, telithromycin and comparator antimicrobial agents against 13 species of coagulase-negative staphylococci. | to determine in vitro susceptibilities of a large series of speciated coagulase-negative staphylococci (cns) against three new antibiotics, linezolid, quinupristin/dalfopristin and telithromycin. | 2002 | 12461014 |
| [staphylococcus haemolyticus and bacteremia]. | | 2002 | 12582440 |
| survey of methicillin-resistant coagulase-negative staphylococci isolated from the fingers of nursing students. | to clarify the state of methicillin-resistant coagulase-negative staphylococci (mrcns) contamination in the hospital environment, we compared mrcns isolated from the fingers of 40 nursing students who had not yet experienced clinical practice and 40 who had just completed clinical practice in the hospital. fourteen mrcns strains were detected in 13 students (32.5%) after clinical practice; staphylococcus epidermidis in 9 students, staphylococcus haemolyticus in 3, and staphylococcus saprophyticu ... | 2003 | 12673404 |
| use of drotrecorgin alfa--recombinant activated human protein c in treatment of septic shock in the course of therapy for nephrolithiasis. | septic shock is the most dangerous complication of nephrolithiasis management utilizing percutaneous methods. | 2003 | 12761458 |
| [species affiliation of staphylococci isolated in purulent infections]. | the microflora was studied of the clinical material taken from those patients presenting with different pyo-septic diseases. it has been ascertained that a key role in the etiology of the above diseases must be referred to gramme-positive micro-organisms--to coagulase-negative staphylococci among their number. of these, s. haemolyticus and s. epidermidis were found with high frequency. | 2003 | 12889369 |
| application of molecular typing methods to characterize nosocomial coagulase-negative staphylococci collected in a greek hospital during a three-year period (1998-2000). | a total of 143 methicillin-resistant coagulase-negative staphylococci (mr-cns) collected between 1998 and 2000 at the university hospital of patras, greece, were characterized by antibiogram and genomic typing to define the clonal types endemic in this hospital and their evolution during the 3-year period. these isolates corresponded to 93 methicillin-resistant staphylococcus epidermidis (mrse) and 50 other mr-cns, which were isolated from patients in different wards, exclusively from blood and ... | 2003 | 12959406 |
| [prostate histopathology of nih category iv prostatitis detected by sextant prostate needle biopsy from the patients with high prostatic specific antigen]. | asymptomatic prostatitis is classified as category iv in nih classification of prostatitis syndrome (1999). no report concerning this category has been present. we investigated this category histopathologically and clinically, in order to clarify the histopathological distribution and its correlation to the clinical features, in this study. among 785 patients who were suspected prostate cancer because of their high prostatic specific antigen (psa) values and to have a sextant prostate needle bio ... | 2003 | 14515754 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2001--i. gram-positive bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2001 were yearly evaluated and compared with those of other cephems, oxacephems, carbapenems, and penicillins. a total of 1,274 strains in 15 species of gram-positive bacteria were isolated from the clinical materials annually collected from january to december, and consisted of methicillin-susceptible staphylococcus aureus (mssa), methicillin-resistant sta ... | 2003 | 14567254 |
| intestinal microflora in early infancy: composition and development. | the neonatal intestinal microbiota is a complex ecosystem composed of numerous genera, species and strains of bacteria. this enormous cell mass performs a variety of unique activities that affect both the colonic and systemic physiology. its primary activities include nutritive, metabolic, immunological and protective functions. most studies of infants have been based on faecal samples using the classical plating techniques with culturing on specific media. the limitations of these methods must ... | 2003 | 14599042 |
| isolation and molecular characterization of multiresistant staphylococcus sciuri and staphylococcus haemolyticus associated with skin and soft-tissue infections. | the isolation, molecular identification and genotyping of multiresistant staphylococcus sciuri and staphylococcus haemolyticus from skin and soft-tissue infections are reported. accurate and full identification of three coagulase-negative staphylococcal isolates was achieved using pcr, while the api staph method failed to identify an isolate of s. haemolyticus fully. the pcr assay, which detects polymorphism in the 16s-23s rrna spacer region, is shown to be potentially useful for rapid and accur ... | 2004 | 14663105 |
| [post-marketing surveillance of antibacterial activities of cefozopran against various clinical isolates--i. gram-positive bacteria]. | as a post-marketing surveillance, the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates were yearly evaluated and compared with those of other cephems, oxacephems, penicillins, and carbapenems. changes in the bacterial susceptibility for czop were also evaluated with the resistance ratio calculated with breakpoint mic. sixteen species (2,363 strains) of gram-positive bacteria were isolated from the clinical materials annually collected ... | 2003 | 14692380 |
| [selected features determine pathogenicity of staphylococcus haemolyticus]. | the study have been done on s. haemolyticus strains isolated from patients hospitalized an surgical unit. aim of the study was to determine pathogenic traits of s. haemolyticus: slime producing, adhesion to biomaterials, antibiotics susceptibility and the profiles of surface proteins. among 44 s. haemolyticus strains, in the test-tube method, there have been 38% labeled as slime producing and 62% as non-producing. in the plate method at 48% slime production was noticed, while 52% strains did not ... | 2003 | 14702664 |
| [composition of ame encoding gene in gram positive cocci--study on spread of aminoglycoside resistance]. | aminoglycoside modifying enzymes (ames) are major factors which confer aminoglycoside resistance on bacteria. composition of six genes encoding ames (including lately described aph 2"-genes) was investigated by pcr for 16 clinical isolates of enterococcus faecalis, 16 clinical isolates of coagulase-positive (s. aureus) and 13 clinical isolates of coagulase negative staphylococci (s. haemolyticus, s. epidermidis) collected in gdańsk region (northern poland) in the years 1998-2001. diversity of am ... | 2003 | 15103988 |
| [prevalence of aa(6')-aph(2")ia gene among high-level aminoglycoside resistant enterococci and staphylococci]. | according to new reports the aac (6')-aph (2")ia gene is no longer the only gene encoding resistance to gentamycin in gram-positive cocci and therefore the current method for predicting synergism aminoglycosides with bacterial cell wall active agents in this bacteria may need revision. to further our knowledge of aminoglycoside resistance mechanism in gram-positive cocci in gdańsk region we tested presence of aac (6')-aph (2")ia gene among 22 enterococcal (e. faecalis) and 41 staphylococcal (s. ... | 2003 | 15103989 |
| defective neutrophil oxidative burst in preterm newborns on exposure to coagulase-negative staphylococci. | the neutrophil oxidative burst is a product of the regulated assembly of the multicomponent oxidase enzyme. our aim was to compare the oxidative burst in term (n = 10) and preterm newborns <31 wk gestational age (n = 10) after stimulation with coagulase-negative staphylococci in vitro. strains of streptococcus epidermidis with different invasive and slime-producing properties, one strain of s. haemolyticus, and one strain of group b-streptococcus were investigated. a whole-blood flow cytometric ... | 2004 | 15155865 |
| glycopeptide resistance in staphylococcus aureus: is it a real threat? | following the discovery in 1992 that resistance to vancomycin could be transferred in vitro from a strain of staphylococcus haemolyticus to staphylococcus aureus, the threat that a similar event could happen in vivo has existed. in 1996, the threat became a reality in japan followed by reports of low-level heteroresistant s. aureus from elsewhere in the world. however, the threat has not gone away; indeed it has become more intimidating within the past 2 years with the isolation of higher-level ... | 2004 | 15160298 |
| in vitro combined bactericidal activity of cefpirome and glycopeptides against glycopeptides and oxacillin-resistant staphylococci. | infections caused by coagulase-negative staphylococci are becoming increasingly important, particularly those of nosocomial origin, as the organisms are frequently multi-resistant. new antimicrobial strategies are needed. the bactericidal activity of a combination of cefpirome with either vancomycin or teicoplanin against 12 strains of methicillin-resistant staphylococci with a decreased susceptibility to teicoplanin was determined in vitro by a time killing method. strains mu3 and mu50 of staph ... | 2004 | 15164965 |
| purification and characterization of recombinant staphylococcus haemolyticus dna gyrase and topoisomerase iv expressed in escherichia coli. | the subunits of dna gyrase and topoisomerase iv from staphylococcus haemolyticus were expressed in escherichia coli, purified to homogeneity, and used to reconstitute active enzymes that were sensitive to known topoisomerase inhibitors. this represents the first description of a method for isolating type ii topoisomerases of a coagulase-negative staphylococcal species. | 2004 | 15215131 |
| evaluation of the uro-quick, a new rapid automated system, for the detection of well-characterized antibiotic-resistant bacteria. | the uro-quick system has been employed to detect antibiotic resistance in genotypically and/or phenotypically well-characterized bacterial species including those that might not be easily identified by routine procedure. in order to achieve full agreement between the antibiotic susceptibility results obtained by the reference method (nccls) and the uro-quick system, the optimal experimental conditions (inoculum size, time of incubation and antibiotic concentration) for each strain to be used by ... | 2004 | 15216942 |
| antimicrobial-resistance and enterotoxin-encoding genes among staphylococci isolated from expressed human breast milk. | resistance traits and the presence of enterotoxin-encoding genes were investigated in staphylococcus isolates obtained from expressed human breast milk. a total of 54 staphylococcal isolates identified as staphylococcus epidermidis (53.6 %), staphylococcus warneri (20.4 %), staphylococcus haemolyticus (13 %) and staphylococcus aureus (13 %) were investigated. by using a disc-diffusion method, higher rates of resistance, including intermediate resistance, were observed for penicillin (87 %) and e ... | 2004 | 15272063 |
| comparison of the bbl chromagar staph aureus agar medium to conventional media for detection of staphylococcus aureus in respiratory samples. | screening for staphylococcus aureus has become routine in certain patient populations. this study is the first clinical evaluation of the bbl chromagar staph aureus agar (csa) medium (bd diagnostics, sparks, md.) for detection of s. aureus in nasal surveillance cultures and in respiratory samples from cystic fibrosis (cf) patients. s. aureus colonies appear mauve on csa. other organisms are inhibited or produce a distinctly different colony color. s. aureus was identified from all media by slide ... | 2004 | 15297498 |
| differential regulation of th1/th2 in relevant tissues for sepsis pathogenesis with a limulus anti-lps factor-derived peptide increases survival in gram-positive sepsis. | severe sepsis and septic shock are important causes of death in intensive care units. although gram-negative infections were predominant in the 1960s, gram-positive infections have increased in the past two decades and now account for about half of the cases of severe sepsis. in this study, we examined the effect of a limulus anti-lps factor (lalf)-derived peptide on lung and liver th1/th2 cytokine mrna levels during a gram-positive sepsis. we also examined the morphopathological changes observe ... | 2004 | 15313432 |
| prevalence of methicillin-resistant, coagulase-negative staphylococci in neonatal intensive care units: findings from a tertiary care hospital in india. | this study was undertaken to determine the antimicrobial resistance pattern and species of coagulase-negative staphylococci (cns) isolated from the blood and skin of neonates with clinical suspicion of late-onset septicaemia (>72 h post-delivery) admitted to neonatal intensive care units, with particular reference to the phenotypic and genotypic expression of methicillin resistance. blood culture specimens were collected by venipuncture from 660 such neonates in brain heart infusion broth. skin ... | 2004 | 15314204 |
| methicillin-resistant staphylococci isolated from animals. | staphylococci isolated from animals (n=311) were screened for methicillin resistance by oxacillin agar screening. oxacillin-resistant strains were tested for the presence of the meca gene by pcr. isolates were identified by standard techniques and 16s rdna analysis, and their antimicrobial susceptibilities were tested using an agar diffusion method. meca-positive strains were further analyzed using pulsed-field gel electrophoresis (pfge). from 11 multidrug-resistant staphylococci, 6 were meca-po ... | 2004 | 15381271 |
| comparative evaluation of coagulase-negative staphylococci (cons) adherence to acrylic by a static method and a parallel-plate flow dynamic method. | the main goal of this work was to evaluate the adhesion to acrylic of several clinical strains of staphylococcus epidermidis and staphylococcus haemolyticus using both static and dynamic adhesion methods, and to compare the results obtained with these two methods. adhesion was evaluated using the static slide method with different washing procedures, and the parallel plate flow chamber method. the extent of s. epidermidis adhesion, assessed by both methods, was greater than that of s. haemolytic ... | 2004 | 15501653 |
| antimicrobial efficacy of a new antibiotic-loaded poly(hydroxybutyric-co-hydroxyvaleric acid) controlled release system. | failure of orthopaedic devices, mainly femoral hip replacements, due to infection is of increasing medical importance. there is a need for improved antibiotic delivery systems in the treatment of orthopaedic infections and here we have evaluated polyhydroxyalkanoate formulations for their suitability as a constant delivery system for gentamicin. | 2004 | 15537698 |
| genomic characterization of oxacillin-resistant staphylococcus epidermidis and staphylococcus haemolyticus isolated from brazilian medical centres. | studies on the genetic diversity of oxacillin-resistant coagulase-negative staphylococcal (cns) isolates are important for the control and prevention of infections. the present study evaluated the clonal diversity of oxacillin-resistant staphylococcus epidermidis (orse) and staphylococcus haemolyticus (orsh) strains, isolated from patients in nine brazilian medical centres by using pulsed-field gel electrophoresis (pfge) after digestion of bacterial dna using smai. pfge analysis of orse (n=44) a ... | 2005 | 15571849 |
| evaluation of phenotypic methods for methicillin resistance characterization in coagulase-negative staphylococci (cns). | coagulase-negative staphylococci (cns) are the major cause of nosocomial infections. methicillin-resistant strains are particularly important because they narrow therapeutic options. detecting methicillin resistance among cns has been a challenge for years. the objective of this study was to determine the accuracy of an agar screening test (0.6 and 4 microg oxacillin ml(-1)), disc diffusion and the automated microscan system to characterize methicillin resistance among cns. one hundred and seven ... | 2004 | 15585497 |
| the binding of mbl to common bacteria in infectious diseases of children. | to purify mannan-binding lectin (mbl) from human serum and detect its binding ability to several kinds of bacteria common in infectious diseases of children. | 2005 | 15593393 |
| early bacterial keratitis after laser-assisted subepithelial keratectomy. | we report a case of bacterial keratitis that occurred after laser-assisted subepithelial keratectomy. the patient presented with a decrease in visual acuity and pain 2 days after the procedure. culture was positive for staphylococcus haemolyticus. the infiltrate slowly resolved with topical antibiotics, and the best corrected visual acuity improved to 20/20. although bacterial keratitis occurs rarely after refractive surgery, patients should be informed of the potential risk for visual loss caus ... | 2004 | 15617940 |
| rapid detection of methicillin resistance in teicoplanin-resistant coagulase-negative staphylococci by a penicillin-binding protein 2' latex agglutination method. | thirty-five clinical isolates of coagulase-negative staphylococci with decreased glycopeptide sensitivity were examined by a penicillin-binding protein (pbp2') latex agglutination (la) test and were compared to the detection of the meca gene by pcr, and oxacillin susceptibility determined minimum inhibitory concentrations. the latex test demonstrated high sensitivity and specificity for detecting methicillin resistance in coagulase-negative staphylococci after pbp2' induction with oxacillin. | 2005 | 15649543 |
| evaluation of six commercial identification kits for the identification of staphylococcus aureus isolated from bovine mastitis. | comparison of six commercially available in human medicine well-established slide agglutination systems for the identification of staphylococcus aureus. | 2005 | 15659199 |
| spread of clones of multidrug-resistant, coagulase-negative staphylococci within a university hospital. | to detect putative clonal dissemination of multidrug-resistant, coagulase-negative staphylococci (cns) in a university hospital in northern sweden. | 2005 | 15693412 |
| the discovery of a multi-resistant staphylococcus haemolyticus clone in the hospital and community environment in south western nigeria. | among clinically significant isolates of coagulase negative staphylococci, staphylococcus haemolyticus is ranked second after staphylococcus epidermidis. it has been associated with septicemia in newborns and various infections in persons with compromised host defenses and implanted foreign bodies. the existence of a multi-resistant staphylococcus haemolyticus clone was discovered during a study on patients with skin and soft tissue infections at two local health clinics and in a referral hospit ... | 2005 | 15695837 |
| methicillin-resistant staphylococcal bacteremia in patients with hematologic malignancies: clinical and microbiological retrospective comparative analysis of s. haemolyticus, s. epidermidis and s. aureus. | although staphylococcus haemolyticus (sh) represents an emerging etiology of methicillin-resistant (mr) coagulase-negative staphylococcal nosocomial bacteremia, little is known of clinical significance of this infection. thus, we performed case-control retrospective comparative analysis of mrsh bacteremias (mrshb), methicillin-resistant s. epidermidis bacteremias (mrseb), and methicillin-resistant s. aureus bacteremias (mrsab) in patients with hematologic malignancies. most patients in the three ... | 2004 | 15700845 |
| identification of coagulase-negative staphylococci other than staphylococcus epidermidis by automated ribotyping. | as routine identification of coagulase-negative staphylococci is problematic, the performance of automated ribotyping was evaluated for identification of coagulase-negative staphylococci other than staphylococcus epidermidis. in total, 177 isolates were tested, comprising 149 isolates from blood samples, 15 isolates that were not identified by internal transcribed spacer (its)-pcr in a previous study, and 13 reference strains. the identification results were compared with those obtained by the a ... | 2005 | 15715714 |
| erroneous reporting of coagulase-negative staphylococci as kocuria spp. by the vitek 2 system. | misidentification of coagulase-negative staphylococci (cons) may delay appropriate treatment. we investigated 20 clinical isolates identified as kocuria spp. by the vitek 2 system. all were identified as cons by 16s rrna gene sequencing (18 staphylococcus epidermidis, 1 staphylococcus haemolyticus). four kocuria isolates were shown to be identical to cons from the same patient by pulsed-field gel electrophoresis. isolates identified by vitek 2 as kocuria most likely represent misidentified cons, ... | 2005 | 15750130 |
| [adhesion dependence of coagulase-negative staphylococci to biomaterials and pia polysaccharide synthesis on icaadbc operon presence]. | both staphylococcus epidermidis and staphylococcus haemolyticus are important causes of infections associated with catheters and other medical devices. this infections result in significant morbidity, mortality and economic cost. it has recently been shown that not only s. epidermidis but also s. haemolyticus can produce slime and carries the ica operon responsible for and slime production. in the operon, coexpression of icaa and icad is required for full slime synthesis. this study is focused o ... | 2004 | 15773498 |
| bilateral adrenocortical uptake of ga-67 spect during septicemia in a heart transplant patient. | gallium-67 scintigraphy is a valuable agent in the management of fever of unknown origin. the use of spect increases its sensitivity and may demonstrate unexpected findings. we report on a heart-transplanted 55-year-old man with postsurgical fever of unknown origin. ga-67 spect showed bilateral abnormal adrenal gland uptake that disappeared after intensive antibiotic therapy as assessed by a new ga-67 scintigraphy obtained 3 months later. unilateral and bilateral adrenal uptake of gallium has be ... | 2005 | 15827412 |
| coagulase-negative staphylococcal meningitis in adults: clinical characteristics and therapeutic outcomes. | we wanted to analyze the clinical characteristics and therapeutic outcomes of adult meningitis caused by coagulase-negative staphylococci (cons). | 2005 | 15827871 |
| investigation of staphylococcus strains with heterogeneous resistance to glycopeptides in a turkish university hospital. | the hetero-glycopeptide intermediate staphylococci is considered to be the precursor of glycopeptide intermediate staphylococci especially vancomycin intermediate staphylococcus aureus (visa). for this purpose, we aimed to investigate the heterogeneous resistance to glycopeptide and their frequencies in 135 staphylococcus strains. | 2005 | 15871748 |
| microarray-based detection of 90 antibiotic resistance genes of gram-positive bacteria. | a disposable microarray was developed for detection of up to 90 antibiotic resistance genes in gram-positive bacteria by hybridization. each antibiotic resistance gene is represented by two specific oligonucleotides chosen from consensus sequences of gene families, except for nine genes for which only one specific oligonucleotide could be developed. a total of 137 oligonucleotides (26 to 33 nucleotides in length with similar physicochemical parameters) were spotted onto the microarray. the micro ... | 2005 | 15872258 |
| components and antibacterial activity of the roots of salvia jaminiana. | the acetone extract of the roots of salvia jaminiana, containing the sterols campestanol, stigmasterol and sitosterol, and five known diterpenoids, ferruginol, cryptanol, 6,7-dehydroroyleanon, 6-hydroxysalvinolone and microstegiol, remarkably inhibited the growth of bacillus subtilis, staphylococcus aureus atcc 25923 and streptococcus alpha-hemolitic. | 2005 | 15893885 |
| comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid xtt colorimetry. | to quantitatively compare the antibiotic susceptibility of biofilms formed by the coagulase-negative staphylococci (cons) staphylococcus epidermidis and staphylococcus haemolyticus with the susceptibility of planktonic cultures. | 2005 | 15980094 |
| genotypic versus phenotypic characterization, with respect to susceptibility and identification, of 17 clinical isolates of staphylococcus lugdunensis. | to compare different methods for the identification and determination of susceptibility to penicillin and methicillin of staphylococcus lugdunensis. | 2005 | 15994248 |
| a study of the pattern of staphylococcal contamination of the udders of cows in a dairy shed. | | 1972 | 16030620 |
| antimicrobial susceptibility, biochemical and genetic profiles of staphylococcus haemolyticus strains isolated from the bloodstream of patients hospitalized in critical care units. | staphylococcus haemolyticus strains (n=20), responsible of blood stream infections, were consecutively isolated from patients hospitalized in two different wards at high risk of infection. strains displayed high rate of resistance to oxacillin (90%). all strains but two with decreased susceptibility (mic = 4 microg/ml), were sensitive to vancomycin. ten strains were resistant to teicoplanin. among the strains susceptible to glycopeptides, three displayed heteroresistance to vancomycin and seven ... | 2005 | 16038519 |
| prevalence of resistance mechanisms against macrolides and lincosamides in methicillin-resistant coagulase-negative staphylococci in the czech republic and occurrence of an undefined mechanism of resistance to lincosamides. | high occurrence of the non-macrolide-lincosamide-streptogramin b resistance genes msra (53%) and lina/lina' (30%) was found among 98 methicillin-resistant coagulase-negative staphylococci additionally resistant to macrolides and/or lincosamides. the gene msra predominated in staphylococcus haemolyticus (43 of 62 isolates). in staphylococcus epidermidis, it was present in 7 of 27 isolates. a novel mechanism of resistance to lincosamides appears to be present in 10 genetically related isolates of ... | 2005 | 16048992 |
| pd-140248 (parke-davis & co). | pd-140248 is an isomerase inhibitor and topoisomerase ii inhibitor under development by parke-davis and co as a potential treatment for bacterial infection. although no details of active development have been published in the scientific literature since 1996, in september 1999, the company confirmed that pd-140248 is still in active development [338530]. the compound is one of two new pyrrolidinyl naphthyridines (the other compound being pd-131628) with a broad spectrum of antibacterial activity ... | 2000 | 16107942 |
| increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in the gaza strip. | this study aims to assess common organisms causing urinary tract infection (uti) in the gaza strip, palestinian authority and to examine the incidence of ciprofloxacin resistance in the strains of bacteria isolated from patients suspected with uti over a six-month period. | 2005 | 16123829 |
| survey of extreme solvent tolerance in gram-positive cocci: membrane fatty acid changes in staphylococcus haemolyticus grown in toluene. | we exploited the unique ecological niche of oil fly larval guts to isolate a strain of staphylococcus haemolyticus which may be the most solvent-tolerant gram-positive bacterium yet described. this organism is able to tolerate 100% toluene, benzene, and p-xylene on plate overlays and saturating levels of these solvents in monophasic liquid cultures. a comparison of membrane fatty acids by gas chromatography after growth in liquid media with and without toluene showed that in cells continuously e ... | 2005 | 16151101 |
| increase of faecal bifidobacteria due to dietary oligosaccharides induces a reduction of clinically relevant pathogen germs in the faeces of formula-fed preterm infants. | in a previous study on formula-fed preterm infants, we were able to demonstrate that dietary oligosaccharides (a mixture of 90% galacto-oligosaccharides and 10% fructo-oligosaccharides in a concentration of 1 g/dl) stimulate the growth of faecal bifidobacteria. in the present explorative analysis of this study, we focus on the effect of the dominance of bifidobacteria on the presence of clinically relevant pathogens (staphylococcus aureus, staphylococcus epidermidis, staphylococcus haemolyticus, ... | 2005 | 16214763 |
| spectrum and antibiotic resistance of uropathogens isolated from hospital and community patients with urinary tract infections in two large hospitals in kuwait. | to determine the spectrum of microbial etiology and antibiotic resistance pattern of the uropathogens that cause urinary tract infections in 2 large teaching hospitals in kuwait over a period of 1 year. | 2005 | 16220013 |
| whole-genome sequencing of staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species. | staphylococcus haemolyticus is an opportunistic bacterial pathogen that colonizes human skin and is remarkable for its highly antibiotic-resistant phenotype. we determined the complete genome sequence of s.haemolyticus to better understand its pathogenicity and evolutionary relatedness to the other staphylococcal species. a large proportion of the open reading frames in the genomes of s.haemolyticus, staphylococcus aureus, and staphylococcus epidermidis were conserved in their sequence and order ... | 2005 | 16237012 |
| evaluation of spontaneous resistance to glycopeptide antibiotics in staphylococcal populations. | | 1989 | 16312453 |
| effects of growth in the presence of subinhibitory concentrations of dicloxacillin on staphylococcus epidermidis and staphylococcus haemolyticus biofilms. | low concentrations of antibiotics can inhibit microbial adherence to medical device surfaces. however, little is known about the changes that occur in the physiology of bacteria within biofilms formed in the presence of subinhibitory (sub-mic) concentrations of antibiotics. in this study, the densities and matrix compositions of biofilms formed by two coagulase-negative staphylococcus species in the absence and in the presence of sub-mic concentrations of dicloxacillin were evaluated. biofilms f ... | 2005 | 16332862 |
| teicoplanin use and emergence of staphylococcus haemolyticus: is there a link? | | 2006 | 16460556 |
| identification and molecular characterization of mannitol salt positive, coagulase-negative staphylococci from nasal samples of medical personnel and students. | the identification of mannitol salt positive, coagulase-negative staphylococci (cns) is often disregarded when staphylococcus aureus is screened in clinical samples using mannitol salt agar. however, the emergence of cns as important human pathogens has indicated that reliable methods for the identification of clinically significant cns are of great importance in understanding the epidemiology of infections caused by them. the identification and molecular characterization of mannitol salt positi ... | 2006 | 16476796 |
| study of the efficacy of coated vicryl plus antibacterial suture (coated polyglactin 910 suture with triclosan) in two animal models of general surgery. | to evaluate the efficacy in vitro and in vivo of a new antibacterial suture (pgab) compared with a traditional braided suture (pg). our primary goals were to study microbiological effectiveness and impact on wound healing of pgab vs pg. secondary goal was to analyze influence on inflammatory response. | 2007 | 16487594 |
| heterogeneous resistance to vancomycin in staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening. | the population analysis profile (pap) method as well as analysis of autolytic activity and cellular ultrastructure by transmission electron microscopy (tem) were used to characterise staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus warneri clinical strains with reduced susceptibility to glycopeptides. all strains showed heterogeneous profiles to vancomycin and teicoplanin by the pap method. subpopulations that grew in the presence of high concentrations of each drug wer ... | 2006 | 16542825 |
| tabebuia avellanedae naphthoquinones: activity against methicillin-resistant staphylococcal strains, cytotoxic activity and in vivo dermal irritability analysis. | methicillin-resistant staphylococcus aureus (mrsa) and coagulase-negative staphylococcus infections are a worldwide concern. currently, these isolates have also shown resistance to vancomycin, the last therapy used in these cases. it has been observed that quinones and other related compounds exhibit antibacterial activity. this study evaluated the antibacterial activity, toxicity and in vivo dermal irritability of lapachol extracted from tabebuia avellanedae and derivatives against methicillin- ... | 2006 | 16553949 |
| coagulase-negative staphylococci in very-low-birth-weight infants: inability of genetic markers to distinguish invasive strains from blood culture contaminants. | selected coagulase-negative staphylococci from the blood of very-low-birth-weight infants in the neonatal intensive care unit at the royal women's hospital, melbourne, collected over a 5-year period were examined. isolates were classified as invasive or contaminants, speciated, typed by pulsed-field gel electrophoresis, and examined for biofilm genes (icaa, icac, and icad), adhesion genes (atle, fbe), and the number of copies of is256. of the 24 isolates studied, there were 13 contaminants and 1 ... | 2006 | 16598472 |
| invasive potential of bacterial isolates associated with subclinical bovine mastitis. | this work describes differences in the invasive ability of bacterial isolates associated with mastitis. invasion ability was determined by the uptake and survival in a primary culture of bovine mammary epithelial cells (bmec). bmec were isolated from a healthy lactating cow and characterized by their morphology, immunostaining for cytokeratin and the detection of beta- and kappa-casein mrnas. ten bacterial isolates comprising the staphylococcal species staphylococcus aureus (3), staphylococcus e ... | 2006 | 16624358 |
| dna microarray for detection of macrolide resistance genes. | a dna microarray was developed to detect bacterial genes conferring resistance to macrolides and related antibiotics. a database containing 65 nonredundant genes selected from publicly available dna sequences was constructed and used to design 100 oligonucleotide probes that could specifically detect and discriminate all 65 genes. probes were spotted on a glass slide, and the array was reacted with dna templates extracted from 20 reference strains of eight different bacterial species (streptococ ... | 2006 | 16723563 |
| interactions between glycopeptides and beta-lactams against isogenic pairs of teicoplanin-susceptible and -resistant strains of staphylococcus haemolyticus. | four isogenic derivatives with stably increased glycopeptide mics (all become resistant to teicoplanin) were obtained from four glycopeptide-susceptible clinical isolates of staphylococcus haemolyticus. all strains were extensively analyzed and compared for a number of distinctive features. in particular, the results provided insights into the puzzling issue of antistaphylococcal interactions between glycopeptides and beta-lactams, especially the paradox of double zones around beta-lactam disks ... | 2006 | 16801450 |
| reiter's syndrome and pelvic inflammatory disease in a couple. | | 2006 | 16836507 |
| automated systems in the identification and determination of methicillin resistance among coagulase negative staphylococci. | coagulase-negative staphylococci (cons) are an important cause of nosocomial bacteremia, specially in patients with indwelling devices or those submitted to invasive medical procedures. the identification of species and the accurate and rapid detection of methicillin resistance are directly dependent on the quality of the identification and susceptibility tests used, either manual or automated. the objective of this study was to evaluate the accuracy of two automated systems--microscan and vitek ... | 2006 | 16862322 |
| applications of lightcycler staphylococcus m-grade assay to detect staphylococcus aureus and coagulase-negative staphylococci in clinical blood samples and in blood culture bottles. | we evaluated the applicability of the lightcycler staphylococcus m(grade0 assay on artificially infected blood samples from healthy donors and on clinical specimens of 31 hospitalized patients. the sensitivity and specificity of the assay for detecting staphylococcus aureus was 100% in blood samples, and 100% in blood culture bottles, when data from the bactec 9120 blood culture system were taken as gold standard. the same specificity and sensitivity was found during the search for cons (coagula ... | 2006 | 16891851 |
| clinical significance of coagulase-negative staphylococci recovered from nonsterile sites. | laboratory criteria were used to select coagulase-negative staphylococci isolated from nonsterile body sites for further investigation. fifty-seven percent of the study isolates were clinically significant, predominantly causing community-acquired soft tissue infections. there were species-related differences in virulence, with 91% of staphylococcus lugdunensis isolates clinically significant compared with 11% of s. haemolyticus isolates. | 2006 | 16954288 |
| [retroperitoneal abscess in a man with severe necrotizing pancreatitis]. | history and admission findings: a 65-year-old man was admitted to the emergency unit of another hospital with severe acute abdominal pain. medial laparotomy revealed a tear in the lower pole of the capsule of the enlarged spleen. results of some laboratory tests became available only after the procedure: lipase and amylase levels were markedly elevated at 5097 u/l (normal range < 100 u/l) and 1776 u/l (normal range < 60 u/l), respectively. these findings suggested acute pancreatitis as cause of ... | 2006 | 16967391 |
| a new evolutionary variant of the streptogramin a resistance protein, vga(a)lc, from staphylococcus haemolyticus with shifted substrate specificity towards lincosamides. | we found a new variant of the streptogramin a resistance gene, vga(a)lc, in clinical isolates of staphylococcus haemolyticus resistant to lincomycin and clindamycin but susceptible to erythromycin and in which no relevant lincosamide resistance gene was detected. the gene vga(a)lc, differing from the gene vga(a) at the protein level by seven amino acid substitutions, was present exclusively in s. haemolyticus strains resistant to both lincosamides and streptogramin a (ls(a) phenotype). antibioti ... | 2006 | 17015629 |
| linezolid therapy of bloodborne teicoplanin-resistant staphylococcus haemolyticus. | | 2006 | 17033757 |
| staphylococcus hemolyticus liver abscess as an uncommon presentation of silent colonic cancer: a case report. | | 2006 | 17038083 |
| dna fingerprinting of a multi-resistant coagulase-negative staphylococci isolated from biomaterials in dialysis services. | coagulase-negative staphylococci (cons) have emerged as an important opportunistic pathogen isolated in hospital. several species of cons have been implicated in human infections and disease especially in patients with poor health status. | 2006 | 17045110 |
| fusidic acid resistance, mediated by fusb, in bovine coagulase-negative staphylococci. | the aim of this study was to determine the occurrence of fusidic acid resistance, mediated by the fusb gene, among coagulase-negative staphylococci (cons) isolated from bovine mastitis. | 2006 | 17060340 |
| antimicrobial sensitivity and adherence study in strains of coagulase-negative staphylococcus spp. | antimicrobial sensitivity was evaluated from 120 strains of coagulase-negative staphylococcus (cons), isolated from urinary infections. the isolated species were identified by conventional methods and sensitivity to vancomycin, tetracyclin, norfloxacin, cephalothin erythromycin, clindamycin, oxacillin, penicillin, cyprofloxacin, ampicillin and ampicillin/sulbactam was tested by the agar dilution technique and the diffusion in disc technique. the distribution of the species was: staphylococcus ep ... | 2001 | 17061495 |
| clonality of slime-producing methicillin-resistant coagulase-negative staphylococci disseminated in the neonatal intensive care unit of a university hospital. | methicillin-resistant coagulase-negative staphylococci (mr-cns) (n = 132), isolated from pre-term neonates, were analysed to determine their antibiotic resistance patterns, clonal distribution, biofilm production and the presence of the ica operon. all mr-cns were multiresistant, and 89% produced slime. a major clone was identified (77 isolates) among 115 staphylococcus epidermidis isolates. ten of 16 staphylococcus haemolyticus isolates also belonged to a single clone. most (80%) slime-positive ... | 2006 | 17121632 |
| staphylococcus haemolyticus endocarditis: clinical and microbiologic analysis of 4 cases. | only 3 cases of infective endocarditis (ie) due to methicillin-resistant staphylococcus haemolyticus (mrsh) have been reported in english literature. here we report 4 cases of ie due to mrsh encountered in a single university hospital. population analysis of the strains was performed to assess the presence of vancomycin/teicoplanin heteroresistant subpopulations. pulsed-field gel electrophoresis was used for molecular typing of isolates. ie was defined in 3 cases as health care associated, and i ... | 2007 | 17141458 |
| 3-methyl-3-sulfanylhexan-1-ol as a major descriptor for the human axilla-sweat odour profile. | this study sets out to redress the lack of knowledge in the area of volatile sulfur compounds (vscs) in axillary sweat malodour. sterile odourless underarm sweat (500 ml) was collected from 30 male volunteers after excessive sweating. five strains of bacteria, corynebacterium tuberculostearicum, corynebacterium minutissimum, staphylococcus epidermidis, staphylococcus haemolyticus, and bacillus licheniformis, were isolated and characterised for their ability to generate an authentic axillary odou ... | 2004 | 17191896 |
| identification of the precursor of (s)-3-methyl-3-sulfanylhexan-1-ol, the sulfury malodour of human axilla sweat. | a careful study of human axillary microflora led us to the identification of a new strain of staphylococcus haemolyticus. the role in axillary malodour formation of this microorganism was compared to those of corynebacterium xerosis and staphylococcus epidermidis, upon incubation on sterile human eccrine and apocrine axilla sweat. st. haemolyticus was responsible for the strongest sulfury malodour and the generation of the volatile sulfur compound (vsc) (s)-3-methyl-3-sulfanylhexan-1-ol (3). in ... | 2005 | 17192014 |
| precise excision and self-integration of a composite transposon as a model for spontaneous large-scale chromosome inversion/deletion of the staphylococcus haemolyticus clinical strain jcsc1435. | large-scale chromosomal inversions (455 to 535 kbp) or deletions (266 to 320 kbp) were found to accompany spontaneous loss of beta-lactam resistance during drug-free passage of the multiresistant staphylococcus haemolyticus clinical strain jcsc1435. identification and sequencing of the rearranged chromosomal loci revealed that issha1 of s. haemolyticus is responsible for the chromosome rearrangements. | 2007 | 17237177 |
| multiple staphylococcal cassette chromosomes and allelic variants of cassette chromosome recombinases in staphylococcus aureus and coagulase-negative staphylococci from norway. | we investigated the nature of the staphylococcal cassette chromosome mec (sccmec) elements and cognate insertion sites in a collection of 42 clinical staphylococcal isolates of various species from norway. the ccr and mec genes and the attachment sites (attl/attr) were identified by pcr, southern blot hybridization, and dna sequencing. we found 10 possibly new sccmec types and one previously unreported variant of sccmec type iii (mec complex a, ccrab3, and ccrc7) in staphylococcus epidermidis, s ... | 2007 | 17307983 |
| in vitro activity of antimicrobial agents against oxacillin resistant staphylococci with special reference to staphylococcus haemolyticus. | one hundred and sixty seven isolates of staphylococci isolated from the inpatients of a tertiary care referral hospital in south india were speciated and activity of oxacillin, glycopeptides, linezolid and quinupristin/dalfopristin against these isolates was tested by broth microdilution method. of the 114 coagulase negative staphylococci (cons), 49.1 % were s. haemolyticus, isolated predominantly from urine (64.6%), while the rest belonged to 11 other species. more than half the isolates of s. ... | 2007 | 17377353 |
| [infection of severe burns. new perspectives of treatment in primary care]. | | 2007 | 17380632 |
| bacteria trigger oxygen radical release and sperm lipid peroxidation in in vitro model of semen inflammation. | to assess the in vitro effect of five bacterial strains isolated from semen samples (escherichia coli, staphylococcus haemolyticus, streptococcus oralis, bacteroides ureolyticus, and ureaplasma urealyticum) on reactive oxygen intermediate (roi) release and lipid sperm membrane peroxidation in the coincubated suspensions of white blood cells (wbc) with spermatozoa. | 2007 | 17383646 |
| [molecular study of methicillin-resistant staphylococcus haemolyticus in a mexican hospital]. | to perform the molecular characterization of methicillin-resistant staphylococcus haemolyticus (mrsh) clinical isolates from patients in a mexican hospital. | 2006 | 17432290 |
| staphplex system for rapid and simultaneous identification of antibiotic resistance determinants and panton-valentine leukocidin detection of staphylococci from positive blood cultures. | phenotypic methods take several days for identification and antimicrobial susceptibility testing of staphylococcal isolates after gram-positive cocci in clusters (gpcc) are observed in positive blood cultures. we developed and validated a staphplex system that amplifies and detects 18 gene targets simultaneously in 1 reaction for species-level identification of staphylococci, detection of genes encoding panton-valentine leukocidin (pvl), and antimicrobial resistance determinants of staphylococci ... | 2007 | 17446323 |
| characterisation of staphylococcus haemolyticus isolated from urinary tract infections. | to determine some virulence factors and antimicrobial susceptibilities of staphylococcus haemolyticus from urinary tract infections. | 2005 | 17447342 |
| antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey. | the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ... | 2009 | 17475456 |
| distribution and expression of macrolide resistance genes in coagulase-negative staphylococci. | in total, 494 isolates of coagulase-negative staphylococci (cons) were identified to the species level by biochemical tests and soda sequencing. erythromycin resistance phenotypes were determined and specific resistance genes were identified by pcr. the prevalence of erythromycin resistance varied widely among staphylococcal species, from 0% in staphylococcus lugdunensis to almost 90% in staphylococcus haemolyticus. most (63%) erythromycin-resistant isolates carried constitutively expressed erm( ... | 2007 | 17501977 |
| detection of vancomycin heteroresistant staphylococcus haemolyticus and vancomycin intermediate resistant staphylococcus epidermidis by means of vancomycin screening agar. | | 2007 | 17558874 |
| [staphylococcus haemolyticus with reduced susceptibility to vancomycin isolated from a patient with neonatal meningitis in a mexican medical facility]. | reduced susceptibility to vancomycin has been reported among clinical isolates of staphylococcus in previous studies. the present study describes a heteroresistant staphylococci strain from the cerebrospinal fluid of a 17 day-old premature male infant with neonatal meningitis. screening was done for 44 strains of coagulase-negative staphylococci using wong 's techniques (disk-agar method with vancomycin-salt agar to demonstrate satellitism around an aztreonam disk). strains were susceptible to v ... | 2007 | 17585704 |
| first report in spain of linezolid non-susceptibility in a clinical isolate of staphylococcus haemolyticus. | | 2007 | 17600688 |
| novel 10-bp deletion in the translational attenuator of a constitutively expressed erm(a) gene from staphylococcus epidermidis. | alterations in the erm(a) regulatory region of six clinical isolates of staphylococcus epidermidis and one of staphylococcus haemolyticus displaying a constitutive resistance phenotype were investigated. anovel deletion of 10 bp with respect to the corresponding sequence of tn554 was identified in the attenuator of a constitutively expressed erm(a) gene of one of the s. epidermidis isolates. thus far, this is the smallest deletion conferring constitutive resistance in the translational attenuato ... | 2007 | 17661294 |
| differentiation of staphylococcus spp. by terminal-restriction fragment length polymorphism analysis of glyceraldehyde-3-phosphate dehydrogenase-encoding gene. | classical phenotypic and biochemical testing do not lead to correct identification of the distinct staphylococcus species. therefore, the aim of our study was to develop a method for the reliable and accurate determination of distinct staphylococcus species. in the present study, the 931-934-bp partial sequences of the glyceraldehyde-3-phosphate dehydrogenase-encoding (gap) gene of 28 validly described staphylococcus species were amplified and sequenced. by using the respective sequence informat ... | 2007 | 17681623 |
| in vitro activity of daptomycin and tigecycline against coagulase-negative staphylococcus blood isolates from bone marrow transplant recipients. | multi-resistant coagulase-negative staphylococci (cns) may cause systemic infections in patients undergoing bone marrow transplantation. daptomycin, a new lipopeptide, and tigecycline, a new glycylcycline, have excellent activity against gram-positive bacteria including methicillin-resistant staphylococci. this study presents the in vitro activity of daptomycin and tigecycline compared to vancomycin and fosfomycin against 105 cns isolated from 76 bone marrow transplant patients with symptomatic ... | 2007 | 17714506 |
| persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: virulence factors and invasiveness. | coagulase-negative staphylococci (cons) are the major cause of nosocomial bacteraemia in neonates. the aim of this study was to investigate whether persistent strains of cons possess specific bacterial characteristics as compared with sporadic non-cluster isolates. in total, 180 blood culture isolates (95 contaminants and 85 invasive isolates) obtained from a single neonatal unit over a 12-year period were studied. pulsed-field gel electrophoresis (pfge) identified 87 persistent cons strains (en ... | 2007 | 17850346 |
| evolution of slime production by coagulase-negative staphylococci and enterotoxigenic characteristics of staphylococcus aureus strains isolated from various human clinical specimens. | the present study was designed to determine the slime production of coagulase-negative staphylococci (cons) and the enterotoxigenic properties of staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing cons and enterotoxigenic s. aureus strains isolated from various human clinical specimens. for this purpose, a total of 120 staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. of the cli ... | 2007 | 17893164 |