| permeabilization of transformed cells in culture by external atp. | | 2013 | 3900411 |
| [lessons from the families through the care of hospitalized patients]. | | 2013 | 3637587 |
| compliance in contact lens care. | fifty patients who were originally fitted or refitted with daily-wear contact lenses at the ucla contact lens service were interviewed on the nature of their care system compliance and hygiene. good compliance was defined as meeting three preset, standardized criteria (hand washing before lens manipulation, correct use of a fda-approved care system, and adherence to a daily-wear schedule). twenty of our patients, or 40%, were found to be noncompliant. noncompliance occurred more frequently in th ... | 2013 | 3591899 |
| hypertension in childhood. | | 1998 | 90925 |
| measurement of antihaemophilic factor a antigen (vii:cag) with a solid phase immunoradiometric method based on homologous non-haemophilic antibodies. | antihaemophilic-factor-a-antibodies, which had spontaneously arisen in 2 patients, were used to develop an immunoradiometric method for measurement of antihaemophilic factor a antigen (viii:cag). 13 patients with severe haemophilia a had vii:cag below the limit of detection (0.01 u/ml). patients with moderate and mild haemophilia a either had vii:cag roughly equal to factor viii clotting activity (viii:c) or a not detectable vii:cag, suggesting 2 different molecular mechanisms in moderate and mi ... | 1999 | 91190 |
| retrocaecal peri-appendicular abscess simulating right ureterolithiasis. | | 2012 | 3349288 |
| immune disorders of the gastrointestinal tract and liver. | immune disorders of the gastrointestinal tract and hepatobiliary systems comprise a diverse group of illnesses which share in common certain overlapping and yet distinctive expressions of cellular and humoral immunity. as is evident from material contained in this article, controversy and disparate results frequently characterize the study of immune mechanisms in a given disease process. nonetheless, advances in quantitation of specific immunocyte function and phenotypic expression have greatly ... | 2013 | 3903375 |
| immune disorders of the gastrointestinal tract and liver. | immune disorders of the gastrointestinal tract and hepatobiliary systems comprise a diverse group of illnesses which share in common certain overlapping and yet distinctive expressions of cellular and humoral immunity. as is evident from material contained in this article, controversy and disparate results frequently characterize the study of immune mechanisms in a given disease process. nonetheless, advances in quantitation of specific immunocyte function and phenotypic expression have greatly ... | 2013 | 3903375 |
| bilateral double nipples. | | 2016 | 5007671 |
| [diagnostic significance of the dissociation quotient in the hamburg-wechsler intelligence test]. | | 1996 | 177897 |
| [ways of practical solution of socio-hygienic problems of reducing infant mortality]. | | 2000 | 2144620 |
| tryptophan-nicotinamide combination in the treatment of newly admitted depressed patients. | | 2003 | 153217 |
| symptomatic carotid artery stenosis: a solvable problem. north american symptomatic carotid endarterectomy trial. | | 2006 | 1636175 |
| [cryptosporidiosis. characterization of a new infection with special regard to water as the source of infection]. | within the past few years, microorganisms of the genus cryptosporidium have been recognized as an important pathogen to humans. a possible mode of transmission is represented by the presence of cryptosporidium-oocysts in water. meanwhile several outbreaks of diarrheal illness caused by the contamination of drinking water by cryptosporidium have been documented; an infection can be life-threatening for immunosuppressed patients. this article reviews the organism causing cryptosporidiosis, his eco ... | 2007 | 2203371 |
| [quality control in dental radiography]. | | 2008 | 2639024 |
| helicopter emergency medical services--hems one. | | 2009 | 2774466 |
| [severe dysphagia in a woman with chronic symptomatic gastroesophageal reflux]. | | 2009 | 2772336 |
| folk psychology and talking hyoids. | | 2009 | 2685609 |
| [clinico-etiologic characteristics of severe and complicated forms of acute respiratory virus infections and their treatment]. | | 2010 | 2951902 |
| [food ingestion in ruminants: modalities and associated phenomena]. | this paper synthesizes the literature on modalities of food intake in ruminants and their main associated phenomena. firstly, circadian distribution of feeding and ruminating activities has been examined. ruminants spend a large part of their time chewing. their meals have been described in detail; changes in rates of intake, time spent eating, the effect of restricting the amount fed or the period of feed accessibility have been discussed. when food is distributed, the animals have a "long" mea ... | 2010 | 3550977 |
| [changing nursing profession and related matters reflected in statistics. 6. reemployment of nurses]. | | 2011 | 3185003 |
| [toward new boundaries of public health]. | | 2011 | 3323662 |
| some views on the influence of morphine on brain stem pain modulating neurons and descending controls acting on the spinal cord. | | 2011 | 3064170 |
| dreams during language learning: when and how is the new language integrated. | | 2011 | 3043514 |
| [functional differentiation of thymocytes induced by macrophages: cellular, humoral and genetic aspects]. | the cellular, humoral and genetical mechanisms of induction of t-effectors of the graft vs. host reaction (ghr) were studied in a double-cell culture of phagocytizing mononuclears with thymocytes. experiments were carried out on mice of inbred and recombinant strains. the ghr intensity was estimated by the increase in the number of cells in the popliteal lymph node, regional with reference to the introduction of parental thymocytes into the f1 hybrid. for the induction of the ghr t-effectors fro ... | 2012 | 3486393 |
| [basic and clinical studies on the measurement of serum ca 19-9]. | | 2012 | 3463815 |
| physical treatment planning of total body irradiation. | the treatment planning of total body irradiations shows partially other problems than conventional treatments. the data refer to long source-skin-distance and large fields, which are even larger than the patient. in most centres, it is aspired to a homogeneous dose distribution. but its importance is not known, at present. the radiobiology has to yield further answers to a lot of questions. this and the clinical results must help to decide, which technique is the best to eliminate leukemic cells ... | 2012 | 3518105 |
| downwind update--a discourse on matters gaseous. | | 2012 | 3538662 |
| [long-term therapy of multiple piloleiomyomas with nifedipine]. | we report on a 46-year-old female patient suffering from painful disseminated cutaneous piloleiomyomata on her left leg. treatment with nifedipine over 3 years resulted in suppressing the pain almost totally. | 2012 | 3433859 |
| [basic trends in propagandizing medical knowledge today]. | | 2012 | 3356258 |
| growth stimulating activity of lung extract on lung-colonizing colon 26 clones and its partial characterization. | the effects of lung tissue extract on the cell growth of eight colon 26 tumor clones, four highly and four poorly lung-colonizing clones, were examined in vitro. addition of lung extract to serum-free medium stimulated the growth of all four of the highly lung-colonizing clones and one of the poorly lung-colonizing clones, but it had minimal effect on the other three poorly lung-colonizing clones. these results indicate that the lung extract contains a growth stimulating activity; it selectively ... | 2012 | 3345612 |
| prevention of posttransplant acute tubular necrosis by the calcium antagonist diltiazem: a prospective randomized study. | in a prospective randomized trial we evaluated the influence of the calcium antagonist diltiazem (dil) on the development of acute tubular necrosis (atn) in cadaveric kidney transplantation. dil was added to eurocollin's solution (20 mg/l) at donor nephrectomy. the graft recipient received a preoperative bolus injection of dil (0.28 mg/kg) which was followed by an infusion of dil (0.0022 mg/min/kg) for 2 days. thereafter, dil was applied orally. immunosuppressive therapy consisted of ciclosporin ... | 2012 | 3318461 |
| an improved simple procedure for the determination of thiamine in food. | a modified fluorometric method for the determination of thiamine in food is described. the procedure is simple, reproducible, does not demand costly outfit and chemicals and is not susceptible to interference. the recovery of added thiamine is good. | 2013 | 3748130 |
| [promises of job will attract youth]. | | 2013 | 3651084 |
| [the control of lysozyme synthesis in temperate bacteriophages]. | | 2014 | 4168904 |
| lung fixation by perfusion--a simple method to control the pressure in the perfusion circuit. | | 2014 | 4597874 |
| enzymo-chemical identification of leukemic cells. | | 2014 | 4184580 |
| reviews in cardiology. a cooperative program of the american college of cardiology and the national library of medicine. | | 2014 | 4597819 |
| [normal values of the biochemical components of sera collected from the umbilical artery, umbilical vein and maternal vein]. | | 2014 | 4375218 |
| [normal values of the biochemical components of sera collected from the umbilical artery, umbilical vein and maternal vein]. | | 2014 | 4375218 |
| [diagnosis and surgical treatment of esophageal cysts]. | | 2015 | 4432338 |
| [ceporex 250. ceporex 500]. | | 2015 | 4408781 |
| microevolutionary change in arikara crania: a multivariate analysis. | | 2015 | 4682526 |
| [therapy of pulmonary suppurations]. | | 2016 | 5043556 |
| hospital matrons--unlimited hours--appeal judgment of the industrial court. | | 2016 | 4973939 |
| industrial nail-gun injuries: a review and case report. | | 2016 | 4742526 |
| the cyclic antibacterial peptide enterocin as-48: isolation, mode of action, and possible food applications. | enterocin as-48 is a circular bacteriocin produced by enterococcus. it contains a 70 amino acid-residue chain circularized by a head-to-tail peptide bond. the conformation of enterocin as-48 is arranged into five alpha-helices with a compact globular structure. enterocin as-48 has a wide inhibitory spectrum on gram-positive bacteria. sensitivity of gram-negative bacteria increases in combination with outer-membrane permeabilizing treatments. eukaryotic cells are bacteriocin-resistant. this catio ... | 2014 | 25493478 |
| bacterial dynamics during yearlong spontaneous fermentation for production of ngari, a dry fermented fish product of northeast india. | ngari is the most popular traditionally processed non-salted fish product, prepared from sun-dried small cyprinid fish puntius sophore (ham.) in manipur state of northeast india. the microbial involvement in ngari production remained uncertain due to its low moisture content and yearlong incubation in anaerobically sealed earthen pots without any significant change in total microbial count. the culture-independent pcr-dgge analysis used during this study confirmed a drastic bacterial community s ... | 2015 | 25637876 |
| evaluation of bacterial flora during the ripening of kedong sufu, a typical chinese traditional bacteria-fermented soybean product. | kedong sufu is a typical bacteria-fermented sufu in china. isolation and identification of the autochthonous bacteria involved would allow the design of specific starters for this speciality. the purpose of the present study was to evaluate the bacterial flora during the ripening of kedong sufu using polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge) and culturing. | 2013 | 23400969 |
| identification of microbiota present on the surface of taleggio cheese using pcr-dgge and rapd-pcr. | microbial dna from 9 batches of taleggio pdo cheese sampled at various times during ripening, brines, swabs of wooden shelves used for cheese dry-salting, and 13 commercial cheeses were analyzed by denaturing gradient gel electrophoresis (pcr-dgge) and/or random amplification of polymorphic dna (rapd-pcr). sequencing allowed the detection of 12 genera, 27 species, and 2 unclassified bacteria. molecular analysis allowed for the detection of microorganisms not previously associated with taleggio s ... | 2012 | 23094819 |
| s-bacillithiolation protects conserved and essential proteins against hypochlorite stress in firmicutes bacteria. | protein s-bacillithiolations are mixed disulfides between protein thiols and the bacillithiol (bsh) redox buffer that occur in response to naocl in bacillus subtilis. we used bsh-specific immunoblots, shotgun liquid chromatography (lc)-tandem mass spectrometry (ms/ms) analysis and redox proteomics to characterize the s-bacillithiolomes of b. subtilis, b. megaterium, b. pumilus, b. amyloliquefaciens, and staphylococcus carnosus and also measured the bsh/oxidized bacillithiol disulfide (bssb) redo ... | 2013 | 22938038 |
| effect of commercial starter cultures on volatile compound profile and sensory characteristics of dry-cured foal sausage. | the present work deals with the evaluation of the effect of three different commercial starter cultures (chr. hansen, hørsholm, denmark) on the volatile compound profile and sensory properties, as well as some important physicochemical parameters, of dry-fermented foal sausages at the end of ripening in order to select the most suitable starter culture for this elaboration. the sausage batches were named as follows: co (non-inoculated control), fs (lactobacillus sakei + staphylococcus carnosus), ... | 2016 | 25851258 |
| development of pla films containing oregano essential oil (origanum vulgare l. virens) intended for use in food packaging. | consumers' concerns about the environment and health have led to the development of new food packaging materials avoiding petroleum-based matrices and synthetic additives. the present study has developed polylactic acid (pla) films containing different concentrations of essential oil from origanum vulgare l. virens (oeo). the effectiveness of this new active packaging was checked for use in ready-to-eat salads. a plasticising effect was observed when oeo was incorporated in pla films. the rest o ... | 2016 | 27455176 |
| anti-mutagenic and immuno-stimulatory properties of lactic acid bacteria. | statistically significant antigenotoxic activity was exerted by six of nine strains of lactic acid bacteria tested (lactobacillus delbrueckii subsp. bulgaricus, staphylococcus carnosus, streptococcus thermophilus, l. rhamnosus, enterococcus faecium and en. faecalis) against nitrovin and 2-aminofluorene in salmonella typhimurium ta100 and ta97. the mutagenic activity of both mutagens was substantially decreased by viable bacteria; cells heated to 100°c for 15 min were ineffective. in vitro, en. f ... | 1995 | 24414651 |
| influence of application sequence and timing of eugenol and lauric arginate (lae) on survival of spoilage organisms. | the effectiveness of sequential applications of the antimicrobials eugenol and lauric arginate (lae) was investigated against staphylococcus carnosus, listeria innocua, escherichia coli k12, and pseudomonas fluorescens. the antimicrobials were applied simultaneously at half of their minimum lethal concentrations (mlc) or sequentially at t = 0 h and t = 3, 4, 6 or 8 h. bacterial survival was determined by direct plate counts. survivals kinetic were fitted to a growth and mortality model to obtain ... | 2017 | 28213028 |
| antimicrobial efficacy of sequentially applied eugenol against food spoilage micro-organisms. | compare the survival behaviour of food spoilage micro-organisms treated with sequential doses or all at once treatments of eugenol. | 2016 | 27626598 |
| nematicidal and antimicrobial activities of methanol extracts of 17 plants, of importance in ethnopharmacology, obtained from the arabian peninsula. | the development of resistance to synthetic drugs by target organisms is a major challenge facing medicine, yet locked within plants are phytochemicals used in herbal medicine (especially in the arabian peninsula) that may find application in this regard. in pursuit of unlocking these "hidden treasures," the methanol extracts of leaves, aerial parts, fruits, and resins of 17 plants used in the arabian peninsula were screened for antimicrobial activities. | 2017 | 27104031 |
| natural selenium particles from staphylococcus carnosus: hazards or particles with particular promise? | various bacteria, including diverse staphylococci, reduce selenite to yield red selenium particles with diameters in the high nanometer to low micrometer range. formation and accumulation of such particles in bacteria often results in cell death, triggered by a loss of thiols and formation of disruptive deposits inside the cell. hence certain pathogenic bacteria are rather sensitive to the presence of selenite, whilst other organisms, such as small nematodes, do not employ this kind of nanotechn ... | 2017 | 26897703 |
| survival of spoilage bacteria subjected to sequential eugenol and temperature treatments. | effects of a sequential application of eugenol and temperature on the survival of two model spoilage organisms, staphylococcus carnosus lth1502 and escherichia coli k12 c600, were studied. to assess effects of a "temperature first-antimicrobial later" treatment, cultures were treated with eugenol at 20, 37 and 42 °c at the beginning of the incubation period, and after 3h and 8h. to assess effects of an "antimicrobial first-temperature later" treatment, eugenol was added at the beginning of the i ... | 2016 | 26590802 |
| thermal tolerance of o157 and non-o157 shiga toxigenic strains of escherichia coli, salmonella, and potential pathogen surrogates, in frankfurter batter and ground beef of varying fat levels. | the non-o157 shiga toxigenic escherichia coli (stec) serogroups most commonly associated with illness are o26, o45, o103, o111, o121, and o145. we compared the thermal tolerance (d55°c) of three or more strains of each of these six non-o157 stec serogroups with five strains of o157:h7 stec in 7% fat ground beef. d55°c was also determined for at least one heat-tolerant stec strain per serogroup in 15 and 27% fat ground beef. d55°c of single-pathogen cocktails of o157 and non-o157 stec, salmonella ... | 2014 | 25198841 |
| impact of fatty acid chain length of rosmarinate esters on their antimicrobial activity against staphylococcus carnosus lth1502 and escherichia coli k-12 lth4263. | the effect of the addition of a newly synthesized series of rosmarinic acid (ra) estes (res) and alcohols with chain lengths of 1, 4, 8, 10, 12, 16, and 18 carbons (re1 to 18) on the growth behavior of staphylococcus carnosus lth1502 and escherichia coli k-12 lth4263 was investigated. an initial microtiter dilution assay indicated activity of compounds against s. carnosus lth1502, whereas esters with chain lengths, ra, n-methyl rosmarinate (re1), n-dodecyl rosmarinate (re12), and n-octadecyl ros ... | 2013 | 23992498 |
| characterization of a novel n-acetylneuraminate lyase from staphylococcus carnosus tm300 and its application to n-acetylneuraminic acid production. | the possibility of incorporating n-acetylneuraminic acid (neu5ac) in infant formulas and other functional foods has opened up the need to synthesize n-acetylneuraminic acid using n-acetylneuraminate lyases (nals) by reversible aldol condensation of pyruvate and n-acetyl-d-mannosamine. until now, nals have been cloned from pathogenic microorganisms; however, this article describes the expression and characterization of an n-acetylneuraminate lyase from the staphylococcus carnosus tm300, a gras mi ... | 2012 | 22803763 |
| functional expression and extension of staphylococcal staphyloxanthin biosynthetic pathway in escherichia coli. | the biosynthetic pathway for staphyloxanthin, a c(30) carotenoid biosynthesized by staphylococcus aureus, has previously been proposed to consist of five enzymes (crto, crtp, crtq, crtm, and crtn). here, we report a missing sixth enzyme, 4,4'-diaponeurosporen-aldehyde dehydrogenase (aldh), in the staphyloxanthin biosynthetic pathway and describe the functional expression of the complete staphyloxanthin biosynthetic pathway in escherichia coli. when we expressed the five known pathway enzymes thr ... | 2012 | 22535955 |
| phosphate starvation as an antimicrobial strategy: the controllable toxicity of lanthanum oxide nanoparticles. | lanthanum oxide nanoparticles were utilized to scavenge phosphate from microbial growth media for the use of targeted nutrient starvation as an antimicrobial strategy. only in phosphate poor environments a toxic effect was observed. the effect was shown on escherichia coli, staphylococcus carnosus, penicillium roqueforti, and chlorella vulgaris. | 2012 | 22410707 |
| chemical characterisation and histamine-forming bacteria in salted mullet roe products. | sixteen salted mullet roe products sold in the retail markets in taiwan were purchased and tested to determine the occurrence of histamine and histamine-forming bacteria. the levels of ph, salt content, water content, total volatile basic nitrogen (tvbn) and aerobic plate count (apc) in all samples ranged from 5.4 to 5.8, 5.1% to 7.2%, 15.4% to 27.3%, 32.0 to 69.6mg/100g and <1.0 to 7.1logcfu/g, respectively. none of these samples contained total coliform and escherichia coli. the average conten ... | 2008 | 26049242 |
| application of autochthonous mixed starter for controlled kedong sufu fermentation in pilot plant tests. | traditional sufu is fermented by back-slopping and back-slopping has many defects. the objective of this study was to apply autochthonous mixed starter to control kedong sufu fermentation. sufu was manufactured using back-slopping (batch a) and autochthonous mixed starter (batch b) with kocuria kristinae f7, micrococcus luteus kdf1, and staphylococcus carnosus kdfr1676. considering physicochemical properties of sufu, 150-day sufu samples from batch a and 90-day sufu samples from batch b met the ... | 2015 | 25533352 |
| degradation of pcbs in dry fermented sausages during drying/ripening. | the effects of several commercial meat starter cultures on degradation of polychlorinated biphenyls (pcbs) in dry fermented sausages over 28days of drying/ripening were investigated. the sausage batter was prepared according to a classic recipe and spiked with a standard solution of a pcb congener mixture. with addition of different commercial meat starter cultures, five experimental groups were prepared: no further addition; and separate addition of each of four starter cultures: texel dcm-1, t ... | 2016 | 27451178 |
| process-driven bacterial community dynamics are key to cured meat colour formation by coagulase-negative staphylococci via nitrate reductase or nitric oxide synthase activities. | the cured colour of european raw fermented meats is usually achieved by nitrate-into-nitrite reduction by coagulase-negative staphylococci (cns), subsequently generating nitric oxide to form the relatively stable nitrosomyoglobin pigment. the present study aimed at comparing this classical curing procedure, based on nitrate reductase activity, with a potential alternative colour formation mechanism, based on nitric oxide synthase (nos) activity, under different acidification profiles. to this en ... | 2015 | 25805616 |
| effect of starter culture and turmeric on physico-chemical quality of carabeef pastirma. | carabeef samples were sliced, pressed, cured and divided into 6 groups. starter cultures (micrococcus varians m483 (mv), staphylococcus carnosus (sc), lactobacillus sakei (ls), m. varians m483+ lb. sakei and staph. carnosus + lb. sakei) were inoculated at the dose of 10(6)-0(7)cfu/g and stored at 10 ± 1°c for 7 days. uninoculated samples were maintained as control. samples were then divided into 2 treatment groups. samples of treatment 1 (t1) were smeared with a paste of turmeric followed by app ... | 2010 | 23572607 |
| the branched-chain amino acid aminotransferase encoded by ilve is involved in acid tolerance in streptococcus mutans. | the ability of streptococcus mutans to produce and tolerate organic acids from carbohydrate metabolism represents a major virulence factor responsible for the formation of carious lesions. pyruvate is a key metabolic intermediate that, when rerouted to other metabolic pathways such as amino acid biosynthesis, results in the alleviation of acid stress by reducing acid end products and aiding in maintenance of intracellular ph. amino acid biosynthetic genes such as ilvc and ilve were identified as ... | 2012 | 22328677 |
| identifying ingredients that delay outgrowth of listeria monocytogenes in natural, organic, and clean-label ready-to-eat meat and poultry products. | the objective of this study was to identify ingredients that inhibit listeria monocytogenes in natural, organic, or clean-label ready-to-eat meat and poultry products. fourteen ingredients were screened in uncured (no-nitrate-or-nitrite-added), traditional-cured (156 ppm of purified sodium nitrite), cultured (alternative cured, natural nitrate source, and staphylococcus carnosus), or preconverted (alternative cured, natural nitrite source) turkey slurries. slurries were cooked, cooled, inoculate ... | 2013 | 23905792 |
| transcriptomic analysis of staphylococcal srnas: insights into species-specific adaption and the evolution of pathogenesis. | next-generation sequencing technologies have dramatically increased the rate at which new genomes are sequenced. accordingly, automated annotation programs have become adept at identifying and annotating protein coding regions, as well as common and conserved rnas. additionally, rnaseq techniques have advanced our ability to identify and annotate regulatory rnas (srnas), which remain significantly understudied. recently, our group catalogued and annotated all previously known and newly identifie ... | 2016 | 28348860 |
| the peptidoglycan pattern of staphylococcus carnosus tm300-detailed analysis and variations due to genetic and metabolic influences. | the gram-positive bacterium staphylococcus carnosus (s. carnosus) tm300 is an apathogenic staphylococcal species commonly used in meat starter cultures. as with all gram-positive bacteria, its cytoplasmic membrane is surrounded by a thick peptidoglycan (pgn) or murein sacculus consisting of several layers of glycan strands cross-linked by peptides. in contrast to pathogenic staphylococci, mainly staphylococcus aureus (s. aureus), the chemical composition of s. carnosus pgn is not well studied so ... | 2016 | 27669322 |
| comparative genomic analysis of the genus staphylococcus including staphylococcus aureus and its newly described sister species staphylococcus simiae. | staphylococcus belongs to the gram-positive low g + c content group of the firmicutes division of bacteria. staphylococcus aureus is an important human and veterinary pathogen that causes a broad spectrum of diseases, and has developed important multidrug resistant forms such as methicillin-resistant s. aureus (mrsa). staphylococcus simiae was isolated from south american squirrel monkeys in 2000, and is a coagulase-negative bacterium, closely related, and possibly the sister group, to s. aureus ... | 2012 | 22272658 |
| prevalence of antibiotic resistance in coagulase-negative staphylococci from spontaneously fermented meat products and safety assessment for new starters. | to provide new meat starter strains lacking antibiotic (ab) resistances, we explored the ab susceptibility in 116 coagulase-negative staphylococcus (cns) isolates from traditionally fermented sausages (n=40) manufactured with meat from conventional animal breeding, and from meat products (n=76) made from meat of animals raised in natural habitats under low- or no-antibiotic pressure. less than 50% of these cns isolates showed phenotypic resistances to at least one antibiotic (ab) by using microd ... | 2012 | 23072691 |
| the use of nucleosides and arginine as alternative energy sources by coagulase-negative staphylococci in view of meat fermentation. | the ability of coagulase-negative staphylococci (cns) to use alternative energy sources in meat may partially explain their occurrence in fermented meats. of 61 cns strains tested, all metabolized adenosine and inosine in a meat simulation medium (msm). the ability to catabolize arginine via the arginine deiminase (adi) pathway varied between strains. all tested strains of staphylococcus carnosus and staphylococcus epidermidis possessed an arca gene and showed adi activity, whereas other species ... | 2014 | 24387852 |
| amino acid conversions by coagulase-negative staphylococci in a rich medium: assessment of inter- and intraspecies heterogeneity. | the ability of coagulase-negative staphylococci (cns) to convert amino acids into volatile compounds and biogenic amines was investigated after 24h and 48 h of incubation in a rich medium (brain heart infusion). volatile compounds were measured with static-headspace gas chromatography and mass spectrometry (sh-gc-ms); biogenic amine measurements were carried out with a newly developed method based on ultra-performance liquid chromatography coupled to tandem mass spectrometry (uplc-ms/ms). in tot ... | 2015 | 25991555 |
| accumulation-associated protein enhances staphylococcus epidermidis biofilm formation under dynamic conditions and is required for infection in a rat catheter model. | biofilm formation is the primary virulence factor of staphylococcus epidermidis. s. epidermidis biofilms preferentially form on abiotic surfaces and may contain multiple matrix components, including proteins such as accumulation-associated protein (aap). following proteolytic cleavage of the a domain, which has been shown to enhance binding to host cells, b domain homotypic interactions support cell accumulation and biofilm formation. to further define the contribution of aap to biofilm formatio ... | 2015 | 25332125 |
| characterization and technological features of autochthonous coagulase-negative staphylococci as potential starters for portuguese dry fermented sausages. | the manufacture of dry fermented sausages is an important part of the meat industry in southern european countries. these products are usually produced in small shops from a mixture of pork, fat, salt, and condiments and are stuffed into natural casings. meat sausages are slowly cured through spontaneous fermentation by autochthonous microbiota present in the raw materials or introduced during manufacturing. the aim of this work was to evaluate the technological and safety features of coagulase- ... | 2016 | 27095684 |
| unraveling the microbiota of teat apices of clinically healthy lactating dairy cows, with special emphasis on coagulase-negative staphylococci. | swab samples (n=72) obtained from the teat apex of lactating dairy cows without visual signs of inflammation (n=18) were gathered on 2 well-managed flemish dairy herds (herds 1 and 2) during the same month to assess the bacterial diversity of teat apices before milking. a combination of both culture-dependent [plating and (gtg)(5)-pcr fingerprinting of the colonies] and culture-independent [denaturing gradient gel electrophoresis (pcr-dgge)] techniques indicated that the teat apices contain a wi ... | 2013 | 23313004 |
| the n-terminal repeat and the ligand binding domain a of sdri protein is involved in hydrophobicity of s. saprophyticus. | staphylococcus saprophyticus is an important cause of urinary tract infection, and its cell surface hydrophobicity may contribute to virulence by facilitating adherence of the organism to uroepithelia. s. saprophyticus expresses the surface protein sdri, a member of the serine-aspartate repeat (sd) protein family, which has multifunctional properties. the sdri knock out mutant has a reduced hydrophobicity index (hpi) of 25%, and expressed in the non-hydrophobic staphylococcus carnosus strain tm3 ... | 2015 | 25497915 |
| prevalence of enterotoxin-encoding genes and antimicrobial resistance in coagulase-negative and coagulase-positive staphylococcus isolates from black pudding. | staphylococcal species are pathogens that are responsible for outbreaks of foodborne diseases. the aim of this study was to investigate the prevalence of enterotoxin-genes and the antimicrobial resistance profile in staphylococcus coagulase-negative (cons) and coagulasepositive (cops) isolates from black pudding in southern brazil. | 2012 | 23152340 |
| degradation of pcbs in a frankfurter-type meat emulsion: effects of a meat starter, its proteins extract and thermal treatments. | the degradation of a series of polychlorinated biphenyl (pcb) congeners (pcbs 10, 28, 52, 138, 153, 180) in meat emulsions of a frankfurter type was investigated. with a pool of these pcbs added to the meat emulsion, three initial experimental groups were used: no further addition, or plus a commercial meat starter (containing staphylococcus xylosus and staphylococcus carnosus), or plus the protein extract from this commercial meat starter. prior to further treatments, these samples were incubat ... | 2012 | 22659289 |
| enhanced adhesion of streptococcus mutans to hydroxyapatite after exposure to saliva. | streptococcus mutans cells form robust biofilms on human teeth and are strongly related to caries incidents. hence, understanding the adhesion of s. mutans in the human oral cavity is of major interest for preventive dentistry. in this study, we report on atomic force microscopy-based single-cell force spectroscopy measurements of s. mutans cells to hydroxyapatite surfaces. we observe for almost all measurements a significant difference in adhesion strength for s. mutans as well as for staphyloc ... | 2017 | 28256775 |
| reduced adhesion of oral bacteria on hydroxyapatite by fluoride treatment. | the mechanisms of action of fluoride have been discussed controversially for decades. the cavity-preventive effect for teeth is often traced back to effects on demineralization. however, an effect on bacterial adhesion was indicated by indirect macroscopic studies. to characterize adhesion on fluoridated samples on a single bacterial level, we used force spectroscopy with bacterial probes to measure adhesion forces directly. we tested the adhesion of streptococcus mutans , streptococcus oralis , ... | 2013 | 23556545 |
| protease substrate profiling using bacterial display of self-blocking affinity proteins and flow-cytometric sorting. | proteases are involved in fundamental biological processes and are important tools in both biotechnological and biomedical research. an important property of proteases is to discriminate among potential substrates. here, a new method for substrate profiling of proteases is presented. the substrates are displayed between two anti-idiotypic affinity domains on the gram-positive bacterium staphylococcus carnosus. the first domain functions as a reporter tag and has affinity for a labeled reporter p ... | 2017 | 27783465 |
| surface display of a single-domain antibody library on gram-positive bacteria. | combinatorial protein engineering for selection of proteins with novel functions, such as enzymes and affinity reagents, is an important tool in biotechnology, drug discovery, and other biochemical fields. bacterial display is an emerging technology for isolation of new affinity proteins from such combinatorial libraries. cells have certain properties that are attractive for directed evolution purposes, in particular the option to use quantitative flow-cytometric cell sorting for selection of bi ... | 2013 | 23064703 |
| in vitro antimicrobial activity screening of xylaria hypoxylon. | fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey. | 2016 | 28852719 |
| antibacterial and bioactive coatings based on rf co-sputtering of silver nanocluster-silica coatings on peek/ bioactive glass layers obtained by electrophoretic deposition. | bioactive and antibacterial coatings on stainless steel substrates were developed and characterized in this study. silver nanocluster-silica composite coatings of 60-150 nm thickness were deposited using radio frequency (rf) co-sputtering on peek/bioactive glass (bg) layers (of 80-90 µm thickness) which had been electrophoretically deposited onto stainless steel. two sputtering conditions were used by varying the deposition time (15 min and 40 min); the resulting microstructure, composition, adh ... | 2017 | 28857546 |
| combination of essential oil compounds and phenolic acids against escherichia coli o157:h7 in vitro and in dry-fermented sausage production. | escherichia coli o157:h7 is a foodborne pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. the low dose of infection and severity of the disease represent a concern to public health. natural compounds have been widely applied as food additives to replace synthetic preservatives. the aim of this study was to determine the efficiency of essential oil compounds (eocs) in combination with phenolic acids (pa) in vitro and in dry-fermented sausage production. minimum inhibitory co ... | 2017 | 28843125 |
| cu-releasing bg/pcl coating on mg with antibacterial and anticorrosive properties for bone tissue engineering. | copper (cu)-containing bioactive glass nanoparticles (cu-bgns) were introduced in polycaprolactone (pcl) coating systems to improve the bioactivity, antibacterial property, and corrosion resistance of vulnerable magnesium matrices under physiological conditions. the influence of different amounts of cu-bgns in pcl coatings was thoroughly investigated determining the wettability, electrochemical properties, and antibacterial effects against staphylococcus carnosus and escherichia coli, as well as ... | 2017 | 28832341 |
| determination of the nano-scaled contact area of staphylococcal cells. | bacterial adhesion is a crucial step during the development of infections as well as the formation of biofilms. hence, fundamental research of bacterial adhesion mechanisms is of utmost importance. so far, less is known about the size of the contact area between bacterial cells and a surface. this gap will be filled by this study using a single-cell force spectroscopy-based method to investigate the contact area between a single bacterial cell of staphylococcus aureus and a solid substrate. the ... | 2017 | 28695218 |
| effect of six different starter cultures on the concentration of residual nitrite in fermented sausages during in vitro human digestion. | the objective of this study was to determine the effect of six different starter cultures of enterobacteria on the concentration of residual nitrite in fermented sausages during in vitro human digestion. before digestion, the concentration of residual nitrite was dependent on starter culture in fermented sausage and ranged from 25.2 to 33.2mg/kg. among the six starter cultures of enterobacteria, pediococcus acidilactici, pediococcus pentosaceus, and staphylococcus carnosus showed higher nitrite ... | 2018 | 28873604 |
| effects of glucose and oxygen on arginine metabolism by coagulase-negative staphylococci. | coagulase-negative staphylococci (cns) are not only part of the desirable microbiota of fermented meat products but also commonly inhabit skin and flesh wounds. their proliferation depends on the versatility to use energy sources and the adaptation to fluctuating environmental parameters. in this study, the conversion of the amino acid arginine by two strains with arginine deiminase (adi) activity (staphylococcus carnosus 833 and s. pasteuri αs3-13) and a strain with nitric oxide synthase (nos) ... | 2017 | 28399999 |
| genetic diversity and population structure of food-borne staphylococcus carnosus strains. | the species staphylococcus carnosus is a non-pathogenic representative of the coagulase negative staphylococci. specific strains are applied as meat starter cultures. the species consists of two subspecies, s. carnosus ssp. carnosus and s. carnosus ssp. utilis. in order to place s. carnosus strains, characterized in former studies, in a genetic background that allows a typing of candidates for starter cultures, a multilocus sequence typing (mlst) scheme was developed. internal fragments of the g ... | 2017 | 27931747 |
| kinetics of volatile marker compounds during ripening of cured loins inoculated with staphylococcus carnosus. | the current study was designed to investigate the influence of ripening time (12 weeks, 15 °c) on the volatile compounds and sensory acceptance for north european cured loins inoculated with a proteolytic staphylococcus carnosus starter culture. | 2017 | 27874964 |
| complete genome sequence of staphylococcus carnosus lth 3730. | specific strains of the apathogenic coagulase-negative species staphylococcus carnosus are frequently used as meat starter cultures, as they contribute to color formation and the production of aroma compounds. here, we report the complete genome sequence of s. carnosus lth 3730, a strain isolated from a fermented fish product. | 2016 | 27688338 |
| use of tocopherol extract and different nitrite sources and starter cultures in the production of organic botifarra catalana, a cooked cured sausage. | this research evaluates the effects of adding a tocopherol mix (0 or 200 mg/kg), two nitrite sources (sodium nitrite or a nitrate-rich vegetable concentrate) and the use of staphylococcus carnosus together with fermentation types that varied in temperature (12 h at 4 ℃ or 16 ℃) on different quality parameters and acceptability of cooked cured sausages after vacuum packing and storage at 4 ℃ for 120 days. in the presence of s. carnosus, residual nitrate and nitrite levels were reduced. sausages c ... | 2016 | 25990636 |
| draft genome sequence of staphylococcus carnosus subsp. utilis lth 7013, isolated from south tyrolean ham. | staphylococcus carnosus is used as a starter culture in meat fermentation, where it contributes to color formation and produces aromatic compounds. here, we report the first draft genome sequence of an s. carnosus subsp. utilis strain, lth 7013, isolated from south tyrolean ham, with potential application as a starter culture. | 2015 | 25977432 |
| secretome analysis revealed adaptive and non-adaptive responses of the staphylococcus carnosus femb mutant. | femabx peptidyl transferases are involved in non-ribosomal pentaglycine interpeptide bridge biosynthesis. here, we characterized the phenotype of a staphylococcus carnosus femb deletion mutant, which was affected in growth and showed pleiotropic effects such as enhanced methicillin sensitivity, lysostaphin resistance, cell clustering, and decreased peptidoglycan cross-linking. however, comparative secretome analysis revealed a most striking difference in the massive secretion or release of prote ... | 2015 | 25430637 |