| clinical aspects of alpha 1-fetoprotein determination in human liver cancer and in humans and experimental animals at carcinogenic risk. | recent findings concerning the significance of alpha 1-fetoprotein (afp) as a tool for clinical diagnosis and monitoring of tumor diseases are reviewed briefly. the applicability of this protein marker to the early diagnosis of patients at carcinogenic risk is discussed. in addition, experimental data obtained with a model of chemical hepatocarcinogenesis are reported. the increase of proliferative activity in precancerous liver tissue preceded afp production under experimental conditions with a ... | 1979 | 89199 |
| antigenic changes associated with liver carcinogenesis. | carcinogen-induced experimental hepatomas are often characterized by new individually distinct antigens capable of inducing tumor immunity in syngeneic hosts. these antigens arise as a consequence of cell-carcinogen interaction and may result from modification or replacement of normal cell-surface components. their role in immunosurveillance is not established, but they offer a target for tumor immunotherapy. reexpressed fetal antigens have also been detected, either as secretory products (alpha ... | 1979 | 89200 |
| antigenic analysis of human heart tissue--characterization of cardiac antigens. | the human heart antigens demonstrated in citric acid extracts by immunodiffusion were submitted to further analysis. heart antigens were precipitated at 30% to 70% saturated ammonium sulphate. these fractions contained antigens reactive with rabbit antisera to human heart. the third fraction out of four proteins which were separated by sephadex g-150 gel filtration, was reactive against rabbit antisera to human heart. the intensely stained proteins of both heart antigens were located at the nine ... | 1979 | 89208 |
| [change in the thresholds of development of ventricular arrhythmias after stimulation of negative emotiogenic hypothalamic centers]. | the dynamics of changes in the thresholds of the origin of ventricular extrasystole, paroxysmal ventricular tachysystole and ventricular fibrillation in long-term stimulation of the ventromedial hypothalamic nucleus were studied in experiments on rabbits. it was found that myocardial resistance to the development of arrhythmias depended on the manifestation of the sympathetico-parasympathic interaction on the heart. in predominance of parasympathetic effects on the heart during hypothalamic stim ... | 1979 | 89222 |
| [simple method of obtaining erythrocytic diagnostic agents for titration of antimeningococcal antibodies]. | | 1979 | 89237 |
| [various tests for detection of extrachromosomal elements of staphylococci]. | | 1979 | 89238 |
| [method of preparing igg, a component of soluble immune complexes of human blood serum]. | | 1979 | 89256 |
| [technic of the ring precipitation test]. | | 1979 | 89259 |
| [preparation of antisera by combined administration of antigens]. | | 1979 | 89268 |
| current concepts of pathogenesis and treatment of myasthenia gravis. | | 1979 | 89275 |
| control of hospital epidemic of gentamicin-resistant klebsiella aerogenes. | | 1979 | 89322 |
| automatic recognition of inter-ictal epileptic activity in prolonged eeg recordings. | a method of automatic recognition and quantification of inter-ictal epileptic activity in the human eeg had previously been developed and tested using short recordings from awake subjects. this paper describes the adaptation of the method for use during overnight recordings in free-moving unattended patients, in combination with the already existing seizure monitoring system. eeg s were recorded from scalp and sphenoidal electrodes, using cable telemetry and a pdp-12 computer. the spike and shar ... | 1979 | 88339 |
| vestibulo-ocular responses during the states of sleep in the cat. | the vestibulo-ocular response (vor) was recorded during natural sleep in cats with chronically implanted electrodes. by using a small amplitude sinusoidal head rotation (11 degrees) peak-to-peak at 0.4 hz) which elicited only slow compensatory eye movements, the vor amplitude was found to decrease steeply (down to 40% or less) during slow-wave sleep. the phase of the vor with respect to head position remained approximately constant. with a larger amplitude of sinusoidal rotation (320 degrees pea ... | 1979 | 88340 |
| [heterogeneity in organization of lipids in the bacterial membrane]. | the lipids of micrococcus lysodeikticus membranes were 50%-substituted by phosphatidyl choline using lipid-exchanging proteins isolated from rat liver. the incorporation of phosphatidyl choline into the membrane did not significantly change the malate dehydrogenase activity and the temperature dependence activity in the arrhenius plots for the enzyme. gramicidin s--modifier of membrane lipids--had similar effects both on the intact membranes and on the phosphatidyl-enriched membranes. a conclusi ... | 1979 | 88966 |
| induction of human antigen-specific and non-specific helper factors in vitro. | human peripheral blood leucocytes, cultured for 4--6 days with the appropriate concentration of protein or synthetic polypeptide antigen, yield helper cells. these cells, after a further 24 hr incubation with the appropriate antigen release helper factors, which are of two types, antigen specific and non-specific; a similar situation to that described in the mouse. these factors are assayed by their effects on the plaque-forming cell response of mouse spleen cells cultures in vitro. the potentia ... | 1979 | 89019 |
| cimetidine: interaction with oral anticoagulants in man. | in 6 patients anticoagulated with warfarin, nicoumalone, or phenindione the addition of cimetidine prolonged the prothrombin-time (pt) by a mean of 12.6 s (range 5--23 s). in 7 volunteers taking daily subtherapeutic doses of warfarin the addition of cimetidine increased the pt from 19.4 to 22.9 s and the plasma-warfarin concentration from 0.96 to 1.76 microgram/ml. cimetidine reduced the single-dose clearance of warfarin and antipyrine. the basis of the interaction between cimetidine and oral an ... | 1979 | 89387 |
| eosinophil counts in duodenal tissue in cow's-milk allergy. | | 1979 | 89421 |
| gentamicin resistance in staphylococcus aureus. | | 1979 | 89440 |
| prospective study of chlamydial infection in neonates. | chlamydia trachomatis was recovered from the cervices of 4% (36/900) of pregnant women tested. 20 infants born through chlamydia-infected cervices were followed up for a year, as were 18 infants born to chlamydia-negative mothers. a statistically significant excess of conjunctivitis and pneumonia was found in infants exposed to chlamydia. the attack-rate for inclusion conjunctivitis was 35% (7/20) and for chlamydial pneumonia it was 20% (4/20). chlamydiae were recovered from 10 of the 20 (50%) e ... | 1979 | 89446 |
| thymidine sensitivity of cultured leukaemic lymphocytes. | cultured leukaemic lymphocytes from patients with t, null, or pre-b acute lymphoblastic leukaemia are shown to be highly sensitive to growth inhibition by thymidine. thymidine sensitivity was correlated with reduced activity of the catabolic enzyme thymidine phosphorylase and sustained elevation of the deoxythymidine triphosphate pool after exposure to thymidine. it is suggested that thymidine may have a role in the management of certain acute lymphoblastic leukaemias of lymphomas. | 1979 | 89451 |
| continuous measurement of ph in central arteries and veins. | | 1979 | 89503 |
| anticonvulsant drugs and cancer. a cohort study in patients with severe epilepsy. | over 2000 epileptic patients admitted to the chalfont centre for epilepsy between 1931 and 1971 and taking anticonvulsants were followed up to the end of 1977. mortality between 1951 and 1977 was greatly in excess of that in the general population of england and wales in that period allowing for age and sex. some of the excess was directly attributable to epilepsy, but there were also more deaths from suicide and circulatory, respiratory, and malignant disease than would be expected. apart from ... | 1979 | 89512 |
| antiviral compound effective against experimental scrapie. | | 1979 | 89602 |
| shelf-life of citric acid-toluidine blue o-so2 and its influence on feulgen staining. | the paper reports on the preparation of a dye-so2 reagent employing a schiff-type dye, toluidine blue o. the method is to replace 5 ml of n hcl per 100 ml of the dye solution by citric acid. the usual potassium metabisulphite is then added. the ph of this new modified dye-so2 reagent is 2.5 as against 1.6 for the hydrochloric acid-toluidine blue o-so2. the shelf-life of this newly developed dye-so2 reagent is four weeks with appreciable reduction of staining intensity after this period as compar ... | 1979 | 89614 |
| simulation and prevention of retrovirus--specific reactions by mycoplasmas. | from human mycosis fungoides tumor-derived cell lines, mycoplasma hyorhinis was isolated. this mycoplasma shared the following characteristics with retroviruses: uptake of 3h-uridine, but not of 3h-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3h-tmp into high molecular weight material in standard reverse transcriptase assays with the template-pri ... | 1979 | 89623 |
| prostaglandin e1 activation of heart camp-dependent protein kinase: apparent dissociation of protein kinase activation from increases in phosphorylase activity and contractile force. | | 1979 | 89626 |
| cea-like antigens in different histologic types of human lung carcinoma. | comparative studies on the presence and immunological reactivity of cea and cea-like antigens in lung and digestive tract cancers were performed. specific goat and rabbit anti-cea immune sera were used. immunofluorescence studies revealed in histologically different types of the lung carcinomas the presence of the cross reacting antigen with classical cea. absorption procedures, and double diffusion tests have shown the existence of two at least antigenic determinants in cea molecule: 1. the det ... | 1979 | 89630 |
| carcinomas of the liver in rats ingesting kepone. | young male and female albino rats ingested 0, 1, 5, 10 or 25 ppm kepone, an organochlorine pesticide, in the diet for two years. carcinomas of the liver, as well as hyperplastic nodules and moderate and severe diffuse hyperplasia were observed in kepone-treated rats. such hepatic lesions were not seen in control rats. female rats ingesting kepone were more susceptible than male rats to hepatic carcinogenesis. rats ingesting 50 or 80 ppm kepone developed severe diffuse hepatic hyperplasia and did ... | 1979 | 89631 |
| [experimental study on intraventricular and intrathecal administration of antibiotics (author's transl)]. | | 1979 | 89640 |
| characterization of murine hepatoma bw7756. i. selected biochemical properties of liver and hepatoma. | selected biochemical properties, based on hepatocellular function, were assessed in the mouse hepatoma bw7756 and host and/or normal mouse liver. these biochemical properties included (a) alpha-fetoprotein (afp) production, (b) lipid composition, (c) isozyme patterns and enzyme activities, and (d) cyclic amp levels. the tumor evidenced an exponential growth phase and vigorous production of afp in the first 3 weeks following transplant. the concentration of afp in the sera of tumor-bearing mice i ... | 1979 | 89649 |
| [reserpine inhibition of the hypotensive and permeability-promoting effect of o-hydroxyethyl derivatives of rutoside]. | | 1978 | 87166 |
| [convulsant properties of methyseleno-2-benzoic acid in rats]. | | 1978 | 87170 |
| regulation of collagenase. therapeutic considerations. | why the cornea ulcerates, in the sense of what goes awry, may be related to the trapping of wound healing in a phase of proteolytic debridement related to a persistent epithelial defect. the initial avascularity of the cornea makes it particularly vulnerable to proteolytic damage. studies on the biochemistry and cell biology of corneal ulceration have indicated that sequential interactions occur which result in the generation of collagenase activity and the development of ulceration. it is likel ... | 1978 | 89726 |
| human b cell function in a polyclonally induced plaque forming cell system. cell triggering and immunoregulation. | the use of a sensitive pfc assay against a specific antigenic determinant (srbc) following polyclonal triggering of human lymphocytes has provided a unique insight into the complex mechanisms of triggering of human b cells. the system is under the strict regulation of a delicate balance between helper and suppressor influences reflecting in certain circumstances the activity of distinct subsets of immunoregulatory cells. lymphoid cells in certain diseases characterized by disordered immunoregula ... | 1979 | 89732 |
| definition of lymphocyte antigens in rats: rt-ly-1, rt-ly-2, and a new mhc-linked antigen system. | | 1979 | 89738 |
| an immunogenetic analysis of the mhc of the rat--agb(rt1). i. serologic data. | | 1979 | 89739 |
| pharmacological models for characterizing new anti-asthmatics. | | 1978 | 89745 |
| a comparison of the antibacterial properties of some udder creams and ointments. | the antibacterial activity of three udder creams and two ointments was assayed using three different methods. seven different bacterial genera were used as test organisms providing a total of 17 strains. only a single strain of corynebacterium was inhibited by all the preparations. certain strains of staphylococci and streptococci were inhibited by four of the preparations. the gram-negative organisms showed greatest resistance to the antibacterial agents tested. the in vitro assays showed only ... | 1979 | 89750 |
| rescue of a thymotropic, leukemogenic c-type virus from cultured, nonproducer-lymphoma cells of strain c57bl/ka mice. | | 1979 | 89753 |
| interactions of retroviruses with chemical carcinogen. i. noncovalent binding of unactivated polycyclic aromatic hydrocarbons. | | 1979 | 89755 |
| [antigenic composition of adenovirus hexons]. | quantitative relations between the group-specific and the type-specific components of the hexons of adenovirus type 2 and 5 were studied by means of fitc-conjugated fab-fragments of antibodies directed against type 2 and type 5 hexons. from the sedimentation constant of the complexes of hexons and fab in the region of excess of fab we conclude that there are at least 20 determinants on the hexon. half of these are type-specific and the others are group-specific. both components of the type 2 hex ... | 1978 | 89772 |
| the development of our present views on the molecular aspects of human blood group specificity. | | 1979 | 89773 |
| histological diagnosis of myocardial injury. comparison of hematoxylin-basic fuchsin-picric acid (hbfp)-stained sections obtained during autopsy with isolated viable rat cardiac myocytes exposed to anoxia. | the light microscopic appearances in hematoxylin-basic fuchsin-picric acid (hbfp)-stained histological sections from cardiac and skeletal muscle tissue were put in relation to the reactions of isolated viable rat cardiac myocytes exposed to anoxia in suspension and their morphology in paraffin-embedded sections. special attention was paid to prenecrotic phases of myocytic injury which were followed, in viable rat cardiac myocytes, by light microscopy, and confirmed with biochemical assays indica ... | 1979 | 89781 |
| capillary permeability to interstitial microinjections of macromolecules and influence of capillary hydrostatic pressure on endothelial ultrastructure. | the transcapillary flux of horseradish peroxidase (hrp, mol. diam. 50--60 a) and ferritin (mol. diam. 110--120 a), microinjected interstitially into the biceps femoris muscle of rats, was analyzed in the electron microscope. from 1 min postinjection hrp was observed to occupy endothelial plasmalemmal vesicles in all positions to be expected if a vesicular transendothelial transport of material occurs in the interstitium-to-lumen direction. permeation of hrp through the intercellular clefts of th ... | 1979 | 89786 |
| ultrastructure of rat renal tubular basement membrane--meshwork structure demonstration by negative staining. | the tubular basement membrane (tbm) (i.e. tubular basal lamina) of rat kidney was shown to be a fine meshwork by electron microscopy after negative staining. strands of the meshwork formed a regular three dimensional lattice work. the pores of the meshwork were polygonal. there were two main pore sizes: one approximately 30 a in diameter, the other 42--60 a. in view of our previous observation that glomerular and alveolar basement membranes were made up fine meshwork, it is quite possible that t ... | 1979 | 89787 |
| transduction of chloramphenicol resistance determinant by a newly isolated bacteriophage in staphylococcus aureus. | | 1979 | 89789 |
| effect of spermine on number of crossing-over events in post-conjugational recombination in escherichia coli k-12. | | 1979 | 89796 |
| immunologic localization of elastin by electron microscopy. | research on the pathogenesis of experimental emphysema has involved studies of the distribution of and destruction of elastin in the alveolar interstitium. the ill-defined organization of elastin in the alveolar interstitium makes it difficult to identify the elastin specifically by staining procedures ordinarily used for electron microscopy. this problem becomes more significant when the elastic tissue is fragmented during emphysema development and localization of the elastin fragments is essen ... | 1979 | 89814 |
| steroid secretion by in vitro perfused testes: inhibitors of testosterone biosynthesis. | | 1979 | 89816 |
| effect of encephlitogenic protein. ppd and tetanus toxoid on leukocyte migration in agarose. a study of "cross-reactivity". | the reactivity to three antigens: bovine encephalitogenic protein (ep), ppd, and tetanus toxoid, was studied with blood leukocytes from healthy humans using clausen's (5) leukocyte migration in agarose technique. there is an obvious correlation between the reaction of ep (all concentrations studied) and to low concentrations of ppd; and between the reactivity to low concentrations of ep and low concentrations of tetanus toxoid. after vaccination with tetanus vaccine, a marked increase in reactiv ... | 1979 | 89821 |
| antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. | | 1979 | 89825 |
| [ultrastructure of mycoplasma sp. demonstrated by phosphotungstic acid-chromic acid ph 0,4]. | ultrastructural observation of mycoplasma sp. revealed by the phosphotungstic acid-cromic acid, ph 0,4. arq. inst. biol., são paulo, 45(3) :169-176, 1978. cells of ib-rs-2 (clone c-26-3) swine line were examined under electron microscopy, employing the differential coloration with pta-acr 5 %, ph 0,4, during 30 minutes, at 37 degrees c. through this method we could observe the intracitoplasmatic forms, electronically dense, rather pleomorfic, with sizes varying between 60nm and 640nm and a unita ... | 1978 | 89840 |
| inhibition of histamine release from rat mast cells by cytochalasin a and other sulfhydryl reagents. | | 1979 | 89848 |
| reinforcing stimulation of the postero-lateral hypothalamus and mating behaviour in the rat. | male rats implanted with chronic electrodes into the postero-lateral hypothalamic site were tested for self-stimulation behaviour. rats exhibiting steady self-stimulation behaviour were observed during mating tests with an oestrus female. during these tests the hypothalamus of male rats was stimulated. results show that no stimulus-bound sexual behaviour was observed. nevertheless, correlations were found between the rewarding value of the cerebral stimulation and the specific sexual components ... | 1978 | 87181 |
| nucleolus organizer regions in two species of bovidae. | silver-staining of nucleolar organizer regions (nors) in somatic cells of holstein cattle (bos taurus) and european buffalo murrah type (bubalus bubalus) indicates that the nors are located in the telomeres of chromosomes 2,3,4,11, and 29 of cattle and chromosomes 3,4,6,23, and 24 of buffalo. chromosome identification was by q-banding. analysis of 326 metaphase cells showed a mean value of 7.7 +/- 1.2 for ag-nors with a range between 4 and 10 for cattle. in all of the cells examined, either one ... | 1979 | 89140 |
| specific precipitation of mouse and rat alpha 2-macroglobulin by immune sera directed against murine leukemia viruses. | leukemia viruses preparations obtained from plasma of leukemic mice or rats were used to immunize rabbits. by immunoelectrophoresis, these non-absorbed antisera reacted specifically with one single mouse serum alpha 2 globulin which was further identified as alpha 2-macroglobulin by several criteria, including gel chromatography and nitracentrifugation. when mouse plasma derived virions were used as antigen, they gave rise to antibodies cross-reacting completely with the corresponding mouse and ... | 1979 | 89873 |
| effects of toyocamycin on the biological activity of a murine oncornavirus produced by a chronically infected cell line. | production of friend virus by chronically infected cells (friend-eveline cell line) is depressed by toyocamycin, an adenosine analogue, at doses which stop cellular growth. the infectivity and the endogenous reverse transcriptase activity of the virions are diminished. these effects are likely to be due to the incorporation of toyocamycin into the 70s rna. | 1979 | 89874 |
| a radioimmunoassay for erythropoietin. | we describe the development of a radioimmunoassay (ria) for erythropoietin. antisera were raised in rabbits with an impure human urinary erythropoietin preparation used as immunogen, but with pure human erytropoietin serving as the labeled antigen in the ria and as a primary standard. the immunoreactivity of erythropoietin is not altered significantly by the mode of labeling with radioiodine, even though the biologic activity is lost. with this method, it is possible to detect 2--3 mu of erythro ... | 1979 | 89877 |
| receptor-mediated binding and internalization of leukocyte elastase by alveolar macrophages in vitro. | radioiodinated leukocyte elastase was found to bind rapidly and specifically to alveolar macrophages in vitro. in contrast to the binding of pancreatic and bacterial proteases, leukocyte elastase binding did not require the presence of alpha 2 macroglobulin. the binding was inhibited by an excess of unlabeled enzyme and was saturable by increasing elastase concentrations. leukocyte elastase binding thus met criteria for receptor-mediated binding, with and estimated association constant of 4.97 x ... | 1979 | 89120 |
| [radiotherapy, bleomycin and immunotherapy (psk, bcg-cws) in esophageal neoplasms]. | | 1979 | 89176 |
| relationship of hepatitis b surface antigen to serum alpha-fetoprotein in nonmalignant diseases of the liver. | serum alpha-fetoprotein (afp) levels were measured by radioimmunoassay in 89 healthy adult chinese, 170 patients with histologically verified non-malignant liver diseases, and 14 hepatitis b surface antigen (hbsag) carriers with normal liver histology. in 97% of the healthy adults, afp levels were under 20 ng/ml, which is then regarded as the normal upper limit. cases with supranormally elevated afp levels ranged from 15-51% in chronic hepatic disorders and were 33% in acute hepatitis. none of t ... | 1979 | 89892 |
| structure of the gene n:gene ns intercistronic junction in the genome of vesicular stomatitis virus. | | 1979 | 89911 |
| coupling between neuronal activity and focal blood flow in experimental seizures. | local blood flow, ecog and single cortical neurone activity were recorded simultaneously from single microelectrodes in 17 cats. seizures were induced by repeated intravenous injections of pentylenetetrazol (ptz, 10-20 mg/kg) or by local application of 1 m na-penicillin. seven to 20 sec after appearance of burst activity in cortical neurones and ecog, focal flow increased up to 300% of control. the extent of this flow increase was significantly correlated with the change in firing rate of the ne ... | 1979 | 89943 |
| a functional measure of brain activity: brain stem transmission time. | surface-recorded auditory nerve and brain stem responses are being used routinely for diagnostic purposes in man. when interest is in auditory diagnosis, the electric response threshold is of primary importance. however, when used in neurological diagnosis, the wave form of the response is important. as a measure of one aspect of response wave form, this paper suggests the use of brain stem transmission time (btt), defined as the time interval between the first earlobe-negative wave (response of ... | 1979 | 89952 |
| pattern of condensation of mouse and chinese hamster chromosomes in g2 and mitosis of 33258-hoechst-treated cells. | | 1979 | 89960 |
| in vitro erythropoiesis. ii. cytochemical enumeration of erythroid stem cells (cfu-e and bfu-e) from normal mouse and human hematopoietic tissues. | a procedure which provides more accuracy in the identification and scoring of in vitro derived erythroid colonies (i.e. bfu-e and cfu-e) from normal mouse and human marrow and spleen cells has been developed. up to now identification of erythroid colonies has been based on staining with acidified benzidine which consistently displays "background staining" of granulocyte/macrophage colonies. the staining reaction with these reagents is such that identification of erythroid colonies is less than p ... | 1979 | 89963 |
| the expression of ia antigenic determinants on macrophages required for the in vitro antibody response. | a subpopulation of antigen-presenting macrophages required for an in vitro antibody response to burro erythrocytes was deleted by pretreating the splenic macrophages with anti-ia serum and complement (c). the in vitro response of the macrophage depleted t-b cell population could not be restored by the addition of macrophages resistant to anti-ia antibodies and c (ia-). the response of ia- macrophages and the macrophage-depleted t-b cells was only reconstituted by the addition of ia+ macrophages. ... | 1979 | 87431 |
| a histological and histochemical study of changes of fiber types in experimental myotonia. | in both fast and slow muscles of rats treated with 20--25 diazacholesterol there were qualitative alterations, such as changes of fiber outlines, numerous moth-eaten fibers and rare ring fibers. in addition there were generally larger groups of type i and intermediate fibers than in normal controls ("type-grouping" tendency) in the preparations for oxidative enzymes in the extensor digitorum longus (edl) of myotonic animals. quantitative evaluations of edl and soleus of myotonic rats revealed mo ... | 1979 | 87497 |
| somatosensory evoked potentials following stimulation of the trigeminal nerve in man. | somatosensory evoked potentials following trigeminal nerve stimulation can regularly be recorded from the contralateral scalp on c5/c6 (10--20 system), a region which overlies the primary face region of the somatosensory cortex. from the first three peaks analyzed (n 13, p 19 and n 26), the first positive peak (p 19) is most prominent and reliable and therefore is recommended for the routine measurements of neurophysiological examination. | 1979 | 87502 |
| the effect of full-thickness skin grafts on the actomyosin content of contracting wounds. | in evaluating the myofibroblast theory of wound contraction, comparisons were made between grafted and nongrafted 15-day wounds. pregnant rabbit uteri and normal rabbit skin were used as controls. small amounts of actin and myosin were present in contracting open wounds but not in noncontracting grafted wounds, which suggests some participation for contractile proteins in wound contraction. the current study refutes the widely accepted contention that wound granulation tissue is as rich as smoot ... | 1979 | 87511 |
| surface antigens of human glioma cells shared with normal adult and fetal brain. | | 1979 | 88260 |
| properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line. | two glycoproteins, gp-1 and gp-2, have been isolated from an extracellular membrane synthesized in cell culture by an embryonal carcinoma-derived cell line. the amino acid and carbohydrate compositions have been determined. both proteins are rich in half-cystine residues and contain approximately 12-15% carbohydrate. antibodies have been obtained against one of the glycoproteins, gp-2, in rabbits. the antibody reacts with basement membranes from adult mouse and human kidney glomeruli and tubules ... | 1979 | 88263 |
| differential arousal response to gustatory stimuli in the awake rabbit. | ecog arousal response as elicited by deionized water and by several concentrations of sweet, salty, sour and bitter tasting substances, all applied intraorally, were studied in the awake restrained rabbit. the study was carried out on 17 chronic preparations of adult male animals (2.6 kg average body weight). water as a stimulant was presented in 60 trials and tastants across qualities and concentrations in 245 trials. arousal was quantitatively characterized by its duration, frequency increment ... | 1979 | 88352 |
| release of prolactin and lh and histamine-containing cells in brain. | the effects of histamine released from mast cells by the compound 48/80, a degranulating agent, on plasma prolactin and lh levels, were examined in rats. compound 48/80 given systemically (s.c.) at a dose of 2 mg/100 g b.w. significantly increased at 15 and 60 min after injection, both prolactin and lh release in ovariectomized, steroid-primed rats. prolactin but not lh was also augmented in the plasma of male rats following s.c. administration of compound 48/80. there were no modifications of p ... | 1979 | 88502 |
| immunospecificity of nuclear nonhistone protein-dna complexes in colon adenocarcinoma. | tumor-specific antisera against dehistonized chromatin isolated from transplantable colon adenocarcinoma (from male noninbred sprague-dawley rats) were produced. the specificities of these antisera were determined by complement fixation. in the presence of these antisera, only chromatin from colon adenocarcinoma significantly fixed complement, whereas chromatins isolated from normal rat colon epithelia were inactive. administration of 1,2-dimethylhydrazine to rats produced an early change in the ... | 1979 | 88536 |
| oncofetal antigen: a tumor-associated fetal antigen immunogenic in man. | oncofetal antigen (ofa) has been defined with the use of human natural antibodies as a membrane antigen of human cancer cells that cross-reacts with human fetal brain tissues. the immunogen that elicits the antibody is unknown. the present study was undertaken to examine the immunogenicity of the ofa found on tumor cells. postoperative melanoma patients were immunized with ofa-positive melanoma cells. anti-ofa reactivities in the immunized sera were titrated by the immune adherence assay with th ... | 1979 | 88539 |
| effect of age on axoplasmic transport of cholinesterase in rat sciatic nerves. | senile muscle atrophy has been attributed to an impaired ability of old nerves to transport trophic factors. to evaluate the effect of age on axoplasmic transport, we measured the accumulation of cholinesterase activity above a ligature around sciatic nerves of young (7--8 months), middle-aged (19--20 months), and old (31--32 months) male rats. protein content and cholinesterase activity per mm of nerve were higher in middle-aged and old than in young nerves. however, accumulation of cholinester ... | 1979 | 88552 |
| [compartmentation of gramicidin 5 in membranes of sensitive bacteria and protein-lipid interactions]. | gramicidin s is sorbed on the isolated membranes of granicidin-sensitive micrococcus lysodeikticus strain. the antibiotic inhibits the membrane malate dehydrogenase within the temperature range of 9--42 degrees c, i.e. under conditions of gel and liquid-crystalline lipid state; however its effect at 10 degrees c is 10 times as low as is observed at 42 degrees c. the inhibitory effect of gramicidin s on malate dehydrogenase can be eliminated and the antibiotic can be removed from the membrane by ... | 1979 | 88965 |
| piroxicam pharmacokinetics in man: aspirin and antacid interaction studies. | pharmacokinetic studies with piroxicam, a nonsteroidal antiinflammatory agent, have been carried out following the administration of single and multiple oral doses. a plasma half-life of approximately 45 hours is observed, permitting the use of single daily doses in therapy. enterohepatic recirculation of drug is suggested by the presence of multiple peaks in plasma concentration curves. piroxicam is highly bound to serum proteins. the absorption and disposition of piroxicam are unaffected by th ... | 1979 | 89124 |
| spatial orientation and distribution of antigens within human glomerular basement membrane. | antibodies to gbm have a linear staining pattern along the gbm by immunofluorescence. in this study, we have examined the distribution of human gbm antigens using goodpasture antibody and heterologous rabbit anti-human gbm antibody. goodpasture sera and goodpasture antibody eluted from diseased kidneys reacted as a single linear component within the gbm. rabbit anti-human gbm was shown to react along distinctly separate zones of the inner and outer aspects of the gbm; the central portion of the ... | 1979 | 89178 |
| antigen-antibody system associated with non-a, non-b hepatitis detected by indirect immunofluorescence. | ten chimpanzees were infected with non-a, non-b hepatitis by inoculation of patient serum or serum from a chimpanzee previously inoculated with patient serum. convalescent serum from one of them reacted, in indirect immunofluorescent tests, with some of the hepatocyte nuclei in sections of autologous liver biopsy specimens and specimens from eight of the other chimpanzees. serum from a convalescent patient reacted in the same way. these positive sera did not react with liver sections from uninfe ... | 1979 | 89332 |
| sequestration of staphylococci at an inaccessible focus. | the persistence of staphylococcal arthritis in a patient treated with nafcillin was attributed to intracellular sequestration of staphylococci. these intraphagocytic organisms were protected against bactericidal activity. the infection was cleared by rifampin--an agent which can penetrate leucocyte membranes. when clinical infections do not respond to seemingly adequate antimicrobial treatment, intracellular persistence of bacteria should be considered. | 1979 | 89334 |
| changing pattern of schistosomiasis in egypt 1935--79. | a village in the nile surveyed for schistosomiasis by j. a. scott in 1935 was surveyed again in 1979. the same number of people as in the 1935 survey were randomly selected for investigation by the same parasitological techniques as those used by scott. the prevalence of schistosoma mansoni infection had increased from 3.2% to 73%, whereas s. haematobium infection, which had been very common in 1935 (74%), had almost disappeared (2.2%). in the local district hospital since 1972 the percentage of ... | 1979 | 89343 |
| gastric cancer in patients who have taken cimetidine. | | 1979 | 89345 |
| scabies, a current pandemic. | scabies, caused by the itch mite and transmitted by skin-to-skin contact, is being seen in pandemic proportion. in the united states this infestation is seen in patients of all ages and socioeconomic levels. scabies is often misdiagnosed, and the classic form occurs less frequently in the current cycle. differential diagnosis includes most pruritic dermatoses. diagnosis should be made with certainity, preferably by identification of the mite, before therapy is begun. modern scabicides are high e ... | 1979 | 89671 |
| [effect of pesticides on tissue structures of the lungs and on the course of experimental tuberculosis]. | | 1979 | 89674 |
| biosynthesis of streptomycin. enzymic oxidation of dihydrostreptomycin (6-phosphate) to streptomycin (6-phosphate) with a particulate fraction of streptomyces griseus. | resting cells and to a greater extent permeabilized cells of streptomyces griseus can oxidize dihydrostreptomycin to streptomycin. the dihydrostreptomycin oxidoreductase activity was localized in the 100,000 x g particulate fraction. sucrose density gradient centrifugation of the particulate suspension gave a band at a density of 1.09 which consisted mainly of membrane vesicles. this fraction had high dihydrostreptomycin oxidoreductase activity. s. griseus protoplasts also contain high oxidoredu ... | 1979 | 89869 |
| effects of hexachlorocyclohexane isomers on concanavalin a 'capping' in bovine lymphocytes. | the effects of alpha, beta, gamma, and delta isomers of hexachlorocyclohexane on concanavalin a 'capping' in bovine lymphocytes were evaluated, gamma and delta hexochlorocyclohexane inhibited 'capping' whereas the alpha and beta isomers were without effect. in addition, gamma-hexachlorocyclohexane has been shown to antagonize the maintenance of preformed 'caps' and cause the rapid dispersal of the concanavalin a-receptor complexes over the surface of cells by a temperature-dependent mechanism. t ... | 1979 | 89870 |
| influence of cysteamine on differential staining of budr-substituted human chromosomes. | in 5-bromodeoxyuridine (budr)-substituted human chromsomes stained with 4'-6-diamidino-2-phenylindole (dapi) differential staining is suppressed totally by the h+-donor cysteamine (concentration 0.08 m). we propose that differential staining appears because the double budr-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. it is suggested that cysteamine prevents the production of strand breaks in dna and, consequently, differential staining in budr-substi ... | 1979 | 89890 |
| experimental studies of the pathogenesis of infections due to pseudomonas aeruginosa: extracellular protease and elastase as in vivo virulence factors. | the effects on mortality of supplemental injections of protease and elastase were determined in burned mice infected with non-lethal inocula of a toxin-producing but non-proteolytic-enzyme-producing strain of pseudomonas aeruginosa. when a variety of solutions containing proteolytic enzyme were injected under these conditions, the mortality increased significantly. this did not occur when organisms other than p. aeruginosa were used. injections of the enzyme solutions alone were non-lethal. inje ... | 1979 | 89891 |
| retrograde axonal transport of horseradish peroxidase in regenerating sciatic nerve of the rat. | | 1979 | 89965 |
| an alkaline protease in the delayed hypersensitivity skin lesions in the guinea-pigs. | an alkaline hemoglobinolytic protease was extracted from the delayed hypersensitivity skin lesions induced by bovine gamma-globulin as an antigen in the guinea-pig. the enzyme was heat-labile and inhibited by thiol-blocking reagents. the mol.wt. was more than 100,000 and optimal ph around 9. | 1979 | 89970 |
| vasoactive intestinal peptide (vip) stimulates prolactin (prl) release and camp production in a rat pituitary cell line (gh3/b6). additive effects of vip and trh on prl release. | | 1979 | 89971 |
| [phospholipids and blood coagulation]. | after a simplified survey of the chemistry of phospholipids (pl) their presence in the lipid part of thromboplastin is dealt with, the significance of this structure being stressed. in addition, the condition, mode of action and synthesis of pl in blood platelets are extensively discussed and the impact of the composition of thrombocytic pl in the membrane and granular fraction by plasma lipid substances is referred to. the relations between single forms of pl and those proteins activating coagu ... | 1979 | 89981 |
| human leukaemia-associated antigens expressed by acute lymphocytic leukaemias and their detection with heterologous antisera to t, b-, and non-t-non-b subtype al blasts. | antisera from rabbits and goats against subtypes of acute lymphocytic leukaemia (all with t-cell markers, all with b-cell markers, non-t-non-b all) were tested for their specificity in complement-dependent in-vitro cytotoxicity testing. after absorption of the fivefold diluted antisera with erythrocytes and spleen cells of allogenous donors they reacted with all cells, but not with leukaemias of other types (aml, cll, cml), lymphocytes of healthy donors, enriched b-lymphocytes, enriched t-lympho ... | 1979 | 89983 |
| [the binding of dyes of fibrinogen]. | examinations performed by means of the agargel-electrophoresis, immunoelectrophoresis, discelectrophoresis, and chromatography on gel in human plasma and preparations of fibrinogen revealed that fibrinogen will form complexes with 18 examined dyes of different chemical structure, from a total amounting to 46. the findings indicate a marked affinity of fibrinogen towards small molecules in general. in nearly all complex forming colour materials, fibrinogen could be identified by immunodiffusion i ... | 1979 | 89992 |
| studies on the action of histamine release by persulphates. | | 1979 | 89996 |
| isolation and characterization of diploid clones from adult and newborn rat liver cell lines. | a high frequency of diploid and near-diploid clones were developed from cell lines derived from adult and newborn rat liver using micropipettes. there were some differences in morphology, biochemical properties and growth rate between clones. cloned cells had low levels of tyrosine transaminase activity, glucose-6-phosphatase activity and albumin content. a diploid clone and pseudodiploid clone derived from adult rat liver cell line were positive for alpha-fetoprotein. | 1979 | 90011 |
| revertants of adenovirus type-12-transformed hamster cells have lost part of the viral genomes. | | 1979 | 90022 |
| ocular findings in systemic cutaneous basophil hypersensitivity. | scbh was induced by immunizing guinea pigs with a protein antigen and challenging 1 week later with a large intraperitoneal dose of the same antigen. animals developed a delayed-onset, erythematous skin rash and dermal infiltration by basophils and eosinophils. the uveal tracts of these animals were infiltrated by eosinophils, as were several other internal organs. the eye is affected in scbh, and as in other forms of ocular cell-mediated hypersensitivity, the eosinophil is a prominent cellular ... | 1979 | 90029 |