| [small-angle x-ray scattering determination of the distance between heavy atom labels in hemoglobin and histidine decarboxylase in solution]. | | 1979 | 477512 |
| [hmg-protein of chromatin located in segments sensitive to the action of micrococcal nuclease]. | | 1979 | 477498 |
| on the mechanism of oxygen protection against ionizing radiation damage in the cell wall of micrococcus radiodurans. | | 1979 | 472126 |
| on the secondary and tertiary structure of murein. low and medium-angle x-ray evidence against chitin-based conformations of bacterial peptidoglycan. | | 1979 | 456346 |
| biodegradation of the phthalates and their esters by bacteria. | recent studies on the biodegradation phthalate esters in natural ecosystems, sewage, and laboratory cultures are reviewed. there is ample evidence to demonstrate that bacteria are major elements in the biodegradative processes and that in most situations complete oxidation of the aromatic ring occurs; much less is known about the catabolism of the alcoholic moiety, e.g., 2-ethylhexanol. evidence is presented to support catabolic pathways in pseudomonads and micrococci that are initiated by succe ... | 1976 | 829485 |
| a study of dna denaturation in the ultracentrifuge. | | 1976 | 822895 |
| light-stimulated synthesis of chloroplast dna. | light-stimulated chloroplast dna synthesis was studied during chloroplast development in the absence of cell division and nuclear dna synthesis. incorporation of 32pi was stimulated 10-15 fold, however, the ratio of chloroplast dna to nuclear dna remained constant. isotope dilution experiments suggested that stimulated labeling of chloroplast dna represented more efficient utilization of exogenously supplied pi rather than stimulated turnover of chloroplast dna. the low level of dna synthesis an ... | 1976 | 816375 |
| studies on dna repair in bacillus subtilis. i. a cellular factor acting on gamma-irradiated dna and promoting its priming activity for dna polymerase i. | initiation of new dna synthesis was observed in b. subtilis cells upon gamma-ray irradiation followed by toluene treatment and incubation in the presence of the four deoxynucleotide triphosphates and mg2+. this dna synthesis took place in the absence of atp and was refractory to 6-(p-hydroxyphenylazo)-uracil which is a specific inhibitor for the type iii polymerase of bacillus subtilis. this repair-type dna synthesis was greatly reduced in mutant cells deficient in dna polymerase i. restoration ... | 1975 | 807254 |
| solanesyl pyrophosphate synthetase from micrococcus lysodeikticus. | solanesyl pyrophosphate synthetase from extracts of micrococcus lysodeikticus was purified by deae-sephadex, hydroxylapatite, and sephadex g-100 chromatography. this enzyme was found to catalyze the trans condensation of isopentenyl pyrophosphate with geranyl pyrophosphate to afford all-trans-octaprenyl (c40) and alltrans-nonaprenyl (c45) pyrophosphate without accumulation of prenyl pyrophosphate with chain length shorter than c40. all-trans-farnesyl and all-trans-geranylgeranyl pyrophosphate al ... | 1977 | 911777 |
| [determination of the molecular weight and form of the molecule histidine decarboxylase by the method of small-angle x-ray scattering]. | shape and molecular weight of histidine-decarboxylase from micrococus sp. n. were studied by the method of x-ray small-angle scattering. the inertion radius of the molecule: rg-2,93 nm. the shape of the molecule is adequately approximated by rotation ellipsoids of two possible variants: the elongated and flattened ones. the eccentricity in both cases is 1.6. the volume of the enzyme molecule v=190 nm3. the molecular weight of histidine-dexarboxilase obtained from the x-ray experiment m=102 000 c ... | 1977 | 911898 |
| [study of molecular organization of biological membranes using lipid transfer proteins. asymmetry of phospholipids in the cytoplasmic membrane of micrococcus lysodeikticus]. | | 1977 | 911945 |
| protection by antioxidants against the toxicity of ozone to microbial systems. | | 1977 | 913375 |
| the immune response of micr to micrococcus lysodeikticus: evidence for serum-mediated immunoregulation [proceedings]. | | 1977 | 913812 |
| biochemical properties that accompany the production of homogeneous antibody response: a general mechanism hypothesis [proceedings]. | | 1977 | 913813 |
| primary structure of a high potential iron sulfur protein from a moderately halophilic denitrifying coccus. | the occurrence of a specific high potential iron-sulfur protein in a halophilic, denitrifying coccus provisionally assigned to the genus paracoccus has been confirmed through primary structure determination. this protein, known as "hipip", has been found previously only in photosynthetic bacteria. the sequence of this 71-residue protein is as similar to the hipeps from photosynthetic bacteria as they, in turn, are to one another (average number of identically matching residues is 38%). paracoccu ... | 1977 | 914842 |
| desaturation of fatty acids by the psychrophilic bacterium micrococcus cryophilus [proceedings]. | | 1977 | 923967 |
| amidase activity involved in peptidoglycan biosynthesis in membranes of micrococcus luteus (sodonensis). | membrane suspensions prepared from micrococcus luteus (sodonensis) in both the exponential and stationary phases of growth contained a transglycosidase activity capable of synthesizing linear peptidoglycan. exponential-phase membranes also contained an n-acetylmuramyl-l-alanine amidase activity which degraded the peptidoglycan as it was formed. the product of this amidase was purified and found to be free pentapeptide. the amidase was specific for peptidoglycan and could not attack lower-molecul ... | 1976 | 931948 |
| catabolism of l-tyrosine by the homoprotocatechuate pathway in gram-positive bacteria. | a metabolic pathway for l-tyrosine catabolism involves 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) as substrate for fission of the benzene nucleus. cell extracts of an organism tentatively identified as a micrococcus possessed the enzymes required for degrading homoprotocatechuate to succinate and pyruvate, and stoichiometry was established for several of these reactions. when the required coenzymes were added, cell extracts degraded l-tyrosine to the ring-fission product of homopro ... | 1976 | 931949 |
| the rate of recombination repair and its relationship to the radiation-induced delay in dna synthesis in micrococcus radiodurans. | the measurement of the time at which normal colony-forming ability returns in irradiated cultures of micrococcus radiodurans tsi held at 30 degrees c can be used to estimate the time of completion of recombination repair. by comparing the times to complete such repair in populations given increasing radiation doses it is possible to calculate the rate of recombination repair. the rate was independent of the radiation dose; recombination could repair in one minute the damage caused either by 1-2 ... | 1976 | 932678 |
| differentiation of micrococcus luteus and micrococcus varians on the basis of catalase isoenzymes. | crude extracts prepared from four micrococcus varians strains, 11 m. luteus strains and four laboratory isolates subsequently classified with m. luteus were assayed for catalase activity following electrophoresis on polyacrylamide gels. the enzyme patterns produced from the m. varians strains exhibited three catalase isoenzymes which were distinguished into two types of patterns depending upon the location of the major band. the extracts from all the m. luteus strains produced the same pattern, ... | 1976 | 932680 |
| the inhibition of lysozyme by bile acids. | the effect of bile acids on the bacteriolytic activity of lysozyme towards micrococcus lysodeikticus was studied in vitro. all bile acids tested inhibited lysozyme activity. conjugated bile acids were better inhibitors than their unconjugated homologs and sulfation resulted in still stronger inhibition. a study of uv-difference spectra of bile acid-lysozyme mixtures suggests that bile acids distort the tertiary structure of the enzyme. the inhibition-concentration curves of micelle-forming bile ... | 1976 | 941895 |
| submicrogram quantitation of an acidic polysaccharide by rocket immunoelectrophoresis and rocket affinoelectrophoresis. | | 1976 | 942097 |
| effect of starter culture on staphylococcal enterotoxin and thermonuclease production in dry sausage. | different amounts of enterotoxin a-, b-, and c1-producing staphylococci were added to dry sausage prepared by normal processes, either alone or in conjunction with a starter culture (micrococci and lactobacilli). the sausage was examined after 0, 3, 7, 14, and 30 days for staphylococci, micrococci, and lactobacilli, and measurements were made of water activity, ph, enterotoxin, and thermostable nuclease. the results showed that in the absence of starter culture measurable amounts of enterotoxin ... | 1976 | 942200 |
| preliminary studies on the characterization and distribution of staphylococcus and micrococcus species on animal skin. | a total of 221 strains of staphylococci and 98 strains of micrococci isolated from the skins of eastern gray squirrels, southern flying squirrels, raccoons, opossums, squirrel monkeys, swine, sheep, horses, cattle, and dogs were characterized in a preliminary attempt to resolve their natural relationships and distribution in nature. staphylococci demonstrating the widest host range included staphylococcus xylosus and unnamed staphylococcus sp. 3. unnamed staphylococcus sp. 2 was isolated only fr ... | 1976 | 942208 |
| polyribonucleotides containing thiopurines: synthesis and properties. | | 1976 | 942425 |
| subunit structure of physarum polycephalum chromatin. | | 1976 | 945190 |
| the specificity requirements of bacteriophage t4 lysozyme. | a series of bacterial cell wall glycopeptides of low molecular weight and cell wall nucleotide precursors have been tested for their inhibitory action on the digestion by t4 lysozyme of a radioactively labeled linear uncrosslinked peptidoglycan. the disaccharide-peptides glcnac-murnac-l-ala-d-glu(a2pm) (c5) and glcnac-murnac-l-ala-d-glu(a2pm-d-ala) (c6) as well as the monosaccharide-peptide murnac-l-ala-d-glu(a2pm) were found to be good competitive inhibitors (with similar ki values) whereas th ... | 1976 | 947753 |
| amidase activity of some bacteria. | the amidase activity of bacteria possessing a high nitrilase activity was found to display the same spectrum although the bacteria may belong to different taxonomic groups, bacillus, bacteridium, micrococcus, brevibacteriun. the spectrum of amidase activity, although very broad, is more restricted than that of nitrilase activity. internal amides as well as vinyl-bound amides are not hydrolyzed. | 1976 | 947836 |
| [study of biological membrane structure using phospholipid-transporting proteins]. | | 1976 | 949931 |
| effect of 5'-terminal structure and base composition on polyribonucleotide binding to ribosomes. | | 1976 | 950671 |
| [synthesis of b group vitamins by micrococcus freudenrichii k-219 depending on the growth rate on a carbohydrate medium]. | | 1976 | 950923 |
| synthesis of the monomer and dimer peptide fragments of the micrococcus lysodeikticus cell wall peptidoglycan. | three branched peptides, namely pentapeptide a and the two decapeptides b and c corresponding respectively to the monomer and the two dimer peptide fragments of the m. lysodeikticus cell wall peptidoglycan, were synthetized by adequate methods of peptide synthesis in homogeneous phase. the synthetized peptides showed the same electrophoretic and chromatographic behavior as the natural peptide fragments. only decapeptide b was hydrolysed by the myxobacter al-1 protease by cleavage of the d-ala-l- ... | 1976 | 953049 |
| enzymatic properties of a new type of lysozyme isolated from asterias rubens : comparison with the nephthys hombergii (annelid) and hen lysozymes. | the enzymatic properties of asterias rubens and nephthys hombergii lysozymes were compared with those of hen egg-white lysozyme. the results allowed to conclude that the asterias rubens lysozyme was representative of a new lysozyme type. the nephthys hombergii lysozyme however could be classified among the hen type enzymes with a tendency to exhibit under certain conditions some properties attributed to the goose type lysozymes. | 1976 | 953059 |
| lipid transfer proteins as a tool in the study of membrane structure. inside-outside distribution of the phospholipids in the protoplasmic membrane of micrococcus lysodeikticus. | | 1976 | 962949 |
| significance of dimers to the size of newly synthesized dna in uv-irradiated chinese hamster ovary cells. | dna synthesized after uv irradiation is smaller than that in unirradiated cells even when pulse-labeling times are increased to compensate for the overall reduction in the rate of dna replication. by isolating newly replicated dna, incubating it with dimer-specific endonuclease from micrococcus luteus, and analyzing it on alkaline sucrose gradients, we have been able to demonstrate that this dna is synthesized in segments corresponding in size to the interdimer distance on the parental strand. i ... | 1976 | 963196 |
| improved microbiological procedure for determining bacitracin in premixes and mixed feeds. | an improved microbiological procedure is presented for determining bacitracin in premixes and mixed feeds. premixes are extracted with acidic 50% aqueous methanol, and mixed feeds are extracted with 50% dimethylformamide after washing with acetone to remove fats and pigments. extracts are diluted with ph 6.0 phosphate buffer and assayed by using a 2-point assay system with micrococcus flavus as the test organism. the simpler extraction coupled with the 2-point assay system measured bacitracin le ... | 1976 | 965335 |
| chemical and nutritional evaluation of yeasts and bacteria as dietary protein sources for rats and pigs. | | 1976 | 966729 |
| [on the hazards of nosocomial infections in dental treatment areas (author's transl)]. | using a standardized method of obtaining bacterial counts of surfaces by superficial application and subsequent incubation of agar lamina, density and kind of bacteria present were examined in treatment areas of both individual dentists praxis and of dental poli-clinics. the results obtained showed, that 1. typical bacteria of hospital infections (micrococcus aureus, klebsiella) were present in only small numbers in both individual dental praxis or in clinical dental treatment areas. 2. the medi ... | 1976 | 970021 |
| lysis and killing of bacteria by lysosomal proteinases. | the bacteriolytic and bactericidal effects of the human proteinases cathepsin b, cathepsin d, cathepsin g, and elastase were investigated. cathepsin g and elastase were 5 to 10% as active as egg white lysozyme in the lysis of micrococcus lysodeikticus. all four enzymes slowly lysed the lysozyme-resistant staphylococcus aureus. the gram-negative acinetobacter 199a was rendered sensitive to lysozyme by all of the proteinases. only elastase caused marked proteolysis of the outer membrane, which wou ... | 1976 | 971964 |
| [localization of pyruvic acid in the histidine decarboxylase of micrococcus sp. n]. | a fragment containing the pyruvic acid residue is isolated from hymotryptic hydrolyzate of alpha-polypeptide chain of carboxymethylated histidine decarboxylase (hd) 14c-phenylhydrasone. the pyruvate residue is estimated to be bind with alpha-amino group of n-terminal phenylalanine in alpha-polypeptide chain. n-terminal alanine is found in hd alpha-polypeptide chain after its reductive amination. it makes possible to determine the estimation of n-terminal amino acid sequence in hd alpha-chain. | 1976 | 974173 |
| [isolation of cytochrome b556 from the membranes of micrococcus lysodeikticus bacteria]. | proteins and cytochrome b556 were solubilized from micrococcus lysodeikticus membranes using triton x-100 treatment. passing of this preparation through deae cellulose column in the presence of triton x-100 made possible to isolate cytochrome b556 from other membrane cytochromes and to purify it up to the content of 2.3 nmol per mg of protein. the prostetic group of cytochrome b556 is determined to be protoheme for the spectrum of alkaline pyridinehemochrome. | 1976 | 974177 |
| effect of magnesium and some nutrients on the growth and nuclease formation of a moderate halophile, micrococcus varians var. halophilus. | production of halophilic nuclease by a moderate halophile, micrococcus varians, atcc 21971, was maximal at 2.5 to 3.5 m nacl concentration in a complex medium (cm) composed of 1% casamino acids, 1% yeast extract, and nacl. the addition of 81 mm mgso4 to cm inhibited nuclease production in spite of good growth. microscopic observation showed that this inhibition was accompanied by complete clumping of the cells. the sehgal and gibbons complex medium (sgc) which contained 0.75% vitamin-free casami ... | 1976 | 974905 |
| [chemistry of denitrification in sporogenous soil bacteria]. | soil sporeforming denitrifying bacteria, bacillus filaris and bacillus polymyxa, differ by their cultural-morphological and physiological characteristics, but are similar in the chemistry of dissimilating nitrate reduction. two processes occur simultaneously: denitrification yielding gaseous nitrogen forms, and nitrate respiration upon which nitrates are reduced to ammonia. the ratio between the two depends on physico-chemical conditions of the environment. the chemistry of dissimilating nitrate ... | 1976 | 979677 |
| a study of skin diseases in dogs and cats. ii. microflora of the normal skin of dogs and cats. | a study of the microflora of the normal skin was undertaken in 10 dogs and 10 cats. swabs were taken from the surface of the skin at 7 different sites (fig. 1). micrococcus spp., alpha-hemolytic streptococci, and acinetobacter spp. were found consistently in both species. staphylococcus aureus was isolated from 9 dogs and 4 cats, and staphylococcus epidermidis from 7 dogs and 5 cats. beta-hemolytic streptococci, corynebacterium spp., bacillus spp., escherichia coli, proteus mirabilis, pseudomona ... | 1976 | 980697 |
| bacteria within ovules and seeds. | surface-sterilized ovules and seeds of 27 species of plants were cultured in the water of syneresis of a nutrient medium low in agar content. bacteria were obtained from 30% of the ovules, 15% of the seeds of herbaceous plants, 16% of the seeds of woody plants, 5.4% of the overwintered noncereal seeds, and 13.5% of overwintered cereal seeds. in no instance did every ovule or seed of a plant species contain bacteria. no bacteria were obtained from the hard, waxy seeds of mimosa or yellowwood. the ... | 1976 | 984839 |
| histone redistribution and conformational effect on chromatin induced by formaldehyde. | histone redistributions between endogenous dna in calf thymus chromatin and exogenous dna from clostridium perfringens (69% a + t) or from micrococcus luteus (30% a + t) induced by 0.6 m nacl or by 2% formaldehyde were studied by thermal denaturation. the observed redistribution occurred on histone hl when the exogenous dna was (a + t)-richer than the dna in chromatin, and when the mixture was exposed to 0.6 m nacl or formaldehyde. when a (g + c)-richer dna was added as the acceptor for histones ... | 1976 | 987800 |
| hypersensitivity to bacteria in eczema. iv. cytotoxic effect of antibacterial antibody on skin cells acquiring bacterial antigens. | the sera of persons with generalized eczema (whitfield-type) or with disseminated nummular eczema were examined for complement-activating antibacterial antibodies to test the hypothesis that some eczematous change results from an antibody-mediated cytotoxic reaction. bacteria dying in the stratum corneum release soluble antigens, some of which diffuse into the stratum malpighii and become firmly adsorbed to the epidermal cells. antibacterial antibody and complement diffusing into the epidermis r ... | 1976 | 990168 |
| substrate specificity of the ultraviolet-endonuclease from micrococcus luteus. endonucleolytic cleavage of depurinated dna. | the ultraviolet-endonuclease isolated from micrococcul luteus, specific for pyrimidine dimers, is able to attack not only ultraviolet-irradiated dna (leading to 3'oh-5'po4 single-strand breaks) but also superhelical covalently-closed circular dna of phage lambda damaged by heating at 70 degrees c, ph 5.93. the number of endonuclease-sensitive defects in the dna corresponds to the number of alkalilabile bonds (apurinic sites) induced by heating. competition between ultraviolet-induced lesions and ... | 1976 | 991858 |
| antitumour immunoprotection by an immunobacterial lectin approach. | | 1976 | 991898 |
| amikacin in treatment of infections caused by gram-negative bacteria resistant to gentamicin and other aminoglycosides: clinical and bacteriologic results. | amikacin (250 or 500 mg) was administered intramuscularly twice daily at 12-hr intervals to 34 patients with infections due to various gram-negative bacteria. usually one or more aggravating factors were present, such as serious underlying pathology or therapy with steroids or immunosuppressants. clinical isolates from most patients were resistant to gentamicin and other aminoglycosides. the overall response to therapy was excellent in 20 patients; in eight patients clinical response was good, b ... | 1976 | 993623 |
| actinorhodin is a chromosomally-determined antibiotic in streptomyces coelicolar a3(2). | streptomyces coelicolar a3(2) synthesizes a second antibiotic, in addition to the plasmid-determined methylenomycin a. it was identified, primarily on the evidence of mass spectroscopy of its diethyl ester, as actinorhodin, which has been described previously in other strains. it inhibited most gram-positive bacteria tested, but only at a comparatively high concentration. five independent mutations leading to lack of actinorhodin synthesis were located between cysd and stra on the chromosome. | 1976 | 993778 |
| interaction of ethidium bromide with dna: effect of licl and ethylene glycol. | the interaction of the intercalating dye ethidium bromide with several native and synthetic polydeoxyribonucleic acids has been studied by means of circular dichroic spectra. the cd of dna-ethidium bromide complexes in the 290-360 nm region is characterized, especially at high salt and at high ethylene glycol content, by positive and negative bands near 308 nm and 295 nm, respectively. these dye associated cd bands are unaffected by the addition of licl or ethylene glycol, suggesting that the re ... | 1976 | 995636 |
| lysozyme in hen blood serum. | hen blood serum (white leghorn) possessed the lytic action against micrococcus lysodeikticus which was less than one-thousandth of egg white obtained from hens of the same species, suggesting that lysozyme was present. the filter-sterilized hen blood serum also inhibited the growth of m. lysodeikticus in broth culture. the isolated lysozyme, purified by column chromatography and gel filtration, proved to be a basic protein with a low molecular weight (about 15,000), active against m. lysodeiktic ... | 1976 | 995802 |
| androgen-induced gene activation in the rat prostate. | | 1976 | 999713 |
| interaction of a fluorescent reagent, fluorescein mercuric acetate, with nucleic acids. | fluorescein mercuric acetate (fluorescein hg ac), which is a fluorescent thiol reagent, was shown to bind to various nucleic acids by measuring the changes in its absorption and fluorescence properties. up to a critical concentration of free fluorescein hg ac (1-10(-7) m for calf thymus dna, with 42% gc, and 2-10(-7) m for micrococcus lysodeikticus dna, with 72% gc) this reagent appears to bind selectively to single-stranded sections in dna. above this critical concentration, cooperative binding ... | 1976 | 999906 |
| quantitative determination of n-acetylglucosamine residues at the non-reducing ends of peptidoglycan chains by enzymic attachment of [14c]-d-galactose. | the ability of human milk galactosyltransferase to attach d-galactose residues quantitatively to the c-4 of n-acetylglucosamine moieties at the ends of oligosaccharides has been utilized for the specific labeling and quantitative determination of the chain length of the glycan moiety of the bacterial cell wall. the average polysaccharide chain length of the soluble, uncrosslinked peptidoglycan secreted by micrococcus luteus cells on incubation with penicillin g was studied with this technique an ... | 1976 | 1009945 |
| use of the lysozyme test in the diagnosis of kerato-conjuctivitis sicca in dogs and cats. | comparative studies of the lysozyme test (lt) and the schirmer tear test (stt) revealed that the lt was not reliable in the diagnosis of kerato-conjunctivitis sicca (kcs) in dogs and cats. this is in contrast to its successful use in human patients. no m. lysodeikticus lysating substrates were detectable in the tear fluid of the normal cat. | 1976 | 1013972 |
| an improved bacteriological method for the detection of sulfonamide residues in food. | | 1976 | 1015478 |
| [improved turbidity technic for the determination of lysozyme activity]. | | 1976 | 1016443 |
| activity correlations between similarly modified soluble and immobilized enzymes. | | 1976 | 1021691 |
| [specific modification of free lysine amino groups of histidine decarboxylase from micrococcus sp. n. by trinitrobenzene sulfonic acid]. | it has been found that 14 lysine residues are accessible for trinitrobenzene sulfonic acid (tnbs) in the molecule of histidine decarboxylase (hdc). the other 62 lysine residues in the molecule of native hdc are masked and inaccessible for tnbs. it is demonstrated that the sh- and alpha-amino groups of methionine are not modified by tnbs. a correlation between the decarboxylase activity of the enzyme and the degree of its trinitrophenylation has been studied. hdc, whose molecule contains 3--9 tnp ... | 1976 | 1022283 |
| [amino acid sequence in tryptic peptides of maleylated micrococcus sp. n. histidine decarboxylase beta-polypeptide chain]. | maleilated histidine decarboxylase beta-polypeptide chain, containing 3 arginine residue, was hydrolysed by trypsin. 4 non-overlapping homogenous peptides were isolated, 3 of them containing one arginine residue and the 4th peptide being c-terminal fragment of beta-chain. beta-polypeptide chain is found to consist of 78 amino acid residues and to have molecular weight of 8456. primary structure of each peptide and their possible sequence in beta-chain are determined. | 1976 | 1024578 |
| appearance of morphologically aberrant cells of micrococcus lysodeikticus (luteus) in medium with papain. | | 1976 | 1026231 |
| [application of phase partition method for the fractionation of hydrophobic membrane proteins]. | phase partition method in a two-phase polyethylene glycol-dextrane system has been applied to fractionation in triton x-100 of hydrophobic membrane proteins from micrococcus lysodeikticus. this method allowed to separate the cytochrome b556 from other cytochromes. spectral and gel electrophoretic patterns of isolated cytochrome are given. | 1976 | 1030632 |
| [proteins of bacterial membranes. purification of nadh-dehydrogenase by electrofocusing]. | a highly purified preparation of nadh dehydrogenase was isolated from bacteria m. lysodeikticus membranes. the purification procedure involved extraction of the enzyme complex from isolated membranes by edta, solubilization of the complex by non-ionogenic detergent (1% triton x-100), chromatography on deae-cellulose and electrofocussing in the ph gradient 4-6. the isoelectric point of the preparation is at 4.5; its main component is a protein with m.w. of about 76.000. | 1976 | 1030633 |
| airborn bacteria in the atmosphere of kuwait. | | 1976 | 1037051 |
| hypersensitivity to bacteria in eczema. ii. titre and immunoglobulin class of antibodies to staphylococci and micrococci. | the amounts of agglutinating antibody to staphylococcal and micrococcal phenol-extracted (probably teichoic acids) and protein antigens, prepared from baird parker types s1, siv and m1, were no greater in eczematous than in control persons. most antibody to phenol-extracted to phenol-extracted antigens was igm which frequently activated complement to lyse red cells which had adsorbed the bacterial antigen. igg antibody against phenol-extracted staphylococcal antigen (staph. aureus, baird parker ... | 1976 | 974019 |
| polyribonucleotides containing thiopurines: synthesis and properties of poly (6-thioguanylic acid). | the synthesis of poly(2-amino-6-chloropurinylic acid) [poly(n2cl6pu)] by the polynucleotide phosphorylase catalyzed polymerization of 2-amino-6-chloro-9-(beta-d-ribofuranosyl)purine 5'-diphosphate and its chemical conversion to poly(6-thioguanylic acid) [poly(s6g)] is described. poly(s6g) was found to form a relatively unstable complex with poly(c), the properties of which were incompatible with those previously reported for the same complex prepared by another method [darlix, j.l., fromageot, p ... | 1976 | 974065 |
| binding isotherms and the interaction between proflavin and a dna of high g-c content. | binding isotherms corresponding to several situations of ligand binding to a linear polymer are calculated, including cases of cooperativity or anticooperativity between the bound ligand states, multiple binding modes that are competitive or non competitive, and possible exclusive of an arbitrary number of adjacent sites upon occupancy of a site by a single ligand. the sequence generating function method of lifson and bradley is used, requiring the assumption that no end effects are involved. th ... | 1976 | 974225 |
| [antibiotic resistance of staphylococcus epidermidis and micrococci isolated from diagnostic materials in hospitals in gdańsk]. | | 1976 | 980944 |
| nomenclature of staphylococci and micrococci. | | 1976 | 984872 |
| thymineless death in a thymine-dependent mutant of micrococcus radiodurans t2 in the presence of chloramphenicol and rifampicin. | a possibility to prevent cells of a thymine-dependent mutant of micrococcus radiodurans t2 from thymineless death was investigated. it was found that the presence of chloramphenicol (cap) in a thymineless medium only decelerated the death of cells. the presence of rifampicin (rfp) considerably decreased the death rate of cells but could not prevent thymineless death completely. | 1976 | 992504 |
| selective medium for distinguishing micrococci from staphylococci in the clinical laboratory. | a nitrofuran-containing medium, fto agar, supported the growth of micrococcus and prevented the growth of staphylococcus. its potential as a differential medium is considered worthy of clinical trial. | 1976 | 993328 |
| effect of agarose variability on the measurement of lysozyme activity. | lysozyme assays are often performed by a diffusion technique utilizing agarose gels impregnated with substrate organisms (lysoplates), but the results differ greatly from those obtained with spectrophotometric or immunologic techniques. we have investigated the effect of agarose composition on the lysoplate assay utilizing 10 different gels varying in ionic parameters. standard curves generated with purified human lysozyme solutions were parallel, but the diameters of the zones of lysis varied i ... | 1976 | 1000849 |
| oxytetracycline residues in meat and kidney tissue after intramuscular or intramammary treatment as determined by a chemical-physical method and compared to a microbiological method. | two adult cows and three calves were treated intramuscularly with an oxytetracycline (otc) preparation. the dose was about 5 mg/kg. the animals were slaughtered eight days after the treatment and their kidneys and muscle samples were taken for analysis. twelve cows were treated intramammarily with an otc preparation 12 hours before slaughter. six of the cows were treated in two udder quarters with a preparation containing 200 mg otc chloride per dose; the other six were similarly treated in four ... | 1976 | 1012914 |
| [nature of tryptophan photooxidation products in lysozyme in the presence of methylene blue]. | one out of six trytophan residues in two lysozyme modification, obtained under lysozyme photooxidation in the presence of methylene blue, is found to be oxidized to n'-formylkinurenine (in one modification) and to kinurenine (in the other modification). the transition of one modification into another via detaching of n'-formyl group by soft acid hydrolysis has shown that one and the same tryptophan residue is oxidized in both products, possible mechanism of tryptophan oxidation to the products m ... | 1976 | 1024586 |
| effect of experimental dermatophyte infection on cutaneous flora. | the cutaneous aerobic bacterial flora was monitored during the course of experimental dermatiphyte (ringworm) infections on the forearms of 9 volunteers. micrococcaceae were identified by the new baird-parker classification with the aid of a replica-plating technique. there were significant differences in total populations but not in kinds of flora compared with control (opposite) forearms. the proportion of penicillin-resistant microorganisms, however increased the infection, to a degree which ... | 1975 | 1039027 |
| physico-chemical and biological study of excision-repair of uv--irradiated phix174 rf dna in vitro. | we have studied excision-repair of uv-irradiated phix174 rfi dna in vitro with uv-specific endonuclease from micrococcus luteus (uv-endo), dna polymerase i from escherichia coli and dna ligase from phage t4 infected e. coli. excision-repair was measured a) by physico-chemical methods, i.e. by determination of the conversion of rf i dna into rf ii dna by uv-endo and by the subsequent conversion of rf ii dna ligase, b) by biological methods i. e. by measuring the ability of the reaction product to ... | 1975 | 1052535 |
| identification of the cultivable bacteria in dental plaque from the beagle dog. | fifteen different genera of microorganisms were isolated from the 27-month-old plaque of two beagle dogs. they were as follows: pseudomonas, proteus, neisseria, escherichia, staphylococcus, streptococcus, lactobacillus, nocardia, actinomyces, corynebacterium, micrococcus, microbacterium, brevibacterium, arthrobacter, and kurthia. the dog plaque was characterized by a low number of streptococci and the presence of several noncarbohydrate-fermenting organisms. | 1976 | 1069758 |
| affinity chromatographic purification of human lysozyme, with special reference to human leukemia lysozyme. | lysozyme [ec 3.2.1.17] was purified from human tears, serum, and urine of acute monocytic leukemia patients, renal disease patients, and residents in cadmium-polluted areas of tsushima island using an affinity adsorbent containing lysozyme-lysate of micrococcus lysodeikticus cell walls as the ligand. by means of this procedure, leukemia lysozyme was purified 100- to 200-fold with an activity recovery of 80%. it was crystallized at ph 10. this purified preparation appeared homogeneous in disc ele ... | 1976 | 1070487 |
| [contribution to the study of staphylococcus isolated of the mouth. iv. staphylococcus isolated from infected root canals]. | | 1975 | 1073589 |
| kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase. | the kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of micrococcus luteus has been investigated. double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either substrate is unaffected by the presence of the other. dadp, an analogue of adp product, is a competitive inhibitor with respect to pi and polynucleotid ... | 1975 | 1078670 |
| [role of dna-membrane attachment in repair of radiation damage in micrococcus radiodurans]. | the unirradiated bacterial dna assciated with the membrane is liberated into the cytoplasm after breakage of either a single or a double strand, resulting from x-ray action. during the reincubation period in growth-medium, the dna is reassociated with the membrane. this phenomenon is very rapid and occurs without increasing the molecular weight of dna. the study of dna-membrane complexes shows that the size of the dna-associated membranous fragment differs according to the lysing technique emplo ... | 1975 | 1079020 |
| the inhibition in vitro of dna polymerase and rna polymerases by daunomycin and adriamycin. | | 1975 | 1089410 |
| kinetic study on phagocytosis of bovine leukocytes measured by oxygen uptake. | | 1975 | 1091506 |
| effects of human and rabbit serum on viability, permeability, and envelope lipids of serratia marcescens. | the major action of serum on gram-negative organisms is thought to be on the microbial envelope. we compared the effects of normal human and rabbit serum on the envelope lipids of two strains of serratia marcescens, one sensitive and one resistant to the bactericidal effects of serum. during killing by either serum, the sensitive strain underwent rapid permeability changes coincident with degradation of microbial phospholipids. the resistant strain exhibited none of these effects. the phospholip ... | 1975 | 1091552 |
| comparative ability of rna and dna to prime dna synthesis in vitro: role of sequence, sugar, and structure of template-primer. | the priming efficiency of oligo(rna) vs. oligo(dna) in a homopolymer template-homooligomer primer system was compared using four dna polymerases. the templates included (dt)n, (da)n, (dc)n, and (di)n. primers were the oligomers of the complementary dna or rna with chain lengths of 6 to 23. the dna polymerases used were from micrococcus luteus, avian myeloblastosis virus (amv), and escherichia coli (polymerase i and polymerase iii). the polymerases demonstrated a preference for the dna primers wi ... | 1975 | 1092334 |
| conformations and structural transitions in polydeoxynucleotides. | polydeoxynucleotides of different base sequence, the alternating poly[d(a-t)]-poly[d(a-t)], crab satellite dna, on the one hand, and double-stranded homopolymer complexes poly[d(a)]-poly[d(t)], poly[d(i)]-poly[d(c)], on the other, display significant differences in their conformation and conformational transitions. infrared linear dichroism investigations indicate that the alternating poly[d(a-t)]-poly[d(a-t)], enzymatically synthesized, adopts a lower humidity a well-expressed a* form in which ... | 1975 | 1092345 |
| ultraviolet reactivation and ultraviolet mutagenesis of infectious lambda dna: strong inhibition by treatment of dna in vitro with uv-endonuclease from micrococcus luteus. | uv-endonuclease from microcossuc luteus induces single-stranded breaks in uv-irradiated dna of phage lambda and the average length of the fragments produced (after uv-doses to dna of 135 and 675 erg/mm2) is equal to the average spacing between pyrimidine dimers. the plaque-forming ability of uv-irradiated lambda dna used to infect ca++-treated uvr a6, uvrb5 or uvrc34 recipient escherichia coli cells (but not uve+ cells) may be significantly enhanced by treatment of lambda dna with uv-endonucleas ... | 1975 | 1093009 |
| (da-dt) dependent inactivation of the dna template properties by interaction with netropsin and distamycin a. | the inhibitory effect of the polypeptide antibiotics netropsin and distamycin a on dna dependent nucleic acid synthesis has been shown to be related to the base composition of the template dna. a number of natural dna's of quite different da-dt content as well as poly (di-dc)-poly (di-dc), poly (da-dt)-poly (da-dt), poly (da) - poly (dt) and poly (dg) - poly (dc) has been studied as templates in dna and in part in rna polymerase reaction. the highest binding efficiency of netropsin existing for ... | 1975 | 1093141 |
| separation of nucleotide diphosphokinases from e. coli dna polymerase. | | 1975 | 1093210 |
| studies of the mechanisms of radiosensitization of bacterial and mammalian cells by diamide. | diamide sensitizes bacterial and mammalian cells to radiation by at least two mechanisms. sensitization of v79-gl1 chinese hamster cells is due mainly to a reduction of a reduction of the survival-curve shoulder, is observed both in oxygen and in hypoxia,and is additive to the sensitization of hypoxic cells by some nitroimidazoles. in contrast, sensitization of the radioresistant organism, micrococcus sodonensis, which has apronounced shoulder, is entirely dose-modifying. in a rapid-mix study us ... | 1975 | 1093986 |
| the activation of rna polymerase by alkylammonium ions. | tetramethylammonium and tetraethylammonium ions both lower the melting temperature of dna. we have shown that these ions increase the activity of e. coli rna polymerase. when the base composition of the dna template was varied, there was a correlation between the decrease in melting temperature and increase in rna polymerase activity. no stimulation was observed when heat denatured dna was used as template. it was shown that initiation of rna synthesis was stimulated but to a smaller degree than ... | 1975 | 1094422 |
| enzymatic repair of dna. | | 1975 | 1094910 |
| proceedings: continuous-culture studies of bacterial interactions among human skin commensals. | | 1975 | 1095745 |
| microbiological characteristics of dungeness crab (cancer magister). | aerobic, heterotropic microorganisms of dungeness crab (cancer magister) were isolated from raw crab, cooked crab, crab meats obtained during commercial processing, and from retail crab meat samples. each microbial isolate was then identified to the genus level employing the revised replica plating procedure. microbial groups most commonly isolated from crab meat were, in the order of predominance, moraxella, pseudomonas, acinetobacter, arthrobacter, micrococcus, flavobacterium-cytophaga, and ba ... | 1975 | 1096824 |
| on the covalent binding of mrna models to the part of the 16 s rna which is located in the mrna binding site of the 30 s ribosome. | | 1975 | 1096895 |
| synthesis of a chemically reactive analog of the initiation codon: its reaction with ribosomes of escherichia coli. | nitrophenylated 5'-adenylic acid could be employed as primer in a polyribonucleotide nucleotidyltransferase (micrococcus luteus) reaction to yield 5'-nitrophenylated pa-u-g. after reduction and subsequent bromoacetylation, an a-u-g analog was obtained, which could be used as an affinity label for the ribosomal a-u-g-binding site(s). after incubating the a-u-g affinity label with 70s ribosomes, 30s subunits programmed for initiation-factor-dependent fmet-trnametf binding were obtained. hence, the ... | 1975 | 1097317 |
| [bacteriological and parasitological investigations in public indoor swimming baths (author's transl)]. | the importance of the public swimming bath for the dissemination of trichomonas vaginalis is disputed. 30 samples, each of 250 ml bath water from four indoor swimming baths, were examined for trichomonads. all the samples were negative. 218 smears taken from the wet places on the heated benches and other seating accommodation in these swimming baths were cultured for trichomonads and bacteria. trichomonas vaginalis could not be demonstrated. fecal organisms were found in 56 places, antibiotic-se ... | 1975 | 1098331 |
| [ecology of the obligate thermophilic bacteria]. | the obligate thermophilic non-sporeforming bacterium thermus ruber, isolated from the thermal springs of kamchatka at temperatures of 60 to 90 degrees c (loginova et al.,1974), has a high lytic activity towards the living and dead cells of escherichia coli and micrococcus lysodeikticus. | 1975 | 1099409 |