| laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract. | the technique of isolating chlamydia trachomatis from the human gential tract by centrifuging clinical specimens on to cell monolayers with subsequent incubation has been improved and simplified. gentamicin in the media was found to be superior to streptomycin in reducing bacterial contamination of specimens. the infectivity of chlamydial suspensions of laboratory cultured material was significantly reduced by storage at +4 degrees c for more than 48 hours, and by immediate freezing to -70 degre ... | 1975 | 1236632 |
| [morphological ultrastructural and immunological studies of a rickettsia isolated from a parrot (author's transl)]. | a rickettsia isolated from a parrot was found by use of electron microscopic and serological technic to be a coxiella burneti. in cell culture one can see a dramatic polymorphism and a gram negative-like structure of the cell wall. they divide by binary fission with no appearance of a complexe cycle of development like the one described for chlamydia psittaci. many myelinic fibrils and altered coxiella suggest that the host cell react very deaply. | 1975 | 1238044 |
| ultrastructural studies of chlamydia psittaci 6bc in situ in yolk sac explants and l cells: a comparison with gram-negative bacteria. | chlamydia psittaci (6bc) was grown in yolk sac explants and in l cells and fixed by perfusion in situ to provide undamaged material for comparison with gram-negative bacteria. reticulate, intermediate, and elementary bodies were all seen to lack a well-defined periplasmic space; intermediate and elementary bodies showed condensations of the nucleoid which differ from common bacterial configurations; and the cytoplasm of highly condensed elementary bodies was much more electron dense than that of ... | 1975 | 1238156 |
| the effect of purification on the ultrastructure and infectivity of egg-attenuated chlamydia psittaci (6bc). | a procedure is described for the purification of mixed populations of the three different morphological forms of chlamydia psittaci (6bc) from infected yolk sac membranes. elementary bodies and small intermediate bodies are not perceptibly damaged during purification which involves homogenization of the host cells, differential centrifugation, sedimentation through 20% sucrose, and treatment with trypsin. the observation that elementary bodies undergo plasmolysis in 20% sucrose is interpreted as ... | 1975 | 1238157 |
| comparative infectivity of trachoma organisms in hela 229 cells and egg cultures. | thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in hela 229 cells pretreated with diethylaminoethyl-dextran to determine the relative sensitivity of the two culture systems for detection of the infection. the strains tested were both yolk sac- and hela cell-established isolates from the eye and the genital tract. the study showed that the cell culture was of equal or greater sensitivity than yolk sac culture and that ocular and genital iso ... | 1975 | 1238370 |
| morphological picture of testes of bulls infected with microorganisms of the genus chlamydia. | | 1975 | 1240018 |
| letter: chlamydial infections. | | 1975 | 1242188 |
| isolation of chlamydia from women attending a clinic for sexually transmitted disease. | cervical swabs for chlamydia culture were collected from 638 unselected women attending a sexually transmitted diseases clinic with a fresh complaint. chlamydia were isolated from 76 (12 per cent.) of the women. when the results were related to the patients' diagnoses, chlamydia were present in 44 per cent. of women with gonorrhoea and in 22 per cent. of women who were contacts of men with nonspecific urethritis (women who may be regarded as having non-specific genital infection). chlamydia were ... | 1975 | 1242683 |
| some aspects of sexually transmitted diseases today. | | 1975 | 1242807 |
| trachoma organisms: technical advances in laboratory diagnosis. | new knowledge of the microbiology and immunology of chlamydia is reviewed. new serological and isolation methods for diagnosis of trachoma infection are described. results of studies of ocular and genital trachoma infection utilizing these new techniques are presented. | 1975 | 1243119 |
| medical aspects of some zoonoses. | | 1975 | 1243682 |
| the human placenta as a possible reservoir of chlamydial infection in northern canada. | | 1976 | 1260630 |
| ovine chlamydial abortion in alberta. | | 1976 | 1260643 |
| urethritis associated with chlamydia: clinical and laboratory diagnosis. | | 1976 | 1272211 |
| experimental host immune response in respiratory chlamydiosis. | | 1976 | 1277086 |
| an assessment of the extent of blindness in machakos district. | | 1976 | 1278077 |
| the characterization of nonbacterial prostatitis: search for an etiology. | nonbacterial prostatitis is often difficult to differentiate from other prostatic complaints and remains a vaguely characterized syndrome. prostatic fluid inflammatory cells and elevated immunoglobulins raise the suspicion that this syndrome is caused by some undetected infection. prostatic fluid antibodies against chlamydia trachomatis, ureaplasma urealyticum, staphylococcus, staphylococcus faecalis, bacteroides fragilis and clostridium perfringens were measured in men with nonbacterial and bac ... | 1992 | 1279213 |
| azithromycin-induced block of elementary body formation in chlamydia trachomatis. | the mechanism of action of azithromycin on the murine strain of chlamydia trachomatis grown in tissue culture epithelial cells is addressed. azithromycin at a concentration of 100 ng/ml inhibits chlamydial growth in tissue culture, a value that agrees well with prior in vitro data from human strains of c. trachomatis grown in tissue culture. by morphological criteria, the block to chlamydial growth appears to occur early in its life cycle. azithromycin is not directly toxic to chlamydial element ... | 1992 | 1280057 |
| azithromycin. a review of its antimicrobial activity, pharmacokinetic properties and clinical efficacy. | azithromycin is an acid stable orally administered macrolide antimicrobial drug, structurally related to erythromycin, with a similar spectrum of antimicrobial activity. azithromycin is marginally less active than erythromycin in vitro against gram-positive organisms, although this is of doubtful clinical significance as susceptibility concentrations fall within the range of achievable tissue azithromycin concentrations. in contrast, azithromycin appears to be more active than erythromycin again ... | 1992 | 1280567 |
| infection with a plasmid-free variant chlamydia related to chlamydia trachomatis identified by using multiple assays for nucleic acid detection. | clinical samples in transport media from 40 patients exhibiting pathologies potentially caused by chlamydia trachomatis infection were analyzed for chlamydial nucleic acid, and the results were compared with those of culture. chlamydial culture was performed by a shell vial centrifugation method with hela 229 host cells. polymerase chain reaction (pcr) assays were used to detect either regions on a 7.5-kb plasmid characteristic of c. trachomatis (plasmid-pcr) or a segment of the 16s rrna genes ( ... | 1992 | 1280642 |
| sexually transmitted diseases and hiv infection. | | 1992 | 1285204 |
| sexually transmitted diseases in england and wales: 1981-1990. | data provided by genito-urinary medicine clinics, microbiology laboratories and other reports reveal a consistent picture of the epidemiology of sexually transmitted diseases in england and wales during the 1980s. the number of new cases increased by 21% between 1981 and 1990; most of this increase being due to genital herpes, genital warts and 'non-specific genital infection' (including that due to chlamydia trachomatis). the increase in attendance rates for new cases was much higher for women ... | 1992 | 1285223 |
| expression of a chlamydia anticarbohydrate single-chain antibody as a maltose binding fusion protein. | a single-chain antibody fragment has been constructed for an antibody that binds to the chlamydia specific carbohydrate structure of the lipopolysaccharide. single-chain protein was expressed and secreted into the periplasmic space of e. coli as a fusion protein with the maltose binding protein. the fusion protein was purified in one step by virtue of its ability to bind to maltose. in a sandwich elisa, the eluted protein bound chlamydia lipopolysaccharide, which demonstrates that the single-cha ... | 1992 | 1285321 |
| the role of the laboratory in a chlamydia control programme in a developing country. | the laboratory components of a chlamydia trachomatis disease control programme for a developing country are reviewed. early diagnosis of chlamydial infections is the most cost effective means of preventing the long term sequelae of trachoma, pelvic inflammatory disease, ectopic pregnancy and infertility, which are now a major public health burden to the health care system in developing countries. public health strategies are required to establish both a co-ordinated limited system of laboratory ... | 1992 | 1286634 |
| changes in the spectrum of organisms causing respiratory tract infections: a review. | over the last decade, the spectrum of organisms causing community-acquired acute lower respiratory tract infections has changed. streptococcus pneumoniae now causes approximately 30% of outpatient acute pneumonia-less than in former decades-whereas mycoplasma pneumoniae is found in both young and elderly patients. the enterobacteriaceae and staphylococcus aureus are now seen more frequently as respiratory tract pathogens in community-acquired pneumonia patients, and they are the major organisms ... | 1992 | 1287613 |
| chlamydial infections in selected populations in jamaica. | the importance of chlamydia trachomatis as a cause of genital tract infection in caribbean countries is largely unknown. two hundred and sixteen cervical and urethral specimens were collected from patients with cervicitis and urethritis attending a sexually transmitted diseases (std) clinic, a gynaecology clinic and a family planning clinic in kingston. specimens were tested for chlamydiae by direct immunofluorescence and cell culture methods. they were also investigated for n. gonorrhoeae infec ... | 1992 | 1290231 |
| [uses of vaginal ph measurement for prevention of premature labor. results of a prospective study]. | the influence of the microflora of the vagina and cervix has been undervalued for the induction of premature birth. examination of the microbiological problem by determination of the vaginal ph in combination with the microflora (chlamydia trachomatis, mycoplasma hominis, ureaplasma urealyticum). report of the outcome of 161 prospective, ad the beginning normal pregnancies in comparison of 200 primary hospitalized pregnancies with symptoms of imminent premature birth. conclusion: the vaginal ph ... | 1992 | 1290278 |
| laboratory reports of human viral and selected non-viral infections in canada--1991. | | 1992 | 1291004 |
| detection of igm antibodies to chlamydia trachomatis, chlamydia pneumoniae, and chlamydia psittaci from japanese infants and children with pneumonia. | chlamydia trachomatis (c. trachomatis) is now well established as a pathogen of neonatal inclusion conjunctivitis and infantile pneumonia. c. pneumoniae (twar) and c. psittaci also cause pneumonia and other respiratory infections. serum samples from 223 japanese infants and children with pneumonia were tested for igm antibodies to c. trachomatis, c. pneumoniae and c. psittaci. igm antibodies to c. trachomatis were measured by an enzyme immunoassay (eia) and by a microimmunofluorescence (mif) tes ... | 1992 | 1296808 |
| [antibiotic therapy in bronchopulmonary infections]. | because of difficulties in accurately determining an etiologic diagnosis, the ideal treatment for lower respiratory tract infections remains questionable. suggested regimens are made on the basis of clinical and epidemiological data. however, the single most common pathogen responsible for pneumonia remains streptococcus pneumoniae. atypical pneumonia in younger patients is best treated with macrolides. older patients without debility or immunodepression are best treated with amoxycillin-ampicil ... | 1992 | 1297403 |
| dna fingerprinting of chlamydia trachomatis by use of ribosomal rna, oligonucleotide and randomly cloned dna probes. | dna fingerprinting of 15 reference strains and 24 clinical isolates of chlamydia trachomatis, 2 strains of c. psittaci and one strain of c. pneumoniae was studied by use of universal 16 + 23s rna from escherichia coli, 16s rdna-directed oligonucleotide and randomly cloned chlamydial dna probes. the rrna-gene restriction patterns (ribotypes) enabled the differentiation of chlamydial species. following dna cleavage by restriction endonuclease pvuii, lymphogranuloma venereum and trachoma biovars of ... | 1992 | 1298028 |
| [study on the infections of u. urealyticum, m. hominis, and c. trachomatis in patients with venereal diseases and healthy controls in three areas of china]. | two hundred and thiry-nine patients with venereal diseases and 264 healthy controls, from yichang, liuzhou, and beijing, were detected for the antibodies to ureaplasma urealyticum (uu). mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemagglutination (iha) in 1990. the results showed that: 1) in patients with venereal diseases, the positive rates was the highest in ct (27.62%), higher in uu (17.57%), and lower in mh (4.18%); 2) the levels of antibody to ct and uu were signifi ... | 1992 | 1301266 |
| [long lasting conjunctivitis: research of etiological factors]. | forty children with long-lasting or recurrent conjunctivitis were included in this etiological study. it is well known that purulent conjunctivitis is mainly bacterial, with a major source of infection from chlamydia; recently, however, a greater percentage of viral forms, with the exception of conjunctivitis without secretion, has been reported. the authors focused their attention on the clinical symptoms and on bacteriological studies of the forms of chlamydia and mycoplasma conjunctivitis, hi ... | 1992 | 1301484 |
| characteristics of women by smoking status in the san francisco bay area. | to better understand why smokers are more likely to develop cervical cancer than nonsmokers, we investigated laboratory and demographic differences between the two groups. women between the ages of 18 and 49 who attended eleven community clinics in the san francisco bay area were studied to investigate differences between smokers and nonsmokers. the 332 smokers and 365 nonsmokers were queried about smoking habits, sexual and reproductive history, and recent diet. cervical mucus specimens were cu ... | 1992 | 1302562 |
| [prevalence in tucumán of chlamydia trachomatis and ureaplasma urealyticum in sexually active women]. | a group of 298 sexually active women who consulted for cervicitis, sterility or infertility were studied to detect the prevalence of chlamydia trachomatis and ureaplasma urealyticum and also to correlate the presence of these pathogens with tube damage. chlamydial antigens were detected with a fluorescent commercial reagent with specific monoclonal antibodies. the isolation of u. urealyticum was made following the shepard and lunceford (19) techniques. in all samples of endocervical canal, we ob ... | 1992 | 1302867 |
| chlamydia pneumoniae respiratory infection in a child--a case report. | a case of respiratory infection in a child due to chlamydia pneumoniae is reported. the diagnosis was made by the detection of chlamydial antigen in the tracheal secretion and a significant increase in c. pneumoniae antibody titre. the infection responded well to erythromycin therapy. | 1992 | 1303485 |
| purification of chlamydia psittaci antigen by affinity chromatography on polymyxin b agarose for use in the enzyme-linked immunosorbent assay (elisa). | the purification of cell wall antigens of chlamydia psittaci by affinity chromatography on polymyxin b agarose is described. chlamydial cell wall antigens were prepared using different methods: heat treatment, ultrasonication and sodium deoxycholate treatment. the antigens were subsequently purified by gel chromatography. the highest amount of cell wall antigens was obtained by heat treatment of the chlamydiae at 90 degrees c and ph 8.5. the purified antigens showed molecular weights of 450 kda ... | 1992 | 1303687 |
| evaluation of the polymerase chain reaction (pcr) for detection of chlamydia psittaci in abortion material from ewes. | the polymerase chain reaction (pcr) was evaluated as a diagnostic tool for detection of chlamydia (c.) psittaci in abortion material from 40 ewes. for this purpose, pcr results of 87 samples were compared with direct microscopic identification after chemical staining, cell culture isolation and a commercially available enzyme-linked immunosorbent assay (elisa). the value for sensitivity as compared to cell culture was 97.7% whereas the specificity-value was calculated to be 84.1%. | 1992 | 1303688 |
| [threats of infectious factors in the uterine neck regarding chlamydia trachomatis in imminent premature deliveries]. | the research concerned threats of infections factors to a endocervix in imminent premature deliveries. it was ascertained that chlamydia (c) trachomatis is the most frequent pathogenic factor, which occurred in 41.8% of cases. the premature delivery occurred in 16.3% of women with positive c. trachomatis test results. | 1992 | 1303914 |
| in situ hybridisation: principles and applications. | in situ hybridisation (ish) is based on the complementary pairing of labelled dna or rna probes with normal or abnormal nucleic acid sequences in intact chromosomes, cells or tissue sections. compared with other molecular biology techniques applicable to anatomical pathology, ish enjoys better rapport with histopathologists because of its similarity to immunohistochemistry. it has the unique advantage over other molecular biology techniques--largely based on probe hybridisation with nucleic acid ... | 1992 | 1304627 |
| [infection with chlamydia trachomatis among women with infertility treatment]. | 68 women with sterility treatment were tested. among 13 of them (20%) presence of chlamydial antibodies was found. hormonal profile was estimated. in a group of women with positive serologic reaction increased concentration of prolactin was found. | 1992 | 1305128 |
| serological survey for bovine bacterial and viral pathogens in captive arabian oryx (oryx leucoryx pallas, 1776). | tests for antibodies to bovine bacterial and viral pathogens were conducted on 239 sera from 128 arabian oryx (oryx leucoryx) from seven locations (taif, riyadh and mahazat as said, saudi arabia; san diego, united states of america [usa]; shaumari, jordan; qatar; and bahrain). no antibodies to pasteurella multocida type e or epizootic haemorrhagic disease 1 virus were found. antibodies to brucella abortus, p. multocida type b, p. multocida type d, lumpy skin disease virus and akabane virus were ... | 1992 | 1305861 |
| the efficacy of clarythromycin (a-56268, te-031) in the treatment of genital chlamydial infection. | the authors report the preliminary results of an experience of treatment with clarythromycin in chlamydia trachomatis endocervicitis/endourethritis; 100% of 51 outpatients treated had negative elisa results 7-10 days after the end of treatment with clarythromycin 500 mg. b.i.d. for 7 days. among 64 female outpatients with chlamydial cervicitis treated with erythromycin 1 g b.i.d. for 7 days, 88% were negative at elisa at the same intervals after therapy. the authors conclude that the efficacy of ... | 1992 | 1306155 |
| iga antichlamydia antibodies as a diagnostic tool for monitoring of active chlamydial infection. | chlamydia trachomatis isolation in culture is the best means of chlamydial infection diagnosis. antichlamydial antibodies, if present, may also serve as diagnostic markers. in eleven patients with positive c. trachomatis culture and serological positive response, specific iga and igg antibody titers were measured before and after treatment. in all cases, cultures became negative after treatment and iga titers decreased rapidly, while igg levels remained high. thus, presence of specific iga antib ... | 1992 | 1294398 |
| the 'other' sexually transmitted diseases. chlamydial, herpes simplex virus, and human papillomavirus infections. | the incidence of sexually transmitted diseases continues to rise across the country. because many cases are subclinical and asymptomatic, chlamydial, herpes simplex virus, and human papillomavirus (hpv) infections continue to spread at an alarming rate. these infections can lead to serious sequelae, such as pelvic inflammatory disease, infertility, and cervical dysplasia or carcinoma, so improved disease-control strategies are needed. preventive efforts should include use of the latest diagnosti ... | 1992 | 1312236 |
| clarithromycin and azithromycin: new macrolide antibiotics. | the chemistry, mechanism of action, antimicrobial spectrum, pharmacokinetics, clinical efficacy, adverse effects, drug interactions, and dosage and administration of clarithromycin and azithromycin are described. clarithromycin and azithromycin are new macrolide antibiotics that are similar in structure to erythromycin. compared with erythromycin, clarithromycin demonstrates increased activity against staphylococcus aureus, streptococci, legionella pneumophila, moraxella catarrhalis, and chlamyd ... | 1992 | 1312921 |
| postoperative spindle-cell nodule of urinary bladder with unusual intracytoplasmic inclusions. | postoperative spindle-cell nodule of the urinary bladder (or in the absence of previous surgical procedure, inflammatory pseudotumor) is a reactive process of unknown etiology mimicking a sarcoma. intracytoplasmic inclusion bodies were noted inside the atypical spindle cells on diff-quik stained smears in a recent case of this entity. although fluorescence was noted in the inclusion bodies with monoclonal antibodies against the major outer membrane protein of the chlamydia species, the exact nat ... | 1992 | 1314731 |
| comparative evaluation of clindamycin/gentamicin and cefoxitin/doxycycline for treatment of pelvic inflammatory disease: a multi-center trial. the european study group. | the clinical efficacy and safety of clindamycin-gentamicin versus doxycycline-cefoxitin in the treatment of acute pelvic inflammatory disease was evaluated in a comparative, randomized, prospective, multicenter study. ten investigators enrolled 170 patients. those judged to be eligible for efficacy were 60/88 (68%) who received the clindamycin-gentamicin regimen and 55/82 (67%) of those treated with cefoxitin-doxycycline. a successful clinical outcome was attained for 52/60 (87%) and 46/55 (84%) ... | 1992 | 1316041 |
| ultrasound findings in perihepatitis associated with pelvic inflammatory disease. | the width of the right anterior extrarenal tissue was increased on ultrasound examination in 9 patients with perihepatitis (fitz-hugh-curtis syndrome). normal values were obtained in 72 women without detectable or known disease and were found to be between 1 mm and 5 mm (mean 1.8 mm). the 9 patients with laparoscopically verified perihepatitis who demonstrated this thickening had values ranging from 6 mm to 12 mm, (mean 7.7 mm). although the incidence of this inflammatory thickening in perihepat ... | 1992 | 1316376 |
| updates on chlamydia. | | 1992 | 1317033 |
| in vitro and in vivo activities of sparfloxacin, other quinolones, and tetracyclines against chlamydia trachomatis. | sparfloxacin was more potent than other quinolones (tosufloxacin, lomefloxacin, ciprofloxacin, ofloxacin, fleroxacin, enoxacin, and norfloxacin) and as potent as minocycline and doxycycline in activity against chlamydia trachomatis in vitro and in vivo. sparfloxacin was more bactericidal than minocycline against c. trachomatis d/uw-3/cx. | 1992 | 1317145 |
| [intrauterine infections: frequency and diagnosis]. | intrauterine infections occupy a considerable place in human pathology--a conclusion drawn on the basis of original and literature data. herpetic, cytomegalic, mycoplasma and chlamydial infections are the most important among them. intrauterine infections may be the cause of some diseases considered as somatic ones. the possibility of the pathology diagnosis of these disease not only on the basis of laboratory data but on the basis of characteristic structural changes of cells and tissues is sho ... | 1992 | 1317699 |
| development of a monoclonal antibody-based p24 capsid antigen detection assay for htlv-i, htlv-ii, and stlv-i infection. | a monoclonal antibody-based antigen capture enzyme-linked immunosorbent assay (elisa) was developed and employed to detect p24 capsid antigen from human t-cell lymphotropic viruses type i and ii (htlv-i, htlv-ii), simian t-cell lymphotropic virus type i (stlv-i)-infected cell lines, and from mononuclear cell cocultures of htlv-infected humans and stlv-i infected monkeys. a monoclonal antibody specific for htlv p24 and p53 capsid antigens was coated onto 96-well microtiter plates to capture htlv/ ... | 1992 | 1318063 |
| antibacterial activity in vitro of cefpirome against clinical isolates causing sexually transmitted diseases. | the in-vitro activity of cefpirome was compared with other antibiotics against organisms causing sexually transmitted diseases (std). the excellent activity of cefpirome against neisseria gonorrhoeae (mic90 1.0 mg/l), haemophilus ducreyi (mic90 0.5 mg/l), and gardnerella vaginalis (mic90 1.0 mg/l) suggests that this agent might be useful in the empirical treatment of a variety of venereal diseases. | 1992 | 1318294 |
| in vitro evaluation of activities of azithromycin, erythromycin, and tetracycline against chlamydia trachomatis and chlamydia pneumoniae. | the in vitro activities of azithromycin (cp-62,993; pfizer), erythromycin, and tetracycline were evaluated by inhibiting chlamydia trachomatis and chlamydia pneumoniae, formerly twar, propagation in vitro in mccoy cells, hela cells, and hl cells. eleven clinical isolates of c. trachomatis (serovars d, e, f, j, k, and l2) and four strains of c. pneumoniae were tested with an inoculum of 10(3) inclusion-forming units in a 96-well microtiter plate. the mic ranges of these antimicrobial agents again ... | 1992 | 1318677 |
| infections of the cervix uteri with human papillomavirus and chlamydia trachomatis. | apart from infection with human papillomavirus (hpv), other microorganisms may be involved in the development of cervical neoplasia. to study concomitant infections with hpv and chlamydia trachomatis, cervical specimens from 4 groups of women were examined for the presence of these microorganisms by the polymerase chain reaction. the first group consisted of 143 consecutive samples from women with no cytological abnormalities who participated in a triennial screening program to prevent cervical ... | 1992 | 1320098 |
| in vitro activities of five quinolones against chlamydia pneumoniae. | the in vitro susceptibilities of 10 strains of chlamydia pneumoniae were determined for five quinolones, including ciprofloxacin, ofloxacin, fleroxacin, temafloxacin, and sparfloxacin. sparfloxacin was the most active compound tested, followed by ofloxacin and temafloxacin. ciprofloxacin and fleroxacin were the least active. the use of hep-2 cells for testing c. pneumoniae resulted in larger inclusions but essentially the same endpoints as were seen with use of hela 229 cells. | 1992 | 1320366 |
| the in-vitro activity of two new quinolones: rufloxacin and mf 961. | the in-vitro activity of two new quinolone antimicrobials, rufloxacin and mf 961, together with the desmethylated metabolite of rufloxacin (mf 922) were compared with other orally administered agents against 622 bacterial strains. against enterobacteriaceae and pseudomonas aeruginosa rufloxacin was generally active (mic90 1-8 mg/l) with the exception of klebsiella and serratia spp. (mic90 32 mg/l and enterobacter spp. (mic90) 64 mg/l. the respiratory pathogens haemophilus influenzae and moraxell ... | 1992 | 1324239 |
| in vitro activity of nonoxynol 9 on hela 229 cells and primary monkey cervical epithelial cells infected with chlamydia trachomatis. | nonoxynol 9 (non-9) is the active ingredient in a wide variety of vaginal contraceptive preparations. the manufacturer recommendation for optimal contraceptive practice is repeated application every 6 h. we studied the in vitro activity of non-9 against chlamydia trachomatis (e/uw-5/cx) and its toxicity against hela 229 cells and monkey cervical epithelial cells. with a contact time of 6 h, non-9 was toxic to hela cells at concentrations of 50 micrograms/ml or greater and to monkey cervical cell ... | 1992 | 1324646 |
| in vitro activities of azithromycin, clarithromycin, l-ofloxacin, and other antibiotics against chlamydia pneumoniae. | the in vitro susceptibilities of 11 strains of chlamydia pneumoniae to azithromycin, clarithromycin, erythromycin, l-oflaxacin, and doxycycline were determined. clarithromycin was the most active agent tested, with an mic for 90% of strains and minimal chlamydiacidal concentration for 90% of strains of 0.03 microns/ml. the activity of azithromycin was similar to those of erythromycin and doxycycline, with mics for 90% of strains of 0.125 to 0.25 microns/ml. however, the prolonged half-life and e ... | 1992 | 1324650 |
| a controlled trial of a single dose of azithromycin for the treatment of chlamydial urethritis and cervicitis. the azithromycin for chlamydial infections study group. | currently, there is no single-dose therapy that is effective in the treatment of urethral or endocervical infections with chlamydia trachomatis. azithromycin is a new azalide antibiotic that has substantial activity against c. trachomatis, is concentrated intracellularly, and has a long half-life in serum and tissue. | 1992 | 1325036 |
| [pneumopathies caused by chlamydia pneumoniae]. | among the atypical pneumonias observed between march 1990 and march 1991, 6 were diagnosed as being caused by chlamydia pneumoniae of the twar strain. the serological diagnosis was obtained by a microimmunofluorescence test. all 6 patients had anti-twar antibody levels higher than 512; they were treated with a macrolide administered by the oral route and were cured without sequelae or recurrences. four cases received a ten day course of roxithromycin 300 mg/day and one case received erythromycin ... | 1992 | 1326110 |
| clinical course of cervical human papillomavirus lesions in relation to coexistent cervical infections. | a prospective follow-up of 530 women with cervical human papillomavirus (hpv) infection was conducted from 1981 to the present (mean 62.9 months). the patients were examined by pap smears and colposcopy with or without biopsies every sixth month. endocervical swabs were taken for culture of cytomegalovirus (cmv), herpes simplex virus (hsv), and chlamydia trachomatis at each visit. during the follow-up period, 179 of the 530 patients (33.8%) had cervical infection and 351 (66.2%) had no coexisten ... | 1992 | 1326128 |
| survey of the treatment of chlamydia trachomatis infection of the female genital tract. | a questionnaire was sent to every tenth (n = 302) general practitioner in norway. the physicians were to indicate their choice of antibiotic regimen for the treatment of genital chlamydial infections in women. sixty-nine percent of the practitioners responded. the choice of treatment varied widely between the respondents. forty-two different regimens were used for chlamydial cervicitis, 34 for cervicitis in pregnancy and 63 for probable pelvic infection. of the prescribed treatments for these th ... | 1992 | 1326211 |
| dietary habits, prepregnancy weight, and weight gain during pregnancy. risk of pre term rupture of amniotic sac membranes. | premature rupture of amniotic sac membranes (prom) is associated with perinatal morbidity and mortality. a matched case-control study was undertaken to determine whether dietary habits, prepregnancy weight, and weight gain during pregnancy were associated with increased risks for prom. | 1992 | 1326841 |
| [genital infections with chlamydia trachomatis in pregnant women in new caledonia]. | chlamydia trachomatis was researched from 400 pregnant women. 76 of them were found infected and 129 carried serological signs of infection. we purpose a detection at the end of pregnancy interesting women with sexually transmitted diseases background and those with serological positive results (greater than 1/64). then, treatment of the mother will be an useful method of prevention against neonatal infection. | 1992 | 1327347 |
| the expanding clinical spectrum of infections with chlamydia trachomatis. | | 1977 | 929347 |
| antibiotic susceptibility of chlamydia trachomatis. | the antibiotic susceptibility of chlamydia trachomatis isolates was determined in a tissue culture system. representatives of all currently recognized serotypes of trachoma-inclusion conjunctivitis agents were tested. tetracycline and erythromycin yielded similar results, with 1.0 mug/ml preventing chlamydial replication. rifampin was the most active antibiotic, with 0.25 mug/ml completely suppressing inclusion formation of all strains. fifty percent end points were usually achieved at one-fourt ... | 1977 | 931365 |
| the inactivation of chlamydia trachomatis by povidone-iodine. | | 1976 | 945260 |
| [pathologicoanatomic and histomorphological changes in the fetus in chlamydial abortion in swine]. | | 1976 | 960539 |
| antibodies to two immunotypes of chlamydia trachomatis in individuals with trachoma. | in tunisia (north africa), trachoma remains a common eye disease. most cases are caused by immunotype a of chlamydia trachomatis. in a small proportion of cases (less than 10%), type-specific antibodies to both immunotype a and immunotype b exist in the serum of patients. these types do not crossreact, and occasionally sequential acquisition of antibodies has been demonstrated. thus, individuals in one endemic area of trachoma may be infected with two immunotypes of c. trachomatis. the epidemiol ... | 1976 | 971956 |
| studies on the response of ewes to live chlamydiae adapted to chicken embryos or tissue culture. | ewes infected before gestation with chicken embryo or tissue culture adapted chlamydial strain b-577 were challenge inoculated with the homologous strain at four to 18 weeks of gestation. the ewes responsed with group specific complement fixing antibody titers of 1:8 to 1:256 by the second week after initial infection. a secondary antibody response in the surviving challenge inoculated ewes occurred at the time of lambing and reached titers of 1:32 to 1:256 by the second week after parturition. ... | 1976 | 1000377 |
| isolation of chlamydia trachomatis from women attending a clinic for sexually transmitted diseases. | attempts were made to isolate chlamydia trachomatis from the cervix of 300 women attending a clinic for sexually transmitted diseases in leeds. the women were divided into four groups; (1) 130 were consorts of men suffering from non-specific urethritis; (2) 66 were suffering from gonorrhoea, or were consorts of men suffering from this disease; (3) 56 were suffering from other sexually transmitted diseases; (4) 48 had no evidence of std. the overall isolation rate of chlamydia trachomatis was 20% ... | 1976 | 1009420 |
| antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. i. antigenic heterogeneity between c. trachomatis and c. psittaci. | tw0-dimensional immunoelectrophoresis was used for the antigenic analysis of chlamydia. antigens of chlamydiae were found to be successfully solubilized with the nonionic detergent triton x-100. nineteen and 16 distinct components were observed for chlamydia trachomatis strain l2/434/bu and c. psittaci strain meningopneumonitis/cal-10, respectively. the triton-solubilized antigens of chlamydiae differ strikingly between species since only one strong reciprocal cross-reacting antigen was observed ... | 1975 | 1080772 |
| bacteriological and viral serological observations in egyptian rural school-children with clinical signs of eye infection. | | 1976 | 1088550 |
| topical tetracycline and rifampicin therapy of endemic trachoma in tunisia. | a controlled chemotherapy trial of trachoma was carried out in a tunisian oasis among schoolchildren with active disease. we compared 1% tetracycline ointment (79 patients) or 1% rifampicin ointment (76 patients) with 5% boric acid ointment (79 patients). medications were administered twice daily, six days a week, for ten weeks. slit-lamp examinations by three ophthalmologists were made independently before treatment as well as five, 19, and 39 weeks after treatment. bacteriologic cultures were ... | 1975 | 1096630 |
| minocycline in the treatment of nongonococcal urethritis: its effect on chlamydia trachomatis. | the effect of minocycline on nongonococcal urethritis (ngu) was investigated, with particular reference to its action against chlamydia trachomatis. preliminary laboratory studies showed that the drug was active against a laboratory chlamydial strain (lb4f) in vitro. a group of 133 men with first attacks of ngu was then treated with minocycline, 100 mg twice daily for 3 weeks. isolates of c trachomatis were obtained from 33 of these men before treatment; all of them gave negative results on cell ... | 1975 | 1158770 |
| [use of agar gel precipitation test for the diagnosis of chlamydia infections in the cattle and sheep]. | | 1975 | 1167282 |
| [psittaciadae, bedsonia organisms, fungi and yeasts (author's transl)]. | | 1975 | 1171536 |
| chlamydial eye disease. | in the united states, chlamydial (tric) agents cause a small but important segment of infectious ocular disease. elsewhere in the world, trachoma still represents the single greatest cause of blindness. the description of these diseases in this chapter is a brief introduction to a subject about which volumes have been and will continue to be written. barring unforeseen developments, these agents will continue to plague humankind beyond the end of the twentieth century. | 1975 | 1225860 |
| [isolation of antigen in the diagnosis of enzootic abortion of sheep by use of the complement fixation test]. | | 1975 | 1239846 |
| studies of trachoma in families on taiwan. | this study was undertaken to clarify the natural history and pathogenesis of trachoma. a group of families who live in a formerly trachoma hyperendemic area of southern taiwan were placed under continuous surveillance. the development in recent years of the micro immunofluorescence test for trachoma antibody, along with improved cell culture isolation methods, have allowed this surveillance to include repeated effective laboratory studies in addition to clinical observations. after four years' s ... | 1975 | 1243110 |
| urogenital infection and seminal excretion after inoculation of bulls and rams with chlamydiae. | five mature rams and 4 bulls were inoculated parenterally with bovine or ovine chlamydial strains of type 1 and 2. one to 3 days later, all animals developed a chlamydemia lasting 4 to 8 days. chlamydial agents were isolated from the semen near the end of the chlamydemic phase. all rams and 3 of 4 inoculated bulls excreted chlamydiae in the semen for 22 to 29 days. from 8 to 39 days after inoculation, selected rams or bulls were killed to test for chlamydial infection in the urogenital tract and ... | 1976 | 1275335 |
| severe endemic trachoma in tunisia. | in two villages in southern tunisia where trachoma was endemic 7 per cent and 14 per cent of adults respectively had visual acuity of 20/400 or less. in both villages active trachoma affected most children under the age of two, reached a peak in two- to five-year-olds, then declined to age 15. the chronic inflammatory disease in childhood appeared to produce irreversible scarring of the eyelids, and loss of vision occurred in adult life due to corneal scarring caused by inturned eye lashes and l ... | 1976 | 1276112 |
| convergent and overlapping transcripts of the chlamydia trachomatis 7.5-kb plasmid. | transcription of the 7.5-kb cryptic plasmid of chlamydia trachomatis serovar l2 was investigated. faint, diffuse transcripts of about 1.6 and 2.2 kb and intense short transcripts of about 250 and 430 bases were identified by northern blot analysis. the short transcripts were found to have a common 5' end corresponding to bp 501 relative to the unique bamhi site of the plasmid and to terminate at different downstream sites. putative promoter sequences of ttgcca and tatatt, which closely resemble ... | 1992 | 1281330 |
| binding of chlamydia trachomatis serovar l2 to collagen types i and iv, fibronectin, heparan sulphate, laminin and vitronectin. | chlamydia trachomatis serovar l2 elementary bodies were tested for binding to 125i-labelled components of the extracellular matrix. c. trachomatis bound high levels of collagen type i and heparan sulphate, whereas the binding to collagen type iv, fibronectin, laminin and vitronectin was substantially less. preliminary characterization showed that chlamydial surface proteins are essential for binding to occur. our results suggest that a selective affinity for c. trachomatis to a limited number of ... | 1992 | 1283091 |
| prevalence of urogenital chlamydia trachomatis infection in el salvador. ii. gynaecology outpatients. | the prevalence of urogenital infection caused by chlamydia trachomatis was examined in 100 non-pregnant women with cervicitis, and 100 healthy women, in san salvador city, el salvador. pharmacia chlamydia eia test was used for the detection of chlamydial antigen in urethral and cervical specimens from all the women. direct immunofluorescence was used for confirmative tests on the eia positive and the negative gray zone samples. c. trachomatis antigen was detected in 28% of the women with cervici ... | 1992 | 1286120 |
| [comparison of polymerase chain reaction and ideia chlamydia in detection of chlamydia trachomatis from first-voided urine of male urethritis patients]. | we have reported a method for detection of chlamydia trachomatis by polymerase chain reaction (pcr) with two oligonucleotides based on sequences within the major outer membrane protein gene from c. trachomatis serovar l2. in the previous report, in addition to treatment of the mixture of first-voided urine (fvu) sediment and 1 ml of urine with proteinase k. dna purification by phenol extraction was necessary for preparation of template dna for pcr. in this study, fvu sediment was suspended in 1 ... | 1992 | 1293226 |
| high incidence of asymptomatic urogenital infection in patients with uveitis anterior. | acute anterior uveitis (aau) may be associated with systemic infectious or inflammatory disease. we examined 92 patients with the first attack of acute anterior uveitis; all patients were free of any extraocular symptoms. a thorough clinical examination did not reveal any systemic underlying disease. in the course of microbiological examination, however, a high incidence of asymptomatic infection of the urethra and/or cervix with ureaplasma urealyticum, chlamydia trachomatis and mycoplasma homin ... | 1992 | 1303857 |
| genital papillomavirus infection in women treated for chlamydial infection. | samples for chlamydia testing were taken from 298 and pap smear from 284 non-pregnant sexually active young women in a midwife-run family planning dervice. chlamydia was found in 36 (12.1%). three women (1.1%) had cytological atypia corresponding to cin i or ii. koilocytosis was seen in 9 smears (2.8%). thirty-two of the chlamydia-positive women were followed for a mean of 15 months by a gynaecologist with chlamydia tests, colposcopy, pap-smears and in some cases biopsies. there were 7 reinfecti ... | 1992 | 1311961 |
| [seroepidemiologic study of sexually transmitted diseases caused by chlamydia trachomatis in casablanca (morocco)]. | screening 579 sera specimens obtained in casablanca (morocco) we have performed a retrospective seroepidemiological survey with regard to the prevalence of chlamydia trachomatis involved in genital tract infections. the surveyed populations are divided into 3 groups: 177 patients affected with sexually transmitted diseases (std), 319 reference patients, 83 maghrebian patients affected with std but living in france. the study was performed by the indirect microimmunofluorescence technique. the pr ... | 1992 | 1327348 |
| prevalence and cytologic manifestations of human papilloma virus (hpv) types 6, 11, 16, 18, 31, 33, 35, 42, 43, 44, 45, 51, 52, and 56 among 500 consecutive women. | the prevalence and associated cytologic manifestations of cervical infection with human papillomavirus (hpv) types 6, 11, 16, 18, 31, 33, 35, 42, 43, 44, 45, 51, 52, and 56 were studied among 500 consecutive women attending the harborview medical center sexually transmitted diseases (std) clinic in seattle, wa. using radiolabeled-probes without prior amplification of dna, hpv dna was detected in cervical specimens from 120 (24%) of the women and was found to be more prevalent than chlamydia trac ... | 1992 | 1328077 |
| a cohort study of the risk of cervical intraepithelial neoplasia grade 2 or 3 in relation to papillomavirus infection. | human papillomavirus (hpv) has been associated with cervical intraepithelial neoplasia, but the temporal relation between the infection and the neoplasia remains unclear, as does the relative importance of the specific type of hpv, other sexually transmitted diseases, and other risk factors. | 1992 | 1328880 |
| sexually transmitted diseases: perspectives on this growing epidemic. | despite advances with antibiotic therapies and increased public attention to acquired immunodeficiency syndrome, sexually transmitted diseases remain a major public health problem of epidemic proportions in the united states. the emergence of new pathogens and clinical syndromes has increased the complexity of the situation. incurable conditions and asymptomatic disease states during an era of constrained public resources and deficient public awareness further heighten and compound the devastati ... | 1992 | 1328969 |
| azithromycin: the first azalide antibiotic. | to discuss the chemistry, mechanism of action, spectrum of activity, pharmacokinetics, clinical trials, adverse-effect profile, drug interactions, and dosage guidelines of azithromycin, the first azalide antibiotic. | 1992 | 1330097 |
| [prevalence of chlamydia trachomatis in genital samples from abidjan]. | a study of direct genital swabs achieved in abidjan, on 116 men and 131 women consulting for urogenital complaints, has revealed that the men show a prevalence of 28.4% chlamydia trachomatis, and of 18.1% neisseria gonorrhoeae. concerning the women the prevalence of the same germs are 13.7% for chlamydia trachomatis, and 4.6% for neisseria gonorrhoeae. these results show the importance of chlamydia trachomatis as a sexually transmitted disease in abidjan (côte-d'ivoire). no differences were obse ... | 1992 | 1330137 |
| abc of colorectal diseases. sexually transmitted diseases and anal papillomas. | | 1992 | 1330142 |
| efficacy of single-dose azithromycin versus doxycycline in the treatment of cervical infections caused by chlamydia trachomatis. | the efficacy of single-dose azithromycin therapy in the treatment of cervical chlamydia trachomatis infections was compared to that of a standard seven-day course of treatment with doxycycline. cervical samples from 60 patients reacted positively in an enzyme immunoassay for detection of chlamydia trachomatis. in 31 patients chlamydia trachomatis was isolated from the sample taken before treatment. fourteen of the 31 patients were treated with doxycycline and 17 with azithromycin. all cultures o ... | 1992 | 1330565 |
| reversibility of heat shock in chlamydia trachomatis. | the heat shock effect on chlamydia development was studied. we report here that the reversibility of the heat shock response did not depend on the stage of chlamydial morphogenesis at which transfer to high temperature occurred, and the infectivity of the particles produced was not affected significantly, so long as the heat shock exposure was not prolonged. exposure to heat shock for more than 9 h resulted in stagnation of the growth cycle, appearance of aberrant reticulate body particles and l ... | 1992 | 1330821 |
| a double blind study of single dose azithromycin and doxycycline in the treatment of chlamydial urethritis in males. | to compare the efficacy and safety of azithromycin and doxycycline in the treatment of males with uncomplicated urethritis caused by chlamydia trachomatis. | 1992 | 1330880 |