| fatal septicaemia caused by vibrio vulnificus. | | 1988 | 3399921 |
| vibrio vulnificus septicemia. | we report a case of vibrio vulnificus infection in a middle-aged alcoholic man with laennec's cirrhosis. the patient had recently received a puncture wound from the shell of a shrimp while fishing in the gulf of mexico. he presented with acrally distributed urticarial plaques, purpura, and bullae, as well as signs and symptoms of septic shock. vibrio vulnificus was isolated from the blood, and histologic examination of the skin biopsy specimen demonstrated a devitalized, inflammatory, cell-poor ... | 1988 | 3401105 |
| spinal cord penetration of intrathecally administered cytarabine and methotrexate: a quantitative autoradiographic study. | carcinomatous meningitis is an increasingly common complication of systemic cancer. treatment usually involves the use of intrathecal methotrexate or cytarabine, which have been reported to be toxic to the spinal cord. this study was performed to determine the penetration and distribution of intrathecally administered chemotherapy within the spinal cord. tritiated methotrexate and cytarabine were administered intraventricularly and by lumbar puncture to new zealand white rabbits. the extent and ... | 1988 | 3418727 |
| common genetic pools between human populations. | a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ... | 1988 | 3422217 |
| effect of pre- and postnatal exposure to alcohol on perivenous and periportal neonatal rat hepatocytes. | hepatocytes from 12-day-old rats, pre and postnatally exposed to alcohol and pair-fed controls, were isolated and subfractionated in six cell subpopulations on percoll density gradients. these cells were characterized using biochemical, stereological and cytofluorometric methods. our results show that the low density cells (f2) are mainly perivenous cells, whereas the heavier cells (f6) were primarily periportal cells. these results were confirmed by alanine aminotransferase (alat), and glutamat ... | 1987 | 3426691 |
| vibrio vulnificus infections in singapore. | | 1987 | 3429797 |
| liver cell lines. | | 1987 | 3431441 |
| [hypoxia and protection of the brain (isoflurane vs halothane and enflurane]. | | 1987 | 3438012 |
| destructive chronic borrelia meningoencephalitis in a child untreated for 15 years. | a case of borrelia spirochetosis with recurring episodes of progressively destructive chronic meningoencephalitis, untreated for 15 years, is presented. the patient, now 23 years old, represents an example of the natural course of the disease and may serve as a reminder of the diagnosis in retrospective cases as well as prospectively for acute and chronic states of serous meningitis/meningoencephalitis both in children and adults. | 1987 | 3441752 |
| monoclonal antibodies against the haemolysin of vibrio vulnificus. | the extracellular haemolysin produced by vibrio vulnificus strain fcc was partially purified from the culture supernate by sequential ammonium sulphate precipitation, gel filtration with sepharose 4b, and deae-sephacel ion-exchange column chromatography. using this semi-purified haemolysin as the antigen, several monoclonal antibodies (mabs) were established; they were all of the igg2b class with lambda light chains. one representative mab, 6f8d, completely neutralized the haemolytic activity an ... | 1987 | 3443854 |
| the role of catecholamines and glucagon on serum and liver metallothionein response to restraint stress. | the role of glucagon and catecholamines on serum and liver metallothionein (mt) concentrations in basal and stress conditions has been studied. glucagon showed no effect on serum mt either in basal (unstressed) or stress (20 hours of restraint) conditions. in contrast, glucagon administration increased both unstressed and stressed levels of liver mt. no effect of the alpha + beta-catecholamine blocker labetalol on serum mt levels was observed in unstressed rats. however, the administration of la ... | 1987 | 3448709 |
| variability of haemolysin(s) produced by vibrio vulnificus. | the peptide composition and antigenic cross-reactivity of partially purified and concentrated haemolysins of 16 strains of vibrio vulnificus were examined by sds-page and immunoblotting analysis, using a monoclonal antibody (mab), 6f8d, raised against the haemolysin. all strains produced a common peptide of 36 kda and the mab reacted with this peptide. in some strains, larger molecules, including a 56 kda peptide, were produced, but the mab did not react with this peptide. the haemolytic activit ... | 1987 | 3449599 |
| mechanism of haemolysis by vibrio vulnificus haemolysin. | the haemolytic action of vibrio vulnificus haemolysin (vvh) was compared to that of streptolysin o (slo). both were cholesterol-binding haemolysins, but differed in the release of haemoglobin (hb). in the first step of haemolysis, the haemolysins were temperature-independently bound to the cholesterol site on the target erythrocyte membrane. this was followed by the rapid release of k+, which is an intra-erythrocyte marker. hb was then released, in different ways. in the case of vvh, hb was rele ... | 1987 | 3449600 |
| [human gingival pigmentation due to electrochemical degradation. immunological study of biopsies using monoclonal antibodies]. | | 1986 | 3464603 |
| [study on prostaglandin e involved in tooth movement--immunohistochemical observation]. | | 1987 | 3475379 |
| comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization. | the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ... | 1987 | 3478194 |
| [mast cell-affinity lymphocytes as indicator cells of the forms of hemoblastoses]. | the formation of mastolymphocyte rosettes was used to determine that in chronic lymphocytic leukemia and bovine leukemia, unlike healthy people and animals as well as patients with solid tumours of different localization and hodgkin's disease, a large amount of cells appear among peripheral blood lymphocytes having membrane affinity for mast cells. this may be used as an additional criterion in laboratory diagnosis of these diseases. | 1986 | 3485042 |
| experience with isoxicam and catabolin. | 1 synovial cells produce a protein factor, catabolin, that induces chondrocytes to resorb their own matrix with the release of glycosaminoglycan (gag). 2 studies were performed to determine the effect of isoxicam, a new nsaid, on gag production in a catabolin system utilizing tissue culture of bovine nasal septum in a medium containing pooled synovial tissue from young pigs. 3 using fresh medium containing no synovial tissue, the release of gag by inherent cartilage breakdown was 49.7% after 8 d ... | 1986 | 3497659 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro. | osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ... | 1987 | 3502681 |
| tissue-specific and developmental expression of human transthyretin gene in transgenic mice. | to analyze the regulation of transthyretin gene expression we have produced transgenic mice by microinjecting cloned human transthyretin genes into fertilized eggs of c57bl/6 mice. the 7.6-kilobase (kb) human transthyretin gene containing about 500 base pairs (bp) in the upstream region was used for microinjection. seven out of nine transgenic mice had detectable amounts of human transthyretin in serum when analyzed by enzyme-linked immunosorbent assay. transthyretin mrna was detected in liver a ... | 1987 | 3502973 |
| a quantitative morphological examination of bovine vulval skin glands. | in adult cows the volumes of sweat and sebaceous glands per unit skin surface area (ssa) in the vulva were greater (p less than 0.01) than in the neck, lip or eyelids. within this perineal region skin gland volumes were greater dorsally than ventrally (p less than 0.01). adult cows had much greater (p less than 0.01) volumes of perineal skin glands per unit ssa than 8 weeks or 6 weeks old heifer calves or steers. there was little or no difference between animal categories in neck skin gland dime ... | 1987 | 3503046 |
| rhamnetin-3-o-neohesperidoside, a new flavonoid from the leaves of derris trifoliata. | | 1986 | 3509960 |
| potentiation of hemorrhage-evoked catecholamine release by prior blood loss in cats. | the effect of prior blood loss on the plasma catecholamine response to acute hemorrhage (h) was assessed in alpha-chloralose-urethane anesthetized cats. animals sustained an initial h period of 0 (samples only), 10, or 20% h total blood volume. ninety minutes after reinfusion of the shed blood, all animals sustained a rapid 20% h. the catecholamine and arterial pressure responses to this second 20% h were assessed every 2 min for 20-min duration. plasma norepinephrine increased modestly in the 0 ... | 1986 | 3510559 |
| escherichia coli as cause of ecthyma gangrenosum. | | 1986 | 3511469 |
| feasibility of same-day identification of members of the family vibrionaceae by the api 20e system. | sixty isolates, comprising nine species of the family vibrionaceae, were tested with the api 20e 5-h same-day procedure (analytab products, plainview, n.y.). included were 27 aeromonas hydrophila isolates, 10 aeromonas sobria isolates, 7 aeromonas caviae isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolate. the 5-h profile numbers were specific fo ... | 1986 | 3517061 |
| immunocytochemical localization of cathepsin b in rat kidney. ii. electron microscopic study using the protein a-gold technique. | thin sections of lowicryl k4m-embedded materials were labeled with protein a-gold complex. gold particles representing the antigen sites for cathepsin b were exclusively confined to lysosomes of each segment of the nephron. the heaviest labeling was noted in the lysosomes of the s1 segment of the proximal tubules. labeling intensity varied considerably with the individual lysosomes. lysosomes of the other tubular segments, such as the s2 and s3 segments of the proximal tubules, distal convoluted ... | 1986 | 3519753 |
| production of extracellular enzymes and cytotoxicity by vibrio vulnificus. | thirty-three strains of vibrio vulnificus of clinical and environmental origin were examined for production of 12 extracellular enzymes of potential importance to the virulence of this bacterium. strains of vibrio vulnificus were consistent in their production of protease, mucinase, lipase, chondroitinase, hyaluronidase, dnase, sulfatase, and hemolysin. no differences between clinical and environmental isolates were noted. although none of the enzymes appeared to correlate with the ability of th ... | 1986 | 3522090 |
| chitinase determinants of vibrio vulnificus: gene cloning and applications of a chitinase probe. | to initiate study of the genetic control of chitinolytic activity in vibrios, the chitobiase gene was isolated by cloning chromosomal dna prepared from vibrio vulnificus. chimeric plasmids were constructed from sau3a i partial digests of chromosomal dna by ligating 5 to 15-kilobase fragments into the bamhi site, i.e., in the tcr gene, of pbr322 (amr tcr). the resulting plasmids were transformed into escherichia coli dh1. chitobiase activity of the insert-bearing clones was detected by using a ch ... | 1986 | 3524459 |
| hemorrhagic bullae associated with vibrio vulnificus septicemia. report of two cases. | bullous lesions associated with vibrio vulnificus infection developed in two patients, both of whom had hepatic cirrhosis. one patient had a recent history of ingestion of raw oysters, while the other patient had recently exposed skin lacerations to sea water. both patients died within 24 hours of hospitalization, in spite of antibiotic treatment. vibrio vulnificus was isolated from blood and bullae in both patients. histologic examination of skin biopsy specimens revealed epidermal/dermal separ ... | 1986 | 3524472 |
| phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells. | we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ... | 1987 | 3543022 |
| phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells. | we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ... | 1987 | 3543022 |
| the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei. | fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ... | 1987 | 3554006 |
| calcitonin gene-related peptide in the ventral tegmental area: selective modulation of prefrontal cortical dopamine metabolism. | the distribution of calcitonin gene-related peptide (cgrp) in the a10 dopamine (da) cell group region of the ventral tegmental area (vta) of the rat was examined using immunohistochemical techniques. cgrp-like immunoreactivity was localized to axons innervating the rostral and dorsal vta. direct administration of cgrp to the vta of the rat resulted in a dose-related increase in da utilization in the medial prefrontal cortex, but not other mesocortical, mesolimbic, or striatal da terminal field r ... | 1987 | 3554009 |
| [a case of sepsis, shock, disseminated intravascular coagulation, and necrotizing fasciitis caused by vibrio vulnificus treated with early debridement]. | | 1986 | 3559356 |
| ranking possible carcinogenic hazards. | this review discusses reasons why animal cancer tests cannot be used to predict absolute human risks. such tests, however, may be used to indicate that some chemicals might be of greater concern than others. possible hazards to humans from a variety of rodent carcinogens are ranked by an index that relates the potency of each carcinogen in rodents to the exposure in humans. this ranking suggests that carcinogenic hazards from current levels of pesticide residues or water pollution are likely to ... | 1987 | 3563506 |
| vibrio vulnificus septicemia: report of four cases. | | 1986 | 3564544 |
| variation in the lymphocyte transformation assay. slope analysis of cell dose-response curves. | blood samples from a single sheep were cultured at a number of different cell concentrations and stimulated with three lymphocyte mitogens, phytohaemagglutinin, concanavalin a and pokeweed mitogen. the response to stimulation, measured as log thymidine uptake (log cpm) was found to be a linear function of log cell concentration (log cell dose). samples taken on different dates, varied significantly in the gradients of the log cpm: log cell dose plots. these alterations of gradient constituted th ... | 1987 | 3571985 |
| aids and non-human primates. | | 1987 | 3576926 |
| radiosensitivity and sublethal damage repair in human umbilical cord vein endothelial cells. | the assessment of the comparative radiosensitivity of different parenchymal cells is potentially of great importance in clinical radiotherapy. previous studies from various laboratories using vessel regeneration techniques have demonstrated that endothelial cells are quite radioresistant, having a do in excess of 250 rads. the present studies were undertaken to investigate the radiosensitivity of human endothelial cells obtained from umbilical cord veins (hec) using "colony formation" as an endp ... | 1987 | 3583859 |
| testicular defect: the primary abnormality in gonadal dysfunction of uremia. | gonadal dysfunction is a common problem in end-stage renal disease. we decided to test the ability of the male gonads to respond to exogenous stimuli. the patients receiving chronic hemodialysis showed abnormally low levels of total testosterone and free testosterone in the presence of normal testosterone-binding globulin. furthermore, the exogenous administration of human chorionic gonadotropin failed to elicit a normal response from the male gonads of hemodialyzed patients. although basal seru ... | 1987 | 3589761 |
| noninvasive doppler assessment of human postprandial renal blood flow and cardiac output. | we compared the effects of protein, carbohydrate, or water ingestion on human renal blood flow (rbf) and cardiac output (co). we validated and applied a noninvasive ultrasonic doppler method to measure blood velocities and lumen diameters in the right renal artery and the ascending aorta of five healthy adults. from these measurements, we calculated the average rbf and co over five cardiac cycles. normalizing to body surface area, renal blood flow index to one kidney (ri) and cardiac index (ci) ... | 1987 | 3591956 |
| storage conditions and stability of thyrotropin and thyroid hormones on filter paper. | we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ... | 1987 | 3594835 |
| storage conditions and stability of thyrotropin and thyroid hormones on filter paper. | we evaluated the effects of temperature and humidity on thyroid hormones (t4, t3) and thyrotropin (tsh) measured in blood spots dried on filter paper. rias for t4 and t3 blood spots were optimized to measure these analytes over the neonatal and euthyroid adult reference intervals. sensitivities of the t3 and t4 assays were 0.5 and 10 nmol/l, respectively. a blood-spot immunoradiometric assay for tsh involving magnetizable particles was developed with a sensitivity of 6 milli-int. units/l. contro ... | 1987 | 3594835 |
| fibre types in extraocular muscles: a new myosin isoform in the fast fibres. | we report on the existence of a myosin heavy chain (mhc) isoform with unique structural properties in extraocular (eo) muscles. differences in mhc composition are apparent using a polyclonal antibody prepared against myosin isolated from bovine eo muscle myosin. in enzyme immunoassays and western blotting experiments, this anti-eo myosin antibody reacted specifically with the heavy chains of eo muscle myosin and not with the heavy chains of other myosins. the distribution of this new mhc isoform ... | 1987 | 3597767 |
| fibre types in extraocular muscles: a new myosin isoform in the fast fibres. | we report on the existence of a myosin heavy chain (mhc) isoform with unique structural properties in extraocular (eo) muscles. differences in mhc composition are apparent using a polyclonal antibody prepared against myosin isolated from bovine eo muscle myosin. in enzyme immunoassays and western blotting experiments, this anti-eo myosin antibody reacted specifically with the heavy chains of eo muscle myosin and not with the heavy chains of other myosins. the distribution of this new mhc isoform ... | 1987 | 3597767 |
| thin collagenous septa in cardiac muscle. | light and electron microscopy were used to study the structure and distribution of thin collagenous septa (sheets) in dog and rabbit cardiac muscle to determine whether they, like thick collagenous septa, could affect electrical impulse propagation. generally, thin septa (0.2-0.5 micron) ensheathed myocytes or groups of myocytes for short distances and thicker septa partially or completely ensheathed groups of myocytes for long distances (up to several mm); together, thin, and thick septa divide ... | 1987 | 3605660 |
| virulent strains of vibrio vulnificus isolated from estuaries of the united states west coast. | vibrio vulnificus was isolated from united states west coast estuaries at a low frequency (5.9%) from 529 samples of water, shellfish, and sediment. four strains tested with iron-treated mice had 50% lethal dose values ranging from 7.6 to 360 cfu, compared with a 50% lethal dose of 4.9 cfu for a clinical isolate that caused the death of a septicemic patient. the presence of this pathogen may be a hazard to users of marine beaches and consumers of raw shellfish on the west coast, especially to pe ... | 1987 | 3606112 |
| plasmid-mediated trimethoprim-resistance in staphylococcus aureus. characterization of the first gram-positive plasmid dihydrofolate reductase (type s1). | the trimethoprim-resistance gene located on plasmid psk1, originally identified in a multi-resistant staphylococcus aureus from australia, encodes the production of a dihydrofolate reductase (type s1), which confers a high degree of resistance to its host and is quite unlike any plasmid-encoded dihydrofolate reductase hitherto described. it has a low mr (19,700) and has a higher specific activity than the constitutive gram-negative plasmid dihydrofolate reductases. the type s1 enzyme is heat-sta ... | 1987 | 3606580 |
| [analysis of lethal toxin produced by vibrio vulnificus]. | | 1987 | 3610014 |
| differing resistance to polymorphonuclear cells of halophilic- and hypotonic-types of vibrio vulnificus. | bacterial growth and lethality of 4 strains of vibrio vulnificus infection of mice were enhanced by gamma-irradiation but not by treatment with carrageenan. therefore, protection against v. vulnificus, at least in the early phases, probably depends mainly on polymorphonuclear cells (pmn), since carrageenan depletes macrophages but not pmn. pmn dependent protection against infection differs for 2 types of v. vulnificus strains. halophilic- and hypotonic-type were distinguished from the correspond ... | 1987 | 3612754 |
| intrinsic circuitry involving the local axon collaterals of corticothalamic projection cells in mouse smi cortex. | the objective of this study was to identify the components involved in a local synaptic circuit in the mouse cerebral cortex. the local axon collaterals of corticothalamic (ct) projection cells in the posteromedial barrel subfield area of primary somatosensory cortex were labeled by the retrograde transport of horseradish peroxidase injected into the ipsilateral thalamus. thalamocortical (tc) axon terminals in the same region of cortex were labeled by lesion induced degeneration. ct axon termina ... | 1987 | 3624546 |
| prenatal diagnosis of minute 5p- deletion: a cytogenetic problem in detection. | an unbalanced translocation, 46,xx,der(5)t(5;11) (p15;q25)mat was ascertained by prenatal diagnosis. the mother previously had a liveborn child with cri du chat syndrome. the subtlety of the chromosome rearrangement in this case illustrates the need for the most detailed cytogenetic investigations in cri du chat cases when deletion or translocation are not immediately obvious. | 1987 | 3627600 |
| activation of the plasma kallikrein-kinin system by vibrio vulnificus protease. | vibrio vulnificus protease enhanced hypodermic vascular permeability when injected into the dorsal skin of a guinea pig. enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at about 10 min postinjection. the permeability-enhancing reaction was greatly augmented by simultaneous injection of a kininase ii inhibitor, whereas the reaction was inhibited by soybean trypsin inhibitor, a well-known inhibitor of plasma kallikrein. furthermore, in vitr ... | 1987 | 3649335 |
| nonradioactive, photobiotin-labelled dna probes for the routine diagnosis of barley yellow dwarf virus. | photobiotin was used to prepare biotinylated, nonradioactive nucleic acid probes for the detection of the rna of barley yellow dwarf virus (bydv) in plant extracts. a 1.7-kb cdna of the pav isolate of bydv in the plasmid puc8 vector was biotinylated and then used intact or as sonicated double-stranded dna fragments. simple methods were developed for the preparation of partially purified nucleic acid extracts of cereals and their spotting, after formaldehyde treatment, onto nitrocellulose membran ... | 1987 | 3654900 |
| use of sodium dodecyl sulfate-polymyxin b-sucrose medium for isolation of vibrio vulnificus from shellfish. | the differential and selective sodium dodecyl sulfate-polymyxin b-sucrose medium (sps) of kitaura et al. (t. kitaura, s. doke, i. azuma, m. imaida, k. miyano, k. harada, and e. yabuuchii, fems microbiol. lett. 17:205-209, 1983), which highlights alkylsulfatase activity, was evaluated for its potential use in the direct isolation and enumeration of vibrio vulnificus from shellfish. v. vulnificus was detected by this method in six of nine shellfish samples collected from diverse geographic locales ... | 1987 | 3662506 |
| na+ modulates the k+ permeability and the membrane potential of alkalophilic bacillus. | in the absence of na+ in the medium, the membrane potential of obligately alkalophilic bacillus cells was found to be decreased by the addition of k+ to the medium, whereas k+ addition in the presence of na+ had no effect. rb+ showed essentially the same effect as k+. the decreased membrane potential was quickly restored by lowering the k+ concentration in the medium or by adding na+ or li+ to the medium. thus, in the absence of na+, the membrane potential of alkalophilic bacillus seems to be af ... | 1987 | 3663674 |
| vibrio vulnificus. | v vulnificus is a halophilic or salt-requiring vibrio that has been isolated repeatedly from seawater and shellfish in coastal waters. this vibrio, first described by hollis et al in 1976, can be differentiated from other similar vibrios by its ability to ferment lactose and by its lower tolerance for sodium chloride. v vulnificus, unlike most other vibrios, has seldom been incriminated as a cause of gastroenteritis but is a particularly virulent organism that causes severe wound infections in m ... | 1987 | 3667122 |
| new selective and differential medium for vibrio cholerae and vibrio vulnificus. | thiosulfate-citrate-bile salts-sucrose agar has been routinely used for the isolation of pathogenic vibrios, although its selectivity for vibrio cholerae and vibrio vulnificus is inadequate. therefore, a new plating medium, cellobiose-polymyxin b-colistin agar, was developed for the isolation of these two species. cellobiose-polymyxin b-colistin agar demonstrated a significant advantage over other media designed for the isolation or differentiation of vibrios: of both the 136 strains representin ... | 1987 | 3674873 |
| vibrio vulnificus bacteremia with endocarditis. | | 1987 | 3686154 |
| distribution of immunoreactive inhibin like material in different regions of human prostate. | distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05). | 1987 | 3688474 |
| analysis of protein patterns from different organs and cell fractions of trisomy 19 mice. | proteins were extracted from liver, brain, and skin of 6-day-old mice with trisomy (ts) 19 and fractionated into solubilized cell proteins and structure-bound cell proteins. the proteins were separated by two-dimensional electrophoresis, and protein patterns were compared in the combinations ts/normal and normal/normal. analysis of the protein patterns revealed protein spots (variants) with densities higher (h-type) or lower (l-type) in trisomies than in normal mice. some of these variants were ... | 1987 | 3692480 |
| effect of iron and liver injury on the pathogenesis of vibrio vulnificus. | noncholera vibrio species can cause necrotizing soft tissue infections and cellulitis in addition to gastroenteritis. cellulitis and necrotizing soft tissue infections frequently occur in people with liver disease or in people who are in some way a compromised host. we studied the effect of vibrio vulnificus on the mortality of mice treated with carbon tetrachloride, an agent that causes liver damage, desferal, an iron-chelating agent, or iron. the ld50 of v. vulnificus is approximately 2.5 x 10 ... | 1987 | 3695454 |
| composition of major outer membrane proteins of vibrio vulnificus isolates: effect of different growth media and iron deficiency. | the outer membrane proteins of vibrio vulnificus including isolates from humans, seawater and an asari clam were examined by sodium dodecyl sulfate (sds)-polyacrylamide gel electrophoresis. a major outer membrane protein with an apparent molecular weight of 48,000 (48k protein) was common to all the strains grown in 3% nacl-nutrient broth; however this 48k protein was not produced in any of the strains grown in chemically defined medium. other major outer membrane proteins with molecular weights ... | 1986 | 3724558 |
| virulence of vibrio vulnificus strains from marine environments. | vibrio vulnificus strains isolated from geographically diverse marine sources were compared with clinical isolates for phenotype and in vitro and in vivo production of virulence factors. there were no differences between environmental and clinical strains on the basis of biochemical characteristics or antimicrobial susceptibility patterns. cytolysin and cytotoxin titers produced by environmental strains were generally comparable to those of clinical strains. of 29 environmental isolates tested, ... | 1986 | 3729387 |
| plasmid carriage in vibrio vulnificus and other lactose-fermenting marine vibrios. | a total of 42 clinical and environmental isolates of vibrio vulnificus were examined for plasmid carriage. of these, only five (12%) harbored plasmids, which were of various molecular weights. in contrast, 20 of 32 (62.5%) unidentified lactose-fermenting vibrio spp. were found to possess plasmids with masses of 2.1 to 150 megadaltons. in these isolates, multiple plasmids were common, with an average of 2.25 plasmids per plasmid-containing strain. attempts to demonstrate a correlation with the pl ... | 1986 | 3729404 |
| purification and initial characterization of the 71-kilodalton rat heat-shock protein and its cognate as fatty acid binding proteins. | the major rat heat-shock (stress) protein and its cognate were purified to electrophoretic homogeneity from livers of heat-shocked rats. both proteins exhibited similar behavior on a variety of column chromatography matrices but were separable by preparative isoelectric focusing under nondenaturing conditions by virtue of a 0.2 ph unit difference in isoelectric point. both purified proteins had similar physical properties, suggesting the possibility that they may have similar biological function ... | 1986 | 3730359 |
| preparation, isolation and characterization of mouse ige monoclonal antibodies against trichosanthin protein. | | 1986 | 3739501 |
| a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle. | serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ... | 1986 | 3753338 |
| calmodulin gene expression during sea urchin development: persistence of a prevalent maternal protein. | calmodulin gene expression during embryogenesis of the sea urchin strongylocentrotus purpuratus was investigated. several identical bacteriophages containing a cdna insert encoding sea urchin calmodulin (cm-1) were identified by screening a lambda gt10 library of s. purpuratus gastrula-stage cdnas with a chicken calmodulin cdna sequence. a 1.2-kb cdna fragment from cm-1 was subcloned into puc-8 to give plasmid pcal-8. pcal-8 contains a single open reading frame encoding 79 amino acids, a termina ... | 1986 | 3753945 |
| studies on cardiac distribution and function of neuropeptide y. | high concentrations of a novel peptide, neuropeptide y, have been demonstrated in the guinea-pig and canine heart and in the latter, a particularly high concentration was found in the region of the coronary vasculature (126 +/- 31 pmol g-1). intra-arterial infusion of neuropeptide y for 30 s into the coronary artery of the intact, innervated dog heart resulted in a rapid and short-lasting reduction of blood flow from 38 +/- 4 to 31 +/- 3 ml min-1 (p less than 0.05) to resume control level, 39 +/ ... | 1986 | 3754377 |
| effect of alterations in lipid packing order by hydrophobic solutes on the association state of protein assemblies in model membranes. | the effects of two hydrophobic solutes which perturb lipid packing order, permethrin and allethrin, on the aggregated state of a lipid membrane-incorporated protein, bacteriorhodopsin (br), have been determined by resonance energy transfer measurements. as temperature is increased from well below the main gel-fluid phase transition temperature (tc) of the lipid, patches of aggregated br dissociate into monomers, a few degrees below the tc (m.p. heyn, a. blume, m. rehorek and n.a. dencher, bioche ... | 1986 | 3754778 |
| stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation. | stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ... | 1986 | 3756629 |
| breath hydrogen concentration and small intestinal malabsorption in calves. | breath hydrogen concentrations were measured to assess intestinal carbohydrate malabsorption in preruminating calves. oral administration of 1.25 g of lactulose (a nonabsorbable carbohydrate)/kg to calves produced breath hydrogen concentrations significantly (p less than 0.001) higher than values determined after calves were fed milk and before the treatment was given. this indicates that, in the calf, fermentation of nonabsorbed carbohydrates results in increased breath hydrogen values. to indu ... | 1986 | 3767107 |
| sensitive analytical method for serum primidone and its active metabolites for single-dose pharmacokinetic analysis in human subjects. | | 1986 | 3769107 |
| participation of iron on the growth inhibition of pathogenic strains of mycobacterium avium and m. paratuberculosis in serum. | serum, which is low in freely-available iron, was bacteriostatic for m. avium and m. paratuberculosis irrespective of the ability of these organisms to produce the iron-chelators mycobactin and exochelin for iron acquisition. bacteriostasis was overcome when the serum was supplemented with exochelin or mycobactin, but only if the mycobacteria had previously adapted to growth in a low iron environment. the results imply that for studies on mycobacterial pathogenicity the test cultures should be g ... | 1986 | 3788345 |
| isolation and characterization of a mucin-degrading strain of peptostreptococcus from rat intestinal tract. | a mucin-degrading microorganism was isolated from the intestinal tract by serial sectioning from the serosal side of the caecum wall from a conventional rat. the ability of degrading the intestinal water-soluble mucin was present both in vivo after monocontamination of germ-free rats and in vitro, when adding the microbe to mucin medium. the morphology, gram-positive cocci single or in short chains and the very weak biochemical activities allow us to place this strain in the species peptostrepto ... | 1986 | 3788570 |
| bioluminescence in a strain of the human pathogenic bacterium vibrio vulnificus. | we report the existence of a bioluminescent strain of the human pathogen vibrio vulnificus. the isolate was obtained from blood following a fatal wound infection and thus represents the first description of an infection caused by a luminescent bacterium. | 1986 | 3789716 |
| rapid serological identification of vibrio vulnificus by anti-h coagglutination. | staphylococcus aureus cowan 1 cells were armed with anti-flagellar (anti-h) antibody produced in rabbits immunized with flagellar core protein prepared from vibrio vulnificus. this reagent was assessed by coagglutination for its capacity to agglutinate and identify v. vulnificus. a species-specific h antigen is expressed in the core proteins of the polar flagella of v. vulnificus. of 435 v. vulnificus isolates identified bacteriologically, 432 (99.3%) were agglutinated in the slide test within 2 ... | 1986 | 3789720 |
| comparison of histones in normal and xeroderma pigmentosum lymphoblastoid cells. | histones from normal human and xeroderma pigmentosum, complementation group a (xpa), lymphoblastoid cells were compared both quantitatively, qualitatively and for binding affinity for dna. electrophoretic examination of the histones showed that all five major histone species were present in both cell groups and that there were no quantitative differences between normal and xpa histones. binding affinity to [3h] mammalian dna of the histones was determined. no significant differences were observe ... | 1986 | 3791423 |
| surgical management of peritoneal dialysis catheters in children: five-year experience with 1,800 patient-month follow-up. | currently at our institution more than 90% of the children with end-stage renal disease are managed with continuous ambulatory peritoneal dialysis (capd) in preference to hemodialysis until a successful transplant is accomplished. recent refinements in capd catheters and dialysis techniques have greatly added to the many medical, psychological, and economic advantages of capd compared with chronic hemodialysis. ninety-three patients less than 21 years of age underwent insertion of 167 peritoneal ... | 1986 | 3794985 |
| [a case of vibrio vulnificus infection]. | | 1986 | 3795561 |
| [changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct]. | competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease. | 1986 | 3801148 |
| mouse skin damage caused by cytolysin from vibrio vulnificus and by v. vulnificus infection. | light and electron microscopy of mouse skin damage caused by intradermal infection with a virulent strain of vibrio vulnificus and by a single intradermal injection of the cytolytic toxin produced by the bacterium revealed similar structural alterations. the epidermis was intact; however, the infection and toxin produced acute cellulitis characterized by extensive extracellular edema; disorganization of collagen bundles; large accumulations of cell debris and plasma proteins; damaged or necrotic ... | 1987 | 3805762 |
| pneumonia--the quality of medical records data. | the quality of medical records data for patients who were hospitalized with community-acquired pneumonia was assessed by comparing medical records data with data obtained in a prospective study of pneumonia for the period april 1, 1984, to december 31, 1984. one hundred five patients fulfilled the case definition of pneumonia for entry into the prospective study. one hundred twenty-seven patients were identified by medical records data. seventy-three of the patients appeared in both studies. the ... | 1987 | 3807447 |
| comparison of the pharmacological properties of pituitary and biosynthetic human growth hormone. demonstration of antinatriuretic/antidiuretic and barbital sleep effects of human growth hormone in rats. | biosynthetic human growth hormone was compared with pituitary human growth hormone and pituitary 22 k in the weight gain and the tibia test. the three preparations were found to be equipotent. furthermore, the growth hormones were compared in various pharmacological test systems. all three preparations were found to have a marked antidiuretic and antinatriuretic effect in the rat and to cause a significant shortening of the hexobarbital sleeping time in mice. biosynthetic and pituitary preparati ... | 1987 | 3811769 |
| evaluation of the hereditary syrian hamster cardiomyopathy by 31p nuclear magnetic resonance spectroscopy: improvement after acute verapamil therapy. | the relation between metabolic and functional derangement in various cardiomyopathies has not been well characterized. this information was specifically sought in a spontaneous cardiomyopathic model. metabolic and hemodynamic parameters were obtained in glucose-perfused beating hearts of 180-200-day-old cardiomyopathic syrian hamsters and age-matched healthy animals. this period in the cardiomyopathic hamster lifetime is intermediary between the necrotic phase and the appearance of heart failure ... | 1986 | 3815756 |
| clinical and ecological characteristics of vibrio vulnificus in the northeastern united states. | multiple seawater sites in the northeastern united states, particularly long island sound, and shellfish from long island sound were sampled from april to november for 3 successive yr, 1983-1985. hospitals in coastal and metropolitan areas of connecticut were surveyed for the same 3-yr period, vibrio vulnificus can be found in these waters during the summer months. the appearance of these virulent bacteria in both seawater and shellfish are a function of the water temperature; no v. vulnificus c ... | 1987 | 3816129 |
| vibrio vulnificus infection after raw oyster ingestion in a patient with liver disease and acquired immune deficiency syndrome-related complex. | sepsis, peritonitis, and gastroenteritis developed in a 45-yr-old homosexual man 1 day after ingestion of raw oysters. the patient had chronic active hepatitis and cirrhosis with hepatitis b virus and delta-infection. he also had persistent generalized lymphadenopathy associated with htlv-iii antibody positivity. vibrio vulnificus was isolated from the patient's blood and peritoneal fluid as well as from the same batch of oysters at the restaurant where the patient had visited. to our knowledge, ... | 1987 | 3817400 |
| processing-independent in vitro translocation of cytochrome p-450(scc) precursor across mitochondrial membranes. | in the presence of a membrane-permeable metal chelator, bovine adrenal cortex mitochondria imported p-450(scc) precursor without processing of the amino-terminal extension peptide. the imported precursor was bound to the matrix side surface of the inner membrane. when the inhibition due to the metal chelator was removed, the imported precursor was processed to the mature form. unprocessed precursor was also detected in mitochondria when the import reaction was carried out at relatively low tempe ... | 1986 | 3818579 |
| similarity principles and intrinsic geometries: contrasting approaches to interspecies scaling. | we criticize standard allometric approaches on the grounds that they emphasize scaling to one variable at a time, whereas chemically reactive hydrodynamic systems involved in pharmacokinetic phenomena are of higher dimension. we show that attempts based on mechanical similitude to set a dosage that would be equivalent across species (for example, from mouse to humans) lead to ambiguous results. another failing of standard allometry may be its incapability to accommodate the neoteny of homo sapie ... | 1986 | 3820095 |
| isolation and properties of a novel igg-binding protein from streptococci of serological group u. | a nonimmune binding of immunoglobulin (ig) g has been detected in streptococci of group u. the group u fc-binding site differed from the five previously known types of staphylococcal and streptococcal fc-binding sites by its strong affinity for murine igg, with dissociation constants in nanomolar range for rat and mouse igg, as well as for mouse igg subclasses 1, 2a, 2b and 3. it also differed from other binding sites by the high sensitivity towards trypsin. the fc-binding protein could be solub ... | 1987 | 3821654 |
| reaction to injectable collagen: results in animal models and clinical use. | since its commercial release, zyderm collagen implant has been used to treat more than 200,000 subjects in the united states for soft-tissue contour defects and more than 250,000 patients internationally (including the united states). approximately 3 percent of subjects' skin tested with zyderm collagen experience localized hypersensitivity reactions to collagen, whereas approximately 1 percent of treated patients demonstrate symptoms of hypersensitivity at treatment sites. of the latter treatme ... | 1987 | 3823250 |
| identification of environmental vibrio vulnificus isolates with a dna probe for the cytotoxin-hemolysin gene. | we screened 44 lactose-positive vibrio strains isolated from the marine environment for homology with a 3.2-kilobase dna fragment encoding the vibrio vulnificus cytotoxin-hemolysin gene. all 29 marine isolates identified as v. vulnificus on the basis of numerical taxonomy and dna-dna hybridization studies hybridized with the cytotoxin gene probe, as did all v. vulnificus reference strains. homologous gene sequences were identified in no other lactose-positive marine vibrio isolates nor in 10 oth ... | 1987 | 3827248 |
| [an experimental study on adhesion blocking in simultaneous injury of tendon and nerve]. | the formation of adhesions between simultaneously injured tendon and nerve with and without adhesion blocking materials was studied by using a rabbit model. a collagen membrane, produced from adult bovine corium and with a negative electric charge induced by chemical modification, was used as the blocking material. microangiography and histological studies were performed to investigate the vascular supply to the adhesions. the following results were obtained. blood vessel proliferation was obser ... | 1985 | 3831164 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | | 1985 | 3835674 |
| degenerative lesions of a coronary chemoreceptor and nearby neural elements in the hearts of victims of sudden death. | a coronary chemoreceptor and its neighboring nerves and ganglia were studied by serial sections of tissue taken from the hearts of sixteen victims of sudden unexpected death. either focal or extensive inflammatory destruction was present in the chemoreceptors of 13 of the 16 hearts, and similar abnormalities of local nerves and ganglia were present in all 16 hearts. since an identical coronary chemoreceptor in the dog has previously been shown to be the site of origin of a powerful reflex with m ... | 1986 | 3837961 |
| isolation and characterization of neuropeptide y from porcine intestine. | the isolation and primary structure of intestinal neuropeptide y (npy) is described. the peptide was purified from porcine intestinal extracts using a chemical assay and radioimmunoassay for npy. the amino acid sequence of this peptide is: tyr-pro-ser-lys-pro-asp-asn-pro-gly-glu-asp-ala-pro-ala-glu-asp-leu-ala- arg-tyr-tyr- ser-ala-leu-arg-his-tyr-ile-asn-leu-ile-thr-arg-gln-arg-tyr-nh2. this the structure of intestinal npy is identical to the npy of brain origin. | 1985 | 3838090 |
| chemoattractants in fibrotic disorders. | fibrosis represents an excessive deposition of connective tissue which impedes the normal functions of an organ or tissue. the mechanisms leading to this increased deposition of connective tissue may be similar to those occurring in normal wound repair. we have previously shown that the repair process involves the migration of connective tissue cells to the site of injury and their subsequent proliferation. one of the principal factors controlling these events appears to be the platelet-derived ... | 1985 | 3851719 |
| human c-myc and n-ras expression during induction of hl-60 cellular differentiation. | the genome of the human hl-60 promyelocytic leukemia cell contains amplified c-myc sequences and the transforming n-ras oncogene. the present study has monitored c-myc and n-ras expression in hl-60 cells during induction of myeloid and monocytic differentiation with dimethyl sulfoxide, hexamethylene bisacetamide, 12-o-tetradecanoylphorbol-13-acetate and 1,25 dihydroxy-vitamin d3. the results demonstrate that induction of hl-60 differentiation is associated with decreases in c-myc rna, while ther ... | 1985 | 3856429 |