| two generalized transducing phages in vibrio parahaemolyticus and vibrio alginolyticus. | two bacteriophages named phi vp253 and phi vp143 isolated after ultraviolet induction from lysogenic strains of vibrio parahaemolyticus have been shown to be generalized transducing phages. so far, seven different auxotrophic markers of a v. parahaemolyticus strain could be transduced at the frequencies ranging from 2.2 x 10(-7) to 7.5 x 10(-5) per infected cell at the m.o.i. of approximately 1.0. the phage phi vp143, but not phi vp253, lysed 20 of the 28 strains of v. alginolyticus and the occu ... | 1991 | 1808461 |
| [identification of species of microorganisms of the genus vibrio]. | during 1988, a study was made on 61 microorganisms, genus vibrio, which were received at the national reference laboratory for acute diarrheic diseases. pedro kouri institute of tropical medicine. of them, 46 were from children with acute diarrheic disease and 15 were isolated from the environment. by means of biochemical tests. 61 vibrio cholerae no. 01. 9 vibrio parahaemolyticus and 1 vibrio alginolyticus were identified. emphasis is placed upon the importance of keeping a systematic surveilla ... | 1991 | 1812527 |
| [a study on diarrhoea disease caused by vibrionaceae along coast the east of zhejiang province]. | a study of the etiology of 1028 cases of acute diarrhoea due to vibrionaceae was carried out between august 1988 to july 1990 along the coast of east zhejiang province. from 417 clinical cases (40.56%) were found. 590 strains (57.39%) of pathogenic vibrios among them, the detection rates of vibrio, aeromonas and plesionmonas were 48.14%, 44.74%, 7.12% respectively. there were 8 species of vibrio detected: v. parahemolyticus (63.73%), v. fluvialis (11.97%), v. alginolyticus (10.215), non-01 vibri ... | 1991 | 1816992 |
| structure of the rabbit phospholamban gene, cloning of the human cdna, and assignment of the gene to human chromosome 6. | we have isolated and characterized genomic dna clones encoding rabbit phospholamban. only a single gene for phospholamban was detected in the rabbit genome. the phospholamban gene of 13.2 kilobases contains only one 10.5-kilobase intron, which separates exonic sequences located in the 5'-untranslated region. two potential transcription initiation sites were mapped to 335 and 185 nucleotides upstream from the translation initiation site in the mrna or 239 and 89 nucleotides upstream from the exon ... | 1991 | 1828805 |
| complexity and sequence identification of 24 rat v beta genes. | twenty-four tcr v beta genes were cloned by anchored pcr from the lewis rat strain and identified by nucleotide and amino acid sequence comparisons to known mouse v beta genes. rat v beta genes exist in 17 single-member and 3 multimember subfamilies and exhibit 86 to 94 and 72 to 92% nucleotide and amino acid sequence similarities, respectively, to their mouse counterparts. a single rat gene, designated v beta 20, having no previously known mouse counterpart was identified; a closely related gen ... | 1991 | 1828824 |
| [mean values of risk factors for ischemic heart disease and their percentile distributions in the alma-ata population of men aged 20-54]. | the authors analyze the results of a one-stage epidemiological study of the open population of men aged 20-54 years (n-2886), carried out with a purpose of revealing coronary heart disease (chd) and its risk factors (rf). the study was performed with the aid of the standard who methods of epidemiological investigations. the prevalence of the main chd rf (arterial and borderline hypertension, overweight, tobacco-smoking, dyslipoproteinemias) was established. the mean values of rf in different age ... | 1991 | 1829274 |
| survivals of vibrio parahaemolyticus and escherichia coli in a gastropod mollusc, heminerita japonica. | vibrio parahaemolyticus strains d-3 and r-13 were found to be cleared within 7 days from a marine neritid gastropod mollusc, heminerita japonica, maintained in artificial seawater with salinities of 15, 25 and 35 permil (%) at 25 degrees c. escherichia coli strain ys-2 survived at a level of 10(2) colony forming units per gram in the mollusc maintained in 15% water for up to 14 days and fell to non-detectable level within 7 days in a 35% salinity group. the ability of h. japonica to clear these ... | 1991 | 1830783 |
| attachment of vibrio parahaemolyticus strains to estuarine algae. | attachment of vibrio parahaemolyticus strains to estuarine microalgae was examined in artificial seawater by viable counts of the organism and direct counts of the bacterial cells after immunoperoxidase staining. thermostable direct hemolysin (tdh)-producing and tdh-non-producing strains of v. parahaemolyticus were found to attach to five estuarine strains of navicula (diatom alga) in similar levels. the level of the bacterial attachment depended on salinity and temperature of the water, in whic ... | 1991 | 1830790 |
| ecological cycle of thermostable direct hemolysin-producing strains of vibrio parahaemolyticus in a brackish-water area with special reference to molluscs and attached microalgae. | prevalences of thermostable direct hemolysin (tdh)-producing strains in communities of a gastropod mollusc. clithon retropictus, and a bivalve mollusc, corbicula japonica, and levels of the strains in attached microalgae and muddy sediments were investigated at a brackish-water area along hashizu creek and togo pond in japan, v. parahaemolyticus was detected from attached microalgae at hashizu creek in summer months with the highest level of 1.4 x 10(5) cfu/g. levels of the organism among 20 ani ... | 1991 | 1830798 |
| in vitro attachment of vibrio parahaemolyticus to hemocytes of two gastropod molluscs. | vibrio parahaemolyticus d-3 was observed to attach to hemocytes of a marine gastropod mollusc, nerita albicilla, regardless of the presence of n. albicilla serum. the organism attached to hemocytes of an estuarine gastropod, clithon retropictus, in the presence of c. retropictus serum while the attachment to the hemocytes was decreased significantly in the absence of the serum. these evidences suggest that n. albicilla hemocytes would facilitate the clearance of v. parahaemolyticus from the alim ... | 1991 | 1830802 |
| na(+)-coupled atp synthesis in a mutant of vibrio parahaemolyticus lacking h(+)-translocating atpase activity. | an h(+)-translocating atpase-defective mutant of vibrio parahaemolyticus ys-1 grew well on lactate as a sole source of carbon at ph 8.5 under aerobic conditions, but not under anaerobic conditions. both wild type cells and the mutant cells could grow on lactate at ph 8.5 even in the presence of an h+ conductor, carbonylcyanide m-chlorophenylhydrazone (cccp), but not at ph 7.5. oxidative phosphorylation resistant to cccp in the mutant occurred at ph 8.5. these findings suggest the existence of na ... | 1991 | 1831976 |
| plasma-dependent chemotactic activity of hemocytes derived from a juvenile estuarine gastropod mollusc, clithon retropictus, to vibrio parahaemolyticus and escherichia coli strains. | hemocytes of adult clithon retropictus were attracted chemotactically to live vibrio parahaemolyticus and escherichia coli strains. the chemotaxis was stimulated by the plasma of adult c. retropictus. hemocytes of the juvenile specimen were attracted chemotactically to v. parahaemolyticus and e. coli strains in the presence of the plasma of the juvenile and only to e. coli strain in the absence of the plasma. these evidences suggest that hemocytes of juvenile c. retropictus might be defective to ... | 1991 | 1836376 |
| phosphate-dependent anti-vibrio parahaemolyticus activity in neritid gastropod molluscs. | | 1991 | 1836388 |
| [human gastroenteritis associated with vibrio parahaemolyticus in recife, brazil]. | a study was carried out on the occurrence of vibrio parahaemolyticus in 1,100 diarrheal feces, routinely sent to a private clinical laboratory for microbiologic diagnosis, in recife. v. parahaemolyticus was isolated from 14 (1.3%) fecal samples. however, if we considered only the specimens from adult patients, the isolation rate of v. parahaemolyticus rose to 7.1%. in most cases (92.86%), v. parahaemolyticus was the only enteropathogen recognized. among the isolates, seven k antigen serovars wer ... | 1991 | 1843400 |
| isolation of urease-positive vibrio parahaemolyticus from diarrheal patients in northeast brazil. | of 21 human fecal strains of vibrio parahaemolyticus, isolated on the northeast coast of brazil, eight (38%) were urease positive. most of these strains, in contrast to the urease-negative ones, did not produce the hemolysin responsible for the kanagawa phenomenon. | 1991 | 1844947 |
| growth arrest induced by transforming growth factor beta 1 is accompanied by protein phosphatase activation in human keratinocytes. | protein phosphorylation and dephosphorylation are involved in regulation of cell growth. we tested the hypothesis that the growth inhibitory effect of transforming growth factor beta 1 (tgf-beta 1) involves activation of protein phosphatases. exposure of human keratinocytes in culture to 400 pm tgf-beta 1 for 48 h led to 80% inhibition of dna synthesis as measured by nuclear labeling. incubation of cultured keratinocytes with 400 pm tgf-beta 1 rapidly activated (within 30 min) protein serine/thr ... | 1991 | 1847373 |
| development of circadian sleep-wake rhythms in preterm and full-term infants. | we have compared the roles of neurologic maturity and environmental time cues in the development of the entrained circadian sleep-wake rhythm in the preterm and term human infant. the preterm infants (n = 19) spent some time after birth in a hospital nursery with no environmental time cues, whereas the term infants (n = 22) were exposed from birth to a cyclical light and dark environment with one major caregiver. the circadian sleep-wake rhythm in the preterm infants entrained after a similar ti ... | 1991 | 1852533 |
| interaction of butorphanol, with monoamine oxidase inhibitor, tranylcypromine. | this investigation examines the possibility of interaction between tranylcypromine and butorphanol in comparison to pethidine. the ld50 of pethidine and butorphanol were determined in mice pretreated with the non-selective monoamine oxidase (mao) inhibitor, tranylcypromine orally for 8 days or with oral saline solution. tranylcypromine decreased the ld50 of both pethidine and butorphanol by 78% and 41%, respectively. anesthetized rabbits with halothane pretreated with tranylcypromine or saline w ... | 1991 | 1855717 |
| interaction of butorphanol, with monoamine oxidase inhibitor, tranylcypromine. | this investigation examines the possibility of interaction between tranylcypromine and butorphanol in comparison to pethidine. the ld50 of pethidine and butorphanol were determined in mice pretreated with the non-selective monoamine oxidase (mao) inhibitor, tranylcypromine orally for 8 days or with oral saline solution. tranylcypromine decreased the ld50 of both pethidine and butorphanol by 78% and 41%, respectively. anesthetized rabbits with halothane pretreated with tranylcypromine or saline w ... | 1991 | 1855717 |
| in vitro effect of phyllanthus amarus on hepatitis b virus. | to evaluate the effects of p. amarus on hepatitis b virus (hbv) antigens and hbv-dna, initial ethanolic extract and subsequent fractions of the plants were prepared. the whole plant material was dried, powdered and extracted with alcohol and subsequently fractionated in hexane, chloroform, butanol and finally in water. all the material were tested for in vitro effects on hbsag, hbeag and hbv-dna in serum samples positive for hbv antigens followed by the screening of respective antigens by elisa. ... | 1991 | 1855821 |
| relevance of n-nitrosamines to oesophageal cancer in china. | oesophageal cancer occurs at a very high frequency in certain areas of china, especially in linxian county, henan province. previous studies suggested that n-nitroso compounds play a causative role. in order to study further the exposure of linxian inhabitants, the intake of n-nitrosamines in the diet was determined. the total daily intake of volatile nitrosamines and of n-nitroso-n-methylbenzylamine (nmbza) was higher in linxian than in two other counties. nmbza can induce cancer in animal and ... | 1991 | 1855832 |
| similarity of the tdh gene-bearing plasmids of vibrio cholerae non-o1 and vibrio parahaemolyticus. | the gene encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus was previously cloned from a plasmid of vibrio cholerae non-o1. the gene (designated as nag-tdh) was subcloned and its nucleotide sequence was determined and compared with reported sequences of the four tdh gene copies encoding tdh, of which three were cloned from the chromosome and one was cloned from a plasmid of v. parahaemolyticus. in the coding region, the nag-tdh gene had 100% homolo ... | 1991 | 1857199 |
| animal rights. | | 1991 | 1865899 |
| an evaluation of three commercial fecal transport systems for the recovery of enteric pathogens. | twenty-five isolates, including six strains of shigella species, six strains of salmonella species, five strains of yersinia enterocolitica, six strains of campylobacter jejuni, and two strains of vibrio parahaemolyticus, were inoculated at a concentration of 1.5 x 10(4) colony-forming units/ml into the following transport systems: fekal enteric plus (trend scientific, inc., st. paul, mn), cary blair transport medium (remel, inc., lenexa, ks), and para-pak c & s (meridian diagnostics, inc., cinc ... | 1991 | 1877533 |
| analysis of the gene of vibrio hollisae encoding the hemolysin similar to the thermostable direct hemolysin of vibrio parahaemolyticus. | the gene (designated as vh-tdh) of vibrio hollisae 9041 encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of v. parahaemolyticus contained a 567-base-pair open reading frame (orf), which was 93.3-93.5% homologous to those of the tdh genes of v. parahaemolyticus, v. cholerae non-01, and v. mimicus encoding tdh or similar hemolysins. comparative analysis of the nucleotide sequence containing the vh-tdh orf with published nucleotide and amino acid sequences suggested that the ... | 1991 | 1884983 |
| functional expression of furin demonstrating its intracellular localization and endoprotease activity for processing of proalbumin and complement pro-c3. | we have cloned a rat cdna encoding furin which is structurally related to yeast kex2 protease. products of 88 and 94 kda were obtained by in vitro transcription/translation of the cdna in the absence and presence of microsomes. when the cdna was transfected into cos-1 cells, furin was expressed as a major glycosylated form of 94 kda, accompanied by a minor proteolytic form of 86 kda, and found to be localized in the golgi complex. proalbumin and complement pro-c3 are intracellularly processed in ... | 1991 | 1885622 |
| effects of environmental exposure on carbon polysulphone composites. | in vivo and in vitro studies were designed to characterize the material degradation associated with implantation of carbon fibre-reinforced polysulphone (c/ps). composite plates were compression moulded for both studies, and a fibre orientation of 0 degrees with the long axis and a fibre volume fraction of approximately 55% were used. the in vitro experiment involved soaking three groups of four plates each at 4, 12 and 26 wk in 0.9% saline solution at 37 degrees c. the plates were measured for ... | 1991 | 1888812 |
| characterization of thermostable direct hemolysins encoded by four representative tdh genes of vibrio parahaemolyticus. | four tdh genes encoding thermostable direct hemolysin (tdh) cloned from two representative strains of v. parahaemolyticus (tdh 1 and tdh 2 from a hemolytic strain, tdh3 and tdh4 from a non-hemolytic strain) have different nucleotide sequences (the maximum divergence: 3.3%). in this study, each tdh gene product was purified from the lysate of escherichia coli cells carrying the cloned gene and their properties were compared to investigate the influence of the amino acid substitutions caused by th ... | 1991 | 1890953 |
| comparison of the indirect immunobead, radiolabeled, and immunofluorescence assays for immunoglobulin g serum antibodies to human sperm. | the relative sensitivities of the indirect immunobead test, the indirect flo cytometric immunofluorescence assay, and an indirect radiolabeled antiglobulin assay were compared. eighteen immunobead test positive sera and 18 negative sera were used as the standard for the other two assays. of the 18 positive sera, 14 (77%) and 5 (27%) were positive in the immunofluorescence assay and the radiolabeled antiglobulin assay, respectively. four (22%) of the low titer immunobead test positive sera were n ... | 1991 | 1899399 |
| comparative characterization of inducible and virulent vibrio parahaemolyticus bacteriophages having unique head projections. | phage tp1, induced from vibrio parahaemolyticus k-20 pilot strain by mitomycin c, exhibited a unique hexagonal head with knob-like projections which covered the whole capsid and a noncontractile tail. the appearance of this phage was very similar to those of phages vp3 and vp6, isolated from seawater. the host range of phage tp1 was similar to those of phages vp3 and vp6. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of the three phage particles revealed almost identical p ... | 1991 | 1908040 |
| analysis of the tdh gene cloned from a tdh gene- and trh gene-positive strain of vibrio parahaemolyticus. | a variant of the gene (tdh) encoding thermostable direct hemolysin (tdh) was cloned from the chromosome of vibrio parahaemolyticus aq3860, which gave positive results in the hybridization tests with the tdh gene probe and the trh (tdh-related hemolysin) gene probe and showed a low level of reaction in an enzyme-linked immunosorbent assay for tdh. nucleotide sequence analysis of the cloned gene (tdh5) provided no evidence that tdh5 is evolutionally closer to the trh gene than the other tdh genes. ... | 1991 | 1908042 |
| the occurrence and growth of microorganisms during the fermentation of fish sausage. | minced fish (mullet) sausage mixes containing added sugar, salt, nitrate, nitrite and spices were fermented (48 h, 30 degrees c) by indigenous flora or by a starter culture (pediococcus acidilactici) and the microbial ecology and behaviour of various bacteria was monitored. pediococcus pentosaceus and lactobacillus plantarum dominated the indigenous fermentation, achieving populations of 10(7)-10(8) cfu/g by 48 h, and decreasing the ph of the mix to 4.5-4.7. significant growth (10(5)-10(7) cfu/g ... | 1991 | 1909546 |
| cholera and vibrio parahaemolyticus diarrhoea endemicity in calabar, nigeria. | the microbiological and morbidity profiles of acute diarrhoeal episodes were studied in 881 patients seen at the out-patients department of the university of calabar teaching hospital (ucth), calabar, between january and december, 1988. of a total of 108 (12.3%) culturally confirmed bacterial diarrhoeas, 47 (43.5%) were due to escherichia coli, 33 (30.6%) to vibrios (vibrio cholerae-01; classical and e1 tor biotypes and v. parahaemolyticus), while shigella spp. and salmonella. spp. accounted for ... | 1991 | 1911487 |
| a novel mechanism of cation/substrate cotransport: na+/h+/adenosine cotransport in vibrio parahaemolyticus. | adenosine is actively transported with na+ in vibrio parahaemolyticus (sakai, y., tsuda, m., tsuchiya, t. (1987) biochim, biophys. acta 893, 43-48). the proton conductor carbonylcyanide m-chlorophenylhydrazone, cccp, strongly inhibited active transport of adenosine at ph 8.5 as well as at ph 7.0. this seemed peculiar because the driving force, an electrochemical potential of na+, is established by the na(+)-extruding respiratory chain at ph 8.5 in this organism, although it is established by the ... | 1991 | 1911824 |
| formycin a resistant mutants due to defect in adenosine transport system in vibrio parahaemolyticus. | an antibiotic formycin a inhibited growth of vibrio parahaemolyticus under certain conditions, which suggested that formycin a was taken up by cells under these conditions. we found that formycin a was transported via the adenosine transport system which we previously reported as a na(+)-coupled cotransport system. we isolated many formycin a resistant mutants, and about half of them grew very poorly on adenosine as a sole source of carbon. judging from their reversion frequencies, these mutants ... | 1991 | 1914010 |
| occurrence of 2-keto-3-deoxy-d-manno-octonic acid in lipopolysaccharides isolated from vibrio parahaemolyticus. | the occurrence of 2-keto-3-deoxy-d-manno-octonic acid (kdo) in lipopolysaccharides (lps) of vibrio parahaemolyticus was demonstrated for the first time by gas chromatography-mass spectrometry after dephosphorylation, reduction, and methylation. kdo was virtually completely phosphorylated, since no kdo was detected by either gas chromatography or thiobarbituric acid assay before dephosphorylation. the level of kdo in all six strains of v. parahaemolyticus investigated ranged from 0.37 to 0.69%, w ... | 1991 | 1917863 |
| optical sectioning of microbial biofilms. | scanning confocal laser microscopy (sclm) was used to visualize fully hydrated microbial biofilms. the improved rejection of out-of-focus haze and the increased resolution of sclm made it preferable to conventional phase microscopy for the analysis of living biofilms. the extent of image improvement was dependent on the characteristics of individual biofilms and was most apparent when films were dispersed in three dimensions, when they were thick, and when they contained a high number of cells. ... | 1991 | 1917879 |
| tryptophan 65 is essential for hemolytic activity of the thermostable direct hemolysin from vibrio parahaemolyticus. | the effect of modification of aromatic and ionizable amino acid residues on the hemolytic activity of a thermostable direct hemolysin from vibrio parahaemolyticus was examined. tryptophan 65, one of the two tryptophan residues per subunit, was specifically modified with n-bromosuccinimide, resulting in complete loss of hemolytic activity. however, neither nitration with tetranitromethane of one of the nine tyrosine residues nor nlm-ethoxyformylation of two of the four histidine residues caused a ... | 1991 | 1926183 |
| acute amphetamine-induced subsensitivity of a10 dopamine autoreceptors in vitro. | extracellular recordings were obtained from spontaneously active, presumed dopamine (da) neurons of the ventral tegmental area (vta) of the rat in a slice preparation. bath-applied (+)-amphetamine (amph) (1-30 microm) induced a concentration-dependent decrease in the firing rate of these neurons, which tended to saturate with the highest concentrations used (n = 11). this inhibitory effect was dependent on the activation of d2 receptors since it was reversed by the d2 antagonist sulpiride (n = 8 ... | 1991 | 1933376 |
| measurement of sensitivity to adriamycin in hybrid spheroids. | using the newly developed hybrid spheroid system, we have been able to measure sensitivities to adriamycin in two sublines of lovo cells (a human colon adenocarcinoma line). hybrid spheroids are composed of coagglomerated lovo cells, and nonproliferating hela "feeder" cells, mimicking a population of minitumors. spheroids of uniform size, but containing different numbers of lovo cells, were incubated with various concentrations of adriamycin and after washing, plated for colony formation. binomi ... | 1991 | 1933483 |
| percutaneous coronary and peripheral angioscopy with saline solution and carbon dioxide gas in porcine and canine arteries. | to obtain a clear view with angioscopy, blood must be displaced. carbon dioxide (co2) gas has proved to be a safe intraarterial contrast agent, and it may have advantages over saline solution as an infusion medium for angioscopy. this study compared the use of co2 gas and saline solution in the femoral artery, in the presence and absence of a proximal occlusion, in nine pigs and six dogs. the applicability of co2 gas in the coronary arteries was also evaluated. in total 185 angioscopy procedures ... | 1991 | 1950994 |
| stability of streptococcal pyrogenic exotoxin production with laboratory manipulation of group a streptococci. | because of reported differences in the production of streptococcal pyrogenic exotoxins by group a strains associated with severe streptococcal infections, the stability of exotoxin production by specific strains was examined by passing group a streptococci on blood agar culture plates daily for 20 days. no changes were detected in either exotoxin genes or in exotoxin production during this time, suggesting that these reported differences are due to other explanations such as differences in the s ... | 1991 | 1955721 |
| purification and characterization of a nuclease (3'-nucleotidase) from a penicillium sp. | a nuclease (3'-nucleotidase) similar to p1 nuclease from penicillium citrinum was purified from a commercial digestive from a penicillium sp. the activity of the nuclease (pa) was separated to three fractions by diethylaminoethyl-toyopearl 650m column chromatography, in total yield of 10%. the apparent molecular weight of these three nucleases, pa1, pa2 and pa3 was 35000, 33000, and 32000, respectively. all of them were homogeneous so far as checked by sodium dodecyl sulfate slab gel electrophor ... | 1990 | 1964878 |
| pili of a vibrio parahaemolyticus strain as a possible colonization factor. | pili of vibrio parahaemolyticus were purified from a kanagawa phenomenon-positive strain (ha7) that belongs to serogroup o2:k3 and is adhesive to rabbit intestine. the organisms treated with the fab fraction of antipilus antibody failed to adhere to the intestine. purified pili had the ability to adhere to the intestine, but the pretreatment of the intestine with purified pili did not allow adherence of the organisms to the intestine. these results suggest that pili of this v. parahaemolyticus s ... | 1990 | 1967172 |
| piliated vibrio parahaemolyticus adherent to human ureteral mucosa. | | 1990 | 1967625 |
| pathogenic escherichia coli, yersinia enterocolitica, and vibrio parahaemolyticus. | | 1990 | 1977991 |
| sequence analysis of nuta gene encoding membrane-bound cl(-)-dependent 5'-nucleotidase of vibrio parahaemolyticus. | the membrane-bound 5'-nucleotidase of vibrio parahaemolyticus is unique in requiring cl- for activity. we cloned the nuta gene encoding the 5'-nucleotidase and sequenced it. it contained an open reading frame consisting of 1,680 nucleotides capable of encoding a protein of 560 amino acid residues. the first 21 amino acid residues of the n-terminal portion of this protein seem to be a signal peptide. the rest of the polypeptide (539 residues) is hydrophilic, and its molecular weight was calculate ... | 1991 | 2016269 |
| characterization of a new thermostable direct haemolysin produced by a kanagawa-phenomenon-negative clinical isolate of vibrio parahaemolyticus. | the production of two haemolysins, thermostable direct haemolysin (vp-tdh) and a vp-tdh-related haemolysin (vp-trh), by clinical isolates of vibrio parahaemolyticus has previously been reported. here we describe a third type of haemolysin (named vp-tdh/i), which is produced by a clinical isolate (strain th012) that is kanagawa phenomenon negative. vp-tdh/i was purified by a series of column chromatographies on deae-sephadex a25, hydroxyapatite, sepharose 4b and mono q. by physicochemical, biolog ... | 1991 | 2016584 |
| experimental liver cysts induced by 2-acetylaminofluorene. | liver cyst formation was studied serially in an experimental model in which rats were fed a diet containing 0.02% 2-acetylaminofluorene, a carcinogen, for 6 weeks, followed by a normal diet for 42 weeks. cysts appeared in the portal tracts at the 12th week, by which time the intrahepatic bile ducts had also proliferated and dilated. some of the dilated bile ducts were cavernous or multicystic and appeared to represent a transitional form between the bile duct dilation and cystic formation. after ... | 1991 | 2040399 |
| abrogation of dominant glucose intolerance in sjl mice by a growth hormone transgene. | glucose tolerance was studied in transgenic mice (sjl x c57bl/6) expressing human gh under the control of a housekeeping promoter. parental sjl mice were found to harbour a dominant allele, termed here glid, determining glucose intolerance in pure-bred animals and in f1 hybrids with glucose-tolerant c57bl/6 mice. blood glucose levels in transgenic sjl x c57bl/6 hybrid mice were well controlled following glucose challenge, whereas non-transgenic hybrids failed to control their glucose adequately. ... | 1991 | 2043241 |
| the economics of spaying and neutering: market forces and owners' values affecting pet population control. | | 1991 | 2045338 |
| [the prevalence of vibrio parahaemolyticus and its antibodies in seafood handlers in mérida, yucatán]. | we report the prevalence of vibrio parahaemolyticus and of antibodies against vibrio parahaemolyticus in the feces and serum of fish and seafood handlers in the city of mérida, yucatán, mexico. between march 1 and august 31, 1989, we studied 81 feces samples and 81 serum samples from an equal number of handlers. vibrio parahaemolyticus was not isolated in any of the feces studied. we found no statistically significant differences upon comparing our zero per cent isolation in feces samples with t ... | 1991 | 2053021 |
| [surveys on the contamination of marine fish with non-o1 vibrio cholerae and vibrio mimicus and food poisoning cases by these organisms]. | the present paper describes the relationship between the contamination with non-o1 vibrio cholerae and vibrio mimicus of marine fish, with special reference to the seasonal variation and the concentration of contamination, and the actual cases of domestic food poisoning by these organisms. a 10 year survey revealed that non-o1 vibrio cholerae (non-o1 v. cholerae) strains were frequently isolated from fish during the summer season with some variations from one year to another, and isolates from f ... | 1991 | 2066602 |
| serum fructosamine and lipid profile in type i diabetic children in turkey. | serum levels of total cholesterol (tc), triglycerides (tg), lipoprotein cholesterol fractions (hdl-c and ldl-c) and fructosamine (fa) were determined in twenty-three children with type i diabetes and twenty healthy children aged 4-14 years. mean serum tc and hdl-c did not differ significantly between diabetic and nondiabetic children. mean serum tg and fa showed a statistically significant increase in the diabetic group compared to healthy children. it is interesting to note that extremely high ... | 1990 | 2075022 |
| [recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation]. | every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ... | 1990 | 2075365 |
| intraradicular bacteria and fungi in root-filled, asymptomatic human teeth with therapy-resistant periapical lesions: a long-term light and electron microscopic follow-up study. | light and electron microscopy were used to analyze nine therapy-resistant and asymptomatic human periapical lesions, which were removed as block biopsies during surgical treatment of the affected teeth. the cases that required surgery represented about 10% of all of the cases which received endodontic treatment and root fillings during the period 1977 to 1984. these cases revealed periapical lesions when they were examined 4 to 10 yr after treatment. the biopsies were processed for correlated li ... | 1990 | 2094761 |
| determination by a site-directed mutagenesis method of active site of thermostable direct hemolysin (tdh) produced by vibrio parahaemolyticus. | | 1990 | 2101139 |
| etiological agents of diarrhoea. | two decades of research have established newer pathogens and techniques in establishing several organisms of diarrhoeal diseases as aetiological agents. it is now possible to detect an agent in 80% of the situation of diarrhoea in a standard laboratory. the brief review describes the list of pathogens, their diagnostic techniques with short description on clinical and epidemiological status. | 1990 | 2101388 |
| properties of a hemolysin related to the thermostable direct hemolysin produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus. | a hemolytic toxin (vp-trh) produced by a kanagawa phenomenon negative, clinical isolate of vibrio parahaemolyticus was further characterized. the purified vp-trh showed various biological activities, such as fluid accumulation in rabbit ileal loops, increase of rabbit skin vascular permeability, and cardiotoxicity on cultured myocardial cells, all of which are essentially similar to the activities found with thermostable direct hemolysin (vp-tdh), a pathogenic toxin produced by kanagawa phenomen ... | 1990 | 2118824 |
| photosystem ii characteristics of a constructed synechocystis 6803 mutant lacking synthesis of the d1 polypeptide. | photosystem ii (psii) composition was studied in a mutant of the cyanobacterium synechosystis 6803 in which synthesis of the reaction center polypeptide d1 has been inactivated. the mutant thylakoids had lost also the other reaction center polypeptide d2 and the chlorophyll alpha-binding protein cp47. cytochrome b559 and the chlorophyll alpha-binding protein cp43 accumulated to almost wild-type amounts in mutant thylakoids. also the 33 kda polypeptide involved in water oxidation was present and ... | 1990 | 2129398 |
| structural and functional analysis of the first intron of the human alpha 2(i) collagen-encoding gene. | the nucleotide (nt) sequence of the first intron of the human alpha 2(i) collagen-encoding gene (col1a2) has been determined from its 5' terminus (nt 207) to nt 2045 with respect to the transcription start point. although the first intron contains elements known to function in transcriptional regulation of other genes (two ap1-binding sites and an alternating gt stretch), comparison of this sequence with that of the mouse col1a2 first intron revealed a low degree of nt sequence identity and very ... | 1990 | 2129528 |
| yolk degradation in tick eggs: ii. evidence that cathepsin l-like proteinase is stored as a latent, acid-activable proenzyme. | cathepsin l-like proteinase found in the eggs of the tick ornithodoros moubata is latent during embryogenesis, but can be activated by acid treatment. in crude extracts as well as in partially purified fractions, activation requires reducing conditions and is inhibited by leupeptin, which indicates that it is mediated by a thiol proteinase, probably by the cathepsin l itself. latency disappears in vivo at the time of the acute phase of yolk digestion, which takes place during late embryonic deve ... | 1990 | 2134179 |
| [isolation of enteropathogenic vibrio in bivalves and mud from the nicoya gulf, costa rica]. | the presence of enteropathogenic vibrio was evaluated in 36 sediment samples and 41 bivalve samples obtained from 3 collecting sites in the golfo de nicoya, costa rica. isolation methods for halophilic and non halophilic vibrio were used. the biochemical profiles of the strains obtained revealed the presence of the following isolates: 224 vibrio parahaemolyticus, 3 v. furnissii, 1 v. damsela and 3 v. fluvialis. v. cholerae was not isolated, due principally to the use of tcbs agar. | 1990 | 2134726 |
| characteristics of the h(+)-translocating adenosine triphosphatase of vibrio parahaemolyticus. | we have characterized h(+)-translocating adenosine triphosphatase (atpase) in membrane vesicles of vibrio parahaemolyticus. the atpase required high concentrations (about 0.5 m) of na2so4 (or other salts) for its maximum activity. magnesium ion stimulated the atpase activity, but ca2+ did not. the activity of atpase was inhibited by tetrachlorosalicylanilide, an h+ conductor, but not by another h+ conductor, carbonylcyanide-m-chlorophenylhydrazone. the activity was strongly inhibited by dicycloh ... | 1990 | 2140074 |
| [spectroscopy of the liver in vivo using nuclear magnetic resonance. a new approach in hepatic physiopathology]. | nuclear magnetic resonance (nmr) spectroscopy is a non-invasive means of detecting numerous compounds, thus enabling real-time studies to be carried out on such topics as energy metabolism, intracellular ph and main intermediate metabolism pathways. this method has been used for several years to study perfused livers and rat livers in vivo, but its application to man has just begun. we describe some of its principal uses, with emphasis on the possibilities it offers to investigate the metabolic ... | 1990 | 2140172 |
| [antibiotic sensitivity of vibrio parahaemolyticus isolated in the turkmene ssr]. | sensitivity of 82 cultures of parahemolytic vibrios to 8 antibiotics was studied. it was shown that the majority of the strains were highly sensitive to levomycetin and gentamicin, sensitive to tetracycline, rifampicin, streptomycin, neomycin and kanamycin and resistant to ampicillin. | 1990 | 2141785 |
| rapid purification and characterization of f1-atpase of vibrio parahaemolyticus. | the f1 portion of h(+)-translocating atpase as purified from membrane vesicles of vibrio parahaemolyticus by a rapid procedure. the whole purification process (from culture of cells to purification of the enzyme) could be completed in 1 day. the f1-atpase consists of five subunits (alpha, beta, gamma, delta and epsilon) like f1 of escherichia coli and other microorganisms. the f1-atpase of v. parahaemolyticus showed some interesting properties. its activity was greatly stimulated by high concent ... | 1990 | 2142893 |
| modulation of experimentally induced epilepsy by intracerebral grafts of fetal gabaergic neurons. | systemic administration of pilocarpine to rats induces seizures that resemble complex partial epilepsy in humans. susceptibility to these seizures is increased by lesion of the gabaergic striatonigral projection. transplantation of fetal gabaergic neurons, but also of control non-gabaergic tissue, to the deafferent substantia nigra can reduce such lesion-increased seizure susceptibility. these observations are consistent with prior evidence that gabaergic basal ganglia outflow plays an important ... | 1990 | 2168529 |
| mechanisms underlying the stimulation of pth release by gppnhp in permeabilized bovine parathyroid cells. | we examined changes in camp and inositol phosphate metabolism to assess the contribution of the guanine nucleotide regulatory (g) protein(s) regulating adenylate cyclase and phospholipase c in mediating the stimulatory effects of gppnhp on pth release from permeabilized bovine parathyroid cells. to examine the role of gs, the g protein stimulating adenylate cyclase, and camp on pth release, permeabilized cells were incubated with either gppnhp or isoproterenol, and the effects of these agents on ... | 1990 | 2168660 |
| downregulation of parathyroid hormone receptors in renal membranes from aged rats. | the mechanism of the inhibition or blunting of parathyroid hormone (pth)-stimulated na(+)-ca2+ exchange activity in renal cortical cells from aged rats was examined. the number of pth binding sites in basolateral membranes prepared from adult (6 mo) and old (24 mo) rats was quantitated by the binding of the synthetic analogue 125i-labeled [nle8,18, tyr34]bpth-(1-34) amide to the membrane. the maximum number of specific pth binding sites, bmax, was 92.7 +/- 9.3 and 36.7 +/- 6.1 fmol/mg protein, r ... | 1990 | 2168678 |
| from the national institute of allergy and infectious diseases. summary of the 25th united states-japan joint conference on cholera and related diarrheal diseases. | | 1990 | 2201739 |
| migratory deficiency of clithon retropictus hemocytes to vibrio parahaemolyticus and escherichia coli. | hemocytes of a marine gastropod, nerita albicilla, but not those of an estuarine gastropod, clithon retropictus, were observed to migrate to live and heat-killed cells of vibrio parahaemolyticus and escherichia coli through nucleopore membrane in blind well chamber. the defective migration of c. retropictus hemocytes might reflect, at least in part, the survival of v. parahaemolyticus in the estuarine gastropod. | 1990 | 2202845 |
| water associated infections. | | 1990 | 2216140 |
| cloning and expression of two genes encoding highly homologous hemolysins from a kanagawa phenomenon-positive vibrio parahaemolyticus t4750 strain. | we have cloned and sequenced the gene encoding thermostable direct hemolysin (tdh), a possible virulence factor in vibrio parahaemolyticus gastroenteritis, from a kanagawa-phenomenon-positive strain, t4750. this strain was found to contain two sequences (tdha and tdhs) homologous to the tdh gene previously reported by nishibuchi and kaper [j. bacteriol 162 (1985) 558-564] and taniguchi et al. [microb. pathog. 1 (1986) 425-432]. sequence homology of the coding regior between tdha and tdhs was 97. ... | 1990 | 2227429 |
| a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india. | chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ... | 1990 | 2227713 |
| a new species of the genus chauhanellus bychowsky et nagibina, 1969 (monogenea) from the gills of a sea fish tachysurus dussumieri (val.) from andhra pradesh, india. | chauhanellus chauhani sp. n., collected from the gills of a sea cat-fish, tachysurus dussumieri (valenciennes) from the bay of bengal, off the coast of kakinada, andhra pradesh, india is described and illustrated. it differs from the related species of the genus--c. oculatus, c. flexiosus, c. australis, c. nagibinae, and c. pedunculatus--in the shape and articulation of accessory piece with cirrus and in having a crescentic posteromedian process on its dorsal bar. it has five transverse hard str ... | 1990 | 2227713 |
| molecular epidemiologic evidence for association of thermostable direct hemolysin (tdh) and tdh-related hemolysin of vibrio parahaemolyticus with gastroenteritis. | the kanagawa phenomenon induced by the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus is almost exclusively associated with clinical strains, and tdh has been considered an important virulence factor. however, kanagawa phenomenon-negative strains isolated from patients with diarrhea have recently been shown to produce tdh-related hemolysin (trh). we studied the distribution of the tdh gene encoding tdh and the trh gene encoding trh in vibrios by hybridization analyses. the presen ... | 1990 | 2228229 |
| [development and application of enzyme-linked immunosorbent assay using monoclonal antibody against a hemolysin (vp-trh) of vibrio paraheamolyticus--evidence that vp-trh producing-kanagawa phenomenon-negative v. parahaemolyticus is a human pathogen]. | although it has been well established that kanagawa phenomenon-positive vibrio parahaemolyticus is a human enteropathogen, the kanagawa phenomenon-negative one has been considered to be probably not pathogenic. we have found, however, an outbreak of gastroenteritis due to kanagawa phenomenon-negative v. parahaemolyticus which produces a new toxin (vp-trh) resembling to vp-tdh, a responsible toxin of kanagawa phenomenon. in this study, we developed monoclonal antibodies against vp-trh which were ... | 1990 | 2230365 |
| surface-induced swarmer cell differentiation of vibrio parahaemolyticus. | vibrio parahaemolyticus distinguishes between life in a liquid environment and life on a surface. growth on a surface induces differentiation from a swimmer cell to a swarmer cell type. each cell type is adapted for locomotion under different circumstances. swimmer cells synthesize a single polar flagellum (fla) for movement in a liquid medium, and swarmer cells produce an additional distinct flagellar system, the lateral flagella (laf), for movement across a solid substratum, called swarming. r ... | 1990 | 2233248 |
| [effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog]. | a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ... | 1990 | 2238764 |
| [clinical, bacteriological, and immunological examination and treatment of two papillon-lefevre syndrome patients]. | papillon-lefèvre syndrome (pls) is a rare disease accompanied by palmo-plantar hyperkeratosis and rapidly progressive periodontal breakdown of deciduous and permanent dentition. two unrelated female pls patients, four and seven years old, with severe periodontal destruction were examined. antibody titers against seven strains by the enzyme-linked immunosorbent assay (elisa), microbial cultures from deep periodontal pockets and mouth rinse samples and immunoblotting analysis were performed. titer ... | 1990 | 2258685 |
| [clinical, bacteriological, and immunological examination and treatment of two papillon-lefevre syndrome patients]. | papillon-lefèvre syndrome (pls) is a rare disease accompanied by palmo-plantar hyperkeratosis and rapidly progressive periodontal breakdown of deciduous and permanent dentition. two unrelated female pls patients, four and seven years old, with severe periodontal destruction were examined. antibody titers against seven strains by the enzyme-linked immunosorbent assay (elisa), microbial cultures from deep periodontal pockets and mouth rinse samples and immunoblotting analysis were performed. titer ... | 1990 | 2258685 |
| [investigation on the distribution and serotyping of v. parahaemolyticus isolated from marine products and environment in zhoushan archipelago]. | isolation for v. parahaemolyticus from 1003 samples of marine products and environment was attempted in zhoushan archipelago in 1986-1987. the results showed that v. parahaemolyticus could be detected from all kinds of marine products, the positive rate was between 40%-100%. the v. parahaemolyticus was detected in all samples from the environment. the main o groups were o2, o5, o1, o4, o3 and o12, the o9 groups was not detected. although 103 serotypes have been detected, the main serotypes were ... | 1990 | 2261620 |
| effect of norspermidine and its related triamines on the cell-free polyphenylalanine synthesizing system from vibrio parahaemolyticus. | the effect of norspermidine and its structurally related triamines on the cell-free polyphenylalanine synthesizing system from vibrio parahaemolyticus was examined in connection with the requirement of the system for monovalent cation. in the absence of norspermidine, the maximal incorporation of [14c]phenylalanine into hot trichloroacetic acid insoluble material was observed under ionic conditions of 12 mm mg2+ and 50 mm nh4+. k+ could partially substitute for nh4+, but na+ could not. the addit ... | 1990 | 2266881 |
| natural plasmid transformation in a high-frequency-of-transformation marine vibrio strain. | the estuarine bacterium vibrio strain di-9 has been shown to be naturally transformable with both broad host range plasmid multimers and homologous chromosomal dna at average frequencies of 3.5 x 10(-9) and 3.4 x 10(-7) transformants per recipient, respectively. growth of plasmid transformants in nonselective medium resulted in cured strains that transformed 6 to 42, 857 times more frequently than the parental strain, depending on the type of transforming dna. these high-frequency-of-transformat ... | 1990 | 2268155 |
| survey of enteropathogenic agents in children with and without diarrhoea in ghana. | a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ... | 1990 | 2270006 |
| vibrio infections in kansas city. | in august 1989, the kansas city health department declared vibrio infections a reportable disease. the authors present a report discussing cases of vibriosis reported between 1980 and 1989. | 1990 | 2270070 |
| nucleotide sequence of the thermostable direct hemolysin gene (tdh gene) of vibrio mimicus and its evolutionary relationship with the tdh genes of vibrio parahaemolyticus. | the gene encoding a hemolysin similar to the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus was previously cloned from the chromosome of vibrio mimicus. the nucleotide sequence of the hemolysin gene was determined in this study. the gene proved to be a variant of the thermostable direct hemolysin gene (tdh gene) and was designated as vm-tdh because the sequence divergences between the vm-tdh gene and four tdh genes of v. parahaemolyticus were 2.1-3.0%, while the sequence divergen ... | 1990 | 2272498 |
| genetic transformation of vibrio parahaemolyticus, vibrio alginolyticus, and vibrio cholerae non o-1 with plasmid dna by electroporation. | an electroporation procedure for the plasmid-mediated transformation of the genus vibrio was performed, as part of an effort to develop recombinant dna techniques for genetic manipulation of the genus vibrio. vibrio parahaemolyticus, v. alginolyticus, and v. cholerae non o-1 (9 different strains) were transformed with 3 vector plasmids (pacyc184, phsg398, and pbr325). the efficiency of transformation was highly dependent on three parameters: the concentration of plasmid dna; the strength of the ... | 1990 | 2280728 |
| biotin uptake in cultured hepatocytes from normal and biotin-deficient rats. | biotin uptake was studied in isolated cultured hepatocytes of normal and biotin-deficient rats. biotin uptake was temperature-dependent with respect to physical, but not to chemical, processes, proportional to the exogenous biotin concentration in the medium, independent of ph and sodium ion concentrations of the medium, and uneffected by the presence of structural analogues of biotin or metabolic inhibitors in both normal and biotin-deficient hepatocytes. these results suggest that biotin uptak ... | 1990 | 2288769 |
| generation of affinity for antithrombin iii by supplemental sulfation of heparin species with low affinity for the protein. | the tributylammonium salt of a porcine heparin subfraction with low affinity for antithrombin iii (mr 7,500-18,000; anti-clotting activity, 7 usp units/mg), having degrees of sulfate substitution at d-glucosamine and l-iduronic acid residues of glcns 0.786, glcn6s 0.628, and idoa2s 0.682 mol, was reacted with 10 or 20 mol of pyridine-sulfur trioxide per mol equiv. of available hydroxyl groups in n,n-dimethylformamide at -10 degrees c for 1 h. both chemical and nmr spectroscopic analyses revealed ... | 1990 | 2292586 |
| chemical modification of pig liver initiation factor eif-2 with n-ethylmaleimide. amino acid sequences around the n-ethylmaleimide-reactive sulfhydryl groups and the effect of gdp on the modification. | the activity of eukaryotic initiation factor eif-2 as to the formation of the ternary complex, eif-2 gtp met-trna(f), is inhibited by n-ethylmaleimide. our preparation of pig liver eif-2 contained alpha and gamma subunits and was inhibited by more than 90% by n-ethylmaleimide. using our eif-2, we determined the sequences around the n-ethylmaleimide-reactive sulfhydryl groups, studied the effect of gdp on the sulfhydryl modification and that of nem on the [3h]gdp binding, and examined the protect ... | 1990 | 2292592 |
| [human immunodeficiency virus infection associated with tuberculosis]. | in order to detect an association between hiv infection and tuberculosis (tb), 130 tb inpatients were studied one of whom presented a pulmonary disease due to mycobacterium avium intracellulare. all had advanced tb, 95.4%, with pulmonary localization. serum anti-hiv antibodies were detected by elisa and their presence confirmed by immunoblotting in 4 (3.1%) individuals, three males and one female, with different degrees of pulmonary tb. of the males, 1 was bisexual, 2 were promiscuous, and the f ... | 1990 | 2292908 |
| assay of all-trans----11-cis-retinoid isomerase activity in bovine retinal pigment epithelium. | | 1990 | 2292965 |
| chemotactic control of the two flagellar systems of vibrio parahaemolyticus. | vibrio parahaemolyticus synthesizes two distinct flagellar organelles, the polar flagellum (fla), which propels the bacterium in a liquid environment (swimming), and the lateral flagella (laf), which are responsible for movement over surfaces (swarming). chemotactic control of each of these flagellar systems was evaluated separately by analyzing the behavioral responses of strains defective in either motility system, i.e., fla+ laf- (swimming only) or fla- laf+ (swarming only) mutants. capillary ... | 1990 | 2294089 |
| polyvibrio infections: vibrio vulnificus and vibrio parahaemolyticus dual wound and multiple site infections. | | 1990 | 2299220 |
| in vitro activity of norfloxacin and other antibacterial agents against gastro-intestinal pathogens isolated in sweden. | the in vitro activity of norfloxacin was compared to that of ampicillin, doxycycline, chloramphenicol, trimethoprim in combination with sulfamethoxazole (1/20), and erythromycin, against 272 clinical isolates of gastro-intestinal pathogens. norfloxacin was the most active compound of those tested with mics in the range 0.004-2 mg/l. concentrations inhibiting 90% of the strains (mic 90) were 0.008 mg/l for vibrio cholerae, 0.016 mg/l for aeromonas hydrophila, 0.032 mg/l for vibrio cholerae non 01 ... | 1990 | 2302351 |
| duplication and variation of the thermostable direct haemolysin (tdh) gene in vibrio parahaemolyticus. | the relationship between phenotypic variation and nucleotide sequence variation of the gene encoding vibrio parahaemolyticus thermostable direct haemolysin (tdh gene) was examined. strains showing a typical haemolysin-positive phenotype carried two chromosomal gene copies (designated tdh1 and tdh2) while tdh-gene-positive strains showing a weakly positive or negative haemolysin phenotype possessed only a single chromosomal gene copy. both gene copies from a typical haemolysin-positive strain wer ... | 1990 | 2319944 |
| comparative analysis of the hemolysin genes of vibrio cholerae non-01, v. mimicus, and v. hollisae that are similar to the tdh gene of v. parahaemolyticus. | the genes encoding the hemolysins similar to the thermostable direct hemolysin (tdh gene) of vibrio parahaemolyticus were cloned from chromosomes of v. mimicus and v. hollisae. these cloned hemolysin genes and previously cloned tdh genes of v. parahaemolyticus and v. cholerae non-01 were compared by physical mapping and by hybridization with oligodeoxyribonucleotide probes. the nucleotide sequences in the coding regions of all the cloned hemolysin genes were very homologous and had only minor va ... | 1990 | 2323548 |
| cloning and characterization of a gene encoding a new thermostable hemolysin from vibrio parahaemolyticus. | a new thermostable hemolysin (delta-vph) gene was cloned from a kanagawa-negative vibrio parahaemolyticus strain into vector pbr322 in escherichia coli k12. the nucleotide and amino acid sequences had no homology with those of the thermostable direct hemolysin (tdh) which causes the kanagawa phenomenon, and of the thermolabile hemolysin (tlh) of v. parahaemolyticus. the gene was present in all v. parahaemolyticus strains tested and also in one strain of v. damsela. | 1990 | 2323552 |