| automated microbiological detection/identification system. | an automated, computerized system, the automicrobic system, has been developed for the detection, enumeration, and identification of bacteria and yeasts in clinical specimens. the biological basis for the system resides in lyophilized, highly selective and specific media enclosed in wells of a disposable plastic cuvette; introduction of a suitable specimen rehydrates and inoculates the media in the wells. an automated optical system monitors, and the computer interprets, changes in the media, wi ... | 1977 | 334798 |
| [chemotherapy of bile tract infections, with special reference to diffusion of sodium cephalothin into bile (author's transl)]. | | 1977 | 335105 |
| [chemotherapy of septicemia and endocarditis]. | | 1977 | 335685 |
| [methods of using the antibody neutralization test in intestinal coli-infections]. | specificity and sensitivity of the antibody neutralization test intended for detection of the o-antigen of enteropathogenic escherichia were checked under experimental conditions. only 3 strains of the klebsiella genus proved to neutralize the antibodies to the enteropathogenic escherichia of the serological group o20:k84. in the rest of the cases a positive result was obtained only in homologous combinations. in comparative study of the microbial cultures of the infected feces on hard nutrient ... | 1977 | 335737 |
| [study of the role of conditionally pathogenic enterobacteria in acute intestinal diseases]. | conditionally pathogenic enterobacteria were isolated in 34.2% of the patients with acute intestinal diseases (1559 cases examined in all). such representatives of enterobacteria as klebsiella, hafnia, citrobacter of 4, 8, and 22 serological groups. proteus mirabilis of 6, 10, 13, 26, and 28 serological groups, and proteus morganii mostly played the etiological role. in some of the patients conditionally pathogenic microbes only aggravated the main disease, taking part in the development of dysb ... | 1977 | 335739 |
| leukocytic response to inhaled bacteria. | using histologic techniques, we have quantified the amount of infiltration of bronchi and alveoli by polymorphonuclear leukocytes during the 4 hours after an aerosol inoculation of mice with bacteria. although the lungs of animals challenged with staphylococcus aureus differed little from those of animals exposed only to a water aerosol, the lungs of animals exposed to klebsiella pneumoniae or to escherichia coli demonstrated significantly greater polymorphonuclear leukocyte infiltrations in bro ... | 1977 | 335934 |
| [study of the transmissibility of multiple drug resistance and the capacity to ferment lactose in a clinical strain of kl. pneumoniae]. | the donor properties of k. pneumoniae pi 220 with multiple drug resistance were studied. it was shown that the above strain carried 2 plasmids, i.e. r-plasmid ppi 220 controling resistance to ampicillin, streptomycin, tetracycline, chloramphenicol, kanamycin and sulphanylamides and plasmid ppi 221 controlling lactose fermentation. both plasmids can be transfered on conjugation to strain e. coli p678 at a temperture of 28 degrees c at a rate of 10(-5) for ppi 220 and 10(-4) for ppi 221. the drug ... | 1977 | 335955 |
| inhibition of acetylating activity in an enzymatic assay for aminoglycoside antibiotics. | the radioenzymatic technique using aminoglycoside-6'-acetyltransferase is suitable for determining serum concentrations of aminoglycoside antibiotics that have a 6'-amino group. standard curves constructed for each drug in normal human serum are generally satisfactory, but we encountered a pool of human serum (designated in pool) that inhibited the acetylating activity of the assay. standard curves for amikacin, kanamycin, gentamicin, and tobramycin in the acetylating assay were all abnormal wit ... | 1977 | 335965 |
| use of rp4 plasmids carrying bacteriophage mu insertions in nitrogen fixing bacteria klebsiella pneumoniae and rhizobium meliloti. | | 1977 | 336028 |
| the nitrogen fixation cistrons of klebsiella pneumoniae. | | 1977 | 336029 |
| nitrogen fixation (nif) regulatory mutants of klebsiella: determination of the energy cost of n2 fixation in vivo. | | 1977 | 336030 |
| kinetics of nitrogenase of klebsiella pneumoniae. heterotropic interactions between magnesium-adenosine 5'-diphosphate and magnesium-adenosine 5'-triphosphate. | the effects of mgadp and mgatp on the kinetics of a pre-steady-state electron-transfer reaction and on the steady-state kinetics of h2 evulution for nitrogenase proteins of k. pneumoniae were studied. mgadp was a competitive inhibitor of mgatp in the mgatp-induced electron transfer from the fe-protein to the mo-fe-protein. a dissociation constant k'i = 20 micron was determined for mgadp. the release of mgadp or a coupled conformation change in the fe-protein of k.pneumoniae occurred with a rate ... | 1977 | 336036 |
| bacteriology of abscesses of the central nervous system: a multicentre prospective study. | pus from 46 patients with abscesses of the central nervous system (cns) was examined for bacteria; bacteria were found in all patients. streptococci were isolated from 36 patients and most isolates were streptococcus milleri, lancefield group f, ottens and winkler type o iii. staphylococci were isolated from nine patients, organisms of the bacteroides group from 11, proteus spp from seven, klebsiella aerogenes from one, and haemophilus aphrophilus from one. pure cultures predominated over mixed ... | 1977 | 336141 |
| [studies on the pathogenicity of klebsiella for calves]. | | 1977 | 336334 |
| characterization of the isolated transferase subunit of citrate lyase as a coa-transferase. evidence against a covalent enzyme-substrate intermediate. | | 1977 | 336371 |
| acetylene reduction (dinitrogen fixation) by clinical isolates of klebsiella pneumoniae. | freshly isolated clinical strains of klebsiella were tested for the ability to fix dinitrogen by the acetylene reduction assay. ability to detect this trait was markedly affected by cultural conditions. when the test was run at 37 degrees c in the presence of yeast extract (50 mg/liter), only 1.6% of the organisms were diazotrophs, whereas this temperature without yeast extract yielded 12.9% positive cultures. the optimum condition found was 28 degrees c without yeast extract (21.9% positive); t ... | 1977 | 336640 |
| [biochemical typing of klebsiella strains isolated from clinical cases]. | 228 klebsiella strains were isolated from 300 klebsiella suspected materials obtained from hacettepe children's hospital using biochemical methods. 122 strains out of 228 klebsiella isolations were identified as k. aerogenes, 39 as k. ozaenae, 39 as k. edwardsii, 17 as k. pneumoniae, 9 as k. atlanta and 2 strains as k. rhinoscleromatis. we believe that these biochemical typing methods may be used routinely with certainty. | 1977 | 337057 |
| a study of antibiotic therapy in fever of unknown origin in neutropenic cancer patients. | neutropenic cancer patients were given carbenicillin, cephalothin, and gentamicin (ccg) during 51 evaluable episodes of fever of unknown origin. patients in whom fever persisted despite these antibiotics and in whom infection had not been documented were randomized after 3 days either to discontinue antibiotics or to add chloramphenicol or clindamycin to ccg. during 19 episodes (37%) an infection was documented during the first 3 days, and during an additional 12 episodes (24%) there was a respo ... | 1977 | 337094 |
| gram-negative bacillary septic arthritis: clinical, radiographic, therapeutic, and prognostic features. | | 1977 | 337494 |
| [modification of the vaginal flora in nonspecific bacterial fluor using the topical aminoglycoside antibiotic framycetin sulfate]. | | 1977 | 337560 |
| [is congenital hour-glass bladder always a malformation? (author's transl)]. | hour-glass bladder was detected by x-ray investigation in a newborn female infant with delayed postpartum micturition. no signs of difficulty in micturition or urinary tract infection were subsequently detected. control x-ray investigation at the age of 5 1/2 months showed a completely normal-shaped urinary bladder. this finding contrasts with the general opinion that hour-glass bladder is caused by a congenital malformation. | 1977 | 337685 |
| adjuvant action of capsular polysaccharide of klebsiella pneumoniae on antibody response. vi. site of its action. | | 1977 | 337706 |
| [development of a new testmethod for surface disinfection procedures. vi. the influence of testmethods on the effect of disinfectants (author's transl)]. | | 1977 | 337738 |
| penicillins activate autolysins extracted from both escherichia coli and klebsiella pneumoniae envelopes. | ampicillin at a 50-mug/ml concentration effects a doubling of the autolysis rate of cell envelopes isolated from klebsiella pneumoniae mir a12. this antibiotic increases also by two- to threefold the depolymerizing activity of both k. pneumoniae and escherichia coli crude extracts on a labeled peptidoglycan-lipoprotein complex. several other penicillins also activate autolysins. | 1977 | 337892 |
| colonization of the botanical environment by klebsiella isolates of pathogenic origin. | growth, survival, and pathogenicity of klebsiella growing in and on environmental foci were examined. total coliforms present in raw wastes from pulp mills were in excess of 10(5)/ml, and 60 to 80% were klebsiella. fecal coliform counts ranged from 10(1) to 10(5)/ml. klebsiella isolates from industrial effluents and a variety of human and bovine mastitis origins multiplied in pulp waste and commonly exceeded 10(6) cells per ml. pathogenic isolates also multiplied in dilute aqueous extracts of sa ... | 1977 | 337900 |
| bladder instillations with trimethoprim-sulfamethoxazole in the treatment of urinary infection. | 205 paraplegic inpatients with urinary infections were treated with trimethoprim-sulfamethoxazole (tmp-smz) bladder instillations (160 mg tmp 800 mg smz daily for 2 consecutive weeks). 89.3% out of 84 patients with recent vertebral trauma and no history of previous urinary infection (group a), and 68.6% out of 121 patients with paraplegia since more than 2 years, severe chronic infection and various pathological conditions of the urinary tract (group b) were found to be sterile at the end of the ... | 1978 | 338266 |
| gentamicin in the treatment of staphylococcal infections. | the aminoglycoside antibiotic, gentamicin, was used to treat staphylococcal infections in eighty-six patients in an open multicentre trial. most of the infections involved the skin and soft tissue and the lower respiratory tract. staphylococcus aureus was the only organism isolated in seventy-four patients; mixed flora were found in twelve. gentamicin was administered, intramuscularly or intravenously, for 7 to 12 days (mean, 10 days) in a mean dose of 3-27 mg/kg per day. clinical and bacteriolo ... | 1977 | 338394 |
| bladder response to klebsiella infection: a scanning electron microscopy study. | the early response of rat bladder epithelium to klebsiella pneumoniae was examined using scanning electron microscopy. increased bacterial adherence with time after challenge paralleled the occurrence of strand formation and ulceration on the bladder epithelium. the early bladder response to the bacteria consisted of altered epithelial membrane structure, strand formation, and exfoliation of some epithelial cells. these observations indicate that k. pneumoniae causes alterations in the bladder e ... | 1977 | 338544 |
| primary structure of the escherichia coli serotype k42 capsular polysaccharide and its serological identity with the klebsiella k63 polysaccharide. | the escherichia coli k42 capsular polysaccharide consists of leads to 3)-alpha-d-galp-(1 leads to 3)-alpha-d-galuap-(1 leads to 3)-alpha-l-fucp-(1 leads to repeating units. the e. coli k42 and klebsiella k63 antigens are serologically identical. | 1978 | 338590 |
| interaction between the fumarate reductase system of escherichia coli and the nitrogen fixation genes of klebsiella pneumoniae. | for phenotypic expression of nif+kp genes in escherichia coli k-12, the anaerobic electron transport system to fumarate must be functional. the role of the fumarate reduction system is to energize the membrane and thus provide the energy necessary for nitrogen fixation. | 1978 | 338593 |
| klebsiella: taxonomy, nomenclature, and communication. | differences in the delineation of klebsiella spp. in the united kingdom from that in the majority of other countries is discussed. an attempt is made to trace and interpret the reasons for these differences and suggestions are made for a rapid resolution of this problem which hazards communication. | 1977 | 338633 |
| evaluation of amoxicillin therapy in ill children. | ampicillin and amoxicillin were evaluated in 37 ill children. detailed pharmacokinetic studies in 27 of these children demonstrated an advantage in oral absorption of amoxicillin over ampicillin at dosages of both 12.5 and 25 mg/kg per dose. individual variation was great for both drugs. no sequence effect was noted for patients receiving ampicillin before either ampicillin or amoxicillin. amoxicillin was tolerated well by the majority of patients, and the drug was not discontinued because of si ... | 1978 | 338647 |
| a comparison of alternate metabolic strategies for the utilization of d-arabinose. | mutants of klebsiella aerogenes w70 that metabolize the uncommon pentose d-arabinose were isolated. these mutants were found to be either constitutive or indicible by d-arabinose for the synthesis of enzymes in the l-fucose pathway. such mutants could then utilize l-fucose isomerase to convert the structurally similar d-arabinose molecule to d-ribulose. d-ribulose is an intermediate and the inducer of an existing ribitol pathway and could thus be metabolized. in those d-arabinose-positive mutant ... | 1977 | 338926 |
| effect of capsular polysaccharide of klebsiella pneumoniae on the differentiation and functional capacity of macrophages cultured in vitro. | | 1977 | 339038 |
| sensitivity of gram-negative bacilli to the serum bactericidal activity: a marker of the host-parasite relationship in acute and persisting infections. | | 1977 | 339328 |
| [bacteriological studies of the human amniotic fluid in repeated amniocenteses]. | the amniocentesis to gain increasing importance ought to be examined whether an alteration of the bacterial flora of the amniotic fluid was caused by repeated amniocentesis. 128 samples of amniotic fluid selected by abdominal amniocentesis were placed in microbiological cultures. bacteria was isolated in 30 cases, originated predominantly by pollution. under the circumstance of strong sterile conditions the amniocentesis proven even when repeated a diagnostical way of procedure which does not re ... | 1977 | 339605 |
| [urinary and serum o-antibodies in patients with chronic pyelonephritis (author's transl)]. | urine (24-hour-specimen, 100-fold concentrated) and serum samples from adults with chronic pyelonephritis have been investigated for o antibody against the strain isolated from the urine by means of the indirect hemagglutination and indirect immunofluorescence technique. using the hemagglutination technique about one third of the urine showed the presence of o antibody. the highest titer demonstrated was 1:16. o antibody determined by the immunofluorescence technique could be demonstrated in 54. ... | 1977 | 339606 |
| hexokinase and glucose-phosphoenolpyruvate phosphotransferase synthesis in klebsiella aerogenes strains growing in continuous culture. | considerable differences in steady-state hexokinase specific activity were found in 16 n.c.i.b. strains of klebsiella aerogenes grown in identical conditions in glucose-limited chemostats. strains of n.c.i.b. 8258 had no detectable activity, but its glucose-phosphoenolpyruvate phosphotransferase specific activity and that of the other strains were closely similar, and it is concluded that this phosphotransferase activity regulates the overall utilization of glucose, in which hexokinase plays no ... | 1977 | 339911 |
| nitrogenase of klebsiella pneumoniae. distinction between proton-reducing and acetylene-reducing forms of the enzyme: effect of temperature and component protein ratio on substrate-reduction kinetics. | non-linear rates of acetylene reduction and concomitant h2 evolution were observed for the nitrogenase of klebsiella pneumoniae at 10 degrees c. a lag phase of 1-4 min, dependent on the ratio of mo-fe protein to fe protein present, occurred before linear rates of acetylene reduction were achieved. a complementary burst phase for concomitant h2 evolution in the presence of acetylene was also observed. when the proton was the only reducible substrate present, linear rates of h2 evolution were obse ... | 1977 | 339912 |
| [an investigation into the influence of human serum on the in-vitro activity of various cephalosporins (author's transl)]. | cephalosporins are being given more and more frequently empirically as initial therapy, until the bacteriological findings become available. the wide selection of cephalosporins available make the choice of the most suitable one difficult for the clinician. cephazolin, cephradine and cephacetrile were tested against three standard bacteria strains (escherichia coli, staphylococcus aureus, kelebsiella pneumoniae) in normal broth and with the addition of 30% inactivated human serum; the geometric ... | 1977 | 340385 |
| correlation of minimum inhibitory concentration and beta-lactamase activity. | the beta-lactamase activity of 510 recently isolated enterobacteriaceae was investigated with a quantitative photometric test. beta-lactamase could be detected in 55 percent of the enterobacteriaceae. at the same time minimal inhibition concentration (mic) of beta-lactam antibiotics was determined. there was no correlation between mic values and lactamase activity. for correct antibacterial therapy the clinician requires information on the lactamase activity of isolated bacteria in addition to t ... | 1977 | 340386 |
| syntheses and properties of kanamycin c derivatives active against resistant bacteria. | | 1977 | 340436 |
| aminoglycoside inactivating enzymes produced by r plasmids of escherichia coli, citrobacter freundii, klebsiella pneumoniae, proteus vulgaris, providencia stuartii and serratia marcescens. | | 1977 | 340445 |
| a hospital epidemic caused by gentamicin-resistant klebsiella aerogenes. | in the 15 months, february 1976 to april 1977, more than 241 patients became colonized with a strain of klebsiella aerogenes, capsular serotype k2, resistant to most antibiotics. urinary tract infection was the most common clinical manifestation but bacteraemia and, occasionally, infections of other sites were encountered. the main reservoir of the epidemic klebsiella was the gut, urine and skin of colonized patients. gut carriage among staff was very uncommon. the most susceptible patients were ... | 1978 | 340580 |
| the use of a fluorescence typing method in an epidemiological study of klebsiella infection in a london hospital. | the fluorescent antibody technique was used to investigate an epidemic of klebsiella infection in a urological ward and to trace the probable source to a contaminated sink in the treatment room. it was also shown that cross infections by particular capsular types were very common within each ward. certain types of klebsiella occurred in cut flower water but could not be associated with the types infecting the patients. antibiotic resistance patterns within one capsular type were found to vary wh ... | 1978 | 340582 |
| complementation analysis of klebsiella pneumoniae mutants defective in nitrogen fixation. | a series of mutants defective in nitrogen fixation (nif) were isolated in klebsiella pneumoniae strain m5a1. the nif mutations were either located on plasmid prd1 or on the k. pneumoniae chromosome. a total of 37 plasmid mutants and 28 chromosomal mutants were employed in complementation tests using the acetylene reduction technique. most mutants could be assigned to one of seven nif cistrons: nifa, nifb, nifd, nife, niff, nifh, and nifk. complementation analysis of two nif deletion mutants conf ... | 1977 | 340923 |
| linkage map of the nitrogen fixation (nif) genes in klebsiella pneumoniae. | the nif cistrons indentified by complementation analysis in the preceding paper (dixon et al., 1977) were mapped with respect to hisd and to each other other by p1 cotransduction and three-factor reciprocal crosses. the order obrained was hisd nifb nifa (nifl) niff nife nifk nifd nifh. analysis of hisd2-nif cotransduction data by the wu equation (wu, 1966) suggested that the nif genes are divided into two clusters: a his-proximal cluster comprising nifba(l)f and a his-distal group of nifekdh. | 1977 | 340924 |
| skin protothecosis in a patient with renal allograft. | the case history of a 30-year-old male kidney transplant patient who developed cutaneous protothecosis is presented. the lesions, initially located over the forearm and around the site of previous av shunt, consisted of maculopapular areas which opened spontaneously and drained purulent sanguineous material. culutre of drainage grew prototheca wickerhamii. other virulent organisms, ie. klebsiella pneumoniae and pseudomonas aeruginosa, were also grown. the patient died of klebsiella septicemia an ... | 1978 | 341343 |
| percutaneous drainage in the treatment of klebsiella pneumoniae lung abscess. | seven cases of lung abscess involving klebsiella pneumoniae with or without other pathogens presented with gross expansion of the involved lobes or segments and severe clinical illness despite medical treatment. operative management by rib resection and tube drainage was successful in each case, one of which was found to be an infected cryptococcoma. postoperative bronchograms were made in six cases. | 1977 | 341405 |
| [lysogeny of k1. pneumoniae]. | | 1977 | 341494 |
| in vitro susceptibility of selected bacteria to cefaclor. | | 1977 | 341537 |
| [significance of suprapubic puncture of the bladder as a diagnostic method]. | in 650 cases for the purpose of a methodical comparison was examined urine got by paracentesis of the bladder and midstream urine. the most important results were: 1. urine got by paracentesis of the bladder was infected in 30.9%, midstream urine in 51.7%. 2. further 39.4% of the samples of midstream urine were poor in germs; in 2.3% these findings corresponded to an infection. 3. only 69.7% of the kinds of germs and 38.8% of the numbers of germs were identical. 4. 28.4% of the samples of midstr ... | 1977 | 341545 |
| [problems in the determination of pathogens in prostatovesiculitis]. | in patients with prostatovesiculitis the qualitative proof of microorganisms from experiment or ejaculate has only an insignificant diagnostic value on account of the lacking possibility of the recognition of contamination germs, it may be concluded to a prostatitis caused by bacteria, when the number of germs established in the exprimate is unequivocally larger than this one in the middle stream urine. in order to use successfully also the differences of the number of germs of ejaculate and mid ... | 1977 | 341592 |
| clinicopathologic conference: persistent empyema, bacteremia and death. | | 1978 | 341702 |
| transfer of nitrogen fixation genes from a bacterium with the characteristics of both rhizobium and agrobacterium. | strain t1k, reported to be rhizobium trifolii strain t1 carrying the drug resistance plasmid ru-1drd, was able to transfer a cluster of nif+ genes to escherichia coli k-12. additional genetic material, resembling the gal-chla region of e. coli, was also transferred from strain t1k. the segregation pattern of these transferred genes suggested that they were on a plasmid. although strain tik was able to nodulate red and white clover, it also formed very slow-growing galls on tomato stems and share ... | 1978 | 342496 |
| utilization of arginine by klebsiella aerogenes. | klebsiella aerogenes utilized arginine as the sole source of carbon or nitrogen for growth. arginine was degraded to 2-ketoglutarate and not to succinate, since a citrate synthaseless mutant grows on arginine as the only nitrogen source. when glucose was the energy source, all four nitrogen atoms of arginine were utilized. three of them apparently did not pass through ammonia but were transferred by transamination, since a mutant unable to produce glutamate by glutamate synthase or glutamate deh ... | 1978 | 342501 |
| evaluation and application of an improved bacteriocin typing method for klebsiella aerogenes. | a bacteriocin typing method was evaluated using 200 strains of klebsiella aerogenes, 93% of which fell into 11 distinct types. the typing technique was successfully applied to the monitoring and control of hospital cross-infection. | 1978 | 342545 |
| [recent aspects of chemical methods for structural elucidation of polysaccharides and complex carbohydrates (author's transl)]. | | 1977 | 342644 |
| hidradenitis suppurativa of the breast areolae. case report. | a case of hidradenitis suppurativa of the areolae of the breasts is presented. the treatment was excision and split-skin grafting, and this was successful. | 1978 | 343131 |
| protective effect of metronidazole in experimental ulcerative colitis. | administration of carrageenan to guinea pigs produces colonic lesions which are similar to those noted in idiopathic ulcerative colitis of human beings. this model was used to determine fecal flora changes and response to antimicrobial probes during the evolution of carrageenan-induced colitis. the results of fecal flora analysis showed that mean coliform concentrations increased from 10(2.7) to 10(7.4) per g during the initial stages of colonic ulceration. pretreatment of carrageenan recipients ... | 1978 | 344126 |
| evaluation of gastrointestinal microflora in necrotizing enterocolitis. | | 1978 | 344848 |
| characterization and clinical identification of enterobacteriaceae by dna hybridization. | | 1978 | 345354 |
| [coliform bacteria in raw and pasteurized milk]. | studied were 360 samples of raw and 1404 samples of pasteurized milk, collected from three milk centers, for the presence of coliform bacteria. it was found that the coli titer of the raw milk varied from 10(-5) up to 10(-7), depending on the season. the regimes of pasteurization applied kill 100 per cent of the present coli organisms. after the thermic treatment the milk was additionally contaminated with coli forms from the containers and the equipment in dependence on the conditions of washin ... | 1977 | 345606 |
| [experience with a new culture medium for salmonella diagnosis (author's transl)]. | a new medium for the cultivation of salmonella is presented which enables an immediate distinction of proteus bacteria and citrobacter. the time needed for diagnosis is reduced, expenditure on staff and material will be reduced. the "positive" salmonella yield from faeces specimens is increased as compared to leifson agar and ss agar. | 1978 | 345692 |
| survey of resistance to metals and antibiotics in clinical isolates of klebsiella pneumoniae in japan. | clinical isolates of k. pneumoniae (746 isolates) were studied for the resistance to four metals and four drugs. the distribution patterns of their susceptibility to hg, cd and as clearly revealed two peaks, but only a single peak of resistance to pb. it can be seen that resistance was demonstrable in media containing the following concentrations of the metals (micrograms/ml): hgcl2, 10; cdcl2, 400; and na2haso4, 400. in k. pneumoniae, the frequency fo resistance to these concentrations of hg, c ... | 1978 | 345693 |
| homologous and cross-reacting antibodies in the sera of gnotobiotic rats. | | 1978 | 346185 |
| fever and infection in leukemic patients: a study of 494 consecutive patients. | all of the febrile episodes occurring in 494 adults with acute leukemia were reviewed. there were an average of 2.39 febrile episodes per patient and the patients spent 28% of their days in the hospital with fever. sixty-four percent of the febrile episodes were due to infection. the most common types of infection were disseminated infection and pneumonia, which together accounted for 69% of the total episodes of documented infection. the etiologic agent was identified in 73% of the documented ... | 1978 | 346201 |
| radioimmunoassay and radioenzymatic assay of a new aminoglycoside antibiotic, netilmicin. | a radioimmunoassay and a radioenzymatic assay for netilmicin, a new aminoglycoside, were developed in our laboratories to assist in the study of the pharmacology of the drug and to establish values for use in its monitoring. the assays are sensitive, precise, and rapid, giving results that correlate (r = 0.90) with each other and with those of a microbiological assay in which klebsiella pneumoniae is used as the test organism. preliminary pharmacological studies show the drug to have a biologica ... | 1978 | 346263 |
| evolution of ribosomal proteins in enterobacteriaceae. | the evolution of ribosomal proteins of about 70 bacterial strains belonging to the family enterobacteriaceae has been studied by use of previously reported data (s. osawa, t. itoh, and e. otaka, j. bacteriol. 107:168-178, 1971) and those obtained in this paper. the proximity of the bacteria was quantified by co-chromatographing the differentially labeled ribosomal proteins from two strains on a column of carboxymethyl cellulose in various combinations. the were then classified into 12 groups (=s ... | 1978 | 346556 |
| head-space gas liquid chromatography for rapid detection of escherichia coli and proteus mirabilis in urine. | measuring ethanol formed from arabinose by escherichia coli and dimethyl disulphide formed from methionine by proteus mirabilis is a rapid way of detecting these organisms in the urine. ethanol and dimethyl disulphide are detected by head-space gas-liquid chromatography. since sedimented organisms and not whole urine are used the method is not subject to interference from compounds within the urine. when it was tested on 122 samples of urine, 94 from patients with suspected urinary infection and ... | 1978 | 346614 |
| prospective trials of dexamethasone and aerosolized gentamicin in the treatment of inhalation injury in the burned patient. | the addition of an inhalation injury to a cutaneous burn results in a significant increase in patient mortality rates, both from early pulmonary edema and, later, gram-negative pneumonitis. steroids have been shown to decrease mortality in an inhalation injury model. aerosolization of gentamicin has been used successfully to treat severe bronchial infections. therefore, a prospective, randomized trial was undertaken to evaluate both these drugs. sixty burned patients, with inhalation injury conf ... | 1978 | 347099 |
| genetic map of the bacteriocinogenic plasmid clo df13 derived by insertion of the transposon tn901. | an ampicillin transposon tn901 was used as a "mutagen" to isolate insertion mutants of the bacteriocinogenic plasmid clo df13. by combining the obtained heteroduplex and restriction maps of the clo df13::tn901 plasmids (van emboden et al., 1977b) with their polypeptide pattern in minicells, we were able to map five genes on the clo df13 genome. these five genes designated a (cloacin gene), b, c, d, and g cover 55% of the coding capacity of clo df13 dna. since integration of tn901 within these fi ... | 1978 | 347243 |
| factors influencing kanamycin resistance in gram-negative enteric neonatal sepsis. | | 1978 | 347372 |
| common plasmid specifying tobramycin resistance found in two enteric bacteria isolated from burn patients. | tobramycin-resistant burn wound isolates of klebsiella pneumoniae and enterobacter cloacae, together with escherichia coli k-12 transconjugants from these two strains, were examined for plasmid deoxyribonucleic acid (dna). all the resistant strains contained a common, high-molecular-weight, covalently closed circular dna plasmid that was absent in the tobramycin-susceptible e. coli recipient strain. the common plasmid residing in e. cloacae was designated pie098, and that residing in k. pneumoni ... | 1978 | 348100 |
| sequential studies in ankylosing spondylitis. association of klebsiella pneumoniae with active disease. | a study of 163 patients with ankylosing spondylitis seen on 433 occasions showed that active inflammatory disease was strongly associated with the presence of klebsiella pneumoniae in the faeces (p less than 0.001). sequential studies showed that in patients with inactive disease the presence of a positive culture for klebsiella was associated with the subsequent development of active inflammatory disease (p less than 0.001). these findings support the hypothesis that kl. pneumoniae may be an in ... | 1978 | 348130 |
| amikacin therapy of gram-negative bacteremia and meningitis. treatment in diseases due to multiple resistant bacilli. | the therapeutic efficacy of amikacin was evaluated in patients with serious hospital-acquired infections caused by gram-negative bacilli susceptible to amikacin, but usually resistant to kanamycin, gentamicin, and tobramycin. the infections for which amikacin was given were gram-negative bacteremia in 15 patients and gram-negative meningitis in two patients. therapy with amikacin resulted in a cure in 13 patients, improvement in 1, and failure in 3. continuous intravenous infusion of amikacin yi ... | 1978 | 348135 |
| combined action of phosphomycin with streptomycin and gentamicin. | the combined action of phosphomycin with streptomycin and gentamicin against 41 strains of gram-positive micrococci (s. aureus and str. faecalis) and 87 strains of gram-negative bacilli (including 38 strains of pseudomonas) was evaluated. the combination of phosphomycin with streptomycin acted synergistically against 12 strains of gram-positive micrococci, and the combination of phosphomycin with gentmicin against 10 strains. the combination of phosphomycin with streptomycin acted synergisticall ... | 1977 | 348150 |
| action of pseudomonas isoamylase on various branched oligo and poly-saccharides. | pseudomonas isoamylase (ec 3.2.1.68) hydrolyzes (1 linked to 6)-alpha-d-glucosidic linkages of amylopectin, glycogen, and various branched dextrins and oligosaccharides. the detailed structural requirements for the substrate are examined qualitatively and quantitatively in this paper, in comparison with the pullulanase of klebsiella aerogenes. as with pullulanase, ps. isoamylase is unable to cleave d-glucosyl stubs from branched saccharides. ps. isoamylase differs from pullulanase in the followi ... | 1978 | 348322 |
| comparative clinicopathological and bacteriological studies in schistosomiasis mansoni. | fifty male egyptian inpatients with active colonic mansoniasis were studied clinicopathologically, endoscopically, radiologically and bacteriologically. this was done to find out the pattern of colonic and urinary enterobacteria and serum antisalmonella agglutinins in various stages of mansoniasis. cases are divided into three groups (a, b and c) according to the stage of their hepato-splenomegally. their clinical presentations were dysentery, rectal bleeding, abdominal distension, anaemia, endo ... | 1978 | 348448 |
| bacteriology of diarrhoea in egyptian children. | | 1977 | 348549 |
| effect of oxygen supply on growth of klebsiella aerogenes in a multistage tower fermentor. | the effect of the rate of oxygen supply on biomass growth, consumption of carbon source formation of metabolic by-products, biomass yeilds referred to c-source and oxygen, respiration rate and the respiratory quotient was studied in a multistage tower fermentor with an interstage backflow, i.e. with a continuous reinoculation of the preceding stages. experiments were done with klebsiella aerogenes ccm 2318 in a synthetic glucose medium with constant glucose concentration in the feed, at ph 7.0. ... | 1978 | 348585 |
| bowls and bacteria. | an episode of nosocomial infection with klebsiella aerogenes in a surgical ward, in which six patients were infected, is described. the cause of the outbreak was identified as being contaminated washing-bowls. it is recommended that each patient should have his own bowl, which should be disinfected after use, dried and stored upside-down. | 1978 | 349076 |
| cefamandole and cefazolin in the therapy of complicated urinary tract infections. | the clinical efficacy and tolerance of cefamandole, a new cephalosporin antibiotic effective against indole-positive strains of proteus, and cefazolin were studied after intramuscular administration of 500 mg of either of the two cephalosporins every 8 hr for seven days in a prospective, randomized study of 65 elderly male patients with complicated urinary tarct infections. both antibiotics were effective in eradicating the infections, and there was no significant difference between the two grou ... | 1978 | 349091 |
| efficacy and safety of cefamandole plus either gentamicin or tobramycin in therapy of severe gram-negative bacterial infections. | thirty-one patients with severe gram-negative bacterial infections were treated successfully with a combination of cefamandole nafate plus gentamicin or tobramycin. the patients were divided into two treatment groups: group 1 received low-dose therapy (80--100 mg of cefamandole nafate/kg per 24 hr plus 3 mg of either gentamicin or tobramycin/kg per 24 hr), and group 2 patients, who had suspected bacteremia, received high-dose therapy (170 mg of cefamandole nafate/kg per 24 hr plus 5 mg of either ... | 1978 | 349093 |
| antibacterial activity of cefamandole in vitro. | cefamandole, a new cephalosporin derivative, was found to have a broad spectrum of antimicrobial activity against a cross-section of both gram-positive and gram-negative bacteria isolated clinically. gram-positive cocci, except for streptococcus faecalis, were extremely susceptible to cefamandole; penicillin g-resistant staphylococcus aureus also was highly susceptible. minimal bactericidal concentrations for gram-positive cocci approximated the minimal inhibitory concentrations. strains of haem ... | 1978 | 349095 |
| differences between cephalothin and newer parenterally absorbed cephalosporins in vitro: a justification for separate disks. | the activities of cefamandole and cefoxitin in vitro were compared with that of cephalothin against staphylococci and gram-negative bacilli. cephalothin was the most active agent against staphylococci. cefamandole exhibited the greatest activity against the enterobacteriaceae, with the exceptions of serratia marcescens and indole-positive proteeae, against which cefoxitin was the most active antibiotic. the activity of newer cephalosporins that are resistant to the beta-lactamases of gram-negati ... | 1978 | 349096 |
| a comparative study of the activity of cefamandole and other cephalosporins and analysis of the beta-lactamase stability and synergy of cefamandole with aminoglycosides. | the antibacterial activity of cefamandole against 445 clinical isolates was investigated and compared with the activity of other known cephalosporins (cephalothin, cephaloridine, cephalexin, and cefazolin) and of two penicillins (ampicillin and carbenicillin). cefamandole was the most active antibiotic against isolates of citrobacter, enterobacter, and shigella, and its activity against staphylococcus aureus, bacteroides, and some members of the enterobacteriaceae was comparable to that of the o ... | 1978 | 349097 |
| microbial iron-chelators and their action on klebsiella infections in the skin of guinea-pigs. | preparations of catechols from ethyl acetate extracts of cultures of klebsiellae in a low-iron medium contained iron-chelators whose potency was measured by the reversal of the bacteristasis of escherichia coli and klebsiellae in unheated horse serum, and of the growth-inhibition of these two organisms by ethylene diamine di-orthohydroxyphenyl acetic acid (edda). as revealed by in situ tests of paper chromatograms, there was a multiplicity of biologically active chelators in the preparations. ca ... | 1978 | 350255 |
| biologic properties of bacterial lipopolysaccharides treated with chromium chloride. | addition of small amounts of chromium chloride to a saline suspension of salmonella typhosa lipopolysaccharide (lpd; difco) caused a marked reduction in several of the biologic activities of this substance including toxicity, b-cell mitogenicity, plasma colony-stimulating activity (csa), radioprotective effect, and induction of the dermal shwartzman reaction. nevertheless, lps treated with chromium chloride was found to be at least as effective as untreated lps in enhancing resistance of b6cbf1 ... | 1978 | 350361 |
| differential susceptibility of indole-positive and -negative strains of klebsiella pneumoniae to cefazolin, choramphenicol and tetracycline. | biochemical properties and antibiotic susceptibilities of 168 clinical isolates of klebsiella pneumoniae were tested. on the basis of the indole reaction, 30 isolates (18%) were indole-positive and 138 isolates (82%) were indole-negative. a significant difference in antibiotic susceptibility was found in each of the two groups of isolates. of the indole-negative isolates, 82.6% were susceptible to cefazolin at 6.25 microgram/ml or lower, while the indole-positive isolates varied in susceptibilit ... | 1978 | 350514 |
| on the significance of the prosthetic group composition of citrate lyase. | 1. klebsiella aerogenes contains two different acyl carrier proteins, one specific for citrate lyase, the other for fatty acid synthetase. 2. the acyl carrier protein of fatty acid synthetase from k. aerogenes was isolated and compared with the corresponding protein from escherichia coli and with the acyl carrier protein of citrate lyase from k. aerogenes. 3. as judged from prosthetic group compositions as well as amino acid and fingerprint analyses, the acyl carrier proteins of the two fatty ac ... | 1978 | 350581 |
| the incidence, pathogenicity and pathology of bacterial and fungal species in the mare's uterus. | | 1977 | 350737 |
| selective inhibition of klebsiella aerogenes growth on pentoses by pentitols. | selective inhibition of growth by pentitols was observed when klebsiella aerogenes m-7 which could not utilize pentitols was grown on pentoses. d-arabitol inhibited the growth on d-arabinose as a sole carbon source, but had no effect on the growth on l-arabinose, d-xylose, and d-ribose. similarly, l-arabitol inhibited the growth on d-arabinose and l-arabinose, ribitol inhibited the growth on d-arabinose and l-arabinose, and xylitol inhibited the growth on d-xylose. from the following reasons, we ... | 1978 | 350845 |
| bacteriophage mu-induced deletions in a plasmid containing the nif (n2 fixation) genes of klebsiella pneumoniae. | five plasmids with insertions of a heat-inducible mu prophage in a mu-sensitive and p1-sensitive derivative of plasmid prd1, a recombinant r factor containing the his-nif region of klebsiella pneumoniae, were isolated and characterized. in one plasmid containing the mu prophage integrated at the his-distal end of nif, selection for heat resistance resulted in the generation of deletions extending from the mu prophage into the nif region. thirty of these deltions were used to map 26 point mutatio ... | 1978 | 350851 |
| experimental scleroma. a histopathological study. | klebsilla rhinoscleromatis suspension was experimentally inoculated into the nose and maxillary sinus of eight adult healthy rabbits. two normal litter-mates served as controls. the animals were killed at varying intervals up to five months following the inoculation. histopathological examination of the upper and lower respiratory tracts revealed a consistent pulmonary peribronchial and perivascular granulomatous reaction with a mikulic cell-like appearance. the reaction was present in all the i ... | 1978 | 351111 |
| [severe klebsiella serogenes infections]. | it is reported on 2 cases of klebsiella-septikaemia with difficult course. germs of the group klebsiella aerogenes could be proved microbiologically. on the basis of the peculiarities of the clinical course the author adopts a definite attitude to the development of the infection, the pathogenesis, to the microbiological proof and to the aimed antimicrobial chemotherapy. the antibiotic treatment was successful in the first case with karbenicillin and gentamycin and in the second case with cephal ... | 1978 | 351992 |
| transferable antibiotic resistance in enterobacteriaceae isolated from urinary tract infections in general practice. | 138 different coliform isolates from patients with uti in 2 general practices in stockport, england, showed an incidence of drug resistance of 36%: 47% of these strains transferred resistance to e. coli k12. multiple resistance, i.e. resistance to more than 2 drugs occurred in more than a quarter of resistant isolates and sulphonamide resistance was most common. more than twice as many strains in this series (1973--74) were sulphonamide resistant compared with a previous survey (1968--70). mic v ... | 1978 | 352037 |
| new in vitro model to study the effect of antibiotic concentration and rate of elimination on antibacterial activity. | a new apparatus is described which serves to investigate the in vitro antibacterial activity of antibiotics as a function of different concentration time curves. the apparatus can be adjusted to simulate the biexponential serum level curves observed in vivo after oral or intramuscular administration. preliminary studies were carried out with a cephalosporin derivative, cefazolin, against escherichia coli and klebsiella sp. strains simulating initial concentrations of 5, 10, and 20 mug/ml that de ... | 1978 | 352258 |
| amino-acid sequence of citrate-lyase acyl-carrier protein from klebsiella aerogenes. | | 1978 | 352686 |
| reversal of jejunal water secretion by glucose in rats exposed to coliform enterotoxins. | glucose absorption and glucose-facilitated water transport were assessed in rats exposed to semipurified preparations of the heat-labile (lt) and heat-stable (st) enterotoxins of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae by in vivo jejunal perfusion of these toxins alone and with varying amounts of glucose. progressive increases in the glucose concentration of from 12 to 56 mm resulted in incremental rises in water absorption from perfusates containing each of these toxin ... | 1978 | 352789 |