| composition and diversity analysis of the gut bacterial community of the oriental armyworm, mythimna separata, determined by culture-independent and culture-dependent techniques. | the intestinal bacteria community structure and diversity of the oriental armyworm, mythimna separata (walker) (lepidoptera: noctuidae), was studied by analysis of a 16s rdna clone library, denaturing gradient gel electrophoresis,and culture-dependent techniques. the 16s rdna clone library revealed a bacterial community diversity comprising cyanobacteria, firmicutes, actinobacteria, gracilicutes and proteobacteria, among which escherichia coli (migula) (enterobacteriales: enterobacteriaceae) was ... | 2013 | 24773514 |
| microbial degradation of acetamiprid by ochrobactrum sp. d-12 isolated from contaminated soil. | neonicotinoid insecticides are one of the most important commercial insecticides used worldwide. the potential toxicity of the residues present in environment to humans has received considerable attention. in this study, a novel ochrobactrum sp. strain d-12 capable of using acetamiprid as the sole carbon source as well as energy, nitrogen source for growth was isolated and identified from polluted agricultural soil. strain d-12 was able to completely degrade acetamiprid with initial concentratio ... | 2013 | 24386105 |
| comparative clinical study of different multiplex real time pcr strategies for the simultaneous differential diagnosis between extrapulmonary tuberculosis and focal complications of brucellosis. | both brucellosis and tuberculosis are chronic-debilitating systemic granulomatous diseases with a high incidence in many countries in africa, central and south america, the middle east and the indian subcontinent. certain focal complications of brucellosis and extrapulmonary tuberculosis are very difficult to differentiate clinically, biologically and radiologically. as the conventional microbiological methods for the diagnosis of the two diseases have many limitations, as well as being time-con ... | 2013 | 24349599 |
| improving glyphosate oxidation activity of glycine oxidase from bacillus cereus by directed evolution. | glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (go) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the c-n bond in glyphosate. here, in an effort to improve the catalytic activity of the glycine oxidase that was cloned from a glyphosate-degrading marine strain of bacillus cereus (bcego), we used a bacteriophage ... | 2013 | 24223901 |
| central line maintenance bundles and clabsis in ambulatory oncology patients. | pediatric oncology patients are frequently managed with central lines as outpatients, and these lines confer significant morbidity in this immune-compromised population. we aimed to investigate whether a multidisciplinary, central line maintenance care bundle reduces central line-associated bloodstream infections (clabsis) and bacteremias in ambulatory pediatric oncology patients. | 2013 | 24101764 |
| merops: the database of proteolytic enzymes, their substrates and inhibitors. | peptidases, their substrates and inhibitors are of great relevance to biology, medicine and biotechnology. the merops database (http://merops.sanger.ac.uk) aims to fulfill the need for an integrated source of information about these. the database has hierarchical classifications in which homologous sets of peptidases and protein inhibitors are grouped into protein species, which are grouped into families, which are in turn grouped into clans. recent developments include the following. a communit ... | 2013 | 24157837 |
| merops: the database of proteolytic enzymes, their substrates and inhibitors. | peptidases, their substrates and inhibitors are of great relevance to biology, medicine and biotechnology. the merops database (http://merops.sanger.ac.uk) aims to fulfill the need for an integrated source of information about these. the database has hierarchical classifications in which homologous sets of peptidases and protein inhibitors are grouped into protein species, which are grouped into families, which are in turn grouped into clans. recent developments include the following. a communit ... | 2013 | 24157837 |
| monooxygenase, a novel beta-cypermethrin degrading enzyme from streptomyces sp. | the widely used insecticide beta-cypermethrin has become a public concern because of its environmental contamination and toxic effects on mammals. in this study, a novel beta-cypermethrin degrading enzyme designated as cmo was purified to apparent homogeneity from a streptomyces sp. isolate capable of utilizing beta-cypermethrin as a growth substrate. the native enzyme showed a monomeric structure with a molecular mass of 41 kda and pi of 5.4. the enzyme exhibited the maximal activity at ph 7.5 ... | 2013 | 24098697 |
| intravitreal moxifloxacin in the management of ochrobactrum intermedium endophthalmitis due to metallic intraocular foreign body. | a healthy 34-year-old man presented with ochrobactrum intermedium endophthalmitis due to a metallic intraocular foreign body. after vitrectomy, lensectomy, removal of the metallic intraocular foreign body, intravitreal vancomycin and ceftazidime, and systemic ciprofloxacin, intraocular inflammation worsened. repeat vitreous culture confirmed persistent endophthalmitis due to multidrug-resistant o. intermedium. the endophthalmitis successfully resolved after the administration of intravitreal mox ... | 2013 | 24039392 |
| biodegradation of endocrine disruptors in solid-liquid two-phase partitioning systems by enrichment cultures. | naturally occurring and synthetic estrogens and other molecules from industrial sources strongly contribute to the endocrine disruption of urban wastewater. because of the presence of these molecules in low but effective concentrations in wastewaters, these endocrine disruptors (eds) are only partially removed after most wastewater treatments, reflecting the presence of these molecules in rivers in urban areas. the development of a two-phase partitioning bioreactor (tppb) might be an effective s ... | 2013 | 23728808 |
| the impact of nitric oxide toxicity on the evolution of the glutathione transferase superfamily: a proposal for an evolutionary driving force. | glutathione transferases (gsts) are protection enzymes capable of conjugating glutathione (gsh) to toxic compounds. during evolution an important catalytic cysteine residue involved in gsh activation was replaced by serine or, more recently, by tyrosine. the utility of these replacements represents an enigma because they yield no improvements in the affinity toward gsh or in its reactivity. here we show that these changes better protect the cell from nitric oxide (no) insults. in fact the dinitr ... | 2013 | 23828197 |
| the outcome of non-carbapenem-based empirical antibacterial therapy and vre colonisation in patients with hematological malignancies. | febrile neutropenia (fn) is generally a complication of cancer chemotherapy. | 2013 | 24235937 |
| reply to "mistaken identity of brucella infection". | | 2013 | 23682101 |
| impact of hourly emergency department patient volume on blood culture contamination and diagnostic yield. | emergency departments (eds) are an important diagnostic site for outpatients with potentially serious infections. eds frequently experience high patient volumes, and crowding has been shown to negatively impact the delivery of early care for serious infections, such as pneumonia. here, we hypothesized that other important factors in the early care of infectious diseases, the rate of blood culture contamination and the accurate detection of pathogens, would be sensitive to ed operational stress, ... | 2013 | 23515549 |
| draft genome sequence of ochrobactrum pseudogrignonense strain cdb2, a highly efficient arsenate-resistant soil bacterium from arsenic-contaminated cattle dip sites. | we report the 4.97-mb draft genome sequence of a highly efficient arsenate-resistant bacterium, ochrobactrum sp. strain cdb2. it contains a novel arsenic resistance (ars) operon (arsr-arsc1-acr3-arsc2-arsh-mfs) and two non-operon-associated ars genes, arsc3 and arsb. the genome information will aid in the understanding of the arsenic resistance mechanism of this and other bacterial species. | 2013 | 23599296 |
| structural insights into omega-class glutathione transferases: a snapshot of enzyme reduction and identification of a non-catalytic ligandin site. | glutathione transferases (gsts) are dimeric enzymes containing one active-site per monomer. the omega-class gsts (hgsto1-1 and hgsto2-2 in humans) are homodimeric and carry out a range of reactions including the glutathione-dependant reduction of a range of compounds and the reduction of s-(phenacyl)glutathiones to acetophenones. both types of reaction result in the formation of a mixed-disulfide of the enzyme with glutathione through the catalytic cysteine (c32). recycling of the enzyme utilize ... | 2013 | 23593192 |
| clinical characteristics of ochrobactrum anthropi bacteremia. | the clinical picture of ochrobactrum anthropi infection is not well described because the infection is rare in humans and identification of the pathogen is difficult. we present a case of o. anthropi bacteremia that was initially misidentified as ralstonia paucula and later identified by 16s rrna sequencing and reca analysis. | 2013 | 23363833 |
| co-phylog: an assembly-free phylogenomic approach for closely related organisms. | with the advent of high-throughput sequencing technologies, the rapid generation and accumulation of large amounts of sequencing data pose an insurmountable demand for efficient algorithms for constructing whole-genome phylogenies. the existing phylogenomic methods all use assembled sequences, which are often not available owing to the difficulty of assembling short-reads; this obstructs phylogenetic investigations on species without a reference genome. in this report, we present co-phylog, an a ... | 2013 | 23335788 |
| development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five brucella species. | the zoonosis brucellosis causes economically significant reproductive problems in livestock and potentially debilitating disease of humans. although the causative agent, organisms from the genus brucella, can be differentiated into a number of species based on phenotypic characteristics, there are also significant differences in genotype that are concordant with individual species. this paper describes the development of a five target multiplex assay to identify five terrestrial brucella species ... | 2014 | 25495428 |
| unexpected diversity of feral genetically modified oilseed rape (brassica napus l.) despite a cultivation and import ban in switzerland. | despite cultivation and seed import bans of genetically modified (gm) oilseed rape (brassica napus l.), feral gm plants were found growing along railway lines and in port areas at four sites in switzerland in 2011 and 2012. all gm plants were identified as glyphosate-resistant gm event gt73 (roundup ready, monsanto). the most affected sites were the rhine port of basel and the st. johann freight railway station in basel. to assess the distribution and intra- and interspecific outcrossing of gm o ... | 2014 | 25464509 |
| characterization of exoelectrogenic bacteria enterobacter strains isolated from a microbial fuel cell exposed to copper shock load. | microorganisms capable of generating electricity in microbial fuel cells (mfcs) have gained increasing interest. here fourteen exoelectrogenic bacterial strains were isolated from the anodic biofilm in an mfc before and after copper (cu) shock load by hungate roll-tube technique with solid ferric (iii) oxide as an electron acceptor and acetate as an electron donor. phylogenetic analysis of the 16s rrna gene sequences revealed that they were all closely related to enterobacter ludwigii dsm 16688t ... | 2014 | 25412475 |
| kocuria rhizophila misidentified as corynebacterium jeikeium and other errors caused by the vitek ms system call for maintained microbiological competence in the era of matrix-assisted laser desorption ionization-time of flight mass spectrometry. | | 2014 | 25392349 |
| kocuria rhizophila misidentified as corynebacterium jeikeium and other errors caused by the vitek ms system call for maintained microbiological competence in the era of matrix-assisted laser desorption ionization-time of flight mass spectrometry. | | 2014 | 25392349 |
| enterovirus-71 virus-like particles induce the activation and maturation of human monocyte-derived dendritic cells through tlr4 signaling. | enterovirus 71 (ev71) causes seasonal epidemics of hand-foot-and-mouth disease and has a high mortality rate among young children. we recently demonstrated potent induction of the humoral and cell-mediated immune response in monkeys immunized with ev71 virus-like particles (vlps), with a morphology resembling that of infectious ev71 virions but not containing a viral genome, which could potentially be safe as a vaccine for ev71. to elucidate the mechanisms through which ev71 vlps induce cell-med ... | 2014 | 25360749 |
| lessons from mother: long-term impact of antibodies in breast milk on the gut microbiota and intestinal immune system of breastfed offspring. | from birth to adulthood, the gut microbiota matures from a simple community dominated by a few major bacterial groups into a highly diverse ecosystem that provides both benefits and challenges to the host. currently there is great interest in identifying environmental and host factors that shape the development of our gut microbiota. breast milk is a rich source of maternal antibodies, which provide the first source of adaptive immunity in the newborn's intestinal tract. in this addendum, we sum ... | 2014 | 25483336 |
| reduction of hexavalent chromium by the thermophilic methanogen methanothermobacter thermautotrophicus. | despite the significant progress on iron reduction by thermophilic microorganisms, studies on their ability to reduce toxic metals are still limited, despite their common co-existence in high temperature environments (up to 70°c). in this study, methanothermobacter thermautotrophicus, an obligate thermophilic methanogen, was used to reduce hexavalent chromium. experiments were conducted in a growth medium with h2/co2 as substrate with various cr(6+) concentrations (0.2, 0.4, 1, 3, and 5 mm) in t ... | 2014 | 26120143 |
| reduction of hexavalent chromium by the thermophilic methanogen methanothermobacter thermautotrophicus. | despite the significant progress on iron reduction by thermophilic microorganisms, studies on their ability to reduce toxic metals are still limited, despite their common co-existence in high temperature environments (up to 70°c). in this study, methanothermobacter thermautotrophicus, an obligate thermophilic methanogen, was used to reduce hexavalent chromium. experiments were conducted in a growth medium with h2/co2 as substrate with various cr(6+) concentrations (0.2, 0.4, 1, 3, and 5 mm) in t ... | 2014 | 26120143 |
| complete genome assembly of reference strain ochrobactrum anthropi atcc 49687. | ochrobactrum anthropi is an occasional cause of nosocomial infections; however, interest in the organism lies in its phylogenetic proximity to the genus brucella. here, we present the 4.9-mb finished genome of ochrobactrum anthropi atcc 49687, most commonly used as an exclusionary reference organism. | 2014 | 25291772 |
| bloodstream infections in patients with hematological malignancies: which is more fatal - cancer or resistant pathogens? | the primary objective of this study was to report the incidence of bloodstream infections (bsis) and clinically or microbiologically proven bacterial or fungal bsis during neutropenic episodes in patients with hematological malignancies. | 2014 | 25258539 |
| ochrobactrum anthropi bacteremia in a preterm infant with cystic fibrosis. | ochrobactrum anthropi infection in newborn patients is rare, and the treatment is challenging because of its widespread and unpredictable resistance to antimicrobial agents and discrepancies between in vitro susceptibility and in vivo efficacy. we report the clinical and microbiological characteristics of ochrobactrum anthropi bacteremia in a preterm patient. | 2014 | 25242942 |
| colonization by endophytic ochrobactrum anthropi mn1 promotes growth of jerusalem artichoke. | the ochrobactrum anthropi mn1 strain, taxonomically identified using 16s ribosomal dna sequence, was isolated from roots of jerusalem artichoke. its endophytic colonization was investigated microscopically using green fluorescent protein introduced by vector phc60. the strain entered jerusalem artichoke tissues through the root, and was localized in the roots and stems. the plant growth-promoting (pgp) effects of o. anthropi mn1 were assessed in greenhouse as well as field trials with different ... | 2014 | 25073416 |
| a novel function of controlled-release nitrogen fertilizers. | | 2014 | 25047661 |
| anatomical localization of commensal bacteria in immune cell homeostasis and disease. | the mammalian gastrointestinal (gi) tract is colonized by trillions of beneficial commensal bacteria that are essential for promoting normal intestinal physiology. while the majority of commensal bacteria are found in the intestinal lumen, many species have also adapted to colonize different anatomical locations in the intestine, including the surface of intestinal epithelial cells (iecs) and the interior of gut-associated lymphoid tissues. these distinct tissue localization patterns permit uniq ... | 2014 | 24942680 |
| immune response varies with rate of dispersal in invasive cane toads (rhinella marina). | what level of immunocompetence should an animal maintain while undertaking long-distance dispersal? immune function (surveillance and response) might be down-regulated during prolonged physical exertion due to energy depletion, and/or to avoid autoimmune reactions arising from damaged tissue. on the other hand, heightened immune vigilance might be favored if the organism encounters novel pathogens as it enters novel environments. we assessed the links between immune defense and long-distance mov ... | 2014 | 24936876 |
| outbreaks of infections associated with drug diversion by us health care personnel. | to summarize available information about outbreaks of infections stemming from drug diversion in us health care settings and describe recommended protocols and public health actions. | 2014 | 24933292 |
| cooperativity among secretory iga, the polymeric immunoglobulin receptor, and the gut microbiota promotes host-microbial mutualism. | secretory iga (siga) antibodies in the intestinal tract form the first line of antigen-specific immune defense, preventing access of pathogens as well as commensal microbes to the body proper. siga is transported into external secretions by the polymeric immunoglobulin receptor (pigr). evidence is reported here that the gut microbiota regulates production of siga and pigr, which act together to regulate the composition and activity of the microbiota. siga in the intestinal mucus layer helps to m ... | 2014 | 24877874 |
| ochrobactrum anthropi induced retropharyngeal abscess with mediastinal extension complicating airway obstruction: a case report. | retropharyngeal abscess with involvement of mediastinal abscess represents an uncommon complication of upper respiratory tract infections. we report a case presenting with a large retropharyngeal abscess with airway obstruction as the primary presenting symptom. contrast-enhanced ct showed a large retropharyngeal abscess in the neck with extension to the upper and posterior mediastinal spaces. the abscess was surgically excised with 200 cc pus drained from the neck and mediastinal regions. we de ... | 2014 | 26229647 |
| precipitation of phosphate minerals by microorganisms isolated from a fixed-biofilm reactor used for the treatment of domestic wastewater. | the ability of bacteria isolated from a fixed-film bioreactor to precipitate phosphate crystals for the treatment of domestic wastewater in both artificial and natural media was studied. when this was demonstrated in artificial solid media for crystal formation, precipitation took place rapidly, and crystal formation began 3 days after inoculation. the percentage of phosphate-forming bacteria was slightly higher than 75%. twelve major colonies with phosphate precipitation capacity were the domin ... | 2014 | 24699031 |
| diagnostic yield of bronchial washing fluid analysis for hemoptysis in patients with bronchiectasis. | bronchiectasis is the main cause of hemoptysis. when patients with bronchiectasis develop hemoptysis, clinicians often perform bronchoscopy and bronchial washing to obtain samples for microbiological and cytological examinations. bronchial washing fluids were analyzed from patients with bronchiectasis who developed hemoptysis, and the clinical impacts of these analyses were examined. | 2014 | 24719142 |
| secretory antibodies in breast milk promote long-term intestinal homeostasis by regulating the gut microbiota and host gene expression. | maintenance of intestinal homeostasis requires a healthy relationship between the commensal gut microbiota and the host immune system. breast milk supplies the first source of antigen-specific immune protection in the gastrointestinal tract of suckling mammals, in the form of secretory iga (siga). siga is transported across glandular and mucosal epithelial cells into external secretions by the polymeric ig receptor (pigr). here, a breeding scheme with polymeric ig receptor-sufficient and -defici ... | 2014 | 24569806 |
| sloth hair as a novel source of fungi with potent anti-parasitic, anti-cancer and anti-bacterial bioactivity. | the extraordinary biological diversity of tropical forests harbors a rich chemical diversity with enormous potential as a source of novel bioactive compounds. of particular interest are new environments for microbial discovery. sloths--arboreal mammals commonly found in the lowland forests of panama--carry a wide variety of micro- and macro-organisms on their coarse outer hair. here we report for the first time the isolation of diverse and bioactive strains of fungi from sloth hair, and their ta ... | 2014 | 24454729 |
| microbiological testing of pharmaceuticals and cosmetics in egypt. | microbial contamination of pharmaceuticals poses a great problem to the pharmaceutical manufacturing process, especially from a medical as well as an economic point of view. depending upon the product and its intended use, the identification of isolates should not merely be limited to the united states pharmacopeia (usp) indicator organisms. | 2015 | 26653032 |
| metabolic response of clostridium ljungdahlii to oxygen exposure. | clostridium ljungdahlii is an important synthesis gas-fermenting bacterium used in the biofuels industry, and a preliminary investigation showed that it has some tolerance to oxygen when cultured in rich mixotrophic medium. batch cultures not only continue to grow and consume h2, co, and fructose after 8% o2 exposure, but fermentation product analysis revealed an increase in ethanol concentration and decreased acetate concentration compared to non-oxygen-exposed cultures. in this study, the mech ... | 2015 | 26431975 |
| homeostasis and its disruption in the lung microbiome. | the disciplines of physiology and ecology are united by the shared centrality of the concept of homeostasis: the stability of a complex system via internal mechanisms of self-regulation, resilient to external perturbation. in the past decade, these fields of study have been bridged by the discovery of the lung microbiome. the respiratory tract, long considered sterile, is in fact a dynamic ecosystem of microbiota, intimately associated with the host inflammatory response, altered in disease stat ... | 2015 | 26432870 |
| draft genome sequence of ochrobactrum anthropi strain w13p3, a halotolerant polycyclic aromatic hydrocarbon-degrading bacterium. | ochrobactrum anthropi w13p3 was isolated from saline soil contaminated by polycyclic aromatic hydrocarbons (pahs) and could degrade pahs with 5% nacl. we report the 5.3-mb draft genome sequence of this strain, which is helpful for understanding the diversity of ochrobactrum spp. and the mechanism of pah degradation in saline environments. | 2015 | 26227610 |
| the enrichment of an alkaliphilic biofilm consortia capable of the anaerobic degradation of isosaccharinic acid from cellulosic materials incubated within an anthropogenic, hyperalkaline environment. | anthropogenic hyperalkaline sites provide an environment that is analogous to proposed cementitious geological disposal facilities (gdf) for radioactive waste. under anoxic, alkaline conditions cellulosic wastes will hydrolyze to a range of cellulose degradation products (cdp) dominated by isosaccharinic acids (isa). in order to investigate the potential for microbial activity in a cementitious gdf, cellulose samples were incubated in the alkaline (∼ph 12), anaerobic zone of a lime kiln waste si ... | 2015 | 26195600 |
| first isolation and characterization of brucella microti from wild boar. | brucella microti was first isolated from common vole (microtus arvalis) in the czech republic in central europe in 2007. as b. microti is the only brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. the present paper is the first to report the isolation of b. microti from a wild boar (sus scrofa), which is also the first isolation of this bacterial species in hungary. | 2015 | 26163135 |
| alterations of lung microbiota in a mouse model of lps-induced lung injury. | acute lung injury (ali) and the more severe acute respiratory distress syndrome are common responses to a variety of infectious and noninfectious insults. we used a mouse model of ali induced by intratracheal administration of sterile bacterial wall lipopolysaccharide (lps) to investigate the changes in innate lung microbiota and study microbial community reaction to lung inflammation and barrier dysfunction induced by endotoxin insult. one group of c57bl/6j mice received lps via intratracheal i ... | 2015 | 25957290 |
| draft genome sequence of ochrobactrum anthropi strain ml7 isolated from soil samples in vinhphuc province, vietnam. | ochrobactrum species are widespread in the environment and can colonize a wide variety of habitats. here, we describe the sequencing of a new environmental isolate of ochrobactrum anthropi isolated from northern vietnam. | 2015 | 25838481 |
| isolation and characterisation of endophytic nitrogen fixing bacteria in sugarcane. | endophytic nitrogen fixing bacteria were isolated from the leaves, stems and roots of industrial variety (cv. u-thong 3; ut3), wild and chewing sugarcane plants grown for 6 weeks in nitrogen (n)-free sand. eighty nine isolates of endophytic bacteria were obtained on n-free agar. an acetylene reduction assay (ara) detected nitrogenase activity in all 89 isolates. three isolates from the chewing (c2hl2, c7hl1 and c34mr1) sugarcane and one isolate from the industrial sugarcane (ut3r1) varieties wer ... | 2015 | 26868592 |
| improved detection of extended spectrum beta-lactamase (esbl)-producing escherichia coli in input and output samples of german biogas plants by a selective pre-enrichment procedure. | the presence of extended-spectrum beta-lactamase (esbl)-producing escherichia coli was investigated in input (manure from livestock husbandry) and output samples of six german biogas plants in 2012 (one sampling per biogas plant) and two german biogas plants investigated in an annual cycle four times in 2013/2014. esbl-producing escherichia coli were cultured by direct plating on chromagar esbl from input samples in the range of 100 to 104 colony forming units (cfu) per g dry weight but not from ... | 2015 | 25799434 |
| invasive group b streptococcal infection in infants in shenzhen, china. | in this study, we aim to investigate the distribution and antibiotic susceptibility of group b streptococcus (gbs) in infants younger than 90 days in shenzhen, china. | 2015 | 25932259 |
| advances in maize transformation technologies and development of transgenic maize. | maize is the principal grain crop of the world. it is also the crop where genetic engineering has been employed to a great extent to improve its various traits. the ability to transform maize is a crucial step for application of gene technology in maize improvement. there have been constant improvements in the maize transformation technologies over past several years. the choice of genotype and the explant material to initiate transformation and the different types of media to be used in various ... | 2016 | 28111576 |
| advances in maize transformation technologies and development of transgenic maize. | maize is the principal grain crop of the world. it is also the crop where genetic engineering has been employed to a great extent to improve its various traits. the ability to transform maize is a crucial step for application of gene technology in maize improvement. there have been constant improvements in the maize transformation technologies over past several years. the choice of genotype and the explant material to initiate transformation and the different types of media to be used in various ... | 2016 | 28111576 |
| the change of a medically important genus: worldwide occurrence of genetically diverse novel brucella species in exotic frogs. | the genus brucella comprises various species of both veterinary and human medical importance. all species are genetically highly related to each other, sharing intra-species average nucleotide identities (ani) of > 99%. infections occur among various warm-blooded animal species, marine mammals, and humans. until recently, amphibians had not been recognized as a host for brucella. in this study, however, we show that novel brucella species are distributed among exotic frogs worldwide. comparative ... | 2016 | 28036367 |
| biochemical and structural analysis of a novel esterase from caulobacter crescentus related to penicillin-binding protein (pbp). | considering that the prevalence of antibiotic-resistant pathogenic bacteria is largely increasing, a thorough understanding of penicillin-binding proteins (pbps) is of great importance and crucial significance because this enzyme family is a main target of β-lactam-based antibiotics. in this work, combining biochemical and structural analysis, we present new findings that provide novel insights into pbps. here, a novel pbp homologue (ccesta) from caulobacter crescentus cb15 was characterized usi ... | 2016 | 27905486 |
| genomic comparison of agrobacterium pusense strains isolated from bean nodules. | | 2016 | 27833604 |
| ochromobactrum intermedium: an emerging opportunistic pathogen-case of recurrent bacteraemia associated with infective endocarditis in a haemodialysis patient. | we describe the first clinical case report of infective endocarditis related to ochrobactrum intermedium infection. the case involved a 23-year-old man receiving dialysis via an internal jugular long-term haemodialysis catheter. he improved with a prolonged course of meropenem and minocycline. ochrobactrum spp. are recognized as rare emerging opportunistic pathogens. | 2016 | 27843545 |
| ochromobactrum intermedium: an emerging opportunistic pathogen-case of recurrent bacteraemia associated with infective endocarditis in a haemodialysis patient. | we describe the first clinical case report of infective endocarditis related to ochrobactrum intermedium infection. the case involved a 23-year-old man receiving dialysis via an internal jugular long-term haemodialysis catheter. he improved with a prolonged course of meropenem and minocycline. ochrobactrum spp. are recognized as rare emerging opportunistic pathogens. | 2016 | 27843545 |
| ochrobactrum anthropi keratitis with focal descemet's membrane detachment and intracorneal hypopyon. | purpose. to describe a unique case of o. anthropi keratitis associated with a rare manifestation of descemet's membrane detachment and intracorneal hypopyon and to discuss challenges in diagnosis and management. methods. best-corrected visual acuity was measured with snellen letters. corneal scrapings were performed and aerobic, viral, herpetic, acid-fast bacilli, acanthamoeba, and fungal stains and cultures were obtained. following evisceration, tissue was evaluated for histologic features and ... | 2016 | 27777806 |
| identification and characterization of cefotaxime resistant bacteria in beef cattle. | third-generation cephalosporins are an important class of antibiotics that are widely used in treatment of serious gram-negative bacterial infections. in this study, we report the isolation of bacteria resistant to the third-generation cephalosporin cefotaxime from cattle with no previous cefotaxime antibiotic exposure. the prevalence of cefotaxime-resistant bacteria was examined by a combination of culture based and molecular typing methods in beef cattle (n = 1341) from 8 herds located in nort ... | 2016 | 27642751 |
| gst activity and membrane lipid saturation prevents mesotrione-induced cellular damage in pantoea ananatis. | callisto(®), containing the active ingredient mesotrione (2-[4-methylsulfonyl-2-nitrobenzoyl]1,3-cyclohenanedione), is a selective herbicide that controls weeds in corn crops and is a potential environmental contaminant. the objective of this work was to evaluate enzymatic and structural changes in pantoea ananatis, a strain isolated from water, in response to exposure to this herbicide. despite degradation of mesotrione, probably due a glutathione-s-transferase (gst) pathway in pantoea ananatis ... | 2016 | 27620734 |
| utility of ochrobactrum anthropi yc152 in a microbial fuel cell as an early warning device for hexavalent chromium determination. | fast hexavalent chromium (cr(vi)) determination is important for environmental risk and health-related considerations. we used a microbial fuel cell-based biosensor inoculated with a facultatively anaerobic, cr(vi)-reducing, and exoelectrogenic ochrobactrum anthropi yc152 to determine the cr(vi) concentration in water. the results indicated that o. anthropi yc152 exhibited high adaptability to ph, temperature, salinity, and water quality under anaerobic conditions. the stable performance of the ... | 2016 | 27537887 |
| relationship between intrauterine bacterial infection and early embryonic developmental arrest. | early embryonic developmental arrest is the most commonly understudied adverse outcome of pregnancy. the relevance of intrauterine infection to spontaneous embryonic death is rarely studied and remains unclear. this study aimed to investigate the relationship between intrauterine bacterial infection and early embryonic developmental arrest. | 2016 | 27270541 |
| draft genome sequence of organophosphate-degrading ochrobactrum anthropi fraf13. | italic! ochrobactrum anthropifraf13 was isolated from farmland soil in jersey village, texas. fraf13 is a bacterial microorganism with broad antibiotic resistance that possesses a number of metal-dependent β-lactam enzymes with secondary phosphotriesterase activity that can initiate the breakdown of organophosphate compounds. | 2016 | 27103720 |
| first case report of human infection with ochrobactrum tritici causing bacteremia and cholecystitis. | | 2016 | 26915622 |
| metagenomic sequencing of the chronic obstructive pulmonary disease upper bronchial tract microbiome reveals functional changes associated with disease severity. | chronic obstructive pulmonary disease (copd) is a major source of mortality and morbidity worldwide. the microbiome associated with this disease may be an important component of the disease, though studies to date have been based on sequencing of the 16s rrna gene, and have revealed unequivocal results. here, we employed metagenomic sequencing of the upper bronchial tract (ubt) microbiome to allow for greater elucidation of its taxonomic composition, and revealing functional changes associated w ... | 2016 | 26872143 |
| the staphylococcus aureus methicillin resistance factor fmta is a d-amino esterase that acts on teichoic acids. | the methicillin resistance factor encoded by fmta is a core member of the staphylococcus aureus cell wall stimulon, but its function has remained elusive for the past two decades. first identified as a factor that affects methicillin resistance in s. aureus strains, fmta was later shown to interact with teichoic acids and to localize to the cell division septum. we have made a breakthrough in understanding fmta function. we show that fmta hydrolyzes the ester bond between d-ala and the backbone ... | 2016 | 26861022 |
| consistent 1,3-propanediol production from glycerol in mixed culture fermentation over a wide range of ph. | glycerol is currently an over-produced chemical that can be used as substrate for the production of high value products such as 1,3-propanediol (1,3-pdo) in fermentation processes. the aim of this study was to investigate the effect of initial ph on a batch mixed culture fermentation of glycerol, considering both the bacterial community composition and the fermentation patterns. | 2016 | 26855671 |
| structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (osdhar) from oryza sativa l. japonica. | dehydroascorbate reductase (dhar) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (gsh)-dependent reduction of oxidized ascorbate (dehydroascorbate, dha). as a result, dhar regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ros). in previous experiments involving transgenic rice, we observed that overexpression of dhar enhanced grain yield and biomass. since the structure of dhar is not available, the enzymatic mechanism is not ... | 2016 | 26775680 |
| a novel naturally occurring class i 5-enolpyruvylshikimate-3-phosphate synthase from janibacter sp. confers high glyphosate tolerance to rice. | as glyphosate is a broad spectrum herbicide extensively used in agriculture worldwide, identification of new aroa genes with high level of glyphosate tolerance is essential for the development and breeding of transgenic glyphosate-tolerant crops. in this study, an aroa gene was cloned from a janibacter sp. strain isolated from marine sediment (designated as aroaj. sp). the purified aroaj. sp enzyme has a km value of 30 μm for pep and 83 μm for s3p, and a significantly higher ki value for glyphos ... | 2016 | 26754957 |
| genome sequence of ochrobactrum anthropi strain subg007, a plant pathogen and potential xenobiotic compounds degradation bacterium. | ochrobactrum anthropi subg007 was isolated from the fruit of prunus dulcis in rajkot (22.30°n, 70.78°e), gujarat, india. here we present the 4.37 mb genome sequence strain subg007, which may provide the genetic information for the application in environment pollution degradation and agriculture field. the strain also posses many genes cluster which involved in production of important secondary metabolites. the nucleotide sequence of this genome was deposited into ncbi genbank under the accession ... | 2017 | 28119820 |
| improvement of whole-cell transamination with saccharomyces cerevisiae using metabolic engineering and cell pre-adaptation. | whole-cell biocatalysis based on metabolically active baker's yeast with engineered transamination activity can be used to generate molecules carrying a chiral amine moiety. a prerequisite is though to express efficient ω-transaminases and to reach sufficient intracellular precursor levels. | 2017 | 28049528 |
| quality control testing for tracking endotoxin-producing gram-negative bacteria during the preparation of polyvalent snake antivenom immunoglobulin. | snake bites represent a serious public health problem, particularly in rural areas worldwide. antitoxic sera preparations are antibodies from immunized animals and are considered to be the only treatment option. the purification of antivenom antibodies should aim at obtaining products of consistent quality, safety, efficacy, and adherence to good manufacturing practice principles. endotoxins are an integral component of the outer cell surface of gram-negative bacteria. they are common contaminat ... | 2017 | 26242786 |
| bacterial strains isolated from pcb-contaminated sediments and their use for bioaugmentation strategy in microcosms. | this study was focused on the characterization of 15 bacterial strains isolated from long-term pcb-contaminated sediment located at the strážsky canal in eastern part of slovakia, in the surroundings of a former pcb producer. pcb-degrading strains were isolated and identified as microbacterium oleivorans, stenotrophomonas maltophilia, brevibacterium sp., ochrobactrum anthropi, pseudomonas mandelii, rhodococcus sp., achromobacter xylosoxidans, stenotrophomonas sp., ochrobactrum sp., pseudomonas a ... | 2014 | 23553615 |
| a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty. | to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka). | 2017 | 27919672 |
| a case of unusual gram-negative bacilli septic arthritis in an immunocompetent patient. | the gram-negative bacilli acinetobacter baumannii, burkholderia cepacia, ochrobactrum anthropi, pseudomonas mendocina, ralstonia spp., serratia marcescens and stenotrophomonas maltophilia are ubiquitous environmental organisms of low virulence, and do not usually cause illness in immunocompetent hosts. we report a case of multiple concurrent opportunistic gram-negative bacilli causing septic arthritis in a healthy patient following trauma to the knee. repeated operations, including arthroscopy, ... | 2013 | 24005465 |
| biodegradation of benzo[a]pyrene by bacterial consortium isolated from mangrove sediment. | benzo[a]pyrene is a high-molecular-weight polycyclic aromatic hydrocarbon highly recalcitrant in nature and thus harms the ecosystem and/or human health. therefore, its removal from the marine environment is crucial. this research focuses on benzo[a]pyrene degradation by using enriched bacterial isolates in consortium under saline conditions. bacterial isolates capable of using benzo[a]pyrene as sole source of carbon and energy were isolated from enriched mangrove sediment. these isolates were i ... | 2017 | 28281885 |
| selected emerging diseases of amphibia. | this review summarizes the most recent updates on emerging infectious diseases of amphibia. a brief summary of batrachochytrium dendrobatidis history, epidemiology, pathogenesis, life cycle, diagnosis, treatment, and biosecurity is provided. ambystoma tigrinum virus, common midwife toad virus, frog virus 3, rana grylio virus, rana catesbeiana ranavirus, mahaffey road virus, rana esculenta virus, bohle iridovirus, and tiger frog virus ranaviruses are extensively reviewed. emerging bacterial patho ... | 2013 | 23642863 |
| isolation, fingerprinting and genetic identification of indigenous pahs degrading bacteria from oil-polluted soils. | in the present study, thirty five bacterial isolates were obtained from hydrocarbon-contaminated soil samples using an enrichment method. these isolates were tested to grow on mineral salt medium containing anthracene or phenanthrene as sole carbon source. only five isolates showed the ability to degrade these compounds. rapd-pcr fingerprinting was carried out for the five isolates, and the dna patterns revealed that there was no similarity among the examined bacteria whenever the rflp using fou ... | 2016 | 26930863 |
| bacterial community analysis of cypermethrin enrichment cultures and bioremediation of cypermethrin contaminated soils. | cypermethrin is widely used for insect control; however, its toxicity toward aquatic life requires its complete removal from contaminated areas where the natural degradation ability of microbes can be utilized. agricultural soil with extensive history of cm application was used to prepare enrichment cultures using cypermethrin as sole carbon source for isolation of cypermethrin degrading bacteria and bacterial community analysis using pcr-dgge of 16 s rrna gene. dgge analysis revealed that domin ... | 2015 | 25656248 |
| 16s ribosomal dna sequence-based identification of bacteria in laboratory rodents: a practical approach in laboratory animal bacteriology diagnostics. | correct identification of bacteria is crucial for the management of rodent colonies. some bacteria are difficult to identify phenotypically outside reference laboratories. in this study, we evaluated the utility of 16s ribosomal dna (rdna) sequencing as a means of identifying a collection of 30 isolates of rodent origin which are conventionally difficult to identify. sequence analysis of the first approximate 720 to 880 bp of the 5'- end of 16s rdna identified 25 isolates (83.33%) with ≥ 99% sim ... | 2014 | 24876090 |
| isolation of a novel 'atypical' brucella strain from a bluespotted ribbontail ray (taeniura lymma). | a pleomorphic gram-negative, motile coccobacillus was isolated from the gills of a wild-caught bluespotted ribbontail ray after its sudden death during quarantine. strain 141012304 was observed to grow aerobically, to be clearly positive for cytochrome oxidase, catalase, urease and was initially identified as "brucella melitensis" or "ochrobactrum anthropi" by matrix-assisted laser desorption/ionization-time of flight mass spectrometry and vitek2-compact(®), respectively. affiliation to the genu ... | 2017 | 27785661 |
| [undulant fever and autoimmune hemolytic anemia in a 20-year-old exchange student from jordan - the human brucellosis as an important differential diagnosis in migrants]. | a 20-year-old jordanian exchange student presents with recurrent fever, night sweats, cough, and swelling and redness around the ankle. physical examination further reveals bilateral ankle arthritis and painful cervical lymphadenopathy. | 2015 | 26625239 |
| brucella suis bacteremia misidentified as ochrobactrum anthropi by the vitek 2 system. | ochrobactrum and brucella are genetically related genera of the family brucellaceae, sharing 98.8% rrna similarity. because of their phenotypic similarity, ochrobactrum can be miscoded as brucella by automated identification systems. the misidentification on blood cultures (bcs) of b. suis as o. anthropi by the vitek 2 system is herein described. a 67-year-old male with a prosthetic mitral valve and fever was admitted with bacteremia due to a gram-negative coccobacillus identified as o. anthropi ... | 2016 | 27131010 |
| infectious endophthalmitis after boston type 1 keratoprosthesis implantation. | to determine the incidence, clinical features, and outcomes of infectious endophthalmitis after boston type 1 keratoprosthesis (kpro) implantation. | 2012 | 22314825 |
| biodegradation of used engine oil by novel strains of ochrobactrum anthropi hm-1 and citrobacter freundii hm-2 isolated from oil-contaminated soil. | used engine oil (ueo) constitutes a serious environmental problem due to the difficulty of disposal off or reuse. ten bacterial strains with biodegradation potential were isolated from ueo-contaminated soil sample using enrichment technique. two strains which exhibited the highest degradation %, 51 ± 1.2 and 48 ± 1.5, respectively, were selected. based on the morphological, biochemical characteristics and 16s rrna sequence analysis, they were identified as ochrobactrum anthropi hm-1 (accession n ... | 2016 | 28330298 |
| microbial metabolism and community structure in response to bioelectrochemically enhanced remediation of petroleum hydrocarbon-contaminated soil. | this study demonstrates that electrodes in a bioelectrochemical system (bes) can potentially serve as a nonexhaustible electron acceptor for in situ bioremediation of hydrocarbon contaminated soil. the deployment of bes not only eliminates aeration or supplement of electron acceptors as in contemporary bioremediation but also significantly shortens the remediation period and produces sustainable electricity. more interestingly, the study reveals that microbial metabolism and community structure ... | 2014 | 24628095 |
| in silico identification for α-amino-ε-caprolactam racemases by using information on the structure and function relationship. | in silico identification for enzymes having desired functions is attractive because there is a possibility that numerous desirable enzymes have been deposited in databases. in this study, α-amino-ε-caprolactam (acl) racemases were searched from the ncbi protein database. four hundred thirteen fold-type i pyridoxal 5'-phosphate-dependent enzymes which are considered to contain sequences of acl racemase were firstly obtained by submitting the sequence of acl racemase from achromobacter obae to the ... | 2015 | 26206345 |
| molecular cloning, purification and biochemical characterization of a novel pyrethroid-hydrolyzing carboxylesterase gene from ochrobactrum anthropi yz-1. | strain yz-1 was isolated from activated sludge and identified as ochrobactrum anthropi. this strain was capable of degrading pyrethroids pesticides, suggesting the presence of degrading enzymes. in the present study, a novel esterase gene pytz was cloned from the genomic library of yz-1 successfully. the pytz contained an open reading frame of 606bp encoding a pyrethroid-hydrolyzing carboxylesterase. deduced amino acid sequence showed moderate identities (39-59%) with most homologous carboxylest ... | 2012 | 22579404 |
| brucella spp. of amphibians comprise genomically diverse motile strains competent for replication in macrophages and survival in mammalian hosts. | twenty-one small gram-negative motile coccobacilli were isolated from 15 systemically diseased african bullfrogs (pyxicephalus edulis), and were initially identified as ochrobactrum anthropi by standard microbiological identification systems. phylogenetic reconstructions using combined molecular analyses and comparative whole genome analysis of the most diverse of the bullfrog strains verified affiliation with the genus brucella and placed the isolates in a cluster containing b. inopinata and th ... | 2017 | 28300153 |
| organophosphonates utilization by soil strains of ochrobactrum anthropi and achromobacter sp. | four bacterial strains from glyphosate- or alkylphosphonates-contaminated soils were tested for ability to utilize different organophosphonates. all studied strains readily utilized methylphosphonic acid and a number of other phosphonates, but differed in their ability to degrade glyphosate. only strains ochrobactrum anthropi gpk 3 and achromobacter sp. kg 16 utilized this compound after isolation from enrichment cultures with glyphosate. achromobacter sp. mpk 7 from the same enrichment culture, ... | 2017 | 28184965 |
| characterization of crude oil degrading bacteria isolated from contaminated soils surrounding gas stations. | a total of twenty bacterial cultures were isolated from hydrocarbon contaminated soil. of the 20 isolates, ram03, ram06, ram13, and ram17 were specifically chosen based on their relatively higher growth on salt medium amended with 4 % crude oil, emulsion index, surface tension, and degradation percentage. these bacterial cultures had 16s rrna gene sequences that were most similar to ochrobactrum cytisi (ram03), ochrobactrum anthropi (ram06 and ram17), and sinorhizobium meliloti (ram13) with 96 % ... | 2016 | 27655077 |
| temperature-dependent regulation of the ochrobactrum anthropi proteome. | ochrobactrum anthropi is a gram-negative rod belonging to the brucellaceae family, able to colonize a variety of environments, and actually reported as a human opportunistic pathogen. despite its low virulence, the bacterium causes a growing number of hospital-acquired infections mainly, but not exclusively, in immunocompromised patients. the aim of this study was to obtain an overview of the global proteome changes occurring in o. anthropi in response to different growth temperatures, in order ... | 2016 | 27753207 |
| a case of ochrobactrum anthropi-induced septic shock and infective endocarditis. | ochrobactrum anthropi is a gram-negative rod of low virulence. infections due to this organism are uncommon; however in immunocompromised hosts it can cause severe infections. among the many infections it can cause, infective endocarditis is very rare. even rarer is infective endocarditis of the native valves, as ochrobactrum antropi affects damaged or prosthetic valves almost exclusively. this case describes native valve endocarditis due to ochrobactrum anthropi. [full article available at http ... | 2016 | 27379356 |
| isolation of bdellovibrio bacteriovorus from a tropical wastewater treatment plant and predation of mixed species biofilms assembled by the native community members. | it is reported here that a predatory bacterium belonging to the genus bdellovibrio, was isolated from activated sludge at the ulu pandan water reclamation plant, singapore. 16s rdna gene sequencing analysis revealed that this isolate was 99% identical to 'bdellovibrio bacteriovorus strain tiberius' and hence is designated as 'bdellovibrio bacteriovorus up'. using a novel approach based on fluorescence in situ hybridization (fish), a prey cell density-dependent growth pattern of b. bacteriovorus ... | 2016 | 27328268 |
| [research progress of ochrobactrum anthropic--a review]. | ochrobactrum anthropi is a gram-negative bacillus, usually known as an opportunistic pathogen. mostly its infection is related with systemic or local lower immunity, and manifested as bacteremia, meningitis, purulent infection. recently, along with expanded infection, it has become an important human pathogen. the prevention, clinical diagnose and treatment become complicated because varied clinical symptoms increased antibiotic resistance and cross immune reaction with others pathogens. in this ... | 2015 | 26665594 |
| ochrobactrum endophyticum sp. nov., isolated from roots of glycyrrhiza uralensis. | a novel gram-staining negative, motile, rod-shaped and aerobic bacterial strain, designated egi 60010(t), was isolated from healthy roots of glycyrrhiza uralensis f. collected from yili county, xinjiang province, north-west china. the 16s rrna gene sequence of strain egi 60010(t) showed 97.2 % sequence similarities with ochrobactrum anthropi atcc 49188(t) and ochrobactrum cytisi esc1(t), and 97.1 % with ochrobactrum lupini lup21(t). the phylogenetic analysis based on 16s rrna gene sequences show ... | 2016 | 26615404 |
| development of a loop-mediated isothermal amplification method for the rapid detection of the dioxin-degrading bacterium ochrobactrum anthropi in soil. | in this study, loop-mediated isothermal amplification (lamp) and real-time lamp assays were developed to detect the dioxin-degrading bacterium ochrobactrum anthropi strain bd-1 in soil. four primers were designed to use its gene amplification for the strain o. anthropi bd-1. the real-time lamp assay was found to accomplish the reaction by 1 pg of genomic dna load when used for nucleic acid amplification. this assay was then applied to detect o. anthropi bd-1 in eight soil samples collected from ... | 2015 | 26144562 |
| evaluation of the removal of pyrene and fluoranthene by ochrobactrum anthropi, fusarium sp. and their coculture. | fluoranthene and pyrene are polycyclic aromatic hydrocarbons of high molecular weight that are recalcitrant and toxic to humans; therefore, their removal from the environment is crucial. from hydrocarbon-contaminated soil, 25 bacteria and 12 filamentous fungi capable of growth on pyrene and fluoranthene as the sole carbon and energy source were isolated. from these isolates, ochrobactrum anthropi bpyf3 and fusarium sp. fpyf1 were selected and identified because they grew quickly and abundantly i ... | 2015 | 25369894 |