| accumulation of a precursor of subunit s1 of pertussis toxin in cell envelopes of bordetella pertussis in response to the membrane perturbant phenethyl alcohol. | exponential cultures of bordetella pertussis strain 18334 were treated with the membrane-perturbing agent phenethyl alcohol which, at a concentration of 0.075% v/v, blocked the synthesis of mature subunit s1 of pertussis toxin as revealed by western blotting. it also caused the accumulation of a precursor, ps1, with an estimated mol. wt of 32 x 10(3), that was located in the cytoplasmic membrane. these findings suggested that subunit s1 of pertussis toxin was exported in a signal peptide-depende ... | 1987 | 2438411 |
| systemic anaphylaxis in mast-cell-deficient mice of w/wv and sl/sld genotypes. | active systemic anaphylaxis was induced in mast-cell-deficient mice of w/wv and sl/sld genotypes. the mast-cell-deficient mice were successfully sensitized either by an intraperitoneal injection of chicken gamma-globulin (c gamma g) mixed with adjuvant, alum and saline extract of bordetella pertussis, or by an intravenous injection of c gamma g alone. sensitized mice showed signs of systemic anaphylaxis and died after one or more intravenous challenge injections of c gamma g. these studies show ... | 1987 | 2440738 |
| monoclonal antibodies to the large glycoproteins of respiratory syncytial virus: possible evidence for several functional antigenic sites. | employing different routes of immunization and different adjuvants, a large number of hybridomas were produced that secreted monoclonal antibodies (mabs) against the large envelope glycoprotein (gp90) and the fusion glycoprotein (gp70) of respiratory syncytial virus (rsv). the antigenic specificity of the mabs was established by immunoblot analysis and radioimmunoprecipitation. three hybridomas secreting anti-gp90 mabs were established from mice immunized by intraperitoneal injection of purified ... | 1987 | 2440982 |
| differential susceptibility to actively induced experimental allergic encephalomyelitis and experimental allergic orchitis among balb/c substrains. | experimental allergic orchitis (eao) and experimental allergic encephalomyelitis (eae) are animal models of organ-specific autoimmune disease. in this study, balb/cbyj and balb/canncr mice were susceptible to both autoimmune diseases whereas balb/cj subline mice were resistant. disease resistance in balb/cj mice did not appear to be a reflection of either (i) a nonspecific generalized impairment of cellular immunity or (ii) an alteration in the phenotypic expression of bordetella pertussis-induc ... | 1987 | 2446778 |
| purified 60-kilodalton legionella protein antigen with legionella-specific and nonspecific epitopes. | in a previous study, all convalescent-phase sera from patients with culture-confirmed legionellosis reacted on immunoblots with a legionella genus-wide 58-kilodalton (kda) protein antigen (j.s. sampson, b.b. plikaytis, and h.w. wilkinson, j. clin. microbiol. 23:92-99, 1986). the present study was done to immunologically characterize and determine the diagnostic relevance of this purified antigen. the antigen was precipitated from enriched cell extracts with ammonium sulfate and purified by high- ... | 1987 | 2447117 |
| human t lymphocyte clones specific for "bordetella pertussis". | | 1986 | 2447923 |
| use of synthetic peptides to map antigenic sites of bordetella pertussis toxin subunit s1. | we synthesized six peptides (15-22 amino acids) from the amino acid sequence of the s1 subunit of pertussis toxin. the antigenicity of the polypeptides was investigated by enzyme-linked immunosorbent assays, in which the polypeptides coupled to bovine serum albumin were used as coating antigens. the polypeptides were examined as antigens for reaction with pre- and postvaccination sera from infants receiving either a bordetella pertussis whole-cell vaccine or a vaccine containing pertussis toxoid ... | 1988 | 2450153 |
| immunological relatedness between bordetella pertussis and rat brain adenylyl cyclases. | a prokaryotic adenylyl cyclase, secreted by bordetella pertussis, shares a common functional property with eukaryotic adenylyl cyclases, i.e., regulation by the eukaryotic protein calmodulin. making use of polyclonal antibodies raised against the bacterial adenylyl cyclase and the rat brain adenylyl cyclase catalytic component, respectively, we showed an immunological cross-reactivity between the two enzymes. furthermore, b. pertussis adenylyl cyclase was inhibited and immunoprecipitated by the ... | 1988 | 2450573 |
| [development of a method for the quantitative determination of bordetella pertussis toxin and the prospects of its use for diagnosis]. | two variants of the enzyme immunoassay (eia) systems for the determination of b. pertussis toxin (bpt), the "double sandwich" system and the competitive assay system, have been developed. for the titration of bpt in b. pertussis antigens the use of fetuin as the affinity base is preferable, and not antibodies from different paired animals. of the two variants, the competitive eia is more promising for diagnostic purposes. | 1988 | 2452534 |
| cellular distribution and biochemical characterization of g proteins in skeletal muscle: comparative location with voltage-dependent calcium channels. | gtp binding proteins have been proposed to play a role in excitation--contraction coupling. in a precedent study [toutant et al., (1988), biochem. j., 405-409], we determined that bordetella pertussis toxin is able to catalyse adp-ribosylation of two substrates in the detergent soluble fraction of total muscle extracts. purified fractions of transverse tubule membranes (t-tubule membranes), a key element of the excitation--contraction coupling, were shown to exhibit a major adp-ribosylated subst ... | 1990 | 2154370 |
| simultaneous determination of bordetella pertussis toxin and filamentous haemagglutinin concentrations by hydroxyapatite high-performance liquid chromatography. | a simple and rapid method for the simultaneous determination of bordetella pertussis toxin (pt) and filamentous haemagglutinin (fha) concentrations in fermentation broths has been developed. the rapid single-step analysis performed by hydroxyapatite high-performance liquid chromatography using a salt gradient with uv detection allows both the separation of pt from fha and the measurement of their respective concentrations. the assay is highly reproducible. over 35 lots of acellular b. pertussis ... | 1990 | 2172264 |
| mhc-restricted recognition of immunogenic t cell epitopes of pertussis toxin reveals determinants in man distinct from the adp-ribosylase active site. | the s1 subunit of pertussis toxin (pt) is responsible for the reactogenicity and in part the immunogenicity of bordetella pertussis vaccine. the critical residues associated with the immunomodulatory effects of pt were located around glu140 in the s1 subunit. in man, t cell responses to pt are directed at s1 peptides distinct from glu140. two such epitopes, p64-75 and p151-161, are immunogenic in a panel of individuals covering a wide range of hla genotypes. the response to pt peptides is hla cl ... | 1988 | 2460578 |
| methods for isolation of bordetella pertussis from patients with whooping cough. | culture remains the standard method for diagnosis of whooping cough. while in the past attempts at isolating bordetella pertussis from patients with suspected whooping cough were often unsuccessful, new methods have recently been developed which are suitable for use in routine microbiology laboratories. recent advances include the use of calcium alginate tipped swabs for taking nasopharyngeal swabs, use of charcoal horse blood agar for transport and culture, and the inclusion of cephalexin as a ... | 1988 | 2461860 |
| expression of pertussis toxin correlates with pathogenesis in bordetella species. | pertussis toxin is a principal determinant of virulence produced by bordetella pertussis in the disease whooping cough and is the primary toxinogenic component of the pertussis vaccine. this toxin is not produced by the closely related species bordetella parapertussis. toxinogenic strains of b. parapertussis were constructed by the conjugative introduction of cloned pertussis toxin genes. these and analogous toxinogenic and nontoxinogenic strains of b. pertussis were assayed for their toxicity a ... | 1989 | 2464653 |
| g proteins couple alpha-adrenergic and gabab receptors to inhibition of peptide secretion from peripheral sensory neurons. | regulation of neuronal calcium channels by gtp-binding proteins (g proteins) is likely to be an important mechanism by which inhibitory transmitters influence excitation-secretion coupling in presynaptic nerve endings. here, we report that in peripheral sensory neurons from embryonic chick dorsal root ganglia (drg), the g protein-mediated inhibition of voltage-dependent calcium channels may best explain how norepinephrine (ne) and gaba inhibit the electrically evoked, calcium-dependent release o ... | 1989 | 2465394 |
| identification of a common domain in calmodulin-activated eukaryotic and bacterial adenylate cyclases. | bordetella pertussis and bacillus anthracis, two taxonomically distinct bacteria, secrete adenylate cyclase toxins that are activated by the eukaryotic protein calmodulin. the two enzymes contain a well-conserved stretch of 24 amino acid residues [escuyer et al. (1988) gene 71, 293-298]. antibodies have been obtained against two synthetic heptadecapeptides, covering part of the conserved sequences. the anti-peptide antibodies specifically reacted in western blots with the rat brain adenylate cyc ... | 1989 | 2470405 |
| comparison of corynebacterium parvum and bordetella pertussis with freund's complete adjuvant as immunopotentiators for beta-human chorionic gonadotropin linked to an atoxic fragment of tetanus toxin. | corynebacterium parvum and bordetella pertussis were compared with freund's complete adjuvant (fca) for their abilities to potentiate the immune response to haptenic beta-human chorionic gonadotropin covalently coupled to an atoxic 54,000-molecular-weight fragment of tetanus toxin (beta-hcg-ttii). the ability of each adjuvant to enhance production of antibodies to hcg in rabbits was measured by 125i-hcg radioimmunoassay. at sera dilutions of 1:10,000, analysis of variance for the 8-week postimmu ... | 1989 | 2475138 |
| evidence that the adenylate cyclase secreted from bordetella pertussis does not enter animal cells by receptor-mediated endocytosis. | bordetella pertussis, the pathogen responsible for whooping cough, produces a calmodulin-sensitive adenylate cyclase. several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular camp levels (confer and eaton: science 217:948-950, 1982; shattuck and storm: biochemistry 24:6323-6328, 1985). however, the mechanism for entry of the catalytic subunit of this adenylate cyclase into animal cells is unknown. it has been r ... | 1990 | 2177058 |
| encephalitogenic t cells in the b10.pl model of experimental allergic encephalomyelitis (eae) are of the th-1 lymphokine subtype. | t helper cells reactive to myelin basic protein are clearly implicated in the pathogenesis of murine eae. we have developed a t cell line, bml-1 that (1) is reactive to the encephalitogenic amino terminal nonapeptide (1-9nac) of mbp, (2) is i-au restricted, and (3) induces relapsing eae in b10.pl (h-2u) mice. measurement of the lymphokine profile of bml-1 revealed secretion of il-2, interferon-gamma and lymphotoxin but not il-4. this profile is consistent with the th1/dth subtype. coculture of b ... | 1989 | 2478300 |
| location of the three major agglutinogens of bordetella pertussis by immuno-electronmicroscopy. | when the three serotypes of bordetella pertussis (types 1,2,3; 1,2 and 1,3) were labelled with agglutinins and protein-a gold, agglutinogen 1 was found on fimbriae and on the cell surface of types 1,2,3 and 1,2 but on the cell surface only of non-fimbriate type 1,3 organisms. in contrast, agglutinogen 2 was located on fimbriae only. agglutinogen 3 was not labelled. when protein-a gold was replaced by immunoglobulin-g gold, agglutinogen 3 was found on the cell surface only, even of fimbriate bact ... | 1990 | 1971311 |
| multiple t and b cell epitopes in the s1 subunit ("a"-monomer) of the pertussis toxin molecule. | the immunogenicity and reactogenicity of bordetella pertussis vaccine are mediated in part by the s1 subunit of pertussis toxin (pt). to identify the immune epitopes in the s1 subunit of pt, synthetic peptides were prepared and tested for their capacity to induce antibodies in mice with different mhc genotypes. in balb/c mice, peptides corresponding to sequences 1-17, 70-82 and 189-199 generate t cell proliferative responses, induce the production of antibodies capable of neutralization of the t ... | 1989 | 2480389 |
| fimbrial phase variation in bordetella pertussis: a novel mechanism for transcriptional regulation. | fimbriae belong to a class of extracellular filamentous proteins which are involved in the attachment of bacteria to host tissues. bordetella pertussis, the etiological agent of whooping cough, produces two serologically distinct fimbriae. we show that, like a number of other b. pertussis virulence genes, transcription of the fimbrial subunit genes (fim) is positively controlled by trans-acting polypeptides encoded by the bvg locus. in addition to this coordinate control, transcription of the fi ... | 1990 | 1975238 |
| class-specific antibodies to bordetella pertussis, haemophilus influenzae type b, streptococcus pneumoniae and neisseria meningitidis in human breast-milk and maternal-infant sera. | children under 2 years of age are most susceptible to acute respiratory infections caused by bordetella pertussis, haemophilus influenzae type b, streptococcus pneumoniae and neisseria meningitidis. we analysed milk samples and sera from mother-infant pairs for specific antibodies that may enhance protection against the bacterial pathogens. the results show that the breast-milk samples contained significant titres of specific igg and iga antibodies to the four organisms, although the mean igg an ... | 1989 | 2482004 |
| differential coupling with pertussis toxin-sensitive g proteins of dopamine and somatostatin receptors involved in regulation of adenohypophyseal secretion. | d2 dopamine receptors and somatostatin receptors in adenohypophyseal cells are coupled through g proteins to various transduction mechanisms. to study the involvement of these different transduction mechanisms and of various g proteins in the dopamine and somatostatin regulation of prolactin (prl), growth hormone (gh) and thyroid-stimulating hormone (tsh) secretions, we have pretreated the adenohypophyseal cells in primary culture with increasing doses of pertussis toxin. the guanosine triphosph ... | 1990 | 1981365 |
| purification and immunological characterization of a groel-like protein from bordetella pertussis. | a groel-like protein from bordetella pertussis was purified. this protein was found to have the tetradecameric subunit structure typical of the groel family of proteins and to contain epitopes similar to those of other members of this family, including a human groel-like protein. active immunization of neonatal mice with the b. pertussis groel-like protein provided little protection against an aerosol challenge with b. pertussis. antibodies to this protein were elicited in mice by a standard dip ... | 1991 | 2004820 |
| role of interferon-gamma in the modulation of the ige response by 2,4-dinitrophenyl-bordetella pertussis vaccine in the mouse. | immunization of mice with 2,4-dinitrophenyl-bordetella pertussis (dnp-bp) failed to induce anti-dnp ige responses. administration of dnp-bp induced, however, the formation of anti-dnp ige b memory cells, as demonstrated by adoptive transfer. furthermore, mice pretreated with dnp-bp and primed with 2 micrograms dnp-ovalbumin (oa) in alum 2 weeks later produced high day-7 anti-dnp ige levels. these subsided to near undetectable levels by day 12-14. the transient expression of serum ige levels was ... | 1989 | 2495968 |
| experimental autoallergic sialadenitis in the lew rat. i. parameters of disease induction. | experimental autoallergic sialadenitis (eas) is an autoimmune mononuclear cell infiltration of the submandibular salivary gland that results in tissue destruction and glandular dysfunction. a previous report has described an animal model of induced eas in lew rats following sensitization with allogeneic wf submandibular gland (smg). the present study extends this observation to an eas disease model induced following sensitization of lew rats with syngeneic lew smg. furthermore, we describe the c ... | 1991 | 2036674 |
| adenylate cyclase toxins from bacillus anthracis and bordetella pertussis. different processes for interaction with and entry into target cells. | adenylate cyclase (ac) toxins produced by bacillus anthracis and bordetella pertussis were compared for their ability to interact with and intoxicate chinese hamster ovary cells. at 30 degrees c, anthrax ac toxin exhibited a lag of 10 min for measurable camp accumulation that was not seen with pertussis ac toxin. this finding is consistent with previous data showing inhibition of anthrax ac toxin but not pertussis ac toxin entry by inhibitors of receptor-mediated endocytosis (gordon, v. m., lepp ... | 1989 | 2504710 |
| the adipocyte go alpha-immunoreactive polypeptide is different from the alpha subunit of the brain go protein. | rat adipose tissue possesses two bordetella pertussis toxin (ptx) substrates and, in the same 39-41 kda molecular mass range, positive immunoreactivity has also been reported with antibodies against the alpha subunit of go, the major brain gtp-binding protein (g-protein). in this study, the presence of the brain go alpha subunit at 39 kda in adipocytes was reassessed, since direct correspondence between ptx substrates and go alpha immunoreactivity has not yet been clearly established. on resolut ... | 1989 | 2505750 |
| role of leukotriene d4 in the early and late pulmonary responses of rats to allergen challenge. | to examine the role of leukotriene d4 (ltd4) in early and late pulmonary responses to antigen, we evaluated the effects of two ltd4, antagonists, mk-571 and fpl-57231, on the changes in pulmonary resistance (rl) in the 8-h period following antigen challenge of allergic rats. a total of 69 rats, aged 6 to 8 wk, were sensitized to subcutaneous ovalbumin (oa, 1 mg) and intraperitoneal bordetella pertussis vaccine (6 x 10(9) bacilli). at 14 days after sensitization, rats were anesthetized with intra ... | 1990 | 2382899 |
| glutaraldehyde in whole-cell bordetella pertussis vaccine. | treatment of the whole-cell bacterial suspensions of bordetella pertussis with 0.05% glutaraldehyde at room temperature for 10 min kills the bacteria and, except for some histamine sensitising activity, almost detoxifies the pertussis toxin. the glutaraldehyde-vaccine is of good potency with a meritorious performance in tests for abnormal toxicity in mice, the leucocytosis-promoting-factor and the mouse weight gain. using glutaraldehyde for the inactivation of whole-cell pertussis vaccine on a c ... | 1989 | 2514517 |
| [a study of the ability of bordetella pertussis toxin to induce the formation of b-suppressors]. | mouse spleen cells not adhering to the plastic surface and b-cells isolated from them were treated with b. pertussis toxin in vitro, washed and injected into recipients (allogeneic, syngeneic, intact or lethally irradiated) whose immune response to sheep red blood cells was then evaluated by jerne's method. treatment with b. pertussis toxin was shown to induce the development of immunosuppressive activity in intact spleen cells and in b-cells, to abolish the activity of memory b-cells and to enh ... | 1989 | 2525309 |
| [laboratory and clinical studies on clarithromycin in pediatrics]. | clarithromycin (te-031, a-56268) is a new 14-membered ring macrolide antibiotic developed by taisho pharmaceutical co., ltd. te-031 has a methoxy group at position 6 in its structure. in the present study, we carried out laboratory and clinical investigations on te-031 in the field of pediatrics. the obtained results are summarized as follows. the antibacterial activity of te-031 was investigated against 16 clinically isolated strains of streptococcus pyogenes, staphylococcus aureus, haemophilus ... | 1989 | 2526242 |
| seroepidemiology of pertussis in the japanese population. | to ascertain just how widely bordetella pertussis infection occurs in japan, we assessed the immune level against pertussis in various age-groups in the japanese population. using sera mainly obtained from 6- to 75-year old subjects who visited the tokyo metropolitan komagome hospital, we determined the levels of bacterial agglutinins to bordetella pertussis, of anti-filamentous hemagglutinin and of anti-pertussis toxin. the bacterial agglutinin titers in the older age-groups seemed to be slight ... | 1989 | 2535885 |
| evolutionary trees for the genus bordetella. | recent data on enzyme electrophoretic mobility and dna sequences for pertussis toxin allow the construction of evolutionary trees for various strains belonging to the genus bordetella. in contrast to previous analyses, these data can be seen to support the separate clustering of bordetella pertussis strains, in agreement with the traditional classification based on other phenotypic characteristics. an earlier argument placing the divergence of b. pertussis and b. parapertussis before 1912 does n ... | 1989 | 2536675 |
| unexpected isolation of bordetella pertussis from patients with acquired immunodeficiency syndrome. | we isolated bordetella pertussis from three patients with acquired immunodeficiency syndrome who underwent diagnostic bronchoscopy for evaluation of respiratory symptoms. the b. pertussis isolates were recovered from medium (charcoal-yeast extract agar) formulated to enhance recovery of legionella spp., and one of the isolates stained positively with antisera directed against legionella spp. | 1989 | 2536756 |
| human serum antibody responses to bordetella pertussis infection and pertussis vaccination. | we used an immunoblotting technique to compare the serum antibody responses to pertussis toxin (pt), filamentous hemagglutinin (fha), a 69-kilodalton (kda) adenylate cyclase-associated protein (69 kd protein), and bordetella pertussis outer membrane proteins (omps) following either b. pertussis infection or immunization with whole-cell pertussis vaccine. infection and vaccination induced nearly equally intense antibody responses to pt and to fha, but vaccination induced stronger antibody respons ... | 1989 | 2536779 |
| dissociation of cell-associated interleukin-1 (il-1) and il-1 release induced by lipopolysaccharide and lipid a. | the capacities of lipopolysaccharide (lps) and lipid a to trigger mouse balb/c peritoneal macrophages and to induce the production of cell-associated interleukin-1 (il-1) and membrane-associated il-1 and il-1 release have been compared. bordetella pertussis lipid a was 1,000 to 10,000 times less efficient than the native lps to induce il-1 release by freshly isolated elicited macrophages. when resident macrophages were studied, lipid a, at high concentrations (greater than 2 micrograms/ml), indu ... | 1989 | 2537258 |
| deletions affecting hemolytic and toxin activities of bordetella pertussis adenylate cyclase. | the bordetella pertussis cyaa gene encodes a virulence factor which is a bifunctional protein exhibiting calmodulin-sensitive adenylate cyclase and hemolytic activities (p. glaser, h. sakamoto, j. bellahov, a. ullmann, and a. danchin, embo j. 7:3997-4004, 1988). we characterized the hemolytic and toxin activities of the 200-kilodalton (kda) bifunctional (cyaa) protein and showed that, whether cell associated or secreted, the 200-kda cyaa protein carries hemolytic and toxin functions. the catalyt ... | 1990 | 2401563 |
| effects of pertussis toxin treatment on metabolism in hamster brown adipocytes. | this communication reports the effects of the exotoxin of bordetella pertussis (pertussis toxin) on hamster brown fat cells. pertussis toxin significantly increased the lipolytic and respiratory responses to isoproterenol but did not increase the basal rates of either of these processes. in contrast, the stimulation of respiration by the alpha-adrenergic agent phenylephrine was not altered by pertussis toxin. the inhibitory effects of adenosine on stimulated lipolysis, respiration, and adenylate ... | 1985 | 2415001 |
| islet activating protein inhibits physiological responses evoked by cardiac muscarinic acetylcholine receptors. role of guanosine triphosphate binding proteins in regulation of potassium permeability. | the involvement of gtp binding proteins in muscarinic acetylcholine receptor (machr) mediated responses of cultured chick embryonic cardiac muscle cells was studied by using islet activating protein (iap) from bordetella pertussis. incubation of cells for 24 h with iap resulted in inhibition of subsequent iap-catalyzed incorporation of [alpha-32p]adp-ribose into membrane proteins of mr 39 000 (no alpha) and 41 000 (ni alpha); treatment of cultures with 5 ng/ml iap was sufficient to adp-ribosylat ... | 1985 | 2418867 |
| molecular requirement for interleukin 1 induction by lipopolysaccharide-stimulated human monocytes: involvement of the heptosyl-2-keto-3-deoxyoctulosonate region. | experiments were undertaken to localize in the lipopolysaccharide (lps) the minimal structural determinants sufficient to initiate the signal leading to interleukin 1 (il 1) secretion by human monocytes. our results clearly demonstrated that this signal is triggered by structures present in the so-called inner-core region which chemically consists of 2-keto-3-deoxy-d-manno-octulosonic acid (kdo) and heptose in many lps of gram-negative bacteria. thus, the isolated polysaccharide region of bordet ... | 1986 | 2419139 |
| modulation of invasiveness and catalytic activity of bordetella pertussis adenylate cyclase by polycations. | penetration of bordetella pertussis adenylate cyclase into cho cells was monotonically inhibited by polylysines, with a minimum degree of polymerization of greater than 6 and less than or equal to 9 to 10. above this level, inhibitory potency per lysyl residue was independent of polymer length; 50% inhibition was obtained with 60 microm lysine monomer. other polycations were also potent inhibitors. the adenylate cyclase itself showed a biphasic (stimulation-inhibition) response, with a similar i ... | 1989 | 2538395 |
| opioid receptors in magnesium-digitonin-solubilized rat brain membranes are tightly coupled to a pertussis toxin-sensitive guanine nucleotide-binding protein. | opioid receptors solubilized in mg2+-digitonin (2%, wt/vol) from mg2+-pretreated rat brain membranes maintain, in addition to high-affinity opioid agonist binding, the modulation by guanine nucleotides. one of the modes of expression of the latter property is an attenuation of agonist binding by guanine nucleotides in the presence of na+. to investigate the molecular basis of this modulation and to identify the g protein(s) involved, the soluble receptors were [32p]adp-ribosylated by means of bo ... | 1989 | 2538569 |
| filamentous hemagglutinin of bordetella pertussis: nucleotide sequence and crucial role in adherence. | filamentous hemagglutinin is a surface-associated adherence protein of bordetella pertussis, which is a component of some new acellular pertussis vaccines. the nucleotide sequence of an open reading frame that encompasses the filamentous hemagglutinin structural gene, fhab, suggests that proteolytic processing is necessary to generate the mature 220-kda filamentous hemagglutinin product. an arg-gly-asp (rgd) tripeptide is found within filamentous hemagglutinin that may be involved in its adheren ... | 1989 | 2539596 |
| characterization of the bacterial cell associated calmodulin-sensitive adenylate cyclase from bordetella pertussis. | bordetella pertussis produces a calmodulin-sensitive adenylate cyclase that is associated with the whole bacteria and released into its culture media. preparations of this enzyme invade animal cells, causing elevations in intracellular camp levels. cell-associated adenylate cyclase accounted for 28% of the total adenylate cyclase activity while 72% was released into the culture supernatant. over 90% of the cell-associated adenylate cyclase activity was sensitive to trypsin treatment of whole cel ... | 1989 | 2540797 |
| gas chromatographic determination of (phosphorylated) 2-keto-3-deoxyoctonic acid, heptoses and glucosamine in bacterial lipopolysaccharides after treatment with hydrofluoric acid, methanolysis and trifluoroacetylation. | quantification of phosphorylated sugar constituents of lipopolysaccharides has been performed by the following sequence: dephosphorylation by treatment with hydrofluoric acid, cleavage to monomeric constituents by methanolysis and analysis of the released sugars by capillary gas chromatography. lipopolysaccharides of salmonella minnesota rd1p+, bordetella pertussis nih 114 and vibrio cholerae, nag and 95r strains, were used as model substances. comparison of the chromatographic data obtained fro ... | 1989 | 2541150 |
| recovery of bordetella pertussis from nasopharyngeal swabs. | | 1989 | 2541172 |
| primary structure of the peptidoglycan-derived tracheal cytotoxin of bordetella pertussis. | the etiological agent of whooping cough, bordetella pertussis, destroys the ciliated epithelial cells lining the large airways of infected individuals. this cytopathology can be reproduced in respiratory epithelium by tracheal cytotoxin (tct), a small peptidoglycan-related molecule purified from the culture supernatant of growing b. pertussis organisms. using fast atom bombardment mass spectrometry, we analyzed the positive- and negative-ion spectra of the purified, biologically active material ... | 1989 | 2541765 |
| identification of residues essential for catalysis and binding of calmodulin in bordetella pertussis adenylate cyclase by site-directed mutagenesis. | in order to identify molecular features of the calmodulin (cam) activated adenylate cyclase of bordetella pertussis, a truncated cya gene was fused after the 459th codon in frame with the alpha-lacz' gene fragment and expressed in escherichia coli. the recombinant, 604 residue long protein was purified to homogeneity by ion-exchange and affinity chromatography. the kinetic parameters of the recombinant protein are very similar to that of adenylate cyclase purified from b.pertussis culture supern ... | 1989 | 2542030 |
| in vivo beta 2-adrenoceptor hyporesponsiveness in the cardiovascular system of the pig after bordetella pertussis. | the effects of bordetella pertussis (b. pertussis) on autonomic receptor function were investigated in vivo in the cardiovascular and in particular in the coronary vascular system of the pig. for this purpose the pigs were injected i.p. with b. pertussis vaccine 4 days prior to the experiment. blood pressure, heart rate, cardiac output, coronary flow, left ventricular pressure and its dp/dt were measured. b. pertussis induced an increase in the baseline values for heart rate, cardiac output, cor ... | 1989 | 2542052 |
| dissociation of catalytic and invasive activities of bordetella pertussis adenylate cyclase. | bordetella pertussis organisms secrete adenylate cyclase, at least one form of which can invade host cells and appears to be a virulence factor. treatment of urea extracts containing invasive cyclase of b. pertussis with trypsin, chymotrypsin, or subtilisin abolishes the ability to increase intracellular cyclic amp levels in cho cells (invasiveness) at concentrations that have minimal or no effects on adenylate cyclase activity. higher protease concentrations can inhibit catalytic activity, and ... | 1989 | 2542162 |
| rapid detection of bordetella pertussis by a monoclonal antibody-based colony blot assay. | monoclonal antibodies to bordetella pertussis filamentous hemagglutinin (fha) and lipopolysaccharide (lps) were used in a colony blot enzyme-linked immunosorbent assay designed for rapid detection of b. pertussis. bacterial colonies from bordet-gengou agar plates were blotted onto nitrocellulose filter disks, lysed by immersion in chloroform, and reacted with monoclonal antibodies. following reaction with peroxidase-conjugated rabbit anti-mouse immunoglobulin antisera and 4-chloro-1-naphthol, bl ... | 1989 | 2542357 |
| evaluation of culture, immunofluorescence, and serology for the diagnosis of pertussis. | nasopharyngeal culture, direct immunofluorescence, and serology of acute-phase and paired serum specimens were compared for the laboratory diagnosis of infections due to bordetella pertussis in a community-based pediatric population with both high vaccine usage and high pertussis incidence. in 77 (37%) of 210 patients evaluated, one or more tests were positive for pertussis. a clinical illness compatible with pertussis was present in 52 (71%) of 73 pertussis test-positive and 42 (35%) of 119 tes ... | 1989 | 2542366 |
| molecular cloning and characterization of protective outer membrane protein p.69 from bordetella pertussis. | protein p.69 is localized on the outer membrane of bordetella pertussis and is one of the virulence factors believed to contribute to the disease state of whooping cough. we demonstrate that protein synthesis of p.69 is under genetic control of the vir locus. using oligonucleotide probes derived from the protein sequence of a cyanogen bromide fragment, we have cloned the gene for p.69 from b. pertussis cn2992. analysis of the dna sequence reveals a g + c-rich gene capable of encoding a protein o ... | 1989 | 2542937 |
| inhibition of immunological and nonimmunological histamine release from human basophils by adenosine analogues that act at p-sites. | 2',5'-dideoxyadenoside (dda) inhibited both anti-ige and ionophore a23187 induced histamine secretion from human basophils. whereas dda inhibited ige-dependent histamine secretion when added at all times prior to challenge, release induced by a23187 was inhibited only with simultaneous addition of dda and secretagogue. dipyridamole, but not theophylline, abrogated dda mediated inhibition of histamine release suggesting an intracellular mechanism of action of dda. the observations that 2'-deoxyad ... | 1986 | 2424453 |
| biological activities and chemical composition of purified tracheal cytotoxin of bordetella pertussis. | specific destruction of ciliated epithelial cells lining the large airways is the primary respiratory tract cytopathology associated with human bordetella pertussis infections. we have purified a single low-molecular-weight glycopeptide, tracheal cytotoxin (tct), that appears to cause this pathology. by using a combination of solid-phase extraction and reversed-phase high-pressure liquid chromatography, about 700 nmol of biologically active peptide can be isolated from 1 liter of b. pertussis cu ... | 1989 | 2543636 |
| the gtp-binding protein, go, regulates neuronal calcium channels. | in neuronal cells, opioid peptides and opiates inhibit neurotransmitter release, which is a calcium-dependent process. they also inhibit adenylyl cyclase, presumably via the membrane signal-transducing component, gi, a guanine nucleotide-binding protein (g-protein). no causal relationship between these two events has yet been demonstrated. besides gi, membranes of neuronal tissues contain large amounts of go, a g-protein with unknown function. both g-proteins are heterotrimers consisting of alph ... | 1987 | 2433590 |
| unusual composition of peptidoglycan in bordetella pertussis. | the composition of the peptidoglycan of bordetella pertussis and the nature of its turnover products was determined by a new combination of analytical techniques: high performance liquid chromatography of an enzymatic peptidoglycan hydrolysate and fast atom bombardment mass spectrometry and fast atom bombardment collision-activated dissociation tandem mass spectrometry. sixteen major components of the peptidoglycan were purified, and assignment of complete or partial chemical structures was achi ... | 1989 | 2544584 |
| intratumoral induction of tumour necrosis factor by systemic administration of bordetella pertussis vaccine. | intratumoral induction of tumour necrosis factor (tnf) by administration of bordetella pertussis vaccine (bpv) as compared with that by the agent ok-432 was investigated in mice. two hours after such administration tumour tissues tested were resected from the mice, homogenised, and the tnf activities in the homogenate were assayed using a l-929 fibroblast assay. intravenous injection of bpv into mice bearing the mm46 carcinoma resulted in a greater concentration of tnf in the tumour homogenate t ... | 1990 | 2206945 |
| [immunobiologic activity of bordetella pertussis strains defective in various virulence factors]. | the immunobiological properties of mutant strains, selectively deprived of certain antigens (hemagglutinin, b. pertussis toxin, dermonecrotic toxin, hemolysin, adenylate cyclase), have been studied with the aim of finding out the relationship between the presence of certain antigens in microbial strains and their protective properties. the results of these studies suggest that the protective potency of pertussis vaccine may be related to the presence of some antigenic substances, including those ... | 1989 | 2545060 |
| site-directed mutagenesis of lysine 58 in a putative atp-binding domain of the calmodulin-sensitive adenylate cyclase from bordetella pertussis abolishes catalytic activity. | a 2.7-kb cya a gene fragment encoding the amino-terminal end of the calmodulin-sensitive adenylate cyclase from bordetella pertussis has been placed under the control of the lac promoter for expression in escherichia coli. following induction with isopropyl beta-d-thiogalactoside, calmodulin-sensitive adenylate cyclase activity was detected in a cell extract from e. coli. the expression vector directed the synthesis of a 90-kda polypeptide that was recognized by rabbit polyclonal antibodies rais ... | 1989 | 2545236 |
| comparison of four charcoal media for the isolation of bordetella pertussis. | charcoal-horse blood agar with 40 micrograms of cephalexin per ml, charcoal-horse blood agar with 3 micrograms of lincomycin per ml, charcoal agar with 3 micrograms of lincomycin per ml, and legionella (buffered charcoal-yeast extract) agar with 3 micrograms of lincomycin per ml were compared for isolation of bordetella pertussis. charcoal-horse blood agar with 40 micrograms of cephalexin per ml gave the best results, with a b. pertussis recovery rate of 100%. growth was most rapid and the mean ... | 1989 | 2545740 |
| expression and secretion of the s-1 subunit and c180 peptide of pertussis toxin in escherichia coli. | the structural gene of the s-1 subunit of pertussis toxin (rs-1) and the catalytic c180 peptide of the s-1 subunit (c180 peptide) were independently subcloned downstream of the tac promoter in escherichia coli. both constructions included dna encoding for the predicted leader sequence of the s-1 subunit which was inserted between the tac promoter and the structural gene. e. coli containing the plasmids encoding for rs-1 and c180 peptide produced a peptide that reacted with anti-pertussis toxin a ... | 1989 | 2546919 |
| [the coleukemogenic action of bordetella pertussis]. | injection of b. pertussis and rauscher leukemia virus (rlv) in a dose of 4 id50 to balb/c mice susceptible to the above virus significantly increases the incidence of leukosis and shortens the average life duration. injection of b. pertussis to the akr mice, carriers of the gross leukosis virus, induces in the first months a greater number of the mice with leukosis and its earlier development. | 1989 | 2547574 |
| invasion of hela 229 cells by virulent bordetella pertussis. | phase-dependent invasive behavior of bordetella pertussis was demonstrated by recovery of viable organisms from gentamicin-treated hela cell monolayers and by transmission electron microscopy. several mutants of b. pertussis with tn5 or tn5 lac inserted into various vir-regulated genes were evaluated for differences in their invasive abilities. mutants lacking filamentous hemagglutinin, pertussis toxin, and two as yet uncharacterized vir-regulated products had levels of invasion significantly lo ... | 1989 | 2547718 |
| effects of phosphatidic acid on islet cell phosphoinositide hydrolysis, ca2+, and adenylate cyclase. | phosphatidic acid may be raised in glucose-stimulated islet cells through hydrolysis of phosphatidylinositol 4,5-bisphosphate (pip2) and de novo synthesis with glucose-derived trioses. the mechanism by which exogenous phosphatidic acid from egg yolk lecithin may augment insulin secretion was investigated in neonatal beta-cells. in whole cells labeled with [2,8-3h]-adenine, a dose-dependent increase in phosphatidic acid-stimulated adenylate cyclase activity was seen, and a small intracellular tra ... | 1989 | 2548909 |
| comparison of erythromycin ethylsuccinate and co-trimoxazole for treatment of pertussis. | fifty-five ambulatory children with early culture-proven pertussis were treated for two weeks either with erythromycin ethylsuccinate (n = 28) (50-80 mg/kg/day in three doses during meals) or with co-trimoxazole (n = 27) (6-10 mg trimethoprim/kg/day in two doses after meals). after completion of treatment, all patients in the erythromycin group were culture-negative, while in the co-trimoxazole group one child was still culture-positive. in this case vomiting may have played a role. both agents ... | 1989 | 2548964 |
| the location of filamentous hemagglutinin and pertussis toxin antigens of bordetella pertussis by immunoelectron microscopy. | immunoelectron microscopy using colloidal gold-tagged antibodies was used to detect filamentous hemagglutinin (fha) and pertussis toxin (pt) antigens on the surface and in the cytoplasm of bordetella pertussis cells. both gold-tagged antibodies to fha and pt labeled the aggregates of filamentous material on the surface of sediment-settled phase i cells under static conditions. fha and pt antigens were detected also on ultrathin sections made after embedding the phase i cells in lowicryl k4m resi ... | 1989 | 2549145 |
| role of pertussigen (pertussis toxin) on the mouse protective activity of vaccines made from bordetella species. | pertussigen [pertussis toxin (ptx)] from bordetella pertussis, when detoxified, induces protection in mice to intracerebral challenge (ic) with virulent b. pertussis. in its native form, minute nonprotective doses promote the development of immunity induced by other antigens of b. pertussis. as little as 4 ng of ptx, given with a nonprotective dose of 8 x 10(7) killed cells of the phase iii sakairi strain, promoted detectable protection to ic challenge. native ptx in doses of 0.4 to 400 ng did n ... | 1989 | 2549344 |
| interleukin-1 induction by lipopolysaccharides: structural requirements of the 3-deoxy-d-manno-2-octulosonic acid (kdo). | we previously showed the importance of the 3-deoxy-d-manno-2-octulosonic acid (kdo) residue in endotoxins (lipopolysaccharides, lps) for the induction of the synthesis and release of interleukin-1 (il-1) by human monocytes. we further investigated the effect of some structural variations within the kdo molecule on il-1 production induced by lps. deamination of bordetella pertussis lps, followed by mild anhydrous acidic methanolysis released a hexasaccharide (fragment b'), which had a terminal me ... | 1989 | 2549403 |
| sequences required for expression of bordetella pertussis virulence factors share homology with prokaryotic signal transduction proteins. | the bvg locus of bordetella pertussis is required for coordinate regulation of several factors associated with virulence. the control system is modulated by various environmental signals, including low temperature, mgso4, and nicotinic acid. the nucleotide sequence of the bvg region has been determined and three open reading frames, bvga, bvgb, and bvgc, are present. twelve-base-pair linker insertion mutations in any of these open reading frames result in a bvg- phenotype. the predicted protein ... | 1989 | 2549542 |
| detection of bordetella pertussis by determination of adenylate cyclase activity. | the adenylate cyclase activity of bordetella pertussis in clinical isolates was measured in calmodulin-supplemented stainer-scholte broth by the rate of conversion of atp to cyclic amp. analysis of 250 stock strains of bordetella pertussis showed that measurable adenylate cyclase activity was produced by all strains. in clinical tests bordetella pertussis was isolated from 135 (22%) of 605 swab samples. increased adenylate cyclase activity was detected in 124 (92%) stainer-scholte broth cultures ... | 1989 | 2550232 |
| activity of new macrolides against bordetella pertussis and bordetella parapertussis. | mics and mbcs of four new macrolides (azithromycin, clarithromycin, dirithromycin and roxithromycin) and two older macrolides (erythromycin and josamycin) for bordetella pertussis and bordetella parapertussis were determined. the activity of the new macrolides was as good as that of erythromycin, while josamycin was slightly less active. bordetella parapertussis was more resistant than bordetella pertussis. | 1989 | 2550234 |
| comparison of three kinds of blood and two incubation atmospheres for cultivation of bordetella pertussis on charcoal agar. | we compared the growth of bordetella pertussis strains (n = 32) on antibiotic-free and cephalexin (40 micrograms/ml)-containing charcoal agar supplemented with 10% defibrinated horse blood, defibrinated sheep blood, or anticoagulant-containing human blood. plates were incubated either in air or in an atmosphere with 5 to 10% co2. as assessed by mean colony numbers and rapidity of growth, normal air was preferable to co2 enrichment for incubation. growth on horse blood agar was more abundant and ... | 1989 | 2550521 |
| the subunit s1 is important for pertussis toxin secretion. | pertussis toxin is a protein containing five noncovalently linked subunits which are assembled into the monomer a (containing the subunit s1) and the oligomer b (containing subunits s2, s3, s4, and s5 in a 1:1:2:1 ratio). each of the five subunits is synthesized as a precursor containing a secretory leader peptide and is secreted into the periplasm of bordetella pertussis where the five subunits are assembled into the oligomeric structure and then released into the culture medium. in the absence ... | 1990 | 2211659 |
| [efficacy of acellular pertussis vaccine]. | an outbreak of pertussis occurred in one room of a residential facility where 19 children aged 5 to 36 months were residing. they were prospectively surveyed to estimate the efficacy of acellular pertussis vaccine. among the 19 residents, 9 were immunized with acellular pertussis vaccine. among the 19 residents, 9 were immunized with acellular dtp vaccine and 10 were unimmunized. the spread of pertussis was surveyed bacteriologically and serologically for 2 months. among the 9 immunized, 7 child ... | 1990 | 2212750 |
| cloning and expression of mouse-brain calmodulin as an activator of bordetella pertussis adenylate cyclase in escherichia coli. | cloning of higher eukaryotic genes has seldom been performed by complementation of a defective prokaryotic function. this is especially true in the case of functions that are normally absent from the prokaryotic host. we demonstrate here that it is possible to identify by complementation the cdna from mouse brain, which encodes calmodulin (cam) synthesis, in spite of the fact that the recipient strain, escherichia coli, does not normally harbour a cam function. a three-component cloning procedur ... | 1989 | 2551780 |
| analysis of the chromosomal location of two copies of a bordetella pertussis insertion sequence. | is481v1 and is481v2 are two copies of a bordetella pertussis insertion sequence element. we have shown that is481v1 is located within 3 kbp of the start of the adenylate cyclase gene whilst is481v2 is immediately adjacent to the end of the agglutinogen 2 gene and provides the stop codon for that gene. in addition, is481v1 and is481v2 were present at these two specific sites in nine strains of b. pertussis, including two phase iv strains which expressed neither adenylate cyclase nor agglutinogen ... | 1989 | 2552259 |
| [isolation of hemolytic cultures of bordetella pertussis intended for the production of acellular pertussis preparations and the characterization of their biological properties]. | the heterogeneity of the population of b. pertussis laboratory strains with respect to the capacity of individual clones to lyse erythrocytes has been established. the complete or partial reduction of the antigens under study in nonhemolytic bacteria has been shown. the use of hemolytic cultures for the preparation of acellular pertussis vaccine makes it possible to increase the content of b. pertussis toxin in these vaccines. | 1989 | 2552711 |
| gtp gamma s increases thrombin-mediated inositol trisphosphate accumulation in permeabilized human endothelial cells. | ca2+-mobilizing agonists stimulate phospholipase c-mediated phosphatidylinositol 4,5-bisphosphate hydrolysis and inositol trisphosphate (ip3) formation in pulmonary as well as in peripheral vascular endothelial cells (ec). in general, it is believed that receptor-phospholipase c interactions involve a guanine nucleotide regulatory (g) protein. this interaction can be inhibited by bordetella pertussis toxin in certain cells. here we report that pertussis toxin catalyzes the [32p]adp ribosylation ... | 1989 | 2552882 |
| the bvga gene of bordetella pertussis encodes a transcriptional activator required for coordinate regulation of several virulence genes. | the bvg region of the respiratory pathogen bordetella pertussis coordinately regulates the expression of several unlinked virulence determinants in response to environmental signals. the dna sequence of the bvg region contains three genes (bvga, bvgb, and bvgc). transcription of a single-copy fusion consisting of the upstream region of a bvg-activated b. pertussis gene (fhab) attached to the promoterless lac operon in escherichia coli requires the entire bvgabc region in trans. activation of the ... | 1989 | 2553677 |
| analysis of bordetella pertussis virulence gene regulation by use of transcriptional fusions in escherichia coli. | the virulence regulon of bordetella pertussis includes a trans-acting regulatory locus, bvg, that is required for expression of several virulence factors. the virulence control system also responds to environmental signals. we have reconstructed a bvg-dependent regulatory system in escherichia coli by using bacteriophage lambda vectors carrying transcriptional fusions to laczya. single-copy laczya fusions to the b. pertussis fhab locus, which encodes the attachment factor filamentous hemagglutin ... | 1989 | 2553678 |
| adenylate cyclase toxin from bordetella pertussis. identification and purification of the holotoxin molecule. | bordetella pertussis adenylate cyclase (ac) toxin is a calmodulin-activated adenylate cyclase enzyme which has the capacity to enter eukaryotic target cells and catalyze the conversion of endogenous atp into cyclic amp. in this work, the ac holotoxin molecule is identified and isolated. it is a single polypeptide of apparent 216 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. monoclonal antibodies which immunoprecipitate ac activity from extracts of wild type b. p ... | 1989 | 2553737 |
| pertussis (whooping cough) toxin and bordetella pertussis whole-cell antibody levels in a healthy new zealand population. | enzyme linked immunosorbent assay (elisa) tests were used to measure igg antibody levels to bordetella pertussis whole-cell and to pertussis toxin in a healthy new zealand population. the percentage of individuals with measurable antibody to b pertussis whole-cell increased from 47.3% in the 5 year olds to 93.0% in the 40-49 age group, from which the percentage with antibody dropped to 88.7% in the 50-65 age group. the percentage of persons with antibody to the toxin increased with age from 17.5 ... | 1989 | 2554227 |
| positive transcriptional feedback at the bvg locus controls expression of virulence factors in bordetella pertussis. | | 1990 | 2263607 |
| pertussis holotoxoid formed in vitro with a genetically deactivated s1 subunit. | the cytotoxicity of pertussis toxin, a multisubunit exotoxin produced by bordetella pertussis, is believed to be due to the adp-ribosyltransferase activity of the s1 subunit. we have previously described the recombinant expression of each of the five individual pertussis toxin subunits in escherichia coli and the production of an enzymatically deficient form of the s1 subunit by site-directed mutagenesis. we now report the in vitro assembly of holotoxin from native pertussis toxin b oligomer and ... | 1989 | 2554311 |
| [genetic control of experimental autoimmune uveitis in rats]. | differences in susceptibility to experimental autoimmune uveitis (eau) among rats of various strains have been reported. the present study was aimed at separating the effects of the major histocompatibility antigen complex (mhc) and non-mhc genes on the development of eau. eau-susceptible lew, eau-resistant wkah, wkah, 1l mhc congenic strain rats representing mhc of lew on wkah genetic background, and other nine inbred strains of rats were examined for their ability to develop eau by immunizatio ... | 1990 | 2265820 |
| bordetella pertussis adenylate cyclase: purification and characterization of the toxic form of the enzyme. | bordetella pertussis produces a calmodulin-sensitive adenylate cyclase (ac) which is an essential virulence factor in mammalian pertussis. here we report the purification and characterization of the toxic form of the enzyme, which penetrates eukaryotic cells and generates high levels of intracellular camp. this form was purified from an extract of b.pertussis strain carrying a recombinant plasmid which over-produced both enzymatic and toxic activities of the enzyme. western blot analysis of the ... | 1989 | 2555185 |
| preparation of filamentous hemagglutinin from bordetella pertussis and assay for serum antibodies to filamentous hemagglutinin and pertussis toxin for clinical and public health laboratories. | a procedure that is sufficiently simple and economical for use in clinical and public health laboratories for producing and purifying filamentous hemagglutinin (fha) and determining antibodies to this major antigen of bordetella pertussis in serum is described. high yields of fha (40 to 80 mg/liter) were obtained in the supernatant by cultivating b. pertussis in modified cl medium. the fha antigen was separated from pertussis toxin (pt) and other antigens by chromatography on hydroxylapatite. re ... | 1989 | 2556434 |
| transfer of susceptibility to experimental autoimmune orchitis from responder to non-responder substrains of balb/c mice. | substrains of balb/c mice differ in their susceptibility to experimental autoimmune orchitis (eao), with balb/cj representative of the non-responders and balb/cby representative of the responders. we examined whether the susceptibility of these two substrains could be altered by reciprocal adoptive transfer of lymphoid cells. the cells transferred were of three types, normal spleen cells, t cell-enriched spleen and lymph node cells from mice immunized with testis homogenate (th) in complete freu ... | 1990 | 2266540 |
| variations in the carbohydrate regions of bordetella pertussis lipopolysaccharides: electrophoretic, serological, and structural features. | structural and immunological differences between the two components that are usually present in unequal quantities in bordetella pertussis endotoxin preparations and are visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis have been studied by using strains 1414, a100, and 134, all in phase i. according to analyses by both sodium dodecyl sulfate-polyacrylamide gel electrophoresis and thin-layer chromatography, the minor (8%) component of the endotoxin of strain 1414 (endotoxin ... | 1990 | 2298694 |
| antibodies to bordetella pertussis adenylate cyclase are produced in man during pertussis infection and after vaccination. | bordetella pertussis produces several potential virulence factors. one of these is an adenylate cyclase which penetrates eukaryotic cells, is activated by calmodulin and generates high levels of intracellular camp. we have found that pertussis infection in man leads to production of high titres (2000-8000) of anti-b. pertussis adenylate cyclase antibodies. such antibodies also are produced after pertussis vaccination. they persist into adulthood, cross the placenta and disappear a few months aft ... | 1990 | 2374155 |
| antigenic determinants of lipid a analyzed with synthetic models and monoclonal antibodies. | three monoclonal antibodies directed against the hydrophobic region (lipid a backbone) of bordetella pertussis endotoxin were used to analyze cross reactivities of lipid a and synthetic glycolipids structurally related to it. reactivity of mab with synthetic compounds was measured by a radioimmunoassay inhibition test. our data indicate that 3-acyloxyacyl groups do not play a significant role, and suggest that a phosphate and a hydroxyl group, at two carbon distance from each other, are part of ... | 1987 | 2436617 |
| effects of antiallergic drugs on bronchial and cutaneous anaphylaxis in lewis rats. | effects of antiallergic agents on 2,4-dinitrophenylated ascaris extract (dnp-as)-induced bronchial asthma were studied in lewis rats, and compared with those on passive cutaneous anaphylaxis (pca). effects of methysergide and chlorpheniramine on the bronchial asthma model were also investigated. rats were actively sensitized with dnp-as antigen and with killed bordetella pertussis. after 8 d, asthmatic response was provoked by inhalation of dnp-as. the bronchomotor response was measured with a m ... | 1988 | 2454312 |
| dopamine inhibits prolactin secretion stimulated by the calcium channel agonist bay-k-8644 through a pertussis toxin-sensitive g protein in anterior pituitary cells. | in primary culture of anterior pituitary cells, bay-k-8644, a calcium channel agonist, stimulated prl secretion by 83% with ec50 of 18 nm. this effect was blocked by nifedipine, a calcium channel antagonist. the stimulations of prl secretion induced by potassium (50 mm) and bay-k-8644 were additive. dopamine inhibited basal as well as bay-k-8644-stimulated prl secretion by 64% and 75%, respectively, and with respective ec50 values of 4.5 and 0.6 nm. in the presence of 50 mm k+, dopamine only par ... | 1988 | 2454806 |
| single-step purification of pertussis toxin and its subunits by heat-treated fetuin-sepharose affinity chromatography. | a general procedure for purifying biologically active pertussis toxin from bordetella pertussis fermentation broth using affinity chromatography on heat-treated fetuin-sepharose cl-4b is described. diethanolamine is used as eluent in this single-step purification to prepare endotoxin-free pertussis toxin in good yield (70%) and high purity (greater than 95%). this one-step affinity chromatography procedure can be easily applied for large-scale preparation of pertussis toxin s1 subunit and its b- ... | 1989 | 2477043 |
| environmental regulation of expression of virulence determinants in bordetella pertussis. | the trans-activator vir is required for expression of all virulence-associated genes in bordetella pertussis. the nature of the global regulation of these factors by vir and environmental signals was examined by northern blot analysis and with beta-galactosidase transcriptional fusions in five vir-regulated genes. northern blots suggested that vir regulates at the level of transcription since vir- organisms did not exhibit detectable mrna from vir-regulated loci. environmental signals such as hi ... | 1989 | 2478524 |
| effects of lipopolysaccharide on macrophages analyzed with anti-lipid a monoclonal antibodies and polymyxin b. | six monoclonal antibodies (mab) to the lipid a region of bacterial lipopolysaccharide (lps), obtained from mice immunized with lipid a-coated bordetella pertussis cells (mab 3.e8, 2.21, 2.37, 2.41) or with lipid a covalently coupled to keyhole limpet hemocyanin (mab r1 and r7), were examined for their potential to inhibit in vitro activities of lps on macrophages. mab r7 was inactive in vitro, but the five other mab inhibited efficiently some in vitro activities of lps. mab r1, 2.21 and 3.e8 red ... | 1989 | 2481587 |
| engineering bacterial toxin for the development of new vaccine against pertussis. | bordetella pertussis is the causative agent of whooping cough. the cellular pertussis vaccine introduced in the forties is highly effective and is widely used, but its reactogenicity has led to public concern regarding its safety. the attempts to reduce the side effects associated with pertussis immunization have led to the preparation of acellular b. pertussis products: one composed of detoxified pertussis toxin (pt) and filamentous haemagglutinin (fha), another one composed only of detoxified ... | 1988 | 2483870 |