| characterization of 1-aminocyclopropane-1-carboxylate (acc) deaminase containing methylobacterium oryzae and interactions with auxins and acc regulation of ethylene in canola (brassica campestris). | the possible interaction of the plant hormones auxin and ethylene and the role of 1-aminocyclopropane-1-carboxylate (acc) deaminase containing bacteria on ethylene production in canola (brassica campestris) in the presence of inhibitory concentrations of growth regulators were investigated. the effects of auxin (indole-3-acetic acid and 2,4-dichlorophenoxy acetic acid), auxin transport inhibitor 2-(p-chlorophenoxy)-2-methylpropionic acid, ethylene precursor 1-aminocyclopropane-1-carboxylate and ... | 2007 | 17541630 |
| synthesis of c5-dicarboxylic acids from c2-units involving crotonyl-coa carboxylase/reductase: the ethylmalonyl-coa pathway. | fifty years ago, kornberg and krebs established the glyoxylate cycle as the pathway for the synthesis of cell constituents from c2-units. however, since then, many bacteria have been described that do not contain isocitrate lyase, the key enzyme of this pathway. here, a pathway termed the ethylmalonyl-coa pathway operating in such organisms is described. isotopically labeled acetate and bicarbonate were transformed to ethylmalonyl-coa by cell extracts of acetate-grown, isocitrate lyase-negative ... | 2007 | 17548827 |
| expression, purification and preliminary x-ray characterization of dl-2-haloacid dehalogenase from methylobacterium sp. cpa1. | dl-2-haloacid dehalogenase from methylobacterium sp. cpa1 (dl-dex mb) is a unique enzyme that catalyzes the dehalogenation reaction without the formation of an ester intermediate. a recombinant form of dl-dex mb has been expressed in escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. the crystal belongs to the hexagonal space group p6(3), with unit-cell parameters a = b = 186.2, c = 114.4 a. the crystals are likely to contain between four and eight monome ... | 2007 | 17620717 |
| cell-to-cell heterogeneity in growth rate and gene expression in methylobacterium extorquens am1. | cell-to-cell heterogeneity in gene expression and growth parameters was assessed in the facultative methylotroph methylobacterium extorquens am1. a transcriptional fusion between a well-characterized methylotrophy promoter (p(mxaf)) and gfp(uv) (encoding a variant of green fluorescent protein [gfpuv]) was used to assess single-cell gene expression. using a flowthrough culture system and laser scanning microscopy, data on fluorescence and cell size were obtained over time through several growth c ... | 2007 | 17644598 |
| characterization of an iron- and manganese-containing superoxide dismutase from methylobacillus sp. strain sk1 dsm 8269. | a superoxide dismutase was purified 62-fold in seven steps to homogeneity from methylobacillus sp. strain sk1, an obligate methanol-oxidizing bacterium, with a yield of 9.6%. the final specific activity was 4,831 units per milligram protein as determined by an assay based on a 50% decrease in the rate of cytochrome c reduction. the molecular weight of the native enzyme was estimated to be 44,000. sodium dodecyl sulfate gel electrophoresis revealed two identical subunits of molecular weight 23,10 ... | 2007 | 17646712 |
| over-expression of a hydroxypyruvate reductase in methylobacterium sp. mb200 enhances glyoxylate accumulation. | methylobacterium sp. mb200 capable of producing glyoxylate from methanol was obtained by enrichment culture using a medium containing methanol as the sole carbon source. a hpr gene that encodes a hydroxypyruvate reductase (hpr) was cloned from this strain and was ligated into the vector plafr3 to obtain the recombinant plasmid plafrh, which was transferred into m. sp. mb200 to generate an recombinant strain mb201. homologous expression of hpr under the control of the lacz promoter led to the enh ... | 2007 | 17653579 |
| biofilm interactions between distinct bacterial genera isolated from drinking water. | in the environment, multiple microorganisms coexist as communities, competing for resources and often associated as biofilms. in this study, single- and dual-species biofilm formation by, and specific activities of, six heterotrophic intergeneric bacteria were determined using 96-well polystyrene plates over a 72-h period. these bacteria were isolated from drinking water and identified by partial 16s rrna gene sequencing. a series of planktonic studies was also performed, assessing the bacterial ... | 2007 | 17675433 |
| methylobacterium salsuginis sp. nov., isolated from seawater. | three pink-pigmented, facultatively methylotrophic strains, designated mp1, mp2 and mr(t), were isolated from seawater from southern china and characterized. analysis of their complete 16s rrna gene sequences revealed that they constituted three separate phylogenetic groups, showing the highest levels of similarity with respect to some members of the genus methylobacterium. pcr amplification also showed the gene coding for the alpha-subunit of methanol dehydrogenase (mxaf) to be present in all s ... | 2007 | 17684240 |
| metabolite profiling analysis of methylobacterium extorquens am1 by comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry. | methylobacterium extorquens am1 is a facultative methylotroph, which is a potential candidate to be used in commercial processes to convert simple one-carbon compounds to a variety of multicarbon chemicals and products. to better understand c(1) metabolism in m. extorquens am1 at the systems level, metabolite profiling tools were developed and applied in this bacterium. comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (gc x gc-tofms) was used to obta ... | 2008 | 17879968 |
| nodulation of cyclopia spp. (leguminosae, papilionoideae) by burkholderia tuberum. | species of the genus burkholderia, from the betaproteobacteria, have been isolated from legume nodules, but so far they have only been shown to form symbioses with species of mimosa, sub-family mimosoideae. this work investigates whether burkholderia tuberum strains stm678 (isolated from aspalathus carnosa) and dus833 (from aspalathus callosa) can nodulate species of the south african endemic papilionoid genera cyclopia (tribe podalyrieae) and aspalathus (crotalarieae) as well as the promiscuous ... | 2007 | 17881339 |
| identification of bacteria from clinical samples using bartonella alpha-proteobacteria growth medium. | in an effort to overcome historical problems associated with the isolation of bartonella species from animal and human blood samples, our laboratory developed a novel, chemically modified, insect-based, liquid culture medium (bartonella alpha-proteobacteria growth medium, bapgm). in this study, we describe the isolation of non-bartonella bacteria from aseptically obtained human blood and tissue samples that were inoculated into bapgm pre-enrichment culture medium, and were obtained during attemp ... | 2007 | 17889384 |
| thiosulfate oxidation and mixotrophic growth of methylobacterium oryzae. | thiosulfate oxidation and mixotrophic growth with succinate or methanol plus thiosulfate was examined in nutrient-limited mixotrophic condition for methylobacterium oryzae cbmb20, which was recently characterized and reported as a novel species isolated from rice. methylobacterium oryzae was able to utilize thiosulfate in the presence of sulfate. thiosulfate oxidation increased the protein yield by 25% in mixotrophic medium containing 18.5 mmol.l-1 of sodium succinate and 20 mmol.l-1 of sodium t ... | 2007 | 17898842 |
| protracted cervical lymphadenitis with mycobacterium avium and methylobacterium mesophilicum. | | 2007 | 17901817 |
| identification of a fourth formate dehydrogenase in methylobacterium extorquens am1 and confirmation of the essential role of formate oxidation in methylotrophy. | a mutant of methylobacterium extorquens am1 with lesions in genes for three formate dehydrogenase (fdh) enzymes was previously described by us (l. chistoserdova, m. laukel, j.-c. portais, j. a. vorholt, and m. e. lidstrom, j. bacteriol. 186:22-28, 2004). this mutant had lost its ability to grow on formate but still maintained the ability to grow on methanol. in this work, we further investigated the phenotype of this mutant. nuclear magnetic resonance experiments with [13c]formate, as well as 14 ... | 2007 | 17921299 |
| screening and isolation of phb-producing bacteria in a polluted marine microbial mat. | the characteristics of microbial mats within the waste stream from a seafood cannery were compared to a microbial community at a pristine site near a sandy beach at puerto san carlos, baja california sur, mexico. isolation of poly-beta-hydroxybutyrate (phb)-producing bacteria, recognition of brightly refractile cytoplasmatic inclusions, lipophilic stains with sudan black and nile red, and chemical extraction of phb were used as a culture-dependent strategy for the detection of phb-producing bact ... | 2008 | 17965957 |
| phyr is involved in the general stress response of methylobacterium extorquens am1. | phyr represents a novel alphaproteobacterial family of response regulators having a structure consisting of two domains; a predicted amino-terminal extracytoplasmic function (ecf) sigma factor-like domain and a carboxy-terminal receiver domain. phyr was first described in methylobacterium extorquens am1, in which it has been shown to be essential for plant colonization, probably due to its suggested involvement in the regulation of a number of stress proteins. here we investigated the phyr regul ... | 2008 | 18024517 |
| enhanced phb production and scale up studies using cheese whey in fed batch culture of methylobacterium sp. zp24. | methylobacterium sp. zp24 produced polyhydroxybutyrate (phb) from disaccharides like lactose and sucrose. as methylobacterium sp. zp24 showed growth associated phb production, an intermittent feeding strategy having lactose and ammonium sulfate at varying concentration was used towards reaching higher yield of the polymer. about 1.5-fold increase in phb production was obtained by this intermittent feeding strategy. further increase in phb production by 0.8-fold could be achieved by limiting the ... | 2008 | 18032031 |
| methylobacterium platani sp. nov., isolated from a leaf of the tree platanus orientalis. | a novel bacterial strain, designated pmb02(t), was isolated from a leaf of the tree platanus orientalis. colonies grown on tyg agar plates were circular, pink-pigmented and slow-growing, being 0.2-1.5 mm in diameter after 3 days growth. the cells of strain pmb02(t) were gram-negative, aerobic, motile rods that possessed oxidase and catalase activities and grew at 20-30 degrees c, ph 6-8 and in media containing less than 1 % nacl. the major respiratory quinone was identified as q-10. a phylogenet ... | 2007 | 18048737 |
| production of acyl-homoserine lactone quorum-sensing signals is widespread in gram-negative methylobacterium. | members of methylobacterium, referred as pink-pigmented facultative methylotrophic bacteria, are frequently associated with terrestrial and aquatic plants, tending to form aggregates on the phyllosphere. we report here that the production of autoinducer molecules involved in the cell-to-cell signaling process, which is known as quorum sensing, is common among methylobacterium species. several strains of methylobacterium were tested for their ability to produce n-acyl-homoserine lactone (ahl) sig ... | 2007 | 18051753 |
| mesaconyl-coenzyme a hydratase, a new enzyme of two central carbon metabolic pathways in bacteria. | the coenzyme a (coa)-activated c5-dicarboxylic acids mesaconyl-coa and beta-methylmalyl-coa play roles in two as yet not completely resolved central carbon metabolic pathways in bacteria. first, these compounds are intermediates in the 3-hydroxypropionate cycle for autotrophic co2 fixation in chloroflexus aurantiacus, a phototrophic green nonsulfur bacterium. second, mesaconyl-coa and beta-methylmalyl-coa are intermediates in the ethylmalonyl-coa pathway for acetate assimilation in various bacte ... | 2008 | 18065535 |
| implementation of microarrays for methylobacterium extorquens am1. | microarrays are an important tool for understanding global gene expression changes, and the resulting data sets can be used to direct physiologic and metabolic studies. to take advantage of this technology, 60-mer oligonucleotide microarrays were designed for methylobacterium extorquens am1 to study gene expression changes that occur under differing physiological conditions. the carbon utilization pathways for methanol and succinate have been well characterized, and growth with these substrates ... | 2007 | 18092906 |
| intergeneric coaggregation among drinking water bacteria: evidence of a role for acinetobacter calcoaceticus as a bridging bacterium. | intergeneric coaggregation of drinking water bacteria was tested. acinetobacter calcoaceticus was found not only to autoaggregate but also to coaggregate with four of the five other isolates (burkholderia cepacia, methylobacterium sp., mycobacterium mucogenicum, sphingomonas capsulata, and staphylococcus sp.). in its absence, no coaggregation was found. interactions were lectin-saccharide mediated. the putative bridging function of a. calcoaceticus was evidenced by multispecies biofilm studies, ... | 2008 | 18156333 |
| influence of plant species and environmental conditions on epiphytic and endophytic pink-pigmented facultative methylotrophic bacterial populations associated with field-grown rice cultivars. | the total methylotrophic population associated with rice plants from different cultivars was enumerated at three different stages: vegetative, flowering, and harvesting. the bacterial population in the leaf, rhizosphere soil, endophytic in the stem and roots, and epiphytic in the florets and grains were determined from four rice cultivars, il-mi, nam-pyeoung, o-dae, and dong-jin, sampled from three different field sites. the methylotrophic bacteria isolated on ams media containing 0.5% methanol ... | 2007 | 18156780 |
| [changes of chlorine isotope composition characterize bacterial dehalogenation of dichloromethane]. | fractionation of dichloromethane (dcm) molecules with different chlorine isotopes by aerobic methylobacteria methylobacterium dichloromethanicum dm4 and albibacter nethylovorans dm10; cell-free extract of strain dm4; and transconjugant methylobacterium evtorquens al1/pme 8220, expressing the dcma gene for dcm dehalogenase but unable to grow on dcm, was studied. kinetic indices of dcm isotopomers for chlorine during bacterial dehalogenation and diffusion were compared. a two-step model is propose ... | 2007 | 18173108 |
| methylobacterium iners sp. nov. and methylobacterium aerolatum sp. nov., isolated from air samples in korea. | two bacterial strains isolated from air samples, 5317s-33(t) and 5413s-11(t), were characterized by determining their phenotypic characteristics, cellular fatty acid profiles and phylogenetic positions based on 16s rrna gene sequence analysis. 16s rrna gene sequence analysis showed that these isolates belonged to the genus methylobacterium. strain 5317s-33(t) was most closely related to methylobacterium adhaesivum ar27(t) (97.9% sequence similarity). strain 5413s-11(t) was most closely related t ... | 2008 | 18175690 |
| biodegradation of methyl t-butyl ether by aerobic granules under a cosubstrate condition. | aerobic granules efficient at degrading methyl tert-butyl ether (mtbe) with ethanol as a cosubstrate were successfully developed in a well-mixed sequencing batch reactor (sbr). aerobic granules were first observed about 100 days after reactor startup. treatment efficiency of mtbe in the reactor during stable operation exceeded 99.9%, and effluent mtbe was in the range of 15-50 microg/l. the specific mtbe degradation rate was observed to increase with increasing mtbe initial concentration from 25 ... | 2008 | 18183384 |
| methylotrophic bacteria on the surfaces of field-grown sunflower plants: a biogeographic perspective. | plant-associated methylobacteria of the genus methylobacterium colonize the foliage and roots of embryophytes, living on the volatile compound methanol emitted from the cells of their host organism. in this study we analyzed these surface-dwelling pink-pigmented epiphytes in three contrasting habitats of field-grown sunflower plants (helianthus annuus). using the methanol-ammonium salts agar surface impression method and a polymerase chain reaction (pcr)-based assay, we document the occurrence a ... | 2008 | 18193314 |
| bacterial diversity in synovial fluids of patients with tmd determined by cloning and sequencing analysis of the 16s ribosomal rna gene. | the purpose of this study was to examine the presence of bacteria in synovial fluids from patients with temporomandibular joint disorder (tmd) by polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequencing. | 2008 | 18206402 |
| cultivation-independent characterization of methylobacterium populations in the plant phyllosphere by automated ribosomal intergenic spacer analysis. | bacteria of the genus methylobacterium are widespread in the environment, but their ecological role in ecosystems, such as the plant phyllosphere, is not very well understood. to gain better insight into the distribution of different methylobacterium species in diverse ecosystems, a rapid and specific cultivation-independent method for detection of these organisms and analysis of their community structure is needed. therefore, 16s rrna gene-targeted primers specific for this genus were designed ... | 2008 | 18263752 |
| adenosyltransferase tailors and delivers coenzyme b12. | the reactivity and relative rarity of most cofactors pose challenges for their delivery to target enzymes. using kinetic analyses, we demonstrate that adenosyltransferase, which catalyzes the final step in the assimilation of coenzyme b12, directly transfers the cofactor to methylmalonyl coenzyme a mutase. the strategy of using the final enzyme in an assimilation pathway for tailoring a cofactor and delivering it to a dependent enzyme may be general for cofactor trafficking. | 2008 | 18264093 |
| protein oxidation: key to bacterial desiccation resistance? | for extremely ionizing radiation-resistant bacteria, survival has been attributed to protection of proteins from oxidative damage during irradiation, with the result that repair systems survive and function with far greater efficiency during recovery than in sensitive bacteria. here we examined the relationship between survival of dry-climate soil bacteria and the level of cellular protein oxidation induced by desiccation. bacteria were isolated from surface soils of the shrub-steppe of the us d ... | 2008 | 18273068 |
| delivery of tailor-made cobalamin to methylmalonyl-coa mutase. | | 2008 | 18277972 |
| nmr hyperfine shifts in blue copper proteins: a quantum chemical investigation. | we present the results of the first quantum chemical investigations of 1h nmr hyperfine shifts in the blue copper proteins (bcps): amicyanin, azurin, pseudoazurin, plastocyanin, stellacyanin, and rusticyanin. we find that very large structural models that incorporate extensive hydrogen bond networks, as well as geometry optimization, are required to reproduce the experimental nmr hyperfine shift results, the best theory vs experiment predictions having r2 = 0.94, a slope = 1.01, and a sd = 40.5 ... | 2008 | 18314973 |
| colonization pattern of plant root and leaf surfaces visualized by use of green-fluorescent-marked strain of methylobacterium suomiense and its persistence in rhizosphere. | the localization of bacterial cell, pattern of colonization, and survival of methylobacterium suomiense cbmb120 in the rhizosphere of rice and tomato plants were followed by confocal laser scanning, scanning electron microscopy, and selective plating. m. suomiense cbmb120 was tagged with green fluorescent protein (gfp), and inoculation was carried out through seed source. the results clearly showed that the gfp marker is stably inherited and is expressed in planta allowing for easy visualization ... | 2008 | 18320187 |
| thermally treated grass fibers as colonizable substrate for beneficial bacterial inoculum. | this study investigates how thermally treated (i.e., torrefied) grass, a new prospective ingredient of potting soils, is colonized by microorganisms. torrefied grass fibers (tgf) represent a specific colonizable niche, which is potentially useful to establish a beneficial microbial community that improves plant growth. tgf and torrefied grass extracts (tge) were inoculated with a suspension of microorganisms obtained from soil. sequential microbial enrichment steps were then performed in both su ... | 2008 | 18368438 |
| methylobacterium persicinum sp. nov., methylobacterium komagatae sp. nov., methylobacterium brachiatum sp. nov., methylobacterium tardum sp. nov. and methylobacterium gregans sp. nov., isolated from freshwater. | eight strains, 002-165t, 002-079t, b0021t, hojyo2, rb603b, rb677t, 002-074t and rb678, isolated from the environment of food-processing factories in japan, were characterized using a polyphasic approach. the isolates were gram-negative, strictly aerobic, pink-pigmented, facultatively methylotrophic, non-spore-forming rods. the chemotaxonomic characteristics of these isolates included the presence of c18 : 1omega7c as the major cellular fatty acid and ubiquinone q-10 as the predominant ubiquinone ... | 2008 | 18450702 |
| reuse of low concentrated electronic wastewater using selected microbe immobilised cell system. | this work describes a novel technology for the reuse of low concentrated electronic wastewater using selected microbe immobilisation cell (smic) system. the smic system is an innovative technology to maximise the activity of specific microorganisms capable of decomposing tetramethyl ammonium hydroxide (tmah) as a major organic compound in the low concentrated electronic wastewater. the versatility of the smic system has been studied by using continuous-flow reactors. the toc in a smic system was ... | 2008 | 18475012 |
| formate as the main branch point for methylotrophic metabolism in methylobacterium extorquens am1. | in serine cycle methylotrophs, methylene tetrahydrofolate (h4f) is the entry point of reduced one-carbon compounds into the serine cycle for carbon assimilation during methylotrophic metabolism. in these bacteria, two routes are possible for generating methylene h4f from formaldehyde during methylotrophic growth: one involving the reaction of formaldehyde with h4f to generate methylene h4f and the other involving conversion of formaldehyde to formate via methylene tetrahydromethanopterin-depende ... | 2008 | 18502865 |
| culture-independent assessment of rhizobiales-related alphaproteobacteria and the diversity of methylobacterium in the rhizosphere and rhizoplane of transgenic eucalyptus. | the rhizosphere is an ecosystem exploited by a variety of organisms involved in plant health and environmental sustainability. abiotic factors influence microorganism-plant interactions, but the microbial community is also affected by expression of heterologous genes from host plants. in the present work, we assessed the community shifts of alphaproteobacteria phylogenetically related to the rhizobiales order (rhizobiales-like community) in rhizoplane and rhizosphere soils of wild-type and trans ... | 2009 | 18536862 |
| removal of phytotoxic compounds from torrefied grass fibres by plant-beneficial microorganisms. | we aimed to select microorganisms colonizing torrefied grass fibres (tgf) and simultaneously reducing the phytotoxicity which appeared after heat treatment of the fibres. eighty-eight bacterial strains and one fungus, previously isolated from a sequential enrichment experiment on torrefied fibres and extracts, were tested separately for their capacity to decrease phytotoxicity. eleven of the bacterial strains and the fungus significantly reduced phytotoxicity. these organisms were checked for th ... | 2008 | 18537835 |
| bacterial communities in the initial stage of marine biofilm formation on artificial surfaces. | succession of bacterial communities during the first 36 h of biofilm formation in coastal water was investigated at 3 approximately 15 h intervals. three kinds of surfaces (i.e., acryl, glass, and steel substratum) were submerged in situ at sacheon harbor, korea. biofilms were harvested by scraping the surfaces, and the compositions of bacterial communities were analyzed by terminal restriction fragment length polymorphism (t-rflp), and cloning and sequencing of 16s rrna genes. while community s ... | 2008 | 18545967 |
| production of an insecticidal crystal protein from bacillus thuringiensis by the methylotroph methylobacterium extorquens. | the cry1aa protein from bacillus thuringiensis is an insecticidal protein that is highly active against several species of lepidoptera. we cloned and expressed the cry1aa gene in a plant-colonizing methylotroph, methylobacterium extorquens, under the control of the strong m. extorquens am1 methanol dehydrogenase promoter, p(mxaf). transmission electron microscopy revealed characteristic bipyramidal intracellular delta-endotoxin crystals similar to the crystalline inclusions formed by b. thuringi ... | 2008 | 18552184 |
| taxonomy of oxalotrophic methylobacterium strains. | most of the oxalotrophic bacteria are facultative methylotrophs and play important ecological roles in soil fertility and cycling of elements. this study gives a detailed picture of the taxonomy and diversity of these bacteria and provides new information about the taxonomical variability within the genus methylobacterium. twelve mesophilic, pink-pigmented, and facultatively methylotrophic oxalate-oxidizing strains were included in this work that had been previously isolated from the soil and so ... | 2008 | 18581089 |
| emergence of cold water strawberry disease of rainbow trout oncorynchus mykiss in england and wales: outbreak investigations and transmission studies. | cold water strawberry disease (cwsd), or red mark syndrome (rms), is a severe dermatitis affecting the rainbow trout oncorynchus mykiss. the condition, which presents as multifocal, raised lesions on the flanks of affected fish, was first diagnosed in scotland in 2003 and has since spread to england and wales. results of field investigations indicated the condition had an infectious aetiology, with outbreaks in england linked to movements of live fish from affected sites in scotland. transmissio ... | 2008 | 18589997 |
| production of a high viscosity polysaccharide, methylan, in a novel bioreactor. | the effect of shear stress on the production of a high viscosity polysaccharide, methylan, from methanol by methylobacterium organophilum was investigated by using a multidisk mixer. it was observed in the multidisk mixer with defined shear stresses that the specific production rate of methylan increased gradually with increasing shear stress up to 30 pa, and the production rate was constant beyond 30 pa. this result suggested that the limited mass transfer from the medium into cells reduced met ... | 1997 | 18634079 |
| characterization of the gut bacterial community in manduca sexta and effect of antibiotics on bacterial diversity and nematode reproduction. | the tobacco hornworm, manduca sexta, is a model lepidopteran insect used to study the pathogenic and mutualistic phases of entomopathogenic nematodes (epns) and their bacterial symbionts. while intestinal microbial communities could potentially compete with the epn and its bacterial partner for nutrient resources of the insect, the microbial gut community had not been characterized previously. here, we show that the midgut of m. sexta raised on an artificial diet contained mostly gram-positive c ... | 2008 | 18647359 |
| assessment of inhibitory effects of fluoride-coated tubes on biofilm formation by using the in vitro dental unit waterline biofilm model. | this study aimed to establish an in vitro model to simulate biofilms formed in dental unit waterlines (duwls) and to investigate the ability of polyvinylidene fluoride (pvdf)-coated tubes to inhibit biofilm formation using this model. the water and biofilm samples were obtained from duwls which had been clinically used for 2.5 years, and the predominant bacteria were identified. a conventional polyurethane tube was incubated for 24 to 96 h in the mixed flora of isolated bacteria, and the optimal ... | 2008 | 18676694 |
| comprehensive proteomics of methylobacterium extorquens am1 metabolism under single carbon and nonmethylotrophic conditions. | in order to validate a gel free quantitative proteomics assay for the model methylotrophic bacterium methylobacterium extorquens am1, we examined the m. extorquens am1 proteome under single carbon (methanol) and multicarbon (succinate) growth, conditions that have been studied for decades and for which extensive corroborative data have been compiled. in total, 4447 proteins from a database containing 7556 putative orfs from m. extorquens am1 could be identified with two or more peptide sequences ... | 2008 | 18686303 |
| quantitative metabolome analysis using liquid chromatography-high-resolution mass spectrometry. | in this report, we introduce a liquid chromatography single-mass spectrometry method for metabolome quantification, using the ltq orbitrap high-resolution mass spectrometer. analytes were separated with hydrophilic interaction liquid chromatography. at a working resolution of 30,000 (at m/z 400), the limit of detection varied from 50 fmol to 5 pmol for 25 metabolites tested. in terms of metabolite concentration, the linearity was about 2 to 3 orders of magnitude for most compounds (r(2)>0.99). t ... | 2008 | 18694716 |
| diversity of endophytic bacteria from eucalyptus species seeds and colonization of seedlings by pantoea agglomerans. | the diversity and beneficial characteristics of endophytic microorganisms have been studied in several host plants. however, information regarding naturally occurring seed-associated endophytes and vertical transmission among different life-history stages of hosts is limited. endophytic bacteria were isolated from seeds and seedlings of 10 eucalyptus species and two hybrids. the results showed that endophytic bacteria, such as bacillus, enterococcus, paenibacillus and methylobacterium, are verti ... | 2008 | 18710397 |
| development of a broad-host-range sacb-based vector for unmarked allelic exchange. | although genome sequences are available for an ever-increasing number of bacterial species, the availability of facile genetic tools for physiological analysis have generally lagged substantially behind traditional genetic models. | 2008 | 18710539 |
| towards kinetic modeling of global metabolic networks: methylobacterium extorquens am1 growth as validation. | here we report a systematic method for constructing a large scale kinetic metabolic model and its initial application to the modeling of central metabolism of methylobacterium extorquens am1, a methylotrophic and environmental important bacterium. its central metabolic network includes formaldehyde metabolism, serine cycle, citric acid cycle, pentose phosphate pathway, gluconeogensis, phb synthesis and acetyl-coa conversion pathway, respiration and energy metabolism. through a systematic and con ... | 2008 | 18807980 |
| [postoperative intraorbital haematoma with septicemia due to methylbacterium mesophilicum: a rare cause. case report]. | compressive-orbital haematoma is a rare, but dreaded, complication in facial traumatology. | 2008 | 18835613 |
| dual augmentation for aerobic bioremediation of mtbe and tce pollution in heavy metal-contaminated soil. | in this work we isolated from soil and characterized several bacterial strains capable of either resisting high concentrations of heavy metals (cd(2+) or hg(2+) or pb(2+)) or degrading the common soil and groundwater pollutants mtbe (methyl-tert-butyl ether) or tce (trichloroethylene). we then used soil microcosms exposed to mtbe (50 mg/l) or tce (50 mg/l) in the presence of one heavy metal (cd 10 ppm or hg 5 ppm or pb 50 or 100 ppm) and two bacterial isolates at a time, a degrader plus a metal- ... | 2009 | 18987783 |
| statistical media optimization for growth and phb production from methanol by a methylotrophic bacterium. | media components were optimized by statistical design for cell growth and phb production of methylobacterium extorquens dsmz 1340. four important components of growth media were optimized by central composite design. the growth increased from an od=1.35 for choi medium as control to an od=2.15 for optimal medium. then media components for phb production were optimized. optimization of five important factors was conducted by response surface method. the optimal composition of phb production mediu ... | 2009 | 19121581 |
| methylobacterium phyllosphaerae sp. nov., a pink-pigmented, facultative methylotroph from the phyllosphere of rice. | a pink-pigmented, aerobic, facultatively methylotrophic bacterial strain, cbmb27t, isolated from leaf tissues of rice (oryza sativa l. 'dong-jin'), was analysed using a polyphasic taxonomic approach. comparative 16s rrna gene sequence-based phylogenetic analysis placed the strain in a clade with the species methylobacterium oryzae, methylobacterium fujisawaense and methylobacterium mesophilicum; strain cbmb27t showed sequence similarities of 98.3, 98.5 and 97.3 %, respectively, to the type strai ... | 2009 | 19126717 |
| [effect of operational modes on community structure of type i methanotroph in the cover soil of municipal solid waste landfill]. | type i methanotroph is crucial for methane oxidization and it responses fast to the changes in environment. in this study, 16s rdna-based denaturing gradient gel electrophoresis (dgge) gene fingerprint technology was applied to investigate the effect of operational modes, i. e. high-density polyethylene liner (hdpe) isolation or subsurface irrigation of landfill leachate and vegetation, on community structure and diversity of type i methanotroph in soils covering municipal solid waste landfill. ... | 2008 | 19143406 |
| root nodule bacteria isolated from south african lotononis bainesii, l. listii and l. solitudinis are species of methylobacterium that are unable to utilize methanol. | the south african legumes lotononis bainesii, l. listii and l. solitudinis are specifically nodulated by highly effective, pink-pigmented bacteria that are most closely related to methylobacterium nodulans on the basis of 16s rrna gene homology. methylobacterium spp. are characterized by their ability to utilize methanol and other c(1) compounds, but 11 lotononis isolates neither grew on methanol as a sole carbon source nor were able to metabolize it. no product was obtained for pcr amplificatio ... | 2009 | 19152052 |
| [beta-proteorhizobia and nonrhizobial species--a review]. | the terms alpha- and beta-rhizobia were proposed to distinguish the rhizobial alpha- and beta-proteorhizobia respectively. in this review we present recently development of rhizobial taxonomy about beta-rhizobia in the genus burkholeria, cupriavidus isolated from mimosa and alpha-proteorhizobia belonging to methylobacterium, devosia, ochrobactrum, phyllobacterium and blastobacter lineage. furthermore, we also introduced the evolution of symbiosis related genes such as noda and nifh, and posed so ... | 2008 | 19160827 |
| thiosulfate oxidation and mixotrophic growth of methylobacterium goesingense and methylobacterium fujisawaense. | the mixotrophic growth with methanol plus thiosulfate was examined in nutrient-limited mixotrophic condition for methylobacterium goesingense cbmb5 and methylobacterium fujisawaense cbmb37. thiosulfate oxidation increased the growth and protein yield in mixotrophic medium that contained 150 mm methanol and 20 mm sodium thiosulfate, at 144 h. respirometric study revealed that thiosulfate was the most preferable reduced inorganic sulfur source, followed by sulfite and sulfur. m. goesingense cbmb5 ... | 2009 | 19190404 |
| sigma factor mimicry involved in regulation of general stress response. | bacteria have evolved regulatory traits to rapidly adapt to changing conditions. two principal regulatory mechanisms to modulate gene expression consist of regulation via alternative sigma factors and phosphorylation-dependent response regulators. phyr represents a recently discovered protein family combining parts of both systems: a sigma factor-like domain of the extracytoplasmic function (ecf) subfamily linked to a receiver domain of a response regulator. here we investigated the mode of acti ... | 2009 | 19218445 |
| brenneria salicis, the bacterium causing watermark disease in willow, resides as an endophyte in wood. | brenneria salicis has been studied in willow wood only in relation to watermark disease. in this pathogenic condition, the bacterium occurs at high concentrations. pathogenicity of b. salicis is still uncontrollable and the disease unpredictable because the plant-bacteria interaction is not understood. thanks to molecular techniques b. salicis can be detected at low concentrations, which are found in most non-pathogenic interactions. brenneria salicis was identified and traced with a new specifi ... | 2009 | 19220404 |
| demonstration of the ethylmalonyl-coa pathway by using 13c metabolomics. | the assimilation of one-carbon (c1) compounds, such as methanol, by serine cycle methylotrophs requires the continuous regeneration of glyoxylate. instead of the glyoxylate cycle, this process is achieved by a not yet established pathway where coa thioesters are known to play a key role. we applied state-of-the-art metabolomics and (13)c metabolomics strategies to demonstrate how glyoxylate is generated during methylotrophic growth in the isocitrate lyase-negative methylotroph methylobacterium e ... | 2009 | 19261854 |
| enhancing production of l-serine by increasing the glya gene expression in methylobacterium sp. mb200. | microbial fermentation using methylotrophic bacteria is one of the most promising methods for l-serine production. here we describe the metabolic engineering of a methylobacterium strain to increase the production of l-serine. the glya gene, encoding serine hydroxymethyltransferase (shmt), was isolated from the genomic dna of methylobacterium sp. mb200, using a dna fragment encoding methylobacterium extorquens am1 shmt as a probe, and inserted into the vector plafr3. the resulting construct was ... | 2010 | 19266321 |
| liquid chromatography-tandem quadrupole mass spectrometry and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry measurement of targeted metabolites of methylobacterium extorquens am1 grown on two different carbon sources. | complementary methods using liquid chromatography-tandem quadrupole mass spectrometry (lc-ms/ms) and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (gcxgc-tof-ms) were developed and applied to determine targeted metabolites involved in central carbon metabolism [including tricarboxylic acid cycle, serine cycle, ethylmalonyl-coenzyme a (ethylmalonyl-coa) pathway and poly-beta-hydroxybutyrate cycle] of the bacterium methylobacterium extorquens am1 grown on two ca ... | 2009 | 19268957 |
| identification and cloning of a gene encoding dichloromethane dehalogenase from a methylotrophic bacterium, bacillus circulans wz-12 cctcc m 207006. | the gene dehala encoding a novel dichloromethane dehalogenases (dehala), has been cloned from bacillus circulans wz-12 cctcc m 207006. the open reading frame of dehala, spanning 864 bp, encoded a 288-amino acid protein that showed 85.76% identity to the dichloromethane dehalogenases of hyphomicrobium sp. gj21 with several commonly conserved sequences. these sequences could not be found in putative dichloromethane (dcm) dehalogenases reported from other bacteria and fungi. dehala was expressed in ... | 2009 | 19277720 |
| interactions of plant-beneficial bacteria with the ascomycete coniochaeta ligniaria. | to assess the interactions between coniochaeta ligniaria f/tgf15 obtained from torrefied grass fibers (tgf) and selected bacteria from the same substrate. | 2009 | 19298515 |
| methylobacterium sp. resides in unculturable state in potato tissues in vitro and becomes culturable after induction by pseudomonas fluorescens imgb163. | to induce growth of endophytic bacteria residing in an unculturable state in tissues of in vitro-grown potato plantlets. to isolate and identify the induced bacteria and to localize the strains in tissues of in vitro-grown potato plantlets. | 2009 | 19302097 |
| increasing the accuracy of mass isotopomer analysis through calibration curves constructed using biologically synthesized compounds. | mass isotopomer analysis is an important technique to measure the production and flow of metabolites in living cells, tissues, and organisms. this technique depends on accurate quantifications of different mass isotopomers using mass spectrometry. constructing calibration curves using standard samples is the most universal approach to convert raw mass spectrometry measurements into quantitative distributions of mass isotopomers. calibration curve approach has been, however, of very limited use i ... | 2009 | 19370770 |
| population heterogeneity in methylobacterium extorquens am1. | heterogeneity of cells within exponentially growing populations was addressed in a bacterium, the facultative methylotroph methylobacterium extorquens am1. a transcriptional fusion between a well-characterized methanol-inducible promoter (p(mxaf)) and gfp(uv) was used with flow cytometry to analyse the distribution of gene expression in populations grown on either succinate or methanol, correlated with forward scatter as a measure of cell size. these cell populations were found to consist of thr ... | 2009 | 19383691 |
| a rotary mechanism for coenzyme b(12) synthesis by adenosyltransferase. | adenosyltransferases (atrs) catalyze the synthesis of the reactive cobalt-carbon bond found in coenzyme b(12) or 5'-deoxyadenosylcobalamin (adocbl), which serves as a cofactor for a number of isomerases. the reaction involves a reductive adenosylation of cob(ii)alamin in which an electron delivered by a reductase reduces cob(ii)alamin to cob(i)alamin, which attacks the 5'-carbon of atp to form adocbl and inorganic triphosphate. of the three classes of atrs found in nature, the pduo type, which i ... | 2009 | 19413290 |
| methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of c1 compounds from natural and industrial sources. | methylotrophy describes the ability of organisms to grow on reduced organic compounds without carbon-carbon bonds. the genomes of two pink-pigmented facultative methylotrophic bacteria of the alpha-proteobacterial genus methylobacterium, the reference species methylobacterium extorquens strain am1 and the dichloromethane-degrading strain dm4, were compared. | 2009 | 19440302 |
| plant-associated methylobacteria as co-evolved phytosymbionts: a hypothesis. | due to their wall-associated pectin metabolism, growing plant cells emit significant amounts of the one-carbon alcohol methanol. pink-pigmented microbes of the genus methylobacterium that colonize the surfaces of leaves (epiphytes) are capable of growth on this volatile c1-compound as sole source of carbon and energy. in this article the results of experiments with germ-free (gnotobiotic) sporophytes of angiosperms (sunflower, maize) and gametophytes of bryophytes (a moss and two liverwort speci ... | 2007 | 19516971 |
| biodegradation of haloacetic acids by bacterial isolates and enrichment cultures from drinking water systems. | biodegradation is a potentially important loss process for haloacetic acids (haas), a class of chlorination byproducts, in water treatment and distribution systems, but little is known about the organisms involved (i.e., identity, substrate range, biodegradation kinetics). in this research, 10 biomass samples (i.e., tap water, distribution system biofilms, and prechlorinated granular activated carbon filters) from nine drinking water systems were used to inoculate a total of thirty enrichment cu ... | 2009 | 19534130 |
| asymmetric, bimodal trade-offs during adaptation of methylobacterium to distinct growth substrates. | trade-offs between selected and nonselected environments are often assumed to exist during adaptation. this phenomenon is prevalent in microbial metabolism, where many organisms have come to specialize on a narrow breadth of substrates. one well-studied example is methylotrophic bacteria that can use single-carbon (c(1)) compounds as their sole source of carbon and energy, but generally use few, if any, multi-c compounds. here, we use adaptation of experimental populations of the model methylotr ... | 2009 | 19545267 |
| species-specific structural and functional diversity of bacterial communities in lichen symbioses. | lichens are generally considered as mutualisms between fungi and green algae or cyanobacteria. these partnerships allow light-exposed and long-living joint structures. the unique organization of lichens provides still unexplored environments for microbial communities. to study lichen-associated bacterial communities, we analyze samples, by a polyphasic approach, from three lichen species (cladonia arbuscula, lecanora polytropa and umbilicaria cylindrica) from alpine environments. our results ind ... | 2009 | 19554038 |
| the phyr-sigma(ecfg) signalling cascade is involved in stress response and symbiotic efficiency in bradyrhizobium japonicum. | phyr is an unusual type of response regulator consisting of a receiver domain and an extracytoplasmic function (ecf) sigma factor-like domain. it was recently described as a master regulator of general stress response in methylobacterium extorquens. orthologues of this regulator are present in essentially all free-living alphaproteobacteria. in most of them, phyr is genetically closely linked to a gene encoding an ecf sigma factor. here, we investigate the role of these two regulators in the soy ... | 2009 | 19555458 |
| methylovirgula ligni gen. nov., sp. nov., an obligately acidophilic, facultatively methylotrophic bacterium with a highly divergent mxaf gene. | two strains of gram-negative, aerobic, non-pigmented, non-motile, rod-shaped bacteria were isolated from beechwood blocks during decay by the white-rot fungus hypholoma fasciculare and were designated strains bw863(t) and bw872. they are capable of methylotrophic growth and assimilate carbon via the ribulose-bisphosphate pathway. in addition to methanol, the novel isolates utilized ethanol, pyruvate and malate. strains bw863(t) and bw872 are obligately acidophilic, mesophilic organisms capable o ... | 2009 | 19622650 |
| enterobacter arachidis sp. nov., a plant-growth-promoting diazotrophic bacterium isolated from rhizosphere soil of groundnut. | a methylotrophic nitrogen-fixing bacterial strain, ah-143(t), isolated from the rhizosphere soil of field-grown groundnut was analysed by a polyphasic taxonomic approach. comparative 16s rrna gene sequence analysis combined with rpob gene sequence analysis allocated strain ah-143(t) to the family enterobacteriaceae, with enterobacter radicincitans and enterobacter cowanii as the closest relatives. the strain is gram-stain-negative, non-spore-forming, aerobic and motile, having straight rod-shape ... | 2010 | 19684326 |
| (2s)-methylsuccinyl-coa dehydrogenase closes the ethylmalonyl-coa pathway for acetyl-coa assimilation. | many organic substrates are metabolized via acetyl-coenzyme a (coa) and enter central carbon metabolism at the level of this compound. we recently described the outlines of the ethylmalonyl-coa pathway, a new acetyl-coa assimilation strategy that operates in a number of bacteria such as rhodobacter sphaeroides, methylobacterium extorquens and streptomycetes and replaces the glyoxylate cycle. this new pathway involves a unique central reaction sequence catalysed by characteristic enzymes. here, w ... | 2009 | 19703103 |
| plant colonization by pink-pigmented facultative methylotrophic bacteria (ppfms). | bacteria belonging to the genus methylobacterium are characterized by being able to rely on methanol as a sole carbon and energy source and by presenting a more or less intense pink reddish pigmentation. these bacteria, also referred to as pink-pigmented methylotrophic bacteria (ppfms), are common inhabitants of the phyllosphere and are found in many other environmental samples. since they grow slowly they are often overlooked and their impact on phyllosphere microbial communities and on the pla ... | 2004 | 19712320 |
| molecular phylogeny of methylotrophs in a deep-sea sediment from a tropical west pacific warm pool. | the presence and phylogeny of methylotrophs, including methanotrophs, in a deep-sea sediment of a tropical west pacific warm pool site wp was investigated by molecular marker-based analysis of mxaf, pmoa and archaeal 16s rrna genes. mxaf amino acid sequence analysis revealed that microbes belonging to the alpha-proteobacteria and most related to hyphomicrobium and methylobacterium were the dominant aerobic methylotrophs in this deep-sea sediment; also, a small percentage of type ii methanotrophs ... | 2004 | 19712348 |
| fast growth increases the selective advantage of a mutation arising recurrently during evolution under metal limitation. | understanding the evolution of biological systems requires untangling the molecular mechanisms that connect genetic and environmental variations to their physiological consequences. metal limitation across many environments, ranging from pathogens in the human body to phytoplankton in the oceans, imposes strong selection for improved metal acquisition systems. in this study, we uncovered the genetic and physiological basis of adaptation to metal limitation using experimental populations of methy ... | 2009 | 19763169 |
| transmission of methylobacterium mesophilicum by bucephalogonia xanthophis for paratransgenic control strategy of citrus variegated chlorosis. | methylobacterium mesophilicum, originally isolated as an endophytic bacterium from citrus plants, was genetically transformed to express green fluorescent protein (gfp). the gfp-labeled strain of m. mesophilicum was inoculated into catharanthus roseus (model plant) seedlings and further observed colonizing its xylem vessels. the transmission of this endophyte by bucephalogonia xanthophis, one of the insect vectors that transmit xylella fastidiosa subsp. pauca, was verified by insects feeding fro ... | 2009 | 19763419 |
| [methylobacterium radiotolerans bacteremia in hemodialysis patients]. | central venous catheters (cvcs) play an important role in replacement therapy for patients with acute and chronic renal failure. secondary infections due to central venous access are responsible for 48-73% of bacteremia in hemodialysis patients and are an important cause of morbidity and increased health costs for these patients. episodes of unexplained fever were noted in hemodialysis patients in our center starting in october 2006. an investigation for causative microorganisms was conducted fr ... | 2009 | 19802807 |
| community proteogenomics reveals insights into the physiology of phyllosphere bacteria. | aerial plant surfaces represent the largest biological interface on earth and provide essential services as sites of carbon dioxide fixation, molecular oxygen release, and primary biomass production. rather than existing as axenic organisms, plants are colonized by microorganisms that affect both their health and growth. to gain insight into the physiology of phyllosphere bacteria under in situ conditions, we performed a culture-independent analysis of the microbiota associated with leaves of so ... | 2009 | 19805315 |
| characterization of an isoproturon mineralizing bacterial culture enriched from a french agricultural soil. | the phenylurea herbicide isoproturon, 3-(4-isopropylphenyl)-1,1-dimethylurea (ipu), was found to be rapidly mineralized by a bacterial culture isolated from an agricultural soil regularly exposed to ipu. molecular analysis of the bacterial culture by dna fingerprinting, cloning and sequencing of the 16s rrna genes revealed that it consisted of six different members among whom the dominant was related to sphingomonas sp. six bacterial strains belonging to genera ancylobacter, pseudomonas, stenotr ... | 2009 | 19836052 |
| icmf is a fusion between the radical b12 enzyme isobutyryl-coa mutase and its g-protein chaperone. | coenzyme b(12) is used by two highly similar radical enzymes, which catalyze carbon skeleton rearrangements, methylmalonyl-coa mutase and isobutyryl-coa mutase (icm). icm catalyzes the reversible interconversion of isobutyryl-coa and n-butyryl-coa and exists as a heterotetramer. in this study, we have identified >70 bacterial proteins, which represent fusions between the subunits of icm and a p-loop gtpase and are currently misannotated as methylmalonyl-coa mutases. we designate this fusion prot ... | 2010 | 19864421 |
| metabolite profiling uncovers plasmid-induced cobalt limitation under methylotrophic growth conditions. | the introduction and maintenance of plasmids in cells is often associated with a reduction of growth rate. the reason for this growth reduction is unclear in many cases. | 2009 | 19915676 |
| long-chain acyl-homoserine lactones from methylobacterium mesophilicum: synthesis and absolute configuration. | the acyl-homoserine lactones (acyl-hsls) produced by methylobacterium mesophilicum isolated from orange trees infected with the citrus variegated chlorosis (cvc) disease have been studied, revealing the occurrence of six long-chain acyl-hsls, i.e., the saturated homologues (s)-n-dodecanoyl (1) and (s)-n-tetradecanoyl-hsl (5), the uncommon odd-chain n-tridecanoyl-hsl (3), the new natural product (s)-n-(2e)-dodecenoyl-hsl (2), and the rare unsaturated homologues (s)-n-(7z)-tetradecenoyl (4) and (s ... | 2009 | 19919062 |
| community shifts of soybean stem-associated bacteria responding to different nodulation phenotypes and n levels. | the diversity of stem-associated bacteria of non-nodulated (nod(-)), wild-type nodulated (nod(+)) and hypernodulated (nod(++)) soybeans were evaluated by clone library analyses of the 16s ribosomal rna gene. soybeans were dressed with standard nitrogen (sn) fertilization (15 kg n ha(-1)) and heavy nitrogen (hn) fertilization (615 kg n ha(-1)). the relative abundance of alphaproteobacteria in nod(+) soybeans (66%) was smaller than that in nod(-) and nod(++) soybeans (75-76%) under sn fertilizatio ... | 2010 | 19924157 |
| [anti-alcoholic and anti-narcotic action of methylobacteriim extorquens ucm b-3368]. | the paper deals with action efficiency of microbial biomass on characteristic indicators at alcohol and morphine organism intoxication. the investigated microbial biomass affects the regulatory biochemical and physiological systems in experimental animals, normalizes activity of alcohol dehydrogenase and aldehide dehydrogenase, as well as the content of dophamine, disturbed under the effect of alcohol and morphine. thus, the organism intoxication decreases. except for the specific action, the ab ... | 2009 | 19938614 |
| expanding the family of collagen proteins: recombinant bacterial collagens of varying composition form triple-helices of similar stability. | the presence of the (gly-xaa-yaa)(n) open reading frames in different bacteria predicts the existence of an expanded family of collagen-like proteins. to further explore the triple-helix motif and stabilization mechanisms in the absence of hydroxyproline (hyp), predicted novel collagen-like proteins from gram-positive and -negative bacteria were expressed in escherichia coli and characterized. soluble proteins capable of successful folding and in vitro refolding were observed for collagen protei ... | 2010 | 20025291 |
| enrichment and cultivation of pelagic bacteria from a humic lake using phenol and humic matter additions. | abstract individual bacterial populations are known to respond differently toward substrate availability. to test how the availability of either pure phenol or natural humic matter (hm) selects for specific pelagic bacteria phylotypes from a humic lake (lake grosse fuchskuhle, northeastern germany), we used culture-dependent and -independent approaches. using a batch approach, the bacterial community composition (bcc) differed depending on both the quantity and the quality of added substrates. u ... | 2010 | 20100184 |
| alternative route for glyoxylate consumption during growth on two-carbon compounds by methylobacterium extorquens am1. | methylobacterium extorquens am1 is a facultative methylotroph capable of growth on both single-carbon and multicarbon compounds. mutants defective in a pathway involved in converting acetyl-coenzyme a (coa) to glyoxylate (the ethylmalonyl-coa pathway) are unable to grow on both c(1) and c(2) compounds, showing that both modes of growth have this pathway in common. however, growth on c(2) compounds via the ethylmalonyl-coa pathway should require glyoxylate consumption via malate synthase, but a m ... | 2010 | 20118267 |
| methylobacterium dankookense sp. nov., isolated from drinking water. | a pink-pigmented bacterium, designated sw08-7(t) was isolated from the drinking water of a water purifier. cells were gram-negative, rod-shaped, strictly aerobic, and non-spore-forming. it grew optimally at 25 degrees c, ph 6 approximately 7. phylogenese analysis based on 16s rrna gene sequence showed that strain sw08-7(t) belongs to the genus methylobacterium. the highest 16s rrna gene sequence similarities were found to methylobacterium mesophilicum jcm 2829(t) (96.9%), methylobacterium brachi ... | 2010 | 20127465 |
| dual control of sinorhizobium meliloti rpoe2 sigma factor activity by two phyr-type two-component response regulators. | rpoe2 is an extracytoplasmic function (ecf) sigma factor involved in the general stress response of sinorhizobium meliloti, the nitrogen-fixing symbiont of the legume plant alfalfa. rpoe2 orthologues are widely found among alphaproteobacteria, where they play various roles in stress resistance and/or host colonization. in this paper, we report a genetic and biochemical investigation of the mechanisms of signal transduction leading to s. meliloti rpoe2 activation in response to stress. we showed ... | 2010 | 20154128 |
| site and plant species are important determinants of the methylobacterium community composition in the plant phyllosphere. | the plant phyllosphere constitutes a habitat for numerous microorganisms; among them are members of the genus methylobacterium. owing to the ubiquitous occurrence of methylobacteria on plant leaves, they represent a suitable target for studying plant colonization patterns. the influence of the factor site, host plant species, time and the presence of other phyllosphere bacteria on methylobacterium community composition and population size were evaluated in this study. leaf samples were collected ... | 2010 | 20164863 |
| a joint x-ray and neutron study on amicyanin reveals the role of protein dynamics in electron transfer. | the joint x-ray/neutron diffraction model of the type i copper protein, amicyanin from paracoccus denitrificans was determined at 1.8 a resolution. the protein was crystallized using reagents prepared in d(2)o. about 86% of the amide hydrogen atoms are either partially or fully exchanged, which correlates well with the atomic depth of the amide nitrogen atom and the secondary structure type, but with notable exceptions. each of the four residues that provide copper ligands is partially deuterate ... | 2010 | 20351252 |
| effect of bacterial inoculation, plant genotype and developmental stage on root-associated and endophytic bacterial communities in potato (solanum tuberosum). | beneficial bacteria interact with plants by colonizing the rhizosphere and roots followed by further spread through the inner tissues, resulting in endophytic colonization. the major factors contributing to these interactions are not always well understood for most bacterial and plant species. it is believed that specific bacterial functions are required for plant colonization, but also from the plant side specific features are needed, such as plant genotype (cultivar) and developmental stage. v ... | 2010 | 20352404 |