| cepacidine a, a novel antifungal antibiotic produced by pseudomonas cepacia. ii. physico-chemical properties and structure elucidation. | cepacidine a is a novel glycopeptide with a potent antifungal activity, which is produced by pseudomonas cepacia af 2001. its molecular weight was determined by fab-ms (m/z 1215). the compound is comprised of glycine (1), serine (2), 2,4-diaminobutyric acid (1), aspartic acid (1), beta-hydroxy tyrosine (1), beta-hydroxy asparagine (1), xylose (1) and 5,7-dihydroxy-3,9-diamino-octadecanoic acid (1). unfortunately, cepacidine a is a mixture of a1 and a2, either of which is barely distinguishable. ... | 1994 | 7531194 |
| improved results of lung transplantation for patients with cystic fibrosis. | patients with cystic fibrosis pose particular challenges for lung transplant surgeons. earlier reports from north american centers suggested that patients with cystic fibrosis were at greater risk for heart-lung or isolated lung transplantation than other patients with end-stage pulmonary disease. during a 3 1/2 year period, 44 patients with end-stage lung disease resulting from cystic fibrosis underwent double lung transplantation at this institution. during the same interval, 18 patients with ... | 1995 | 7531796 |
| cable (cbl) type ii pili of cystic fibrosis-associated burkholderia (pseudomonas) cepacia: nucleotide sequence of the cbla major subunit pilin gene and novel morphology of the assembled appendage fibers. | previous studies have shown that appendage pili of burkholderia cepacia strains isolated from patients with cystic fibrosis (cf) at the hospital for sick children, toronto, canada, mediate adherence to mucus glycoproteins and also enhance adherence to epithelial cells. the specific pilin-associated adhesin molecule is a 22-kda protein. in the present study we purified the major subunit pilin (17 kda) and immunolocalized it to peritrichously arranged pili. on the basis of their novel morphologica ... | 1995 | 7532166 |
| structurally variant classes of pilus appendage fibers coexpressed from burkholderia (pseudomonas) cepacia. | one or more of five morphologically distinct classes of appendage pili were determined to be peritrichously expressed by burkholderia (formerly pseudomonas) cepacia isolated from disparate sources. b. cepacia-encoded cbla pilin gene hybridization-based analysis revealed that one associated class, cable (cbl) adhesin type iib. cepacia pili, correlates with epidemically transmitted strains from a single cystic fibrosis (cf) center. when only phenotypic assays were available, correlations between t ... | 1995 | 7532167 |
| burkholderia cepacia in cystic fibrosis. | | 1995 | 7532284 |
| burkholderia cepacia in cystic fibrosis. | | 1995 | 7532285 |
| burkholderia cepacia in cystic fibrosis. | | 1995 | 7532286 |
| burkholderia (formerly pseudomonas) cepacia porin is an oligomer composed of two component proteins. | the 81 kda protein (designated opcpo) which forms a diffusion pore in the outer membrane of burkholderia (formerly pseudomonas) cepacia has a unique characteristic in that when the purified protein is heated it yields a major 36 kda protein (designated opcp1) and a minor 27 kda protein (designated opcp2). moreover, incubation of opcpo in citrate buffer at ph 3.0 produced an unusual dissociation into 72 kda and 27 kda proteins. for the characterization of opcpo and its derivatives, opcp1 and opcp ... | 1994 | 7533592 |
| recovery of pseudomonas cepacia and other pseudomonas species from the environment. | nine hundred sixteen cultures were obtained from homes of patients with cystic fibrosis, control homes, salad bars, and food markets, and analyzed for the presence of pseudomonas cepacia and related bacteria. p cepacia was recovered from 5 (18%) of 27 homes, and from 20 (4%) of 509 cultures collected outside of homes. relative to other pseudomonads, p cepacia is found infrequently in the environment. it is not clear how frequently these sources contribute to acquisition of this bacteria by perso ... | 1995 | 7534780 |
| fatal sepsis due to pseudomonas cepacia. | pseudomonas cepacia is known as an opportunistic pathogen in the patients with altered host defence. we report a case of hospital-acquired fatal sepsis due to p. cepacia in a child with no known defect in immune system. virulence of the organism was indicated by the high magnitude of septicaemia. the organism was successfully eliminated by ceftriaxone but the patient died of renal failure. | 1994 | 7535478 |
| comparison by extended ribotyping of pseudomonas cepacia isolated from cystic fibrosis patients with acute and chronic infections. | multiple isolates of pseudomonas cepacia, from two cystic fibrosis (cf) patients who were chronically infected and two others who suffered acute fatal lung infections, were examined by multilocus enzyme electrophoresis and four-enzyme ribotyping. the strains isolated from the fatalities belonged to a clone, electropherotype 12 (et12) that is endemic in the ontario patients' province of origin. et12 strains have also been isolated from outbreaks in cf patients in the united kingdom, where they ar ... | 1994 | 7535673 |
| an outbreak of burkholderia (formerly pseudomonas) cepacia respiratory tract colonization and infection associated with nebulized albuterol therapy. | to investigate an outbreak of burkholderia (formerly pseudomonas) cepacia respiratory tract colonization and infection in mechanically ventilated patients. | 1995 | 7536401 |
| defining potential targets for immunotherapy in burkholderia cepacia infection. | burkholderia cepacia has become a serious source of infection in patients with cystic fibrosis. antibiotic therapy is difficult as the bacteria are intrinsically resistant to most antibiotics. the present study compared the antibody response by immunoblot of 50 negative control sera, 22 patients with cystic fibrosis and no evidence of b. cepacia, 9 clinically well patients with cystic fibrosis colonised by b. cepacia and 5 patients with cystic fibrosis and deteriorating or fatal b. cepacia infec ... | 1995 | 7536517 |
| differences in eucaryotic cell binding of pseudomonas. | the lungs of cystic fibrosis (cf) patients are frequently chronically colonized by pseudomonas aeruginosa. recently there has been an increase in colonization by another pathogen pseudomonas cepacia, which can cause a rapid decline in clinical condition or death of the patient. the nature of the factor(s) which predispose cf patients to colonization by one or both of these opportunistic pathogens is unknown. it has been suggested that the genetic defect in cf patients results in an increase in t ... | 1994 | 7536882 |
| xanthomonas maltophilia misidentified as pseudomonas cepacia in cultures of sputum from patients with cystic fibrosis: a diagnostic pitfall with major clinical implications. | pseudomonas cepacia infection in patients with cystic fibrosis (cf) has major significance in terms of infection control, psychosocial issues, and medical treatment. we describe three instances in which the diagnostic laboratory misidentified xanthomonas maltophilia as p. cepacia in cultures of sputum from patients with cf. these errors were recognized when 3 (9%) of 32 isolates, which had all been identified as p. cepacia and had been submitted to the canadian pseudomonas repository laboratory ... | 1995 | 7537977 |
| sequence analysis of a gene cluster involved in metabolism of 2,4,5-trichlorophenoxyacetic acid by burkholderia cepacia ac1100. | burkholderia cepacia ac1100 utilizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-t) as a sole source of carbon and energy. pt88 is a chromosomal deletion mutant of b. cepacia ac1100 and is unable to grow on 2,4,5-t. the nucleotide sequence of a 5.5-kb chromosomal fragment from b. cepacia ac1100 which complemented pt88 for growth on 2,4,5-t was determined. the sequence revealed the presence of six open reading frames, designated orf1 to orf6. five polypeptides were produced when this dna region was ... | 1995 | 7538273 |
| tom, a new aromatic degradative plasmid from burkholderia (pseudomonas) cepacia g4. | burkholderia (pseudomonas) cepacia pr1(23) has been shown to constitutively express to toluene catabolic pathway distinguished by a unique toluene ortho-monooxygenase (tom). this strain has also been shown to contain two extrachromosomal elements of < 70 and > 100 kb. a derivative strain cured of the largest plasmid, pr1(23) cure, was unable to grow on phenol or toluene as the sole source of carbon and energy, which requires expression of the tom pathway. transfer of the larger plasmid from stra ... | 1995 | 7538275 |
| identification of aquatic burkholderia (pseudomonas) cepacia by hybridization with species-specific rrna gene probes. | burkholderia (pseudomonas) cepacia is a common environmental bacterium which can be pathogenic for plants and humans. in this study, four strategies were used to identify aquatic isolates: api test strips, hybridization with species-specific dna probes for the 16s and 23s rrna genes, fatty acid methyl ester (fame) profiles, and growth on selective medium (tb-t agar [c. hagedorn, w. d. gould, t. r. bardinelli, and d. r. gustarson, appl. environ. microbiol. 53:2265-2268, 1987]). only 59% of the is ... | 1995 | 7538282 |
| cloning and nucleotide sequence comparison of the groe operon of pseudomonas aeruginosa and burkholderia cepacia. | by alignment of groel amino acid sequences from four distantly related bacteria two highly conserved domains were identified. two oligonucleotides complementary to the conserved domains were designed based on the preferred pseudomonas aeruginosa codon usage. the primers were used in the pcr to amplify a 900-base fragment of the p. aeruginosa groel gene. the fragment was sequenced and the partial groel sequence was expanded by vectorette pcr upstream and downstream to cover the complete p. aerugi ... | 1995 | 7538307 |
| serum igg response to burkholderia cepacia outer membrane antigens in cystic fibrosis: assessment of cross-reactivity with pseudomonas aeruginosa. | burkholderia cepacia (pseudomonas cepacia) is now recognised as an important pathogen in cystic fibrosis patients, and several reports have suggested that sputum-culture-proven colonisation occurs despite the presence of specific antibody. in an attempt to establish the use of antibody studies as diagnostic and prognostic indicators of b. cepacia infection, we have examined the igg response to b. cepacia outer membrane proteins and lipopolysaccharide in patients also colonised with p. aeruginosa ... | 1995 | 7539670 |
| burkholderia cepacia and cystic fibrosis: do natural environments present a potential hazard? | an environmental survey of 55 sites yielded only 12 burkholderia cepacia isolates, none of which displayed the phenotypic properties of a multiresistant epidemic strain associated with pulmonary colonization in patients with cystic fibrosis. although the environment probably poses a low risk for patients with cystic fibrosis as a source of b. cepacia, the pathogenic potential of individual environmental strains remains unclear. we advise caution in the development of b. cepacia as a biocontrol a ... | 1995 | 7540623 |
| cloning of a mineral phosphate-solubilizing gene from pseudomonas cepacia. | we have recently shown that the ability of some gram-negative bacteria to dissolve poorly soluble calcium phosphates (mps+ phenotype) is the result of periplasmic oxidation of glucose to gluconic acid via the quinoprotein glucose dehydrogenase (gdh), a component of the direct oxidation pathway. escherichia coli k-12 derivatives synthesize apo-gdh but not the cofactor pyrroloquinoline-quinone (pqq) essential for formation of the holoenzyme. therefore, in the absence of exogenous pqq, these strain ... | 1995 | 7540821 |
| lung disease in the cystic fibrosis mouse exposed to bacterial pathogens. | lung disease is the major cause of death in cystic fibrosis (cf), but there is no evidence for overt lung involvement at birth. we show here that the same is true for the gene targeted cftrm1hgu mutant mouse. furthermore, this cf mouse model demonstrates an impaired capacity to clear staphylococcus aureus and burkholderia (pseudomonas) cepacia, two opportunistic lung pathogens closely associated with lung disease in cf subjects. the cftrm1hgu homozygotes display mucus retention and frank lung di ... | 1995 | 7540910 |
| insertional sequence primers for bordetella pertussis diagnostic polymerase chain reaction differentiate strains of pseudomonas cepacia. | insertion sequence primers originally intended to amplify a singular specific product for the rapid diagnosis of bordetella pertussis respiratory infection were used to differentiate strains of pseudomonas (burkholderia) cepacia. a modified sample preparation of proteinase k treatment and boiling was used in lieu of dna extraction. the method was simple, rapid, and reproducible. this scheme identified 10 variations among 35 strains. repeat strains from patients with cystic fibrosis and epidemiol ... | 1995 | 7541063 |
| [pseudomonas mallei and pseudomonas pseudomallei: introduction and maintenance of natural and recombinant plasmid replicons]. | comparative analysis of recipient activity of pseudomonas mallei, pseudomonas pseudomallei, and pseudomonas cepacia strains towards naturally occurring and recombinant plasmid replicons was carried out. autonomic broad host range vector plasmids based on rsf1010(incq) and psa(incw) replicons as well as integrative vectors based on psup202(co1e1) replicon have been constructed. the study has shown that naturally occurring plasmids rsf1010(incq), psa(incw), r15(incn), and rp4(incp) are being effic ... | 1995 | 7541512 |
| effects of growth rate and nutrient limitation on virulence factor production in burkholderia cepacia. | the influence of growth rate and oxygen availability on siderophore, protease, and lipase production in burkholderia cepacia was assessed for cells grown in a chemostat under iron limitation. whereas siderophore and protease production increased with growth rate and oxygen yet decreased under oxygen depletion, lipase production demonstrated the opposite trend. | 1995 | 7541788 |
| pleural complications in lung transplant recipients. | pleural complications occurred in 30 (22%) of 138 patients after 53 single and 91 double lung transplants between september 1986 and february 1993. these were defined for the purpose of this study as pneumothorax persisting beyond the first 14 postoperative days, recurrent pneumothorax, or any other pleural process that necessitated diagnostic or therapeutic intervention. overall, a higher pleural complication rate was seen in double lung transplantation (25 of 30) than in single lung transplant ... | 1995 | 7541881 |
| bacterial contamination of aerosol solutions containing antibiotics. | in an investigation of microbial contamination of aerosol solutions containing antibiotics (hospital pharmaceutical preparations, no preservatives added), five of six residual solutions after multiple use for 7 days were contaminated at a concentration of 10(6) viable counts/ml. contaminants were glucose-nonfermenting gram-negative bacilli such as pseudomonas cepacia and flavobacterium meningosepticum. the major contaminant, p. cepacia, multiplied rapidly in the aerosol solution under simulated ... | 1995 | 7541888 |
| selective media for isolation of burkholderia (pseudomonas) cepacia from the respiratory secretions of patients with cystic fibrosis. | one hundred and six specimens from 90 patients with cystic fibrosis were evaluated for the presence of burkholderia cepacia using a current routine diagnostic protocol as well as a research protocol involving polymyxin b-macconkey agar without crystal violet, pc agar, ofpvl agar, and a selective brain-heart infusion broth. ten specimens from eight patients (8.9%) were positive by any method. the selective enrichment broth was the only medium that yielded b cepacia from all 10 positive samples, a ... | 1995 | 7543119 |
| structures of the two polymers present in the lipopolysaccharide of burkholderia (pseudomonas) cepacia serogroup o4. | like several other strains of burkholderia (pseudomonas) cepacia, the reference strain for serogroup o4 in the french typing scheme [heidt, a., monteil, h. & richard, c. (1983) j. clin. microbiol. 18, 738-740] produces a lipopolysaccharide containing two distinct polymers. attempts to separate the polymers chromatographically were unsuccessful, but the periodate-resistant major polymer could be isolated by application of the smith degradation technique to the mixture. by means of chemical and nm ... | 1995 | 7544286 |
| bacterial infection in cystic fibrosis (cf): special reference to mycobacteria and burkholderia cepacia. | | 1995 | 7547352 |
| in vitro antibacterial activities of fk037: a new parenteral cephalosporin. | the antibacterial and bactericidal activities and the stability of fk037 to beta-lactamases were investigated and compared with those of cefpirome, flomoxef, ceftazidime, ceftizoxime, and vancomycin. against clinical isolates of methicillin-sensitive and methicillin-resistant staphylococcus aureus, fk037 was less active than vancomycin but more potent than the other drugs tested. among highly methicillin-resistant staphylococci (mic for methicillin: 200 mg/l or higher), none of the strains were ... | 1995 | 7555206 |
| auxotrophy of burkholderia (pseudomonas) cepacia from cystic fibrosis patients. | the nutritional status of 89 isolates of burkholderia cepacia from 81 cystic fibrosis (cf) patients was evaluated. forty of the isolates, from 38 patients, were not able to grow in a minimal medium containing glucose and mineral salts only and were thus auxotrophs. in contrast, all of 29 isolates from non-cf (clinical and environmental) sources were prototrophic. addition of a pool of amino acids to the minimal medium was sufficient to promote growth of all tested cf auxotrophic isolates. indeed ... | 1995 | 7559977 |
| the emergence of epidemic, multiple-antibiotic-resistant stenotrophomonas (xanthomonas) maltophilia and burkholderia (pseudomonas) cepacia. | stenotrophomonas (xanthomonas) maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised cancer patients and transplant recipients. s. maltophilia has been documented as a cause of bacteraemia, infections of the respiratory and urinary tracts, meningitis, serious wound infections, mastoiditis, epididymitis, conjunctivitis and endocarditis. the reservoir of s. maltophilia and the mechanisms by which it is transmitted, remain largely unknown. risk analysis has shown ... | 1995 | 7560984 |
| genetic and molecular analysis of a regulatory region of the herbicide 2,4-dichlorophenoxyacetate catabolic plasmid pjp4. | in alcaligenes eutrophus jmp134, pjp4 carries the genes coding for 2,4-dichlorophenoxyacetate (2,4-d) and 3-chlorobenzoate (3-cba) degradation plus mercury resistance. the plasmid genes specifying 2,4-d and 3-cba catabolism are organized in three operons: tfda, tfdb, and tfdcdef. regulation of these operons by two unlinked genes, tfdr and tfds, has been proposed. physical and dna sequence analyses revealed that the tfdr and tfds genes were identical and were located within a longer inverted repe ... | 1995 | 7565094 |
| [molecular typing by pulsed field gel electrophoresis of pseudomonas (burkholderia) cepacia isolated from a nosocomial infection]. | because of the emergence of pseudomonas (burkholderia) cepacia, isolated among patients from an intensive care unit of the hôpital pellegrin (bordeaux), strains were studied in the aim to determine the source and the mode of transmission of the bacteria. the study was performed on 44 isolates of p. cepacia from 21 patients (23 strains) or from the environment (16 isolates from ventilators and five from the distilled water used for these ventilators). susceptibility to 23 antibiotics of these str ... | 1995 | 7567128 |
| a meta cleavage pathway for 4-chlorobenzoate, an intermediate in the metabolism of 4-chlorobiphenyl by pseudomonas cepacia p166. | bacterial degradation of biphenyl and polychlorinated biphenyls proceeds by a well-studied pathway which produces benzoate and 2-hydroxypent-2,4-dienoate (or, in the case of polychlorinated biphenyls, the chlorinated derivatives of these compounds). pseudomonas cepacia p166 utilizes 4-chlorobiphenyl for growth and produces 4-chlorobenzoate as a central intermediate. in this study we found that strain p166 further transforms 4-chlorobenzoate to 4-chlorocatechol, which is mineralized by a meta cle ... | 1995 | 7574580 |
| induction of toluene oxidation activity in pseudomonas mendocina kr1 and pseudomonas sp. strain envpc5 by chlorinated solvents and alkanes. | toluene oxidation activity in pseudomonas mendocina kr1 and pseudomonas sp. strain envpc5 was induced by trichloroethylene (tce), and induction was followed by the degradation of tce. higher levels of toluene oxidation activity were achieved in the presence of a supplemental growth substrate such as glutamate, with levels of activity of up to 86% of that observed with toluene-induced cells. activity in p. mendocina kr1 was also induced by cis-1,2-dichloroethylene, perchloroethylene, chloroethane ... | 1995 | 7574658 |
| isolation of uracil auxotrophs from burkholderia cepacia atcc 25416. | two uracil auxotrophs of the phytopathogen burkolderia cepacia atcc 25416, which is known to be involved in food spoilage, were isolated by a combination of ethylmethane sulphonate and d-cycloserine counterselection. one mutant exhibited depressed orotate phosphoribosyltransferase activity while the other mutant lacked orotidine 5'-monophosphate decarboxylase activity. pyrimidine limitation of either auxotroph elevated aspartate transcarbamoylase and dihydroorotase activities by at least 1:5-fol ... | 1995 | 7576529 |
| pyrimidine synthesis in burkholderia cepacia atcc 25416. | pyrimidine synthesis in the food spoilage agent burkholderia cepacia atcc 25416 was investigated. the five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in b. cepacia atcc 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. the in vitro regulation of aspartate transcarbamoylase activity in b. cepacia atcc 25416 was studies and its activity was inhibited by pp(i), atp, gtp, ctp and utp. the enzymes cytidine deaminase, uridine phos ... | 1995 | 7576530 |
| purification and characterization of toluene 2-monooxygenase from burkholderia cepacia g4. | recent in vivo studies indicate that ring monooxygenation is a widespread mechanism by which bacteria metabolize aromatic hydrocarbons and obtain carbon and energy. in this study, toluene 2-monooxygenase from burkholderia (formerly pseudomonas) cepacia g4 was purified to homogeneity and found to be a three-component enzyme system. the reconstituted enzyme system oxidized toluene to o-cresol and o-cresol to 3-methylcatechol, an important intermediate for growth of the bacterium on toluene. steady ... | 1995 | 7578004 |
| ornibactin production and transport properties in strains of burkholderia vietnamiensis and burkholderia cepacia (formerly pseudomonas cepacia). | several strains of burkholderia vietnamiensis, isolated from the rhizosphere of rice plants, and four strains formerly known as pseudomonas cepacia including two collection strains and two clinical isolates were compared for siderophore production and iron uptake. the b. vietnamiensis (tvv strains) as well as the b. cepacia strains (atcc 25416 and atcc 17759) and the clinical isolates k132 and lmg 6999 were all found to produce ornibactins under iron starvation. the two atcc strains of b. cepaci ... | 1995 | 7580051 |
| oligonucleotide primers designed to differentiate pathogenic pseudomonads on the basis of the sequencing of genes coding for 16s-23s rrna internal transcribed spacers. | universal primers targeting conserved sequences flanking the 3' end of the 16s and the 5' end of the 23s rrna genes (rdnas) were used to amplify the 16s-23s rdna internal transcribed spacers (its) from eight species of pseudomonads which have been associated with human infections. amplicons from reference strains of pseudomonas aeruginosa, pseudomonas cepacia, pseudomonas gladioli, pseudomonas mallei, pseudomonas mendocina, pseudomonas pickettii, pseudomonas pseudomallei, and xanthomonas maltoph ... | 1995 | 7583922 |
| the emergence of a highly transmissible lineage of cbl+ pseudomonas (burkholderia) cepacia causing cf centre epidemics in north america and britain. | the rapid increase in pseudomonas (burkholderia) cepacia infection in cystic fibrosis (cf) patients suggests epidemic transmission, but the degree of transmissibility remains controversial as conflicting conclusions have been drawn from studies at different cf centres. this report provides the first dna sequence-based documentation of a divergent evolutionary lineage of p. cepacia associated with cf centre epidemics in north america (toronto) and europe (edinburgh). the involved epidemic clone e ... | 1995 | 7585148 |
| syntheses of three interglycosidic isomers of n-acetyl-beta-d-mannosaminyl-l-rhamnoses associated with o-antigens of several gram-negative opportunistic pathogens. | we achieved practical, highly stereoselective syntheses of three interglycosidic isomers of n-acetyl-beta-d-mannosaminyl-l-rhamnoses, among which a beta(1-->4)-isomer corresponds to the repeating unit of the o-antigen of lipopolysaccharide (lps) from the opportunistic pathogens pseudomonas cepacia o5 and pseudomonas aeruginosa x (meitert). the other isomers are a beta(1-->2)-disaccharide, a constituent of lps from escherichia coli o1a, and an artificial beta(1-->3)-isomer. the disaccharides were ... | 1995 | 7586068 |
| structure and mechanism of the iron-sulfur flavoprotein phthalate dioxygenase reductase. | transfer of electrons between pyridine nucleotides (obligatory two-electron carriers) and hemes or [2fe-2s] centers (obligatory one-electron carriers) is an essential step mediated by flavins in respiration, photosynthesis, and many oxygenase systems. phthalate dioxygenase reductase (pdr), a soluble iron-sulfur flavoprotein from pseudomonas cepacia, is a convenient model for the study of this type of electron transfer. pdr is folded into three domains; the nh2-terminal fmn binding and central na ... | 1995 | 7589982 |
| influence of pseudomonas aeruginosa exoproducts on virulence factor production in burkholderia cepacia: evidence of interspecies communication. | the effect of concentrated cell-free extracellular material from stationary-phase cultures of burkholderia cepacia 10661 and pseudomonas aeruginosa pao1 on virulence factor production in b. cepacia was assessed. while increasing concentrations of the b. cepacia exoproduct caused a slight increase in siderophore, lipase, and protease production in the producing organism, a significant in productivity was observed for all three virulence factors with the addition of the pao1 exoproduct. moreover, ... | 1995 | 7592496 |
| molecular cloning and sequencing of a cdna encoding alanine-glyoxylate aminotransferase 2 from rat kidney. | alanine-glyoxylate aminotransferase (agt) 2 is a pyridoxal 5'-phosphate dependent, mitochondrial enzyme which, in the rat, is expressed at a high level in the kidney. the amino acid sequences of nine tryptic and seven cnbr peptides of the rat kidney agt2 were determined. three overlapping cdnas encoding the agt2 were cloned on the basis of its partial amino acid sequences by means of a polymerase chain reaction-based approach involving rat kidney poly(a)+ rna. the complete cdna sequence comprise ... | 1995 | 7592550 |
| evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway. | the maleylacetate reductase from pseudomonas sp. strain b13 functioning in the modified ortho pathway was purified and digested with trypsin. the polypeptides separated by high-performance liquid chromatography were sequenced. alignments with the polypeptides predicted from the tfdf and tcbf genes located on plasmids pjp4 of the 2,4-dichlorophenoxyacetate-degrading alcaligenes eutrophus jmp134 and pp51 of the 1,2,4-trichlorobenzene-degrading pseudomonas sp. strain p51 as well as polypeptides pre ... | 1995 | 7601858 |
| biological activity of burkholderia (pseudomonas) cepacia lipopolysaccharide. | burkholderia cepacia has emerged as an important multiresistant pathogen in cystic fibrosis (cf), associated in 20% of colonised patients with a rapid and fatal decline in lung function. although knowledge of b. cepacia epidemiology has improved, the mechanisms involved in pathogenesis remain obscure. in this study, b. cepacia lipopolysaccharide (lps) was assessed for endotoxic potential and the capacity to induce tumour necrosis factor (tnf). lps preparations from clinical and environmental iso ... | 1995 | 7640678 |
| isolation and treatment of cystic fibrosis patients with lung infections caused by pseudomonas (burkholderia) cepacia and multiresistant pseudomonas aeruginosa. | cross-infection with pseudomonas aeruginosa and pseudomonas cepacia has been shown sometimes to occur between cystic fibrosis (cf) patients in some cf centres, in some summer camps and during some social contacts between cf patients. cohort isolation and improved hygienic precautions, however, have successfully been employed in some cf centres resulting in a decrease or elimination of cross-infection. chronic p. aeruginosa infection is in most cf patients preceded by a period of intermittent col ... | 1995 | 7643943 |
| maintenance treatment with antibiotics in cystic fibrosis patients. sense or nonsense? | the majority of patients with cystic fibrosis (cf) have chronic lung infection with a variety of organisms. it is accepted clinical practice to treat acute exacerbations of infection with appropriate oral or intravenous antibiotics. the aim of this paper is to review the use of maintenance antibiotics for these patients with chronic pulmonary infection. there is clinical trial evidence to support the use of maintenance treatment for patients chronically infected with staphylococcus aureus or pse ... | 1995 | 7643944 |
| genetic structure of a lotic population of burkolderia (pseudomonas) cepacia. | the genetic structure of a population of burkholderia (pseudomonas) cepacia isolated from a southeastern blackwater stream was investigated by using multilocus enzyme electrophoresis to examine the allelic variation in eight structural gene loci. overall, 213 isolates were collected at transect points along the stream continuum, from both the sediments along the bank and the water column. multilocus enzyme electrophoresis analysis revealed 164 distinct electrophoretic types, and the mean genetic ... | 1995 | 7646017 |
| the treatment of pulmonary infection in cystic fibrosis. | the survival of cystic fibrosis patients has improved through an aggressive, multidisciplinary approach to the therapy of pulmonary sepsis. intravenous antibiotics play a major role in the care of cystic fibrosis patients, even though it is not possible to achieve persistent bacterial eradication due to the complex microbiology and pathology of these patients. the most important pathogen in older patients is pseudomonas aeruginosa. the increasing incidence of pseudomonas cepacia, strains of whic ... | 1995 | 7652499 |
| laboratory data which differentiate meropenem and imipenem. | meropenem and imipenem are carbapenems which are distinguishable from all other currently available beta-lactam antibiotics by breadth of antibacterial spectrum and stability to beta-lactamases, but can be differentiated one from another. meropenem is relatively stable to human renal dehydropeptidase-i (dhp-i); it does not require to be co-administered with cilastatin and consequently, unlike imipenem, will be administered as a single agent. in vitro both meropenem and imipenem are active agains ... | 1995 | 7652504 |
| pediatric lung transplantation. the years 1985 to 1992 and the clinical trial of fk 506. | the application of lung transplantation to the pediatric population was a natural extension of the success realized in our adult transplantation program, which began in 1982. twenty pediatric patients (age range 3 to 18 years) have had heart-lung (n = 11), double lung (n = 8), and single lung (n = 1) transplantation procedures. the causes of end-stage lung disease were primary pulmonary hypertension (n = 7), congenital heart disease (n = 5), cystic fibrosis (n = 4), pulmonary arteriovenous malfo ... | 1993 | 7679172 |
| antagonism of pseudomonas cepacia against phytopathogenic fungi. | two strains of pseudomonas cepacia, rj3 and atcc 52796, have been identified as potential antagonists of fungal plant pathogens. we have compared the antagonistic activity of these two strains against various fungal pathogens. although both strains displayed high levels of antagonism, atcc 52796 was slightly more antagonistic than rj3. the antagonist from rj3 has been identified as the antifungal compound pyrrolnitrin after purification by hplc and characterization by uv, ir, nmr, and mass spect ... | 1993 | 7679303 |
| pseudomonas cepacia in lung transplant recipients with cystic fibrosis. | twenty-four isolated double lung transplants (ltxs) have been performed in 22 patients with cystic fibrosis, with a follow-up of 4 to 47 months. prior to ltx, all patients were colonized with pseudomonas aeruginosa, and ten patients were also colonized with pseudomonas cepacia. both organisms were specifically sought before ltx. all patients who grew p cepacia before ltx did so after ltx. five additional patients only grew this bacterium after ltx. there was no difference between those who grew ... | 1993 | 7679347 |
| purification of 3,5-dichlorocatechol 1,2-dioxygenase, a nonheme iron dioxygenase and a key enzyme in the biodegradation of a herbicide, 2,4-dichlorophenoxyacetic acid (2,4-d), from pseudomonas cepacia csv90. | an enzyme which cleaves the benzene ring of 3,5-dichlorocatechol has been purified to homogeneity from pseudomonas cepacia csv90, grown with 2,4-dichlorophenoxyacetic acid (2,4-d) as the sole carbon source. the enzyme was a nonheme ferric dioxygenase and catalyzed the intradiol cleavage of all the examined catechol derivatives, 3,5-dichlorocatechol having the highest specificity constant of 7.3 microm-1s-1 in an air-saturated buffer. no extradiol-cleaving activity was observed. thus, the enzyme ... | 1993 | 7679568 |
| iatrogenic urinary tract infection with pseudomonas cepacia after transrectal ultrasound guided needle biopsy of the prostate. | in response to an unexplained development of pseudomonas cepacia cystoprostatitis after transrectal ultrasound guided prostate biopsy, a retrospective review of records and biopsy protocol was performed at our institution. between june 5, 1990 and january 9, 1991 no documented infections occurred in 272 patients undergoing transrectal ultrasound and prostate biopsy. during the next 6 months, however, 9 of 110 patients (8.2%) presented again with infectious symptomatology after transrectal ultras ... | 1993 | 7679757 |
| optimizing fluorescent in situ hybridization with rrna-targeted oligonucleotide probes for flow cytometric identification of microorganisms. | a combination of fluorescent rrna-targeted oligonucleotide probes ("phylogenetic stains") and flow cytometry was used for a high resolution automated analysis of mixed microbial populations. fixed cells of bacteria and yeasts were hybridized in suspension with fluorescein- or tetramethylrhodamine-labeled oligonucleotide probes complementary to group-specific regions of the 16s ribosomal rna (rrna) molecules. quantifying probe-conferred cell fluorescence by flow cytometry, we could discriminate b ... | 1993 | 7679962 |
| outbreak of pseudomonas cepacia bacteremia in oncology patients. | in 1991, an outbreak of pseudomonas cepacia bacteremia (pcb) occurred among patients at an oncology clinic in alabama. a case-patient was defined as any patient at alabama oncology hematology associates (aoha) who had at least one blood culture positive for p. cepacia from 7 august through 31 october. fourteen case-patients were identified; all required hospitalization (median duration, 17 days), but none died of pcb. a cohort study assessing risk factors for pcb focused on all patients who had ... | 1993 | 7680908 |
| pseudomonas cepacia pseudobacteraemia. | | 1993 | 7681073 |
| marked phenotypic variability in pseudomonas cepacia isolated from a patient with cystic fibrosis. | characterization of the epidemiology of pseudomonas cepacia colonization in cystic fibrosis is difficult because of the phenotypic variability of isolates. a single sputum culture may yield colonies which differ in morphology, antibiotic susceptibility, and pigment production. we examined serial p. cepacia isolates from a cystic fibrosis patient which the clinical laboratory identified as separate strains; these were selected on the basis of isolation date and culture site. an attempt was made t ... | 1993 | 7681845 |
| nosocomially acquired pseudomonas cepacia infection in patients with cystic fibrosis. | | 1993 | 7683030 |
| case-control and vector studies of nosocomial acquisition of pseudomonas cepacia in adult patients with cystic fibrosis. | to examine factors associated with nosocomial acquisition of pseudomonas cepacia in adult patients with cystic fibrosis. | 1993 | 7683031 |
| degradation of diphenylether by pseudomonas cepacia et4: enzymatic release of phenol from 2,3-dihydroxydiphenylether. | 2,3-dihydroxybiphenyl dioxygenase from pseudomonas cepacia et 4 was found to catalyze the ring fission of 2,3-dihydroxydiphenylether in the course of diphenylether degradation. the enzyme was purified and characterized. it had a molecular mass of 240 kda and is dissociated by sds into eight subunits of equal mass (31 kda). the purified enzyme was found to be most active with 2,3-dihydroxybiphenyl as substrate and showed moderate activity with 2,3-dihydroxydiphenylether, catechol and some 3-subst ... | 1993 | 7683455 |
| degradation of aroclor 1221 in soil by a hybrid pseudomonad. | the hybrid pseudomonas cepacia strain jhr22 was tested for its ability to degrade aroclor 1221 in soil. the influence of supplements--mineral salts and trace elements--on the degradation was investigated. disappearance of aroclor 1221 congeners, occurrence of metabolites, and release of chloride were measured under different conditions. after 45 days the hybrid organism, strain jhr22, was still present at high numbers in soil, independently of whether the soil had been sterilized prior to inocul ... | 1993 | 7683620 |
| magnetic circular dichroism studies on the mononuclear ferrous active site of phthalate dioxygenase from pseudomonas cepacia show a change of ligation state on substrate binding. | phthalate dioxygenase from pseudomonas cepacia contains a mononuclear ferrous center that is strictly required for catalytic oxygen activation. the spectroscopic characterization of this iron site and its ligand interactions has been complicated in the past by interference from a rieske-type binuclear (2fe-2s) cluster in the enzyme, which dominates the absorption spectra and is superimposed in x-ray absorption spectra for the mononuclear site. we have used low-temperature, variable magnetic fiel ... | 1993 | 7683910 |
| dienelactone hydrolase from pseudomonas cepacia. | dienelactone hydrolases have previously been shown to play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. recently, the enzymes induced in 4-fluorobenzoate-utilizing bacteria have been classified into three groups on the basis of their specificity towards cis- and trans-dienelactone. the dienelactone hydrolase and the 3-oxoadipate enol-lactone hydrolase from pseudomonas cepacia have now been purified to apparent homogeneity and characterized with respect to ... | 1993 | 7684040 |
| pseudomonas cepacia: pulmonary infection in patients with cystic fibrosis. | this retrospective study reviews the patterns of p. cepacia pulmonary infection in 75 of a total of 872 mainly adults with cystic fibrosis, registered here during the 4 years 1987-1990; 35 (47%) were female. during this period, 55 patients acquired p. cepacia and the annual incidence and prevalence rates have remained between 1.6 and 3.1%, and 4.1 and 5.9%, respectively. the mean age at the time of the first isolation of p. cepacia was 23 years, ranging 11-45 years. sixty-eight percent of the in ... | 1993 | 7684530 |
| pseudomonas cepacia at summer camps for persons with cystic fibrosis. | pseudomonas cepacia (pc) is a multidrug-resistant, gram-negative bacillus that causes chronic colonization and infection of the respiratory tract of persons with cystic fibrosis (cf). pc colonization is usually difficult to eradicate with antimicrobial therapy and, in some patients, infection is associated with rapid decline in pulmonary function, increased hospitalization, and earlier death. previous studies have suggested person-to-person transmission of pc both within and outside of hospitals ... | 1993 | 7684813 |
| protein engineering of the 2-haloacid halidohydrolase iva from pseudomonas cepacia mba4. | the chemical modification of l-2-haloacid halidohydrolase iva (hdl iva), originally identified in pseudomonas cepacia mba4, produced as a recombinant protein in escherichia coli dh5 alpha, led to the identification of histidine and arginine as amino acid residues likely to play a part in the catalytic mechanism of the enzyme. these results, together with dna sequence and analyses [murdiyatmo, asmara, baines, bull and hardman (1992) biochem. j. 284, 87-93] provided the basis for the rational desi ... | 1993 | 7684900 |
| activation of a bacterial lipase by its chaperone. | the gene lipa of pseudomonas cepacia dsm 3959 encodes a prelipase from which a signal peptide is cleaved during secretion, producing a mature extracellular lipase. expression of lipase in several heterologous hosts depends on the presence of another gene, lima, in cis or in trans. lipase protein has been overproduced in escherichia coli in the presence and absence of the lipase modulator gene lima. therefore, lima is not required for the transcription of lipa or for the translation of the lipa m ... | 1993 | 7685908 |
| survival of pseudomonas pseudomallei strains at 5 degrees c. | the ability of 15 pseudomonas pseudomallei strains of different origin to survive at 5 degrees c was tested, in comparison with the type strains of pseudomonas aeruginosa and pseudomonas cepacia. viable cells of each strain were suspended in tryptic soy broth (diluted 1:10) at a concentration of about 10(6) cfu/ml. the suspensions were kept at 5 degrees c, and the number of viable cells was determined by counting colonies every 10 days. four strains of p. pseudomallei, including the type strain ... | 1993 | 7686201 |
| [an outbreak of pseudomonas sepsis associated with nosocomial infection in a pediatric ward]. | a five-year old girl, diagnosed with fever of unknown origin (fuo), was transferred to our hospital due to a deterioration in her general condition. pseudomonas cepacia (p. cepacia) was detected in her blood culture and she soon recovered after imipenem cilastatin (ipm/cs) administration. when the former hospital was informed of the results of her blood cultures, we learned that five other cases of fuo had occurred there within the previous two weeks in noncompromised children. except for one ca ... | 1993 | 7686202 |
| evidence for transmission of pseudomonas cepacia by social contact in cystic fibrosis. | pulmonary colonisation with pseudomonas cepacia in patients with cystic fibrosis can be associated with increased morbidity and mortality. the modes of transmission of p cepacia are, however, unclear. we used selective media and phenotypic and genomic typing systems to investigate the acquisition of p cepacia by adults with cystic fibrosis. an analysis of isolates from 210 patients attending regional clinics in edinburgh and manchester between 1986 and 1992 showed that the main cause of increase ... | 1993 | 7686239 |
| pseudomonas cepacia in cystic fibrosis: transmissibility and its implications. | | 1993 | 7686240 |
| pseudomonas cepacia in patients with cystic fibrosis attending the royal prince alfred hospital (rpah) | | 1993 | 7686291 |
| serum igg and sputum iga antibody to core lipopolysaccharide antigen from pseudomonas cepacia in patients with cystic fibrosis. | the immunological response of cystic fibrosis (cf) patients to lipopolysaccharide (lps) antigens of pseudomonas cepacia was investigated. enzyme-linked immunosorbent assays (elisa) with either p. cepacia whole cells or extracted core lps from a clinical isolate of p. cepacia as antigen were used to measure serum igg and sputum iga anti-p. cepacia antibodies. the elisa with core lps distinguished nine cf patients colonised by p. cepacia from nine age- and sex-matched non-colonised cf patients. th ... | 1993 | 7686977 |
| role of a 22-kilodalton pilin protein in binding of pseudomonas cepacia to buccal epithelial cells. | previously we have shown that many isolates of pseudomonas cepacia obtained from the sputum of patients with cystic fibrosis exhibit specific binding to purified mucins. the binding was mediated by a 22-kda protein located on peritrichous pili of the bacteria. nonpiliated bacteria did not bind to mucin. in the present study we found that both piliated and nonpiliated p. cepacia bind to buccal epithelial cells (becs) obtained from health human volunteers. scatchard plot analyses of binding data w ... | 1993 | 7687582 |
| transmission of pseudomonas cepacia by social contact in cystic fibrosis. | | 1993 | 7688061 |
| ornibactins--a new family of siderophores from pseudomonas. | novel linear hydroxamate/hydroxycarboxylate siderophores from strains of pseudomonas cepacia were isolated and named ornibactins. the ornibactins represent modified tetrapeptide siderophores, possessing the sequence l-orn1(n delta-oh, n delta-acyl)-d-threo-asp(beta-oh)-l-ser-l-orn4(n delta-oh, n delta-formyl)-1,4-diaminobutane. the n delta-acyl groups of orn1(n delta-oh, n delta-acyl) may vary and represent the three acids 3-hydroxybutanoic acid, 3-hydroxyhexanoic acid and 3-hydroxyoctanoic acid ... | 1993 | 7689374 |
| degradation of trichloroethylene by pseudomonas cepacia g4 and the constitutive mutant strain g4 5223 pr1 in aquifer microcosms. | pseudomonas cepacia g4 degrades trichloroethylene (tce) via a degradation pathway for aromatic compounds which is induced by substrates such as phenol and tryptophan. p. cepacia g4 5223 pr1 (pr1) is a tn5 insertion mutant which constitutively expresses the toluene ortho-monooxygenase responsible for tce degradation. in groundwater microcosms, phenol-induced strain g4 and noninduced strain pr1 degraded tce (20 and 50 microm) to nondetectable levels (< 0.1 microm) within 24 h at densities of 10(8) ... | 1993 | 7690223 |
| cystic fibrosis and endobronchial pseudomonas infection. | the discovery of the cystic fibrosis gene has provided new approaches to gene therapy and drug therapy. at the present time, however, we are still faced with the morbidity and mortality due to lung infections caused by pseudomonas aeruginosa and pseudomonas cepacia. the present survey of some of the recently published articles shows that applied science related to the clinical care of cystic fibrosis (cf) patients is also progressing. cross-infection in cf centers and during social contacts betw ... | 1993 | 7690637 |
| infection due to pseudomonas cepacia. | | 1993 | 7691195 |
| clinical features of pseudomonas cepacia pneumonia in an epidemic among immunocompromised patients. | between january 1990 and august 1991, there were 37 patients admitted to our department of internal medicine with hematologic malignancies or solid tumors who showed colonization of the respiratory tract with pseudomonas cepacia. extensive surveillance cultures of the environmental surfaces and respiratory equipment of the hospital revealed that all nebulizing devices were contaminated with p cepacia. to characterize this outbreak, we retrospectively reviewed the medical records of 37 patients c ... | 1993 | 7691480 |
| experimental pneumonia produced by clinical isolates of pseudomonas cepacia in mice. | an experimental pseudomonas cepacia lung infection was induced in ddy mice pretreated with cyclophosphamide. a single dose of 250 mg of cyclophosphamide per kg resulted in leukopenia which lasted for four days. at the lowest pmn levels, the mice were exposed to various doses of bacteria by either intratracheal inoculation or aerosol inhalation. experimental pneumonia was established by intratracheal inoculation of 1 x 10(7) - 2 x 10(8) colony-forming units of p. cepacia. the duration of survival ... | 1993 | 7691974 |
| antigenic analysis of pseudomonas aeruginosa and pseudomonas cepacia groel proteins and demonstration of a lipopolysaccharide-associated groel fraction in p. aeruginosa. | quantitative crossed immunoelectrophoresis was used to evaluate the antigenic similarity of pseudomonas aeruginosa and pseudomonas cepacia groel proteins. we found that the two proteins showed 75% identity. by using a panel of monoclonal antibodies against the p. aeruginosa groel protein, we identified 10 monoclonal antibodies which cross-reacted with the p. cepacia groel protein and 21 monoclonal antibodies which recognized type-specific epitopes on the p. aeruginosa groel protein. in crossed i ... | 1993 | 7692893 |
| do all pseudomonas cepacia produce carbapenemases? | | 1993 | 7693644 |
| nosocomial outbreak of pseudomonas cepacia associated with contamination of reusable electronic ventilator temperature probes. | the investigation and control of an outbreak of nosocomial pseudomonas cepacia respiratory tract colonization and infection. | 1993 | 7693800 |
| ventilator temperature sensors: an unusual source of pseudomonas cepacia in nosocomial infection. | a prospective study was undertaken to determine the source of pseudomonas cepacia colonization and infection that had affected ventilated patients in an intensive care unit (icu) for three years. thirty-eight patients undergoing mechanical ventilation were enrolled during a six-week period. samples were taken from patients, ventilator circuits and the environment for culture. p. cepacia was isolated from the condensate formed in the ventilator circuit and the source of the contamination was show ... | 1993 | 7693802 |
| source of pseudomonas cepacia: ribotyping of isolates from patients and from the environment. | ribotyping, an analysis of bacterial strain identity based on chromosomal restriction fragment length polymorphisms, was performed on 68 isolates of pseudomonas cepacia recovered from patients receiving care at the two children's hospitals in philadelphia and from environmental samples. twenty different ribotypes were identified. ribotype r3 predominated among isolates from all three sources. these findings are consistent with the acquisition of p. cepacia from the environment or by person-to-pe ... | 1993 | 7693902 |
| in vitro activity of sce-2787, a new cephalosporin with potent activity against pseudomonas aeruginosa and members of the family enterobacteriaceae. | the in vitro activity of sce-2787, 7-[(z)-2-(5-amino-1,2,4-thiadiazol-3- yl)-2-methoxyiminoacetamido]-3-(1-imidazo[1,2-b]pyridazinium)methy l-3- cephem-4-carboxylate, was compared with those of ceftazidime, ceftriaxone, and imipenem against recent clinical isolates. sce-2787 inhibited 50% of tested isolates of the family enterobacteriaceae at < or = 0.25 micrograms/ml. sce-2787 was equally active as or more active than ceftazidime and ceftriaxone against members of the enterobacteriaceae, with t ... | 1994 | 7695279 |
| role of open lung biopsy for diagnosis in lung transplant recipients: ten-year experience. | between november 1983 and august 1993, the toronto lung transplant program performed 153 transplantations in 144 recipients: 53 single-lung transplantations (slt) and 100 double-lung transplantations (dlt). thirty-eight open lung biopsies (olbs) were done in 32 (22% of all recipients): 19 in slt (36% of slt) 12 in dlt (12% of dlt), and 1 in a patient who had a slt and then a double retransplantation. six recipients underwent olb twice: 1 dlt, 3 slt, and 2 who had olb both before and after retran ... | 1995 | 7695420 |
| characterization and sequence of a novel insertion sequence, is1162, from pseudomonas fluorescens. | we have determined the nucleotide sequence of is1162, a new insertion sequence (is) isolated from pseudomonas fluorescens (pf) strain st. this is element is present in two copies on the peg plasmid harboured by pf st and in a single copy on the chromosome, adjacent to the styrene catabolic genes. is1162 is 2634 bp in length with 12-bp terminal inverted repeats (ir), and could encode four proteins (orfs), two for each strand. one strand, pro1 (62,990 da), showed a helix-turn-helix motif at the n- ... | 1995 | 7698671 |
| effect of salicylate on expression of flagella by escherichia coli and proteus, providencia, and pseudomonas spp. | osmotic stress, salicylate, and mar (multiple antibiotic resistance) mutation are known to block the expression of the ompf porin. since these conditions have also been shown to inhibit the expression of p and cfa fimbriae in escherichia coli, we speculated that they might affect the expression of flagella as well. hyperosmotic conditions have been shown to block the synthesis of flagellin and expression of flagella in e. coli (c. li, c. j. louise, w. shi, and j. adler, j. bacteriol. 175:2229-22 ... | 1995 | 7729888 |
| [comparison of mics of new quinolones obtained using agar-dilution and broth-microdilution procedures]. | minimum inhibitory concentrations (mic) of new quinolones against both gram-positive and gram-negative bacteria obtained using agar-dilution and broth-microdilution procedures were compared. a primary regression curve and a correlation coefficient (r) between 2 mics for each of ofloxacin, ciprofloxacin, tosufloxacin, sparfloxacin or balofloxacin were calculated. for the 5 quinolones, average correlation coefficients were 0.891 for the gram-positive bacteria tested, and 0.865 for the gram-negativ ... | 1995 | 7752452 |
| [nationwide survey on susceptibilities of clinical isolates to antibacterial agents in 1991]. | this study was conducted to investigate susceptibilities of clinical isolates to different antibacterial agents at 123 hospital laboratories throughout japan from september to december of 1991. in this study, identifications and susceptibility testings were carried out at each hospital laboratory. the susceptibility testing were performed using the disk dilution method recommended by nccls. staphylococcus aureus and cns showed high or moderate resistance rates to methicillin (dmppc). streptococc ... | 1995 | 7752454 |
| evolution of chlorocatechol catabolic pathways. conclusions to be drawn from comparisons of lactone hydrolases. | the aerobic bacterial degradation of chloroaromatic compounds often involves chlorosubstituted catechols as central intermediates. they are converted to 3-oxoadipate in a series of reactions similar to that for catechol catabolism and therefore designated as modified ortho-cleavage pathway. among the enzymes of this catabolic route, the chlorocatechol 1,2-dioxygenases are known to have a relaxed substrate specificity. in contrast, several chloromuconate cycloisomerases are more specific, and the ... | 1994 | 7765840 |