| [serological typing in the study of the transport of strains of pseudomonas aeruginosa in the hospital environment]. | bacteriologic investigations in an operation theatre in the surgical department of an older hospital building in bucharest detected 14 ps. aeruginosa strains (10 strains were isolated on the floor of the operation theatre, from the sterile room and wash basins, two strains from the wheels of stretchers and two from the footwear used during the transport of patients). serologic typing identified the routes of transport of the ps. aeruginosa species, making it possible to take the necessary contro ... | 1975 | 128098 |
| [detection of split products of the immunoglobulins igg, iga and igm during chronic otitis media (author's transl)]. | proteolytic enzymes such as pepsine or papaine are able to split igg antibodies into large fragments in vitro. these immunoglobulin fragments (igg, iga, igm) were now detected in vivo from the purulent secretions of cholesteatoma, chronic otitis media and radical mastoid cavities. during chronic otitis media the intact immunoglobulins are split due to the proteolytic activity of neutral proteinases. these fragments were qualitatively and quantitatively investigated by means of various immunologi ... | 1975 | 129630 |
| leucocidin from pseudomonas aeruginosa and membrane functions. | leucocidin from pseudomonas aeruginosa (strain 158) induced loss of potassium from isolated hepatocytes. the (na+-k+) stimulated atpase activity of isolated rat liver plasma membranes showed dose-dependent activation up to 56%. electron-spin-resonance (esr) measurements gave no indication of toxin-induced changes in membrane fluidity. on isolated guinea pig heart auricles the toxin produced an increase in frequency from 180/min to about 300/min, with arrhythmia and transitory flutter. on isolate ... | 1976 | 133294 |
| [hemorrhagic syndrome in newborn infants during a hospital infection with pseudomonas aeruginosa]. | in a department for neonates 21 of the 34 children contracted infections with various clinical manifestations within a relatively short interval (approximately two weeks). three of the children died and pathoanatomic examination revealed aspects characteristic of vascularitis and diffuse hemorrhage in most organs accompanied by infiltration and oedema. from the lethal cases, part of the sick and healthy children and personnel the same bacterium was isolated--pseudomonas aeruginosa, suggesting an ... | 1976 | 133447 |
| cerium nitrate: a new topical antiseptic for extensive burns. | the wounds of 60 burned patients were treated topically with cerium nitrate, which was applied either as a cream or in aqueous solution. cerium nitrate has a potent antiseptic effect in human burn wounds, especially against gram negative bacteria and fungi. pseudomonas aeruginosa was recovered from the wounds infrequently and never predominated. fungi were practically never found. no patient treated with cerium developed a necrotizing wound infection. analysis of the detailed bacteriological dat ... | 1976 | 135364 |
| the serology of pseudomonas aeruginosa analysed by means of quantitative immunoelectrophoretic methods. v. thermostability, resistance to degradation by plasmin activity and storage conditions of a polyvalent p. aeruginosa reference standard-antigen. | the stability of a polyvalent pseudomonas aeruginosa antigen (st-ag) was analysed under different experimental conditions by quantitative immunoelectrophoretic methods. st-ag did not undergo proteolytic degradation by plasmin which is present in the corresponding polyvalent rabbit antiserum (st-ab) against st-ag. storage at -30 degrees c for up to 4 years caused no qualitative changes in st-ag and, after 1 year, only minor quantitative changes could be revealed. repeated thawing and freezing cau ... | 1976 | 136862 |
| [protective effect of schizophyllan on pseudomonas aeruginosa infection of mouse (author's transl)]. | effects of schizophyllan (spg) and several antibiotics in combination against experimental pseudomonas aeruginosa infection were examined. (1) whereas the increase in protective effect of spg was not observed in combination with carbenicillin or sulbenicillin, remarkable effects could be recognized in combination with dibekacin or gentamicin (gm). the combined therapy with spg and gm proved to be most excellent. (2) the time course for changes of viable number of ps. aeruginosa in various organs ... | 1976 | 137992 |
| [transmissible resistance factor in pseudomonas aeruginosa: arguments in favor of it being a plasmid]. | the transmissible carbenicillin resistance factor r (56 be) was isolated in pseudomonas aeruginosa. it was not cured by usual agents and not isolated by ultra-centrifugation. so the plasmidic nature may be doubtful. we demonstrate in this paper that it is possible: 1) to transduce this factor into bacteria which is then able to conjugate and to transfer resistance to another bacteria, 2) to transfer carbenicillin resistance to arginine deficient strains without bringing chromosomic genes, 3) to ... | 1976 | 139973 |
| [isolation of a pseudomonas aeruginosa strain with a r plasmide from the case of nosocomial infection (author's transl)]. | | 1977 | 140013 |
| differential chemical protection of mammalian cells from the exotoxins of corynebacterium diphtheriae and pseudomonas aeruginosa. | many drugs or chemicals had markedly different effects on the cytotoxicity induced by pseudomonas aeruginosa exotoxin a (pe) or corynebacterium diphtheriae exotoxin (de). the glycolytic inhibitor naf protected cells from de but potentiated the cytotoxicity of pe. another energy inhibitor, salicylic acid, also protected cells from de but had no effect with pe. colchicine and colcemid did not affect the cytotoxicity of either toxin. cytochalasin b exhibited a modest protection from de but no effec ... | 1977 | 141424 |
| effects of membrane-energy mutations and cations on streptomycin and gentamicin accumulation by bacteria: a model for entry of streptomycin and gentamicin in susceptible and resistant bacteria. | several mutants of escherichia coli affecting aerobic energy generation and energization of the bacterial membrane have been examined for their effect on streptomycin and gentamicin accumulation and susceptibility. a heme-deficient mutant (k207) and two mutants (cj-8 [colicin k insensitive] and nr-70) associated with defective aerobic active transport were associated with decreased transport of streptomycin and gentamicin and increased resistance to those antibiotics. these mutants also exhibite ... | 1977 | 143238 |
| [pathogenicity expressed by inhibition of the pasteur effect]. | the experiments carried out demonstrate that under the influence of tetanus exotoxin, gram-negative bacteria endotoxins, staphylococcal infection and infestation with tr. spiralis, inhibition of the pasteur effect occurs. recently published data show that the same manifestation of pathogenicity is induced by diphtheria alpha and delta exotoxin, staphylococcal toxin, pseudomonas aeruginosa exotoxin, staphylococcal enterotoxin, streptolysin o, infections with cl. perfringens, pasteurella and ricke ... | 1977 | 143708 |
| in vitro activity of spectinomycin against recent urinary tract isolates. | the susceptibilities to spectinomycin of 303 recent urinary tract isolates were determined and compared to the susceptibilities of those strains to ampicillin, tetracycline, and gentamicin. based on minimal inhibitory concentrations, 84% of escherichia coli, klebsiella, and enterobacter, 31% of other enterobacteriaceae, 7% of staphylococcus aureus and streptococcus (including enterococci), and 0% of pseudomonas aeruginosa were susceptible to concentrations of spectinomycin that are easily surpas ... | 1977 | 144457 |
| [the effect of zinc and cobalt ions on the chromogenesis of pseudomonas aeruginosa in the presence of methylphosphonic acid]. | adding zinc or cobalt sulphate into synthetic culture media of pseudomonas aeruginosa was followed by partial or total inhibition of pyocyanine production, varying with the nature of the chemical source of phosphorus. an antagonism between zn2+ and co2+ ions was shown. | 1977 | 145276 |
| synergistic effects of chlorpromazine and perphenazine on several chemotherapeutic agents. i. general profile of the effects measured by the filter paper strip-agar diffusion method with escherichia coli and pseudomonas aeruginosa. | the combination effects of chlorpromazine (cpz) and periphenazine (ppz) with beta-lactam antibiotics (ampicillin, carbenicillin, cefazolin) and nalidixic acid group compounds (nalidixic acid, piromidic acid and pipemidic acid) have been estimated to be synergistic by the filter paper strip-agar diffusion method (dye's method) with escherichia coli and pseudomonas aeruginosa as test organisms. the observed synergism might be associated with their inhibition of various enzymes including atpase and ... | 1978 | 145936 |
| [microbiological studies on mouth-ringing, cooling and turbine-spray waters]. | microbiological examinations of mouth-cleaning, cooling, and turbine spray waters from various types of dental units showed different degrees of water contamination. the detection of pseudomonas aeruginosa, because of the classification of this organism as a "problem germ", indicates the necessity for a more critical analysis of water used in stomatology including the elimination of possible sources of contamination. | 1977 | 146368 |
| mannitol and fructose catabolic pathways of pseudomonas aeruginosa carbohydrate-negative mutants and pleiotropic effects of certain enzyme deficiencies. | mutant strains of pseudomonas aeruginosa pao were isolated on the basis of their inability to utilize mannitol as sole carbon source for growth. four linkage groups (i through iv) among these mutant strains were resolved by two-factor crosses using the general transducing phage f116, and the strains appeared to contain point mutations as evidenced by ability to give rise to spontaneous revertants with wild phenotype on mannitol minimal agar. group i strains were affected only in ability to grow ... | 1978 | 146701 |
| [effect of haemolysin of pseudomonas aeruginosa on ehrlich ascites tumor cells]. | haemolysin of pseudomonas aeruginosa exerts toxic effects upon the metabolism of ehrlich ascitic tumor cells : morphological changes appear readily ; respiration is inhibited more slowly ; the lethal effect determined by intraperitoneal injection of tumor cells is neutralized. inhibition with human normal sera is complete for the hemolytic action, but incomplete for the cytopathic action. | 1978 | 157200 |
| [cytotoxic action of pseudomonas aeruginosa hemolysin]. | the cytopathic action of haemolysin of pseudomonas aeruginosa has been studied in mouse and human leucocytes. the morphological changes suggest that haemolysin affects the molecular architecture of the cell membrane, whose permeability is increased. it does not induce non-specific stimulation of peripheral lymphocytes. normal sera and albumin neutralize the hemolytic activity of haemolysin; this inhibition is also observed, to a les extent, on the lytic action on leucocytes. this raises the poss ... | 1979 | 158418 |
| pseudomonas aeruginosa infection depresses contact sensitivity to oxazolone by enhancing suppressor cell activity. | the depression of contact sensitivity to oxazolone in mice infected with pseudomonas aeruginosa was studied. in oxazolone-sensitized mice, p. aeruginosa infection affects cell proliferation in the lymph nodes draining the site of sensitization. this impaired cell proliferation does not seem to be due to an altered lymphocyte reactivity, since lymph node and spleen cells from infected animals show a normal mitotic responsiveness to both t and b cell mitogens. in addition, the draining lymph nodes ... | 1979 | 158702 |
| depression of contact sensitivity by pseudomonas aeruginosa-induced suppressor cells which affect the induction phase of immune response. | the cellular basis of depression of contact sensitivity to oxazolone in mice injected with pseudomonas aeruginosa was studied. cells from draining lymph nodes of mice sensitized with oxazolone 18 h previously were able to induce contact sensitivity to normal mice when administered in their footpads. in contrast, cells from draining lymph nodes of p. aeruginosa-injected and oxazolone-sensitized donors failed to induce contact sensitivity when injected in the footpad of normal mice and were capabl ... | 1979 | 159263 |
| deoxyribonucleic acid sequence homologies among bacterial insertion sequence elements and genomes of various organisms. | plasmid and phage deoxyribonucleic acid (dna) harboring bacterial insertion sequence (is) elements is1, is2, and is5 were characterized and used as probes to detect homologous sequences in various procaryotic and eucaryotic genomes. the hybridization method used permits the detection of sequences partially homologous to the elements. hybridization of the is-containing probes to each other revealed a region of limited homology between is1 and is2. homologous sequences were then detected by comput ... | 1979 | 159291 |
| pseudomonas aeruginosa gram-negative folliculitis. | three patients with sudden, unmanageable exacerbation of acne vulgaris were shown to have gram-negative folliculitis due to pseudomonas aeruginosa. in each patient, the source of the pseudomonas proved to be an otitis externa infection. in contrast to previous cases of gram-negative folliculitis due to proteus, escherichia coli, or klebsiella, the anterior nares were not colonized. treatment of the otitis externa and the gram-negative folliculitis with acetic acid compresses and topical antibiot ... | 1979 | 159666 |
| [typization of pseudomonas aeruginosa by means of pyocins (pyocinogenotypie) (author's transl)]. | | 1979 | 161515 |
| [second possibility of employing typization of pseudomonas aeruginosa by means of pyocins (pyocinotypia) (author's transl)]. | | 1979 | 161865 |
| identification of nonfermentative gram-negative bacteria in the clinical laboratory. | a simplified, concise scheme was developed for the identification of nonfermentative, gram-negative bacteria which have most frequently been reported in the literature as definite or possible agents of human disease. these organisms included apyocyanogenic pseudomonas aeruginosa, p. fluorescens, p. putida, p. stutzeri, p. maltophilia, p. putrefaciens, p. cepacia, p. alcaligenes, flavobacterium species, bordetella bronchiseptica, acinetobacter anitratum (herellea vaginicola), a. iwoffi (mima poly ... | 1975 | 163060 |
| 6-phosphogluconate dehydratase deficiency in pleiotropic carbohydrate-negative mutant strains of pseudomonas aeruginosa. | mutants of pseudomonas aeruginosa, strain pao, have been isolated that are unable to grow on mannitol, glucose, gluconate, or 2-ketogluconate, cut that exhibit wild-type growth on pyruvate, lactate, citrate, succinate, or acetate. although some of these mutants were also unable to grow on glycerol, the mutations formed a single linkage group by quantitative transductional analysis with phage f116 on glucose minimal agar medium. cell extracts of all mutant strains were either lacking or severely ... | 1975 | 163817 |
| prevention of gram-negative bacillary pneumonia using polymyxin aerosol as prophylaxis. ii. effect on the incidence of pneumonia in seriously ill patients. | all 744 patients admitted to a respiratory-surgical intensive care unit (rsicu) were included in a prospective study of the effects of a polymyxin (2.5 mg/kg body wt/day in six divided doses) or a placebo aerosol sprayed into the posterior pharynx and tracheal tube (if present), during 11 alternating 2-mo treatment cycles. the incidence of upper airway colonization in the rsicu with pseudomonas aeruginosa was 1.6% during the polymyxin treatment cycles (total 374 patients) and 9.7% during the pla ... | 1975 | 163848 |
| hydrogen cyanide, a secondary metabolite of pseudomonas aeruginosa. | seventy-four of 110 strains of pseudomonas aeruginosa tested produced detectable amounts of hcn from growth in 2% peptone or nutrient agar. of the 25 species of12 bacterial and fungal genera tested, other than p. aeruginosa, only p. fluorescens and p. polycolor gave positive hcn tests. cyanide is produced after cessation of active growth. iron was stimulatory to cyanogenesis in concentration above 1 mum, while copper, zinc, cobalt, and manganese at concentrations of 20 mum had no effect. cyanoge ... | 1975 | 164997 |
| study of pseudomonas haemolysin. ii. characteristics of pseudomonas haemolysin. | haemolysins of 4 strains of pseudomonas aeruginosa producing different fractions [ii, 2 + ii, i + ii + iii] were studied. the production of specific antibodies was demonstrated making possible the immunoelectrophoretic record of the whole lysin and of the individual fractions. tests on laboratory animals [mice, rabbits] showed a high degree lethal and dermonecrotic property of the lysin containing the fractions ii + iii and i + ii + iii. | 1975 | 165235 |
| [comparative studies of the therapeutic effect of colistin methanesulfonate administered intramuscularly and intravenously on the acute bacterial infection in mice (author's transl)]. | the therapeutic potencies of colistin methanesulfonate (clm) was assessed quantitatively in acute infection of mice with clinically isolated strains of escherichia coli and pseudomonas aeruginosa, and effect of different routes of administration was compared. there was no detectable difference in the therapeutic effect of clm when intramuscular (im) or intravenous (iv) administration was initiated one hour after the infection. on the other hand, a significant difference in ed50 given by im and i ... | 1975 | 165326 |
| genetic analysis of radiation sensitive and chemical-mutagen sensitive mutants of pseudomonas aeruginosa. | thirty-five mutants of pseudomonas aeruginosa sensitive to methyl methanesulfonate (mms) have been genetically characterized. they constitute ten separable groups as defined by transduction and conjugation. three of the groups have been shown to be cotransducible with auxotrophic markers. | 1975 | 165402 |
| chemotherapeutic nitroheterocycles. 18. 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups. | 2-(5-nitro-2-imidazolylmethylene)-1-indanones, -1-tetralones, and -acetophenones substituted by aminoalkoxy groups and related compounds (41-69, table ii) were synthesized and their antimicrobial activities were evaluated (table iii). some of these compounds (e.g. 47, 52, and 59) suprisingly exhibited a broad antibacterial spectrum including proteus species and pseudomonas aeruginosa. extraordinary antitrichomonal activities could also be observed in vitro (mic of compound 59, 0.0004 pg/ml) and ... | 1975 | 166185 |
| nad-dependent inhibition of protein synthesis by pseudomonas aeruginosa toxin,. | pseudomonas aeruginosa toxin (pa toxin) inhibits protein synthesis in a reticulocyte cell-free system. the inhibition requires nad and results in a block at an elongation step of polypeptide assembly. pa toxin was found to act like diphtheria toxin fragment a. both toxins catalyze the transfer of radioactivity from nicotinamide(u-14-c)adenine dinucleotide ((14-c)nad) into covalent linkage with the 100,000 dalton elongation (ef-2) protein. furthermore, in the presence of a limiting amount of ef-2 ... | 1975 | 166383 |
| aerosol polymyxin and pneumonia in seriously ill patients. | pneumonia caused by pseudomonas aeruginosa occurs frequently in critically ill patients and is associated with a mortality rate of 70 per cent. an aerosol of polymyxin b was administered (2.5 mg per kilogram per day) to the upper airways of 292 patients in a respiratory-surgical intensive-care unit during a seven-month period, in an attempt to prevent ps. aeruginosa pneumonia. although only one of the patients studied acquired pneumonia due to ps. aeruginosa, 10 others acquired pneumonia caused ... | 1975 | 168487 |
| electron transfer between azurin and cytochrone c-551 from pseudomonas aeruginosa. | the electron-transfer reaction between azurin and cytochrome c1 isolated from pseudomonas aeruginosa was investigated by rapid-reaction techniques. temperture-jump studies clearly reveal two chemical relaxations, the amplitudes of which have ikentical spectral distributions, but relaxation times show different dependencies on reactant concentrations. stopped experiments also showed complex kinetics. a model is proposed which is consistent with the kinetic and equilibrium data obtained. the centr ... | 1975 | 168867 |
| the effect of temperature on the cytochrome pattern and respiration of pseudomonas aeruginosa. | | 1975 | 169162 |
| rabbit corneal damage produced by pseudomonas aeruginosa infection. | gross, light microscopic, and electron microscopic examination of the rabbit corneal destruction produced by experimental pseudomonas aeruginosa infections revealed a combination of acute inflammation and liquefaction necrosis of the cornea. degeneration of the epithelial cells and the start of polymorphonuclear leukocyte infiltration of the cornea occurred initially. these changes were followed by loss of the epithelium, degeneration and loss of the keratocytes and endothelium, loss of the char ... | 1975 | 169202 |
| [sensitivity of bacillus pyocyaneus (pseudomonas aeruginosa) to antiseptics and antibiotics. viii. action of the combination zinc bacitracin, neomycin sulfate and polymyxin b sulfate on 155 strains of pseudomonas aeruginosa (1st part)]. | | 1975 | 169602 |
| activity of gentamicin, tobramycin, polymyxin b, and colistimethate in mouse protection tests with pseudomonas aeruginosa. | mouse protection tests were carried out with four antibiotics and six strains of pseudomonas aeruginosa. all strains were susceptible to all four antibiotics by an in vitro test. a heavier bacterial inoculum increased the mean effective dose of gentamicin and tobramycin, but not polymyxin b. second and third doses of gentamicin in the mouse protection test made little change in the mean effective dose. in the mouse protection tests, tobramycin was the most active antibiotic if the results were a ... | 1975 | 169729 |
| n-acetylglutamate 5-phosphotransferase of pseudomonas aeruginosa. purification and ligand-directed association-dissociation. | n-acetylglutamate 5-phosphotransferase (atp: n-acetyl-l-glutamate 5-phosphotransferase ec 2.7.2.8), the second enzyme of arginine biosynthesis, was purified over 2000-fold from pseudomonas aeruginosa. the purification procedure involved a heat treatment, ammonium sulfate precipitation, and chromatography on deae-cellulose, sephadex g-150, and hydroxyapatite. the purified enzyme was greater than 90% pure as judged by analytical polyacrylamide gel electrophoresis. a molecular weight of approximate ... | 1975 | 170089 |
| catabolite repression of pseudomonas aeruginosa amidase: the effect of carbon source on amidase synthesis. | synthesis of the pseudomonas aeruginosa aliphatic amidase was repressed severely by succinate and malate and less severely by glucose, acetate or lactate. amidase synthesis in inducible and constitutive strains was stimulated by cyclic amp, which also gave partial relief to catabolite repression produced by the addition of lactate to cultures growing in pyruvate medium. mutants which were resistant to catabolite repression were isolated from succinate+lactamide medium. | 1975 | 170365 |
| catabolite repression of pseudomonas aeruginosa amidase: isolation of promotor mutants. | among mutants of pseudomonas aeruginosa isolated from fluoroacetamide medium were some which synthesized amidase at about 5% of the rate of the parent constitutive strain, pac101. seven fluoroacetamide-resistant mutants with low amidase activity gave rise to secondary mutant strains on succinate+butyramide plates. one appeared to be an 'up-promotor' mutant and synthesized amidase at a high rate. this mutant, pac433, was not stimulated by cyclic-amp and was much less sensitive to catabolite repre ... | 1975 | 170366 |
| degradation of phospholipid in pseudomonas aeruginosa induced by polymyxin b. | effects of polymyxin b on the synthesis and degradation of lipid, ribonucleic acid (rna) and protein in pseudomonas aeruginosa were investigated. it was found that polymyxin b caused a marked degradation of the lipid fraction which was prelabeled with (3h-2)-glycerol. thin-layer chromatographic analysis indicated that the main degraded lipids were phosphatidylethanolamine and phosphatidylglycerol, which constituted 80% and 15% of the total phospholipids of this organism, respectively. polymyxin ... | 1975 | 171247 |
| pseudomonas cytochrome c peroxidase xi. kinetics of the peroxidatic oxidation of pseudomonas respiratory chain components. | the steady state kinetics of cytochrome c peroxidase from pseudomonas aeruginosa (paccp) has been studied by initial velocity techniques using several cytochromes c (550 and 555 p. aeruginosa; 551 p. fluorescens) and pseudomonas azurin as electron donors and hydrogen peroxide as electron acceptor. from the initial velocity patterns a sequential mechanism with compulsory substrate-binding order is proposed for paccp. a comparative kinetic study of the peroxidatic oxidation of cytochrome c-551 (p. ... | 1975 | 171896 |
| proceedings: repression of cholinesterase production in pseudomonas aeruginosa by nh4+ and glucose and its prevention by cyclic amp. | | 1975 | 173681 |
| the influence of nutrient limitation in a chemostat on the sensitivity of pseudomonas aeruginosa to polymyxin and to edta. | | 1975 | 173702 |
| ultrastructural study of polymyxin-resistant isolates of pseudomonas aeruginosa. | upon exposure to 6,000 u of polymyxin b sulfate per ml, cells of the polymyxin-sensitive pao 1 strain of pseudomonas aeruginosa displayed in thin sections long projections arising from the outer membrane of the cell wall and extensive cytoplasmic degradation with accumulation of cytoplasmic membrane infoldings. polymyxin-resistant isolates derived from the pao 1 strain, however, grew well in the presence of 6,000 u of polymyxin per ml and exhibited none of these effects, having instead the appea ... | 1976 | 173706 |
| combinations of antimicrobial agents. i. the in vitro sensitivity of 100 strains of pseudomonas aeruginosa to polymyxin b, colistin, carbenicillin, gentamicin and doxycycline and to various combinations of these antibiotics. | the mics for 100 strains of pseudomonas aeruginosa were determined for polymyxin b, colistin, carbenicillin, gentamicin and doxycycline and various combinations of these antibiotics. this study was carried out by means of a tube dilution method. additive actions were observed in the combinations of polymyxin b plus carbenicillin, polymyxin b plus gentamicin, polymyxin b plus doxycycline, carbenicillin plus doxycycline and gentamicin plus doxycycline. a synergistic action was observed for the com ... | 1975 | 174496 |
| a temperature-jump study of the reaction between azurin and cytochrome c oxidase from pseudomonas aeruginosa. | the electron-transfer reaction between azurin and the cytochrome oxidase from pseudomonas aeruginosa was investigated by temperature-jump relaxation in the absence of o2 and in the presence of co. the results show that: (i) reduced azurin exists in two forms in equilibrium, only one of which is capable of exchanging electrons with the pseudomonas cytochrome oxidase, in agreement with m. t. wilson, c. greenwood, m. brunori & e. antonini (1975) (biochem. j. 145, 449-457); (ii) the electron transfe ... | 1975 | 174553 |
| the reaction of pseudomonas aeruginosa cytochrome c oxidase with carbon monoxide. | the binding of co to ascorbate-reduced pseudomonas cytochrome oxidase was investigated by static-titration, stopped-flow and flash-photolytic techniques. static-titration data indicated that the binding process was non-stoicheiometric, with a hill number of 1.44. stopped-flow kinetics obtained on the binding of co to reduced pseudomonas cytochrome oxidase were biphasic in form; the faster rate exhibited a linear dependence on co concentration with a second-order rate constant of 2 x 10(4) m-1-s- ... | 1975 | 174556 |
| conformational equilibria accompanying the electron transfer between cytochrome c (p551) and azurin from pseudomonas aeruginosa. | the redox reaction between cytochrome c (cyt c) (p-551) and the blue copper protein azurin, both from pseudomonas aeruginosa, was studied using the temperature-jump technique. two relaxation times were observed in a mechanism assumed to involve three equilibria. the fast relaxation time (0.4 less than tau less than 8 ms) was ascribed to the electron exchange step. the slow relaxation time (tau congruent to 37 ms) was assigned to a conformational equilibrium of the reduced azurin that was coupled ... | 1976 | 174718 |
| the role of glucose limitation in the regulation of the transport of glucose, gluconate and 2-oxogluconate, and of glucose metabolism in pseudomonas aeruginosa. | the pathway of glucose metabolism in pseudomonas aeruginosa was regulated by the availability of glucose and related compounds. on changing from an ammonium limitation to a glucose limitation, the organism responded by adjusting its metabolism substantially from the extracellular direct oxidative pathway to the intracellular phosphorylative route. this change was achieved by repression of the transport systems for gluconate and 2-oxogluconate and of the associated enzymes for 2-oxogluconate meta ... | 1976 | 176310 |
| [reversible bactericidal defect of leukocytes. pseudomonas meningitis in a child with diabetes mellitus (author's transl)]. | a 3-year-old boy with diabetes mellitus contracted meningitis caused by pseudomonas aeruginosa. the disease lasted for several months in spite of antibiotic treatment. diabetes mellitus was poorly controlled and hypoglycemia often occured. immunoglobulin levels were normal. investigations of leukocyte functions showed (no bactericidal activity), decreased phagocytic activity and very low nbt dye reduction by neutrophils during phagocytosis. under the administration of polymixin, leukocyte transf ... | 1976 | 176580 |
| [autoradiographic study of a bacterial colony. application to the effects of polymyxin on pseudomonas aeruginosa colonies]. | the autoradiograph of a colony of ps. a. which has been transferred, during growth, on a medium added with polymyxin and tritiate leucin makes it possible to locate an upper zone with a high metabolic activity and a basal zone with no metabolic activity. the latter, which consists of lysed cells, acts probably as a selective filter against the drug. | 1975 | 177223 |
| effect of antibiotics on the phagocytosis and killing of pseudomonas aeruginosa by rabbit polymorphonuclear leukocytes. | the effect of antibiotics on phagocytosis and killing of pseudomonas aeruginosa by rabbit polymorphonuclear leukocytes was studied. carbenicillin and sulbenicillin, when added to an incubation medium at a concentration as low as 1/16 mic, increased phagocytosis and killing of p. aeruginosa by pmn. meanwhile, gentamicin and 3',4'-dideoxykanamycin b gave no influence on the pmn activity, and polymyxin b and colistin enhanced the activity only at mic. the pmn activity was not facilitated even when ... | 1976 | 178484 |
| temperature-dependent inactivating factor of pseudomonas aeruginosa exotoxin a. | the adenosine diphosphate ribosyl transferase activity of pseudomonas aeruginosa exotoxin a(pa toxin) was found to be rapidly destroyed by heating at 45 to 60c but not by heating at 70 to 90c (for at least 30 min). this phenomenon has been previously described for other bacterial toxins (staphylococcal alpha-toxin and vibrio parahaemolyticus hemolysin) and is termed an arrhenius effect. in contrast, the arrhenius effect was not seen when the pa toxin was heat-treated as above and tested for cell ... | 1976 | 178606 |
| change in the ratio of cytochrome oxidase activity to nitrite reductase activity of pseudomonas aeruginosa nitrite reductase with the kind of c-type cytochrome used as an electron donor. | the ratio between the nitrite reductase and cytochrome oxidase activities of pseudomonas aeruginosa nitrite reductase [ec 1.9.3.2.] varies with kind of c-type cytochrome used as the electron donor. withe cytochrome c-548, 554 (micrococcus sp.), the nitrite reductase activity is greater than the cytochrome oxidase activity, while the former is smaller than the latter with cytochrome c-554 (navicula pelliculosa). the aerobic oxidation catalyzed by this enzyme of denitrifying bacterial ferrocytoch ... | 1976 | 178646 |
| isolation of pseudomonas aeruginosa and other bacterial species from ornamental aquarium plants. | ornamental aquarium plants were demonstrated to carry as many as 10(8) viable mesophilic bacteria per g. gram-negative organisms predominated among the 19 genera of bacteria identified. pseudomonas aeruginosa was readily isolated from 53% of the samples tested. | 1976 | 180888 |
| cytochrome oxidase from pseudomonas aeruginosa. iv. reaction with oxygen and carbon monoxide. | the reaction between a cytochrome oxidase from pseudomonas aeruginosa and oxygen has been studied by a rapid mixing technique. the data indicate that the heme d1 moiety of the ascorbate-reduced enzyme is oxidized faster than the heme c component. the oxidation of heme d1 is accurately second order with respect to oxygen and has a rate constant of 5.7 - 10(4) m-1 - s-1 at 20 degrees c. the oxidation of the heme c has a first order rate constant of about 8 s-1 at infinite concentration of o2. the ... | 1976 | 181054 |
| in vivo activation by polymyxin b of phospholipase from pseudomonas aeruginosa and escherichia coli. | | 1976 | 181357 |
| resistance of pseudomonas aeruginosa to antimicrobial drugs. | | 1975 | 181789 |
| a purification procedure for the soluble cytochrome oxidase and some other respiratory proteins from pseudomonas aeruginosa. | the production of the soluble cytochrome oxidase/nitrite reductase in the bacterium pseudomonas aeruginosa is favoured by anaerobic conditions and the presence of kno3(20g/l) in the culture medium. of three methods commonly used for the disruption of bacterial suspensions (ultrasonication, liquid-shear homogenization and glass-bead grinding), sonication proved the most efficient in releasing the pseudomonas cytochrome oxidase. a polarographic assay of pseudomonas cytochrome oxidase activity with ... | 1976 | 183750 |
| [a case of fulminant primary pseudomonas aeruginosa pneumonia with bacteremia in a healthy man. discussion of the specificity of the microscopic lesions]. | | 1976 | 184545 |
| therapeutic effects of heparin on pseudomonas-induced corneal ulceration. | fifty organisms of pseudomonas aeruginosa were injected intralamellarly in both eyes of 60 albino rabbits. twenty-four hours later, all eyes were graded and stratified into six groups of ten rabbits with equivalent infections. treatment was begun with gentamicin alone or 0.3% polymyxin b alone, and in combination with acetylcysteine, 0.3m edetate disodium, and 2.500 units/ml of heparin sodium, four times daily. we also compared topical colistin and colistin and topical heparin. after two weeks, ... | 1976 | 184711 |
| the release and characterization of some periplasm-located enzymes of pseudomona aeruginosa. | pseudomonas aeruginosa (atcc 9027) releases four periplasm-located enzymes, i.e., ribonuclease (ec 3.1.4.22; ec 3.1.4.23), alkaline phosphatase (ec 3.1.3.1), cyclic-2', 3'-phosphodiesterase (ec 3.1.4.d), and 5'-nucleotidase (ec 3.1.3.5) into the medium during growth. ribonuclease and alkaline phosphatase are classed as enzymes which are readily extracted by osmotic shock and spheroplast formation whereas cyclic-2',3'-phosphodiesterase and 5'-nucleotidase are classed as enzymes which are not read ... | 1976 | 184895 |
| inter- and intralaboratory variability in antibiotic susceptibility tests with pseudomonas aeruginosa and enterobacteriaceae. | a two-phase collaboratice study was designed for definition of the extent to which results of disk diffusion tests can be reproduced. two currently recommended techniques, the kirby-bauer method and the agar overlay method, were used. of special concern were tests of pseudomonas aeruginosa with moderate susceptibility to carbenicillin; gentamicin, tobramycin, polymyxin b, and colistin were also tested with p. aeruginosa as well as with klebsiella pneumoniae, escherichia coli, and proteus species ... | 1976 | 185302 |
| synergistic effect on mortality in mice with murine cytomegalovirus and pseudomonas aeruginosa, staphylococcus aureus, or candida albicans infections. | a synergistic effect on mortality was demonstrated in a combined infection of mice with murine cytomegalovirus (mcmv) and pseudomonas aeruginosa, staphylococcus aureus, or candida albicans. mice infected intraperitoneally with a 0 to 20% lethal dose inoculum of mcmv 3 days prior to the intravenous injection of a 0 to 20% lethal dose inoculum of either the bacteria or fungus demonstrated a striking enhancement of mortality. mcmv-infected mice given pseudomonas or staphylococcus exhibited a 90 to ... | 1976 | 186411 |
| [infections transmitted in swimming pools]. | public swimmingpools can be the source of infections due to micro-organism such as mycobacterium balnei, adeno and enteroviruses, the virus of plantar warts and molluscum contagiosum, the tric-agent of swimmingpool-conjonctivitis and pathogenic fungi. the transmission of trichomonas vaginalis is considered unlikely-water of pools, supposed to present satisfactory qualities by standard controls, was found to contain pathogenic staphylococci and pseudomonas aeruginosa. effective preventive measure ... | 1976 | 186978 |
| cyclic adenosine 3',5'-monophosphate levels and activities of adenylate cyclase and cyclic adenosine 3',5'-monophosphate phosphodiesterase in pseudomonas and bacteroides. | a modified gilman assay was used to determine the concentrations of cyclic adenosine 3',5'-monophosphate (camp) in rapidly filtered cells and in the culture filtrates of pseudomonas aeruginosa, escherichia coli k-12, and bacteroides fragilis. in p. aeruginosa cultures, levels of camp in the filtrate increased with the culture absorbance (3.5 to 19.8 x 10(-9) m) but did not vary significantly with the carbon source used to support growth. intracellular concentrations (0.8 to 3.2 x 10(-5) m) were ... | 1977 | 187575 |
| comparative studies on isolated rat hepatocytes and as-30d hepatoma cells with leucocidin from pseudomonas aeruginosa. | leucocidin, one of the cytotoxic principles of pseudomonas aeruginosa induces potassium loss and swelling in isolated hepatocytes and in as-30d ascites hepatoma cells in a dose and time dependent manner. hepatoma cells are more sensitive than normal hepatocytes. as shown by scanning electron microscopy the volume increase of both types of cells is accompanied by disappearance of microvilli. in contrast to phalloidin poisoning no protrusions were formed when the cells were exposed to leucocidin u ... | 1976 | 187964 |
| mössbauer and epr spectroscopy of protocatechuate 3,4-dioxygenase from pseudomonas aeruginosa. | protocatechuate 3,4-dioxygenase (ec 1.13.11.3) from pseudomonas aeruginosa has been investigated by epr and mössbauer spectroscopy. low temperature mössbauer data on the native enzyme (fe3+, s = 5/2) yields a hyperfine field hsat=-525 kg at the nucleus. this observation is inconsistent with earlier suggestions, based on epr data of a rubredoxin-like ligand environment around the iron, i.e. a tetrahedral sulfur coordination. likewise, the dithionite-reduced enzyme has mössbauer parameters unlike ... | 1976 | 188463 |
| mechanism of action of pseudomonas aeruginosa exotoxin aiadenosine diphosphate-ribosylation of mammalian elongation factor 2 in vitro and in vivo. | previous studies showed that pseudomonas aeruginosa exotoxin a (pa toxin) catalyzes nicotinamide adenine dinucleotide (nad)-dependent inhibition of protein synthesis in a rabbit reticulocyte lysate and transfer of radioactivity from [14c]adenine-labeled nad to a protein having the same molecular weight as elongation factor 2 (ef-2) (b.h. iglewski and d. kabat, 1975). such an inhibited protein-synthesizing lysate was restored to activity by addition of a protein from normal mouse liver which co-p ... | 1977 | 188760 |
| frentizole, a novel immunosuppressive, and azathioprine: their comparative effects on host resistance to pseudomonas aeruginosa, candida albicans, herpes simplex virus, and influenza (ann arbor) virus. | frentizole, 1-(6-methoxy-2-benzothiazolyl)-3-phenyl urea, a new immunosuppressive agent, and azathioprine were administered subcutaneously at predetermined immunosuppressive dose levels of azathioprine and up to 50 times an immunosuppressive dose level of frentizole. after 10 days of treatment at these dose levels, the experimental groups were inoculated intraperitoneally with pseudomonas aeruginosa or herpes simplex virus, inoculated intraveneously with candida albicans, or infected by aerosol ... | 1977 | 188761 |
| the corneal response to pseudomonas aeruginosa: histopathological and enzymatic characterization. | the host response to the intrastromal injection of heat-inactivated pseudomonas aeruginosa cells was studied. an extensive polymorphonuclear leukocyte (pmn) infiltration, which progressed to ulceration within 1 week, was observed. in some cases descemetoceles also developed. only a limited degree of pmn infiltration and no ulcerations were observed at 1 week in eyes treated with corticosteroids. collagenase and proteolytic enzymes capable of degrading proteoglycans were found in the ulcerated co ... | 1977 | 188778 |
| plasmid modification of radiation and chemical-mutagen sensitivity in pseudomonas aeruginosa. | the r factor pmg2 protects pseudomonas aeruginosa against the lethal effects of ultraviolet (u.v.) and gamma irradiation, and methyl methanesulphonate and n-methyl-n'-nitro-n-nitrosoguanidine treatment. enhanced survival occurs in strains of uvr+ rec+ (wild-type) genotype and a variety of uvr rec+ type mutants. no protection occurs in a rec a-type mutant. the plasmid also enhances u.v.-induced mutagenesis. these effects appear to be due to host-cell controlled plasmid-determined dna repair funct ... | 1977 | 188973 |
| comparative effect of methioninyl adenylate on the growth of salmonella typhimurium and pseudomonas aeruginosa. | the bacteriostatic effect of methioninyl adenylate(mamp)--a specific inhibitor of the enzyme methionyl-trna synthetase--was investigated on salmonella typhimurium and pseudomonas aeruginosa. 0.1 mm of this molecule added to the culture, inhibits the growth of s. typhimurium. the inhibition is specifically reversible by 0.1 mm l-methionine. in the same conditions even 1-2 mm mamp has a very slight effect on the growth rate of p. aeruginosa and only during the first two generations. the same obser ... | 1976 | 189717 |
| formation of a biologically active, ordered complex from two overlapping fragments of cytochrome c. | a noncovalent complex of the apoprotein (1-104) and cyanogen bromide heme fragment containing residues 1 to 65, (1-65) h, has been prepared from horse heart cytochrome c. conditions under which the redundant portions of the ferrous complex can be removed by limited trypsin digestion have been devised. the complementing fragments have been isolated from the derived complexes and four apofragments and one heme fragment have been identified in the amino acid sequence of cytochrome c. they are (39-1 ... | 1977 | 190231 |
| [microbial resistance to formaldehyde. i. comparative quantitative studies in some selected species of vegetative bacteria, bacterial spores, fungi, bacteriophages and viruses]. | the resistence of different microorganisms to formaldehyde was determined. as test objects served gram-negative and gram-positive vegetative germs (klebsiella pneumoniae, pseudomonas aeruginosa, salmonella paratyphi-b, staphylococcus aureus, streptococcus faecalis), bacterial spores (bacillus cereus, bacillus pumilus, bacillus stearothermophilus, bacillus subtilis), fungi (aspergillus niger, candida albicans), bacteriophages (escherichia coli phages, t1, t2, t3), and viruses (adenovirus, poliomy ... | 1976 | 190825 |
| inhibition of escherichia coli growth and respiration by polymyxin b covalently attached to agarose beads. | polymyxin b was attached to agarose beads by stable covalent bonds and the antimicrobial activity of the immobilized peptide was examined. polymyxin-agarose inhibited the growth of escherichia coli and pseudomonas aeruginosa, but not bacillus subtilis. in addition, the respiration of e. coli, e. coli spheroplasts, and b. subtilis protoplasts was inhibited by immobilized polymyxin, whereas the respiration of b. subtilis was unaffected by polymyxin-agarose. the activity of polymyxin-agarose was no ... | 1977 | 192271 |
| kinetic studies of the oxidation of horse heart ferrocytochrome c, pseudomonas aeruginosa ferrocytochrome c551, co(terpy)22+, and ru(nh3)5py2+ by tris(1,10-phenanthroline)cobalt(iii) ions. | | 1977 | 193881 |
| pyocin sensitivity of pseudomonas aeruginosa pretreated with antibiotics. | strains of pseudomonas aeruginosa exposed to subinhibitory concentrations of either polymyxin b, gentamicin, or carbenicillin were compared with untreated organisms for changes in sensitivity to pyocin. pretreatment with polymyxin b or gentamicin resulted in a decreased sensitivity to pyocin, while carbenicllin pretreatment did not alter pyocin sensitivity. | 1977 | 194663 |
| electron-transfer protein reactivities. kinetic studies of the oxidation of horse heart cytochrome c, chromatium vinosum high potential iron-sulfur protein, pseudomonas aeruginosa azurin, bean plastocyanin, and rhus vernicifera stellacyanin by pentaamminepyridineruthenium(iii). | | 1977 | 194940 |
| effects of lipopolysaccharide derived from pseudomonas aeruginosa on the size and cyclic nucleotide levels in human lymphocytes under the investigation by fluorescence-activated cell sorting. | | 1977 | 195110 |
| the reduction of pseudomonas cytochrome c551 oxidase by chromous ions. | the reduction of cytochrome c551 oxidase from pseudomonas aeruginosa by cr2+ ions was followed in the stopped-flow apparatus at a number of wavelengths. the c-haem reduction proceeded in a biphasic fashion with second-order rate constants of 2.6 x 10(5)m-1-s-1 and 4.8 x 10(4)m-1-s-1 at 25 degrees c, whereas the biphasic reduction of the d1-haem appeared to be independent of reductant concentration with rate constants of approx. 1.0s-1 and 0.25s-1 respectively. the kinetically determined differe ... | 1977 | 195577 |
| the oxidation of ferrocytochrome c in nonbinding buffer. | the apparent equilibrium constant and rate of oxidation was investigated for the reaction of cytochrome c with iron hexacyanide. it was found that if horse heart ferricytochrome c was exposed to ferricyanide (to oxidize traces of reduced protein) the cytochrome subsequently, even after extensive dialysis, had an apparent equilibrium constant different from that of electrodialyzed protein. the effect of ferricyanide ion apparently cannot be removed by ordinary dialysis. the ionic strength depende ... | 1977 | 196725 |
| immunological studies on the key enzyme of arginine biosynthesis in pseudomonas aeruginosa. | a method to manufacture specific antisera with a minute amount of prue enzyme is presented. the influence of antibodies on activity and inhibition of an allosterically regulated enzyme was studied. | 1977 | 196890 |
| nuclear-magnetic-resonance studies of pseudomonas aeruginosa cytochrome c-551. | nuclear magnetic resonance (nmr) spectroscopy was used to study pseudomonas aeruginosa cytochrome c-551. assignments of resonances to specific residues have been made. a low-resolution x-ray structure was used to aid assignments. a structural comparison was made between p. aeruginosa cytochrome c-551 and mammalian cytochrome c, based on comparisons of nmr data. | 1977 | 198212 |
| protocatechuate 3,4-dioxygenase. inhibitor studies and mechanistic implications. | protocatechuate 3,4-dioxygenase (ec 1.13.11.3) from pseudomonas aeruginosa catalyzes the cleavage of 3,4-dihydroxybenzoate (protocatechuate) into beta-carboxy-cis,cis-muconate. the inhibition constants, ki, of a series of substrate analogues were measured in order to assess the relative importance of the various functional groups on the substrate. though important for binding, the carboxylate group is not essential for activity. compounds with para hydroxy groups are better inhibitors than their ... | 1977 | 199266 |
| purification and some properties of cytochrome c-552 from an extreme thermophile, thermus thermophilus hb8. | a c-type cytochrome, cytochrome c-552, from a soluble fraction of an extreme thermophile, thermus thermophilus hb8, was highly purified and its properties investigated. the absorption peaks were at 552, 522, and 417 nm in the reduced form, and at 408 nm in the oxidized form. the isoelectric point was at ph 10.8, the midpoint redox potential was about +0.23 v, and the molecular weight was about 15,000. the cytochrome c-552 was highly thermoresistant. the cytochrome reacted rapidly with pseudomona ... | 1977 | 199583 |
| progressive necrotizing external otitis: treatment with ticarcillin and tobramycin. | the history of necrotizing external otitis, its diagnosis and management are reviewed. a case history is presented of a patient who was diagnosed as having progressive necrotizing external otitis with facial paralysis. in spite of standard medical treatment and aggressive surgical management, the disease process continued with progressive involvement of cranial nerves ix and x. the pseudomonas aeruginosa bacteria developed an increased minimal inhibitory concentration (mic) to carbenicillin and ... | 1977 | 199816 |
| anaerobes in human biliary tracts. | during a 2-year period, 1,892 patients underwent biliary tract surgery at the mayo clinic. both aerobic and anaerobic cultures of bile were performed in 371 of these patients. sixty-nine percent of the cultures were positive, and 41% (117) of these grew anaerobes, although they were present in pure culture only twice. mixed cultures most commonly contained four different organisms (three aerobes and one anaerobe). bacteroides fragilis was the single most commonly isolated anaerobe and ranked fou ... | 1977 | 200634 |
| in vivo activation by polymyxin b of phospholipase c from pseudomonas aeruginosa. | | 1977 | 201598 |
| reconstitution of the apoenzyme of cytochrome oxidase from pseudomonas aeruginosa with heme d1 and other heme groups. | cytochrome oxidase (ec 1.9.3.2) from pseudomonas aeruginosa contains heme d1 and heme c in an equimolar ratio. the heme d1 can be removed from the enzyme with acidified acetone leaving an apoenzyme that contains heme c but has no oxidase activity. reconstitution of the apoenzyme in neutral 6 m urea with heme d1 yields a reconstituted product which, after removal of the urea, has 90 to 100% of the oxidase activity of the native enzyme, a 1:1 molar ratio of the heme groups, and is indistinguishabl ... | 1978 | 201636 |
| the electron-transfer reaction between azurin and the cytochrome c oxidase from pseudomonas aeruginosa. | a stopped-flow investigation of the electron-transfer reaction between oxidized azurin and reduced pseudomonas aeruginosa cytochrome c-551 oxidase and between reduced azurin and oxidized ps. aeruginosa cytochrome c-551 oxidase was performed. electrons leave and enter the oxidase molecule via its haem c component, with the oxidation and reduction of the haem d1 occurring by internal electron transfer. the reaction mechanism in both directions is complex. in the direction of oxidase oxidation, two ... | 1977 | 202254 |
| structural homology of cytochromes c. | cytochromes c from many eukaryotic and diverse prokaryotic organisms have been investigated and compared using high-resolution nuclear magnetic resonance spectroscopy. resonances have been assigned to a large number of specific groups, mostly in the immediate environment of the heme. this information, together with sequence data, has allowed a comparison of the heme environment and protein conformation for these cytochromes. all mitochondrial cytochromes c are found to be very similar to the cyt ... | 1978 | 203462 |
| bactericidal action of ascorbic acid on psuedomonas aeruginosa: alteration of cell surface as a possible mechanism. | neutralised ascorbic acid is found to exert a strong bactericidal action on pseudomonas aeruginosa suspended in isotonic phosphate buffer at ph 7.1. both the bactericidal and bacteriostatic action of ascorbic acid are antagonised by magnesium ions. in the absence of complex formation between magnesium and ascorbic acid it is concluded that ascorbic acid acts by competing with the magnesium binding sites in the cell wall, cell membrane or ribosomes. using the chequer-board titration method the sy ... | 1978 | 207492 |
| synergistic infection with murine cytomegalovirus and pseudomonas aeruginosa in mice. | a previous report from this laboratory demonstrated that mice infected intraperitoneally with a 0--20% lethal inoculum of murine cytomegalovirus (cmv) exhibited markedly enhanced mortality rates (90%--100%) within 48 hr after an intravenous injection of a 0--20% lethal inoculum of pseudomonas aeruginosa. the current study demonstrated that mice infected with murine cmv alone had high titers of virus in multiple organs over a 20-day period, whereas mice injected with p. aeruginosa alone had a sel ... | 1978 | 207792 |
| inhibition of enzyme induction in pseudomonas aeruginosa by exogenous nucleotides. | alkylsufatase induction in resting cell suspensions of p. aeruginosa was inhibited by exogenously supplied adenosine or by atp (2mm). adenine phosphate had no effect while amp or adp caused a slight stimulation of induction. the inhibitory effect of atp required the presence of added mg2+, was not reversed by cyclic-amp (2mm), and was independent of the nature of the inducer. of a number of other nucleoside triphosphates tested, only utp (2mm) acted as an inhibitor of induction. these nucleotide ... | 1978 | 209878 |
| pseudomonas aeruginosa exoenzyme s: an adenosine diphosphate ribosyltransferase distinct from toxin a. | pseudomonas aeruginosa exoenzyme s is an adenosine diphosphate ribosyltransferase distinct from pseudomonas toxin a. exoenzyme s catalyzes the transfer of radioactivity from all portions of radiolabeled nad+ except nicotinamide. digestion of the radiolabeled product(s) formed in the presence of [adenine-14c]nad+ and exoenzyme s with snake venom phosphodiesterase yields only amp, suggesting that adp-ribose is present as monomers and not as poly(adp-ribose). exoenzyme s does not catalyze the trans ... | 1978 | 210453 |