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characterization of erythromycin resistance in campylobacter jejuni and campylobacter coli.the mechanism of resistance to erythromycin, the drug of choice in the treatment of campylobacter gastroenteritis, was investigated. erythromycin resistance (mics, greater than 1,024 micrograms/ml) in three clinical isolates of campylobacter jejuni and one c. coli isolate was determined to be constitutive and chromosomally mediated. in vivo protein synthesis in erythromycin-susceptible c. jejuni and c. coli strains was completely inhibited by low levels of erythromycin (5 micrograms/ml), whereas ...19911759819
emergence of fluoroquinolone resistance in campylobacter jejuni and campylobacter coli in subjects from finland.the in vitro susceptibilities of 102 human campylobacter strains isolated between 1978 and 1980 and 100 strains isolated in 1990 to ciprofloxacin, norfloxacin, erythromycin, gentamicin, and doxycycline were examined. the biotypes and heat-stable serotypes of the strains as well as antimicrobial treatments and travel history of the campylobacter-positive patients were also studied. the results indicated that susceptibility to erythromycin, gentamicin, and doxycycline has remained the same during ...19911759828
clinical and microbiological observations on cdc group df-3, a gram-negative coccobacillus.sequential stool cultures submitted for routine culture were screened for the presence of cdc group df-3. of 690 specimens, 11 (1.6%) yielded moderate to heavy growth of df-3. information on the 11 patients from whom these specimens were obtained showed that 4 had a history of prolonged diarrheal disease that resolved after specific therapy to eradicate df-3, while for the other 7 patients no clear role could be established. microbiological characterization of the stool isolates and 10 cdc strai ...19911761678
characterization of an immunoreactive species-specific 19-kilodalton outer membrane protein from helicobacter pylori by using a monoclonal antibody.immunoblotting experiments on hyperimmune rabbit serum and sera from patients with helicobacter pylori gastritis showed a consistent antibody response to a 19-kda outer membrane protein antigen. a monoclonal antibody, designated hp 40, which reacted by western immunoblotting with this protein was produced. it was shared by all h. pylori strains tested (d 273, nctc 11637, and 24 wild strains) but not by the thermophilic campylobacter species, campylobacter fetus, helicobacter mustellae, or helico ...19911761682
[a clinical experience of rokitamycin on campylobacter enteritis. research group of rokitamycin on infectious enteritis].rokitamycin, a newly developed macrolide, was administered to a total of 107 cases, 16 years old or more, in order to evaluate its clinical efficacy, safety and usefulness on campylobacter enteritis. daily dosage of 600 mg of rokitamycin was administered orally in three divided doses for 5 days. bacteriological and clinical efficacies were judged by the attending doctors from the evaluation criteria made by the committee and from the days required for improvement of diarrhea, defervescence and s ...19911761895
[comparison of clinical efficacy of rokitamycin (rkm) and ofloxacin (oflx) for the treatment of campylobacter enteritis by a double-blind method. the research committee for the effect of rokitamycin, research group for infectious enteritis].the clinical efficacy, safety and usefulness of rokitamycin (rkm), a new macrolide antibiotic, were compared with those of ofloxacin (oflx) for the treatment of campylobacter enteritis by a double blind method. the daily dose level of rkm or oflx was 600 mg. they were orally administered in three divided doses for 5 days. of 223 cases studied, 106 cases were diagnosed as campylobacter enteritis. ninety cases (rkm group: 50, oflx group: 40) except for 16 excluded or drop-out cases were analysed. ...19911761896
campylobacter fetus subspecies fetus infection.during a six-year period five patients with campylobacter fetus subspecies fetus infections were seen at the mayo clinic in rochester, minnesota. bacteremia was observed in two patients, one presenting with aortic valve endocarditis and the other with abdominal atherosclerotic aortic aneurysm. c. fetus subsp. fetus was isolated from tibial tissue of a patient with osteomyelitis. diarrhea was the main complaint of two further patients, and was also mentioned by the patient with the aortic aneurys ...19911762387
prevalence of bovine campylobacteriosis in indigenous cattle of three states in nigeria.a survey of bovine campylobacteriosis in breeding bulls and cows was carried out in the states of kaduna, kano and borno. six hundred and eighty nine cattle composed of 585 and 104 breeding bulls and cows respectively were sampled. campylobacter fetus subsp. venerealis was isolated from 12 bulls while campylobacter fetus subsp. fetus was isolated from three of them. campylobacter fetus subsp. fetus was isolated from four cows while campylobacter fetus subsp. veneralis was isolated from one cow. ...19911763437
gastroenterological emergencies in the tropics.significant differences exist in the prevalence of most gastroenterological emergencies in tropical compared with temperate countries. both ethnic and environmental (often clearly defined geographically) factors are relevant. the major oesophageal lesions which can present acutely in tropical countries are varices and carcinoma; bleeding and obstruction are important sequelae. peptic ulcer disease (and its complications), often associated (not necessarily causally) with helicobacter pylori infec ...19911764626
[guillain-barré syndrome preceded by diarrhea with the infection of campylobacter jejuni].we report a 77-year-old woman with guillain-barré syndrome following campylobacter jejuni infection. she was admitted complaining of mild weakness in the left leg. seven days before, she had severe diarrhea, which continued several days. after admission, the weakness soon worsened resulting in tetraparesis, and the respiration became so impaired that she was supported by the artificial ventilator. deep tendon reflexes were absent in four limbs, and no sensory disturbance was noted. csf contained ...19911764866
[significance of campylobacter pylori in the surgical treatment of stomach and duodenal ulcer]. 19911767324
mass screening of cattle sera against 14 infectious disease agents, using an elisa system for monitoring health in livestock.mass screening elisa methods were developed for testing cattle serum for antibodies against 14 common livestock diseases simultaneously. the absorbance values were transformed to a %elisa (spectrophotometric antibody end point) by a computer interfaced with a microplate reader. a histogram indicating a cutoff point and a report for the veterinarian also was generated. the computer program produced a print-out of the antibody profile for each animal tested, the antibody concentration against each ...19911767993
rheumatic symptoms following an outbreak of campylobacter enteritis: a five year follow up.eighty six of 106 (81%) guests attending a party were followed up after an outbreak of campylobacter jejuni enterocolitis. acute diarrhoeal illness was reported in 35 subjects (33%), of whom seven showed acute rheumatic symptoms either alone or with other symptoms of infection with c jejuni. the antibody response to c jejuni corresponded well with the intensity of the disease. in the early phase of the gastrointestinal disease the patients with acute rheumatic symptoms displayed significantly hi ...19911768164
[helicobacter (campylobacter) pylori in stomach and duodenal biopsies].in bioptic specimens of the gastric mucosa of 57 patients with dyspeptic complaints and/or a duodenal ulcer helicobacter pylori was detected under the microscope in 82,4% of chronic active gastritis and in 28,6% and 61,5% resp. of chronic gastritis grade i and ii. the finding of helicobacteria depended on the number of collected specimens. they were never found at sites with intestinal metaplasia and their numbers did not correspond to the intensity and character of the inflammatory changes. as ...19911769044
genomic investigation of phenotypic variation in campylobacter jejuni flagellin.western blots of whole-cell sonicates of 10 different clones of a faecal isolate of campylobacter jejuni 533 detected the expression of flagella antigens of either 59 or 62 kda. other antigenic proteins appeared identical both in the parent and all the clones. the mechanism for this phenotypic variation was studied using southern blotting with a flagellin-specific gene probe and products of a polymerase chain reaction (pcr) using flagellin-gene primers. restriction-enzyme digestion and southern ...19911769546
recovery of viable but non-culturable campylobacter jejuni.suspensions of campylobacter jejuni became non-culturable after storage in sterilized pond water at 4 degrees c for periods between 18 and 28 d, depending on the strain. suspensions of four strains of c. jejuni that had been in water for 6 weeks, and shown to be non-culturable, were fed to suckling mice. colonization of mice was established with two of the strains and failed with the other two strains. examination of these suspensions under the electron microscope showed some cocci having the ap ...19911770362
[chronic active surface-positive campylobacter antrum gastritis-- morphological changes 1 year after treatment with bismuth].the works is based on a previous investigation published in the journal vnitrní lékarství, 36, 1990, 8, pp. 759-762. the authors investigated morphological changes of the gastric mucosa and the presence of campylobacter pylori (cp) in probands with chronic active surface cp positive antrum gastritis one year after treatment with a bismuth preparation (bismuth citrate 120 mg per capsule made on prescription) for a one-month period 4 capsules per day. this concerned probands with functional dyspep ...19911771809
[microbiologic findings in protracted and chronic enterocolitis].novel diagnostic methods (immunoblotting, immunofluorescence test) have been proved to be very sensitive and specific for detecting enteropathogenic bacilli. 28 patients with chronic enterocolitis were investigated. in 50% (14/28) of these patients yersinia enterocolitica could be visualized in biopsies by immunofluorescence. the serum of 8 patients also contained iga-antibodies against plasmid encoded released proteins of y. enterocolitica. this paper reports evidence for persistence of yersini ...19911771939
analysis of the role of flagella in the heat-labile lior serotyping scheme of thermophilic campylobacters by mutant allele exchange.flagellin mutations originally constructed in campylobacter coli vc167 (serotype lio8) by a gene replacement mutagenesis technique (p. guerry, s. m. logan, s. thornton, and t. j. trust, j. bacteriol. 172:1853-1860, 1990) were moved from the original host into campylobacter strains of a number of other lior serogroups by a natural transformation procedure. this is the first report of the use of this transformation method to transfer a mutated locus among campylobacter strains. flagellin mutants w ...19911774247
comparative evaluation of three selective media and a nonselective medium for the culture of helicobacter pylori from gastric biopsies.plating on solid media is the standard technique used in most laboratories for the isolation of helicobacter pylori from gastric biopsies. recently, various selective media were developed for this purpose. we compared and evaluated three selective media, skirrow's, dent's cp, and modified glupczynski's brussels campylobacter charcoal media, and chocolate agar medium for the isolation of h. pylori. gastric biopsies taken from a total of 203 patients were plated in parallel on all four media. an i ...19911774265
isolation of campylobacter spp. from stool specimens with a semisolid medium. 19911774290
reproducibility of tolerance tests that are useful in the identification of campylobacteria.twenty type or other reference strains, each representing a different campylobacter, helicobacter, or arcobacter taxon, were used to assess the reproducibility of 25 phenotypic tests that are used in the identification of such organisms. twenty-two of the tests depended on growth inhibition, and each of these tolerance tests was performed by using three different basal media. although the overall reproducibility of the tests with each basal medium exceeded 89%, the proportion of strains that wer ...19911774297
incidence of campylobacter pylori in a consecutive series of surgical patients referred for endoscopy. 19911774718
nonsyphilitic spirochetosis in second-trimester fetuses.four female fetuses (17-23 weeks) spontaneously aborted by young women (15-19 years old) showed spirochetal microorganisms predominantly in the intestinal lumen and mucosa and to a much lesser extent in other organs. fetal tissues showed a brisk lymphocytic-plasmacytic response in intestinal mucosa, lungs, and meninges in some cases. in all instances the placenta had chorioamnionitis and severe chronic villitis, with villous vasculitis in some. one fetus had a concomitant cytomegalovirus infecti ...19911775399
[campylobacter laridis: first clinical isolation and identification of a reservoir in chile]. 19901775794
use of ciprofloxacin for successful eradication of bacteremia due to campylobacter cinaedi in a human immunodeficiency virus-infected person.a 36-year-old homosexual man who was infected with human immunodeficiency virus presented with a 2-month history of fever and intermittent diarrhea. stool cultures were negative for bacterial pathogens, ova, parasites, and acid-fast organisms. an initial blood culture became positive after 5 days for a curved, gram-negative rod that was identified later as campylobacter cinaedi. the patient received a series of antibiotic regimens, including a 2-week course of erythromycin followed by a 2-week c ...19911775838
comparison of surface antigens of some campylobacter fetus subsp. fetus strains of ovine origin by polyacrylamide gel electrophoresis and immunoblotting.sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblotting were used to identify and to compare the surface antigens of eight c. fetus subsp. fetus strains. seven strains (one of serogroup a and six of serogroup b) were isolated from aborted ovine fetuses, while one strain (serogroup a) originated from an aborted calf fetus. saline extracts at 56 degrees c and 100 degrees c were used as antigens. antisera were produced in rabbits. in saline extracts (56 degrees c) of ...19911776377
the barren camel with endometritis--isolation of trichomonas fetus and different bacteria.this study constitutes the first reported isolation of trichomonas fetus from the uterus of breeding camels. twenty-four of 68 camels, which were barren for 1-4 years were found to be positive for t. fetus. one preputial washing of a camel bull which had served the herd was found to be positive for t. fetus. the uterine flora of all camels were examined. ten different bacterial and 2 fungal species were isolated. all samples were negative for campylobacter sp., taylorella equigenitalis and strep ...19911776379
modification to the ritard surgical model.the removable intestinal tie-adult rabbit diarrhea (ritard) procedure is the surgical model of choice for studying pathogenesis of diarrheas of campylobacter, escherichia coli, and aeromonas origin. other than nonhuman primates, the ritard rabbit is the only model that closely resembles human infection. classically, the ritard model is created by isolating the terminal ileum using umbilical tape placed in a slipknot fashion, and injecting the occluded segment with the organism of choice. the sli ...19911777445
survival and injury of escherichia coli, campylobacter jejuni, and yersinia enterocolitica in stream water.experiments were done to describe the survival and injury of three strains each of enteropathogenic escherichia coli, yersinia enterocolitica, and campylobacter jejuni in agricultural surface water. bacterial suspensions within membrane diffusion chambers were immersed in two large vessels of stream water that were held at 6 and 16 degrees c and changed daily. the results of daily plate counts, using selective and nonselective media, revealed some variation among strains and genera. injury incre ...19911777856
development of spontaneous resistance to ciprofloxacin in a strain of campylobacter jejuni. 19911778865
persistent diarrhea: total gut transit time and its relationship with nutrient absorption and clinical response.the study was undertaken to better understand the role of total gut transit time (tgtt) on the absorption of nutrients in patients with persistent diarrhea. twenty-six boys aged 3-18 months with persistent diarrhea and 25 age-matched healthy controls were studied. their tgtt was measured with charcoal markers during their treatment with a diet made up with rice powder soya oil, glucose, and egg white. coefficients of absorption of nutrients were estimated in a 72-h balance study. the median tgtt ...19911779316
diarrhoeal disease in children less than one year of age at a children's hospital in guangzhou, people's republic of china.we performed a case-control study of diarrhoea to determine its causes in children less than 1 year old in guangzhou, people's republic of china, in april to september 1989. stools were cultured for salmonella, shigella, campylobacter and vibrios by standard techniques; rotavirus (rv) was identified by polyacrylamide gel electrophoresis; and specific deoxyribonucleic acid probes were used to identify escherichia coli containing genes coding for shiga-like toxin i and ii, enteropathogenic e. coli ...19911781004
recovery of injured campylobacter jejuni cells after animal passage.sixteen freeze-thaw-injured nonculturable stocks of campylobacter jejuni were passed through rat gut, and seven were reisolated. these reisolated strains were converted to toxin producers, as they were before preservation, following consecutive passages through rat gut. this observation indicated the existence of an injured, viable, but nonresuscitated form of c. jejuni which can be resuscitated to a culturable and fully virulent form by passaging the organism through a susceptible host.19911781696
electron microscopic appearance of the chronic campylobacter jejuni enteritis of mice.campylobacter jejuni is a major cause of human enteritis which mimics the inflammatory bowel disease (ibd). in this study, microstructural changes on the surfaces of the murine gastrointestinal tract persistently colonized by campylobacter jejuni, strain gj-s131, were investigated by using scanning electron microscopy (sem) and transmission electron microscopy (tem). the results revealed that the appearance of the gastrointestinal mucosa in both balb/c and km mice resembled that in human with in ...19911782809
isolation of campylobacter from livers of broiler chickens with and without necrotic hepatitis lesions.injured and normal livers from broiler chickens sent to slaughter plants were collected for bacterial examination. a total of 223 macroscopically abnormal livers and 50 normal livers were received. forty-seven thermophilic campylobacter isolates were obtained from the livers with necrotic lesions; 39 isolates were identified as campylobacter jejuni and eight as c. coli. in normal livers, six c. jejuni isolates were obtained. c. jejuni biotype 2 was the most common isolate recovered from injured ...19911786003
phagocytosis and intracellular killing of campylobacter jejuni by elicited chicken peritoneal macrophages.in vitro phagocytosis and intracellular survival of campylobacter jejuni (strains b540 and clin 1) in chicken peritoneal macrophages were studied. macrophages were induced with sephadex g-50 and harvested 48 hr later by peritoneal lavage. the extent of phagocytosis over time was determined by enumerating the intracellular c. jejuni after removal of extracellular c. jejuni with gentamicin. pre-incubation of c. jejuni with antiserum generally enabled the macrophages to ingest greater numbers of ce ...19911786008
childhood diarrhoea in a low-income urban community in bangkok: incidence, clinical features, and child caretaker's behaviours.a one-year surveillance study of childhood diarrhoea in a low-income urban community in bangkok revealed an annual incidence of 2.2 episodes per child among infants, and that the overall annual incidence among children under five years of age was 0.9 per child. rotavirus, salmonella and campylobacter jejuni were common aetiologic agents. in children less than one year, diarrhoea was caused mostly by rotavirus and salmonella. in 1-2 year old children, the major causative agent was rotavirus while ...19911787280
[campylobacteriosis in clinical practice]. 19911787672
skirrow agar for simultaneous isolation of brucella and campylobacter species. species. 19911788919
[isolation and identification of bacteria of the genera campylobacter and helicobacter]. 19911789898
[in vitro antimicrobial activity of rokitamycin, a macrolide antibacterial agent, against clinically isolated strains of campylobacter and other enteritis-causing bacteria].we determined the minimum inhibitory concentrations (mics) of rokitamycin (tms-19-q, rkm), a macrolide antimicrobial agent, against strains of various bacterial species isolated from enteritis patients, and compared them with those of josamycin (jm), erythromycin (em) and ofloxacin (oflx). mic90 of rkm against 147 strains of campylobacter jejuni, and each 25 strains of shigella spp., salmonella spp. and diarrheagenic escherichia coli were 1.56, 200, 800 and 200 micrograms/ml, respectively. there ...19911791330
[current developments in the use of intravenous immunoglobulins].the use of intravenous immunoglobulins (ivig) has provided a substantial therapeutic advance in the treatment of antibody deficiency syndromes improving the quality of life of the affected patients. among the advantages there are the possibility to employ higher doses to reach higher serum levels, with a more efficacious prophylaxis against infections; the prevention of chronic lung disease if ivig are prescribed early, at diagnosis or at least at the onset of symptoms; the significant improveme ...19911791793
comparison and modification of rrna sequencing methods.in this study, modification of two methods of rna sequencing resulted in more definitive sequencing bands. in one method of sequencing, the bands of lane a and lane g sometimes were not clear. modifications of this method by changing the concentrations of ddatp and ddgtp resulted in the bands of lane a and lane g becoming more readable. although a second sequencing method was found to have clearer bands than the first method, and the bases immediately downstream from the primer binding site coul ...19911793573
development of resistance to quinolones in five patients with campylobacteriosis treated with norfloxacin or ciprofloxacin.development of resistance to nalidixic acid, norfloxacin and ciprofloxacin was observed in five patients with campylobacter jejuni or campylobacter coli infection. from all these patients nalidixic acid- and quinolone-susceptible strains were isolated initially, whereas after therapy with norfloxacin or ciprofloxacin strains resistant to these antibiotics were found. campylobacter strains from the same patient always belonged to the same species and, with the exception of one case, showed identi ...19911794366
gastritis and helicobacter (campylobacter) pylori--merely one more piece in the jigsaw puzzle or the final answer? 19911794913
campylobacter spondylodiscitis and deficiency of cellular immunity.we describe a case of spondylodiscitis caused by campylobacter fetus in a previously healthy patient. this is the first report of this agent causing discovertebral infection, which generated cellular immune deficiency, reversible with the cure.19911795335
[bacterial ileocecitis: a "new" disease].in cases of clinical suspicion of an acute appendicitis sonography uncovers another disease as the real cause of the symptoms in about one-quarter of such cases. bacterial ileocaecitis is most frequently diagnosed (11.6% of n = 786). in special bacteriological stool cultures, yersinia enterocolitica and campylobacter jejuni were identified, whereas salmonella enteritidis was a rarer finding. the typical sonographic manifestation of bacterial ileocaecitis compared against crohn's disease of the i ...19911796286
evaluation of culture media and antimicrobial susceptibility of helicobacter pylori.isolation of helicobacter pylori on artificial culture is hampered by the lack of reliable and cheap media. in this study, three different types of culture media were evaluated for isolation of h. pylori from clinical specimens. these media included: modified thayer-martin (mtm), skirrow's campylobacter agar and chocolate agar. modified thayer-martin agar was superior in isolation to others with an isolation rate of 47% (31/66). the size of colonies on this media were larger and clearly defined. ...19911800079
a study of the bacterial and parasitic causes of acute diarrhoea in northern jordan.the prevalence of bacterial and parasitic diarrhoeagenic agents in 200 patients suffering from acute diarrhoea and reporting to hospitals and health centres in northern jordan, and in controls was determined. one or more bacterial or parasitic enteropathogens was isolated from 79 patients (39.5%). prevalence rates for these pathogens was as follows: enterotoxigenic escherichia coli, 9%; enteropathogenic e. coli, 9%; salmonella spp. 7%; campylobacter spp, 5.5%; yersinia enterocolitica, 4.5%; shig ...19911800560
campylobacter and enterohaemorrhagic escherichia coli (ehec) associated gastroenteritis in turkish children.from may 1989 to april 1990, stool specimens of 677 children less than 15-years old who were admitted to the hacettepe university outpatient department of pediatrics with acute enteritis were collected to search for four common enteric pathogens: salmonella, shigella, campylobacter, and enterohaemorrhagic escherichia coli (ehec). at least one of the three bacteria was detected in 107 (15.8%) of the patients. campylobacter was found in 72 (10.6%) of the samples. shigella and salmonella spp. were ...19911800562
acute diarrhoeal diseases in infants aged below six months in hospital in calcutta, india: an aetiological study.in a prospective 2-year study, 218 infants aged less than 6 months admitted to a children's hospital in calcutta with acute diarrhoea and 102 infants (control group) from the out-patient department of the same hospital with similar age, sex and socio-economic composition as the diarrhoea patients, were concurrently investigated for enteropathogenic agents in the faeces. enteropathogenic escherichia coli, enterotoxigenic e. coli, and enteroadherent e. coli constituted the 3 types of diarrhoeageni ...19911801358
[an immunoenzyme test system for the detection of antibodies to campylobacter].an enzyme immunoassay system for the detection of antibodies to bacteria of the genus campylobacter in human blood serum has been developed. the system is based on the use of ethanol-treated c. jejuni and c. coli whole cells as antigen. the study of sera obtained from healthy donors in this assay has made it possible to establish the value of the tentative diagnostic titer: 320.19911801490
[the adhesive properties of bacteria of intestinal origin].differences between strains of nonpathogenic escherichia and lactobacilli, as well as some pathogenic bacteria of enteric origin (escherichia, shigella, campylobacter), in their capacity to adhesion to rat enteric and colonic cells have been shown in vitro. the strains under study have been found to possess more pronounced adhesiveness with respect to colonic cells, which is indicative of their higher receptive capacity in comparison with enteric cells. in the absence of normal microflora lactob ...19911801491
[campylobacter jejuni--the etiologic agent of enteritis; the research experience within a closed collective of infants]. 19911802289
campylobacter jejuni infected bursitis.campylobacter jejuni is a common cause of enteritis, and has been isolated from patients with bacteremia, meningitis, and cholecystitis. we describe here an unusual case of a chronically inflamed bursitis infected with c. jejuni.19911802538
[the role of serology in the diagnosis of helicobacter (campylobacter) pylori infection].the purpose of the communication is to review the different aspects of the helicobacter (campylobacter) pylori infection. the first part of the communication is devoted to the description of the different gastric pathologies induced by the helicobacter pylori infection and to the different methods used for the detection of this infection. today a consensus assesses a causal role to helicobacter pylori in the development of chronic active gastritis (or type b gastritis), in the pathogenesis of du ...19911803838
cryptosporidium sp. in stool specimens from diarrhoeic and asymptomatic individuals in the magdeburg area (east germany).unselected stool specimens from a total of 2,944 individuals with diarrhoea including 1,172 children under 14 years of age were investigated for cryptosporidium oocysts in the magdeburg area from 1987 to 1988, 43 (1.46%) were found positive. three of these were additionally infected with bacterial pathogens (campylobacter species). in all cases the symptoms of diarrhoea ceased spontaneously after an average of 8 days. the incidence was highest among children and infants under 6 years of age (2.5 ...19911803951
[morphological changes of campylobacter jejuni and campylobacter coli under various culture conditions and the accompanied changes in the cell composition].the morphological transformation from the spiral form to the coccoidal form campylobacter jejuni and campylobacter coli was studied under various conditions by such techniques as electron microscopy, sds-polyacrylamide gel electrophoresis (sds-page), and chemical analyses. the conversion from the spiral form to the coccoidal form of campylobacter in microaerophilic cultivation occurred in about 50% of the cells in 48 h and in about 90% of the cells in 72 h. at higher temperatures (37 c and 42 c) ...19911805043
[development of gentamicin sulfate sustained release tablet remaining-floating in stomach].gentamicin sulfate sustained-release tablet remaining-floating in stomach (g-hbs) was developed based on the hydrodynamically balanced system. the dissolution rate of g-hbs was determined by rotary basket method (100 r/min, 37 +/- 0.5 degrees c, 0.1 mol/l hcl). the release characteristics of g-hbs showed basically first order kinetics with the dissolution rate constant (kr) of 0.3992 h-1. the mean dissolution time (mdt) of g-hbs was 2.53 h-1. the density of g-hbs was found to have no significant ...19911805513
[the effects of some technologies on survival of campylobacter jejuni in food of animal origin].the effects of some technologies (cooling, freezing, salting, packaging, fermentation, cold smoking, heat treatment) on the survival of campylobacter jejuni were investigated. the different technological procedures can be considered as barriers influencing to a certain degree the survival of c. jejuni. the low value aw on the surface of chilled meat and oxygen presence reduce the occurrence of c. jejuni, but the low storage temperature prolongs the survival of c. jejuni to two days. in chilled p ...19911807034
[an unusual cause of non-lithiasic acute cholecystitis: campylobacter jejuni]. 19911809764
alternative sigma factors and the regulation of flagellar gene expression.synthesis of bacterial flagella and the accompanying array of chemotaxis receptors and transducers represents a major commitment of energy and resources for a growing bacterial cell and is subject to numerous levels of regulation. genes for flagellar and chemotaxis proteins are expressed in a complex transcriptional cascade. this regulatory hierarchy acts to ensure that the highly expressed filament structural protein, flagellin, is synthesized only after a prerequisite set of structural protein ...19911809831
causative role of yersinia and other enteric pathogens in the appendicular syndrome.in 2,861 consecutive patients undergoing appendicectomy for clinically suspected appendicitis an enteric pathogen was isolated from the appendix in almost 7% using an optimal combination of culture media. the pathogenic yersinia enterocolitica serotypes 03 and 09 predominated (3.6%), followed by campylobacter and nontyphoid salmonella. the same pathogen was isolated from the stool in 72.5% of patients with a culture-positive appendix and in 84.1% of those positive for a pathogenic yersinia. conv ...19911810725
[campylobacter pylori gastroduodenitis in children with juvenile rheumatoid arthritis].clinico-morphological study of the gastroduodenal system and also morphometric investigations of the degree of gastroduodenitis activity and the condition of the local immune system were made in 30 children aged from 4 to 15 years with juvenile rheumatoid arthritis (jra). microbiological, histological and electron microscopic methods for revealing campylobacter pylori (cp) were used. interrelationship between various clinical manifestations of jra with the nature of gastroduodenal system affecti ...19911812557
anti-helicobacter pylori igg levels in abattoir workers.the aim of our study was to detect antibodies against helicobacter pylori (h. pylori) in abattoir workers whose activity was variable (pig, poultry-carcasses, poultry-intestinal organs treatment). an adsorption of all sera with h. pylori and campylobacter species known to be ubiquitous and responsible for zoonosis was included in the elisa reaction to eliminate possible cross-reactions between these species. statistically higher specific titres of igg antibodies against h. pylori were observed i ...19911816012
prevalence of campylobacter pylori in non-ulcerative dyspepsia.endoscopic biopsies were taken from the gastric antral mucosa, in 150 cases of non-ulcerative dyspepsia at the gastroscopic clinic, dist. hospital, belgaum. spiral or curved bacilli, were demonstrated in specimens from 99 patients. the histologic demonstration of the organism by warthin starry stain (66%) was superior to haematoxylin and eosin (47.33%), urease test (42%) and gram's stain (35.33%). there was a strong association between c pyloridis in the gastric mucosa and histologically defined ...19911818028
the effect of lactic acid sprays on campylobacter jejuni inoculated onto poultry carcasses.spraying poultry carcasses with 1% lactic acid 10 min after inoculation with campylobacter jejuni, resulted in a significant reduction in the number of the bacteria after 4 h at 4 degrees c. some of the inoculated cells, however, survived for at least 144 h. spraying 10 min after inoculation with 2% lactic acid, totally eliminated all inoculated c. jejuni within 24 h. on the other hand, spraying 24 h after inoculation, with either 1% or 2% lactic acid did not eliminate all the bacteria. inoculat ...19911818508
[resistance to chemotherapy of campylobacter jejuni strains].sensitivity to 22 different chemotherapeutic agents against 31 campylobacter jejuni strains, isolated from children was determined. most of the investigated strains were sensitive to doxycycline, chloramphenicol and nitrofurantoin (90-100) and 84-87% of them to oxy-tetracycline augmentation, ofloxacin, erythromycin. all the strains were resistance to: cephalotin, cephadrin, cefuroxime and cefoperazone and 77-87% to ceftazidime, colistin and vancomycin.19911819812
[cidade leonor slum, são paulo. iii. environmental contamination and natural feeding practice].in this study the authors report the high rate of environmental contamination to which children living in slum areas are exposed. the authors performed a bacteriologic and parasitologic survey in children with acute diarrhea and in controls. feeding habits were also recorded. the feeding habits revealed that 176 out of 520 children (33.8%) were never breast fed, whereas the remaining 344 (66.7%) were breast fed for an average of 2.2 months. the parasitologic survey was positive in 89% of the sto ...19911820601
use of ampicillin plus ranitidine for helicobacter pylori gastritis.1. helicobacter pylori (formerly campylobacter pylori) is now recognized as an etiological factor in gastritis and duodenal ulcers and probably also gastric ulcers. eradication of the bacteria is fundamental to avoid ulcer relapse. although bismuth salts have been shown to be effective for treatment, they are not commercially available in brazil. 2. we report an attempt to treat patients with helicobacter pylori-associated gastritis with ampicillin (1000 mg twice daily for one month) and compare ...19911823272
oil immersion for identification of campylobacter pylori. 19911824230
[neonatal infection caused by campylobacter jejuni]. 19911826359
the development of macrolides: clarithromycin in perspective.macrolide antibiotics have been available and used clinically since 1952. the class of drugs originated from a soil sample obtained from the city of ilo-ilo on the island of paray in the philippines. erythromycin has been the most widely used agent of this class called 'macrolides' because they possess the macrocyclic lactone nucleus. many esters of erythromycin are well established as agents to treat a variety of respiratory and cutaneous infections, particularly in children. there has been a r ...19911827094
a noti restriction site of campylobacter coli chromosomal dna detected by double-digestion analysis with apai. 19911832048
[the epidemiological aspects of campylobacteriosis in children in arid area].specific epidemiological features of campylobacter infection in children in samarkand province during the period of 1987-1990 are discussed. the specific proportion of this infection in the total structure of acute enteric infections was, on the average, 8.5%, and campylobacter carriership among healthy children, 7.0%. among children with campylobacter infection, children aged up to 1 year constituted 71.5%. the results of the study of the biological properties of campylobacter strains isolated ...19911838656
[risk factors and transmission routes of the causative agents of campylobacteriosis in commercial poultry plants].the study, carried out in two regions of the ussr and aimed at estimation of the contamination of products supplied by industrial poultry complexes (ipc), revealed that the contamination of these products was closely related to the campylobacter contamination of the personnel of ipc. the causes of high campylobacter contamination of the products of ipc at all technological stages of their production are described. the species, serovars and biovars of campylobacter strains isolated from different ...19911838657
campylobacter intestinal carriage among stray and pet dogs.the natural distribution of thermotolerant campylobacter sp. in dogs (150 stray animals and 64 pets) was studied. campylobacters were more frequently isolated (p < 0.01) from stray dogs (51.3%) rather than from pet dogs (21.9%). all the biotypes described by lior for c. jejuni and c. coli were found among stray animals, whereas only c. jejuni biotypes i and ii and c. coli biotype ii were found among pet dogs. the need for more studies related to the role of environmental sanitary conditions in t ...19911843237
diagnosis of helicobacter pylori: comparison of an urease test, histological visualization of curved bacteria and culture.helicobacter pylori was investigated in 189 patients for culture, microscopic visualization of campylobacter-like organisms (clo) and a ten minute urease test. in 136 (72%) the bacteria was isolated, and in 98 of them clo were histologically detected. specificity, sensitivity, positive and negative predictive values of microscopic visualization of clo were: 0.77, 0.73, 0.97 and 0.51, respectively; 98 culture-positive patients were urease test positive. specificity, sensitivity, positive and nega ...19911843403
development of epidemiological method for the helicobacter pylori by polymerase chain reaction.the polymerase chain reaction was used to develop a method for the detection of helicobacter pylori, a causative agent of gastritis, as well as for the elucidation of its mode of transmission. a genomic library of helicobacter pylori dna in escherichia coli jm109 was constructed by cloning hind iii-digested dna fragments into plasmid vector puc18. the nucleotide sequences from seven recombinant clones were determined and five sets of oligonucleotide primers were synthesized on the basis of the s ...19911844643
[occurrence of campylobacter pylori in gastric mucosa and selected parameters of cell-mediated immunity in patients with duodenal ulcer and individuals with non-ulcerative dyspepsia].the study was aimed at investigating a relationship between campylobacter pylori infection in the gastric mucosa and selected parameters of cell-mediated immunity in patients with duodenal ulcer and the individuals with non-ulcerative dyspepsia. a relationship between campylobacter pylori and gastritis has also been studied. endoscopic and immunological tests were carried out in the group of 45 patients, including 14 patients with duodenal ulcer and 29 with non-ulcerative dyspepsia. specimens of ...19911845737
role of the beta-lactamase of campylobacter jejuni in resistance to beta-lactam agents.we studied the role of the beta-lactamase of campylobacter jejuni in resistance to beta-lactam agents. beta-lactamase-positive strains were more resistant than beta-lactamase-negative strains to amoxicillin, ampicillin, and ticarcillin (p less than 0.05). with penicillin g, piperacillin, imipenem, and six cephalosporins, the susceptibility levels were similar for both beta-lactamase-positive and -negative strains. by using spectrophotometric and microbiological assays, the beta-lactamase from th ...19911854162
wolinella recta, wolinella curva, bacteroides ureolyticus, and bacteroides gracilis are microaerophiles, not anaerobes.although the nonfermentative, asaccharolytic, putative anaerobes wolinella curva, wolinella recta, bacteroides ureolyticus, and bacteroides gracilis are phylogenetically related to the true campylobacters, the type strains of these species exhibited o2-dependent microaerophilic growth in brucella broth and on brucella agar. the optimum o2 levels for growth of these strains ranged from 4 to 14% in brucella broth and from 2 to 8% on brucella agar, when h2 was provided as the electron donor. no gro ...19911854636
enterotoxigenic campylobacter jejuni & c. coli in the etiology of diarrhoea in northern india.faecal specimens from subjects with (320) and without (450) diarrhoea were screened for campylobacter jejuni and c. coli. c. jejuni and c. coli were detected in 5 per cent of subjects with diarrhoea and 0.7 per cent of those without diarrhoea and the difference was significant (p less than or equal to 0.01). the isolation rate was much higher in under five diarrhoeal children (8.3%), in comparison to the older group (3.0%). eleven diarrhoeal isolates of c. jejuni and c. coli were tested in rat i ...19911855823
simplified method for the detection of dna hydrolysis by enteric campylobacters.a simplified medium was developed for the detection of dnase produced by enteric campylobacters. sensitivity and reproducibility of the test were similar to that of the improved toluidine blue dna agar method. logistically, the simplified dna hydrolysis test was cheaper (5.5 times) than the earlier medium. based on this study we recommend the routine use of the simplified medium to perform the dnase test for biotyping enteric campylobacters.19911855824
exposure to n-nitrosamines and other risk factors for gastric cancer in costa rican children.the hypothesis that endogenous chemical nitrosation in the normal stomach in early life could play a crucial role in inducing chronic atrophic gastritis/intestinal metaplasia in later life was tested by applying the n-nitrosoproline (npro) test to 12-h urine samples from about 50 children (aged 8-14 years) living in high- and low-risk areas for stomach cancer. the median values of npro and the sum of four nitrosamino acids analysed were 0.28-0.84 micrograms/12 h and 0.75-1.75 micrograms/12 h, re ...19911855842
the etiology of gastric cancer.we review recent evidence concerning risk factors for gastric cancer. an overview of analytical studies shows convincing evidence of a protective effect of fruit and vegetables. the specific protective constituents have not been firmly established, but micronutrients, especially ascorbic acid, are probably of importance. other dietary factors that show a consistent pattern of effect in different studies are the moderate risks associated with high intake of preserved foods and salt. evidence also ...19911855854
identification of campylobacter jejuni and campylobacter coli antigens with mucosal and systemic antibodies.the development of a rapid and specific diagnostic assay for campylobacter infections is important in determining the etiology of acute diarrhea in humans. studies have shown that sonicated whole bacteria or partially purified antigens cross-reacted with antibodies against other closely related bacteria. to solve the problems of specificity, we identified specific antigens of campylobacter jejuni and campylobacter coli for use in diagnostic assays. we investigated the responses of serum, urine, ...19911855976
role of two flagellin genes in campylobacter motility.campylobacter coli vc167 t2 has two flagellin genes, flaa and flab, which share 91.9% sequence identity. the flaa gene is transcribed from a o-28 promoter, and the flab gene from a o-54 promoter. gene replacement mutagenesis techniques were used to generate flaa+ flab and flaa flab+ mutants. both gene products are capable of assembling independently into functional filaments. a flagellar filament composed exclusively of the flaa gene product is indistinguishable in length from that of the wild t ...19911856171
enteropathogens associated with acute diarrheal disease in urban infants in são paulo, brazil.to determine the prevalence and epidemiology of enteropathogens in acute infantile diarrhea, 500 infants less than or equal to 12 months of age with diarrhea and 500 age-matched control subjects coming to a são paulo emergency room were studied. enteropathogens were identified in 55% of case infants and 10% of controls; enteropathogenic escherichia coli (epec) of classic epec serotypes producing epec adherence factor (eaf) (26% of case infants), rotavirus (14%), salmonella species (8%), enteroto ...19911856482
characterization of the helicobacter pylori urease and purification of its subunits.helicobacter pylori (formerly campylobacter pylori) is the causative agent of gastritis in man. helicobacter pylori cells contain a large amount of an extremely active urease (e.c.3.5.1.5). this enzyme is suspected to be a virulence factor since the ammonium ion produced from urea may be responsible for tissue injury and/or survival of h. pylori in the gastric environment. helicobacter pylori urease, native relative molecular mass approximately 600,000, was purified by agarose gel filtration and ...19911857197
iga nephropathy associated with campylobacter jejuni enteritis. 19911857464
interaction of cryptosporidium parvum and campylobacter jejuni in experimentally infected neonatal mice.by the method of scanning electron microscopy (sem), the inner mucosal surface of the ileum, ceacum and colon was studied in inbred balb/c mice. two-day-old mice were infected with either 10(6) oocysts of cryptosporidium parvum and 10(8) cfu of porcine and human strains of the bacterium campylobacter jejuni or with a combination of both enteropathogens. pathological changes in infection with c. parvum were related to enterocytes and villous atrophy appeared. in infection with c. jejuni, patholog ...19911863320
rates of detection of salmonella and campylobacter in meats in response to the sample size and the infection level of each species.pork, beef and chicken meat samples were collected from slaughter houses, poultry-processing plants and meat shops. rates of incidence of salmonella spp., campylobacter jejuni and c. coli with respect to the sample size were compared and the most probable number for these species were determined. salmonella spp. were detected in 69 (24.1%) of 286 chicken meat samples, in three (3.2%) of 94 pork samples, and in one (1.9%) of 52 beef samples. with chicken meat, the rates of detection were: 19.9% i ...19911863527
seroepidemiology of helicobacter pylori infection in india. comparison of developing and developed countries.helicobacter pylori (previously campylobacter pylori) is now accepted as the major cause of type b gastritis and thus what is known about the epidemiology of type b gastritis can reasonably be transferred to h. pylori. we used a specific elisa for anti-h. pylori igg to study the prevalence of h. pylori infection in a population of lower socioeconomic class from hyderabad, india. the results from india were compared to studies from other parts of the world. two hundred thirty-eight individuals ag ...19911864201
use of quinolones in the treatment of gastrointestinal infections.bacterial enteropathogens are responsible for between 40% and 80% of diarrheal illness depending upon the age of the persons affected and geographic areas where illness occurs. antibacterial agents will shorten the illness associated with enteric infection caused by enterotoxigenic escherichia coli, shigella spp. and campylobacter jejuni. these drugs also are effective in the therapy of certain clinical conditions (presumably because they are due to the same agents) which are characterized by mo ...19911864292
[oral bacterial flora and its pathogenic potential].the oral flora is a complex body formed by at least a hundred bacterial species which can be more or less regular residents in the oral cavity. thanks to a developing taxonomy related to progress made in bacterial identification, these oral species can be separated into five groups based gram staining and morphology: the gram positive cocci group (streptococcus, staphylococcus and related geni), the gram negative cocci group (neisseria, veillonella and related geni), the gram positive bacilli gr ...19911864855
high frequency of coinfecting enteropathogens in aeromonas-associated diarrhea of hospitalized peruvian infants.rectal swabs from 391 infants less than 18 months of age who were hospitalized with acute diarrhea and from 138 similarly aged healthy infants were examined for the etiologic agents of diarrhea. aeromonas spp. were recovered from 205 of 391 (52.4%) diarrheic patients, whereas they were recovered from 12 of 138 (8.7%) controls (p less than 10(-11). among the 205 aeromonas-positive diarrheic patients, 118 (57.6%) were found to be coinfected with other common enteropathogens. of the 164 aeromonas-p ...19911864933
incidence of toxigenic campylobacter strains in south africa.campylobacter strains can produce a heat-labile cytotonic toxin (cton) and various cytotoxins (ctox). of 22 south african campylobacter strains tested, 86% were toxigenic (77% produced cton, 41% produced ctox, and 32% produced both types) and 14% were toxin negative. campylobacter jejuni strains were 67% cton positive and 47% ctox positive, whereas campylobacter coli strains were 100 and 29% positive, respectively.19911864947
campylobacter neuropathy. 19911866033
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