| different pathways of uptake and degradation of sphingomyelin by lymphoblastoid cells and the potential participation of the neutral sphingomyelinase. | the metabolism of sphingomyelin (spm) was investigated in epstein-barr virus-transformed lymphoid cell lines from normal individuals and from patients with niemann-pick disease type a (deficient in the acid, lysosomal sphingomyelinase) and familial hypercholesterolemia (lacking the low density lipoprotein receptor). cells were incubated with the following radioactive or fluorescent spms: [choline-methyl-14c] spm, [oleoyl-3h]spm, pyrene-propenoyl-spm (p3:1-spm), pyrene-butanoyl-spm (p4-spm), pyre ... | 1991 | 1649823 |
| host cell phenotype-dependent methylation patterns of epstein-barr virus dna. | we have shown previously that the ebna 1 and latent membrane protein encoding regions of the epstein-barr virus (ebv) genome are highly methylated at ccgg sequences in the burkitt's lymphoma (bl)-derived cell line rael, but are unmethylated in a lymphoblastoid cell line (lcl) harbouring the same virus. to examine whether this is a regular phenomenon, we compared the methylation patterns of selected regions (bamhi c, w, h, m, e, k and n fragments) of ebv dna in representative ebv-carrying cell ty ... | 1991 | 1649897 |
| recurrent upper respiratory infection, persistent paravertebral thoracolumbar muscle spasm, and fatigue associated with chronic active epstein-barr virus infection. | | 1991 | 1650418 |
| poorly differentiated adenocarcinoma with lymphoid stroma (lymphoepithelioma-like carcinomas) of the stomach. report of three cases with epstein-barr virus genome demonstrated by the polymerase chain reaction. | lymphoepithelioma of the nasopharynx is an undifferentiated carcinoma with prominent lymphoid infiltration. histologically similar tumors have been documented in the skin, lung, thymus, salivary gland, tonsil, and uterine cervix. the authors report three cases of gastric carcinoma that were histologically and immunohistochemically similar to lymphoepithelioma. the patients were elderly white persons (61, 76, and 77 years of age); two of them had previous partial gastric resections for benign ulc ... | 1991 | 1650533 |
| epstein-barr virus in oropharyngeal and nasopharyngeal secretions of patients with nasopharyngeal carcinoma and control subjects. | the frequency of oropharyngeal excreters of the epstein-barr virus among patients with nasopharyngeal carcinoma in hong kong was compared with those of healthy adults in hong kong and california. 6 (3%) of 177 patients, 11 (12%) of 92 hong kong residents, and 20 (15%) of 132 californians were excreters. the virus was detected in the nasopharyngeal secretion of only 1 of 67 patients and in 2 of 73 healthy adults. no convincing evidence for neutralizing antibody in the throat wash and nasopharynge ... | 1991 | 1650762 |
| [hairy leukoplakia: current concepts]. | current data about oral hairy leukoplakia are reported. clinical manifestations, histological and ultrastructural features and pathogenic mechanisms are firstly described. then diagnosis are exposed. finally, management is discussed. | 1991 | 1651048 |
| epstein-barr virus transformation of cryopreserved lymphocytes: prolonged experience with technique. | | 1991 | 1651051 |
| complementation of adenovirus early region 1a and 2a mutants by epstein-barr virus immortalized lymphoblastoid cell lines. | human b-lymphocytes may be infected by both adenoviruses and the epstein-barr virus (ebv). some of the immediate early and early proteins in the two viruses are similar in function even though their primary structures are different. as these viruses might infect the same b-cells in man, we asked if complementation could take place. the adenovirus mutant h5ts125 has a thermolabile dna-binding protein and is defective in dna replication at 39 degrees. several ebv-transformed human lymphoblastoid c ... | 1991 | 1651589 |
| cooperation of ebv dna polymerase and ea-d(bmrf1) in vitro and colocalization in nuclei of infected cells. | expression of the epstein-barr virus (ebv) dna polymerase (ebvpol) open reading frame (balf5) by in vitro transcription-translation yielded a 116-kda primary translation product. enzymatic dna polymerase activity of the in vitro translated polypeptide required the presence of the 47-kda bmrf1 (ea-d) gene product. antiserum raised to the balf5 gene product expressed in escherichia coli specifically precipitated a 116-kda polypeptide in extracts of latently infected lymphoblastoid cells induced fo ... | 1991 | 1651595 |
| phospholipase a2 activity in epstein-barr virus-transformed lymphoblast cells from schizophrenic patients. | we examined the activity of phospholipase a2 in epstein-barr virus-transformed lymphoblast cell lines established from ten schizophrenic patients and ten controls. a novel method for determination of enzyme activity in whole cells was employed, by measuring the hydrolysis of a fluorescent analogue of phosphatidylcholine. no significant difference in phospholipase a2 activity was found between the groups. these results suggest that the previously reported changes in phospholipase a2 activity in p ... | 1991 | 1651772 |
| epstein-barr virus antibody patterns preceding the diagnosis of nasopharyngeal carcinoma. | nasopharyngeal carcinoma (npc) patients have elevated igg and iga antibody titers against the epstein-barr viral capsid antigen (vca) and the diffuse component of the early antigen complex (ea-d) at diagnosis. several studies have implied that the presence of anti-vca-iga can be used as a screening marker for early npc. to evaluate this further, we undertook a serologic case-control study based on four serum banks which together had specimens from over 240,000 persons. seven cases of undifferent ... | 1991 | 1651778 |
| evidence for molecular subtypes of hiv-associated lymphoma: division into peripheral monoclonal, polyclonal and central nervous system lymphoma. | the pathogenesis of the hiv-associated lymphomas is not well understood. in order to begin characterizing this class of lymphoma, we initiated a molecular genetic study of dna extracted from 31 diagnostic biopsy specimens from patients diagnosed with aids-associated non-hodgkin's lymphoma. analysis of 25 peripheral lymphomas showed that 14 were monoclonal b-cell processes, while 11 appeared to be of polyclonal origin. five of the 14 monoclonal lymphomas were found to have rearrangements of the c ... | 1991 | 1652978 |
| the latent membrane protein-1 in epstein-barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre. | immunofluorescence studies on epstein-barr virus (ebv)-transformed lymphoblastoid cells have previously shown that the latent membrane transforming protein (lmp-1) is found in patch-like inclusions which also immunostain for vimentin. we now show that ebv transformation causes a major reorganization of intermediate filaments, microtubules, mitochondria, and lysosomal elements, which generally become oriented around the microtubule organizing centre. immunogold electron microscopy shows that lmp- ... | 1991 | 1653831 |
| [epstein-barr virus (ebv) in lymphoproliferative diseases]. | the results of dna or rna study in ebv-associated lymphoproliferative diseases were shown. for detecting ebv dna, southern blot analysis with bam hiw probe (internal repeat) and non-repeat (least often deleted) probe are used. probes close to (ex. lmp) or within terminal repeat can indicate clonality in terms of junctional structure. several such examples were shown, in which benign polyclonal ebv (+) cd3 + 8+ lymphocytes, ebv (+) cd3 + 4 - 8- granulay lymphocytosis, ebv (+) t(14, 22) b-cell lym ... | 1991 | 1653862 |
| human herpesvirus-6 infection may predispose cells to superinfection by other viruses. | double infection of cells by hhv-6, epstein-barr virus (ebv) and by human immunodeficiency virus (hiv-1) can enhance viral effects though genetic transactivation. it remained to be clarified, however, by which mechanism different viruses may enter the same cell. we have shown that hhv-6 infection of immature lymphoid cells rigidifies the cytoplasmic membrane and causes receptor proteins for viruses such as cd4 for hiv-1 and cr2 for ebv to be expressed. in our experiments, hhv-6 infected cells we ... | 1991 | 1654148 |
| identification of the human herpesvirus 6 glycoprotein h and putative large tegument protein genes. | determination of the nucleotide sequences of two molecular clones of human herpesvirus 6 (hhv-6) (strain gs) and comparison with those of human cytomegalovirus (hcmv) has allowed the identification of the genes for the glycoprotein h (gh) and the putative large tegument protein of hhv-6. two molecular clones of fragments of hhv-6, the bamhi-g fragment (7,981 bp) of the clone termed pzvb43 and a hindiii fragment (8,717 bp) of the clone termed pzvh14, represent approximately 10% of the hhv-6 genom ... | 1991 | 1654455 |
| a pathologic study of childhood lymphoma in taiwan with special reference to peripheral t-cell lymphoma and the association with epstein-barr viral infection. | the authors retrospectively reviewed the clinicopathologic and immunologic features of 65 consecutive cases of childhood lymphoma reported between 1980 and 1989. southern blot hybridization was also performed in 23 cases to study their association with epstein-barr virus (ebv) and human t-cell leukemia virus type 1 (htlv-1). the 65 cases included 56 non-hodgkin's lymphoma (nhl) (86%) and 9 hodgkin's disease (hd) (14%). the nhl could be classified into the following groups: group i, small nonclea ... | 1991 | 1655230 |
| production of islet cell antibodies from epstein-barr virus-transformed peripheral blood lymphocytes in type 1 (insulin-dependent) diabetic patients. | islet cell antibodies are usually detected in the sera of almost all type 1 (insulin-dependent) diabetic patients within several months after onset of the disease. the antibodies then disappeared quite early during the course of the disease. the present study was undertaken to detect islet cell antibody-producing clones in peripheral blood lymphocytes of type 1 diabetic patients whose islet cell antibodies could not be detected in sera. epstein-barr virus-transformed lymphocytes were employed to ... | 1991 | 1655542 |
| transcriptional activity across the epstein-barr virus genome in raji cells during latency and after induction of an abortive lytic cycle. | we have studied the relative rate of transcription across the epstein-barr virus genome in the burkitt's lymphoma cell line raji by nuclear run-on analysis during latency and after induction of an abortive lytic cycle with 12-0-tetradecanoylphorbol 13-acetate (tpa) and 5-iodo-2'-deoxyuridine (iudr). during latency the entire, or almost the entire, viral genome was found to be transcriptionally active to a low or intermediate extent, with some variation in activity along the genome. the fragment ... | 1991 | 1655954 |
| clinical and serological studies in a series of 45 patients with guillain-barré syndrome. | we retrospectively reviewed the clinical files of 45 guillain-barré syndrome (gbs) patients admitted to our department between 1979 and 1989. the age distribution was bimodal with a first peak in young adults (20-40 years), and a second one between 60 to 70 years. seasonal distribution showed a late fall and a hivernal predominance. three patients experienced a second attack of gbs 2-9 years after the first one. thirty-one (69%) presented antecedent events, most often a respiratory tract infecti ... | 1991 | 1655983 |
| acquired von willebrand disease after epstein-barr virus infection. | | 1991 | 1656006 |
| spousal caregivers of dementia victims: longitudinal changes in immunity and health. | providing long-term care for a demented relative profoundly affects caregivers' lives. we assessed changes in depression, immune function, and health in 69 spousal caregivers who had already been caregiving for an average of five years and 69 sociodemographically matched control subjects. between the initial sample ("intake") and the follow-up data collected an average of 13 months later, caregivers showed decrements relative to controls on three measures of cellular immunity. caregivers also re ... | 1991 | 1656478 |
| from host components of virus particles to anti-myelin human monoclonal antibodies obtained by epstein-barr virus transformation of lymphocytes from multiple sclerosis patients. | human monoclonal antibodies against viruses and host components were obtained after transformation of human b cells by epstein-barr virus, their cloning and propagation. b cells were mainly obtained from peripheral blood of healthy individuals or patients with neurological diseases, especially with multiple sclerosis (ms). the obtained autoantibodies reacted with cell components, i.e. with the nucleus, cytoplasm or cytoskeleton. using myelin in the screening procedure, about 30 monoclonal antibo ... | 1991 | 1656931 |
| non-hodgkin's lymphomas in patients with human immunodeficiency virus infection. presence of epstein-barr virus by in situ hybridization, clinical presentation, and follow-up. | the authors studied all patients with serologic evidence of human immunodeficiency virus (hiv) infection and malignant non-hodgkin's lymphoma (nhl) that presented at a single hospital from 1982 to 1989. sixteen patients were identified, all white homosexual men with a mean age of 38.2 years. lymphoma was the initial presentation of hiv infection in 37.5%. sixty-two percent of the cases had a high-grade nhl, 31% had intermediate-grade, and 6% (one patient) had a low-grade lymphoplasmacytoid lymph ... | 1991 | 1657357 |
| the glycoproteins of the human herpesviruses. | the herpesvirus family contains several important human pathogens. human herpesviruses include herpes simplex virus type 1 and 2, varicella-zoster virus, human cytomegalovirus, epstein-barr virus and human t-cell lymphotropic virus. the general property of herpesviruses is their ability to establish latency and to be periodically reactivated. all human herpesviruses contain a subset of genes encoding viral glycoproteins that are clearly homologous, and their similarity is significantly greater a ... | 1991 | 1657514 |
| human t-cell recognition of epstein-barr virus-induced replication antigen complexes. | cellular immune responses to epstein-barr virus (ebv)-associated antigens play an important role in the control of ebv-immortalized b lymphocytes. the nature of the antigens that serve as targets for these responses remains largely unknown. the purpose of the experiments reported here was to determine if virus-replication-associated antigens might function as targets for t-cell immunity with the emphasis on ebv-induced early antigen (ea) complex. activated t-cell populations directed against thi ... | 1991 | 1657795 |
| a comparison of epstein-barr virus specific proteins expressed by three epstein-barr virus isolates using specific monoclonal antibodies. | monoclonal antibodies (mabs) were prepared in mice inoculated with a nasopharyngeal carcinoma epstein-barr virus (npc-ebv) producer lymphoblastoid cell line (npc-lc). when the 214a9 mab was used to study the expression of the 52/50-kd early antigen in npc-lc, b95-8, and hr-1 cells, no difference was found. the same results were obtained with the 343d12 mab which is to a 125-kd virus capsid antigen protein. however, the 244g11 mab detected an ebv membrane antigen (ma) with a molecular weight of 2 ... | 1991 | 1657827 |
| delineation of the cis-acting element mediating ebna-2 transactivation of latent infection membrane protein expression. | to delineate the cis-acting element through which ebna-2 transactivates latent membrane protein 1 (lmp1), we assayed the effect of ebna-2 on the activity of lmp1 promoter upstream deletion mutants in the context of the lmp1 or heterologous promoters controlling chloramphenicol acetyltransferase (cat) reporter gene expression in epstein-barr virus-negative burkitt lymphoma cells. assays of progressive 5' deletions of the lmp1 promoter revealed low constitutive and at least eightfold ebna-2-stimul ... | 1991 | 1658373 |
| the epstein-barr virus nuclear protein encoded by the leader of the ebna rnas is important in b-lymphocyte transformation. | these experiments evaluate the role of the epstein-barr virus (ebv) nuclear antigen leader protein (ebna-lp) in b-lymphocyte growth transformation by using a recombinant ebv molecular genetic approach. recombinant viruses encoding for a mutant ebna-lp lacking the carboxy-terminal 45 amino acids were markedly impaired in their ability to transform primary b lymphocytes compared with ebna-lp wild-type but otherwise isogenic recombinant viruses. this impairment was particularly evident when primary ... | 1991 | 1658376 |
| in vivo effects of sera from guillain-barré subgroups: an electrophysiological and histological study on rat nerves. | serum from 20 patients with guillain-barré syndrome (gbs), 10 healthy controls and 10 patients with recent cytomegalovirus, epstein-barr virus, or campylobacter jejuni/coli infections was injected into rat sciatic nerve. the 20 gbs patients consisted of 2 groups of 10 patients with different electrophysiological and clinical disease patterns. the main aim of the study was to investigate possible differences in humoral (auto)-immunity between these subgroups. we found no statistically significant ... | 1991 | 1658645 |
| epstein-barr virus-associated lymphoproliferative disorders in immunocompromised individuals. | | 1991 | 1659123 |
| conjunctival lymphocytic nodule associated with the epstein-barr virus. | infection with epstein-barr virus has been reported to have numerous systemic and ocular manifestations. in this study, a 38-year-old man with acute infectious mononucleosis was examined for a painless left red eye of three days' duration. the patient had a two-week history of fatigue, low-grade fever, sore throat, and lymphadenopathy. serologic evaluation was indicative of an acute primary infection with epstein-barr virus. a large, salmon-colored, supranasal bulbar conjunctival mass was observ ... | 1991 | 1659201 |
| epstein-barr virus: an aetiological factor in cutaneous lymphoproliferative disorders? | | 1991 | 1659624 |
| [studies on human porin. v. the expression of "porin31hl" in the plasmalemma is not by cell transformation]. | in recent papers we proved "porin 31hl" to be located on the surface of human, ebv-transformed b lymphocytes. here we present proof of "porin 31hl" in the plasmalemma of normal human blood lymphocytes. for this purpose b and t lymphocytes were isolated from human heparinized blood and examined by indirect immunofluorescence techniques using different monoclonal antibodies against purified "porin 31hl" and some b and t cell markers, respectively. for comparison a number of established cell lines ... | 1991 | 1659831 |
| distribution and engraftment patterns of human tonsillar mononuclear cells and immunoglobulin-secreting cells in mice with severe combined immunodeficiency: role of the epstein-barr virus. | tonsils seem to be the ideal source of lymphocytes seeding to the mucosa of the respiratory tract. the distribution and engraftment of human lymphocytes injected into mice with severe combined immunodeficiency (scid) are not well understood. c.b-17 scid mice were injected intraperitoneally with human tonsillar mononuclear cells (hu-tmc). the hu-tmc-scid mouse chimeras were subsequently tested for the appearance and distribution of human lymphocytes tagged with h33342 and immunoglobulin-secreting ... | 1991 | 1660036 |
| is there a unique episome in ebv transformed b cells? | we have examined the frequency of episome formation in resting b cells 36 hr after infection with epstein-barr virus. we have detected an average of 0.65 episomal genomes per cell (sd 0.17). we have used this information to predict the distribution of circularization events in b cell clones derived from such infections. we have observed that in 32 out of 33 cases (with one unresolved) the b cell clone has arisen as a consequence of a single circularization event. this result is highly statistica ... | 1991 | 1660206 |
| early events in epstein-barr virus genome expression after activation: regulation by second messengers of b cell activation. | rna transcription from the bamhi z and bamhi r and hindiii g regions of the epstein-barr virus (ebv) genome was studied after treatment of akata cells with anti-immunoglobulin g (igg), with second messenger agonists or antagonists to determine how latent ebv activation is regulated by b cell second messengers. northern gel analysis demonstrated that bzlf1, bzlf1 + brlf1, and bmlf1 + bslf2 transcripts were induced at 2 hr and increased in concentration at 4 hr after induction with anti-igg; trans ... | 1991 | 1660209 |
| [importance and diagnosis of epstein-barr virus infection]. | the 103 serum samples have been examined for the presence of the specific antibodies against epstein-barr virus (ebv): igg and igm antibodies to viral capsid antigen (vca), antibodies to nuclear antigen (ebna) and antibodies to early antigen (ea). sixty nine studied sera belonged to patients with suspected infective mononucleosis (im) while other sera were sent with the diagnosis which could be associated with ebv infections. the results obtained have suggested the necessity of examinations of e ... | 1991 | 1660212 |
| recurrent mucocutaneous lymphocytic infiltration possibly associated with epstein-barr virus infection. | a 32-year-old man had a history of recurrent but self-healing necrotizing nodules on the face and oral mucosa. histologically the lesion was composed of necrotizing foci surrounded with dense cd8+ t lymphocytic infiltration in the dermis. laboratory examination showed marked blood cd8+ t lymphocytosis with a reversed ratio of cd4+/cd8+ of 0.11. except for a very high titer of antibodies against epstein-barr-virus-associated antigens, no signs of infection were observed. | 1991 | 1660415 |
| quantitation of t cell depletion by limiting dilution analysis. | we evaluated a culture method for enumeration of residual t cells remaining in marrow after treatment with antibody and complement or with immunotoxin. | 1991 | 1660438 |
| the x-linked lymphoproliferative disease: from autopsy toward cloning the gene 1975-1990. | although x-linked lymphoproliferative disease (xlp) is rare (1-2 males per 1 x 10(6)), it serves as a model for discerning diverse diseases caused by epstein-barr virus (ebv) ranging from agammaglobulinemia to fatal infectious mononucleosis following infection with the virus. the study of patients with xlp has also paved the way to understanding how ebv induce diseases in children with primary immunodeficiency diseases, organ transplant recipients, and those with acquired immunodeficiency syndro ... | 1991 | 1660601 |
| induction of nad(p)h:quinone reductase in human peripheral blood lymphocytes. | the induction of quinone reductase [qr; nad(p)h:(quinone acceptor) oxidoreductase; ec 1.6.99.2] in cultured cells and animal tissues of rodents has provided useful information on mechanisms of protection against carcinogens. we have developed a simple and efficient microtiter plate assay for the direct measurement of qr basal activity and inducibility in human peripheral blood lymphocytes (unstimulated, mitogen-stimulated and epstein-barr virus-transformed) grown in suspension culture. in these ... | 1991 | 1660793 |
| epstein-barr virus infections and hodgkin's disease: a study of fixed tissues using the polymerase chain reaction. | epstein-barr viral (ebv) infections are associated with hodgkin's disease (hd). to better characterize this relationship, fixed tissues of infectious mononucleosis, normal and reactive lymph nodes, lymph nodes with progressively transformed germinal centers, and biopsy specimens with the different subtypes of hd were analyzed by polymerase chain reaction (pcr). the presence or absence of ebv, the relative amounts of ebv, and the presence of multiple ebv genotypes as defined by amplification of a ... | 1991 | 1660852 |
| a t cell clone in association with an epstein-barr virus-related b cell lymphoma. | | 1991 | 1661038 |
| pathology of malignant lymphomas. | attempts to understand the malignant lymphomas have followed two major lines of investigation: attempts to define the relationship of neoplastic lymphoid cells to stages of normal t- and b-lymphocyte differentiation, and attempts to understand the genetic abnormalities associated with malignant transformation. it is hoped that these two lines of investigation will make it possible to define clinically important entities, so that specific treatment strategies can be developed and tested, and will ... | 1991 | 1661168 |
| interleukin 10. | | 1991 | 1661188 |
| frequent detection of epstein-barr viral deoxyribonucleic acid and absence of cytomegalovirus deoxyribonucleic acid in hodgkin's disease and acquired immunodeficiency syndrome-related hodgkin's disease. | epstein-barr viral dna (ebv dna) has been detected in 20 to 58% of hodgkin's disease tumors analyzed by southern blotting or polymerase chain reaction (pcr). because patients with hodgkin's disease are generally immunodepressed, it is possible that the ebv is not directly involved in the pathogenesis of hodgkin's disease but is merely detectable by molecular techniques because of reactivation of a latent infection. the purpose of this study was to determine if ebv dna could be detected in an eve ... | 1991 | 1661351 |
| [the etiology of malignant lymphomas]. | | 1991 | 1661666 |
| characterization of rhesus macaque b-lymphoblastoid cell lines infected with simian type d retrovirus. | a simian type d retrovirus designated srv induces a fatal immunosuppressive disease in rhesus macaques. this syndrome shows many clinical similarities to acquired immunodeficiency syndrome (aids) in human immunodeficiency virus-infected individuals. to investigate the mechanisms of immune dysfunction in srv infection, we have focused on the interactions of srv serotype 1 (srv-1) with macaque b-lymphoblastoid cell lines (b-lcl). procedures were optimized for establishing b-lcl by immortalization ... | 1991 | 1662056 |
| cutaneous lesions of hemophagocytic syndrome in a patient with t-cell lymphoma and active epstein-barr infection. | the hemophagocytic syndrome (hps) was first described in immune-compromised patients with secondary viral infections. immunodeficiency has not always been diagnosed before the onset of hps, but most patients who develop hps have known immune deficits. hps has also been reported in association with lymphoreticular malignancies. in hps the most prominent histologic change is phagocytosis of red blood cells and other bone marrow-derived elements by cytologically benign histiocytes. considerable con ... | 1991 | 1662240 |
| genotypic and phenotypic evidence of t-cell leukemia in a patient successfully treated by interferon-alpha for typical hairy cell leukemia. | a 53-year-old man was diagnosed to have typical hairy cell leukemia. immunophenotyping of frozen splenic tissue showed clonality of hairy cells for mu lambda, confirmed by the corresponding immunoglobulin gene rearrangements. the patient was successfully treated with interferon-alpha (if-alpha). in the fifth year of treatment with if-alpha the morphology of peripheral blood mononuclear cells (pbmc) and of bone marrow infiltration changed with the appearance of numerous small to intermediate shap ... | 1991 | 1663564 |
| sezary cell lymphoma following cyclosporin immunosuppression for renal transplantation. | | 1991 | 1663600 |
| a human anti-jkb monoclonal antibody. | peripheral blood mononuclear cells of an immunized patient were transformed with epstein-barr virus and then fused with p3x63ag8 mouse myeloma cells by polyethylene glycol. after the cloning, a hybridoma cell line secreting specific anti-jkb monoclonal antibody was isolated. the antibody was produced in supernatant form and tested for its use as a blood grouping reagent. | 1991 | 1663674 |
| maple syrup urine disease: clinical and biochemical significance of gene analysis. | | 1991 | 1664011 |
| adenosine deaminase activity in relation to the appearance of early and late epstein-barr virus antigens induced in lymphoblastoid cells. | induction of epstein-barr virus (ebv) capsid antigen synthesis in 59.6% of p3hr-1 cells was followed by a decrease to 70% in adenosine deaminase (ada) activity. in daudi cells synthesizing ebv early antigen, ada activity did not decrease. | 1991 | 1664042 |
| evolving principles in immunopathology: interleukin 10 and its relationship to epstein-barr virus protein bcrf1. | | 1991 | 1664983 |
| x-linked lymphoproliferative disease (xlp) as a model of epstein-barr virus-induced immunopathology. | | 1991 | 1664985 |
| relationship between antibody production to epstein-barr virus (ebv) early antigens and various ebv-related diseases. | | 1991 | 1664988 |
| regulation of immune activation/retroviral replication by cd8+ t cells. | | 1991 | 1665323 |
| peptide competition at the level of mhc-binding sites using t cell clones from a rheumatoid arthritis patient. | the inhibition of antigen presentation in rheumatoid arthritis by blocking peptide binding to mhc at the antigen presenting cell (apc) level was investigated using various synthetic peptides derived from the 65 kda mycobacterial protein. human t cell clones from tuberculosis and rheumatoid arthritis patients were stimulated with peptides in the presence of irradiated apcs (autologous or dr homozygous ebv-b cell lines). two peptides (residues 65-85 and 412-426) were found to be able to bind to th ... | 1991 | 1665695 |
| transient lupus anticoagulant induced by epstein-barr virus infection. | a 25-year-old woman presented with an episode of left calf deep vein thrombosis and pulmonary thrombosis. she was found to have a lupus anticoagulant with anticardiolipin antibodies, some autoimmune antibodies and antibodies for primary epstein-barr (eb) virus infection. six months later, lupus anticoagulant and other autoimmune antibodies were found to be negative and eb virus antibodies were shown to be seroconverted. we suggest that the transient presence of lupus anticoagulant was due to eb ... | 1991 | 1665993 |
| ubiquitin and the lysosome system: molecular immunopathology reveals the connection. | considerable advances in our understanding of the molecular pathology of the major human neurodegenerative diseases have been made by the use of ubiquitin immunocytochemistry. the technique demonstrates that filamentous inclusions and vacuoles contain ubiquitin-protein conjugates. the molecular structure of the filaments and the morphological type of vacuoles is not completely understood but there is evidence that some of the filamentous inclusions contain intermediate filaments and the perinucl ... | 1991 | 1666279 |
| oral manifestations of viral infections in immunocompromised patients. | viral infections are a significant cause of morbidity and mortality in immunosuppressed patients. it is clear that diseases or medical treatments that have cytostatic or cytotoxic effects on lymphocytes and disrupt cytokine production or activity increase the risk of viral infections. while the rate of viral infection varies with the nature and degree of immunosuppression, it is clear that reactivation of latent virus is the most important determinant of the types of viral infections most freque ... | 1991 | 1666308 |
| activation and fixation of c3 by human b cell lines is enhanced by interferon-gamma and tumor necrosis factor alpha. | human b cell lines were tested for the capacity to convert c3 to c3b/ic3b through the alternative c pathway (acp) and to fix the generated c3 fragments. the panel of lines included four epstein-barr virus (ebv)-negative burkitt's lymphomas and their sublines converted with two ebv strains: the prototype b958 (b virus) and the nontransforming p3hr-1 (p virus). comparison of these groups of parallel lines (derived from a single patient) showed that the ebv-carrying cells were more efficient in act ... | 1991 | 1666351 |
| vaccination against herpesvirus, fiction or reality? | against herpes simplex type 1 (hsv-1) there are commercially available vaccines which may induce protection against symptoms of primary infections, but not against establishment of latency. effective animal vaccines have been developed against herpes simplex-like viruses, for example pseudorabies vaccine for pigs, and an experimental vaccine can protect cattle against infection with infectious bovine rhinotracheitis (ibr) virus. there are also commercially available effective live vaccines again ... | 1991 | 1666442 |
| inhibitory effects of acyclic nucleoside phosphonate analogs, including (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine, on epstein-barr virus replication. | (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine [(s)-hpmpa], (s)-9-(3-hydroxy-2-phosphonylmethoxypropyl)-2,6-diaminopurine [(s)-hpmpdap], cyclic (s)-hpmpa [(s)-chpmpa], 9-(2-phosphonylmethoxyethoxyethyl)-2,6-diaminopurine (pmedap), and (s)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine [(s)-hpmpc] were examined for their inhibitory effects on epstein-barr virus (ebv) replication. the 50% effective concentrations for inhibition of viral dna replication were 0.16, 0.03, 2.0, 1.5, and 0.08 micr ... | 1991 | 1666500 |
| [clinical and pathogenetic observations on children with chronic mononucleosis]. | epstein-barr virus is seldom the causative agent of a prolonged atypical illness, known as chronic mononucleosis syndrome, characterized by a persistent pattern of clinical manifestations and by a defective immune response to specific viral antigens. this paper refers about 6 children for whom clinical and serological findings suggest the chronic epstein-barr virus infection. the authors believe that this chronic state might be explained by the unusual antibody pattern to ebv virus, with the per ... | 1991 | 1664943 |
| production of anti-tumor-promoting iridoid glucosides in genipa americana and its cell cultures. | the genipa americana plant contains geniposide [3] and geniposidic acid [2] in the fruits and geniposidic acid [2] in the leaves. on callus induction, the plant produces tarennoside [1], geniposidic acid [2], and gardenoside [4] in high levels. the leaves of ge. americana plants redifferentiated from the callus tissues produce 2. | 1991 | 1667413 |
| development and evaluation of a capture elisa for igm antibody to the human cytomegalovirus major dna binding protein. | a new capture elisa (elab) for determination of the igm antibody response to the human cytomegalovirus major dna binding protein (p52) was developed by using a p52-specific monoclonal antibody. as a reference test, a capture elisa using in parallel viral- and cell-control labeled antigens (ela) was employed. general specificity, which was determined on 180 unselected igm-negative sera from an adult population was 100%; stringent specificity, which was evaluated on 108 potentially interfering ser ... | 1991 | 1667791 |
| aetiology and pathogenesis of non-hodgkin lymphoma in aids. | the aids-nhls constitute a histologically diverse group of tumours. comparisons with some groups of non-aid-nhls, including ebl, sbl and large cell lymphoma, have been made and similar pathogenetic mechanisms postulated for such groups. however, the incidence of the different pathological types of nhl in aids is not yet clear, and further work is necessary to determine the contribution of factors such as c-myc translocations and the presence of ebv. aids-nhls are clearly a more diverse group of ... | 1991 | 1668231 |
| [hepatitis by infectious mononucleosis]. | five adult patients presenting with clinical and laboratory manifestations of an acute hepatitis in the course of a hitherto undiagnosed infectious mononucleosis (im) are reviewed. chief complaints were intense malaise and prolonged fever (7 to 15 days prior to diagnosis). serum aminotransferases were moderately raised in all patients; three patients had mild jaundice with a direct-reacting hyperbilirubinemia; 4 patients had an enlarged and tender liver. when making the differential diagnosis of ... | 1991 | 1668992 |
| search for an association between epstein-barr virus infection and the chronic fatigue syndrome. | | 1991 | 1669352 |
| virology laboratory diagnosis of chronic fatigue syndrome. | | 1991 | 1669355 |
| epstein-barr virus transmission via the donor organs in solid organ transplantation: polymerase chain reaction and restriction fragment length polymorphism analysis of ir2, ir3, and ir4. | two organ transplant recipients who received organs from a common donor and were diagnosed as having an epstein-barr virus (ebv)-associated posttransplant lymphoproliferative disorder were studied to determine the mode of ebv transmission. the results of restriction fragment length polymorphism, polymerase chain reaction, and minisatellite dna analyses demonstrate that both patients had a common strain of ebv and that this strain was transmitted from the donor's organs to both recipients. posttr ... | 1991 | 1670959 |
| diagnosis of nasopharyngeal carcinoma by means of recombinant epstein-barr virus proteins. | the immune response of patients with nasopharyngeal carcinoma to epstein-barr virus (ebv) antigens is diagnostic of the tumour. existing tests use ebv antigens produced in ebv-infected lymphoblastoid cells, but the virus replicates poorly in these cells. serum samples from 18 patients diagnosed as having nasopharyngeal carcinoma were screened by western blot analysis, enzyme-linked immunosorbent assay (elisa), and immunofluorescence tests for antibodies to the ebv-coded alkaline deoxyribonucleas ... | 1991 | 1672175 |
| circulating epstein-barr virus-carrying b cells in acute malaria. | epstein-barr virus (ebv) infection and plasmodium falciparum malaria are two known cofactors in the aetiology of endemic burkitt's lymphoma. to assess the relation between these factors, limiting dilution analysis was used to assess the number of ebv-carrying b cells in the circulation of gambian children during and after acute malaria. numbers of virus-carrying cells were five times higher in acute malaria patients and in uk patients with infectious mononucleosis than in convalescent malaria pa ... | 1991 | 1672968 |
| binding of epstein-barr virus small rna eber-1 to the double-stranded rna-activated protein kinase dai. | epstein-barr virus encodes two small rnas, eber-1 and -2, that are abundantly expressed in latently infected cells. recent evidence suggests a role for eber-1 in regulation of translation since this rna is able to prevent the inhibition of protein synthesis by double-stranded rna in rabbit reticulocyte lysates. we show here that eber-1 that has been synthesized in vitro forms a complex with the dsrna-activated inhibitor of protein synthesis dai, a protein kinase that specifically phosphorylates ... | 1991 | 1673026 |
| viral and cellular gene expression in cd4+ human lymphoid cell lines infected by the simian immunodeficiency virus, siv/mne. | our laboratory has undertaken an analysis of cellular and viral gene expression in cd4+ human lymphoid cell lines infected by the human and simian immunodeficiency viruses, hiv-1 and siv/mne, respectively. the purpose of the current study was to: (i) examine the effects of siv/mne infection on host macromolecular synthesis and compare the results to those in the hiv-1 system; and (ii) investigate the mechanisms responsible for the restriction of siv/mne infection in cd4 positive lymphoid cells w ... | 1991 | 1675822 |
| characterization of icam-2 and evidence for a third counter-receptor for lfa-1. | in an endeavor to further characterize human intercellular adhesion molecule-2 (icam-2), two murine monoclonal antibodies (mab) were generated to icam-2 transfected cos cells, and designated cbr-ic2/1 and cbr-ic2/2. immunoprecipitated, reduced icam-2 migrated as a broad band of mr 60,000 in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. treatment with n-glycanase revealed a peptide backbone of mr 31,000, consistent with the size predicted from the cdna. icam-2 had a broad distributio ... | 1991 | 1676048 |
| histiocytic medullary reticulosis, a lethal form of epstein-barr-virus-related disorder. | | 1991 | 1678075 |
| epstein-barr virus transactivator antibodies in hiv infection. | | 1991 | 1678828 |
| the epstein-barr virus bnlf-1 membrane protein lmp1 induces homotypic adhesion mediated by cd11a/cd18 in a murine b-cell line, mimicking the action of phorbol ester. | expression of the epstein-barr virus bnlf1 gene (lmp1) or treatment with 4b-phorbol-12,13-dibutyrate in the murine pro-b-cell line a/j-95 leads to a five- to sevenfold enhancement of homotypic adhesion without significant changes in adhesion molecule expression. antibody to cd11a inhibits aggregation, indicating that cd11a/cd18-mediated adhesion is a common target for both agents. | 1991 | 1680199 |
| lack of bcr rearrangement in juvenile chronic myeloid leukemia. | juvenile chronic myeloid leukemia (jcml) is an unusual subtype of children's leukemia, characterized by unique clinical presentation. recent studies revealed several biological features, distinguishing from those observed in adult type chronic myeloid leukemia (acml). the majority of acml cases are characterized by the presence of an hybrid bcr-abl rearranged gene. in an effort to elucidate the molecular basis of this unusual leukemia we looked for bcr rearrangement in six jcml cases. no bcr rea ... | 1991 | 1683680 |
| cross-reactivity between the ebna-1 p107 peptide, collagen, and keratin: implications for the pathogenesis of rheumatoid arthritis. | an unusually heavy load of epstein-barr virus (ebv) infection and autoimmunity to collagen are believed to be contributing factors to the pathogenesis of rheumatoid arthritis (ra). the present report presents data showing that p107, the major epitope of the ebv-encoded ebna-1 antigen, cross-reacts with denatured collagen (dc) and keratin (k), suggesting a new likely link among ra, ebv-1, and these autoantigens. a radioimmunoassay using antigen-coated microtiter plates was used to demonstrate ant ... | 1990 | 1688406 |
| an epstein-barr virus-specific cytotoxic t cell epitope in ebv nuclear antigen 3 (ebna 3). | epstein-barr virus (ebv)-specific ctl clones were isolated that recognized a-type ebv transformants but not b-type transformants. these a-type-specific ctl clones (hla b8 restricted) were used to screen peptides derived from the ebv nuclear antigens (ebnas) 2, 3, 4, and 6 as potential ctl epitopes. of the 76 peptides screened, one sequence from ebna 3 (residues 329-353) was recognized by a-type-specific ctl clones after absorption onto target cells (either autologous b-type transformants or pha ... | 1990 | 1688611 |
| cd5 and immunoglobulin v gene expression in b-cell lymphomas and chronic lymphocytic leukemia. | we studied the expression of cd5 and immunoglobulin variable gene families in a panel of monoclonal epstein-barr virus (ebv) transformed lines, chronic lymphocytic leukemias (clls) and cd5+ and cd5- b-cell lymphomas. the cd5 gene expression was in all cases identical to that of t-cell malignancies. the utilization of the various vh and vk gene families was roughly proportional to the estimated gene family size in ebv lines obtained from adult healthy subjects. in contrast we found a statisticall ... | 1990 | 1690580 |
| patterns of reactivity of epstein-barr virus-specific t cells in a-type donor cultures after reactivation with autologous a- or b-type transformants. | regression endpoints were assessed in cultures from 11 epstein-barr (eb) virus a-type seropositive donors and 2 seronegative donors using a- and b-type eb virus preparations. in 9/11 of the seropositive donors, the resulting endpoints using a-type or b-type virus were similar and demonstrated a significant t cell response to both virus types. however, the regression endpoints for 2/11 seropositive donors were reproducibly higher with b-type virus compared with a-type virus, indicating a weak t c ... | 1990 | 1690609 |
| cross-recognition of a mouse h-2-peptide complex by human hla-restricted cytotoxic t cells. | epstein-barr virus (ebv)-specific human cytotoxic t lymphocyte (ctl) lines from a virus-immune hla-a11+ donor cmc produced significant lysis of transfected mouse p815 target cells (h-2d background) expressing an introduced hla-a11 heavy chain gene and the ebv gene encoding latent membrane protein (lmp). to identify the target epitope being recognized, hla-a11+ transfectants of p815/a11) were pre-exposed to synthetic peptides corresponding to fragments of the lmp sequence and the cells then teste ... | 1990 | 1690661 |
| disease-associated human histocompatibility leukocyte antigen determinants in patients with seropositive rheumatoid arthritis. functional role in antigen-specific and allogeneic t cell recognition. | the susceptibility to develop seropositive rheumatoid arthritis (ra) has been linked to specific genomic polymorphisms within the hla complex. two different haplotypes have been associated with the disease, hla-dr1 and hla-dr4. to investigate the link between such phenotypic disease associations and potential immune mechanisms we used alloreactive and antigen-specific human t cell clones. here we describe a panel of alloreactive t cell clones directed to polymorphic determinants encoded by the t ... | 1990 | 1690755 |
| production and characterization of a human monoclonal antibody, reactive with a conserved epitope on gp41 of human immunodeficiency virus type i. | a human epstein-barr virus-transformed lymphoblastoid b-cell line was generated from peripheral blood mononuclear cells (pbmc) of an asymptomatic human immunodeficiency virus type i (hiv-1) seropositive donor, which produces a human monoclonal antibody k14 (igg1), reactive with an epitope on the transmembrane part (gp41) of the envelope glycoprotein of hiv-1. this monoclonal antibody reacts with a lysate of hiv-1-infected h9 cells, gradient purified hiv-1, and a vaccinia recombinant hiv-1 gp160 ... | 1990 | 1692724 |
| morphological transformation of human keratinocytes expressing the lmp gene of epstein-barr virus. | the association of epstein-barr virus (ebv) with nasopharyngeal carcinoma (npc) has been known for some time, but the precise role of ebv in this cancer is poorly understood, due partly to the lack of an in vitro system for studying npc cells and the effect of ebv on epithelial cells. biopsies of npc tumours have revealed expression of the ebv latent membrane protein (lmp) in 65% of cases, suggesting that in at least some npc tumours lmp may contribute to cell transformation. here we address the ... | 1990 | 1692971 |
| a cell-to-cell mediated interaction involving monocytes and non-t/cd16+ natural killer (nk) cells is required for histamine h2-receptor-mediated nk-cell activation. | histamine (10(-4) to 10(-7) m) augmented natural killer cell cytotoxicity (nkcc) of human cd16+, non-t lymphocytes in vitro against the nk-sensitive target cells k562 erythroleukaemic, molt-4 lymphoma, chang liver cells and against epstein-barr virus (ebv)-transformed, nk-insensitive daudi b-lymphoblastoid target cells by a mechanism of action involving a prostaglandin- and interleukin 1 (il-1)-independent accessory function of monocytes. no evidence for the production of intermediary, nk-enhanc ... | 1990 | 1693229 |
| serum and salivary iga antibodies against a defined epitope of the epstein-barr virus nuclear antigen (ebna) are elevated in nasopharyngeal carcinoma. | the epstein-barr virus nuclear antigen i (ebna i) is the only latent ebv antigen consistently expressed in malignant tissues of the nasopharynx. a 20-amino-acid synthetic peptide, p107 contains a major epitope of ebna i. we tested sera from 210 patients with nasopharyngeal carcinoma (npc) and from 128 normal individuals (nhs) for iga antibodies to p107 using an enzyme-linked immunosorbent assay (elisa). whereas 191/210 (91%) of npc patients had iga antibodies to p107, only 17/128 (13.3%) of nhs ... | 1990 | 1693600 |
| selective virus inhibitors. | our recent efforts have been directed at the development of selective inhibitors of different classes of viruses, including adeno, pox, and herpesviruses [herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2), varicella-zoster (vzv), cytomegalovirus (cmv), epstein-barr virus (ebv)], (+/-)rna viruses (reo- and rotavirus), (-)rna viruses (influenza, parainfluenza, measles, respiratory syncytial, vesicular stomatitis and rabies virus) and retroviruses [i.e. human immunodeficiency virus (hiv), the ... | 1990 | 1693749 |
| retroviral expression in mononuclear blood cells isolated from a patient with osteopetrosis (albers-schönberg disease). | we report the presence of reverse transcriptase activity in the supernatant of long-term culture of mononuclear blood cells (monocytes and lymphocytes) isolated from a 27-year-old patient suffering from benign osteopetrosis. the enzyme was purified to homogeneity according to the technique of chandra and steel, by chromatography, first on deae-cellulose (de 52) and then on phosphocellulose (p11). after purification, the enzyme was characterized biochemically for its template specificity and ioni ... | 1990 | 1695060 |
| cloned stromal cell lines derived from human whitlock/witte-type long-term bone marrow cultures. | we report a human bone marrow culture technique that initially parallels the murine whitlock/witte culture system. as in the murine system, b cells predominate over other cell types, and all differentiation stages from pre-b to plasma cell are observed. although these human long-term cultures pass through stages resembling phases i to iii of murine whitlock/witte cultures, no outgrowth of nonadherent cells was seen after cultures had reached the "crisis" phase unless epstein-barr virus (ebv)-tra ... | 1990 | 1696206 |
| evidence for diversity of plasmodium falciparum sporozoite surface antigens derived from analysis of antibodies elicited in humans. | we have compared the reactivities of antibodies developed by individuals frequently exposed to plasmodium falciparum infections with the epitopes contained within the repeats of the circumsporozoite (cs) protein and their reactivities with the epitopes of a native molecule(s) accessible on the sporozoite surface. results of direct-binding assays and competition assays between artificial and native molecules or between human antibodies and anti-cs monoclonal antibodies suggest that humans respond ... | 1990 | 1696936 |
| monoclonal autoantibodies from insulin-dependent diabetic patients: autoantibodies against beta-cell surface or cytoplasmic antigens. | to investigate the target antigen(s) recognized during the autoimmune process in insulin-dependent diabetes mellitus (iddm), we produced human monoclonal antibodies by epstein-barr virus transformation of peripheral blood lymphocytes from a large number (n = 50) of newly diagnosed iddm patients. screening by indirect immunofluorescence assay, using the rinm5f rat insulinoma cell line and eight other human or rat tumour cell lines, was performed to identify monoclonal antibodies that reacted with ... | 1990 | 1698582 |
| soluble cd23 containing b cell supernatants induce ige from peripheral blood b-lymphocytes and costimulate with interleukin-4 in induction of ige. | the role of soluble fragments of cd23 and their relationship to interleukin-4 (il-4) in the in vitro production of ige by normal human peripheral blood mononuclear cells was examined. most donors' cells were induced to produce ige in vitro by il-4 during a 9- to 21-day culture. this stimulation was not observed in the absence of t cells. inability of il-4 to induce ige in nonresponding cultures was associated with a failure to express cd23 on lev-19+ natural killer cells; cd23 expression on b ce ... | 1990 | 1698844 |
| a high-affinity human monoclonal igm antibody reacting with multiple strains of mycoplasma hominis. | human monoclonal antibodies were produced against mycoplasma hominis by in vitro immunization of peripheral blood lymphocytes from a healthy seropositive donor using low amounts of antigen (5 ng/ml). the immune b lymphocytes were subsequently immortalized by epstein-barr virus transformation followed by somatic cell fusion. using this procedure, several specific anti-m. hominis monoclonal antibodies of mu isotype were obtained exhibiting apparent affinity constants as high as 3 x 10(9)m-1. the p ... | 1990 | 1700092 |