| cloning and expression of the major 47-kilodalton surface immunogen of treponema pallidum in escherichia coli. | monoclonal antibodies directed against the 47-kilodalton (kda) major outer membrane surface immunogen of virulent treponema pallidum subsp. pallidum were used to select escherichia coli recombinant clones expressing the 47-kda immunogen. the phenotype of the clones was dependent on the presence of recombinant plasmid in the host cell. southern hybridization revealed that the cloned t. pallidum subsp. pallidum dna sequence was an accurate representation of the t. pallidum subsp. pallidum genomic ... | 1986 | 3021631 |
| activity of sulbactam/ampicillin in screening and discriminative animal models of infection. | the efficacy of sulbactam/ampicillin in the treatment of mice with fatal systemic infections produced by ampicillin-resistant staphylococcus aureus, haemophilus influenzae, klebsiella pneumoniae, or proteus vulgaris strains is well established. in this paper the demonstrations of efficacy for sulbactam/ampicillin have been extended to a number of clinically relevant models, including bacteremia and meningitis produced by h. influenzae in infant rats, experimental staphylococcal endocarditis in r ... | 1986 | 3026002 |
| a plasmid expression vector that permits stabilization of both mrnas and proteins encoded by the cloned genes. | two new expression vectors have been constructed to take advantage of several useful properties of bacteriophage t4-infected escherichia coli. these plasmids, prdb8 and prdb9, contain the promoter region and start codon of t4 gene 32, a contiguous multiple cloning site (mcs), and translation and transcription termination signals. dna fragments inserted into the mcs are transcribed and translated at a high level in both uninfected and phage t4-infected cells. furthermore, the extreme stability of ... | 1986 | 3026907 |
| posttranslational processing of an epstein-barr virus-encoded membrane protein expressed in cells transformed by epstein-barr virus. | the bamhi nhet fragment of the b958 strain of epstein-barr virus (ebv) encodes a membrane protein (bnlf-1) that is present in cells transformed by ebv. we made a hybrid protein in which a polypeptide sequence from the carboxyl-terminal part of bnlf-1 is fused to escherichia coli beta-galactosidase. this hybrid protein was used to immunize rabbits, and the resulting antiserum was purified by immunoaffinity chromatography. the antiserum was able to immunoprecipitate bnlf-1 from cell lysates. we fo ... | 1987 | 3027413 |
| in situ distribution of ecori methylase and restriction endonuclease in cells of escherichia coli bs 5. | specific igg antibodies were raised in rabbits against purified ecori methylase and restriction endonuclease. post embedding labeling experiments, using the protein a-gold technique, were made with paraformaldehyde-glutaraldehyde fixed cells, embedded in lowicryl k4m resin at low temperatures. labeling with methylase-specific antibodies showed 60-70% of gold particles in the cytoplasm and 30-40% at the cell envelope, whereas the use of restriction enzyme-specific antibodies led to a distribution ... | 1987 | 3034675 |
| inhibition of attachment of escherichia coli rdec-1 to intestinal microvillus membranes by rabbit ileal mucus and mucin in vitro. | intestinal mucus is postulated to play a role in preventing colonization of the gastrointestinal tract by microbial pathogens. to evaluate the ability of both crude mucus and purified mucin, a glycoprotein of goblet cell origin, to inhibit mucosal adherence of enteric pathogens, we examined whether mucus and mucin derived from rabbit ileum interact with the rabbit enteropathogen escherichia coli rdec-1. we examined the manner in which mucus and mucin inhibited adherence of bacteria to rabbit ile ... | 1988 | 3045000 |
| two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues. | using a monoclonal antibody to macrophage migration inhibition factor (mif), two proteins were isolated from supernatants of concanavalin a-stimulated human peripheral blood mononuclear cells which seem to have complexed to a third component carrying the mif activity. they are therefore designated mif-related proteins or mrp-8 and mrp-14 according to their apparent molecular weights. partial amino acid sequences have been determined and their cdna have been cloned and expressed in escherichia co ... | 1988 | 3048809 |
| chloroplast rpoa, rpob, and rpoc genes specify at least three components of a chloroplast dna-dependent rna polymerase active in trna and mrna transcription. | the purpose of this study was to determine the relationship between putative chloroplast rna polymerase subunit genes and known chloroplast transcriptional activities. we have prepared fusion polypeptide genes from fragments of chloroplast dna homologous to bacterial rna polymerase subunit genes and expression vectors carrying portions of the anthranilate synthetase gene (trpe). fusion proteins for chloroplast homologs of the rna polymerase alpha (rpoa), beta (rpob), and beta' (rpoc) subunits we ... | 1988 | 3049574 |
| effects of chlorpromazine, berberine and verapamil on escherichia coli heat-labile enterotoxin-induced intestinal hypersecretion in rabbit ileal loops. | the effects of chlorpromazine (cpz), berberine and verapamil on intestinal hypersecretion in the rabbit ileal loop model by the heat-labile enterotoxin (lt) of escherichia coli were studied in relation to their ability to inhibit the stimulation of intestinal adenylate cyclase (ac) by lt. cpz 5 mg by the intraluminal (i.1.) route and 4 mg/kg by the intramuscular (i.m.) route significantly reduced lt-induced intestinal hypersecretion. berberine (10 mg) exerted an inhibitory effect, but only after ... | 1988 | 3050115 |
| toxins and serotypes of faecal non-enterotoxigenic and non-enteropathogenic escherichia coli strains causing mannose-resistant haemagglutination: relation with haemagglutination patterns. | forty-three faecal non-enterotoxigenic and non-enteropathogenic human escherichia coli strains causing mannose-resistant haemagglutination (mrha) were tested for production of cytotoxic necrotizing factor (cnf), haemolysis (hly), verotoxin (vt) and lethal activity for mice. the serotypes of the strains were also determined. of the total strains investigated, 49% synthesized cnf, 53% were haemolytic and 40% were lethal for mice. no strain producing vt was detected. striking differences in the pro ... | 1988 | 3051801 |
| global variation in g+c content along vertebrate genome dna. possible correlation with chromosome band structures. | the global, rather than local, variation in g+c content along the nuclear dna sequences of various organisms was studied using genbank sequence data. when long dna sequences of the genomes of escherichia coli and saccharomyces cerevisiae were examined, the levels of their g+c content (g+c%) were found to be within a narrow range around that of the whole genome. the g+c% levels for sequences of vertebrate genomes, however, were found to cover a wide range, showing that their genome is a mosaic of ... | 1988 | 3054117 |
| antiserum to endotoxin in hemorrhagic shock. | antiserum to escherichia coli j5, a mutant endotoxin (lps) which contains only core determinants, has proven effective in reducing mortality from endotoxic shock due to a wide variety of gram-negative bacteria. twenty new zealand white rabbits with coliforms in the gut were subjected to hemorrhagic shock of 36 mm hg for 3 hr. treated rabbits were resuscitated with 15 cc of rabbit j5 antiserum (hemagglutinating antibody titer against j5 lipopolysaccharide of 1:1024), remaining shed blood, and lac ... | 1988 | 3054323 |
| efficient in vitro expression of interferon alpha analogs using sp6 polymerase and rabbit reticulocyte lysate. | we have investigated the use of in vitro expression as a quick and convenient means of screening large numbers of interferon (ifn) analogs generated using in vitro mutagenesis. the ifn-alpha 1 mrna generated from dna template using sp6 rna polymerase is efficiently translated in rabbit reticulocyte lysate (rrl). the antiviral specific activity of this rrl-synthesized ifn-alpha l is equivalent to the yeast-synthesized protein. in contrast with the yeast-expression system, where some ifn-alpha ana ... | 1988 | 3056802 |
| reconstitution of mitochondrial protein transport with purified ornithine carbamoyltransferase precursor expressed in escherichia coli. | ornithine carbamoyltransferase (otc; subunit, 36,000 da) is initially synthesized as a precursor (potc) with a transient nh2-terminal presequence of 32 amino acid residues and imported posttranslationally into the mitochondrial matrix. the rat potc was synthesized in escherichia coli using an expression vector containing a thermoinducible lambda pl promoter. the recombinant potc represented 5-10% of the total bacterial protein and was present in the precipitate of the disrupted bacteria. the pre ... | 1988 | 3056942 |
| inhibition of protein synthesis by a vero toxin (vt2 or shiga-like toxin ii) produced by escherichia coli o157:h7 at the level of elongation factor 1-dependent aminoacyl-trna binding to ribosomes. | a vero toxin (vt2 or shiga-like toxin ii) from escherichia coli o157:h7 was shown to inhibit protein synthesis in a rabbit reticulocyte lysate, but not in wheat germ or ercherichia coli lysates. the toxin, vt2, inactivated 60s ribosomal subunits of rabbit reticulocytes. the site of inhibition of protein synthesis by vt2 was shown to be elongation factor 1-dependent aminoacyl-trna binding to ribosomes. vt2 did not affect met-trnaf binding to ribosomes, non-enzymatic binding of aminoacyl-trna to r ... | 1988 | 3059137 |
| neurotoxicity of benzylpenicillin in experimental escherichia coli meningitis. | the neurotoxic potential of benzylpenicillin administered intravenously as a continuous infusion was studied in rabbits with experimental escherichia coli meningitis. as controls a group of rabbits was injected with saline into the cisterna magna. the concentrations of benzylpenicillin in serum, csf and brain tissue fluid were studied at onset of epileptogenic electroencephalographic activity (thirteen rabbits) or convulsions (ten rabbits), with a previously developed method for neurotoxicity st ... | 1988 | 3060460 |
| endotoxin, cerebral blood flow, amino acids and brain damage in young rabbits. | the effects of bacterial endotoxin (lipopolysaccharide; lps) on the cerebral blood flow (cbf), amino acid levels and brain histology were studied in young rabbits. the cbf was slightly decreased in the cerebral cortex and markedly decreased in the cerebral white matter at 60 and 120 min after lps administration. histological examination revealed only slightly pyknotic neurons around small vessels at 24 hours, and multifocal necrosis in the deep cerebral cortex and white matter at 72 hours. some ... | 1988 | 3064628 |
| analysis of the class-specific immune response to yersinia enterocolitica virulence-associated antigens in oro-gastrically infected rabbits. | the antibody response was analysed in rabbits oro-gastrically infected (i) with virulent-(plasmid-carrying) yersinia enterocolitica 0:3 (v-rabbits) and (ii) with the avirulent (plasmid-cured) derivative (av-rabbits). in an immunoblot assay with whole cell lysate proteins from the infecting virulent yersinia strain, a significant igg response was evident in convalescent-sera of v-rabbits and of av-rabbits, demonstrating that all rabbits seroconverted. however, v-rabbits mounted a stronger immune ... | 1988 | 3072465 |
| [enterotoxin-like factor(s) produced by vibrio parahaemolyticus]. | culture filtrates obtained from several isolates of vibrio parahaemolyticus were tested for their enterotoxin-like activities. also their antigenic relationship with cholera enterotoxin and kanagawa hemolysin were tested. in the chinese hamster ovary (cho) cells assay which are usually employed for detecting cholera toxin or heat labile enterotoxin produced by enterotoxigenic escherichia coli, crude culture filtrates obtained from both kanagawa positive and negative strains isolated from patient ... | 1986 | 3084368 |
| identification of brucella abortus in formalin-fixed, paraffin-embedded tissues of cows, goats, and mice with an avidin-biotin-peroxidase complex immunoenzymatic staining technique. | an avidin-biotin-peroxidase complex immunoenzymatic staining technique was evaluated for light microscopic detection of brucella organisms in formalin-fixed, paraffin-embedded tissues. tissues from cows, goats, and mice inoculated with b abortus strain 2308 were examined, using rabbit antiserum to brucella cell surface protein as primary antibody. stained organisms were identified histologically in tissue sections containing b abortus, as detected by bacteriologic examination of duplicate nonpro ... | 1986 | 3096172 |
| production of specific antibodies against protein a fusion proteins. | the gene for staphylococcal protein a was fused to the coding sequence of bacterial beta-galactosidase, alkaline phosphatase and human insulin-like growth factor i (igf-i). the fusion proteins, expressed in bacteria, were purified by affinity chromatography on igg-sepharose and antibodies were raised in rabbits. all three fusion proteins elicited specific antibodies against both the inserted protein sequences and the protein a moiety. in the case of igf-i, the protein a moiety in the fusion prot ... | 1986 | 3096719 |
| [characteristics of the b subunit of a thermolabile escherichia coli enterotoxin produced by the a-b+ e. coli strain]. | the preparation and identification of b subunit of thermolabile enterotoxin produced by a-b+ gene-containing strain are described. the e. coli strain studied is shown to produce protein identical in its molecular properties and antigenic specificity to b subunit obtained from the whole thermolabile enterotoxin. partial antigenic affinity between b subunits of thermolabile enterotoxin obtained from different sources and b subunit from cholera enterotoxin has been established in immunochemical stu ... | 1987 | 3099858 |
| ceftazidime and amikacin alone and in combination against pseudomonas aeruginosa and enterobacteriaceae. | the efficacy of ceftazidime alone and combined with amikacin was studied in a rabbit model simulating closed-space infections at locally neutropenic sites. six strains of pseudomonas aeruginosa, and six enterobacteriaceae (two strains each of klebsiella pneumoniae and serratia marcescens and one strain each of escherichia coli and citrobacter freundii) in pooled rabbit serum were each inoculated into separate subcutaneous semipermeable chambers. intramuscular antibiotic therapy was begun 4 hr la ... | 1987 | 3100127 |
| induction of meningeal inflammation by diverse bacterial cell walls. | | 1986 | 3100293 |
| molecular cloning and expression of neisseria meningitidis class 1 outer membrane protein in escherichia coli k-12. | a genomic library of meningococcal dna from a clinical isolate of neisseria meningitidis was constructed in the expression vector lambda gt11. outer membrane complex was prepared from the same strain and used to immunize rabbits to raise polyclonal anti-outer membrane complex serum. the amplified library was probed with this polyclonal serum, and seven expressing recombinants were isolated; further investigations indicated these to be identical. the expressed meningococcal gene in these recombin ... | 1987 | 3117690 |
| expression of rabbit iga heavy chain genes in e. coli and in murine myeloma cells. | rabbit iga-heavy chain cdna and germline genes were cloned into prokaryotic and eukaryotic expression vectors, respectively. the fc alpha encoding portion of six c alpha cdna clones were cloned into puc8 and e. coli were transformed. radioimmunoassay of the molecules synthesized by these clones showed that molecules with fc alpha antigenic determinants were produced at the level of approximately 0.1 to 1.0 microgram per ml culture. radiobinding analysis showed that each of the clones encoded hea ... | 1987 | 3124339 |
| effect of antimicrobials on blood cultures in endocarditis. | to study the effect of antimicrobials on bacterial growth in blood cultures, we used both simulated blood cultures and cultures obtained from rabbits with experimental endocarditis. four strains of bacteria were incubated individually with six antimicrobials in nine blood culture media. positivity rates varied with the ratio of the antimicrobial concentration to the mic of the organism: 161 of 162 cultures (99%) were positive when the ratio was less than 1/10; 52 of 108 (48%) were positive when ... | 1987 | 3126019 |
| demonstration of calcium-dependent phospholipase a2 activity in membrane preparation of rabbit neutrophils. absence of activation by fmet-leu-phe, phorbol 12-myristate 13-acetate and a-kinase. | the presence of a phospholipase a2 (pla2) activity in rabbit neutrophil membrane preparation that is able to release [1-14c]oleic acid from labelled escherichia coli has been demonstrated. the activity is critically dependent on the free calcium concentration and marginally stimulated by gtp gamma s. more than 80% of maximal activity is reached at 10 microm-ca2+. the chemotactic factor, fmet-leu-phe, does not stimulate the pla2 activity in this membrane preparation. pretreatment of the membrane ... | 1988 | 3128281 |
| endogenous tumor necrosis factor induction with bordetella pertussis vaccine as a triggering agent and its therapeutic effect on mm46 carcinoma-bearing mice. | induction of endogenous tumor necrosis factor (tnf) by administration of bordetella pertussis vaccine (bpv) as a triggering agent and its therapeutic effect against mm46 carcinoma were investigated in c3h/he mice. test triggering agents were injected intravenously into mice after intravenous injection of 4-fold dilution of macrophage activating factor (maf) or 10(4) units of murine interferon-gamma (mu-ifn-gamma). then sera were obtained from the mice, and their tnf activities were assayed on l- ... | 1988 | 3131286 |
| synthesis and secretion of multiple forms of beta 2-interferon/b-cell differentiation factor 2/hepatocyte-stimulating factor by human fibroblasts and monocytes. | the cdna for human beta 2-interferon (ifn-beta 2)/b-cell differentiation factor 2/hepatocyte-stimulating factor was expressed in escherichia coli to yield a fusion protein which contains the 182 carboxyl-terminal amino acids of ifn-beta 2 fused to a 34-amino acid prokaryotic leader peptide (rifn-beta 2). when added to cultures of human hepatoma cell line hep3b2, rifn-beta 2 as well as preparations of natural ifn-beta 2 enhance secretion of positive acute phase reactants such as alpha 1-antichymo ... | 1988 | 3131326 |
| chemical and immunological characterization of the 21-kda adp-ribosylation factor of adenylate cyclase. | the adp-ribosylation factor (arf) is a 21-kda gtp-binding protein cofactor in the cholera toxin-catalyzed adp-ribosylation of the stimulatory regulatory subunit of adenylate cyclase. purified bovine brain arf was digested with cyanogen bromide, and peptides were purified and sequenced. approximately 25-30% of the protein was sequenced in this manner. peptides contained consensus sequences for gtp-binding proteins but were distinct from any of the previously published gtp-binding proteins. antibo ... | 1988 | 3131341 |
| immunogenic and antigenic characteristics of salmonella heat-labile enterotoxin. | the intramuscular immunization of rabbits with enterotoxin of s. weltevreden failed to provide protection against challenges with homologous salmonella enterotoxin as well as heterologous enterotoxins (cholera toxin or e. coli lt). similar results were obtained in rabbits immunized with cholera toxin, choleragenoid and e. coli lt. however, salmonella antitoxin contained neutralizing antibodies against salmonella enterotoxin (but not against cholera toxin) and thus was capable of neutralizing sal ... | 1988 | 3136609 |
| effect of antiserum against aflatoxin b1-bovine serum albumin complex on aflatoxin b1-induced lysogenesis. | escherichia coli k12 bacteria lysogenic for the lambda phage were used to study the effect of antiserum against aflatoxin b1-induced lysogenesis. the antiserum was obtained from rabbits immunized with water in oil emulsion of aflatoxin b1-bovine serum albumin complex (afb1-bsa). a marked reduction in the degree of lysogenesis was observed when the antiserum was added to the reaction medium prior to microsomal enzyme activation of aflatoxin b1. there was no detectable effect when the antiserum wa ... | 1988 | 3140005 |
| effects of stroma-free hemoglobin solutions on isolated perfused rabbit hearts and isolated perfused rat kidneys. | "stroma-free" hemoglobin solutions (sfh) cause hemodynamic alterations indicative of vasoconstriction. we studied vasoconstrictor activity in isolated rabbit hearts and rat kidneys of unmodified sfh and of sfh modified by pyridoxylation or glyoxylation, with or without glutaraldehyde cross-linking. the purity and chemical composition of the solutions, all prepared by other laboratories, were not characterized by us. in isolated hearts sfh prepared by conventional methods had potent vasoconstrict ... | 1988 | 3140918 |
| purification and some properties of shiga-like toxin from escherichia coli o157:h7 that is immunologically identical to shiga toxin. | a cytotoxin to vero cells (shiga-like toxin), which was neutralized by antibody against purified shiga toxin produced by shigella dysenteriae 1, was purified from escherichia coli o157:h7, isolated from a patient with hemorrhagic colitis. the purification procedure consisted of ammonium sulfate fractionation, deae-cellulose column chromatography, chromatofocusing column chromatography and high performance liquid chromatography. about 200 micrograms of purified shiga-like toxin was obtained from ... | 1987 | 3148810 |
| evolution of catalytic and regulatory sites in phosphorylases. | glycogen phosphorylase (e.c.2.4.1.1) was the first enzyme shown to be regulated by allosteric effectors and by protein phosphorylation. transcriptional control of bacterial phosphorylases further extends the range of regulatory mechanisms by which phosphorylases contribute to the control of carbohydrate metabolism. despite their regulatory differences, all known phosphorylases share catalytic and structural properties and a strongly conserved pyridoxal-5'-phosphate binding site; this makes phosp ... | 1985 | 3155826 |
| clinical significance and pathogenesis of hyperbilirubinemia associated with staphylococcus aureus septicemia. | our goal was to examine the clinical significance of hyperbilirubinemia in patients with staphylococcus aureus endocarditis. in addition, preliminary data concerning the possible mechanism of cholestasis observed during s. aureus septicemia are presented. | 1988 | 3189364 |
| simple serological tests for detecting classical heat labile enterotoxin (lt-i) of escherichia coli. | diarrhoea caused by enterotoxigenic escherichia coli (etec) remains a problem in southeast asia. at present, no routine laboratories as yet are available for etec detection. in this study, attempts were made to produce reagents for use in simple serological tests for detecting lt. the serological methods were the biken, the staphylococcal coagglutination and the reverse passive hemagglutination tests. for the biken test, medium was prepared locally by mixing constituents as described previously ... | 1988 | 3227397 |
| ocular inflammatory effects of intravitreally-injected tumor necrosis factor. | many of the pathophysiologic effects of bacterial endotoxin have recently been attributed to a monokine, tumor necrosis factor (tnf). the rabbit eye is extremely sensitive to locally injected endotoxin. the authors have investigated the possible contribution of tnf to ocular inflammation in a rabbit model. the intravitreal injection of 10(5) to 5 x 10(5) units of recombinant human tnf produced a sustained disruption of the blood-aqueous barrier as manifested by elevated aqueous humor protein lev ... | 1988 | 3263050 |
| [ultrastructural changes in the microcirculatory bed of the lungs in endotoxin shock]. | the electron microscopic study of the lungs during the initial and intermediate stages of endotoxin shock has revealed the manifestations of thrombo-hemorrhagic syndrome. three days later interstitial fibrosis developed. ultrastructural alterations of microcirculation become the basis for the formation of acute pulmonary failure. | 1988 | 3280052 |
| rabbit pasteurellosis: induced disease and vaccination. | pasteurellosis was induced in rabbits by conjunctival inoculation with 2 strains of pasteurella multocida. the ld50 of strain p1062 (a bovine isolate) was 10(5.1) colony-forming units and that of strain p1059 (a turkey isolate) was 10(5.5) colony-forming units. pasteurella-free rabbits were vaccinated iv or mucosally with boiled cells of p multocida or a cross-reactive uridine diphosphogalactose epimerase-deficient mutant of escherichia coli j5. in rabbits challenge exposed with p multocida stra ... | 1988 | 3282457 |
| endotoxin neutralization with rabbit antisera to escherichia coli j5 and other gram-negative bacteria. | to study the mechanisms of protection against endotoxin challenge offered by antisera to smooth and rough gram-negative organisms, we have developed an assay to quantitate endotoxin neutralization based on inhibition of the limulus amoebocyte lysate test. dilutions of different bacterial lipopolysaccharides (lpss) were incubated with hyperimmune rabbit sera against escherichia coli o113, e. coli o18, and rough mutants e. coli j5 and salmonella minnesota re595 and were then combined with limulus ... | 1987 | 3298063 |
| the effects of escherichia coli endotoxin as a trigger for hepatic infection of rabbits with fusobacterium necrophorum. | to evaluate the effects of endotoxin in hepatic infection with fusobacterium necrophorum, rabbits were given several combinations of f. necrophorum and escherichia coli endotoxin. severe hepatic necrosis with multiplication of f. necrophorum was induced by inoculation of endotoxin via the bile duct and following inoculation of both endotoxin and f. necrophorum. inoculation with f. necrophorum alone, preceded by inoculation of endotoxin via the bile duct, also induced hepatic necrosis, whereas ra ... | 1987 | 3298332 |
| an immunological assay for chloramphenicol acetyltransferase. | we report the production and utility of rabbit polyclonal antisera to the bacterial protein chloramphenicol acetyltransferase (cat). the anti-cat antibodies specifically react with cat protein produced in bacterial or avian cells. results reported here demonstrate that detection of cat by immuno-dot blot techniques is at least as sensitive as the currently used in vitro enzymatic assay. the availability of antibodies to cat offers a number of potential advantages to investigators who now use the ... | 1987 | 3300416 |
| enteric infections caused by non-enterotoxigenic escherichia coli in animals: occurrence and pathogenicity mechanisms. a review. | not all e. coli that cause diarrhoea in farm animals act by elaborating the classical heat-labile or heat-stable enterotoxins. these newly recognised animal enteropathogenic e. coli (epec) attach to and efface the microvilli of the gut epithelium and resemble the well known human epec. in rabbits, only this type of e. coli enteritis is known to be important and a similar disease has been shown to occur in cattle also. there is no doubt that adherence factors are important in the pathogenesis of ... | 1987 | 3307126 |
| [lyophilization of live ca-80 vaccine against coli enteritis in pigs]. | a live vaccine against coli enteritis in pigs, ca-80, with a protection medium of 5 per cent hemodex, was successfully freeze-dried in ampoules or vials at the rate of 5 to 10 vaccinal doses each, the titer ranging from 10(9) to 10(10) live bacteria per cu. cm, of a 12-month shelf-life period. the vaccine was found to be innocuous for pigs, and was intended for active immunoprophylaxis on both infected and menaced farms and complexes, applying it to sows in the last third of the gestation period ... | 1987 | 3307134 |
| antibody to endotoxin core glycolipid reverses reticuloendothelial system depression in an animal model of severe sepsis and surgical injury. | to study the effect of severe sepsis on the function of the reticuloendothelial system (res) we have measured the clearance kinetics and organ distribution of both low-dose technetium tin colloid (ttc) and 75selenomethionine-labelled e. coli in rabbits 24 hours after either sham laparotomy or appendix devascularization. sepsis resulted in similar delayed blood clearance and reduced liver (kupffer cell) uptake of both ttc and e. coli. to investigate the ability of polyclonal antibody to e. coli-j ... | 1987 | 3312624 |
| heterologous expression and characterization of the human r-ras gene product. | we directly expressed human r-ras 23,000-dalton protein (p23) cdna in escherichia coli under the control of the trp promoter. gtp-dependent phosphorylation of a p23 threonine 85 substitution mutant was observed. this result is in direct analogy to the autokinase activity of h-ras and k-ras threonine 59 substitution mutants. normal p23 protein was detected in the human fibrosarcoma cell line ht1080 by immunoprecipitation with rabbit antibodies raised against an e. coli-expressed r-ras fusion prot ... | 1987 | 3313005 |
| cloning structural genes for treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, p2 (p2 star). | a genomic library consisting of partially digested 10 to 20 kilobase pair fragments of treponema pallidum deoxyribonucleic acid (dna) was constructed using bacteriophage lambda embl-3 as the vector. positive clones expressing t pallidum antigens were detected with sera from experimentally infected rabbits. treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage ... | 1987 | 3315959 |
| thermoregulation of the rabbit during the late phase of endotoxin fever. | in the late phase of the fever occurring 120 or more min after i.v. injection of endotoxin (1 microgram/kg) to female rabbits, marked shifts of thresholds for respiratory evaporative heat loss and for peripheral vasodilatation to higher body core temperatures were observed. in contrast, the threshold body core temperature for cold thermogenesis was shifted downwards. as a result, the interthreshold zone was widened. within the body temperature range of 37.4 to 39.9 degrees c neither heat product ... | 1987 | 3317270 |
| production of recombinant human pancreatic secretory trypsin inhibitor by escherichia coli. | a synthetic gene for human pancreatic secretory trypsin inhibitor (psti) was fused to the coding sequence for the amino-terminal 135 amino acid residues of human interferon-gamma (ifn-gamma) by interposing a methionine codon sequence, and the resulting hybrid gene was efficiently expressed in escherichia coli cells. recombinant human psti (rhu-psti) was separated from the ifn-gamma/psti fused protein by cleavage at the methionine residue with cyanogen bromide. finally, rhu-psti was purified by a ... | 1987 | 3323193 |
| antipyretic activity of copper salicylate. | thermal responses to copper ii salicylate (200 mg/kg iv) were investigated in febrile rabbits (treated with e. coli lipopolysaccharide) at 3 ambient temperatures (ta) of 5, 20, 28 degrees c. the compound produced antipyresis, which unlike that induced by sodium salicylate (200 mg/kg iv), increased with the drop of ta. this antipyretic activity was accompanied by enhanced heat elimination which became most evident in thermoneutral conditions. the metabolic rate was intensified after treatment wit ... | 1987 | 3325952 |
| chemical probing of adenine residues within the secondary structure of rabbit 18s ribosomal rna. | the location of unpaired adenine residues within the secondary structure of rabbit 18s ribosomal rna was determined by chemical probing. naked 18s rrna was first prepared by digestion of purified 40s subunits with matrix-bound proteinase k in sodium dodecyl sulfate, thereby omitting the use of nucleic acid denaturants. adenines within naked 18s rrna were chemically probed by using either diethyl pyrocarbonate or dimethyl sulfate, which specifically react with unpaired nucleotides [peattie, d. a. ... | 1988 | 3349049 |
| effect of antibody-mediated neutropenia on the cardiopulmonary response to endotoxemia. | an ovine anti-neutrophil antibody has been produced by immunizing rabbits with purified sheep neutrophils. serial intraarterial infusions of anti-neutrophil antibody in awake instrumented sheep produced selective and profound neutropenia. intravascular infusion of endotoxin (escherichia coli, 1.5 micrograms/kg/30 min) resulted in significant and equivalent increases in pulmonary artery pressure, peripheral vascular resistance, and protein-rich pulmonary lymph flow in an endotoxin group (n = 9) a ... | 1988 | 3411950 |
| antipyretic activity of a human immunoglobulin preparation for intravenous use in an experimental model of fever in rabbits. | in an effort to elucidate the reason that fever in patients with severe bacterial infections subsided in some cases after the administration of human immunoglobulin preparations for intravenous use (igivs), we focused our attention on the antipyretic activity of igivs by investigating experimentally produced pyrexia in rabbits with escherichia coli-derived lipopolysaccharide (lps). although little difference in antibody titers against the antigens composing molecules of lps was found among the i ... | 1987 | 3493219 |
| serospecificity of a cloned protease-resistant treponema pallidum--specific antigen expressed in escherichia coli. | we evaluated the serological reactivity of a protease-resistant antigen designated 4d which was encoded by treponema pallidum dna and was expressed in escherichia coli from recombinant plasmid paw329. this 19,000-molecular-weight antigen was purified in its native, non-protease-treated form from e. coli sonic extracts by molecular sieving and ion-exchange chromatography. antibody binding to antigen 4d was detected by a radioimmunoassay. antigen 4d-specific antibody was detected in 95% of the ser ... | 1986 | 3514656 |
| native surface association of a recombinant 38-kilodalton treponema pallidum antigen isolated from the escherichia coli outer membrane. | a recombinant plasmid designated paw305, containing a 6-kilobase insert of treponema pallidum dna, directed the expression of a 38-kilodalton (kda) treponemal antigen in escherichia coli. the 38-kda antigen copurified with the outer membrane fraction of the e. coli cell envelope after treatment with nonionic detergents or sucrose density gradient centrifugation. rabbits immunized with the recombinant 38-kda antigen developed antibodies which reacted specifically with a 38-kda t. pallidum antigen ... | 1986 | 3516880 |
| kinetics and bactericidal effect of gentamicin and latamoxef (moxalactam) in experimental escherichia coli endocarditis. | the pharmacokinetics of gentamicin and latamoxef (moxalactam) were examined in serum, normal heart valves, sterile cardiac vegetations and vegetations infected with escherichia coli. penetration of antibiotics into heart valves and vegetations was rapid; the maximum concentration was achieved in both sites at the end of a 20 min iv infusion. however, both antibiotics penetrated better into vegetations than into normal heart valves. in rabbits with left-sided endocarditis, similar antibiotic leve ... | 1986 | 3516963 |
| the role of lipoproteins and receptor-mediated endocytosis in the transport of bacterial lipopolysaccharide. | the addition of bacterial lipopolysaccharide (lps) from escherichia coli 0111:b4 to human monocyte-macrophages cultured in serum results in suppression of scavenger receptor activity. the present studies were performed to examine if the effect on scavenger receptor activity was mediated by lps alone or by lps in association with lipoproteins. radioiodinated lps (125i-lps) was added to human plasma in vitro and to normal and hyperlipidemic rabbit plasma in vitro and in vivo to determine the distr ... | 1986 | 3517876 |
| [elimination of klebsiella pneumoniae and humoral immunity in immunization with the is-4 immunostimulating mixture]. | the present investigation has revealed that in mice, immunized with preparation hc-4 (an immunostimulating agent consisting of water-soluble antigenic complexes obtained from 4 opportunistic microorganisms: klebsiella pneumoniae, staphylococcus, proteus and escherichia coli k-100 having a common antigen with haemophilus influenzae) and challenged with k. pneumoniae culture on day 7 after immunization, the complete elimination of k. pneumoniae from the blood occurs within 24 hours. the subcutaneo ... | 1986 | 3518307 |
| removal of endotoxin from water by microfiltration through a microporous polyethylene hollow-fiber membrane. | the microporous polyethylene hollow-fiber membrane has a unique microfibrile structure throughout its depth and has been found to possess the functions of filtration and adsorption of endotoxin in water. the membrane has a maximum pore diameter of approximately 0.04 micron, a diameter which is within the range of microfiltration. approximately 10 and 20% of the endotoxin in tap water and subterranean water, respectively, was smaller than 0.025 micron. endotoxin in these water sources was efficie ... | 1986 | 3518630 |
| molecular cloning of the gene encoding rabbit tumor necrosis factor. | a rabbit genomic dna library was screened using a cloned cdna encoding rabbit tumor necrosis factor (tnf) as a probe. the entire gene sequence appears to be contained within a 3.2-kb eco ri fragment. comparison of the nucleotide sequence of the gene with that of the cdna showed that the gene consists of four exons. the nucleotide sequence of the rabbit tnf gene is very homologous to that of the human tnf gene. southern hybridization of the genomic dnas showed that there is likely only a single t ... | 1986 | 3519138 |
| [detection of endotoxins in radiopharmaceutical preparations--i. comparison of rabbit hyperthermia after intravenous or intrathecal administration of reference endotoxin preparations]. | the rise of the rabbit internal temperature after endotoxin injection is related to the route of administration. a rise of 1.71 +/- 0.411 degrees c is obtained after i.v. injection of 1 ng/kg escherichia coli 0111.b.4 endotoxin. an increase of 1.93 +/- 0.236 degrees c is obtained after suboccipital intrathecal injection of 0.1 ng/kg of the same endotoxin; with the intrathecal route, the hyperthermia is induced by e. coli endotoxin after a dose ten times lower than with i.v. injection as shown by ... | 1986 | 3519488 |
| [experimental ulcerative colitis induced by escherichia coli in rabbits]. | | 1986 | 3522965 |
| hemagglutination induced by lipopolysaccharides and lipid a. | | 1986 | 3523165 |
| neutropenic responses to intradermal injections of escherichia coli. effects on the kinetics of polymorphonuclear leukocyte emigration. | killed escherichia coli organisms injected intradermally into rabbits induced significant neutropenia and provoked a rapid rise in body temperature. both the magnitude and the duration of the neutropenia were dose-dependent. after recovery from neutropenia, the rabbits became refractory to its redevelopment when subsequently given an equivalent dose of e coli. the influence of neutropenia and the subsequent refractory period on the rate of polymorphonuclear leukocyte (pmn) emigration into inflam ... | 1986 | 3524251 |
| expression of reovirus p14 in bacteria and identification in the cytoplasm of infected mouse l cells. | reovirus genome segment s1 is transcribed by the virion-associated polymerase to form a single mrna species that codes for two polypeptides: the 49-kda cell-attachment protein, sigma 1, starting from the first a-u-g in the s1 transcript, and a 14-kda nonstructural, basic protein initiated from the second a-u-g in a different reading frame (ernst and shatkin, 1985; jacobs et al., 1985; shatkin, 1985). to confirm that p14 is made in reovirus-infected cells, determine its intracellular location, an ... | 1986 | 3526708 |
| significance of non-pathogenic cross reactive bowel flora in patients with ankylosing spondylitis. | we have previously shown that antisera raised in rabbits to certain enteric cross reactive strains of bacteria are capable of specifically lysing the peripheral blood lymphocytes of hla-b27 positive patients with ankylosing spondylitis (b27+ as+). we now report that bacteria with cross reactive antigenic determinants are found in the bowel flora of all of 52 b27+ as+ patients but in only one of 50 hla-b27 positive normal controls (b27+ as-). these organisms are functionally similar to the cross ... | 1986 | 3527085 |
| immunoadsorbent-purified antibodies in the study of antigenic relatedness of outer membrane proteins of enteric bacilli. | immunoadsorbent chromatography was used for purification of antibodies to e. coli 055 outer membrane proteins. antibodies to the 33.5 kd and 7.5 kd proteins were eluted when rabbit antisera were applied to an epoxy-activated sepharose 6b column to which the outer membrane was coupled in the presence of dioxane. elisa coats prepared with sonicated bacteria showed binding of the eluted antibodies with strains of all of seven different species of the enteric bacilli, but not with other gram-negativ ... | 1986 | 3529814 |
| enhancement of slow-wave sleep by endotoxin and lipid a. | some muramyl peptides derived from bacterial peptidoglycan enhance slow-wave sleep (sws). the purpose of this study was to test whether another cell wall component, lipopolysaccharide (lps), and its lipid a moiety also have an effect on sleep. when injected intravenously, both lps and lipid a enhanced the duration of sws, increased electroencephalogram delta-wave amplitudes, suppressed rapid eye movement (rem) sleep, and induced biphasic fevers. the effects of intravenously administered lipid a ... | 1986 | 3529990 |
| penetration of cefuzoname into the cerebrospinal fluid of rabbits. | concentrations of cefuzoname in cerebrospinal fluid (csf) were determined in a total of 16 rabbits, 5 with healthy meninges, 5 with staphylococcus aureus meningitis, and 6 with escherichia coli meningitis. mean percentages of the maximum concentration of the drug in csf versus that in serum were 0.57, 3.37, and 4.40% for healthy rabbits, those with staphylococcal meningitis, and those with e. coli meningitis, respectively. the percentages of the area under the concentration-time curve of cefuzon ... | 1986 | 3530123 |
| the role of interleukin-1 in neutrophil leukocyte emigration induced by endotoxin. | chemotactic factors induce neutrophil emigration into tissues. interleukin-1 (il-1) was found to be several log times more potent in this respect than c5a des arg, leukotriene b4, and f-met-leu-phe and of comparable potency to endotoxin. kinetic studies revealed a rapid and transient neutrophil influx, with the peak rate at 30-90 minutes. cross tachyphylaxis was observed between il-1 and endotoxin; and this, together with its high potency and rapid onset of action, suggest that il-1 mediates end ... | 1986 | 3532812 |
| emigration and accumulation of pmn-leukocytes induced by endotoxin, interleukin 1 and other chemotactic substances. | this publication describes polymorphonuclear leukocyte (pmn) emigration and accumulation, which is prerequisite for their defensive function in infected tissues. the extravasated pmns can kill microorganisms, but in this process they also release proteolytic enzymes and other cell constituents which can alter and even injure the tissues, primarily the microcirculation. in the first part of the paper in vivo quantitation of the acute inflammatory reaction is described with emphasis on pmn emigrat ... | 1986 | 3533654 |
| morphologic evaluation of the effects of shiga toxin and e coli shiga-like toxin on the rabbit intestine. | the effects of a shiga toxin derived from shigella dysenteriae type 1, strain 60r, and a shiga-like toxin from the enterohemorrhagic escherichia coli o157:h7, strain 933, were studied in the in vivo rabbit ileal loop model. the effects of both toxins were similar and resulted in severe villus blunting by 18-24 hours after exposure. with both toxins, a dose effect was noted; and the lesions, first detected at 2 hours after inoculation, became more severe over time. both toxins appeared to act dir ... | 1986 | 3535529 |
| assessment of in vivo revival, growth, and pathogenicity of escherichia coli strains after copper- and chlorine-induced injury. | cells of one enteroinvasive and three enterotoxigenic strains of escherichia coli were exposed to sublethal concentrations of copper and chlorine to produce 85 to 94% injury. injured cells were intraluminally inoculated into ligated ileal loops of anesthetized mice, and injury was assessed at timed intervals. substantial recovery (72-84%) of copper- and chlorine-injured cells was observed in the inoculated loops at 4 and 3 h, respectively. no appreciable increase in total numbers was observed du ... | 1986 | 3535675 |
| two-site column enzyme immunoassay for neuron-specific enolase (nse) in human serum using monoclonal antibodies. | a new method for the rapid determination of neuron-specific gamma-enolase (nse), gamma-subunit of alpha gamma- and gamma gamma-enolase in human serum was developed by employing monoclonal antibodies for the separation method. the assay system consists of 0.1 ml sepharose 4b column with immobilized rabbit anti-mouse igg antibodies for the separation of bound label, fab' fragments of rabbit anti-bovine gamma gamma-enolase igg labeled with beta-d-galactosidase from escherichia coli, and f(ab')2 fra ... | 1986 | 3537135 |
| [ultrastructural characteristics of the biological action of an endotoxin on sensorimotor cortical neurons]. | the work is devoted to the ultrastructural investigation of the state of the sensomotor cortex neurons after intravenous and intracisternal administration of endotoxin. the evidences are presented concerning ultrastructural alterations in mitochondria and the presence of compensatory reaction in them after intravenous injection of endotoxin. besides, coated vesicles and subsurface cisternae whose formation is induced by the endotoxin action have been studied. | 1986 | 3538577 |
| immunochemical analysis of taenia taeniaeformis antigens expressed in escherichia coli. | previously we reported the isolation of several escherichia coli clones expressing fragments of taenia taeniaeformis antigens as beta-galactosidase fused proteins (bowtell, saint, rickard & mitchell, 1984). here we describe the isolation of additional antigen-expressing clones from a larval cdna library and the assignment of these clones to 7 antigen families. these were isolated with a polyspecific rabbit antiserum raised to the oncosphere. since this serum was capable of reacting with a large ... | 1986 | 3540818 |
| the comparison of myocardial dysfunction in three forms of experimental septic shock. | a rabbit model of septic shock was used to determine if myocardial dysfunction is a common component of shock due to diverse neonatal pathogens, and prostaglandins modulate septic myocardial dysfunction. the infusion of heat-killed escherichia coli (group i), haemophilus influenzae (group ii), or staphylococcus epidermidis (group iii) produced significant decreases in the first derivative of left ventricular pressure with respect to time (p less than 0.05). each organism also produced significan ... | 1986 | 3540827 |
| uterine secretions induce phagocytosis of escherichia coli by neutrophils. | phagocytosis of escherichia coli by neutrophils was induced in the presence of uterine secretions. the phagocytosis-inducing activity of uterine secretions was not changed by heat-treatment (56 degrees c for 30 min). the phagocytosis-inducing activity of uterine secretions from rabbits treated with estradiol-17 beta was slightly higher than that from control rabbits treated with vehicle. on the other hand, administration of progesterone significantly depressed phagocytosis-inducing activity of u ... | 1986 | 3541457 |
| enzyme immunoassay for the quantitation of immunoglobulin m class antibodies to salmonella minnesota r595 and escherichia coli j5 lipopolysaccharides. | the level of human immunoglobulin (igm) in plasma specific for the lipopolysaccharide of salmonella minnesota r595 (r595 lps) and escherichia coli j5 (j5 lps) was quantitated by an enzyme immunoassay (eia) in which purified antigen is adsorbed directly onto polystyrene-acrylic copolymer cuvettes. highly purified anti-j5 and r595 lps specific igm prepared by ion-exchange resin, gel filtration, and affinity resin chromatography were used as standards. the levels of specific igm were determined in ... | 1987 | 3542358 |
| expression of staphylococcal enterotoxin c1 in escherichia coli. | the structural gene encoding staphylococcal enterotoxin c1 was cloned into escherichia coli and localized on a 1.5-kilobase hindiii-clai dna fragment by subcloning. the toxin was partially purified from e. coli clones and shown to be immunologically identical to enterotoxin c1 from staphylococcus aureus. the cloned toxin also had the same molecular weight (26,000) and charge heterogeneity as staphylococcus-derived enterotoxin. toxins from both sources were equally biologically active. | 1987 | 3542834 |
| use of mucin and hemoglobin in experimental murine gram-negative bacteremia enhances the immunoprotective action of antibodies reactive with the lipopolysaccharide core region. | an antiserum with a high content of antibodies, binding to the gram-negative lipopolysaccharide core region, was prepared by immunizing rabbits with the rough escherichia coli mutant j5. this antiserum was capable of protecting mice against lethal challenge doses of e. coli 0 111:b4 in a mouse model where the animals were compromised by means of mucin plus hemoglobin (ld 50 = 10(3) bacteria). however, no protection was observed in a non-compromised mouse model (ld 50 = 10(7) bacteria). this obse ... | 1986 | 3545072 |
| [experimental study of diarrhea induced by a strain of escherichia coli (serogroup o-103) in growing rabbits]. | the experimental infection by mouth of weaned, 6 week old rabbits was performed with escherichia coli strain o-103/10 without any adjuvant. the quantities of e. coli for the 3 experimental treatments were - none (control) - 10(4) or 10(7) per animal. the 142 rabbits were divided into 2 groups, each including the 3 treatments: a - observation of weight and eventual diarrhea during 25 days after infection; b - killing during the same period of some healthy and diarrheic animals every 2 or 3 days, ... | 1986 | 3548562 |
| structural and functional properties of a phospholipase a2 purified from an inflammatory exudate. | the cell-free supernatant of sterile inflammatory peritoneal exudates contains a phospholipase a2 that participates in the digestion of escherichia coli killed by polymorphonuclear leukocytes or by the purified bactericidal/permeability increasing protein (bpi) of these cells. this phospholipase a2 has been purified, and the sequence of the nh2-terminal 39 amino acids has been determined and compared with sequences of both bpi-responsive and bpi-nonresponsive phospholipases a2 from snake venoms ... | 1986 | 3548813 |
| passive immunization with antibodies against iron-regulated outer membrane proteins protects turkeys from escherichia coli septicemia. | escherichia coli septicemia is a common disease of young poultry and several species of mammals. rabbit antiserum was prepared against iron-regulated outer membrane proteins of e. coli. eighteen-day-old turkeys were passively immunized with antiserum and challenged by air sac inoculation of 1 x 10(6) to 2 x 10(6) cfu of e. coli o78:k80:h9. turkeys injected with normal rabbit serum or saline solution before challenge served as controls. fatalities (8 of 51 turkeys inoculated) occurred only in gro ... | 1987 | 3552995 |
| the amphiphilicity of acp helices: a means of macromolecular interaction? | acp interacts with diverse proteins in an unknown way. possibly there is a similar mode of interaction between acp and all acp-binding proteins, the amphiphilic helix. the hydrophobicities of helices from 4 different acps were compared. hydrophobic moment plots were prepared for acp helices and those of many ef hand calcium-binding proteins. both groups of proteins occupied the same region of the plot. | 1987 | 3556216 |
| association of enterotoxigenic bacteroides fragilis with diarrheal disease in young pigs. | enterotoxigenic bacteroides fragilis (etbf) was isolated from intestinal contents of 10 of 45 pigs with diarrhea. of 10 etbf-positive pigs, seven were 1 to 4 weeks of age, whereas three pigs had diarrhea after weaning (6 to 8 weeks of age). of ten pigs infected with etbf, nine were also infected with k88-positive escherichia coli, rotavirus, coronavirus, coccidia, or cryptosporidium. of 11 conventional pigs exposed to etbf at 1 to 2 days of age, two subsequently developed fatal postweaning diarr ... | 1987 | 3592377 |
| characterization of argininosuccinate lyase (ec 4.3.2.1) from chlamydomonas reinhardtii. | we have isolated and characterized argininosuccinate lyase (asl; ec 4.3.2.1) from the photosynthetic green alga, chlamydomonas reinhardtii. the general properties of chlamydomonas asl are very similar to those described previously for asls from phylogenetically diverse organisms. the algal asl has a native mr, determined by gel-filtration chromatography, of 218,000 +/- 25,000, and a pi of 5.4-5.6. the km for argininosuccinate at 37 degrees c and ph 7.5 is 0.26 mm. the subunit mr of chlamydomonas ... | 1987 | 3593239 |
| human and bovine milk: comparison of ganglioside composition and enterotoxin-inhibitory activity. | milk gangliosides inhibit vibrio cholerae enterotoxin and escherichia coli heat-labile enterotoxin. human milk gangliosides showed considerably higher enterotoxin-inhibitory activity compared to bovine and formula milk gangliosides as measured in vitro by enzyme-linked immunosorbent assay and in vivo in rabbit small bowel loops. while gangliosides from less than 1 ml human milk inhibited 0.1 microgram choleratoxin in vitro and in vivo, five to 10 times higher amounts of bovine milk gangliosides ... | 1986 | 3714350 |
| the complete amino acid sequence of potato alpha-glucan phosphorylase. | the complete amino acid sequence of potato alpha-glucan phosphorylase has been determined. the monomer contains 916 amino acids with a molecular weight of 103,916. about one-fourth of the amino-terminal threonine is blocked by an acetyl group. sequence comparison among phosphorylases from potato tuber, rabbit muscle, and escherichia coli reveals the presence of a characteristic 78-residue insertion in the middle of the polypeptide chain of the potato enzyme. except for the large inserted portion ... | 1986 | 3722153 |
| production and characterization of monoclonal antibodies against recombinant human tumor necrosis factor/cachectin. | tumor necrosis factor is a monokine, which causes cytolysis of many transformed cells. in this study we have found that in addition to cytotoxicity recombinant escherichia coli-derived human tumor necrosis factor, like cachectin, inhibited the lipoprotein lipase of 3t3-l1 preadipocytes. both effects were inhibited by monoclonal anti-tumor necrosis factor antibodies. monoclonal antibodies against recombinant human tumor necrosis factor were produced by fusing splenocytes of immune mice with p3x63 ... | 1986 | 3729942 |
| copper salicylate complex: thermoregulatory and biochemical effects. | the antipyretic properties of copper (ii) salicylate and its effect on plasma copper, iron, zinc and ceruloplasmin concentrations was investigated in adult rabbits at an ambient temperature of 21.5 +/- 0.5 degrees c. the experiments indicated that copper salicylate (200 mg/kg/h i.v.) was a more potent antipyretic than sodium salicylate given in the same manner and doses. this pharmacological activity was found on a model of experimental fever induced by i.v. injection of lipopolysaccharide esche ... | 1985 | 3836871 |
| evaluation of bw942c, a novel antidiarrheal agent, against enterotoxins of escherichia coli and vibrio cholerae. | bw942c, an enkephalin-like pentapeptide with anti-diarrheal activity, was tested against crude toxins of escherichia coli and vibrio cholerae in the y-1 adrenal cell assay, rabbit ileal loop assay, and suckling mouse assay. the effects of bw942c on in vitro ion transport were measured in rabbit ileum mounted in ussing chambers. in vitro, bw942c decreased basal short-circuit current (2.26 and 3.15 mueq cm-2 h-1 in experimental samples and controls, respectively; n = 7, p less than 0.05) and incre ... | 1985 | 3838969 |
| molecular cloning of the complementary dna for human tumor necrosis factor. | tumor necrosis factor (tnf) is a soluble protein that causes damage to tumor cells but has no effect on normal cells. human tnf was purified to apparent homogeneity as a 17.3-kilodalton protein from hl-60 leukemia cells and showed cytotoxic and cytostatic activities against various human tumor cell lines. the amino acid sequence was determined for the amino terminal end of the purified protein, and oligodeoxyribonucleotide probes were synthesized on the basis of this sequence. complementary dna ... | 1985 | 3856324 |
| [enterotoxin plasmids transmission of escherichia coli]. | | 1985 | 3884293 |
| [model of acute appendicitis reproduced by using the shwartzman phenomenon]. | | 1985 | 3885145 |
| computed tomographic evaluation of an experimental model for pyogenic liver abscesses. | computed tomography (ct) was used to evaluate 15 rabbits with experimentally induced liver abscesses. the animals were examined both before and after intravenous contrast injection. after sacrificing the animals, postfreeze ct scans were made to mark the abdomen for 1-cm thick whole body sections for correlating the gross pathology with the results of the ct scans. ct detected 15 abscesses in 13 of the 14 rabbits with true positive lesions. ten abscesses less than 1.4 cm in diameter were not det ... | 1985 | 3886589 |
| cloning of the gene encoding the hydrogenase from desulfovibrio vulgaris (hildenborough) and determination of the nh2-terminal sequence. | the gene encoding the hydrogenase from desulfovibrio vulgaris (hildenborough) has been cloned in escherichia coli. d. vulgaris dna was digested with the restriction endonucleases ecori and sali and ligated into the vector puc9 [vieira, j. & messing, j. (1982) gene 19, 259-268], which had been cut with these same enzymes. approximately 9000 recombinant clones were obtained by transformation of e. coli jm 101 followed by growth on rich plates with ampicillin for selection and isopropyl-beta-d-thio ... | 1985 | 3888620 |
| aztreonam therapy in experimental meningitis due to haemophilus influenzae type b and escherichia coli k1. | the penetration of aztreonam into cerebrospinal fluid was 7 to 15% and 9 to 25%, respectively, in experimental haemophilus influenzae type b and escherichia coli k1 meningitis. aztreonam was effective in reducing the number of organisms in cerebrospinal fluid after single-dose and continuous infusion administration, and the median bactericidal titers in cerebrospinal fluid were 1:32 against both meningeal pathogens. | 1985 | 3890733 |