| [haemolitic escherichia coli in dropsy disease in piglets]. | | 1965 | 5334419 |
| response of the rabbit ileal loop to cell-free products from escherichia coli enteropathogenic for swine. | | 1970 | 4905648 |
| [biosynthesis and structure of pyruvate dehydrogenase]. | | 1970 | 4911892 |
| [occurrence of an oxygen saturation deficit following experimental administration of coli endotoxin to pigs]. | | 1969 | 4912197 |
| antibiotic-containing enrichment media for certain escherichia coli strains of porcine origin. | | 1968 | 4918219 |
| the presence of n2-fixing bacteria in the intestines of man and animals. | | 1970 | 4922762 |
| comparative assay of endotoxins by oral and parenteral administration. | | 1966 | 5341726 |
| [messenger rna of hemoglobin]. | | 1970 | 4927419 |
| antibiotic sensitivity patterns and r factors in domestic and wild animals. | | 1971 | 4927560 |
| gamma-aminobutyric acid: quantity, production, and metabolism in the brain of pigs. | | 1971 | 4937611 |
| isolation and partial characterization of histidine decarboxylase and edema disease-inducing toxin of escherichia coli strain o139k82h1. | | 1971 | 4940147 |
| characteristics of antimicrobial resistance of escherichia coli from animals: relationship to veterinary and management uses of antimicrobial agents. | five-hundred fifty-five (555) isolates of escherichia coli were obtained from fecal specimens of a representative number of animals from five farms in the united states. antibiotic exposure of the selected herds was determined by an epidemiological survey of these farms. the incidence of multiple resistance in the e. coli isolates was higher in herds exposed to continuous feeding of antimicrobial agents (84.8%) than in a herd not receiving antimicrobials (15.7%). the most common resistance confi ... | 1971 | 4943278 |
| continuous non-therapeutic use of antibacterial drugs in feed and drug resistance of the gram-negative enteric florae of food-producing animals. | fecal samples from illinois farm animals and montana range cattle were examined for the frequency of gram-negative enteric organisms resistant to oxytetracycline, dihydrostreptomycin, or ampicillin. the illinois farm animals were continually fed rations containing various antibacterial drugs. the montana range cattle had minimal exposure to antibacterial drugs. the majority of samples from illinois farm animals demonstrated 10% or more antibiotic-resistant organisms. in contrast, resistant organ ... | 1974 | 4615630 |
| [mesentary air vesicles. an interesting condition in veterinary examination of meat]. | | 1973 | 4618932 |
| enteropathogenicity: recent developments. | recent studies have added important new information to our understanding of the pathogenesis and ethiology of diarrheal disease. vibrio cholerae produces a heat-labile enterotoxin, affecting cyclic amp. a very similar heat-labile enterotoxin is produced also by certain strains of escherichia coli, as well as by citrobacter, klebsiella, and aeromonas. e. coli may also produce a heat-stable enterotoxin, stimulating guanylate cyclase activity. in order to produce the pathologic effects, e. coli fir ... | 1982 | 6126611 |
| properties of the enterotoxic component in escherichia coli enteropathogenic for swine. | the enterotoxic component in sterile syncase broth filtrates of escherichia coli strains 340 (o9:k.:nm) and p307 (o8:k87,88a,b:h19) was studied. the enterotoxic activity in both strains was retained by an ultrafiltration membrane with a molecular weight retention of 100,000 (xm-100a) and eluted from a sephadex g-200 column in the void volume. the enterotoxic activity in strain 340 was resistant to heating at 75 c for 30 min, but the activity in strain p307 was destroyed by heating at 60 c for 30 ... | 1973 | 4633289 |
| effect of coli endotoxin on some blood consituents of the pig. | | 1966 | 4959053 |
| ribosomal rna homologies among distantly related organisms. | the similarity in base sequences of ribosomal rna (rrna) in widely divergent organisms was compared. hybrids were prepared between escherichia coli and pea rrna on the one hand and the dna of several organisms on the other. the varying thermal stabilities of these hybrids were taken as evidence for a hierarchy of rrna relatedness among some higher plants, and for phylogenetic relationships among some species of bacteria, protozoa, fungi, plants, and animals. the large rrna molecule appears to ha ... | 1970 | 4984236 |
| serological changes in immunoglobulins igg, iga and igm and escherichia coli antibodies in the young pig. | | 1970 | 4986977 |
| [treatment of colibacilloses in newborn piglets using combinations of furazolidone and neomycin or chloramphenicol]. | | 1971 | 5006062 |
| [studies on e. coli infections in young swine (colienteritis)]. | | 1965 | 5320620 |
| the use of gallimycin injectable, erythromycin & furacin water mix in a problem herd of spf sows. | | 1966 | 5321682 |
| comparison of heat-labile enterotoxins from porcine and human strains of escherichia coli. | heat-labile enterotoxins (lts) from porcine ewd299) and human (h74-114) enterotoxigenic strains of escherichia coli were isolated by a single-step galactose elution procedure. although both strains had similar amounts of lt in their whole-cell lysates, h74-114 yielded a smaller quantity of purified lt than did ewd299. immunodiffusion studies with specific antisera revealed that although the two lts shared major antigenic determinants each also had unique antigens. both also had shared and unique ... | 1982 | 6176542 |
| comparison of the bactericidal activity of different vertebrate sera. | schwab, g. e. (university of adelaide, adelaide, south australia), and p. r. reeves. comparison of the bactericidal activity of different vertebrate sera. j. bacteriol. 91:106-112. 1966.-the bactericidal activity for gram-negative bacteria of normal sera from eight species of vertebrates was investigated to compare complement-mediated killing in sera from animals representing various classes of vertebrates. although all the sera were bactericidal, there was considerable variation in the range of ... | 1966 | 5323292 |
| increase of the effect of histamine by e. coli endotoxin on the smooth muscle. | | 1965 | 5328605 |
| amino acid incorporation and aminoacyl transfer in a wheat embryo system. | | 1966 | 5333559 |
| some effects of local anaesthetics on cell metabolism. | | 1966 | 5336557 |
| influence of diet and bacteria on small intestinal morphology, with special reference to early weaning and escherichia coli. studies with germfree and gnotobiotic pigs. | | 1966 | 5338249 |
| serologic types of escherichia coli isolated from certain pigs with enteric disorders. | | 1966 | 5338644 |
| use of radioactive isotopes in studies on phagocytosis in vitro. | | 1966 | 5339582 |
| comparative studies on the toxicity of escherichia coli lipopolysaccharide endotoxin in various animal species. | | 1966 | 5339644 |
| transfer of escherichia coli antibodies from sow to piglet. | | 1971 | 4944320 |
| effects of oxaloacetate and l-glutamate on glyceraldehyde 3-phosphate inhibition of aspartate aminotransferase isozymes as measured by a 2oxoglutarate dehydrogenase coupled assay. | | 1971 | 4945269 |
| receptor analogues and anti-pili antibodies as inhibitors of bacterial attachment in vivo and in vitro. | | 1983 | 6135384 |
| effect of bambermycins, in vitro, on plasmid-mediated antimicrobial resistance. | the potential of bambermycins (a growth-promoting antimicrobial approved for turkeys, broilers, and swine) to overcome or control plasmid-mediated antimicrobial resistance was determined in a series of in vitro experiments. four possible modes of action of bambermycins were studied: synergistic effect with 12 other antimicrobials, elimination of resistance (r) plasmids from escherichia coli, selective killing or inhibition of e coli carrying r plasmids, and inhibition of r plasmid transfer. bamb ... | 1984 | 6151815 |
| possible extracellular and intracellular bactericidal actions of mouse complement. | | 1967 | 5342410 |
| role of differential air pressure zones in the control of aerosols in a large animal isolation facility. | the uncontrolled transmission of hog cholera in a large animal isolation facility, designed to control the movement of aerosols within and between individual wings of a multiunit building, indicated the need for a critical study of aerosol behavior under existing conditions of operation. studies with aerosols of escherichia coli b t3 bacteriophage (t3 coliphage) conclusively demonstrated the impossibility of obtaining the desired control by means of a "static" air balance relationship between ad ... | 1966 | 5951332 |
| behaviour of campylobacter sputorum subspecies mucosalis in gnotobiotic pigs. | gnotobiotic pigs were dosed orally with campylobacter sputorum subspecies mucosalis, either alone, or combined with rotavirus or non-pathogenic escherichia coli and streptococcus bovis to study the behaviour of c s mucosalis in defined conditions, to assess intracellular parasitism of enterocytes by c s mucosalis, and if possible to establish an experimental model of porcine intestinal adenomatosis. c s mucosalis colonised the gut of gnotobiotic pigs, persisting for up to 47 days after infection ... | 1984 | 6087431 |
| [the development of antibiotic resistance of coliform bacteria in connection with the nutritional use of nourseothricin in swine]. | | 1984 | 6099110 |
| adhesion of enterotoxigenic escherichia coli in humans and animals. | enterotoxigenic escherichia coli (etec), an important cause of diarrhoea in humans and animal, require accessory virulence properties in addition to enterotoxin to manifest virulence. several classes of pili (hair-like protein surface organelles) promote adhesion of etec to small intestinal mucosa. antibody directed against adhesion pili interferes with colonization of the small intestine and prevents disease. this paper reviews studies with purified k88, k99 and 987 type pili used as parenteral ... | 1981 | 6114818 |
| pilus production, hemagglutination, and adhesion by porcine strains of enterotoxigenic escherichia coli lacking k88, k99, and 987p antigens. | three strains of enterotoxigenic escherichia coli which adhered, colonized intensively, and caused disease in pig intestine, but which did not produce pili of the k88, k99, or 987p antigen types were designated 3p(-) etec. the 3p(-) etec caused mannose-resistant hemagglutination, adhered to porcine intestinal epithelial cells in vitro, and produced pili. however, most bacteria taken directly from the intestine of pigs infected with 3p(-) etec appeared to be nonpiliated. two preparations were iso ... | 1982 | 6119295 |
| virulence of escherichia coli in ascending urinary-tract infection in mice. | the virulence of escherichia coli strains in ascending urinary-tract infection was studied in mice drinking a 5% glucose solution; factors determining the virulence were examined. of 33 strains, 8 (group i) infected the bladder and kidney, 10 (group ii) infected only the bladder, while the remaining 15 strains (group iii) did not cause infection. the adherence of group-i and group-ii strains to bladder epithelial cells in vitro was inhibited by d-mannose. in group iii, 13 strains barely adhered ... | 1982 | 6126593 |
| aspects on structure and function of pili on uropathogenic escherichia coli. | | 1983 | 6131425 |
| density and localization of p-fimbriae-specific receptors on mammalian cells: fluorescence-activated cell analysis. | more than 95% of acute non-obstructive pyelonephritis cases in children are caused by p-fimbriated escherichia coli. uroepithelial cells from individuals prone to urinary tract infections bind such bacteria more avidly than do cells from healthy controls. a high density of receptors for p-fimbriae on cells of the urinary tract and kidney may thus be an important host factor in the acquisition of upper urinary tract infections. in this communication we are describing methods to determine the dens ... | 1983 | 6132882 |
| effect of escherichia coli heat-stable enterotoxin, cholera toxin and theophylline on ion transport in porcine colon. | 1. the effect of heat-stable enterotoxin (st) of escherichia coli, cholera toxin (ct), and theophylline (a phosphodiesterase inhibitor) on ion and water transport was studied with an in vivo isolated loop system of the pig colon.2. all three agents abolished net na absorption as a result of a decrease in the lumen to blood na flux alone. with all three agents, net cl absorption was reduced, but not abolished, and net hco(3) secretion was elicited. luminal p(co2) was reduced with ct and theophyll ... | 1981 | 6275079 |
| multi-adhesin vaccines for the protection of the neonatal piglet against "e. coli" infections. | it has been shown that the initial stages of enteric infection with e. coli organisms involve attachment of the organism to the intestinal villi. attachment is mediated by specific protein adhesins on the surface of the organism such as k88ab, k88ac, k99 and 987p (1). purified preparations of each of these adhesins have been used for the vaccination of pregnant gilts and been shown to confer passive protection to their piglets against homologous but not heterologous challenge with e. coli (2, 3) ... | 1983 | 6135633 |
| the production of f41 fimbriae by piglet strains of enterotoxigenic escherichia coli that lack k88, k99 and 987p fimbriae. | the enterotoxigenic escherichia coli strains 1676, 1706, 1751 and kec96a, which do not produce fimbrial adhesive antigens of the k88, k99 or 987p antigen type reacted both in vitro and in vivo with antiserum to f41 fimbriae in an indirect immunofluorescent antibody technique. antiserum used to demonstrate material b, an adhesive antigen thought to mediate the adhesive and mannose-resistant (mr) haemagglutinating properties of e. coli strains 1676, 1706 and 1751, reacted in vitro with an f41+ str ... | 1983 | 6138394 |
| recovery of intestinal membrane binding sites for k88 e. coli from pig mucosal organ cultures. | putative receptors for k88 + e. coli from piglet intestinal epithelium were released into the organ culture medium and were demonstrated by direct binding with k88 + e. coli through the utilization of an in vitro binding procedure or by immunoprecipitation with k88 antigen. incorporation of 14c-glucosamine by newborn to day old and 3-week to 6-week old piglet jejunal and ileal mucosa, in organ culture, occurred throughout the 24 hr culture period. uptake in both age groups and both areas of the ... | 1984 | 6146093 |
| drug resistance and r plasmids in escherichia coli isolated from laboratory animals. | | 1983 | 6345875 |
| acid-base and electrolyte changes in 1-3 days old piglets infected with enteropathogenic escherichia coli and in spontaneous cases of piglet diarrhoea. | | 1983 | 6346838 |
| microbiological studies on hamburgers. | one hundred and eighty-two raw, 112 pre-cooked and 750 cooked hamburgers composed mainly of beef or beef and pork were subjected to microbiological examination.raw hamburgers gave total bacterial counts from 10(6) to 10(8) per g, counts of enterobacteriaceae from 10(4) to 10(6) per g, of escherichia coli from 10(3) to 10(5), of group d streptococci from 10(2) to 10(4), of staphylococcus aureus from 3 to 10(2) and of clostridium perfringens less than 10 bacteria per g. of the samples, 32% contain ... | 1982 | 6276464 |
| cloning, partial sequencing, and expression of glyceraldehyde-3-phosphate dehydrogenase gene in chick embryonic heart muscle cells. | two recombinant plasmids containing structural gene sequences of chick embryonic heart glyceraldehyde-3-phosphate dehydrogenase (gap dehydrogenase) were constructed and characterized. the plasmids pgap 30 and pgap 36 have inserts of 1200 and 950 base pairs, respectively. the identity of the clones was established by hybrid-arrested and hybrid-selection translation assays, and by immunoprecipitation of hybrid-selected translation product with gap dehydrogenase antiserum. hybridization of labeled ... | 1982 | 6179937 |
| immunological and physicochemical characterization of heat-labile enterotoxins isolated from two strains of escherichia coli. | heat-labile enterotoxins from escherichia coli strains of human and porcine origins had identical subunit composition, arrangement, and size in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. immunological differences recently described by others were shown to reside exclusively in the b subunits. | 1982 | 6183221 |
| [cloning and expression of the genetic determinants encoding for the serological variants of the adhesive factor k 88 carried by enterotoxinogenic escherichia coli strains of porcine origin]. | the existence of homologous sequences on plasmids carried by 3 enterotoxinogenic strains of escherichia coli of porcine origin, and encoding for adhesive factors of different antigenic specificities: k 88 ab, k 88 ac, k 88 ad; has been demonstrated by dna-dna hybridization. the use as probe of a dna fragment carrying a piece of structural genes of the adhesive factor k 88 ab taken out of a recombinant plasmid expressing this factor, has allowed us to localize the genetic determinants which encod ... | 1982 | 6186346 |
| novel mucosal anti-microbial functions interfering with the plasmid-mediated virulence determinants of adherence and drug resistance. | mucosal antibodies in vivo and in vitro interfere with the stability of plasmids coding for important virulence determinants in porcine enteropathogenic e. coli (eec), such as the adhesion determinants k88ab and k88ac. the effector antibody is not directed against k88 antigens and is not serotype specific, but an antigen common to k88+ strains is implicated. further lack of pathogen specificity is exemplified by antibody elimination of the more recently discovered k88ad plasmid. antibodies that ... | 1983 | 6191616 |
| intraspecific interactions between escherichia coli strains in human newborns and in gnotobiotic mice and piglets. | a plasmid-free human escherichia coli strain emo, was inoculated to human newborns within two hours of life. it became established in the feces at a high population level. ampicillin and tetracycline resistant e. coli strains disappeared or decreased to subdominant level in the inoculated newborns whereas they remained at a high level in the noninoculated newborns. strain emo was also shown to exert a barrier effect against a porcine plasmid-bearing enterotoxigenic ent+ k88- e. coli strain in gn ... | 1983 | 6361853 |
| immunopathogenesis of chronic pyelonephritis. | immunopathologic responses to urinary tamm-horsfall protein in the development of chronic pyelonephritis were examined by four different approaches. first, in a rabbit model, tubulointerstitial nephritis developed in 64 of 102 rabbits injected intravenously with urine or rabbit tamm-horsfall protein as compared with only one of 17 rabbits in two control groups. circulating cytotoxic lymphocytes plus immunoglobulin g (igg) antibodies against tamm-horsfall protein were found in 51 percent of chall ... | 1983 | 6192715 |
| use of immunofluorescence, gram's staining, histologic examination, and seroagglutination in the diagnosis of porcine colibacillosis. | an indirect fluorescent antibody (ifa) test of ileal impression smears for k88, k99, and 987p pilus antigens was compared with histologic examination and seroagglutination of escherichia coli isolates for efficacy in determining colibacillosis in pigs. histologic examination appeared to be more effective than the ifa test in revealing colonization of the ileum by bacteria. however, histologic examination revealed little about the nature of the colonizing bacteria. correlation between bacterial a ... | 1983 | 6195944 |
| antisecretory effects of berberine with morphine, clonidine, l-phenylephrine, yohimbine or neostigmine in pig jejunum. | the effects of berberine alone or in combination with morphine, clonidine, l-phenylephrine or yohimbine were compared in escherichia coli heat-stable enterotoxin (st)-exposed ligated jejunal loops in 2 week old pigs. in addition, net water and electrolyte fluxes in normal jejunal loops were measured in the presence of neostigmine, morphine, clonidine, l-phenylephrine or yohimbine alone or in combination with berberine. berberine, morphine, clonidine and l-phenylephrine each reduced the net secre ... | 1983 | 6363101 |
| role of the capsule and the o antigen in resistance of o18:k1 escherichia coli to complement-mediated killing. | epidemiological data show that o18:k1 escherichia coli is a common cause of neonatal bacteremia and meningitis. these bacteria were capable of multiplying in the bloodstream of newborn rats and were resistant to the bactericidal effects of complement in the absence of specific antibodies. the roles played by the o antigen and the k antigen in complement resistance were analyzed by comparing the bactericidal effects of normal sera and of sera deficient in various complement components or in immun ... | 1983 | 6196296 |
| o-antigens, k-antigens and production of enterotoxin in escherichia coli-strains isolated from litter mate piglets with neonatal diarrhea. | | 1983 | 6197871 |
| factors contributing to postweaning diarrhoea in a large intensive piggery. | some aspects of postweaning diarrhoea (pwd) in a piggery during the first week after early weaning were investigated. a haemolytic enterotoxigenic strain of e. coli (o149:k88:h10) was regularly recovered from piglets with pwd while rotavirus was demonstrated on a number of occasions. prior to weaning piglets were either free of, or shed very few, haemolytic e. coli in their faeces. however, all piglets were excreting haemolytic e. coli between 5 and 7 days after weaning. the role of rotavirus in ... | 1980 | 6252883 |
| inactivation of microbial pyridoxal kinase by pyridoxal. | pyridoxal kinase from escherichia coli and bakers' yeast was inactivated by pyridoxal while the enzyme from rat and pig brain was not. the inactivation of the enzyme purified from e. coli was reversible and was rendered irreversible by the reduction with nabh4. this finding as well as a similar inactivation by 5'-deoxypyridoxal but not by 4'-deoxypyridoxine suggested that the inactivation was due to schiff base formation. the suggestion was confirmed by the incorporation of tritium label into th ... | 1981 | 6289642 |
| biological activities and spectroscopic properties of chromophoric and fluorescent analogs of adenine nucleoside and nucleotides, 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) adenosine derivatives. | the ribose-modified chromophoric and fluorescent analog of atp 2',3'-o-(2,4,6-trinitrocyclohexadienylidene) adenosine 5'-triphosphate (tnp-atp) has been synthesized previously (hiratsuka, t., and uchida, k. (1973) biochim. biophys. acta 320, 635-647 and hiratsuka, t. (1976) biochim. biophys. acta 453, 293-297). in the present study, four tnp-derivatives of atp, adp, amp and adenosine were synthesized and compared for several chemical, spectral and enzymatic properties. their visible absorption a ... | 1982 | 6295507 |
| study of several factors affecting the agglutinating activity of k99-positive escherichia coli strains. | the effect of several factors on escherichia coli k99-plasmid associated agglutination has been studied. the results obtained indicate that escherichia coli 637 (k99+) mediated red blood cell agglutination is unspecific although the agglutination titres for several erythrocyte species are significantly different. the agglutination is highly stable (at least with sheep red blood cells) to changes in temperature (from 4 degrees c to 37 degrees c), to changes in ph (from 5 to 9) and to the presence ... | 1983 | 6371953 |
| neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks. | to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ... | 1983 | 6299483 |
| [nuclear translocation and effect of camp-dependent protein kinase on transcription]. | the influence of camp-dependent pig brain protein kinase and its subunits on transcription in vitro was studied. the increase in the template activity of chromatin isolated from the nuclei after treatment with the catalytic subunit was observed. the regulatory subunit of protein kinase was found to increase the number of binding sites for rna polymerase on chromatin. the camp-dependent pig brain protein kinase was found to phosphorylate the sigma-factor of escherichia coli rna polymerase. this p ... | 1980 | 6260240 |
| determination of a specific receptor for formyl-methionyl-leucyl-phenylalanine on th pulmonary alveolar macrophage and its relationship to chemotaxis and superoxide production. | 3h-fmlp, a chemotactic peptide that resembles escherichia coli chemotactic factor, is chemotactic for pam, binds specifically to a site on the cell, and induces the generation of superoxide radicals by the cell. scatchard analysis revealed an equilibrium dissociation constant at 26 degrees c of 1.45 x 10(-8)m and the presence of 1.7 , 10(5) receptors per cell. binding was not inhibited by a partially purified c5a preparation or by the neutrophil-derived ccf but was inhibited by various n-formyla ... | 1981 | 6260878 |
| bacterial cell wall components decrease the number of guinea-pig lung beta-adrenoceptors. | infections of the deeper respiratory airways can contribute to the progression of chronic asthmatic bronchitis. in the present report a number of microorganisms affecting the number of beta-adrenoceptors in guinea-pig lung homogenates are described. haemophilus influenzae, streptococcus pneumoniae, bordetella pertussis and escherichia coli o111b4 induced a significant decrease of the number of beta-adrenoceptors (by approximately 20%). staphylococcus aureus, influenza a virus and escherichia col ... | 1983 | 6301848 |
| relatedness of chloramphenicol resistance plasmids in epidemiologically unrelated strains of pathogenic escherichia coli from man and animals. | i have examined 20 plasmids conferring chloramphenicol resistance (cm) in multiresistant strains of escherichia coli pathogenic for man and piglets. in denmark, one plasmid family, exemplar phg33, is responsible for all chloramphenicol resistance in serotypes of e. coli found in diseased piglets. a closely related plasmid, phg50, was identified in an enteropathogenic e. coli (epec) strain from an infant. the isolate was epidemiologically unrelated to the piglet isolates. the molecular relatednes ... | 1983 | 6302257 |
| interactions between entamoeba histolytica, bacteria and intestinal cells. | axenically grown pathogenic and non-pathogenic isolates of entamoeba histolytica have been shown to adhere to mammalian epithelial cells and bacteria by virtue of carbohydrate-binding proteins present on their cell surfaces. the interaction of amoeba isolates of low pathogenicity with a variety of gram-negative bacteria, mainly escherichia coli strains which are readily ingested by the amoebae after relatively short periods, significantly increased the ability of the trophozoites to: (a) destroy ... | 1983 | 6315320 |
| ferritin: protection of enzymatic activity against the inhibition by divalent metal ions in vitro. | ferritin binds a large quantity of be2+ (price d.j. and joshi, j.g. j. biol. chem. 258 (1983) 10873) as well as other divalent metal ions. therefore the ability of this protein to protect enzymes against or reverse the inhibition by metal ions was studied. evidence presented here shows that the inhibition by be2+ of the enzymes na+k+atpase, alkaline phosphatase and phosphoglucomutase is reversed by ferritin. be2+ can be transferred reversibly between phosphoglucomutase and ferritin depending upo ... | 1984 | 6330934 |
| differential toxicity of inhaled gram-negative bacteria. | the toxicity by inhalation of various gram-negative bacteria, isolated from settings associated with inhalation disease, was studied by a variety of means. these microorganisms were not equally toxic. citrobacter freundii aerosol challenges of rabbits provoked significant (up to fivefold) increases in plasma haptoglobin 24 to 48 h after inhalation. other strains tested failed to provoke such statistically consistent increases. measurements of c-reactive protein in these same animals did not lead ... | 1983 | 6339400 |
| an in vitro system to study interactions between bacteria and epithelial cells at the molecular level. | this paper describes an experimental system to study interactions between porcine enterotoxigenic escherichia coli (etec) and porcine intestinal epithelial cells in vitro at the molecular level. radiolabelled bacteria or bacterial membrane fractions were incubated with brush borders prepared from purified epithelial cells, which were then washed repeatedly. the bacterial components removed by washing or retained by the brush borders were analysed to determine their composition and source. for th ... | 1983 | 6339675 |
| gastric mucosal blood flow and escherichia coli bacteremia. | the specific changes in gastric blood flow during sepsis are controversial. previous investigations of intragastric blood flow using endotoxin models revealed decreased total gastric blood flow and decreased gastric mucosal blood flow. endotoxin models are now thought to be inadequate due to the accompanying depression of the systemic circulation. more recently, increased gastric blood flow has been demonstrated in a septic hindlimb model. to further elucidate the changes in intragastric blood f ... | 1983 | 6340545 |
| partial purification and characterization of an escherichia coli toxic factor that induces morphological cell alterations. | a factor produced by several strains of escherichia coli isolated from enteritis-affected children has been shown to produce both a necrotizing effect on rabbit skin and striking morphological alterations on cho, vero, and hela cells. the same strains were found to have hemolytic activity on sheep erythrocytes. the toxic, cell-altering factor was demonstrated to be different from both heat-labile and heat-stable enterotoxins and from vero toxin. the main effect induced by the isolated factor on ... | 1983 | 6341235 |
| from the national institute of allergy and infectious diseases. summary of a workshop on enteropathogenic escherichia coli. | | 1983 | 6343525 |
| vaccination of piglets against post-weaning "e. coli" enterotoxicosis. | e. coli enterotoxicosis is one of the main causes of neonatal diarrhea in piglets. for their protection against this disease a vaccine has been prepared based on k88 and lt. pregnant sows were vaccinated, thereby transferring passive immunity to their offspring through colostrum and milk. protection was obtained in terms of decrease in mortality, occurrence of diarrhea and excretion of enteropathogenic bacteria. piglets from non-immune sows could be immunized shortly after birth. however, piglet ... | 1983 | 6347759 |
| enterotoxigenicity of bovine and porcine escherichia coli of o groups 8, 9, 20, 64, 101, and x46. | | 1983 | 6349437 |
| plasmid-mediated adhesion in enteropathogenic escherichia coli. | a survey of classical serotype enteropathogenic escherichia coli has been made with respect to their plasmid profile and ability to adhere to hep-2 cells. thirty-one of the 32 strains examined contained a 50-70 md plasmid, and many exhibited hep-2 adherence. strain e2348 (0127:h6), which causes diarrhea in volunteers and is hep-2-adhesive, was chosen for further study. the large 55 md plasmid in e2348, pmar2, has been marked with a transposon coding for ampicillin resistance. e2348 that has been ... | 1983 | 6352891 |
| the effect of antibiotics on mannose-resistant haemagglutination by k88- and k99-positive escherichia coli strains. | five e. coli strains carrying k99 antigen isolated from the intestines of calves which had succumbed to diarrhoea and six k88-positive strains isolated from fatal cases of diarrhoea in piglets were examined for their mannose-resistant haemagglutination (mrha) capacity against pig erythrocytes. the bovine strains showed a geometric mean mrha-titre of 1/18 and the porcine strains one of 1/45. similar experiments were carried out after addition of the following antibiotics in doubling dilutions: am ... | 1983 | 6355504 |
| epizootiological studies on r plasmid with carbadox resistance. | | 1983 | 6355590 |
| the clinical manifestation and pathogenesis of enteritis associated with rotavirus and enterotoxigenic escherichia coli infections in domestic animals. | rotavirus and enterotoxigenic escherichia coli (etec) are enteropathogens each capable of inducing diarrhoea in some animal species and man. unstressed young animals develop an age-related resistance to infection with either rotavirus or etec which differs for each animal species. the effects of experimental infection of calves, lambs, foals and piglets with rotavirus and etec given either alone or in combination, have been examined. in general, dual infections tended to lengthen the period of a ... | 1983 | 6361857 |
| [passive immunoprophylaxis against coli dysentery of suckling pigs by vaccination of sows with attenuated escherichia coli mutants. 1. clinical and laboratory diagnostic results of the combined use of k88- and k99-antigen-containing strains derived from escherichia coli mutants]. | | 1983 | 6362603 |
| role of peripheral pooling in porcine escherichia coli sepsis. | in anesthesized pigs the effects of e. coli (2 x 10(8)/kg) on hemodynamics and red cell distribution were studied. after injection of 99m-tc red cells (15 mci), regional radioactivity was followed during 3 hours. gated bloodpool studies were performed to measure end-diastolic volumes (edv). escherichia coli e. coli was infused in 14 pigs, while 7 animals served as controls. e. coli resulted in an early increase in pulmonary arterial pressure. systemic arterial pressure decreased gradually, while ... | 1984 | 6368039 |
| heme-iron and ecology of escherichia coli within the porcine gut. | growth of escherichia coli was followed in sow whey by a turbidometric technique. heme-iron clearly promoted bacterial growth, therefore free heme would be dangerous in small intestine by enhancing bacterial growth: duodenal brush border membrane (bbm) was seen to bind heme-iron, thus abolishing the growth promoting effect of this form of iron. scatchard analysis of heme-binding onto bbm showed that heme became bound by a specific mechanism (kd approximately equal to 10(-7) m) as well as non-spe ... | 1983 | 6368116 |
| effect of bacterial immunization on experimental reflux nephropathy. | this study was designed to test the hypothesis that young animals with vesicoureteral reflux might be more vulnerable to renal parenchymal infection by bacteria to which they had not been previously exposed. forty-four crossbred male piglets had surgical induction of vesicoureteral reflux at 2 weeks of age and introduction of urinary tract infection at 6 weeks. they were sacrificed at 12 weeks of age. between the ages of 2 and 6 weeks, 22 piglets received subcutaneous injections of formalin-kill ... | 1984 | 6368870 |
| [dynamics of antibiotic resistance of strains of escherichia coli isolated from feces of weaned piglets (the case of the lwanika- lubumbashi (zaire) farm]. | | 1983 | 6369447 |
| relationship of iron administration to susceptibility of newborn pigs to enterotoxic colibacillosis. | to determine whether supplemental iron (fe) administration to newborn pigs reared in concrete pens not only prevents anemia, but renders the pigs more susceptible to escherichia coli-induced diarrheal disease, pigs were given a large or a small dose of fe im or orally before or after challenge exposure with e coli. the controls were challenge-exposed pigs not given fe and pigs not challenge exposed (fe-treated and nontreated groups). although the mortality of the pigs challenge exposed with e co ... | 1984 | 6370051 |
| enteric diseases in animals caused by escherichia coli: their control and prevention. | | 1984 | 6373433 |
| drug resistance and conjugative r plasmids in fecal escherichia coli strains isolated from healthy younger animals (chickens, piglets, calves) and children. | a total of 11,777 escherichia coli strains were isolated from 90 chickens, 103 piglets, 96 calves, and 104 children in 1979 in gunma prefecture and tested for drug resistance and the presence of conjugative r plasmids. the percentages of individuals that excreted drug-resistant strains were: chickens, 100%; piglets, 99%; calves, 100%; and children, 64%. the frequency of isolation of drug-resistant strains among the total isolates was: chickens, 98%; piglets, 93%; calves, 94%; and children, 41%. ... | 1983 | 6374389 |
| prevention of colibacillosis in neonatal swine with a 4-pilus e coli bacterin. | of 12 pregnant swine (vaccinates) given a 4-pilus enterotoxigenic e coli (etec) bacterin (k88, k99, 987p, f41), all developed comparable or significantly higher serum and colostral antibody levels than those of 8 pregnant swine (controls) given a 3-pilus etec bacterin (k88, k99, 987p). when piglets were challenged with an etec strain bearing the f41 antigen, those from vaccinates had significantly lower mortality, less scours, less severe clinical signs and better weight gain than those from con ... | 1984 | 6374422 |
| the reaction of pyridoxal phosphate with holoenzymes in the pyridoxamine phosphate form. | | 1984 | 6374674 |
| [immunogenesis of 2 live vaccines against swine colibacillosis]. | comparative testing was carried out on a farm with 2 live vaccines against coli bacteriosis . studied were blood and milk sera taken from test and control pigs and blood sera from pigs born by them. both the test and control groups were clinically followed up with recording the morbidity and mortality indices. it was found that the titer of the k88 antibodies rose after vaccination with the two vaccines and retained its level up to the 30th day after farrowing. the titer of the o antibodies rema ... | 1984 | 6375113 |
| [properties of e. coli isolated from pigs]. | a total of 127 strains of escherichia coli were isolated from the small intestine, mesenterial lymph nodes, and parenchymal organs from dead pigs or pigs that were killed for diagnostic purpose as well as from rectal swab samples taken from diseased or clinically normal animals. all were studied with regard to haemolytic properties via biochemical and serologic methods along for sensitivity to antibiotics. it was found that the isolated strains manifested characteristic biochemical behaviour (79 ... | 1984 | 6375116 |
| dietary regimen, rotavirus, and hemolytic enteropathogenic escherichia coli in weanling diarrhea of pigs. | previously, we induced weanling diarrhea in piglets by infecting them first with rotavirus followed by a hemolytic enteropathogenic escherichia coli. we postulated that rotavirus, by damaging the epithelium of the small intestines, produced an entero-environment which favored the selection and growth of the enteropathogenic e. coli. furthermore, diet might affect the entero-environment and influence the course of the disease. to test this, newly weaned 3-week-old piglets were assigned to one of ... | 1983 | 6375527 |
| the role of dietary antigen in the aetiology of post weaning diarrhoea. | experiments were undertaken to investigate whether a hypersensitivity response to dietary antigen might be a predisposing factor in the aetiology of post weaning diarrhoea. the results indicated that: 1. very small amounts of food given to baby pigs before weaning significantly increased the severity and accelerated the onset of the disease; 2. post weaning diarrhoea was dependent upon the presence within the diet of antigenic material; 3. weaning diets containing little antigenic material had l ... | 1983 | 6375529 |
| [trends in drug therapy: electolyte transport in the intestines. the pathophysiology and therapy of enterotoxin-caused diarrheas]. | | 1984 | 6375654 |
| [amino acid composition of the alpha-ketoglutarate dehydrogenase from the breast muscle of the pigeon]. | the amino acid composition of alpha-ketoglutarate dehydrogenase--a component of alpha-ketoglutarate dehydrogenase complex--from the pigeon breast muscle has been determined. a significant similarity of the enzyme amino acid composition isolated from systematically remote species has been revealed by comparison of the data obtained with the literary one concerning the alpha-ketoglutarate dehydrogenase from escherichia coli and pig heart. | 1984 | 6375738 |
| antimicrobial resistance and conjugative r plasmids in escherichia coli strains isolated from animals in peninsular malaysia. | fifteen independent e. coli strains of avian, bovine and porcine origin in peninsular malaysia were tested for antibiotic resistance and conjugative r plasmids. eight (53%) isolates were found to be antibiotic resistant. among them, 37.5% were mono-resistant and 62.5% were resistant to three or more antibiotics, i.e., multi-resistant. all of them were resistant to tc and sensitive to gm and nx. three of the eight antibiotic resistant strains were able to transfer all or part of their resistance ... | 1984 | 6377513 |