| artificial insertion of a dominant gene for resistance to avian leukosis virus into the germ line of the chicken. | this report describes the unique biological properties of a transgenic chicken line that contains a defective avian leukosis virus (alv) proviral insert that we call alv6. chick embryo fibroblasts (cef) containing this insert express subgroup a envelope glycoprotein since they yield focus-forming pseudotype virus when co-cultivated with transformed quail cells expressing envelope-defective bryan high-liter rous sarcoma virus (rsv). in addition, these cells display high interference to subgroup a ... | 1989 | 24232709 |
| incidence of endogenous viral genes in two strains of white leghorn chickens selected for egg production and susceptibility or resistance to marek's disease. | endogenous viral (ev) genes related to the avian leukosis virus were classified in two differentially selected strains of leghorns in order to investigate whether such genes affect production traits. strain k had been selected for resistance to marek's disease (md) and for high egg production and egg weight, whereas strain s had been selected only for md susceptibility. except that founders of strain k included a few commercial birds, both strains were derived from a common genetic base. dna res ... | 1989 | 24232469 |
| development of a taqman-based real-time pcr assay for rapid and specific detection of fowl aviadenovirus serotype 4. | twelve serotypes of fowl aviadenovirus, namely, fadv-(1-8a and 8b-11), have been identified, among which fadv-4 is the aetiologic agent of hepatitis hydropericardium syndrome (hhs) in chickens. outbreaks of hhs have been documented in many countries, causing significant economic losses. real-time pcr methods described so far in the literature cross-detect different serotypes of fadvs. in this study, we aimed to develop a taqman-based real-time pcr assay for the specific detection of fadv-4. a pa ... | 2017 | 28437156 |
| 16s rrna genes illumina sequencing revealed differential cecal microbiome in specific pathogen free chickens infected with different subgroup of avian leukosis viruses. | intestinal flora play important roles in the pathogenisis of many pathogens. this study examined the cecal microbiome of chickens infected with avian leukosis virus (alv) using 16s rrna genes illumina sequencing. one-day-old specific pathogen free chicks were inoculated in the abdomen with subgroup j or k of alv. at 21-day-old, chickens positive for alv viremia were selected and their cecal contents were extracted and examined for the composition of gut microflora by illumina sequencing of the v ... | 2017 | 28757024 |
| complete genome sequencing and characterization revealed a recombinant subgroup b isolate of avian leukosis virus with a subgroup j-like u3 region. | one natural recombinant subgroup b avian leukosis virus (alv) with a subgroup j-like u3 region was isolated from commercial native chickens that experienced disease in 2014 and named gx14ff03. gx14ff03 was isolated by df-1 cell culture and then identified with elisa detection of avian leukosis virus p27 group-specific antigen, the detection of subtype specific pcr, and indirect immunofluorescence assay with alv-b-specific monoclonal antibody. its complete proviral genome was sequenced and compar ... | 2017 | 28718046 |
| full-length genome sequence analysis of four subgroup j avian leukosis virus strains isolated from chickens with clinical hemangioma. | since 2014, cases of hemangioma associated with avian leukosis virus subgroup j (alv-j) have been emerging in commercial chickens in guangxi. in this study, four strains of the subgroup j avian leukosis virus (alv-j), named gx14hg01, gx14hg04, gx14lt07, and gx14zs14, were isolated from chickens with clinical hemangioma in 2014 by df-1 cell culture and then identified with elisa detection of alv group specific antigen p27, the detection of subtype specific pcr and indirect immunofluorescence assa ... | 2017 | 28718045 |
| dynamic co-evolution and interaction of avian leukosis virus genetic variants and host immune responses. | subgroup j avian leukosis virus (alv-j), a typical retrovirus, is characterized of existence of a cloud of diverse variants and considerable genetic diversity. previous studies describing the evolutionary dynamics of alv-j genetic variants mainly focused on the early infection period or few randomly selected clones. here, we inoculated 30 specific-pathogen-free chickens with the same founder alv-j stock of known genetic background. six (three antibody positive and three antibody negative) chicke ... | 2017 | 28694798 |
| discovery of novel long non-coding rnas induced by subgroup j avian leukosis virus infection in chicken. | avian leukosis virus subgroup j (alv-j) is an avian oncogenic retrovirus that has led to severe economic losses in the poultry industry in china in recent decades. here, using high throughput transcriptome sequencing of hd11 and cef cells infected with alv-j, a set of 4804 novel long non-coding transcripts and numerous differentially expressed long non-coding rnas (lncrnas) were identified. we also found that they share relatively shorter transcripts and fewer exon numbers compared to mrna. corr ... | 2017 | 28673822 |
| vertical transmission of avian leukosis virus subgroup j (alv-j) from hens infected through artificial insemination with alv-j infected semen. | avian leukosis virus (alv) is one of the main causes of tumour development within the poultry industry in china. the subgroup j avian leukosis viruses (alv-j), which induce erythroblastosis and myelocytomatosis, have the greatest pathogenicity and transmission ability within this class of viruses. alv can be transmitted both horizontally and vertically; however, the effects of alv infection in chickens-especially roosters-during the propagation, on future generations is not clear. knowing the ro ... | 2017 | 28662658 |
| a novel avian retrovirus associated with lymphocytoma isolated from a local chinese flock induced significantly reduced growth and immune suppression in spf chickens. | avian leukosis viruses (alvs) are associated with neoplasias, immune suppression and reduced performance in chicken flocks. in the present study, a naturally occurring recombinant strain of alv (fj15ht0) was isolated from an infected flock of chinese "hetian" chickens, and was subsequently identified as an exogenous alv by immuno-fluorescence assay (ifa), pcr and following entire proviral dna nucleotide sequencing. this isolate is revealed as a novel recombinant virus, lacking viral oncogenes, w ... | 2017 | 28622858 |
| molecular characterization, expression of chicken tbk1 gene and its effect on irf3 signaling pathway. | traf family member-associated nf-κb activator (tank)-binding kinase1 (tbk1) is a serine-threonine kinase at the crossroads of multiple interferon (ifn)-inducing signaling pathways in innate immunity. the importance of tbk1 in antiviral immunity is well established in mammal models, but in chicken, the molecular characterization and potential function of tbk1 remain unclear. in the present study, the open-reading frame (orf) of chicken tbk1 (chtbk1) was cloned and characterized. the sequencing re ... | 2017 | 28493975 |
| circular rna alterations are involved in resistance to avian leukosis virus subgroup-j-induced tumor formation in chickens. | avian leukosis virus subgroup (alv-j) is an oncogenic neoplasm-inducing retrovirus that causes significant economic losses in the poultry industry. recent studies have demonstrated circular rnas (circrnas) are implicated in pathogenic processes; however, no research has indicated circrnas are involved in resistance to disease. in this study, over 1800 circrnas were detected by circrna sequencing of liver tissues from alv-j-resistant (n = 3) and alv-j-susceptible chickens (n = 3). 32 differential ... | 2017 | 28415618 |
| integrated host and viral transcriptome analyses reveal pathology and inflammatory response mechanisms to alv-j injection in spf chickens. | avian leukosis virus (alv) is detrimental to poultry health and causes substantial economic losses from mortality and decreased performance. because tumorigenesis is a complex mechanism, the regulatory architecture of the immune system is likely to include the added dimensions of modulation by mirnas and long-noncoding rna (lncrna). to characterize the response to alv challenge, we developed a novel methodology that combines four datasets: mrna expression and the associated regulatory factors of ... | 2017 | 28401895 |