| prevalence of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythia in japanese patients with generalized chronic and aggressive periodontitis. | this study aimed to investigate the prevalence and levels of major periodontal pathogens, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythia in subgingival plaque samples of a group of japanese patients with aggressive periodontitis (agp) and chronic periodontitis (cp). a total of 40 patients with clinical diagnosis of agp or cp and 10 periodontally healthy volunteers were subjected to clinical and microbiological analysis. subgingival plaque samples were an ... | 2013 | 23608307 |
| gene expression and phenotypic traits of aggregatibacter actinomycetemcomitans in response to environmental changes. | periodontopathogens experience several challenges in the oral cavity that may influence their transcription profile and resulting phenotype. this study evaluated the effect of environmental changes on phenotype and gene expression in a serotype b aggregatibacter actinomycetemcomitans isolate. | 2013 | 23600969 |
| lipopolysaccharide of aggregatibacter actinomycetemcomitans up-regulates inflammatory cytokines, prostaglandin e2 synthesis and osteoclast formation in interleukin-1 receptor antagonist-deficient mice. | the interleukin (il)-1 receptor antagonist (ra) binds to il-1 receptors and inhibits il-1 activity. however, it is unclear whether the il-1ra plays a protective role in periodontal disease. the purpose of this study was to compare il-1ra knockout (ko) and wild-type (wt) mice in regard to proinflammatory cytokine production, osteoclast formation and bone resorption in response to periodontal bacterial lipopolysaccharide (lps). | 2013 | 23586622 |
| quantitative real-time pcr combined with propidium monoazide for the selective quantification of viable periodontal pathogens in an in vitro subgingival biofilm model. | differentiation of live and dead cells is an important challenge when using molecular diagnosis for microbial identification. this is particularly relevant when bacteria have been exposed to antimicrobial agents. the objective of this study was to test a method using quantitative real-time polymerase chain reaction (qpcr) combined with propidium monoazide (pma), developed for the selective quantification of viable p. gingivalis, a. actinomycetemcomitans, f. nucleatum and total bacteria in an in ... | 2014 | 23581569 |
| cell junction remodeling in gingival tissue exposed to a microbial toxin. | the gingival epithelium plays a key role in protecting the supporting structures of the teeth from bacteria and their products. in ex vivo experiments, we recently showed that the cytolethal distending toxin (cdt) from the periodontal pathogen aggregatibacter actinomycetemcomitans causes extensive damage to gingival tissue. morphological changes included detachment of the keratinized outer layer, distention of spinous and basal cells in the oral epithelium, disruption of rete pegs, and apparent ... | 2013 | 23576426 |
| phenotypic changes in nonfimbriated smooth strains of aggregatibacter actinomycetemcomitans grown in low-humidity solid medium. | aggregatibacter actinomycetemcomitans is the primary etiologic agent of localized aggressive periodontitis. in vitro, it can undergo fimbriated rough to nonfimbriated smooth phenotypic transition, accompanied by an increase in invasive ability and a decrease in adhesive ability. no opposite direction phenotypic transition was reported. to better understand its pathogenicity, the authors studied the morphological changes of nonfimbriated smooth strains induced by growth environmental humidity. tr ... | 2013 | 23573892 |
| magnolol ameliorates ligature-induced periodontitis in rats and osteoclastogenesis: in vivo and in vitro study. | periodontal disease characterized by alveolar bone resorption and bacterial pathogen-evoked inflammatory response has been believed to have an important impact on human oral health. the aim of this study was to evaluate whether magnolol, a main constituent of magnolia officinalis, could inhibit the pathological features in ligature-induced periodontitis in rats and osteoclastogenesis. the sterile, 3-0 (diameter; 0.2 mm) black braided silk thread, was placed around the cervix of the upper second ... | 2013 | 23573141 |
| detection of oral bacterial dna in synovial fluid. | as periodontal bacteria might be involved in the aetiology of rheumatic diseases, we analysed synovial fluid obtained from patients with rheumatoid arthritis (ra) and controls for the presence of dna of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, and treponema denticola. | 2013 | 23534379 |
| cluster of bacteria associated with peri-implantitis. | information on the microbiota in peri-implantitis is limited. we hypothesized that neither gender nor a history of periodontitis/smoking or the microbiota at implants differ by implant status. | 2014 | 23527870 |
| subgingival aggregatibacter actinomycetemcomitans associates with the risk of coronary artery disease. | we investigated the association between angiographically verified coronary artery disease (cad) and subgingival aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia and treponema denticola. | 2013 | 23521427 |
| ygiw and qsebc are co-expressed in aggregatibacter actinomycetemcomitans and regulate biofilm growth. | the quorum-sensing escherichia coli regulators b and c (qsebc) two-component system were previously shown to regulate biofilm growth of the oral pathogen aggregatibacter actinomycetemcomitans and to be essential for virulence. in this study, we use rt-pcr to show that an open reading frame, ygiw, residing upstream of qsebc and encoding a hypothetical protein is co-expressed with qsebc. in addition, using a series of lacz transcriptional fusion constructs and 5'-rapid amplification of cdna ends ( ... | 2013 | 23519160 |
| peptides: β-cyclodextrin inclusion compounds as highly effective antimicrobial and anti-epithelial proliferation agents. | the use of antimicrobial peptides (amps) as therapeutic agents for periodontal infections has great advantages, such as broad spectrum of action, low toxicity, and limited bacterial resistance. however, their practical use is limited because of the large amount of peptide required to exercise the microbicidal function. | 2013 | 23510146 |
| subgingival bacterial burden in relation to clinical and radiographic periodontal parameters. | this cross-sectional study characterizes the association between subgingival bacterial profile and periodontal parameters in patients assigned to coronary angiography because of cardiologic problems, which may affect the oral microbiota. | 2013 | 23489235 |
| mlc is a transcriptional activator with a key role in integrating cyclic amp receptor protein and integration host factor regulation of leukotoxin rna synthesis in aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans, a periodontal pathogen, synthesizes leukotoxin (ltxa), a protein that helps the bacterium evade the host immune response. transcription of the ltxa operon is induced during anaerobic growth. the cyclic amp (camp) receptor protein (crp) indirectly increases ltxa expression, but the intermediary regulator is unknown. integration host factor (ihf) binds to and represses the leukotoxin promoter, but neither crp nor ihf is responsible for the anaerobic induction ... | 2013 | 23475968 |
| genetic analysis of porphyromonas gingivalis (fima), aggregatibacter actinomycetemcomitans, and red complex in coronary plaque. | the objective of the present study was to detect the presence of porphyromonas gingivalis (fima), aggregatibacter actinomycetemcomitans, and red complex in the coronary plaque of patients with coronary artery disease. | 2014 | 23447375 |
| non-surgical periodontal therapy decreases serum elastase levels in aggressive but not in chronic periodontitis. | assessment of the effect of non-surgical periodontal therapy (srp) on serum inflammatory parameters in patients with untreated aggressive (agp) and chronic (chp) periodontitis. | 2013 | 23432024 |
| prevalence of periodontal pathogens and metabolic control of type 1 diabetes patients. | the aim of the study was to evaluate the prevalence of periodontal pathogens aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia and treponema denticola in subgingival plaque collected at different probing depths of type 1 diabetes patients with periodontal disease in correlation to metabolic control. | 2013 | 23413629 |
| the periodontal health of lead-exposed children living in a shipyard industrial area. | in a cross-sectional design, 292 schoolchildren living around a shipyard area, known to be contaminated with lead from shipyard industry, were examined to verify the association between lead exposure and periodontal health. the probing pocket depth (pd), bleeding on probing, plaque and calculus, and the presence of aggregatibacter actinomycetemcomitans (aa) in subgingival crevices were recorded. gingival inflammation was the most common (98%) among children in the area. no significant difference ... | 2015 | 23406954 |
| the anti-phospholipid antibody-dependent and independent effects of periodontopathic bacteria on threatened preterm labor and preterm birth. | periodontal disease is considered to be a risk factor for threatened preterm labor (tpl) and preterm birth (pb), but pathogenic mechanisms have not yet been elucidated. we hypothesized that infection with periodontopathic bacteria may enhance thrombosis through molecular mimicry with tlrvyk peptides on beta-2 glycoprotein i, a target molecule in anti-phospholipid syndrome. this study aimed to examine the effects of periodontitis on tpl and pb. | 2013 | 23400354 |
| phenotypic and genotypic features of aggregatibacter actinomycetemcomitans isolated from patients with periodontal disease. | aggregatibacter actinomycetemcomitans is strongly implicated in the pathogenesis of periodontitis. in this study, the phenotypic and genotypic features of a. actinomycetemcomitans and the presence of genes involved in toxicity were determined. sixty-five patients with periodontal pocket and 48 healthy subjects were evaluated. biotyping, adherence and invasion, neuraminidase and biofilm production, presence of capsule and fimbria, as well as the presence of flp-1, apah, ltx, and cdt genes were de ... | 2013 | 23391608 |
| in vitro antimicrobial effects of two antihalitosis mouth rinses on oral pathogens and human tongue microbiota. | the aim of the study was to compare the antimicrobial activity of a mouth rinse containing chlorhexidine and cetylpyridinium chloride (mr1) with a stannous fluoride-based mouth rinse (mr2) in vitro. | 2013 | 23368885 |
| periodontal pathogen accelerates lipid peroxidation and atherosclerosis. | recent studies have shown an association between periodontal disease and cardiovascular disease. we previously reported that intravenous challenge with aggregatibacter actinomycetemcomitans (aa) accelerated atherosclerosis in apolipoprotein e-deficient spontaneously hyperlipidemic (apoe(shl)) mice. in this study, we investigated whether live cells were required for atherosclerosis induction or whether lipopolysaccharide (lps) alone was sufficient to increase atherosclerotic damage. mice were inj ... | 2013 | 23355524 |
| specific infections as the etiology of destructive periodontal disease: a systematic review. | destructive periodontal disease has been primarily defined and investigated as an infectious disease. the aim of this study was to systematically search for cohort studies where microbiological diagnoses were performed before the onset of destructive periodontal disease and where statistically significant associations were identified. a search was executed in pubmed. the results showed that three studies published after 2005 supported the infection hypothesis for one putative periodontal pathoge ... | 2013 | 23331417 |
| lactoferrin knockout mice demonstrates greater susceptibility to aggregatibacter actinomycetemcomitans-induced periodontal disease. | among the innate defense mechanisms in the oral cavity, lactoferrin (lf) is a vital antimicrobial that can modify the host response against periodontopathogens. aggregatibacter actinomycetemcomitans is the main periodontopathogen of localized aggressive periodontitis. the aim of this study is to evaluate the role of lf during a. actinomycetemcomitans-induced periodontitis. | 2013 | 23327622 |
| a. actinomycetemcomitans lps enhances foam cell formation induced by ldl. | the objective of this study was to examine whether native low-density lipoprotein (ldl) induces foam cell formation by macrophages and to examine the effect of lipopolysaccharide (lps) on native ldl-induced foam cell formation by macrophages in vitro. raw 264.7 cells were cultured with ldl or high-density lipoprotein (hdl) in the presence of lps derived from aggregatibacter actinomycetemcomitans. foam cell formation was determined by staining with oil-red-o to visualize cytoplasmic lipid droplet ... | 2013 | 23318766 |
| in vitro antimicrobial and anticancer potential of hinokitiol against oral pathogens and oral cancer cell lines. | hinokitiol is a natural component isolated from chamacyparis taiwanensis. it has anti-microbial activity, and has been used in oral care products. the minimal inhibitory concentration (mic) and minimal microbicidal concentration (mmc) of hinokitiol against mrsa, aggregatibacter actinomycetemcomitans, streptococcus mutans, and candida albicans were determined by the agar and broth dilution method (mic: 40-110μm; mmc: 50-130μm); the paradoxical inhibition phenomenon (pip) was observed in a. actino ... | 2013 | 23312825 |
| irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by aggregatibacter actinomycetemcomitans. | periodontitis is an infectious inflammatory disease. our previous studies have revealed that irsogladine maleate (im) regulates intercellular junctional function and chemokine secretion in gingival epithelium, resulting in the suppression of the onset of periodontal disease in a rat model. therefore, it is plausible that im is a promising preventive remedy for periodontal disease. in this study, to gain a better understanding of im in gingival epithelial cells, we employed a dna microarray analy ... | 2013 | 23306101 |
| evaluation of three selective media for isolation of aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans is a pathogen in oral and nonoral infections. detection and quantification of this pathogen can be performed using selective culture techniques. the aim of this study was to establish the efficacy of two known selective media in their ability to select and support the growth of a. actinomycetemcomitans. | 2013 | 23278666 |
| azithromycin kills invasive aggregatibacter actinomycetemcomitans in gingival epithelial cells. | aggregatibacter actinomycetemcomitans invades periodontal pocket epithelium and is therefore difficult to eliminate by periodontal scaling and root planing. it is susceptible to azithromycin, which is taken up by many types of mammalian cells. this led us to hypothesize that azithromycin accumulation by gingival epithelium could enhance the killing of intraepithelial a. actinomycetemcomitans. [(3)h]azithromycin transport by smulow-glickman gingival epithelial cells and scc-25 oral epithelial cel ... | 2013 | 23274657 |
| evaluating the antimicrobial activity of commercially available herbal toothpastes on microorganisms associated with diabetes mellitus. | the present study was conducted to evaluate the effcacy of commercially available herbal toothpastes against the different periodontopathogens. | 2013 | 24685799 |
| essential role of jak/stat pathway in the induction of cell cycle arrest in macrophages infected with periodontopathic bacterium aggregatibacter actinomycetemcomitans. | in the present study, phosphorylation of signal transducers and activators of transcription 3 (stat3) was found to be important in the induction of g1 cell cycle arrest in murine macrophages infected with aggregatibacter actinomycetemcomitans. first, we focused on suppressor of cytokine signaling 3 (socs3) as a negative regulator of the jak/stat pathway. flow cytometric analysis showed that a. actinomycetemcomitans infection eliminated g1 cell cycle arrest in socs3-overexpressing raw 264.7 macro ... | 2013 | 23266654 |
| monodisperse and lps-free aggregatibacter actinomycetemcomitans leukotoxin: interactions with human β2 integrins and erythrocytes. | aggregatibacter actinomycetemcomitans is a gram-negative, facultatively anaerobic cocco-bacillus and a frequent member of the human oral flora. it produces a leukotoxin, ltxa, belonging to the repeats-in-toxin (rtx) family of bacterial cytotoxins. ltxa efficiently kills neutrophils and mononuclear phagocytes. the known receptor for ltxa on leukocytes is integrin α(l)β(2) (lfa-1 or cd11a/cd18). however, the molecular mechanisms involved in ltxa-mediated cytotoxicity are poorly understood, partly ... | 2013 | 23234758 |
| can salivary activity predict periodontal breakdown in a. actinomycetemcomitans infected adolescents? | while aggregatibacter actinomycetemcomitans (aa) is highly associated with localised aggressive periodontitis (lap) many aa-carriers do not develop lap. this study was designed to determine whether specific salivary factors could distinguish between subjects who have aa initially and remain healthy (h/aa) as compared to those who develop lap (lap/aa). | 2013 | 23219180 |
| association of serum anti-periodontal pathogen antibody with ischemic stroke. | periodontitis increases the risk of atherosclerotic cardiovascular disease and ischemic stroke. in this study, we evaluated whether serum antibody levels against individual periodontal pathogens are significantly associated with ischemic stroke subtypes and their risk factors. | 2012 | 23207319 |
| comparative genomic hybridization and transcriptome analysis with a pan-genome microarray reveal distinctions between jp2 and non-jp2 genotypes of aggregatibacter actinomycetemcomitans. | it was postulated that the highly virulent jp2 genotype of aggregatibacter actinomycetemcomitans may possess a constellation of distinct virulence determinants not found in non-jp2 genotypes. this study compared the genome content and the transcriptome of the serotype b jp2 genotype and the closely related serotype b non-jp2 genotype of a. actinomycetemcomitans. a custom-designed pan-genomic microarray of a. actinomycetemcomitans was constructed and validated against a panel of 11 sequenced refe ... | 2013 | 23194436 |
| alpha-amylase is a human salivary protein with affinity to lipopolysaccharide of aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans lipopolysaccharide (aa.lps) is a major virulence factor associated with aggressive periodontitis. although the recognition of aa.lps is potentially initiated by salivary proteins in the oral cavity, aa.lps-binding proteins (aa.lps-bps) in saliva are poorly characterized. the purpose of this study was to capture and identify aa.lps-bps in human saliva using a ltq-orbitrap hybrid fourier transform mass spectrometry. aa.lps conjugated onto n-hydroxysuccinimidyl ... | 2013 | 23194029 |
| influence of periodontal status and periodontopathogens on levels of oral human β-defensin-2 in saliva. | expression patterns of human β-defensin-2 (hbd-2) mrna or hbd-2 protein concentration and periodontal diseases have been a focus of scientific research. this study compares the salivary levels of hbd-2 protein concentration of healthy patients and patients with gingivitis and chronic periodontitis (cp) and correlates these levels with the presence of periodontopathogens. | 2013 | 23173827 |
| protective effect of topical cordia verbenacea in a rat periodontitis model: immune-inflammatory, antibacterial and morphometric assays. | this study evaluated the effects of c. verbenacea essential oil topically administered in a rat periodontitis model. | 2012 | 23171319 |
| p2x receptor stimulation amplifies complement-induced haemolysis. | activation of the complement system evokes cell damage by insertion of membrane attack complexes, which constitute the basis of the pathogenesis of various haemolytic disorders. recently, we found that haemolysis caused by other types of membrane pore-forming proteins such as α-haemolysin (hlya) from escherichia coli, α-toxin from staphylococcus aureus and leukotoxin from aggregatibacter actinomycetemcomitans inflict their cytotoxic effects through p2 receptor activation. here we show that simil ... | 2013 | 23149487 |
| screening for periodontopathogenic bacteria in severe chronic periodontitis in a moroccan population. | bacteria play an important role in destructive periodontitis. the aim of this study was to screen for five highly pathogenic bacteria: aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, and treponema denticola, in moroccan patients presenting with severe chronic periodontitis and to compare results with those of patients presenting with severe aggressive periodontitis. | 2012 | 23141872 |
| gram-positive bacteria as an antigen topically applied into gingival sulcus of immunized rat accelerates periodontal destruction. | periodontitis is generally accepted to relate to gram-negative bacteria, and the host defense system influences its onset and progression. however, little is known about the relation between gram-positive bacteria and periodontitis. in this study, we topically applied gram-positive and gram-negative bacterial suspensions to the gingival sulcus in rats after immunization, and then histopathologically examined their influence on periodontal destruction. | 2013 | 23137272 |
| frequency of periodontal pathogens in equivalent peri-implant and periodontal clinical statuses. | this study tested the hypotheses that there is: (1) higher bacterial frequency in peri-implantitis/periodontitis, followed by mucositis/gingivitis and peri-implant/periodontal health; (2) similar bacterial frequency between comparable peri-implant and periodontal clinical statuses. | 2013 | 23127822 |
| possible translocation of periodontal pathogens into the lymph nodes draining the oral cavity. | numerous publications have reported the presence of periodontopathogenic bacteria in peripheral and central vascular lesions. however, it is unclear how this bacterial translocation occurs. the objective of this study was to investigate whether periodontopathic bacteria are translocated to lymph nodes proximal to the oral cavity. obtaining lymph node samples is not ethically feasible unless they are excised as part of the surgical management of patients with cancer. this study analyzed formalin- ... | 2012 | 23124752 |
| transcriptional regulation of aggregatibacter actinomycetemcomitans lsracdbfg and lsrrk operons and their role in biofilm formation. | autoinducer-2 (ai-2) is required for biofilm formation and virulence of the oral pathogen aggregatibacter actinomycetemcomitans, and we previously showed that lsrb codes for a receptor for ai-2. the lsrb gene is expressed as part of the lsracdbfg operon, which is divergently transcribed from an adjacent lsrrk operon. in escherichia coli, lsrrk encodes a repressor and ai-2 kinase that function to regulate lsracdbfg. to determine if lsrrk controls lsracdbfg expression and influences biofilm growth ... | 2013 | 23104800 |
| modeling susceptibility to periodontitis. | chronic inflammatory diseases like periodontitis have a complex pathogenesis and a multifactorial etiology, involving complex interactions between multiple genetic loci and infectious agents. we aimed to investigate the influence of genetic polymorphisms and bacteria on chronic periodontitis risk. we determined the prevalence of 12 single-nucleotide polymorphisms (snps) in immune response candidate genes and 7 bacterial species of potential relevance to periodontitis etiology, in chronic periodo ... | 2013 | 23100272 |
| effects of biphenyl sulfonylamino methyl bisphosphonic acids on porphyromonas gingivalis and cytokine secretion by oral epithelial cells. | bisphosphonate drugs are well known to inhibit osteoclastic activity and have been proposed for the management of bone diseases, including periodontitis which is associated with alveolar bone destruction. in this study, we evaluated the effects of four arylsulfonamide bisphosphonates on growth of the periodontopathogenic bacterium porphyromonas gingivalis as well as their capacity to reduce cytokine secretion by lipopolysaccharide (lps)-stimulated oral epithelial cells. the growth of p. gingival ... | 2013 | 23061603 |
| clinical and microbiological changes after minimally invasive therapeutic approaches in intrabony defects: a 12-month follow-up. | this 12-month randomized, controlled trial evaluated the clinical effects and microbiological changes of minimally invasive nonsurgical and surgical approaches for the therapy of intrabony defects. | 2013 | 23053707 |
| reduction of salivary arginine catabolic activity through periodontal therapy. | salivary enzymes may be used to diagnose periodontal conditions. salivary arginase activity (saa) is related to susceptibility to bacterial infection. therefore, the aim of this controlled interventional study was to determine the saa before and after non-surgical periodontal therapy. | 2012 | 23041992 |
| periodontal pathogen aggregatibacter actinomycetemcomitans deteriorates pressure overload-induced myocardial hypertrophy in mice. | although a relationship between periodontitis and myocardial hypertrophy has been reported, the precise mechanism has not been clarified. the purpose of this study was to investigate the association between periodontal infection and myocardial hypertrophy. transverse aortic constriction (tac) was performed. mice were injected with aggregatibacter actinomycetemcomitans (a.a.) (0.1 ml of 10(8) cfu/ml) in the infected group and pbs in the control group. echocardiography, histopathology, and immunoh ... | 2012 | 23038095 |
| variables of host response in saliva of patients with periodontitis: a pilot study. | periodontitis is an infection that results from an imbalance between periodontopathic microorganisms and the local and systemic host defense. this study analyzed saliva samples of patients with periodontitis for several biomarkers of host response. | 2012 | 23034426 |
| porphyromonas gingivalis displays a competitive advantage over aggregatibacter actinomycetemcomitans in co-cultured biofilm. | biofilm formation occurs through the events of cooperative growth and competitive survival among multiple species. porphyromonas gingivalis and aggregatibacter actinomycetemcomitans are important periodontal pathogens. the aim of this study was to demonstrate competitive or cooperative interactions between these two species in co-cultured biofilm. | 2013 | 23033940 |
| an evolutionary link between natural transformation and crispr adaptive immunity. | natural transformation by competent bacteria is a primary means of horizontal gene transfer; however, evidence that competence drives bacterial diversity and evolution has remained elusive. to test this theory, we used a retrospective comparative genomic approach to analyze the evolutionary history of aggregatibacter actinomycetemcomitans, a bacterial species with both competent and noncompetent sister strains. through comparative genomic analyses, we reveal that competence is evolutionarily lin ... | 2012 | 23033473 |
| prophage induction in lysogenic aggregatibacter actinomycetemcomitans cells co-cultured with human gingival fibroblasts, and its effect on leukotoxin release. | lysogeny is common among strains of the periodontal pathogen aggregatibacter actinomycetemcomitans. since lysogenic induction is known to result in the increased synthesis and release of bacterial toxins from lysogens, it would be important to elucidate the conditions under which induction of these bacteria may occur. co-cultures of a. actinomycetemcomitans strains (either lysogenic or non-lysogenic) and human cells (either gingival fibroblasts or pharyngeal epithelial cells) were prepared. foll ... | 2013 | 23022667 |
| aggregatibacter actinomycetemcomitans in down's syndrome children. | to investigate the presence of aggregatibacter actinomycetemcomitans (aa) in down's syndrome (ds) children in comparison with age-matched normal children and to determine if aa was acquired during childhood in ds individuals. | 2012 | 23019843 |
| detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women. | the aim of this study was to use the fluorescence in situ hybridization (fish) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. this cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque samples were collected and the fish ... | 2012 | 23018230 |
| molecular detection of aggregatibacter actinomycetemcomitans on metallic brackets by the checkerboard dna-dna hybridization technique. | the purpose of this randomized clinical study was to evaluate the presence of the periodontal pathogen aggregatibacter actinomycetemcomitans on metallic brackets and the effectiveness of a 0.12% chlorhexidine digluconate mouthwash in inhibiting this microorganism. | 2012 | 22999671 |
| a double-blind randomized placebo-controlled study on the clinical and microbial effects of an essential oil mouth rinse used by patients in supportive periodontal care. | this 3-month double-blind randomized placebo-controlled study evaluated the clinical and microbial effects of an essential oil mouth rinse used as an adjunct to mechanical plaque control by patients in supportive periodontal care. | 2013 | 22998386 |
| quantification of periodontopathic bacteria in saliva using the invader assay. | when quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. the invader assay is a convenient method because it does not involve gene amplification. the purpose of this study was to evaluate the validity of the invader assay to quantify periodontopathic bacteria. the invader technology was applied in quantifying five periodontopathic bacteria (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsy ... | 2012 | 22996215 |
| do elderly edentulous patients with a history of periodontitis harbor periodontal pathogens? | the presence of periodontal pathogens in the oral cavity may impact implant survival. therefore, this study aimed to determine the prevalence of campylobacter rectus, porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, prevotella intermedia, tannerella forsythia, treponema denticola, eikenella corrodens, and parvimonas micra in a specific elderly population with a history of periodontitis who have never worn dentures. | 2012 | 22988660 |
| sustained mitogen-activated protein kinase activation with aggregatibacter actinomycetemcomitans causes inflammatory bone loss. | aggregatibacter actinomycetemcomitans is a gram-negative facultative capnophile involved in pathogenesis of aggressive forms of periodontal disease. in the present study, we interrogated the ability of a. actinomycetemcomitans to stimulate innate immune signaling and cytokine production and established that a. actinomycetemcomitans causes bone loss in a novel rat calvarial model. in vitro studies indicated that a. actinomycetemcomitans stimulated considerable production of soluble cytokines, tum ... | 2012 | 22958388 |
| characterization of the secretion pathway of the collagen adhesin emaa of aggregatibacter actinomycetemcomitans. | the extracellular matrix protein adhesin a (emaa) surface antennae-like structures of the periodontal pathogen aggregatibacter actinomycetemcomitans are composed of three identical protein monomers. recently, we have demonstrated that the protein is synthesized with an extended signal peptide of 56 amino acids necessary for membrane targeting and protein translocation. in this study, emaa secretion was demonstrated to be reliant on a chaperone-dependent secretion pathway. deletion of secb partia ... | 2012 | 22958387 |
| analysis of viable vs. dead aggregatibacter actinomycetemcomitans and porphyromonas gingivalis using selective quantitative real-time pcr with propidium monoazide. | one of the major disadvantages of dna-based microbial diagnostics is their inability to differentiate dna between viable and dead microorganisms, which could be important when studying etiologically relevant pathogens. the aim of this investigation was to optimize a method for the selective detection and quantification of only viable aggregatibacter actinomycetemcomitans and porphyromonas gingivalis cells by combining quantitative real-time polymerase chain reaction (qpcr) and propidium monoazid ... | 2013 | 22957816 |
| influence of il-6 haplotypes on clinical and inflammatory response in aggressive periodontitis. | the aim of this study was to investigate the inflammatory response in aggressive periodontitis (agp) patients after periodontal therapy and associate these changes to subjects' interleukin-6 (il-6) genetic variants. | 2013 | 22918663 |
| leukotoxin from aggregatibacter actinomycetemcomitans causes shrinkage and p2x receptor-dependent lysis of human erythrocytes. | leukotoxin (ltxa) is a virulence factor secreted by the bacterium aggregatibacter actinomycetemcomitans, which can cause localized aggressive periodontitis and endocarditis. ltxa belongs to the repeat-in-toxin (rtx) family of exotoxins of which other members inflict lysis by formation of membrane pores. recently, we documented that the haemolytic process induced by another rtx toxin [α-haemolysin (hlya) from escherichia coli] requires p2x receptor activation and consists of sequential cell shrin ... | 2012 | 22906303 |
| the peri-implant health in patients attending an annual recall program. a clinical and microbiological study in 74 patients from the tübingen implant registry. | to describe the peri-implant condition of unselected implant recall patients, to relate it to relevant features from the patient medical history, and to look at associations across these findings. | 2013 | 22905716 |
| interleukin-1β is internalised by viable aggregatibacter actinomycetemcomitans biofilm and locates to the outer edges of nucleoids. | the opportunistic pathogen aggregatibacter actinomycetemcomitans causes periodontitis, which is a biofilm infection that destroys tooth-supportive tissues. interleukin (il)-1β, a central proinflammatory cytokine of periodontitis, is an essential first line cytokine for local inflammation that modulates the cell proliferation and anti-pathogen response of human gingival keratinocytes. previously, we demonstrated that a. actinomycetemcomitans biofilms bind il-1β; however, whether this binding is a ... | 2012 | 22898394 |
| bacterial antagonism against periodontopathogens. | the aim of the current study is to compare the prevalence of commensal bacteria, with beneficial properties, for healthy and diseased individuals and additionally to examine the inhibitory effect of some commercial dietary probiotics on periodontopathogens, comparing this inhibitory effect to that of orally derived beneficial bacteria. | 2013 | 22897652 |
| antibiotic susceptibility profiles of oral pathogens. | periodontitis is a bacterial disease that can be treated with systemic antibiotics. the aim of this study was to establish the antibiotic susceptibility profiles of five periodontal pathogens to six commonly used antibiotics in periodontics. a total of 247 periodontal bacterial isolates were tested for susceptibility to the six antibiotics using the etest method. mic(50) and mic(90) values (minimum inhibitory concentrations for 50% and 90% of the organisms, respectively) were calculated. both eu ... | 2012 | 22890195 |
| periodontal pathogen load and increased antibody response to heat shock protein 60 in patients with cardiovascular disease. | to determine the relationship between periodontal pathogen load and anti-human heat shock protein 60 (hhsp60) antibodies in patients with established cardiovascular disease (cvd). | 2012 | 22882677 |
| the periodontal pathogen aggregatibacter actinomycetemcomitans deteriorates ventricular remodeling after myocardial infarction in mice. | chronic inflammation plays a fundamental role in coronary heart disease (chd). periodontal disease is a common infectious disease and is a potential source of systemic inflammation. however, the effect of periodontal infection on chd has not yet been proven. the purpose of this study was to determine the effect of periodontopathic bacteria on experimental myocardial infarction (mi). we implanted a chamber into the subcutaneous tissue of each male mouse. aggregatibacter actinomycetemcomitans (a.a ... | 2012 | 22878805 |
| prediagnostic plasma antibody levels to periodontopathic bacteria and risk of coronary heart disease. | many epidemiological studies have indicated that periodontitis is an important risk factor for coronary heart disease (chd). we examined whether plasma antibody levels to 3 major periodontal pathogens, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia predicted the risk of chd events. a nested case-control research design (case: n = 191, control: n = 382), by matching gender, age, study area, date of blood collection, and time since last meal at blood col ... | 2012 | 22878796 |
| are there specific benefits of amoxicillin plus metronidazole in aggregatibacter actinomycetemcomitans-associated periodontitis? double-masked, randomized clinical trial of efficacy and safety. | it has been suggested that prescription of amoxicillin plus metronidazole in the context of periodontal therapy should be limited to patients with specific microbiologic profiles, especially those testing positive for aggregatibacter actinomycetemcomitans. the main purpose of this analysis is to determine if patients positive for a. actinomycetemcomitans with moderate to advanced periodontitis benefit specifically from amoxicillin plus metronidazole given as an adjunct to full-mouth scaling and ... | 2013 | 22873656 |
| antimicrobial peptides and nitric oxide production by neutrophils from periodontitis subjects. | neutrophils play an important role in periodontitis by producing nitric oxide (no) and antimicrobial peptides, molecules with microbicidal activity via oxygen-dependent and -independent mechanisms, respectively. it is unknown whether variation in the production of antimicrobial peptides such as ll-37, human neutrophil peptides (hnp) 1-3, and no by neutrophils influences the pathogenesis of periodontal diseases. we compared the production of these peptides and no by lipopolysaccharide (lps)-stimu ... | 2012 | 22850872 |
| [fungal invasion of connective tissue in patients with gingival-periodontal disease]. | in the last few years unusual microorganisms have been isolated from subgingival biofilm, as possible initiators or contributors to periodontal disease, especially in patients who show no improvement during treatment. | 2015 | 22824245 |
| an in vitro investigation concerning the bacterial leakage at implants with internal hexagon and morse taper implant-abutment connections. | to evaluate, in vitro, the leakage observed in internal hexagon and morse taper implant-abutment connections. | 2012 | 22814560 |
| serotype-dependent expression patterns of stabilized lipopolysaccharide aggregates in aggregatibacter actinomycetemcomitans strains. | above a critical concentration, amphiphilic lipopolysaccharide (lps) molecules in an aqueous environment form aggregate structures, probably because of interactions involving hydrophobic bonds. ionic bonds involving divalent cations stabilize these aggregate structures, making them resistant to breakdown by detergents. the aim of this study was to examine expression patterns of stabilized lps aggregates in aggregatibacter actinomycetemcomitans, a microorganism that causes periodontitis. a. actin ... | 2012 | 22812537 |
| clinical and microbiological parameters in patients with self-ligating and conventional brackets during early phase of orthodontic treatment. | to determine the effect of different bracket designs (conventional brackets and self-ligating brackets) on periodontal clinical parameters and periodontal pathogens in subgingival plaque. | 2013 | 22765511 |
| antimicrobial activity of nanoparticulate metal oxides against peri-implantitis pathogens. | dental plaque accumulation may result in peri-implantitis, an inflammatory process causing loss of supporting bone that may lead to dental implant failure. the antimicrobial activities of six metal and metal oxide nanoparticles and two of their composites against bacterial pathogens associated with peri-implantitis were examined under anaerobic conditions. the activities of nanoparticles of silver (ag), cuprous oxide (cu(2)o), cupric oxide (cuo), zinc oxide (zno), titanium dioxide (tio(2)), tung ... | 2012 | 22727529 |
| [amalgam, composite and compomer: microbiological study]. | restorative materials have different consequences on the periodontium. the surface of these materials may influence gingival health and cause in some instances gingival inflammation. the purpose of this study was to investigate and compare, in a healthy periodontium, intracrevicular plaque bacteria (aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis and treponema denticola), at day 0 and at 6 months, around subgingivally located amalga ... | 2012 | 22715643 |
| subgingival microbiota from cebus apella (capuchin monkey) with different periodontal conditions. | this present study evaluated the subgingival microbiota of the cebus apella with different periodontal conditions kept by the tufted capuchin monkey procreation center (são paulo state university - unesp) or free-ranging monkeys. for this purpose, clinical specimens of subgingival biofilm were collected from 52 monkeys, of both genders, 40 kept in captivity and 12 free-ranging monkeys. the primates were submitted to periodontal evaluation and biofilm samples were transferred to vmga iii transpor ... | 2012 | 22710412 |
| exogenous administration of 15d-pgj2-loaded nanocapsules inhibits bone resorption in a mouse periodontitis model. | the 15-deoxy-(δ12,14)-pg j(2) (15d-pgj(2)) has demonstrated excellent anti-inflammatory results in different experimental models. it can be used with a polymeric nanostructure system for modified drug release, which can change the therapeutic properties of the active principle, leading to increased stability and slower/prolonged release. the aim of the current study was to test a nanotechnological formulation as a carrier for 15d-pgj(2), and to investigate the immunomodulatory effects of this fo ... | 2012 | 22706081 |
| a common periodontal pathogen has an adverse association with both acute and stable coronary artery disease. | the aim of this study was to investigate the association between angiographically verified coronary artery disease (cad) and salivary levels of four major periodontal pathogens. | 2012 | 22704805 |
| o-polysaccharide glycosylation is required for stability and function of the collagen adhesin emaa of aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans is hypothesized to colonize through the interaction with collagen and establish a reservoir for further dissemination. the trimeric adhesin emaa of a. actinomycetemcomitans binds to collagen and is modified with sugars mediated by an o-antigen polysaccharide ligase (waal) that is associated with lipopolysaccharide (lps) biosynthesis (g. tang and k. mintz, j. bacteriol. 192:1395-1404, 2010). this investigation characterized the function and cellular localizat ... | 2012 | 22689812 |
| peripheral arterial disease associated with caries and periodontal disease. | peripheral arterial disease (pad) is an important cardiovascular disorder of the peripheral arteries. chronic infections, such as periodontitis, may play an important role in the etiology and pathophysiology of pad and other cardiovascular conditions. recently, streptococcus mutans has been found with high frequency in atheromatous plaques. the aim of this study is to evaluate the possible clinical and microbiologic association between pad and periodontitis and dental caries. | 2013 | 22680302 |
| relationship between gram negative enteric rods, aggregatibacter actinomycetemcomitans, and clinical parameters in periodontal disease. | the association between gram negative enteric rods and aggregatibacter actinomycetemcomitans in periodontal diseases has received little attention in the literature. the objective of this study was to explore the relationship between these organisms and clinical parameters of periodontal disease. | 2012 | 22628966 |
| fcγriib-nt645+25a/g gene polymorphism and periodontitis in japanese women with preeclampsia. | fcγriib contains a unique immunoreceptor tyrosine-based inhibition motif (itim) and functions as a negative feedback regulator of leucocyte activation and antibody production. we have previously reported fcγriib-nt645+25a/g gene polymorphism to be associated with prevalence and severity of periodontitis, fcγriib expression level on peripheral b lymphocytes and the serum igg level against periodontopathic bacteria. previous studies have reported maternal periodontal disease to be associated with ... | 2012 | 22594540 |
| human salivary cystatin sa exhibits antimicrobial effect against aggregatibacter actinomycetemcomitans. | healthy subjects who do not have aggregatibacter actinomycetemcomitans in their oral cavity may possess factors in saliva that might demonstrate antibacterial activity against the bacterium. the aim of this study was to identify and purify proteins from saliva of healthy subjects that might demonstrate antibacterial activity against a. actinomycetemcomitans and test the same against the bacteria. | 2012 | 22582873 |
| experimental arthritis exacerbates aggregatibacter actinomycetemcomitans-induced periodontitis in mice. | this study aimed to investigate whether chronic antigen-induced arthritis (aia) influences infection-induced periodontitis (pd) in mice and whether pd modifies the clinical course of aia. the contribution of anti-tnf-α therapy was also evaluated. | 2012 | 22582749 |
| effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. | emingil g, han b, özdemir g, tervahartiala t, vural c, atilla g, baylas h, sorsa t. the effect of azithromycin, as an adjunct to nonsurgical periodontal treatment, on microbiological parameters and gingival crevicular fluid biomarkers in generalized aggressive periodontitis. j periodont res 2012; 47: 729-739. © 2012 john wiley & sons a/s background and objective: to study the effectiveness of azithromycin in combination with nonsurgical periodontal therapy on clinical and microbiological parame ... | 2012 | 22571226 |
| subgingival microbial profiles as diagnostic markers of destructive periodontal diseases: a clinical epidemiology study. | to describe the subgingival microbial profiles of the major putative periodontal pathogens and investigate their role as diagnostic markers for destructive periodontal diseases in an untreated and isolated population. | 2013 | 22564019 |
| interactions of extracts from selected chewing stick sources with aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans produces a leukotoxin that activates a pro-inflammatory death of human monocytes/macrophages. a specific clone of this bacterium (jp2) has a 530-base pair deletion in the leukotoxin promoter gene and significantly enhanced expression of leukotoxin. this specific clone of a. actinomycetemcomitans is common in some african populations and has a strong association with periodontal attachment loss in adolescents in these populations. chewing sticks of plant orig ... | 2012 | 22537711 |
| bacterial and inflammatory behavior of implants in the early healing phase of chronic periodontitis. | to assess the pattern of early bacterial colonization at implants and teeth in patients with a history of chronic periodontitis compared with a group of healthy subjects. furthermore, the presence of host-derived markers at teeth and implants in the two subject groups was determined. | 2012 | 22532956 |
| quantification of subgingival bacterial pathogens at different stages of periodontal diseases. | anaerobic gram-negative oral bacteria such as treponema denticola, aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, tannerella forsythia, campylobacter rectus, and fusobacterium nucleatum are closely associated with periodontal diseases. we measured the relative population (bacterial levels) of these oral pathogens in subgingival tissues of patients at different stages of korean chronic periodontal diseases. we divided the individuals into those with chronic gingivitis (g), moder ... | 2012 | 22526568 |
| mast cells act as phagocytes against the periodontopathogen aggregatibacter actinomycetemcomitans. | evidence to date shows that mast cells play a critical role in immune defenses against infectious agents, but there have been no reports about involvement of these cells in eliminating periodontopathogens. in this study, the phagocytic ability of mast cells against aggregatibacter actinomycetemcomitans compared with macrophages is evaluated. | 2013 | 22524328 |
| involvement of toll-like receptor 2 in apoptosis of aggregatibacter actinomycetemcomitans-infected thp-1 cells. | aggregatibacter (actinobacillus) actinomycetemcomitans is a gram-negative bacterium that has been associated with aggressive periodontitis. a actinomycetemcomitans infection induces apoptosis in the human monocytic cell line thp-1, and p38 mitogen-activated protein kinase (p38) activity and tumor necrosis factor-α (tnf-α) production are enhanced by a actinomycetemcomitans infection. however, mechanisms governing the recognition of a actinomycetemcomitans by monocytes during apoptosis have not be ... | 2013 | 22513358 |
| bacterial colonization patterns of periodontal pockets in different ages. | the aim of this study was to investigate subgingival bacterial composition of untreated italian subjects with aggressive and chronic periodontitis. the total bacterial load, pathogenic bacteria belonging to "red" and "orange" complexes and aggregatibacter actinomycetemcomitans were determined by real-time pcr in 1216 patients. data were analysed by looking for relationships between bacteriological parameters, age and periodontal probing depth. the obtained results showed a significant higher num ... | 2013 | 22507992 |
| aggregatibacter actinomycetemcomitans targets nlrp3 and nlrp6 inflammasome expression in human mononuclear leukocytes. | periodontitis is an inflammatory condition that destroys the tooth-supporting tissues, as a result of local bacterial infection. aggregatibacter actinomycetemcomitans is a gram-negative facultative anaerobic species, highly associated with aggressive periodontitis. periodontal inflammation is dominated by cytokines of the interleukin (il)-1 family. prior to their secretion by mononuclear cells, il-1 cytokines are processed by intracellular protein complexes, known as "inflammasomes", which can s ... | 2012 | 22503597 |
| production of a novel quercetin glycoside through metabolic engineering of escherichia coli. | most flavonoids exist as sugar conjugates. naturally occurring flavonoid sugar conjugates include glucose, galactose, glucuronide, rhamnose, xylose, and arabinose. these flavonoid glycosides have diverse physiological activities, depending on the type of sugar attached. to synthesize an unnatural flavonoid glycoside, actinobacillus actinomycetemcomitans gene tll (encoding dtdp-6-deoxy-l-lyxo-4-hexulose reductase, which converts the endogenous nucleotide sugar dtdp-4-dehydro-6-deoxy-l-mannose to ... | 2012 | 22492444 |
| aggregatibacter actinomycetemcomitans lipopolysaccharide regulates bone sialoprotein gene transcription. | aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) is believed to be associated with aggressive periodontitis characterized by a rapid bone loss. a. actinomycetemcomitans lipopolysaccharide (lps) has a similar structure to escherichia coli lps, and they are toll-like receptor 4 agonists. bone sialoprotein (bsp) is an early marker of osteoblast differentiation. to investigate the effects of a. actinomycetemcomitans lps on bone formation, we targeted bsp as a marker for osteogenic di ... | 2012 | 22492284 |
| aggregatibacter actinomycetemcomitans serotypes, the jp2 clone and cytolethal distending toxin genes in a thai population. | to examine the genetic diversity of aggregatibacter actinomycetemcomitans in thai adults. | 2012 | 22471788 |