| multilocus sequence type profiles of bacillus cereus isolates from infant formula in china. | bacillus cereus sensu stricto is an opportunistic foodborne pathogen. the multilocus sequence type (mlst) of 74 b. cereus isolated from 513 non-random infant formula in china was analyzed. of 64 sequence types (sts) detected, 50 sts and 6 alleles were newly found in pubmlst database. all isolates except for one singleton (st-1049), were classified into 7 clonal complexes (cc) by burst (n-4), in which cc1 with core ancestral clone st-26 was the largest group including 86% isolates, and cc2, 3, 9, ... | 2017 | 27889164 |
| microbial decontamination of onion powder using microwave-powered cold plasma treatments. | the effects of microwave-integrated cold plasma (cp) treatments against spores of bacillus cereus and aspergillus brasiliensis and escherichia coli o157:h7 on onion powder were investigated. the growth of b. cereus, a. brasiliensis, and e. coli o157:h7 in the treated onion powder was assessed during storage at 4 and 25 °c, along with the physicochemical and sensory properties of the powder. onion powder inoculated with b. cereus was treated with cp using helium as a plasma-forming gas, with simu ... | 2017 | 27889137 |
| analysis of germination capacity and germinant receptor (sub)clusters of genome-sequenced bacillus cereus environmental isolates and model strains. | spore germination of 17 bacillus cereus food isolates and reference strains was evaluated using flow cytometry analysis in combination with fluorescent staining at a single-spore level. this approach allowed for rapid collection of germination data under more than 20 conditions, including heat activation of spores, germination in complex media (brain heart infusion [bhi] and tryptone soy broth [tsb]), and exposure to saturating concentrations of single amino acids and the combination of alanine ... | 2017 | 27881417 |
| characterization and control of symbiotic bacillus cereus isolated from the mid gut of anopheles subpictus grassi. | an investigation was done to find out the role of gut bacterium on the larval development and survival of anopheles subpictus, the vector responsible for the transmission of malaria. an. subpictus mosquitoes breed exclusively in stagnant water, including shrimp/fish ponds with high nutrient level. one bacterial strain (cx2) was isolated from the midgut of late third instar of mosquito larvae. the bacterial isolate was sensitive to recommended doses of tetracyclin (30 µg/disc), doxycycline (30 µg ... | 2016 | 27876960 |
| disruptions of the genes involved in lysine biosynthesis, iron acquisition, and secondary metabolisms affect virulence and fitness in metarhizium robertsii. | based on genomic analysis, polyketide synthase (pks) and nonribosomal peptide synthetase (nrps) pathways account for biosynthesis of the majority of the secondary metabolites produced by the entomopathogenic fungus metarhizium robertsii. to evaluate the contribution of these pathways to m. robertsii fitness and/or virulence, mutants deleted for mrppta, the sfp-type 4' phosphopantetheinyl transferase gene required for their activation were generated. δmrppta strains were deficient in pks and nrps ... | 2017 | 27876630 |
| [outbreak of foodborne disease at a mass event of indigenous peoples in cuiaba, mato grosso, brazil, in 2013]. | to report the experience of investigating the outbreak of acute diarrhoea (ad) at the xii indigenous games in cuiabá, mato grosso, brazil, 2013. | 2016 | 27861693 |
| differences in chemical, physical and microbiological characteristics of italian burrata cheeses made in artisanal and industrial plants of apulia region. | the burrata cheese is a traditional product from southern italy, consisting of an envelope of pasta filata (stretched curd) filled with cream and pasta filata strips (usually leftovers from mozzarella production). physical [water activity (aw), ph], chemical (moisture, nacl content) and microbiological [total viable count (tvc), listeria monocytogenes, salmonella spp., yersinia enterocolitica, bacillus cereus, escherichia coli, enterobacteriaceae, coagulase-positive staphylococci] characteristic ... | 2016 | 27853715 |
| shelf life evaluation of ricotta fresca sheep cheese in modified atmosphere packaging. | ricotta fresca cheese is the product of sardinian dairy industry most exposed to microbial post-process contamination. due to its technological characteristics, intrinsic parameters, ph (6.10-6.80) and water activity (0.974-0.991), it represents an excellent substrate for the growth of spoilage and pathogenic microorganisms, which are usually resident in cheese-making plants environments. generally, ricotta fresca has a shelf life of 5-7 days. for this reason, at industrial level, modified atmos ... | 2016 | 27853705 |
| characterization of three autolysins with activity against cereulide-producing bacillus isolates in food matrices. | bacillus cereus is a pathogen related with diarrhoeal or emetic food poisoning cases, of which the latter caused by the cereulide-producing isolates are more severe with several reported lethal cases. it is therefore necessary to develop an effective strategy to prevent the propagation of b. cereus in the food supply. in this study, three autolysins from the cereulide-producing b. cereus group isolates, lysis075, lysf8819.1 and lyscer057, were identified and characterized. the results showed tha ... | 2017 | 27835772 |
| bacillus cereus strain isolated from demodex folliculorum in patients with topical steroid-induced rosaceiform facial dermatitis. | the aim of the study was to identify bacillus species from the demodex folliculorum of patients with topical steroidinduced facial rosaceiform dermatitis. of the 75 patients examined, 20% had clinical spinulosis, while 18.66% had dermoscopic features of demodex: follicular plugs and tails. of the 17.33% positive patients identified upon microscopy for demodex, samples for bacterial culture were plated on trypticase soy colombia agar. identification was performed by microorganisms grown method ma ... | 2017 | 27828651 |
| use of molecular typing to investigate bacterial translocation from the intestinal tract of chlorpyrifos-exposed rats. | human are confronted on a daily basis with contaminant pesticide residues in food, water and other components of the environment. although the digestive system is the first organ to come into contact with food contaminants, very few data are available on the impact of low-dose pesticide exposure during the in utero and postnatal periods on intestinal bacterial translocation (bt). previous studies have revealed that chlorpyrifos (cpf) exposure is associated with intestinal dysbiosis and the conta ... | 2016 | 27826358 |
| optimization of the production conditions of the lipase produced by bacillus cereus from rice flour through plackett-burman design (pbd) and response surface methodology (rsm). | in this study, the screening of lipase positive bacteria from rice flour was carried out by rhodamin b agar plate method. bacillus cereus was identified by 16s rdna method. screening of the appropriate variables and optimization of the lipase production was performed using plackett-burman design (pbd) and response surface methodology (rsm). among the isolated bacteria, an aerobic bacillus cereus strain was recognized as the best lipase-producing bacteria (177.3 ± 20 u/ml). given the results, the ... | 2016 | 27816679 |
| evidence of mercury trapping in biofilm-eps and mer operon-based volatilization of inorganic mercury in a marine bacterium bacillus cereus bw-201b. | biofilm-forming mercury-resistant marine bacterium bacillus cereus bw-201b has been explored to evident that the bacterial biofilm-eps (exopolymers) trap inorganic mercury but subsequently release eps-bound mercury for induction of mer operon-mediated volatilization of inorganic mercury. the isolate was able to tolerate 50 ppm of mercury and forms biofilm in presence of mercury. mer operon-mediated volatilization was confirmed, and -sh was found to be the key functional group of bacterial eps re ... | 2017 | 27815566 |
| the large-scale process of microbial carbonate precipitation for nickel remediation from an industrial soil. | microbial carbonate precipitation is known as an efficient process for the remediation of heavy metals from contaminated soils. in the present study, a urease positive bacterial isolate, identified as bacillus cereus ns4 through 16s rdna sequencing, was utilized on a large scale to remove nickel from industrial soil contaminated by the battery industry. the soil was highly contaminated with an initial total nickel concentration of approximately 900 mg kg(-1). the soluble-exchangeable fraction wa ... | 2016 | 27814530 |
| elucidating the rimosamide-detoxin natural product families and their biosynthesis using metabolite/gene cluster correlations. | as microbial genome sequencing becomes more widespread, the capacity of microorganisms to produce an immense number of metabolites has come into better view. utilizing a metabolite/gene cluster correlation platform, the biosynthetic origins of a new family of natural products, the rimosamides, were discovered. the rimosamides were identified in streptomyces rimosus and associated with their nrps/pks-type gene cluster based upon their high frequency of co-occurrence across 179 strains of actinoba ... | 2016 | 27809474 |
| biodegradation of chlorpyrifos, malathion, and dimethoate by three strains of bacteria isolated from pesticide-polluted soils in sudan. | this study was done to identify pesticide-biodegrading microorganisms and to characterize degradation rates. bacillus safensis strain fo-36b(t), bacillus subtilis subsp. inaquosorum strain kctc13429(t), and bacillus cereus strain atcc14579(t) were isolated from pesticide-polluted soil in sudan, separately incubated with each pesticide with periodic samples drawn for gc and gc-ms. pesticide biodegradation followed a biphasic model. α and β half-lives (days) of chlorpyrifos, malathion, and dimetho ... | 2016 | 27771954 |
| partial characterization of bacteriocin-like compounds from two strains of bacillus cereus with biological activity against paenibacillus larvae, the causal agent of american foulbrood disease. | american foulbrood (afb), caused by the spore-forming gram-positive bacterium paenibacillus larvae, is the most severe bacterial disease affecting honeybees worldwide. two bacterial isolates showing specific inhibitory activity against p. larvae were identified as bacillus cereus by 16s rdna sequencing. antagonistic compounds were obtained from cell-free supernatants of strains m6c and m387 growing on trypticase soy broth and concentrated by nh4 so4 precipitation, ultrafiltration and butanol ext ... | 2016 | 27589675 |
| evolution of the microbiological profile of vacuum-packed ricotta salata cheese during shelf-life. | ricotta salata cheese is a salted variety of ricotta traditionally made in sardinia (italy) from the whey remaining after the production of pecorino romano protected designation of origin or other sheep milk cheeses. ricotta salata cheese is very critical for the possible growth of pathogenic and spoilage microorganisms. sporadic cases of listeriosis associated with ricotta salata cheese have been reported over recent years. the objective of the present study was to assess the evolution of spoil ... | 2016 | 27800440 |
| the structure of the pleiotropic transcription regulator cody provides insight into its gtp-sensing mechanism. | gtp and branched-chain amino acids (bcaas) are metabolic sensors that are indispensable for the determination of the metabolic status of cells. however, their molecular sensing mechanism remains unclear. cody is a unique global transcription regulator that recognizes gtp and bcaas as specific signals and affects expression of more than 100 genes associated with metabolism. herein, we report the first crystal structures of the full-length cody complex with sensing molecules and describe their fun ... | 2016 | 27596595 |
| cxcl1, but not il-6, significantly impacts intraocular inflammation during infection. | during intraocular bacterial infections, the primary innate responders are neutrophils, which may cause bystander damage to the retina or perturb the clarity of the visual axis. we hypothesized that cytokine il-6 and chemokine cxcl1 contributed to rapid neutrophil recruitment during bacillus cereus endophthalmitis, a severe form of intraocular infection that is characterized by explosive inflammation and retinal damage that often leads to rapid vision loss. to test this hypothesis, we compared e ... | 2016 | 27286792 |
| effect of surfactants and inducers on increased uricase production under submerged fermentations by bacillus cereus. | uricase is a clinical enzyme used for the oxidation of uric acid crystals in gout disease. the present study aimed to increase the suitable surfactant-mediated uricase production on induction by different concentrations of inducers. the efficiency of bacillus cereus to produce extracellular uricase enzyme was studied in uric acid-containing agar plates. among the studied inducers, uric acid is the potential inducer for uricase production under submerged fermentations (smf), which induced 19.41 u ... | 2017 | 27071031 |
| cotranscriptional folding of a riboswitch at nucleotide resolution. | rnas can begin to fold immediately as they emerge from rna polymerase. during cotranscriptional folding, interactions between nascent rnas and ligands are able to direct the formation of alternative rna structures, a feature exploited by noncoding rnas called riboswitches to make gene-regulatory decisions. despite their importance, cotranscriptional folding pathways have yet to be uncovered with sufficient resolution to reveal how cotranscriptional folding governs rna structure and function. to ... | 2016 | 27798597 |
| spovt: from fine-tuning regulator in bacillus subtilis to essential sporulation protein in bacillus cereus. | sporulation is a highly sophisticated developmental process adopted by most bacilli as a survival strategy to withstand extreme conditions that normally do not support microbial growth. a complicated regulatory cascade, divided into various stages and taking place in two different compartments of the cell, involves a number of primary and secondary regulator proteins that drive gene expression directed toward the formation and maturation of an endospore. such regulator proteins are highly conser ... | 2016 | 27790204 |
| new benzophenones and xanthones from cratoxylum sumatranum ssp. neriifolium and their antibacterial and antioxidant activities. | two new benzophenones (1 and 2) and four new xanthones (4-6 and 17) together with 24 known compounds (3, 7-16, and 18-30) were isolated from the roots and twigs of cratoxylum sumatranum ssp. neriifolium. their structures were elucidated by spectroscopic methods. compounds 5 and 26 showed antibacterial activity against micrococcus luteus, bacillus cereus, and staphylococcus epidermis with minimum inhibitory concentrations ranging from 4 to 8 μg/ml, whereas compounds 7, 20, and 26 displayed select ... | 2016 | 27788582 |
| co-precipitation synthesis and characterization of co doped sno2 nps, hsa interaction via various spectroscopic techniques and their antimicrobial and photocatalytic activities. | sn1-xcoxo2 (x=0.00, 0.01, 0.03, 0.05) nanoparticles (nps) of average size ∼30-40nm were synthesized by co-precipitation method. the interaction of co doped sno2 nps with human serum albumin (hsa) and their photocatalytic and antimicrobial properties were studied. the structural analysis and morphology of co doped sno2 nps were analysed via x-ray diffraction (xrd), scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (eds), high resolution transmission electron microscopy (hrt ... | 2017 | 27771412 |
| chemical variability and biological activities of essential oils of micromeria inodora (desf.) benth. from algeria. | the chemical composition of the essential oils isolated from the aerial parts of micromeria inodora (desf.) benth. collected in 24 algerian localities was investigated from the first time using gc-fid, gc/ms and (13) c-nmr. altogether, 83 components which accounted for 94.7% of the total oil composition were identified. the main compounds were trans-sesquisabinene hydrate (1; 20.9%), α-terpinyl acetate (2; 19.8%), globulol (3; 4.9%), caryophyllene oxide (4; 4.3%), β-bisabolol (5; 2.9%) and trans ... | 2016 | 27448034 |
| application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag. | we recently reported that the spore coat protein, cotb1 (171 amino acids), from bacillus cereus mediates silica biomineralization and that the polycationic c-terminal sequence of cotb1 (14 amino acids), designated cotb1p, serves as a silica-binding tag when fused to other proteins. here, we reduced the length of this silica-binding tag to only seven amino acids (sb7 tag: rqssrgr) while retaining its affinity for silica. alanine scanning mutagenesis indicated that the three arginine residues in t ... | 2016 | 27212265 |
| biosynthesis of palladium nanoparticles by using moringa oleifera flower extract and their catalytic and biological properties. | the biosynthesis of nanostructured biopalladium nanoparticles (pdnps) from an aqueous solution of crystalline palladium acetate is reported. for the synthesised pdnps in solution, an agroforest biomass waste petal of moringa oleifera derived bis-phthalate was used as natural reducing and biocapping agents. continuous absorption in the uv region and subsequent brown colour change confirmed the formation of pdnps. a strong surface plasmon peak for pdnps occurred at 460nm. pdnps were characterized ... | 2016 | 27776261 |
| itraq-based proteomic analysis of li-f type peptides produced by paenibacillus polymyxa jsa-9 mode of action against bacillus cereus. | li-f type peptides (amp-jsa9) produced by paenibacillus polymyxa jsa-9 are a group of cyclic lipodepsipeptide antibiotics that exhibit a broad antimicrobial spectrum against gram-positive bacteria and filamentous fungi, especially bacillus cereus and fusarium moniliforme. in this study, to better understand the antibacterial mechanism of amp-jsa9 against b. cereus, the ultrastructure of amp-jsa9-treated b. cereus cells was observed by both atomic force microscopy and transmission electron micros ... | 2017 | 27609309 |
| antibody binding studies reveal conformational flexibility of the bacillus cereus non-hemolytic enterotoxin (nhe) a-component. | the non-hemolytic enterotoxin complex (nhe) is supposed to be the main virulence factor of b. cereus causing a diarrheal outcome of food poisoning. this tripartite toxin consists of the single components nhea, -b and -c all of them being necessary for maximum toxicity. in the past, research activities aiming to elucidate the mode-of-action of nhe were mostly focused on the b- and c-component. in this study the generation of novel monoclonal antibodies (mab) and their thorough characterization en ... | 2016 | 27768742 |
| the heat resistance and spoilage potential of aerobic mesophilic and thermophilic spore forming bacteria isolated from chinese milk powders. | the propensity for aerobic bacilli and allied genera to produce highly heat-resistant spores and thermally stable spoilage enzymes are major bacteriological issues faced by the dairy industry. most of the enzymes are able to survive any heat treatment applied during the manufacture of milk powders and have the potential to remain active in milk powders and other dairy products during storage, and may explain some of the sensory and functionality defects reported in dairy products. despite many r ... | 2016 | 27657656 |
| non-hemolytic enterotoxin of bacillus cereus induces apoptosis in vero cells. | bacillus cereus is an opportunistic pathogen that often causes foodborne infectious diseases and food poisoning. non-hemolytic enterotoxin (nhe) is the major toxin found in almost all enteropathogenic b. cereus and b. thuringiensis isolates. however, little is known about the cellular response after nhe triggered pore formation on cell membrane. here, we demonstrate that nhe induced cell cycle arrest at g0 /g1 phase and provoked apoptosis in vero cells, most likely associated with mitogen-activa ... | 2017 | 27762484 |
| synthesis and antibacterial evaluation of new n- and s-glycosides analogues with dinitrophenyl-substituted heterocyclic bases. | four n- and s-glycosides 13-16 having nucleobases 7-12 binding to sugar molecules from one side and to 3,5-dinitrophenyl moieties from another side were synthesized from 3,5-dinitrobenzoic acid 2. the synthetic intermediates, hydrazide 5 and thiosemicarbazide 6 regarded as important key compounds for the synthesis of nucleobases 7-12, each was obtained by two approaches. structures of synthesized compounds were determined spectroscopically. antibacterial activities for synthetic intermediates an ... | 2017 | 27761763 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| genome sequence of bacillus cereus forc_021, a food-borne pathogen isolated from a knife at a sashimi restaurant. | bacillus cereus causes food-borne illness through contaminated foods; therefore, its pathogenicity and genome sequences have been analyzed in several studies. we sequenced and analyzed b. cereus strain forc_021 isolated from a sashimi restaurant. the genome sequence consists of 5,373,294 bp with 35.36% gc contents, 5,350 predicted cdss, 42 rrna genes, and 107 trna genes. based on in silico dna-dna hybridization values, b. cereus atcc 14579(t) was closest to forc_021 among the complete genome-seq ... | 2016 | 27728961 |
| feasible management of southern corn leaf blight via induction of systemic resistance by bacillus cereus c1l in combination with reduced use of dithiocarbamate fungicides. | dithiocarbamate fungicides such as maneb and mancozeb are widely used nonsystemic protectant fungicides to control various plant fungal diseases. dithiocarbamate fungicides should be frequently applied to achieve optimal efficacy of disease control and avoid either decline in effectiveness or wash-off from leaf surface. dithiocarbamates are of low resistance risk but have the potential to cause human neurological diseases. the objective of this study was to develop a strategy to effectively cont ... | 2016 | 27721698 |
| bacillus cereus causing intratumoral brain abscess. | we report a case of intratumoral brain abscess due to bacillus cereus in an adult male patient, which was managed successfully with excision of lesion and piperacillin-tazobactam for the duration of 5 weeks. to the best of our knowledge, this is a first case report of b. cereus infection leading to intratumoral brain abscess in a patient with a history of steroid administration by the intravenous route. | 2017 | 27721298 |
| biochemical and biological characterization of two brassicaceae after their commercial expiry date. | two brassicaceae (eruca sativa, brassica oleracea var. sabauda) were stored in air and under a modified atmosphere for several days after their expiry date and then analyzed. the polyphenol content and composition, as well as the antioxidant activity of the extracts, were assessed, compared to the fresh products. antimicrobial properties on tester strains (bacillus cereus, escherichia coli, staphylococcus aureus and pseudomonas aeruginosa) and in vitro anti-proliferative activity were evaluated. ... | 2017 | 27719918 |
| the thnr gene is a negative transcription regulator of the thurincin h genetic cassette in bacillus thuringiensis subsp. morrisoni. | thurincin h is a bacteriocin synthesized by some strains of bacillus thuringiensis. in this study, the thurincin h genetic cassette, which contains ten genes, from a mexican strain of b. thuringiensis subsp. morrisoni (btm) was cloned and sequenced. to study the function of the thnr gene component in the cassette, we generated various constructs with or without thnr for expression in btm. all transformants harboring thnr in recombinant plasmids showed a decrease of ~15 to ~90 % in inhibitory act ... | 2017 | 27699438 |
| the comparing of antimicrobial activity of csn1s2 protein of fresh milk and yoghurt goat breed ethawah inhibited the pathogenic bacteria. | goat milk is reported to have antimicrobial activity of several pathogen bacteria that contained on food materials. the research related with antimicrobial activity of alpha-s2 casein from goat milk is relatively less than other casein components. herein, we reported the antimicrobial activity of caprine alpha-s2 casein (csn1s2) protein from ethawah breed goat milk and yoghurt in gram positive (listeria monocytogenes, staphylococcus aureus and bacillus cereus) and negative pathogen bacteria (esc ... | 2016 | 27698594 |
| draft genome sequence of bacillus cereus lcr12, a plant growth-promoting rhizobacterium isolated from a heavy metal-contaminated environment. | bacillus cereus lcr12 is a plant growth-promoting rhizobacterium, isolated from a heavy metal-contaminated environment. the 6.01-mb annotated genome sequence provides the genetic basis for revealing its potential application to remediate contaminated soils in association with plants. | 2016 | 27688340 |
| genome sequence of bacillus cereus group phage salinjah. | the double-stranded dna (dsdna) myoviridae bacillus cereus group bacteriophage salinjah was isolated from soil collected in gyeonggi-do, south korea. salinjah, a cluster c phage with a broad host range, suggests the need to create a new subcluster with salinjah and helga as founding members. | 2016 | 27688335 |
| bioactivity of a novel glycolipid produced by a halophilic buttiauxella sp. and improving submerged fermentation using a response surface method. | an antimicrobial glycolipid biosurfactant (gbs), extracted and identified from a marine bacterium, was studied to inhibit pathogenic microorganisms. production of the gbs was optimized using a statistical method, a response surface method (rsm) with a central composite design (ccd) for obtaining maximum yields on a cost-effective substrate, molasses. the gbs-producing bacterium was identified as buttiauxella species in terms of biochemical and molecular characteristics. this compound showed a de ... | 2016 | 27669197 |
| commercial disinfectants during disinfection process validation: more failures than success. | disinfection process validation is mandatory before introduction of a new disinfectant in hospital services. commercial disinfection brands often question existing hospital policy claiming greater efficacy and lack of toxicity of their products. inadvertent inadequate disinfection leads to morbidity, patient's economic burden, and the risk of mortality. | 2016 | 27656441 |
| chemical composition and in vitro antimicrobial, cytotoxic, and central nervous system activities of the essential oils of citrus medica l. cv. 'liscia' and c. medica cv. 'rugosa' cultivated in southern italy. | citrus medica cv. 'liscia' and c. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of south italy, in particular of amalfi coast, in the campania region. the chemical composition of the essential oils (eos) from fruit peels of both c. medica cultivars was studied by gas chromatography (gc) and gas chromatography-mass spectrometry (gc-ms) analyses. in all, 100 compounds were identified, 82 for c. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for c. me ... | 2016 | 27649138 |
| chemical composition, antibacterial and phytotoxic activities of peganum harmala seed essential oils from five different localities in northern africa. | peganum harmala l., also known as syrian rue or pègano, is a herbaceous plant belonging to the zygohpyllaceae family, and is widely used in traditional medicine. the chemical composition of essential oils of p. harmala seeds from five different regions of northern africa (algeria, egypt, libya, morocco and tunisia) was studied by gc and gc-ms analyses. a total of 105 compounds were identified, the main components being oxygenated monoterpenes and oxygenated sesquiterpenes. eugenol is the main co ... | 2016 | 27649128 |
| pellino-1 derived cationic antimicrobial prawn peptide: bactericidal activity, toxicity and mode of action. | the antimicrobial peptides (amps) are multifunctional molecules which represent significant roles in the innate immune system. these molecules have been well known for decades because of their role as natural antibiotics in both invertebrates and vertebrates. the development of multiple drug resistance against conventional antibiotics brought a greater focus on amps in recent years. the cationic peptides, in particular, proven as host defense peptides and are considered as effectors of innate im ... | 2016 | 27648859 |
| chemical composition and in vitro evaluation of antimicrobial, antioxidant and antigerminative properties of the seed oil from the tunisian endemic ferula tunetana pomel ex batt. | essential oil of the seeds from the endemic tunisian plant ferula tunetana pomel ex batt. was analyzed for its chemical composition and screened for its antimicrobial, antioxidant and antigerminative properties. the chemical composition of the isolated oil is reported for the first time. according to the gc/fid, gc/ms and (13) c-nmr analysis results; 18 compounds were identified accounting for 84.6% of the total oil. the chemical composition of this essential oil was characterized by the presenc ... | 2017 | 27638478 |
| possession, use, and transfer of select agents and toxins-- addition of bacillus cereus biovar anthracis to the hhs list of select agents and toxins. interim final rule and request for comments. | the centers for disease control and prevention (cdc) in the department of health and human services (hhs) is adding bacillus cereus biovar anthracis to the list of hhs select agents and toxins as a tier 1 select agent. we are taking this action to regulate this agent that is similar to b. anthracis to prevent its misuse, which could cause a biological threat to public health and/or national security. | 2016 | 27632805 |
| differentiation of foodborne bacteria using nir hyperspectral imaging and multivariate data analysis. | the potential for near-infrared (nir) hyperspectral imaging and multivariate data analysis to be used as a rapid non-destructive tool for detection and differentiation of bacteria was investigated. nir hyperspectral images were collected of bacillus cereus, escherichia coli, salmonella enteritidis, staphylococcus aureus and staphylococcus epidermidis grown on agar for 20 h at 37 °c. principal component analysis (pca) was applied to mean-centred data. standard normal variate (snv) correction and ... | 2016 | 27624097 |
| termitarium-inhabiting bacillus endophyticus tsh42 and bacillus cereus tsh77 colonizing curcuma longa l.: isolation, characterization, and evaluation of their biocontrol and plant-growth-promoting activities. | bacillus strains were isolated from termitarium soil and screened for their antifungal activity through the production of diffusible and volatile metabolites. further, the bacterial strains that showed antifungal activity were evaluated for their biocontrol potential on the basis of their plant-growth-promoting attributes. termitarium-inhabiting bacillus strains tsh42 and tsh77 significantly reduced the growth of pathogenic fungus fusarium solani, controlled the symptoms of rhizome rot in turmer ... | 2016 | 27604298 |
| the comparative analysis of antioxidant and biological activity for the dendropanax morbifera lev. leaves extracted by different ethanol concentrations. | the different concentrations of ethanol (20-100%) and distilled water extract for dendropanax morbifera lev. leaves were evaluated to induce antioxidant and biological activity employed by variety of assays. the 20%, 80%, and 100% ethanol extract demonstrated the relatively higher activity, whereas distilled water, 40%, and 60% ethanol extracts exhibited the lower antioxidant and biological activity. especially, 80% ethanol extract showed the remarkably higher radical scavenging activity, reduci ... | 2016 | 27592831 |
| genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing bacillus cereus. | in this study, the efficiency of free and immobilized cells of newly isolated hexavalent chromium [cr(vi)] reducing bacillus cereus strain cr1 (accession no. kj162160) was studied in the treatment of tannery effluent. the analysis of effluents revealed high chemical oxygen demand (cod-1260 mg/l), biological oxygen demand (bod5-660 mg/l), total dissolved solids (tds-14000 mg/l), electrical conductivity (ec-21.5 ms/cm) and total chromium (tc-2.4 mg/l). the effluents also showed genotoxic effects t ... | 2016 | 27591849 |
| draft genome sequence of biocontrol agent bacillus cereus uw85. | bacillus cereus uw85 was isolated from a root of a field-grown alfalfa plant from arlington, wi, and identified for its ability to suppress damping off, a disease caused by phytophthora megasperma f. sp. medicaginis on alfalfa. here, we report the draft genome sequence of b. cereus uw85, obtained by a combination of sanger and illumina sequencing. | 2016 | 27587823 |
| bacterial succession and metabolite changes during flax (linum usitatissimum l.) retting with bacillus cereus hdym-02. | high-throughput sequencing and gc-ms (gas chromatography-mass spectrometry) were jointly used to reveal the bacterial succession and metabolite changes during flax (linum usitatissimum l.) retting. the inoculation of bacillus cereus hdym-02 decreased bacterial richness and diversity. this inoculum led to the replacement of enterobacteriaceae by bacillaceae. the level of aerobic pseudomonadaceae (mainly azotobacter) and anaerobic clostridiaceae_1 gradually increased and decreased, respectively. f ... | 2016 | 27585559 |
| factors of detection of bacillus cereus strains in eye cream. | bacillus cereus has been associated with clinical infections and is also the cause of post-traumatic endophthalmitis as well as endogenous eye infections, which can result in blindness. cosmetics, although preserved, can be contaminated during manufacture or use and thus cause serious health issues. | 2017 | 27578165 |
| identification of pyridine synthase recognition sequences allows a modular solid-phase route to thiopeptide variants. | thiopeptides are structurally complex, bioactive natural products derived from ribosomally synthesized and post-translationally modified peptides. a remarkable set of enzymes were recently revealed to catalyze the formation of the core trithiazolylpyridine of thiopeptides via a formal [4 + 2] cycloaddition. these pyridine synthases typically act late in thiopeptide biosynthesis to affect macrocyclization and cleavage of the n-terminal leader peptide, making them potentially useful biocatalysts f ... | 2016 | 27575591 |
| teaching neuroimages: diffuse cerebral vasospasm and multiple intracranial abscesses from bacillus cereus. | | 2016 | 27572436 |
| a catalytic role for c-h/π interactions in base excision repair by bacillus cereus dna glycosylase alkd. | dna glycosylases protect genomic integrity by locating and excising aberrant nucleobases. substrate recognition and excision usually take place in an extrahelical conformation, which is often stabilized by π-stacking interactions between the lesion nucleobase and aromatic side chains in the glycosylase active site. bacillus cereus alkd is the only dna glycosylase known to catalyze base excision without extruding the damaged nucleotide from the dna helix. instead of contacting the nucleobase itse ... | 2016 | 27571247 |
| activation of the class ib ribonucleotide reductase by a flavodoxin reductase in bacillus cereus. | to reduce ribonucleotides to deoxyribonucleotides, the manganese-bound form of class ib ribonucleotide reductase (rnr) must be activated via a pathway that involves redox protein(s). the reduced flavoprotein nrdi is an important protein in this pathway, as it reduces dioxygen to superoxide. superoxide then reacts with the rnr mn(ii)2 site to generate a tyrosyl radical that is required for catalysis. a native nrdi reductase has not yet been identified. we herein demonstrate through kinetic and sp ... | 2016 | 27559930 |
| investigation on antimicrobial agents of the terrestrial streptomyces sp. bcc71188. | the terrestrial actinomycete strain bcc71188 was identified as streptomyces by its morphology (having spiral chain spore on the aerial mycelium), chemotaxonomy (containing ll-diaminopimelic acid in the cell wall), and 16s rrna gene sequence analysis [showing high similarity values compared with streptomyces samsunensis m1463(t) (99.85 %) and streptomyces malaysiensis nbrc 16446(t) (99.40 %)]. the crude extract exhibited antimalarial against plasmodium falciparum (ic50 0.19 μg/ml), anti-tb agains ... | 2017 | 27554496 |
| myrcia ovata cambessedes essential oils: a proposal for a novel natural antimicrobial against foodborne bacteria. | this paper reports the innovative antibacterial activity of essential oils (eos) from nine myrcia ovata cambessedes plants against eight foodborne bacteria. staphylococcus aureus, bacillus cereus, bacillus subtilis, enterococcus faecalis and pseudomonas aeruginosa were the most susceptible bacteria to eos. in particular, the p. aeruginosa, which is usually resistant to antimicrobials agents, was extremely sensitive to some eos. the gram-positive and gram-negative bacteria were inhibited and elim ... | 2016 | 27554278 |
| proteome data to explore the impact of pbclin15 on bacillus cereus atcc 14579. | this data article reports changes in the cellular and exoproteome of b. cereus cured from pbclin15.time-course changes of proteins were assessed by high-throughput nanolc-ms/ms. we report all the peptides and proteins identified and quantified in b. cereus with and without pbclin15. proteins were classified into functional groups using the information available in the kegg classification and we reported their abundance in term of normalized spectral abundance factor. the repertoire of experiment ... | 2016 | 27547804 |
| new insights into the interaction between the quorum-sensing receptor nprr and its dna target, or the response regulator spo0f. | the nprr protein and nprrb signaling peptide comprise a bifunctional quorum-sensing system from the bacillus cereus group that is involved in transcriptional activation through dna-binding and in sporulation initiation by binding to spo0f. we characterized in vitro the direct interactions established by nprr that may be relevant for performing its two functions. apo-nprr interacted with spo0f, but not with the target dna. the nprrb signaling peptide sskpdivg that binds strongly to apo-nprr, fail ... | 2016 | 27543719 |
| persistent bacillus cereus bacteremia in 3 persons who inject drugs, san diego, california, usa. | bacillus cereus is typically considered a blood culture contaminant; however, its presence in blood cultures can indicate true bacteremia. we report 4 episodes of b. cereus bacteremia in 3 persons who inject drugs. multilocus sequence typing showed that the temporally associated infections were caused by unrelated clones. | 2016 | 27533890 |
| mechanism of enhanced antibacterial activity of ultra-fine zno in phosphate buffer solution with various organic acids. | ultra-fine-zno showed low toxicity in complex water matrix containing multiple components such as pbs buffer and the toxic mechanism of ultra-fine-zno has not been clearly elucidated. in present study, enhanced antibacterial activity of 200 nm diameter ultra-fine-zno in pbs buffer against bacillus cereus and escherichia coli were observed in the presence of several organic acids in comparison with ultra-fine-zno in pbs buffer alone. these findings indicated that the toxic effects of the ultra-fi ... | 2016 | 27524254 |
| bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic bacillus cereus group species isolated from dairy foods and dairy environments. | a facultatively anaerobic, spore-forming bacillus strain, fsl w8-0169t, collected from raw milk stored in a silo at a dairy powder processing plant in the north-eastern usa was initially identified as a bacillus cereus group species based on a partial sequence of the rpob gene and 16s rrna gene sequence. analysis of core genome single nucleotide polymorphisms clustered this strain separately from known b. cereus group species. pairwise average nucleotide identity blast values obtained for fsl w8 ... | 2016 | 27520992 |
| potential risk of acute toxicity induced by agi cloud seeding on soil and freshwater biota. | silver iodide is one of the most common nucleating materials used in cloud seeding. previous cloud seeding studies have concluded that agi is not practically bioavailable in the environment but instead remains in soils and sediments such that the free ag amounts are likely too low to induce a toxicological effect. however, none of these studies has considered the continued use of this practice on the same geographical areas and thus the potential cumulative effect of environmental agi. the aim o ... | 2016 | 27517140 |
| evaluation of health potential of nutritionally enriched kodo millet (eleusine coracana) grown in himachal pradesh, india. | in this study, kodo millet grains were phytochemically investigated for their nutritional and antioxidant potential for their use as functional foods. methanolic extracts of grains showed higher phenolic content and antioxidant activity. tlc studies of the extracted polyphenols from kodo millet showed the predominant presence of ferulic acid and cinnamic acid in the millet. further quantification of these polyphenols was done by using hplc, analysing ferulic acid and cinnamic acid. antagonistic ... | 2017 | 27507461 |
| production of hemolysin bl by bacillus cereus group isolates of dairy origin is associated with whole-genome phylogenetic clade. | bacillus cereus group isolates that produce diarrheal or emetic toxins are frequently isolated from raw milk and, in spore form, can survive pasteurization. several species within the b. cereus group are closely related and cannot be reliably differentiated by established taxonomical criteria. while b. cereus is traditionally recognized as the principal causative agent of foodborne disease in this group, there is a need to better understand the distribution and expression of different toxin and ... | 2016 | 27507015 |
| degradation of acephate by enterobacter asburiae, bacillus cereus and pantoea agglomerans isolated from diamondback moth plutella xylostella (l), a pest of cruciferous crops. | acephate-degrading bacterial isolates were isolated from the larval gut of diamondback moth plutella xylostella, a notorious pest of cruciferous crops worldwide that has developed resistance to insecticides. partial 16s rrna gene sequencing identified the isolates as bacillus cereus (px-b.c.or), enterobacter asburiae (pxe), and pantoae agglomerans (px-pt.ag.jor). all isolates grew on minimal media (mm) in the presence of acephate at 100 and 200 ppm, with maximum growth at 200 ppm. lc-ms analyses ... | 2016 | 27498509 |
| comparative evaluation of cyanide removal by adsorption, biodegradation, and simultaneous adsorption and biodegradation (sab) process using bacillus cereus and almond shell. | the present study aimed to investigate the removal efficiency of cyanide from contaminated water by adsorption, biodegradation and simultaneous adsorption and biodegradation (sab) process individually in a batch reactor. adsorption was achieved by using almond shell granules and biodegradation was conducted with suspended cultures of bacillus cereus, whereas sab process was carried out using bacillus cereus and almond shell in a batch reactor. the effect of agitation time, ph, and initial cyanid ... | 2016 | 27498500 |
| simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods. | capillary electrophoresis-single strand conformation polymorphism (ce-sscp) coupled with stuffer-free multiplex ligation-dependent probe amplification (mlpa) was developed to identify 13 species of foodborne pathogens simultaneously. species-specific mlpa probes were designed for nine of these species. these probes were targeted to the groel, glya, mms, tuf, inv, ipah, nuc, vvh, and 16s rrna genes, which corresponded to bacillus cereus, campylobacter coli, cronobacter sakazakii, enterococcus spp ... | 2016 | 27494415 |
| design, synthesis, anticancer, antimicrobial activities and molecular docking studies of theophylline containing acetylenes and theophylline containing 1,2,3-triazoles with variant nucleoside derivatives. | a new series of theophylline containing acetylene derivatives (6a-6b and 7-13) and theophylline containing 1,2,3-triazoles with variant nucleoside derivatives (20-32) have been designed and synthesized. these compounds were screened for anticancer and antimicrobial activity. further the computational docking and 2d qsar were performed using moe software to identify novel scaffolds. the results showed that compound 29 and 30 exhibit significant cytotoxic effect on all four cancer cells such as lu ... | 2016 | 27487568 |
| recovery of heat treated bacillus cereus spores is affected by matrix composition and factors with putative functions in damage repair. | the ability of spores to recover and grow out after food processing is affected by cellular factors and by the outgrowth conditions. in the current communication we studied the recovery and outgrowth of individually sorted spores in bhi and rice broth media and on agar plates using flow cytometry. we show that recovery of wet heat treated bacillus cereus atcc 14579 spores is affected by matrix composition with highest recovery in bhi broth or on rice agar plates, compared to bhi agar plates and ... | 2016 | 27486443 |
| hexane extract of echinops spinosissimus turra subsp. spinosus from tunisia: a potential source of acetylated sterols - investigation of its biological activities. | the hexane extract of echinops spinosissimus turra subsp. spinosus flower heads was analyzed for its fatty acid and sterol composition. its physicochemical characteristics were also studied. the saponification, iodine and peroxide values were determined as 255 mg koh/g, 42.57 g i2 /100 g and 110 m equiv. o2 /kg of oil, respectively. the oleic (c18:1; 61.14%), palmitic (c16:0; 21.36%) and linoleic (c18:2; 10.45%) acids were the dominant fatty acids. this extract was also found to contain high lev ... | 2016 | 27476999 |
| development of an efficient bacterial consortium for the potential remediation of hydrocarbons from contaminated sites. | the intrinsic biodegradability of hydrocarbons and the distribution of proficient degrading microorganisms in the environment are very crucial for the implementation of bioremediation practices. among others, one of the most favorable methods that can enhance the effectiveness of bioremediation of hydrocarbon-contaminated environment is the application of biosurfactant producing microbes. in the present study, the biodegradation capacities of native bacterial consortia toward total petroleum hyd ... | 2016 | 27471499 |
| changes in microbial composition and the prevalence of foodborne pathogens in crab marinated in soy sauce produced by six manufacturing plants. | the present study examined the changes in microbiological composition during the production process of crab marinated in soy sauce, potential microbial hazards, potential contamination routes and effective critical control points. crab and soy sauce samples were obtained from six different manufacturing plants at different stages, and their microbiological content was comprehensively assessed by quantitative and qualitative analyses. | 2017 | 27469473 |
| enhanced efficacy of clindamycin hydrochloride encapsulated in pla/plga based nanoparticle system for oral delivery. | clindamycin hydrochloride (clh) is a clinically important oral antibiotic with wide spectrum of antimicrobial activity that includes gram-positive aerobes (staphylococci, streptococci etc.), most anaerobic bacteria, chlamydia and certain protozoa. the current study was focused to develop a stabilised clindamycin encapsulated poly lactic acid (pla)/poly (d,l-lactide-co-glycolide) (plga) nano-formulation with better drug bioavailability at molecular level. various nanoparticle (nps) formulations o ... | 2016 | 27463797 |
| prodigiosin inhibits motility and activates bacterial cell death revealing molecular biomarkers of programmed cell death. | the antimicrobial activity of prodigiosin from serratia nematodiphila darsh1, a bacterial pigment was tested against few food borne bacterial pathogens bacillus cereus, staphylococcus aureus, pseudomonas aeruginosa and escherichia coli. the mode of action of prodigiosin was studied. prodigiosin induced bactericidal activity indicating a stereotypical set of biochemical and morphological feature of programmed cell death (pcd). pcd involves dna fragmentation, generation of ros, and expression of a ... | 2016 | 27460563 |
| lactobacillus delbrueckii subsp lactis cidca 133 modulates response of human epithelial and dendritic cells infected with bacillus cereus. | it is known that probiotic microorganisms are able to modulate pathogen virulence. this ability is strain dependent and involves multiple interactions between microorganisms and relevant host's cell populations. in the present work we focus on the effect of a potentially probiotic lactobacillus strain (lactobacillus delbrueckii subsp. lactis cidca 133) in an in vitro model of bacillus cereus infection. our results showed that infection of intestinal epithelial ht-29 cells by b. cereus induces nu ... | 2016 | 27459335 |
| chemical composition and biological activity of centaurea baseri: new species from turkey. | the genus centaurea l. is one of the largest and important genera of asteraceae family. centaurea species have been widely used as herbal remedies in folk medicine for their antidandruff, antidiarrheic, antirheumatic, anti-inflammatory, choleretic, diuretic, digestive, stomachic, astringent, antipyretic, cytotoxic, and antibacterial properties. centaurea baseri kose & alan is a recently described local endemic species in turkey and this is the first study on the chemical composition and bioactiv ... | 2016 | 27447348 |
| the comer gene plays an important role in biofilm formation and sporulation in both bacillus subtilis and bacillus cereus. | bacteria adopt alternative cell fates during development. in bacillus subtilis, the transition from planktonic growth to biofilm formation and sporulation is controlled by a complex regulatory circuit, in which the most important event is activation of spo0a, a transcription factor and a master regulator for genes involved in both biofilm formation and sporulation. in b. cereus, the regulatory pathway controlling biofilm formation and cell differentiation is much less clear. in this study, we sh ... | 2016 | 27446060 |
| expression of the genes encoding the cask/r two-component system and the desa desaturase during bacillus cereus cold adaptation. | two-component systems (tcs) allow a cell to elaborate a variety of adaptive responses to environment changes. the recently discovered cask/r tcs plays a role in the optimal unsaturation of fatty acids necessary for cold adaptation of the foodborne-pathogen bacillus cereus here, we showed that the promoter activity of the operon encoding this tcs was repressed during growth at low temperature in the stationary phase in the parental strain when compared to the cask/r mutant, suggesting that casr n ... | 2016 | 27435329 |
| evolutionary processes and environmental factors underlying the genetic diversity and lifestyles of bacillus cereus group bacteria. | bacteria of the bacillus cereus group are environmental gram-positive spore-forming bacteria ubiquitously distributed. despite the high degree of genetic similarity among the different strains, they show strong phenotypic variability, from mammal or entomopathogen strains to soil-dwelling saprophytes, and from psychrophylic to thermotolerant strains. most of the phenotypes are linked to the presence of large plasmids that encode for diverse toxins. however, other processes, like mutation or reco ... | 2016 | 27424810 |
| bacillus spore wet heat resistance and evidence for the role of an expanded osmoregulatory spore cortex. | previous work reported that decoated bacillus cereus spores incubated in 4 mol l(-1) cacl2 are killed at lower temperatures than spores in water. this wet heat sensitization was suggested to support a role for an osmoregulatory peptidoglycan cortex in spore cores' low water content, and their wet heat resistance. current work has replicated this finding with spores of b. cereus, bacillus megaterium and bacillus subtilis. however, this work found that decoated spores apparently killed at 80°c in ... | 2016 | 27424522 |
| complete genome sequences of nine bacillus cereus group phages. | we report the sequences of nine novel bacillus cereus group bacteriophages: dignkc, juglone, nemo, nigalana, notthecreek, phrodo, sagefayge, vinny, and zuko. these bacteriophages are double-stranded dna-containing myoviridae isolated from soil samples using b. thuringiensis subsp. kurstaki as the host bacterium. | 2016 | 27417827 |
| [functional analysis of grp and iris, the gag and env domesticated errantivirus genes, in the drosophila melanogaster genome]. | drosophila melanogaster is the only invertebrate that contains endogenous retroviruses, which are called errantiviruses. two domesticated genes, grp and iris, which originate from errantivirus gag and env, respectively, have been found in the d. melanogaster genome. the functions performed by the genes in drosophila are still unclear. to identify the functions of domesticated gag and env in the d. melanogaster genome, expression of iris and grp was studied in strains differing by the presence or ... | 2017 | 27414781 |
| bacteriocinogenic bacteria isolated from raw goat milk and goat cheese produced in the center of méxico. | currently, there are few reports on the isolation of microorganisms from goat milk and goat cheese that have antibacterial activity. in particular, there are no reports on the isolation of microorganisms with antibacterial activity from these products in central mexico. our objective was to isolate bacteria, from goat products, that synthesized antimicrobial peptides with activity against a variety of clinically significant bacteria. we isolated and identified lactobacillus rhamnosus, l. plantar ... | 2016 | 27407294 |
| integrative approach for producing hydrogen and polyhydroxyalkanoate from mixed wastes of biological origin. | in this study, an integrative approach to produce biohydrogen (h2) and polyhydroxyalkanoates (pha) from the wastes of biological origin was investigated. a defined set of mixed cultures was used for hydrolysis and the hydrolysates were used to produce h2. the effluent from h2 production stage was used for pha production. under batch culture, a maximum of 62 l h2/kg of pure potato peels (total solid, ts 2 %, w/v) and 54 l h2/kg of mixed biowastes (mbw1) was recorded. using effluent from the h2 pr ... | 2016 | 27407293 |
| low-cost and eco-friendly green synthesis of silver nanoparticles using prunus japonica (rosaceae) leaf extract and their antibacterial, antioxidant properties. | low cost and eco-friendly green synthesis of silver nanoparticles (agnps) from silver nitrate (agno3) using prunus japonica leaves extract as reducing agent by a simple method at room temperature. the biosynthesized nanoparticles (nps) were characterized by uv-vis, tunneling electron microscopy (hr-tem), scanning electron microscopy (sem) coupled with x-ray energy dispersive spectrophotometer (edax), x-ray diffraction (xrd) and fourier transform infrared spectroscopy (ftir). in uv-vis spectrosco ... | 2016 | 27396523 |
| common mechanism of cross-resistance development in pathogenic bacteria bacillus cereus against alamethicin and pediocin involves alteration in lipid composition. | to understand the mechanism of development of cross-resistance in food pathogen bacillus cereus against an antimicrobial peptide pediocin and antibiotic alamethicin, the present study was designed. pediococcus pentosaceus was taken as a source of pediocin, and it was purified by ammonium sulphate precipitation followed by cation exchange chromatography with 14.01-fold purity and 14.4 % recovery. b. cereus strains alamethicin-resistant strains (ic50 3.23 µg/ml) were selected from sensitive popula ... | 2016 | 27378130 |
| comparative genomics of iron-transporting systems in bacillus cereus strains and impact of iron sources on growth and biofilm formation. | iron is an important element for bacterial viability, however it is not readily available in most environments. we studied the ability of 20 undomesticated food isolates of bacillus cereus and two reference strains for capacity to use different (complex) iron sources for growth and biofilm formation. studies were performed in media containing the iron scavenger 2,2-bipyridine. transcriptome analysis using b. cereus atcc 10987 indeed showed upregulation of predicted iron transporters in the prese ... | 2016 | 27375568 |
| combined effect of anaerobiosis, low ph and cold temperatures on the growth capacities of psychrotrophic bacillus cereus. | psychrotrophic strains of the foodborne pathogen bacillus cereus can multiply during the refrigerated storage of food products. the aim of this study was to determine the impact of anaerobiosis on the growth of two psychrotrophic b. cereus strains exposed to acidic ph at a cold temperature in a laboratory medium. at 10 °c, growth occurred at ph values equal to or higher than 5.7 during anaerobiosis, whereas aerobic growth was observed from ph 5.4. growth rates during aerobiosis were similar at p ... | 2016 | 27375252 |
| effect of endophytic bacillus cereus erbp inoculation into non-native host: potentials and challenges for airborne formaldehyde removal. | phytoremediation could be a cost-effective, environmentally friendly approach for the treatment of indoor air. however, some drawbacks still dispute the expediency of phytotechnology. our objectives were to investigate the competency of plant growth-promoting (pgp) endophytic bacillus cereus erbp (endophyte root blue pea), isolated from the root of clitoria ternatea, to colonize and stabilize within zamioculcas zamiifolia and euphorbia milii as non-native hosts without causing any disease or str ... | 2016 | 27362296 |
| genetic and functional analyses of krs, a locus encoding kurstakin, a lipopeptide produced by bacillus thuringiensis. | bacteria of the bacillus genus are able to synthesize several families of lipopeptides. these small molecules are the product of non-ribosomal peptide synthetases. in 2000, it was found that bacillus thuringiensis, an entomopathogenic bacterium of the bacillus cereus group, produced a previously unknown lipopeptide: kurstakin. genomic analyses reveal that the krs locus, encoding the kurstakin synthetases, is specific to the b. cereus group, but is unevenly distributed within this group. previous ... | 2016 | 27353188 |
| modulation of phytoremediation and plant growth by the treatment with pgpr, ag nanoparticle and untreated municipal wastewater. | the present attempt was made to determine the effects of untreated municipal wastewater (mw) on growth and physiology of maize and to evaluate the role of ag nanoparticle and plant-growth-promoting rhizobacteria (pgpr) when interacting with mw used for irrigation. it was used for the isolation of pgpr. the isolates were identified and characterized based on the colony morphology, c/n source utilization pattern using miniaturized identification system (qts 24), catalase (cat) and oxidase tests, a ... | 2016 | 27348506 |
| photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity. | we present the simple, eco-friendly synthesis of silver nanoparticles (agnps) using sunlight or green, red, blue, or white led light together with dryopteris crassirhizoma rhizome extract (dcre) as the reducing and capping agent. the preliminary indication of agnp production was a color change from yellowish green to brown after light exposure in the presence of dcre. optimization of parameters such as ph, inoculum dose, and metal ion concentration played an important role in achieving nanoparti ... | 2016 | 27348063 |
| chemical profile, antioxidant and antibacterial activities of achillea moschata wulfen, an endemic species from the alps. | aerial parts of achillea moschata wulfen (asteraceae) growing wild in the italian rhaetian alps were investigated to describe, for the first time, their phenolic content, as well as to characterize the essential oil. inspection of the metabolic profile combining hplc-dad and esi-ms/ms data showed that the methanol extract contained glycosylated flavonoids with luteolin and apigenin as the main aglycones. among them, the major compound was 7-o-glucosyl apigenin. caffeoyl derivates were other phen ... | 2016 | 27347915 |
| microbiological isolates and antibiotic susceptibilities: a 10-year review of culture-proven endophthalmitis cases. | to review the microbiological spectrum and antibiotic sensitivities of the pathogens that cause culture-proven endophthalmitis and to understand the status and trends of antibiotic susceptibility at a public hospital over a 10-year period. | 2017 | 27347700 |