| a double-blind randomized trial comparing implants with laser-microtextured and machined collar surfaces: microbiologic and clinical results. | the purpose of this study was to compare the clinical outcome of and determine the differences in periodonto-pathogenic microbiota around two types of implant collar surfaces: laser-microtextured (test) vs machined (control). | 2017 | 27632268 |
| the distribution of porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans in patients with alcoholic disease: a pilot study. | both drinking and periodontal disease are serious health and social problems. findings on the effect of alcohol consumption on periodontal disease are inconclusive. | 2016 | 27627556 |
| mannose-binding lectin gene polymorphism in relation to periodontal infection. | mannose-binding lectin (mbl) plays an important role in innate immunity. mbl deficiency is usually caused by mutations in exon 1 of the mbl structural gene (mbl2). our aim was to investigate mbl2 polymorphisms and their relation to salivary levels of periodontal inflammatory/tissue destruction markers and two major periodontitis-associated bacteria. | 2016 | 27624657 |
| virulence factors associated with aggregatibacter actinomycetemcomitans and their role in promoting periodontal diseases. | | 2017 | 27623711 |
| herpesviral-bacterial co-infection in mandibular third molar pericoronitis. | the aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis. | 2016 | 27620215 |
| role of nod2 and rip2 in host-microbe interactions with gram-negative bacteria: insights from the periodontal disease model. | nod2 is a member of the nlr family of proteins that participate in the activation of the innate immune response. rip2 is a downstream kinase activated by both nod1 and nod2. there is scarcity of information regarding the relevance of nod2 in periodontitis, a chronic inflammatory condition characterized by inflammatory bone resorption. we used nod2-ko and rip2-ko mice in a model of microbial-induced periodontitis. heat-killed aggregatibacter actinomycetemcomitans was injected in the gingival tiss ... | 2016 | 27605548 |
| effect of helicobacter pylori infection on chronic periodontitis by the change of microecology and inflammation. | helicobacter pylori (h. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. most previous studies are case-controlled, and they investigate the risk of h. pylori infection in disease the development of while few studies evaluate the correlation between h. pylori and periodontal pathogens. therefore, we investigated the correlation between h. pylori infection with periodontal parameters, periodontal pathogens and inflammation. the results ... | 2016 | 27602578 |
| micrornas regulate cytokine responses in gingival epithelial cells. | micrornas (mirnas) have been established as key regulators of various biological processes with possible involvement in the pathobiology of periodontal disease. expanding our earlier observations of substantial differential expression of specific mirnas between clinically healthy and periodontitis-affected gingival tissues, we used mirna inhibitors (sponges) in loss-of-function experiments to investigate the involvement of specific mirnas in the response of pocket epithelium-derived, telomerase- ... | 2016 | 27600506 |
| detection of porphyromonas gingivalis and aggregatibacter actinomycetemcomitans after systemic administration of amoxicillin plus metronidazole as an adjunct to non-surgical periodontal therapy: a systematic review and meta-analysis. | to evaluate the variations in the detection of porphyromonas gingivalis and/or aggregatibacter actinomycetemcomitans before and after systemic administration of amoxicillin plus metronidazole in association with non-surgical periodontal therapy (nspt). | 2016 | 27594851 |
| adhesion of periodontal pathogens to self-ligating orthodontic brackets: an in-vivo prospective study. | our aims were to analyze adhesion of periodontopathogens to self-ligating brackets (clarity-sl [csl], clippy-c [cc] and damon q [dq]) and to identify the relationships between bacterial adhesion and oral hygiene indexes. | 2016 | 27585775 |
| novel bioactive tetracycline-containing electrospun polymer fibers as a potential antibacterial dental implant coating. | the purpose of this investigation was to determine the ability of tetracycline-containing fibers to inhibit biofilm formation of peri-implantitis-associated pathogens [i.e., porphyromonas gingivalis (pg), fusobacterium nucleatum (fn), prevotella intermedia (pi), and aggregatibacter actinomycetemcomitans (aa)]. tetracycline hydrochloride (tch) was added to a poly(dl-lactide) [pla], poly(ε-caprolactone) [pcl], and gelatin [gel] polymer blend solution at distinct concentrations to obtain the follow ... | 2016 | 27585669 |
| d-galactose as an autoinducer 2 inhibitor to control the biofilm formation of periodontopathogens. | autoinducer 2 (ai-2) is a quorum sensing molecule to which bacteria respond to regulate various phenotypes, including virulence and biofilm formation. ai-2 plays an important role in the formation of a subgingival biofilm composed mostly of gram-negative anaerobes, by which periodontitis is initiated. the aim of this study was to evaluate d-galactose as an inhibitor of ai-2 activity and thus of the biofilm formation of periodontopathogens. in a search for an ai-2 receptor of fusobacterium nuclea ... | 2016 | 27572513 |
| prevalence of periodontopathogens and candida spp. in smokers after nonsurgical periodontal therapy - a pilot study. | this pilot study aimed to evaluate the influence of smoking on clinical and microbiological parameters after nonsurgical periodontal therapy. forty-eight subjects were grouped into smokers (sm, n = 24) and nonsmokers (ns, n = 24) and paired according to gender, age, ethnicity, and periodontal status. both groups received oral hygiene education and scaling and root planing. clinical evaluation was performed using plaque index (pi), bleeding on probing (bop), pocket probing depth (ppd), gingival r ... | 2016 | 27556680 |
| longitudinal study on clinical and microbial analysis of periodontal status in pregnancy. | this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ... | 2016 | 27556678 |
| melanocortin agonism as a viable strategy to control alveolar bone loss induced by oral infection. | alveolar bone loss is a result of an aggressive form of periodontal disease (pd) associated with aggregatibacter actinomycetemcomitans (aa) infection. pd is often observed with other systemic inflammatory conditions, including arthritis. melanocortin peptides activate specific receptors to exert antiarthritic properties, avoiding excessing inflammation and modulating macrophage function. recent work has indicated that melanocortin can control osteoclast development and function, but whether such ... | 2016 | 27535487 |
| oxygen as a virulence determinant in polymicrobial infections. | infections caused by multiple organisms, or polymicrobial infections, are likely more common than is broadly appreciated. interaction among microbial communities (and with their host) can change the infection landscape by subverting immunity, providing nutrients and inhibiting competing microbes. stacy et al. (a. stacy, d. fleming, r. j. lamont, k. p. rumbaugh, and m. whiteley, mbio 7:e00782-16, 2016, http://dx.doi.org/10.1128/mbio.00782-16) described a novel mechanism that results in synergisti ... | 2016 | 27531913 |
| inhibition of p2x receptors protects human monocytes against damage by leukotoxin from aggregatibacter actinomycetemcomitans and α-hemolysin from escherichia coli. | α-hemolysin (hlya) from escherichia coli and leukotoxin a (ltxa) from aggregatibacter actinomycetemcomitans are important virulence factors in ascending urinary tract infections and aggressive periodontitis, respectively. the extracellular signaling molecule atp is released immediately after insertion of the toxins into plasma membranes and, via p2x receptors, is essential for the erythrocyte damage inflicted by these toxins. moreover, atp signaling is required for the ensuing recognition and ph ... | 2016 | 27528275 |
| systemic aggregatibacter actinomycetemcomitans leukotoxin-neutralizing antibodies in periodontitis. | leukotoxin (ltx) expressed by aggregatibacter actinomycetemcomitans is a powerful exotoxin, which can cause imbalance in host response. immunoreactivity to ltx is a marker for presence of leukotoxic a. actinomycetemcomitans, a presence that may modify the disease pattern of colonized individuals. the aim of the present study is to examine presence of systemic immunoreactivity to a. actionmycetemcomitans ltx with respect to clinical and inflammatory findings in individuals with or without periodo ... | 2017 | 27523521 |
| human serum-specific activation of alternative sigma factors, the stress responders in aggregatibacter actinomycetemcomitans. | aggregatibacter actinomycetemcomitans, a known pathogen causing periodontal disease and infective endocarditis, is a survivor in the periodontal pocket and blood stream; both environments contain serum as a nutrient source. to screen for unknown virulence factors associated with this microorganism, a. actinomycetemcomitans was grown in serum-based media to simulate its in vivo environment. different strains of a. actinomycetemcomitans showed distinct growth phenotypes only in the presence of hum ... | 2016 | 27490177 |
| transcriptome analysis of b cell immune functions in periodontitis: mucosal tissue responses to the oral microbiome in aging. | evidence has shown activation of t and b cells in gingival tissues in experimental models and in humans diagnosed with periodontitis. the results of this adaptive immune response are noted both locally and systemically with antigenic specificity for an array of oral bacteria, including periodontopathic species, e.g., porphyromonas gingivalis and aggregatibacter actinomycetemcomitans. it has been recognized through epidemiological studies and clinical observations that the prevalence of periodont ... | 2016 | 27486459 |
| detection of selected periodontal bacteria in preschool children affected by early childhood caries. | the aim of this study was to compare the detection frequency of periodontal bacteria in dental plaque in children with early childhood caries (ecc) with and without gingival inflammation. a convenience sample of 25 preschool children (mean age 3.61 years, sd 1.42) was recruited. dental plaque was taken from periodontal areas with and without visible signs of inflammation and processed using the stomagene® (protean s.r.o. czech republic) and parocheck® 20 (greiner bio-one gmbh, germany) detection ... | 2016 | 27485551 |
| oral pathogens change proliferation properties of oral tumor cells by affecting gene expression of human defensins. | the impact of oral pathogens onto the generation and variability of oral tumors has only recently been investigated. to get further insights, oral cancer cells were treated with pathogens and additionally, as a result of this bacterial cellular infection, with human defensins, which are as anti-microbial peptide members of the innate immune system. after cell stimulation, proliferation behavior, expression analysis of oncogenic relevant defensin genes, and effects on egfr signaling were investig ... | 2016 | 27481514 |
| nucleases from prevotella intermedia can degrade neutrophil extracellular traps. | periodontitis is an inflammatory disease caused by periodontal bacteria in subgingival plaque. these bacteria are able to colonize the periodontal region by evading the host immune response. neutrophils, the host's first line of defense against infection, use various strategies to kill invading pathogens, including neutrophil extracellular traps (nets). these are extracellular net-like fibers comprising dna and antimicrobial components such as histones, ll-37, defensins, myeloperoxidase, and neu ... | 2016 | 27476978 |
| an assessment of early colonisation of implant-abutment metal surfaces by single species and co-cultured bacterial periodontal pathogens. | numerous studies have proposed that smooth metal surfaces reduce initial bacterial attachment in the establishment of an early biofilm formation. however, these studies have largely examined single bacterial species, which are not always relevant as pathogens identified as initiators of inflammatory peri-implantitis. this study investigated the adherence of four periodontally-relevant bacterial species to implant and abutment surfaces in current clinical use. | 2016 | 27472955 |
| bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas. | the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ... | 2016 | 27469562 |
| evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study. | the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ... | 2016 | 27469559 |
| oxygen high level laser therapy is efficient in treatment of chronic periodontitis: a clinical and microbiological study using pcr analysis. | in periodontology, lasers have been suggested for the photodynamic therapy (pdt). such therapy can be defined as the inactivation of cells, microorganisms or molecules induced by light and not by heat. the aim of our study is to assess the effect of oxygen high-level laser therapy (ohllt) in removing all bacterial deposits on root or implant surface by means of mechanical instrumentation and laser irradiation. ohllt has two effects on targeted bacteria and tissues, decontamination and biostimula ... | 2016 | 27469554 |
| fluoride incorporation in high phosphate containing bioactive glasses and in vitro osteogenic, angiogenic and antibacterial effects. | to manufacture and assess bioactivity of low fluoride/high phosphate (low f(-)/high p2o5) bioglasses (bgs). then the effects of bg-conditioned medium on osteoblast-like cell behavior and bg particles on bactericidal activity were investigated. | 2016 | 27461261 |
| a novel intrinsically disordered outer membrane lipoprotein of aggregatibacter actinomycetemcomitans binds various cytokines and plays a role in biofilm response to interleukin-1β and interleukin-8. | intrinsically disordered proteins (idps) do not have a well-defined and stable 3-dimensional fold. some idps can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. a novel outer membrane lipoprotein, bacterial interleukin receptor i (bilri) of the opportunistic oral pathogen aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (il)-1β. because the amino aci ... | 2017 | 27459270 |
| quantifying implant-associated biofilms: comparison of microscopic, microbiologic and biochemical methods. | biofilm-associated infections pose severe problems in modern implant medicine. screening for new implant materials with antibacterial properties requires reliable quantification of colonizing bacteria. there are many different methods to quantify biofilms on solid surfaces in vitro, employing different (bio-)chemical/microbiological reference parameters. it is therefore difficult to compare studies with different quantification techniques. here, we have evaluated commonly used microscopic, micro ... | 2016 | 27444546 |
| a cytolethal distending toxin variant from aggregatibacter actinomycetemcomitans with an aberrant cdtb that lacks the conserved catalytic histidine 160. | the periodontopathogen aggregatibacter actinomycetemcomitans synthesizes several virulence factors, including cytolethal distending toxin (cdt). the active cdt holoenzyme is an ab-type tripartite genotoxin that affects eukaryotic cells. subunits cdta and cdtc (b-components) allow binding and intracellular translocation of the active cdtb (a-component), which elicits nuclear dna damage. different strains of a. actinomycetemcomitans have diverse virulence genotypes, which results in varied pathoge ... | 2016 | 27414641 |
| antimicrobial effect of platelet-rich plasma and platelet-rich fibrin. | platelet concentrates have been extensively used in a variety of medical fields to promote soft- and hard-tissue regeneration. the significance behind their use lies in the abundance of growth factors (gfs) in platelets α-granules that promote wound healing. other than releasing a pool of gfs upon activation, platelets also have many features that indicate their role in the anti-infective host defense. | 2016 | 27411660 |
| interactions between lactobacillus rhamnosus gg and oral micro-organisms in an in vitro biofilm model. | probiotics have shown favourable properties in maintaining oral health. by interacting with oral microbial communities, these species could contribute to healthier microbial equilibrium. this study aimed to investigate in vitro the ability of probiotic lactobacillus rhamnosus gg (l.gg) to integrate in oral biofilm and affect its species composition. five oral strains, streptococcus mutans, streptococcus sanguinis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum and candida albican ... | 2016 | 27405227 |
| biofilm formation of periodontal pathogens on hydroxyapatite surfaces: implications for periodontium damage. | biofilm formation of periodontal pathogens on teeth surfaces promotes the progression of periodontal disease. hence, understanding the mechanisms of bacterial attachment to the dental surfaces may inform strategies for the maintenance of oral health. although hydroxyapatite (ha) is a major calcium phosphate component of teeth, effect of biofilm formation on ha surfaces remains poorly characterized. in this study, biofilm-forming abilities by the periodontal pathogens aggregatibacter actinomycete ... | 2016 | 27390886 |
| a commensal bacterium promotes virulence of an opportunistic pathogen via cross-respiration. | bacteria rarely inhabit infection sites alone, instead residing in diverse, multispecies communities. despite this fact, bacterial pathogenesis studies primarily focus on monoculture infections, overlooking how community interactions influence the course of disease. in this study, we used global mutant fitness profiling (transposon sequencing [tn-seq]) to determine the genetic requirements for the pathogenic bacterium aggregatibacter actinomycetemcomitans to cause disease when coinfecting with t ... | 2016 | 27353758 |
| fine-tuned production of hydrogen peroxide promotes biofilm formation of streptococcus parasanguinis by a pathogenic cohabitant aggregatibacter actinomycetemcomitans. | balanced bacterial biofilm communities help to maintain host health. disturbance of such balance can lead to bacterial dysbiosis and pathogenesis. however, complex and dynamic bacterial interactions within the biofilm communities are poorly understood. in this study, we used a dual-species biofilm consisting of the periodontal pathogen aggregatibacter actinomycetemcomitans, and a commensal streptococcus parasanguinis to investigate bacterial interactions since the two organisms have been found t ... | 2016 | 27348605 |
| is mechanical curettage with adjunct photodynamic therapy more effective in the treatment of peri-implantitis than mechanical curettage alone? | the aim of the present study was to review the pertinent literature on the effects of mechanical curettage (mc) with and without adjunct photodynamic therapy (pdt) for the management of peri-implantitis. | 2016 | 27344944 |
| periodontitis-associated pathogens p. gingivalis and a. actinomycetemcomitans activate human cd14(+) monocytes leading to enhanced th17/il-17 responses. | the th17/il-17 pathway is implicated in the pathogenesis of periodontitis (pd), however the mechanisms are not fully understood. we investigated the mechanism by which the periodontal pathogens porphyromonas gingivalis (pg) and aggregatibacter actinomycetemcomitans (aa) promote a th17/il-17 response in vitro, and studied il-17(+) cd4(+) t-cell frequencies in gingival tissue and peripheral blood from patients with pd versus periodontally healthy controls. addition of pg or aa to monocyte/cd4(+) t ... | 2016 | 27334899 |
| an in vitro comparison of antimicrobial effects of curcumin-based photodynamic therapy and chlorhexidine, on aggregatibacter actinomycetemcomitans. | considering the importance of prevention in periodontal diseases and the important role of aggregatibacter actinomycetemcomitans in induction and progression of these diseases, the aim of the present in vitro study was to compare the antimicrobial effects of chlorhexidine digluconate (chx), curcumin and light-emitting diode (led) laser, on this bacterium. | 2016 | 27330693 |
| periodontopathogens antibodies and major adverse events following an acute myocardial infarction: results from the french registry of acute st-elevation and non-st-elevation myocardial infarction (fast-mi). | periodontopathogens antibodies have been shown to be associated with primary myocardial events, but little is known regarding their impact on major adverse events after a prior acute myocardial infarction (ami). the present prospective study evaluates the association between antibody levels of 4 periodontopathogens and the risk of all-cause death or non-fatal myocardial infarction (mi) at 1 year in 975 patients admitted for acute st segment or non-st segment elevation mi in french registry of ac ... | 2016 | 27325868 |
| adjunct antimicrobial therapy and periodontal surgery to treat generalized aggressive periodontitis: a case report. | here we report a case of generalized aggressive periodontitis treated with periodontal therapy including adjunct antimicrobial therapy and periodontal surgery. the patient was a 22-year-old woman who presented with the chief complaint of gingival recession. baseline examination revealed generalized plaque deposition and gingival inflammation. thirty-nine percent of the sites had a probing depth (pd) of 4-6 mm and 2% a pd of ≥7 mm; 63% exhibited bleeding on probing (bop). radiographic examination ... | 2016 | 27320300 |
| a modified glycosaminoglycan, gm-0111, inhibits molecular signaling involved in periodontitis. | periodontitis is characterized by microbial infection, inflammation, tissue breakdown, and accelerated loss of alveolar bone matrix. treatment targeting these multiple stages of the disease provides ways to treat or prevent periodontitis. certain glycosaminoglycans (gags) block multiple inflammatory mediators as well as suppress bacterial growth, suggesting that these gags may be exploited as a therapeutic for periodontitis. | 2016 | 27308827 |
| a protein-repellent and antibacterial nanocomposite for class-v restorations to inhibit periodontitis-related pathogens. | the objectives of this study were to develop a bioactive dental composite and investigate the effects of 2-methacryloyloxyethyl phosphorylcholine (mpc) and dimethylaminohexadecyl methacrylate (dmahdm) in class v composite on mechanical properties, water sorption, protein adsorption, and inhibition of four species of periodontitis-related biofilms for the first time. the resin consisted of ethoxylated bisphenol a dimethacrylate (ebpadma) and pyromellitic glycerol dimethacrylate (pmgdm). dmahdm, m ... | 2016 | 27287170 |
| development of novel electrospun dual-drug fiber mats loaded with a combination of ampicillin and metronidazole. | our study was performed with the aim of preparing electrospun polylactide fibers with a combination of ampicillin (amp) and metronidazole (mnz) and investigating their drug release behavior and the antibacterial effect on aggregatibacter actinomycetemcomitans and other oral pathogens. | 2016 | 27262200 |
| [study of mutual dependence of periodontal and colonic microbiome in health and pathology using nsg-sequencing]. | by using ngs-sequencing libraries of dna from periodontal swabs with primers specific to v6 region of 16s rdna prevalence of bacterial genera and species in periodontal and colonic microbiota of patients with periodontitis of different severity and healthy donors was analyzed. hyper-colonization of the colon with akkermansia muciniphila was found to be the most important maker of negative predisposition to periodontitis (t=133,7 at р=10(-6)). this result is in a good agreement with communication ... | 2016 | 27239990 |
| influence of aae autotransporter protein on adhesion and biofilm formation by aggregatibacter actinomycetemcomitans. | the periodontopathogen aggregatibacter actinomycetemcomitans colonizes oral cavity by binding to and invading epithelial cells as well as by participating in biofilms formed on hard surfaces. aae, an autotransporter protein, is implicated in bacterial adhesion to epithelial cells. due to the multiple functions of bacterial autotransporter proteins, this study aimed to evaluate the role of aae in a. actinomycetemcomitans ability to adhere to both saliva-coated hydroxyapatite (sha) and biofilm. an ... | 2017 | 27224556 |
| sphingosine-1-phosphate receptor 2 regulates proinflammatory cytokine production and osteoclastogenesis. | sphingosine-1-phosphate receptor 2 (s1pr2) couples with the gi, gq, and g12/13 group of proteins, which modulate an array of cellular signaling pathways and affect immune responses to multiple stimuli. in this study, we demonstrated that knockdown of s1pr2 by a specific s1pr2 shrna lentiviral vector significantly inhibited il-1β, il-6, and tnf-α protein levels induced by oral pathogen aggregatibacter actinomycetemcomitans (a. actinomycetemcomitans) in murine bone marrow-derived monocytes and mac ... | 2016 | 27224249 |
| withaferin a inhibits inflammatory responses induced by fusobacterium nucleatum and aggregatibacter actinomycetemcomitans in macrophages. | periodontitis is a progressive chronic inflammatory disease and a major cause of tooth loss in humans. as a withanolides, withaferin a (wa) is known to exhibit strong anti‑inflammatory activity. the present study examined whether wa inhibited inflammatory responses in macrophages in response to two representative periodontal pathogens, fusobacterium nucleatum and aggregatibacter actinomycetemcomitans. murine bone marrow‑derived macrophages (bmdms) were used in this study and cytokine production ... | 2016 | 27220676 |
| analysis of subgingival plaque ability to stimulate toll-like receptor 2 and 4. | it has been shown that toll-like receptor (tlr) 2- and tlr4-stimulating abilities of supragingival plaque (spp) are associated with periodontal conditions. it is hypothesized that spp might affect the periodontium through its influence on subgingival plaque (sbp). this study investigates relationships between tlr2- and tlr4-stimulating abilities of sbp and periodontal conditions. | 2016 | 27212110 |
| [use of the real-time pcr for study of the periodontal microbiome in patients with combined pathology of gastroduodenal zone and chronic periodontitis]. | the total of 54 patients with chronic periodontitis of different severity was tested using real-time pcr (dentoflor kit). the group included 38 patients with chronic gastritis. for the first time, a higher prevalence of treponema denticola in periodontium of males in comparison with females was demonstrated. the patients with chronic gastritis had more human genome dna at their periodontium than healthy individuals. non-parametric statistical analysis demonstrated high association of periodontiu ... | 2016 | 27183718 |
| bactericidal effect of erbium-doped yttrium aluminum garnet laser and photodynamic therapy on aggregatibacter actinomycetemcomitans biofilm on implant surface. | peri-implantitis is a common complication of dental implants. the first step of treatment is elimination of bacterial biofilm and disinfection of the implant surface. this study sought to compare the effects of an erbium-doped yttrium aluminum garnet (er:yag) laser, photodynamic therapy using an indocyanin green-based photosensitizer (icg-based ps) and diode laser, toluidine blue o (tbo) photosensitizer and light-emitting diode (led) light source, and 2% chlorhexidine (chx) on biofilm of aggrega ... | 2017 | 27183085 |
| [microbiological approach to a possible infective endocarditis case caused by aggregatibacter actinomycetemcomitans]. | aggregatibacter (actinobacillus) actinomycetemcomitans, a small, gram-negative coccobacillus that grows slow and fastidious, is generally colonized in the oral cavity. it is a rarely seen bacterium because of the difficulty of isolation but it can be a causative agent for dental infections and infective endocarditis (ie) particularly in the persons having prosthetic heart valves. in this report, a possible ie case caused by a.actinomycetemcomitans in a patient with aortic valve replacement has b ... | 2016 | 27175505 |
| apoptotic effects of the b subunit of bacterial cytolethal distending toxin on the a549 lung cancer cell line. | cytolethal distending toxin (cdt) is a secreted tripartite genotoxin produced by many pathogenic gram-negative bacteria. it is composed of three subunits, cdta, cdtb and cdtc, and cdtb-associated deoxyribonuclease (dnase) activity is essential for the cdt toxicity. in the present study, to design a novel potentially antitumor drug against lung cancer, the possible mechanisms of cdtb anticancer properties were explored in the a549 human lung adenocarcinoma cell line. a recombinant plasmid pcdna3. ... | 2016 | 27165242 |
| antimicrobial efficacy of tulsi leaf (ocimum sanctum) extract on periodontal pathogens: an in vitro study. | periodontitis is an infection of the periodontal complex with severe forms of disease associated with specific bacteria colonizing the subgingival area. widespread use of drugs has resulted in the emergence of side effects, uncommon infections, and resistance. plant medicine like tulsi has been used in many clinical conditions, and it appears to be a suitable alternative to manage conditions affecting the oral cavity. hence, the objective was to assess the in vitro antimicrobial activity of tuls ... | 2017 | 27143825 |
| occurrence of aggregatibacter actinomycetemcomitans in indian chronic periodontitis patients and periodontally healthy adults. | aggregatibacter actinomycetemcomitans (aa), an important primary periodontal pathogen, is known for its strong virulence characteristics that cause periodontal disease. we investigated aa occurrence in indian individuals using culture and 16 s rdna polymerase chain reaction (pcr). | 2017 | 27143824 |
| detection of five potentially periodontal pathogenic bacteria in peri-implant disease: a comparison of pcr and real-time pcr. | the aim of this study was to compare the microbial analysis methods of polymerase chain reaction (pcr) and real-time pcr (rt-pcr) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using pcr (dna-strip technology) and rt-pcr (fluorescent dye sybr green-system): aggregatibacter actinomycetemcomitans (aa ... | 2016 | 27142589 |
| comparative evaluation of antimicrobial activity of pomegranate-containing mouthwash against oral-biofilm forming organisms: an invitro microbial study. | pomegranate is considered "a pharmacy unto itself". hydrolysable tannins called punicalagins which have free scavenging properties are the most abundant polyphenols found in pomegranate-containing mouthwash. | 2016 | 27135005 |
| anti-microbial activity of tulsi {ocimum sanctum (linn.)} extract on a periodontal pathogen in human dental plaque: an invitro study. | tulsi is a popular healing herb in ayurvedic medicine. it is widely used in the treatment of several systemic diseases because of its anti-microbial property. however, studies documenting the effect of tulsi on oral disease causing organisms are rare. hence, an attempt was made to determine the effect of tulsi on a periodontal microorganism in human dental plaque. | 2016 | 27135002 |
| hacek endocarditis: a review. | the hacek group, referring to haemophilus spp., aggregatibacter actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae, is a rare cause of infective endocarditis (ie). it causes the majority of gram-negative endocarditis cases and has an excellent prognosis and simple management if properly identified. however, delay in diagnosis and associated complications can render the infection fatal. | 2016 | 27124204 |
| aggregatibacter actinomycetemcomitans outer membrane protein 29 (omp29) induces tgf-β-regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrinβ1/fak cascade. | gingival junctional epithelial cell apoptosis caused by periodontopathic bacteria exacerbates periodontitis. this pathological apoptosis is involved in the activation of transforming growth factor β (tgf-β). however, the molecular mechanisms by which microbes induce the activation of tgf-β remain unclear. we previously reported that aggregatibacter actinomycetemcomitans (aa) activated tgf-β receptor (tgf-βr)/smad2 signalling to induce epithelial cell apoptosis, even though aa cannot bind to tgf- ... | 2016 | 27121139 |
| alteration of homeostasis in pre-osteoclasts induced by aggregatibacter actinomycetemcomitans cdt. | the dysbiotic microbiota associated with aggressive periodontitis includes aggregatibacter actinomycetemcomitans, the only oral species known to produce a cytolethal distending toxin (aacdt). give that cdt alters the cytokine profile in monocytic cells, we aimed to test the hypothesis that cdt plays a role in bone homeostasis by affecting the differentiation of precursor cells into osteoclasts. recombinant aacdt was added to murine bone marrow monocytes (bmmc) in the presence or absence of rankl ... | 2016 | 27064424 |
| efficacy of photodynamic therapy and lasers as an adjunct to scaling and root planing in the treatment of aggressive periodontitis - a clinical and microbiologic short term study. | aggressive periodontitis comprises a group of rare, severe, rapidly progressive form of periodontitis. conventional treatment includes mechanical debridement augmented with adjunctive antimicrobial therapy. development of antibiotic resistance has led to use of lasers. photodynamic therapy (pdt) is a novel non-invasive therapeutic approach with increased site and pathogen specificity. this study compares pdt and lasers as an adjunct to conventional scaling in the treatment of patients with aggre ... | 2016 | 27042576 |
| inhibition of bacterial toxin activity by the nuclear stain, draq5™. | the repeats-in-toxin family of toxins includes proteins produced by gram negative bacteria such as escherichia coli (α-hemolysin), bordetella pertussis (adenylate cyclase toxin), and aggregatibacter actinomycetemcomitans (ltxa), which contribute to the pathogenesis of these organisms by killing host cells. in the case of ltxa produced by a. actinomycetemcomitans, white blood cells are targeted, allowing the bacteria to avoid clearance by the host immune system. in its association with target cel ... | 2016 | 27039399 |
| antibacterial effect of silver (i) carbohydrate complexes on oral pathogenic key species in vitro. | it was the aim of this study to evaluate the antibacterial impact of two silver(i) carbohydrate complexes with tripodal thioglycosides, namely tris[2-(β-d-thio-glucopyranosyl)ethyl]-amine-silver(i)-nitrate (3) and tris[2-(α-d-thio-manno-pyranosyl)ethyl]-amine-silver(i)-nitrate (4), on five oral pathogenic bacterial strains. furthermore, cytocompatibility was tested using human gingival fibroblasts (hgf). | 2016 | 27009305 |
| complete genome sequence of aggregatibacter actinomycetemcomitans serotype g strain num4039 (jcm 30399). | aggregatibacter actinomycetemcomitans is considered to be a major etiological agent of aggressive periodontitis and includes serotype a to g strains. we herein report the first complete genome sequence of a. actinomycetemcomitans serotype g strain num4039. the genome is 2,382,853 bp in length with a g+c content of 44.34%. | 2016 | 26988057 |
| profound effects of aggregatibacter actinomycetemcomitans leukotoxin mutation on adherence properties are clarified in in vitro experiments. | leukotoxin (ltx) is a prominent virulence factor produced by aggregatibacter actinomycetemcomitans, an oral microorganism highly associated with aggressive periodontitis. ltx compromises host responsiveness by altering the viability of neutrophils, lymphocytes, and macrophages. previously, we developed a rhesus (rh) monkey colonization model designed to determine the effect of virulence gene mutations on colonization of a. actinomycetemcomitans. unexpectedly, an a. actinomycetemcomitans leukotox ... | 2016 | 26977924 |
| influence of a triclosan toothpaste on periodontopathic bacteria and periodontitis progression in cardiovascular patients: a randomized controlled trial. | triclosan/copolymer toothpaste is effective in controlling plaque and gingivitis and in slowing the progression of periodontitis. this study describes its influence on microbiological and clinical outcomes, over a 5-year period, in patients with established cardiovascular disease (cvd). | 2017 | 26932733 |
| a bacterial-biofilm-induced oral osteolytic infection can be successfully treated by immuno-targeting an extracellular nucleoid-associated protein. | periodontal disease exemplifies a chronic and recurrent infection with a necessary biofilm component. mucosal inflammation is a hallmark response of the host seen in chronic diseases, such as colitis, gingivitis, and periodontitis (and the related disorder peri-implantitis). we have taken advantage of our recently developed rat model of human peri-implantitis that recapitulates osteolysis, the requirement of biofilm formation, and the perpetuation of the bona fide disease state, to test a new th ... | 2017 | 26931773 |
| early colonization of the oral cavity in 6- and 12-month-old infants by cariogenic and periodontal pathogens: a case-control study. | the colonization of the oral cavity by cariogenic and periodontal pathogens occurs earlier than previously thought. this study aimed to identify the presence and quantity of representative cariogenic and periodontal pathogens in the oral cavities of 6- and 12-month olds and to evaluate the influence of c-section delivery on early streptococcus mutans (sm) colonization of the oral cavity. the research cohort was composed of 59 infants (35 infants were delivered vaginally and 24 via c-section) and ... | 2016 | 26914065 |
| quantitative pcr studies of aggregatibacter actinomycetemcomitans and treponema denticola/tanerella forsythensis complex as etiological factors of chronic periodontitis. | real-time quantitative pcr (dentofl or kit) was used to detect dna of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. a bimodal distribution of patients by periodontium colonization with a. actinomycetemcomitans, p. gingivalis, p. intermedia, t. forsythensis, and t. denticola was demonstrated. a new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of ... | 2016 | 26899837 |
| macrophage polarization in response to oral commensals and pathogens. | macrophages have been identified in the periodontium. data have phenotypically described these cells, demonstrated changes with progressing periodontal disease, and identified their ability to function in antigen-presentation critical for adaptive immune responses to individual oral bacterium. recent evidence has emphasized an important role for the plasticity of macrophage phenotypes, not only in the resulting function of these cells in various tissues, but also clear differences in the stimula ... | 2016 | 26884502 |
| aggregatibacter actinomycetemcomitans-induced aim2 inflammasome activation is suppressed by xylitol in differentiated thp-1 macrophages. | aggressive periodontitis is characterized by rapid destruction of periodontal tissue caused by aggregatibacter actinomycetemcomitans. interleukin (il)-1β is a proinflammatory cytokine, and its production is tightly regulated by inflammasome activation. xylitol, an anticaries agent, is anti-inflammatory, but its effect on inflammasome activation has not been researched. this study investigates the effect of xylitol on inflammasome activation induced by a. actinomycetemcomitans. | 2016 | 26876350 |
| antimicrobial effects of commensal oral species are regulated by environmental factors. | the objectives of this study are to identify oral commensal species which can inhibit the growth of the main periodontopathogens, to determine the antimicrobial substances involved in these inhibitory activities and to evaluate the influence of environmental factors on the magnitude of these inhibitions. | 2016 | 26875613 |
| inverse association of plasma igg antibody to aggregatibacter actinomycetemcomitans and high c-reactive protein levels in patients with metabolic syndrome and periodontitis. | the association between clinically diagnosed periodontitis, a common chronic oral infection, and metabolic syndrome has been previously reported. the aim of this study was to investigate the association of plasma igg levels against aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia, c-reactive protein, and periodontal status with metabolic syndrome. plasma igg levels and c-reactive protein were measured by enzyme-linked immunosorbent assay, and salivary le ... | 2016 | 26871443 |
| microbiological distribution of six periodontal pathogens between untreated italian and dutch periodontal patients. | to compare prevalence and microbial load of six periodontal pathogens between italian and dutch patients affected by chronic periodontitis, using oligonucleotide probe technology. | 2016 | 26870849 |
| diabetic lactoferrin deficient mice demonstrates greater susceptibility to experimental periodontal disease. | the objective of this study is to detrmine whether alloxan-induced diabetic lactoferrin knockout (lfko(-/-)) mice are more susceptible to periodontal disease caused by aggregatibacter actinomycetemcomitans compared to the diabetic wild-type (wt) mice. diabetes was induced in mice by a single dose of alloxan (60 mg/kg) injected intravenously. mice were categorized as diabetic when blood glucose levels >250 mg/dl were measured on the 7(th) day after the injection. periodontal disease was experimen ... | 2017 | 26855973 |
| bioactive glass combined with bisphosphonates provides protection against biofilms formed by the periodontal pathogen aggregatibacter actinomycetemcomitans. | biofilms play a pivotal role in the progression of periodontitis and they can be treated with antiseptics (i.e. chlorhexidine) or antibiotics, but these therapeutic alternatives are unable of ameliorating periodontal alveolar bone loss, which has been, on the other hand, successfully treated with bone-preserving agents. the improved bone formation achieved in animal models by the combination of two such agents: bioactive glass (bag) and bisphosphonates has attracted the interest for further expl ... | 2016 | 26854428 |
| actinobacillus actinomycetemcomitans keratitis after glaucoma infiltration surgery: a clinical report and literature review. | actinobacillus actinomycetemcomitans infection is a rare and easily misdiagnosed ocular disease. in this article, the authors report a chronic, purulent, and difficult-to-treat case of a actinomycetemcomitans keratitis following a glaucoma infiltration surgery.a 56-year-old man with a long-standing history of open-angle glaucoma in both eyes presented with a 12-week history of ocular pain, redness, and blurred vision in his right eye. he underwent a glaucoma infiltration surgery in his right eye ... | 2016 | 26817919 |
| differential expression of cc chemokines (ccls) and receptors (ccrs) by human t lymphocytes in response to different aggregatibacter actinomycetemcomitans serotypes. | in aggregatibacter actinomycetemcomitans, different serotypes have been described based on lps antigenicity. recently, our research group has reported a differential immunogenicity when t lymphocytes were stimulated with these different serotypes. in particular, it was demonstrated that the serotype b of a. actinomycetemcomitans has a stronger capacity to trigger th1- and th17-type cytokine production. objective this study aimed to quantify the expression of different cc chemokines (ccls) and re ... | 2016 | 26814460 |
| oral and endotracheal tubes colonization by periodontal bacteria: a case-control icu study. | periodontal infection is a possible risk factor for respiratory disorders; however, no studies have assessed the colonization of periodontal pathogens in endotracheal tubes (et). this case-control study analyzed whether periodontal pathogens are able to colonize et of dentate and edentulous patients in intensive care units (icu) and whether oral and et periodontal pathogen profiles have any correlation between these patients. we selected 18 dentate and 18 edentulous patients from 78 eligible icu ... | 2016 | 26810057 |
| [role of platelet-activating factor receptor in adhesion and invasion of aggregatibacter actinomycetemcomitans in human umbilical vein endothelial cells]. | to explore the role of platelet-activating factor receptor (pafr) in adhesion and invasion of phospho- rylcholine (pc)-positive aggregatibacter actinomycetemcomitans in cultured human umbilical vein endothelial cells (huvec). | 2016 | 26806742 |
| salivary pathogen and serum antibody to assess the progression of chronic periodontitis: a 24-mo prospective multicenter cohort study. | a diagnosis of periodontitis progression is presently limited to clinical parameters such as attachment loss and radiographic imaging. the aim of this multicenter study was to monitor disease progression in patients with chronic periodontitis during a 24-mo follow-up program and to evaluate the amount of bacteria in saliva and corresponding igg titers in serum for determining the diagnostic usefulness of each in indicating disease progression and stability. | 2016 | 26791469 |
| characterization of a natural mouse monoclonal antibody recognizing epitopes shared by oxidized low-density lipoprotein and chaperonin 60 of aggregatibacter actinomycetemcomitans. | natural antibodies are predominantly antibodies of the igm isotype present in the circulation of all vertebrates that have not been previously exposed to exogenous antigens. they are often directed against highly conserved epitopes and bind to ligands of varying chemical composition with low affinity. in this study we cloned and characterized a natural mouse monoclonal igm antibody selected by binding to malondialdehyde acetaldehyde epitopes on low-density lipoprotein (ldl). interestingly, the i ... | 2016 | 26786003 |
| effect of photodynamic therapy adjunct to scaling and root planing in periodontitis patients: a randomized clinical trial. | a randomized split-mouth controlled clinical trial was conducted to evaluate the efficacy of photodynamic therapy (pdt) in reducing aggregatibacter actinomycetemcomitans (aa) in periodontitis patients. | 2016 | 26780271 |
| strontium-substituted bioactive glasses in vitro osteogenic and antibacterial effects. | bioactive glass forms a bone mineral apatite interface and can be engineered to promote optimal bone regeneration. strontium (sr(2+)) stimulates osteoblast and inhibits osteoclast activities in vitro, and is used clinically as a treatment for osteoporosis. dental bone defect repair requires rapid bone formation for early osseointegration but, can be subject to infection. the aim of this study was to investigate the osteogenic and antibacterial effects of strontium-substituted bioactive glasses i ... | 2016 | 26777094 |
| effect of low-level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5-fluorouracil chemotherapy. | this study aimed to assess the effect of multiple sessions of a low-level laser therapy (lllt) adjuvant to scaling and root planing (srp) on the treatment of experimental periodontitis (ep) in rats treated with 5-fluorouracil (5-fu). | 2016 | 26750739 |
| in vitro behavior of poly-lactic-co-glycolic acid microspheres containing minocycline, metronidazole, and ciprofloxacin. | in the present study, we aimed to fabricate poly-lactic-co-glycolic acid (plga) microspheres containing a mixture of three antibiotics-minocycline, metronidazole, and ciprofloxacin (mmc)-to assess their efficacy and properties. | 2016 | 26748575 |
| systematic review of the in vitro effects of statins on oral and perioral microorganisms. | statins are medications administered orally and are widely used for lowering the blood cholesterol level. the aim of this study was to investigate the effects of orally administered statins on microorganisms infecting oral and perioral tissues. we performed a systematic review of published studies of the in vitro antimicrobial effects of statins on bacteria, viruses, and fungi, and searched pubmed, web of science, cochrane central, and google scholar. studies show that most statins exhibit antim ... | 2016 | 26718458 |
| evaluation of antimicrobial action of carie care™ and papacarie duo™ on aggregatibacter actinomycetemcomitans a major periodontal pathogen using polymerase chain reaction. | in the present scenario, we are made available with chemomechanical caries removal system containing a natural proteolytic enzyme for the ease in the excavation of infected dentine. the additive action for these agents is providing antimicrobial and anti-inflammatory properties. | 2017 | 26681861 |
| scaling and root planning, and locally delivered minocycline reduces the load of prevotella intermedia in an interdependent pattern, correlating with symptomatic improvements of chronic periodontitis: a short-term randomized clinical trial. | to evaluate the respective or combinatory efficacy of locally delivered 2% minocycline (mo), and scaling and root planning (srp) by assessing both clinical parameters and the loads of four main periodontal pathogens in treating chronic periodontitis (cp). | 2015 | 26676022 |
| in vitro antibacterial activity of ethanolic extract of morus alba leaf against periodontal pathogens. | antibiotic resistance is a major problem with inadvertent usage. thus, there is a need to search for new antimicrobial agents of herbal origin to combat antibiotic resistance. one such plant is morus alba which has a long history of medicinal use in traditional chinese medicine. | 2015 | 26672427 |
| evaluation of the efficacy of a probiotic drink containing lactobacillus casei on the levels of periodontopathic bacteria in periodontitis: a clinico-microbiologic study. | this study was designed to evaluate whether the oral administration of lactobacilli could change the bacterial population in subgingival plaque. | 2015 | 26672414 |
| maresin 1 biosynthesis and proresolving anti-infective functions with human-localized aggressive periodontitis leukocytes. | localized aggressive periodontitis (lap) is a distinct form of early-onset periodontitis linked to periodontal infection with uncontrolled inflammation and leukocyte-mediated tissue destruction. the resolution of inflammation is an active process orchestrated by specialized proresolving lipid mediators (spms). since the level of the maresin pathway marker 14-hydroxy-docosahexaenoic acid (14-hdha) was lower in activated peripheral blood from lap patients, we investigated the maresin 1 (mar1) bios ... | 2015 | 26667839 |
| short-term microbiological effects of photodynamic therapy in non-surgical periodontal treatment of residual pockets: a split-mouth rct. | photodynamic therapy (pdt) has been used as a therapeutic alternative to treat periodontitis, especially in challenging sites that require additional periodontal therapy such as residual pockets. the aim of this split-mouth randomized trial was to evaluate the microbiological and clinical effects of pdt on non-surgical treatment of unresponsive pockets. | 2016 | 26660720 |
| the jp2 genotype of aggregatibacter actinomycetemcomitans and marginal periodontitis in the mixed dentition. | to perform a cross-sectional study on the carrier frequency of jp2 and non-jp2 genotypes of a. actinomycetemcomitans in moroccan school children and relate the presence of these genotypes to the periodontal status in the mixed dentition. | 2016 | 26659719 |
| effect of periodontitis on adiponectin, c-reactive protein, and immunoglobulin g against porphyromonas gingivalis in thai people with overweight or obese status. | obesity and periodontitis are associated with an inflammatory background. inflammatory mediators involved may have reciprocal effects on one another. in this study, the levels of inflammatory mediators implicated in overweight or obese status and periodontitis are simultaneously evaluated. | 2016 | 26654348 |
| periodontitis prevalence and serum antibody reactivity to periodontal bacteria in primary sjögren's syndrome: a pilot study. | the aims of this study were as follows: (i) to assess the prevalence of periodontitis among patients with primary sjögren's syndrome (pss) and comparator groups of patients with rheumatoid arthritis (ra) and osteoarthritis (oa). (ii) to perform a pilot study to compare serum antibody responses to 10 oral/periodontal bacteria in these patient groups and a historical comparator group of patients with periodontitis. | 2016 | 26646777 |
| neutrophil proteolytic activation cascades: a possible mechanistic link between chronic periodontitis and coronary heart disease. | cardiovascular diseases are chronic inflammatory diseases that affect a large segment of society. coronary heart disease (chd), the most common cardiovascular disease, progresses over several years and affects millions of people worldwide. chronic infections may contribute to the systemic inflammation and enhance the risk for chd. periodontitis is one of the most common chronic infections that affects up to 50% of the adult population. under inflammatory conditions the activation of endogenous d ... | 2016 | 26608308 |
| changes in salivary periodontal pathogens after orthodontic treatment: an in vivo prospective study. | to analyze the initial changes in salivary levels of periodontal pathogens after orthodontic treatment with fixed appliances. | 2016 | 26606331 |
| comparative evaluation of the efficacy of curcumin gel with and without photo activation as an adjunct to scaling and root planing in the treatment of chronic periodontitis: a split mouth clinical and microbiological study. | harnessing mother nature's bountiful remedies for rejuvenation has been in vogue since time immemorial. turmeric contains the polyphenol curcumin in its rhizome. it produces reactive oxygen species (ros) with visible light irradiation as photodynamic therapy (pdt) - which validates its use in the treatment of periodontitis. this study compares curcumin and curcumin pdt as an adjunct to conventional scaling and root planing (srp) with srp alone in the treatment of patients with chronic periodonti ... | 2015 | 26604595 |