| atypical microorganisms in expressed prostatic secretion from patients with chronic prostatitis/chronic pelvic pain syndrome: microbiological results from a case-control study. | to investigate the microorganisms in expressed prostatic secretions (eps) of both chronic prostatitis patients and normal young male adults and to determine which microorganisms are associated with the degree of intraprostatic inflammation. | 2013 | 23970289 |
| association of chlamydia trachomatis infection and herpes simplex virus type 2 serostatus with genital human papillomavirus infection in men: the hpv in men study. | studies in women indicate that some sexually transmitted infections promote human papillomavirus (hpv) persistence and carcinogenesis. little is known about this association in men; therefore, we assessed whether chlamydia trachomatis (ct) infection and herpes simplex virus type 2 (hsv-2) serostatus are associated with genital hpv prevalence, an early event in hpv-related pathogenesis. | 2013 | 23680908 |
| trends and associations of trichomonas vaginalis infection in men and women with genital discharge syndromes in johannesburg, south africa. | to better understand the epidemiology of trichomonas vaginalis infection, we investigated the association between t vaginalis and demographic, clinical, microbiological and behavioural characteristics of patients presenting with genital discharges to a primary healthcare clinic in johannesburg, south africa. | 2013 | 23605850 |
| a retrospective study into the prevalence of herpes simplex virus 1&2 in female patients tested for chlamydia trachomatis and/or neisseria gonorrhoeae using vaginal swabs. | the purpose of this study was to investigate the prevalence of herpes simplex virus (hsv) 1&2 in women screened for chlamydia trachomatis and/or neisseria gonorrhoeae (ct/gc) infections. a total of 800 vaginal swabs were tested in a dual centre investigation, to evaluate the prevalence of hsv-1&2 in this population. the average age of the population was 29.8 ± 9.2 years, ranging between 14 and 66 years with a median of 28 years. the highest prevalence of hsv-1 or hsv-2 was observed in the younge ... | 2013 | 23320468 |
| herpes simplex virus type 2 risks in female sex workers in the china-vietnam border county of hekou. | to survey the prevalence and risk factors of hsv-2 among chinese and vietnamese female sex workers (fsw) in the border county of hekou, yunnan province, china. | 2012 | 23228841 |
| prevalence and associations of genital ulcer and urethral pathogens in men presenting with genital ulcer syndrome to primary health care clinics in south africa. | this study aimed to determine the prevalence of genital ulcer and urethral pathogens, as well as their association with clinical features, in men with genital ulcer disease (gud) enrolled in a clinical trial. | 2012 | 23064538 |
| [epidemiology and risk factors of the cervical squamous cell carcinoma]. | neoplasm of the cervix, especially squamous cell cancer, is one of the most common malignancy of female genital organs. it etiology is complex; however, human papilloma virus (mostly hpv type 18, 16 and 45) infection seems to be the most important one. other risk factors include: early sexual initiation, multiple pregnancies and labors, concomitant infections (chlamydia trachomatis, neisseria gonorrhea, hsv2 - herpes simplex virus) of the genital tract, aids, immunosuppressive therapy, smoking a ... | 2012 | 23009008 |
| looking for an asymptomatic infection: usefulness of screening for chlamydia trachomatis and genital herpes. | chlamydia trachomatis (ct) and herpes simplex virus type 2 (hsv-2) genital infections are among the more frequent sexually transmissible infections with different prevalence by age, populations and geographical areas. both are widespread, have an asymptomatic latent stage detectable only by laboratory and can have serious consequences such as tubal infertility and neonatal herpes. the direct isolation of ct in cervical or urethral discharge through nucleic acid amplification test (naats) allows ... | 2012 | 23007249 |
| etiologic pattern of genital ulcers in lusaka, zambia: has chancroid been eliminated? | genital ulcers are a public health problem in developing countries. the world health organization recommends the use of syndromic guidelines for sexually transmitted infection treatment in resource-constrained countries. monitoring local etiologies provides information that may aid policy for sexually transmitted infection treatment. we investigated the etiology of genital ulcer disease among outpatients in lusaka, zambia. | 2012 | 23001266 |
| sexually transmitted infections and sexual behaviour among youth clients of hotel-based female sex workers in dhaka, bangladesh. | a cross-sectional study was conducted among youth clients of hotel-based female sex workers (ychbfsws) in nine randomly selected hotels in bangladesh to examine sexual-risk behaviour, condom use and determinants of condom use in last sex, knowledge of hiv, sexually transmitted infection (sti) prevalence and sti care-seeking behaviour. a prestructured questionnaire was used to collect sociodemographic, behavioural, clinical information; urine specimens (before sex) and blood were collected for di ... | 2012 | 22930291 |
| sperm-mediated host-derived dna transfer as a new mechanism for immune system evasion of sexually transmitted genital tract pathogens. | over one century of extensive efforts directed towards investigating the immune response and the immuno-protection associated with sexually transmitted infections have failed to produce any effective vaccines against most of the major pathogens, among them neisseria gonorrhea, herpes simplex virus type 2, and chlamydia trachomatis. attempts to design and develop protective vaccines against them have also yielded disappointing results. it has long been felt that there might be another yet undisco ... | 2012 | 22795612 |
| hiv and sexually transmitted infections at the borderlands: situational analysis of sexual health in the brazilian amazon. | the borderlands are considered areas of increased vulnerability to hiv and sexually transmitted infections (sti). the study aimed to determine the sti/hiv prevalence and risk factors in the triple-border area of the brazilian amazon. | 2012 | 22317841 |
| a role for h/aca and c/d small nucleolar rnas in viral replication. | we have employed gene-trap insertional mutagenesis to identify candidate genes whose disruption confer phenotypic resistance to lytic infection, in independent studies using 12 distinct viruses and several different cell lines. analysis of >2,000 virus-resistant clones revealed >1,000 candidate host genes, approximately 20 % of which were disrupted in clones surviving separate infections with 2-6 viruses. interestingly, there were 83 instances in which the insertional mutagenesis vector disrupte ... | 2014 | 24477674 |
| synthesis and antiviral activity of 6-deoxycyclopropavir, a new prodrug of cyclopropavir. | synthesis of 6-deoxycyclopropavir (10), a prodrug of cyclopropavir (1) and its in vitro and in vivo antiviral activity is described. 2-amino-6-chloropurine methylenecyclopropane 13 was transformed to its 6-iodo derivative 14 which was reduced to prodrug 10. it is converted to cyclopropavir (1) by the action of xanthine oxidase and this reaction can also occur in vivo. compound 10 lacked significant in vitro activity against human cytomegalovirus (hcmv), human herpes virus 1 and 2 (hsv-1 and hsv- ... | 2012 | 22417649 |
| the infectious etiology of alzheimer's disease. | inflammation is a part of the first line of defense of the body against invasive pathogens, and plays a crucial role in tissue regeneration and repair. a proper inflammatory response ensures the suitable resolution of inflammation and elimination of harmful stimuli, but when the inflammatory reactions are inappropriate it can lead to damage of the surrounding normal cells. the relationship between infections and alzheimer's disease (ad) etiology, especially late-onset ad (load) has been continuo ... | 2017 | 28294067 |
| investigation on the association between thyroid tumorigeneses and herpesviruses. | herpesviruses have been associated with various human malignancies and with thyroid autoimmunity. aiming to investigate the presence of these viruses in thyroid nodules, we analyzed serum and thyroid tissue from 183 patients (83 benign and 100 malignant thyroid nodules). we also obtained 104 normal thyroid tissues extracted from the contralateral lobe of these patients. we used elisa to screen the serology of all patients and a real-time quantitative pcr to analyze thyroid tissue viral load in a ... | 2017 | 28276007 |
| effect of mycoplasma hominis and cytomegalovirus infection on pregnancy outcome: a prospective study of 200 mongolian women and their newborns. | in mongolia, diagnostic tests for the detection of the sexually transmitted mycoplasmas, ureaplasmas, herpes simplex virus (hsv), and cytomegalovirus (cmv) are currently not routinely used in clinical settings and the frequency of these stis are enigmatic. the prevalence of these sti pathogens were prospectively evaluated among 200 mongolian pregnant women and their newborns and correlated with pregnancy outcome. taqman pcrs were used to detect bacterial and viral sti pathogens in pre-birth vagi ... | 2017 | 28257513 |
| maternal immunoreactivity to herpes simplex virus 2 and risk of autism spectrum disorder in male offspring. | maternal infections during pregnancy are associated with risk of neurodevelopmental disorders, including autism spectrum disorders (asds). proposed pathogenetic mechanisms include fetal infection, placental inflammation, and maternal cytokines or antibodies that cross the placenta. the autism birth cohort comprises mothers, fathers, and offspring recruited in norway in 1999 to 2008. through questionnaire screening, referrals, and linkages to a national patient registry, 442 mothers of children w ... | 2017 | 28251181 |
| immunohistochemistry is rarely justified for the diagnosis of viral infections. | to determine the utility of immunohistochemistry (ihc) for the diagnosis of viral infections in surgical pathology specimens lacking characteristic viral cytopathic effects. | 2016 | 28007703 |
| oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy treatment for head and neck squamous cell carcinoma. | opportunistic infections may affect the oral mucosa of patients undergoing radio/chemotherapy through exacerbation of oral mucositis. the aim of this study is to evaluate the oral shedding of all eight human herpesviruses and its possible association with oral mucositis. | 2016 | 27966015 |
| effects of cumulative herpesviridae and toxoplasma gondii infections on cognitive function in healthy, bipolar, and schizophrenia subjects. | schizophrenia and bipolar disorder are associated with cognitive impairment leading to social disruption. while previous studies have focused on the effect of individual infectious exposure, namely, herpesviridae viruses or toxoplasma gondii (t gondii), on cognitive functioning, the objective of the present study was to examine the effect of multiple infections on cognitive functioning in patients with schizophrenia and bipolar disorder and in healthy controls. | 2017 | 27929612 |
| infection and inflammation in schizophrenia and bipolar disorder. | the present study investigated the relationship between exposure to infectious agents and inflammation markers in individuals with schizophrenia (sz), bipolar disorder (bp), and controls without a psychiatric disorder. we measured plasma levels of antibodies and innate immune markers and correlated them with clinical symptoms and cognitive function. in both sz and bp, we found an increase in soluble cd14, and in bp an increase in c-reactive protein, igm class antibodies against cytomegalovirus ( ... | 2017 | 27856235 |
| common infections with polyomaviruses and herpesviruses and neuropsychological development at 4 years of age, the rhea birth cohort in crete, greece. | viral infections of the central nervous system may have detrimental effects for the developing brain, but the effects of less virulent common infections are unclear. we aim to investigate the impact of common viral infections of early childhood on neuropsychological performance of children at age four. | 2016 | 27334233 |
| shedding of hsv-1, hsv-2, cmv, and ebv in the saliva of hematopoietic stem cell transplant recipients at fundación homi - hospital de la misericordia, bogotá, d.c. | hematopoietic stem cell transplantation in pediatric patients is an alternative treatment for different diseases. the conditioning regimen for transplant predisposes recipients to the development of infections. viral infections by herpes simplex virus 1 (hsv-1), herpes simplex virus 2 (hsv-2), human cytomegalovirus (cmv), and epstein-barr virus (ebv), are the most common, and the leading cause of morbidity and mortality among these patients. these viruses lie dormant in various cell types and th ... | 2016 | 27622810 |
| the role of human papilloma virus and herpes viruses in the etiology of nasal polyposis. | the aim of this study was to investigate the etiological role of human papilloma virus (hpv), herpes simplex virus (hsv), varicella-zoster virus (vzv), epstein-barr virus (ebv), cytomegalovirus (cmv), and human herpes virus-6 (hhv-6) and -7 (hhv-7) in the occurrence of nasal polyposis. | 2016 | 27511490 |
| assessment of igg antibodies against hsv1, hsv2, cmv and ebv in patients with pemphigus vulgaris versus healthy people. | regarding the implication of viruses particularly herpes in pemphigus vulgaris, we sought to assess and compare the level of immunoglobulin g (igg) antibodies against herpes simplex virus types 1 and 2 (hsv1 and hsv2), cytomegalovirus (cmv) and epstein-barr virus (ebv) in patients with pemphigus vulgaris and healthy people. | 2015 | 27507994 |
| virus reactivations after autologous hematopoietic stem cell transplantation detected by multiplex pcr assay. | several studies have indicated that viral reactivations following allogeneic hematopoietic stem cell transplantation (allo-hsct) are frequent, but viral reactivations after autologous hsct (auto-hsct) have not been investigated in detail. we performed multiplex polymerase chain reaction (pcr) assay to examine multiple viral reactivations simultaneously in 24 patients undergoing auto-hsct between september 2010 and december 2012. weekly whole blood samples were collected from pre- to 42 days post ... | 2017 | 27364410 |
| immunological signaling during herpes simplex virus-2 and cytomegalovirus vaginal shedding after initiation of antiretroviral treatment. | vaginal proinflammatory cytokine expression during herpes virus reactivation was examined in human immunodeficiency virus-infected women before and after initiation of antiretroviral therapy (art). vaginal swabs were screened for levels of cytokines interleukin (il)-1β, il-2, il-4, il-6, il-8, il-10, il-12p70, il-13, tumor necrosis factor (tnf)-α, and interferon-γ. the relative risk (rr) of herpes simplex virus-2 or cytomegalovirus (cmv) shedding being associated with cytokine levels above the m ... | 2016 | 27191006 |
| [immune system data in patients with persistent recurent herpes virus infections in dynamics of complex therapy]. | the positive clinical, serolgical and immunological effects of glutamyl-triptophan in patients on persistent herpes virus infections are shown. treatment resulted in the increase of avidity on hsv 1, hsv 2, cmv, ebv antibody, the levels of cd3+-, icd8+-, cd16+-, cd3+hla-dr+- (%, abs) and.cd3+cd25t-cells (%), that indicates the optimization of the immune systemdata. the data received allow to recommend bestim for patients with persistent herpes virus infections. | 2016 | 27089710 |
| seroprevalence of cmv, hsv-2 and hbv among hiv-infected malawian children: a cross-sectional survey. | little is known about viral co-infections in african human immunodeficiency virus (hiv)-infected children. we examined the prevalence of seromarkers for cytomegalovirus (cmv), herpes simplex virus type 2 (hsv-2) and hepatitis b virus (hbv) infections among hiv-infected, antiretroviral treatment (art)-naïve children in lilongwe, malawi. | 2016 | 26884443 |
| exposure to perinatal infections and bipolar disorder: a systematic review. | bipolar disorder (bd) is a debilitating psychiatric disorder and a growing global public health issue. notwithstanding bd has been conceptualized as a neuroprogressive illness, there are some evidences to suggest a role for neurodevelopmental pathways in the patho-etiology of this disorder. evidences on the associations between perinatal infections and risk for bipolar disorder have been inconsistent across studies. here, we performed a systematic review of observational studies on the relations ... | 2016 | 26812921 |
| temporal cognitive decline associated with exposure to infectious agents in a population-based, aging cohort. | numerous cross-sectional studies have related exposure to neurotropic infectious agents with cognitive dysfunction in older adults, however, the temporal sequence is uncertain. | 2017 | 26710257 |
| decrease in seroprevalence for herpesviruses among pregnant women in finland: cross-sectional study of three time points 1992, 2002 and 2012. | maternal herpesvirus infections during pregnancy may cause fetal and neonatal infections. we investigated the seroprevalence of five herpesviruses: cytomegalovirus (cmv), herpes simplex virus (hsv) 1 and 2, varicella zoster virus (vzv) and epstein-barr virus (ebv) in randomly selected samples from pregnant finnish women from the years 1992, 2002 and 2012. over 20 years, the seroprevalences decreased significantly for cmv from 84.5% to 71.5% (p = 0.007) and hsv-1 from 69.5% to 45% (p < 0.001). th ... | 2016 | 26654892 |
| do viral infections have a role in benign paroxysmal positional vertigo? | to investigate the role of viral infection in benign paroxysmal positional vertigo (bppv). | 2015 | 26601554 |
| study of the frequency of herpesvirus infections among patients suspected aseptic meningitis in the west of iran. | aseptic meningitis is the most common type of meningitis and is characterized by meningeal inflammation that is not linked to identifiable bacterial pathogens in cerebrospinal fluid (csf). | 2015 | 26568804 |
| simultaneous quantification of the 8 human herpesviruses in allogeneic hematopoietic stem cell transplantation. | human herpesviruses may cause severe complications after allogeneic hematopoietic stem cell transplantation (hsct). however, the impact of some of these infections on transplant outcomes is still unclear. a prospective survey on the incidence and clinical features of herpesviruses infections after hsct has not yet been conducted in brazilian patients, and the impact of these infections on hsct outcome remains unclear. | 2016 | 26555946 |
| association of herpes viruses with mild, moderate and severe chronic periodontitis. | periodontal disease is an inflammatory condition of the supporting tissues of the teeth. it is a multi-factorial and multi-etiological infectious disease process. recent evidences shows that human herpes viruses could be putative pathogens. the aim of the present study was to evaluate the association of herpes viruses especially herpes simplex viruses (hsv-1 and 2), epstein-barr virus (ebv) and human cytomegalovirus (hcmv) in patients with chronic periodontitis. | 2015 | 26393126 |
| carbon nanotubes supported tyrosinase in the synthesis of lipophilic hydroxytyrosol and dihydrocaffeoyl catechols with antiviral activity against dna and rna viruses. | hydroxytyrosol and dihydrocaffeoyl catechols with lipophilic properties have been synthesized in high yield using tyrosinase immobilized on multi-walled carbon nanotubes by the layer-by-layer technique. all synthesized catechols were evaluated against a large panel of dna and rna viruses, including poliovirus type 1, echovirus type 9, herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), coxsackievirus type b3 (cox b3), adenovirus type 2 and type 5 and cytomegalovirus (cmv). ... | 2015 | 26260341 |
| performance evaluation of puritan® universal transport system (unitranz-rt™) for preservation and transport of clinical viruses. | the ability of a non-propagating microbial transport medium to maintain the viability of clinically relevant viruses was compared to a similar commercial medium to establish performance equivalence. two dilutions of stock of test viruses, namely adenovirus (adv), cytomegalovirus (cmv), echovirus type 30 (ev), herpes simplex virus (hsv) types 1 and 2, influenza a, parainfluenza 3 (piv), respiratory syncytial virus (rsv), and varicella zoster virus (vzv), were spiked into puritan® medical products ... | 2015 | 26243168 |
| lack of association between herpesvirus detection in saliva and gingivitis in hiv‑infected children. | the aims of this study were to compare the detection of human herpesviruses (hhvs) in the saliva of hiv-infected and healthy control children, and to evaluate associations between viral infection and gingivitis and immunodeficiency. saliva samples were collected from 48 hiv-infected and 48 healthy control children. clinical and laboratory data were collected during dental visits and from medical records. a trained dentist determined gingival indices and extension of gingivitis. saliva samples we ... | 2015 | 26200962 |
| seminal shedding of cmv and hiv transmission among men who have sex with men. | as in many urban areas in the united states, the largest burden of the hiv epidemic in san diego is borne by men who have sex with men (msm). using data from well-characterized hiv transmitting and non-transmitting partner pairs of msm in san diego, we calculated the population attributable risk (par) of hiv transmissions for different co-infections common among msm in this area. we found that over a third of hiv transmissions could be potentially attributed to genital shedding of cytomegaloviru ... | 2015 | 26198239 |
| animal herpesviruses and their zoonotic potential for cross-species infection. | herpesviruses of humans and animals cause severe diseases that influence not only the health and epidemiological status but are also economically important in the context of food production. the members of herpesviridae are host specific agents that also share many properties that potentially make them capable of crossing the species barriers. the objective of presented review paper was to summarize the relationship between herpesviruses of animals and humans and their zoonotic potential. in hum ... | 2015 | 26094506 |
| large-scale multiplex polymerase chain reaction assay for diagnosis of viral reactivations after allogeneic hematopoietic stem cell transplantation. | viral reactivations following hematopoietic stem cell transplantation are thought to result from the breakdown of both cell-mediated and humoral immunity. as a result, many viruses could be reactivated individually or simultaneously. using a multiplex polymerase chain reaction (pcr), we prospectively examined many kinds of viral dnas at a time in 105 patients who underwent allogeneic hematopoietic stem cell transplantation. in total, 591 whole blood samples were collected weekly from pre- to 42 ... | 2015 | 25946433 |
| rapid detection of active human cytomegalovirus infection in pregnancy using loop-mediated isothermal amplification. | understanding the association between congenital human cytomegalovirus (hcmv) infection and active maternal hcmv infection during pregnancy is important for maternal and neonatal healthcare. in the present study, a loop-mediated isothermal amplification (lamp) method was established for the detection of cmv dna from whole blood or amniotic fluid samples, using reverse transcription-quantitative polymerase chain reaction. the results of the present study demonstrated that the cmv lamp assay detec ... | 2015 | 25847382 |
| manipulation of apoptosis and necroptosis signaling by herpesviruses. | like apoptosis, necroptosis is an innate immune mechanism that eliminates pathogen-infected cells. receptor-interacting protein kinase (rip)3 (also called ripk3) mediates necrotic death by phosphorylating an executioner protein, mlkl, leading to plasma membrane leakage. the pathway is triggered against viruses that block caspase 8. in murine cmv, the viral inhibitor of caspase 8 activation prevents extrinsic apoptosis but also has the potential to unleash necroptosis. this virus encodes the vira ... | 2015 | 25828583 |
| evaluation of a viral microarray based on simultaneous extraction and amplification of viral nucleotide acid for detecting human herpesviruses and enteroviruses. | in this study, a viral microarray based assay was developed to detect the human herpesviruses and enteroviruses associated with central nervous system infections, including herpes simplex virus type 1, type 2 (hsv1 and hsv2), epstein-barr virus (ebv), cytomegalovirus (cmv), enterovirus 71 (ev71), coxsackievirus a 16 (ca16) and b 5(cb5). the dna polymerase gene of human herpesviruses and 5'-untranslated region of enteroviruses were selected as the targets to design primers and probes. human herpe ... | 2015 | 25774509 |
| vaginal cytomegalovirus shedding before and after initiation of antiretroviral therapy in rakai, uganda. | vaginal shedding of cytomegalovirus (cmv) dna was determined longitudinally among 96 women coinfected with human immunodeficiency virus (hiv), herpes simplex virus 2, and cmv starting antiretroviral therapy (art) during a placebo-controlled trial of hsv-2 suppression with acyclovir in rakai, uganda. vaginal cmv was detected in 75 of 96 women (78.0%) and 379 of 1080 individual visits (35.1%). art status, higher hiv rna viral load before art initiation, and younger age were significantly associate ... | 2015 | 25743428 |
| the association between antibodies to neurotropic pathogens and schizophrenia: a case-control study. | exposure to neurotropic pathogens has been proposed as an environmental risk factor for schizophrenia and can be evaluated by measuring pathogen-specific immunoglobulin g (igg). seroprevalence of pathogen-specific igg reflects prior exposure, whereas igg levels are associated with reactivity or reinfection. several studies have examined these parameters in schizophrenia. however, results still remain inconclusive, as several previous studies did not correct for important confounding factors. | 2015 | 27336045 |
| unpacking the 'black box' of total pathogen burden: is number or type of pathogens most predictive of all-cause mortality in the united states? | a 'black box' paradigm has prevailed in which researchers have focused on the association between the total number of pathogens for which individuals are seropositive (i.e. total pathogen burden) and various chronic diseases, while largely ignoring the role that seropositivity for specific combinations of pathogens may play in the aetiology of such outcomes and consequently mortality. we examined the association between total pathogen burden as well as specific pathogen combinations and all-caus ... | 2015 | 25518978 |
| high seroprevalence of human herpesviruses in hiv-infected individuals attending primary healthcare facilities in rural south africa. | seroprevalence data of human herpesviruses (hhvs) are limited for sub-saharan africa. these are important to provide an indication of potential burden of hhv-related disease, in particular in human immunodeficiency virus (hiv)-infected individuals who are known to be at increased risk of these conditions in the western world. in this cross-sectional study among 405 hiv-infected and antiretroviral therapy naïve individuals in rural south africa the seroprevalence of hhvs was: herpes simplex virus ... | 2014 | 24914671 |
| detection of herpesvirus, enterovirus, and arbovirus infection in patients with suspected central nervous system viral infection in the western brazilian amazon. | acute infections of the central nervous system (cns) can be caused by various pathogens. in this study, the presence of herpesviruses (hhv), enteroviruses (evs), and arboviruses were investigated in csf samples from 165 patients with suspected cns viral infection through polymerase chain reaction (pcr) and reverse transcriptase pcr. the genomes of one or more viral agents were detected in 29.7% (49/165) of the csf samples. evs were predominant (16/49; 32.6%) followed by epstein-barr virus (ebv) ... | 2014 | 24760682 |
| molecular diagnosis of central nervous system opportunistic infections in hiv-infected zambian adults. | knowledge of central nervous system (cns) opportunistic infections (ois) among people living with human immunodeficiency virus (hiv) in sub-saharan africa is limited. | 2014 | 24668125 |
| analytical characterization of an assay designed to detect and identify diverse agents of disseminated viral infection. | diverse viruses often reactivate in or infect cancer patients, patients with immunocompromising infections or genetic conditions, and transplant recipients undergoing immunosuppressive therapy. these infections can disseminate, leading to death, transplant rejection, and other severe outcomes. | 2014 | 24440177 |
| broad-range pcr-electrospray ionization mass spectrometry for detection and typing of adenovirus and other opportunistic viruses in stem cell transplant patients. | hematopoietic stem cell transplant patients are highly susceptible to viral infections. follow-up after transplantation includes weekly screening using single, virus-specific real-time pcr tests, mainly for viruses in the families herpesviridae and adenoviridae that contribute to a high morbidity, especially in pediatric populations. the abbott plex-id platform combines broad-range pcr with electrospray ionization mass spectrometry to enable the simultaneous detection of multiple pathogens in a ... | 2013 | 24108617 |
| development of recombinant antigen array for simultaneous detection of viral antibodies. | protein microarrays have been developed to study antibody reactivity against a large number of antigens, demonstrating extensive perspective for clinical application. we developed a viral antigen array by spotting four recombinant antigens and synthetic peptide, including glycoprotein g of herpes simplex virus (hsv) type 1 and 2, phosphoprotein 150 of cytomegalovirus (cmv), rubella virus (rv) core plus glycoprotein e1 and e2 as well as a e1 peptide with the optimal concentrations on activated gl ... | 2013 | 24058498 |
| seroprevalences of varicella-zoster virus, herpes simplex virus and cytomegalovirus in a cross-sectional study in mexico. | we estimated the seroprevalences of varicella-zoster virus (vzv), herpes simplex virus (hsv) and cytomegalovirus (cmv) in this cross-sectional database study. serum samples collected during the national health and nutrition survey (ensanut 2006) were obtained from subjects aged 1-70 years between january and october 2010. serological assays for the determination of antibodies against vzv, hsv and cmv were performed. the overall seroprevalences of vzv, hsv-1, hsv-2 and cmv were 85.8%, 80.9%, 9.9% ... | 2013 | 24021305 |
| laboratory diagnosis of herpesvirus infections in patients with pemphigus vulgaris lesions. | pemphigus vulgaris (pv) is an autoimmune disorder that has an etiology impacted by genetic and exogenous factors. viral infections, in particular herpesvirus infections, have been identified as possible pv triggers which in addition cause serious complications in these patients. this study investigates the frequency of herpesvirus infections in patients with pv lesions. | 2013 | 23735576 |
| herpesviruses and the microbiome. | the focus of this article will be to examine the role of common herpesviruses as a component of the microbiome of atopic patients and to review clinical observations suggesting that atopic patients might be predisposed to more severe and atypical herpes-related illness because their immune response is biased toward a th2 cytokine profile. human populations are infected with 8 herpesviruses, including herpes simplex virus hsv1 and hsv2 (also termed hhv1 and hhv2), varicella zoster virus (vzv or h ... | 2013 | 23611298 |
| frequent detection of cytomegalovirus and epstein-barr virus in cervical secretions from healthy young women. | to investigate asymptomatic shedding from the uterine cervix of five human herpes viruses: cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv) type 1 and type 2 and varicella zoster virus (vzv), in young women. | 2013 | 23550605 |
| alphaherpesvirinae and gammaherpesvirinae glycoprotein l and cmv ul130 originate from chemokines. | herpesviridae is a large family of dna viruses divided into three subfamilies: alpha-, beta- and gammaherpesvirinae. the process of herpesvirus transmission is mediated by a range of proteins, one of which is glycoprotein l (gl). based on our analysis of the solved structures of hsv2 and ebv gh/gl complexes, we propose that alphaherpesvirinae and gammaherpesvirinae glycoprotein l and betaherpesvirinae ul130 originate from chemokines. herpes simplex virus type 2 gl and human cytomegalovirus homol ... | 2013 | 23279912 |
| viral infections of the central nervous system in spain: a prospective study. | the aim of the study was to determine the incidence of viruses causing aseptic meningitis, meningoencephalitis, and encephalitis in spain. this was a prospective study, in collaboration with 17 spanish hospitals, including 581 cases (csf from all and sera from 280): meningitis (340), meningoencephalitis (91), encephalitis (76), febrile syndrome (7), other neurological disorders (32), and 35 cases without clinical information. csf were assayed by pcr for enterovirus (ev), herpesvirus (herpes simp ... | 2013 | 23239485 |
| absence of viruses in amniotic fluid of women with pprom: a case series. | preterm premature rupture of membranes (pprom) and preterm birth are strongly linked to intrauterine inflammation. bacterial infection is often not identified as the cause. the objective was to examine the amniotic fluid of women with pprom for viral genomic content, and to correlate with the presence of bacterial infection and markers of intrauterine inflammation. a case series of 13 women with pprom is presented. amniocentesis was performed in each of these women. dna/rna isolated from amnioti ... | 2012 | 23021256 |
| persistent infection with neurotropic herpes viruses and cognitive impairment. | herpes virus infections can cause cognitive impairment during and after acute encephalitis. although chronic, latent/persistent infection is considered to be relatively benign, some studies have documented cognitive impairment in exposed persons that is untraceable to encephalitis. these studies were conducted among schizophrenia (sz) patients or older community dwellers, among whom it is difficult to control for the effects of co-morbid illness and medications. to determine whether the associat ... | 2013 | 22975221 |
| maternal antibodies to infectious agents and risk for non-affective psychoses in the offspring--a matched case-control study. | an increasing number of studies suggest that certain maternal infections are associated with non-affective psychoses in the offspring. here we investigated if maternal exposure to toxoplasma gondii, cytomegalovirus (cmv), herpes simplex virus type 1 (hsv-1) or type 2 (hsv-2) prior to delivery was associated with future diagnosis of schizophrenia or other non-affective psychoses in the offspring. | 2012 | 22819777 |
| detection of herpes viruses in the cerebrospinal fluid of adults with suspected viral meningitis in malawi. | we looked for herpes simplex virus types 1 and 2 (hsv-1 and hsv-2, respectively), varicella zoster virus (vzv), epstein-barr virus (ebv) and cytomegalovirus (cmv) dna in malawian adults with clinically suspected meningitis. | 2013 | 22798048 |
| peripheral blood cd4 t-cell and plasmacytoid dendritic cell (pdc) reactivity to herpes simplex virus 2 and pdc number do not correlate with the clinical or virologic severity of recurrent genital herpes. | leukocytes participate in the immune control of herpes simplex virus (hsv). data from hiv coinfections, germ line mutations, and case reports suggest involvement of cd4 t cells and plasmacytoid dendritic cells (pdc). we investigated the relationships between these cells and recurrent genital herpes disease severity in the general population. circulating cd4 t-cell responses to hsv-2 were measured in specimens from 67 immunocompetent individuals with measured genital lesion and hsv shedding rates ... | 2012 | 22761381 |
| prenatal maternal infection, neurodevelopment and adult schizophrenia: a systematic review of population-based studies. | disruption of foetal development by prenatal maternal infection is consistent with a neurodevelopmental model of schizophrenia. whether specific prenatal infections are involved, their timing and the mechanisms of any effect are all unknown. we addressed these questions through a systematic review of population-based studies. | 2013 | 22717193 |
| infectious causes of posterior uveitis and panuveitis in thailand. | to determine the infectious causes of posterior uveitis (pu) and panuveitis (panu) in thailand. | 2012 | 22539102 |
| atypical presentation of exophytic herpes simplex virus type 2 with concurrent cytomegalovirus infection: a significant pitfall in diagnosis. | we report 3 unusual cases of atypical exophytic cutaneous herpes simplex virus (hsv) type 2 with concurrent cytomegalovirus (cmv) infection in immunosuppressed patients and raise awareness to the significant clinical and pathologic challenges in establishing the correct diagnosis. in all the 3 cases, the lesions presented as fungating plaques and nodules with areas of superficial erosion. initial clinical differential included genital warts, syphilis, versus cutaneous malignancy. all the 3 patie ... | 2013 | 22534637 |
| herpes simplex virus type 2 (hsv-2) as a coronary atherosclerosis risk factor in hiv-infected men: multicenter aids cohort study. | we assessed associations of herpes simplex virus types 1 and 2 (hsv-1 and -2), cytomegalovirus (cmv), and human herpesvirus 8 (hhv-8) infection with subclinical coronary atherosclerosis in 291 hiv-infected men in the multicenter aids cohort study. coronary artery calcium (cac) was measured by non-contrast coronary ct imaging. markers for herpesviruses infection were measured in frozen specimens collected 10-12 years prior to case identification. multivariable logistic regression models and ordin ... | 2012 | 22472456 |
| herpesviruses enhance fibrin clot lysis. | the incorporation of virus- and host-derived procoagulant factors initiates clotting directly on the surface of herpesviruses, which is an explanation for their correlation to vascular disease. the virus exploits the resulting thrombin to enhance infection by modulating the host cell through protease activated receptor (par) 1 signalling. prior reports demonstrated that at least one herpesvirus expresses surface annexin a2 (a2), a cofactor for tissue plasminogen activator (tpa)-dependent activat ... | 2012 | 22318336 |
| correlating flavivirus virulence and levels of intrinsic disorder in shell proteins: protective roles vs. immune evasion. | computational analyses revealed correlations between the intrinsic disorder propensity of shell proteins and case fatality rates (cfrs) among flaviviruses and within at least two flavivirus species, such as tick-borne encephalitis virus (tbev) and dengue virus (denv). the shell proteins analyzed in this study are capsid (c) and membrane (prm, pr, and m) proteins. the highest correlations can be found when regression analyses were conducted using pr (flavivirus: r(2) = 0.78, p < 0.01) or m (flavi ... | 2016 | 27102744 |
| viral infections in mice with reconstituted human immune system components. | pathogenic viruses are often difficult to study due to their exclusive tropism for humans. the development of mice with human immune system components opens the possibility to study those human pathogens with a tropism for the human hematopoietic lineage in vivo. these include hcmv, ebv, kshv, hiv, htlv-1, dengue virus and jc virus. furthermore, some human pathogens, like hsv-2, adenovirus, hcv, hbv and influenza a virus, with an additional tropism for somatic mouse tissues or for additional tra ... | 2014 | 24953718 |
| toxicity and antiviral activity of the extracts of submerged mycelium of nematophagous duddingtonia flagrans fungus in vero cell culture. | we studied toxicity and antiviral activity of aqueous and ethanol extracts of bioactive substances from the biomass of nematophagous fungus duddingtonia flagrans prepared by submerged culturing of the mycelium. it is found that both extracts were characterized by low toxicity for cultured vero cells and inhibited reproduction of dna-viruses in this cell line. ethanol extract of the fungus exhibited higher in vitro antiviral activity against herpes simplex virus type 2, ectromelia virus, and vacc ... | 2015 | 26621278 |
| pyrrolidine dithiocarbamate inhibits herpes simplex virus 1 and 2 replication, and its activity may be mediated through dysregulation of the ubiquitin-proteasome system. | pyrrolidine dithiocarbamate (pdtc) is widely used as an antioxidant or an nf-κb inhibitor. it has been reported to inhibit the replication of human rhinoviruses, poliovirus, coxsackievirus, and influenza virus. in this paper, we report that pdtc could inhibit the replication of herpes simplex virus 1 and 2 (hsv-1 and hsv-2). pdtc suppressed the expression of hsv-1 and hsv-2 viral immediate early (ie) and late (membrane protein gd) genes and the production of viral progeny. this antiviral propert ... | 2013 | 23740985 |
| comparison of follicular and luteal phase mucosal markers of hiv susceptibility in healthy women. | the purpose of this study was to evaluate differences in vaginal immune cell populations, vaginal tissue gene expression, antimicrobial activity of the cervicovaginal (cv) lavage (cvl), vaginal flora, and p24 antigen production from cv tissues after ex vivo human immunodeficiency virus (hiv) infection between follicular (fol) and luteal (lut) phases of the menstrual cycle. cv tissue biopsies, cv secretions, and blood samples were obtained as part of two longitudinal clinical trials of healthy wo ... | 2016 | 26750085 |
| antibacterial and antiviral evaluation of sulfonoquinovosyldiacylglyceride: a glycolipid isolated from azadirachta indica leaves. | assessment of antibacterial as well as antiherpes virus activity of sulfonoquinovosyldiacylglyceride (sqdg), a glycolipid, isolated from the leaves of azadirachta indica has been described. antimicrobial activity was evaluated against gram-positive, gram-negative bacteria and herpes simplex virus. sqdg showed significant inhibitory activity against salmonella typhi and two isolates of shigella dysenteriae with mic values 32 μg ml(-1) , while three isolates of salm. typhi, escherichia coli and vi ... | 2014 | 24118020 |
| recombinant glycoprotein g analog for determination of specific immunoglobulins to herpes simplex virus type 2 by elisa. | in order to the detection of type-specific igg to herpes simplex virus type 2 (hsv-2) in human serum or plasma the recombinant analog of hsv-2 glycoprotein g (gg2) was created. to construct an expression vector the dna fragment with a sequence identical to immunodominant regions of hsv-2 gg2 was cloned into modified vector pet28a containing of the glutation-s-transferase sequence (pet28-gst). escherichia coli bl21 (de3) were transformed with the recombinant plasmid. the target protein was expres ... | 2013 | 23933396 |
| antibiotic-free production of a herpes simplex virus 2 dna vaccine in a high yield cgmp process. | two dna vaccine plasmids encoding herpes simplex virus type 2 (hsv-2) glycoprotein d, ntc8485-o2-gd2 and ntc8485-o2-ugd2tr, were produced at large scale under current good manufacturing practice (cgmp) for use in a phase i human clinical trial. these dna vaccines incorporate the regulatory agency compliant, minimal, antibiotic-free (af) ntc8485 mammalian expression vector. plasmid yields of>1 g/l were achieved using the hypergro™ fed-batch fermentation process, with successful scale up from 10 l ... | 2013 | 23899469 |
| discovery of potential diagnostic and vaccine antigens in herpes simplex virus 1 and 2 by proteome-wide antibody profiling. | routine serodiagnosis of herpes simplex virus (hsv) infections is currently performed using recombinant glycoprotein g (gg) antigens from herpes simplex virus 1 (hsv-1) and hsv-2. this is a single-antigen test and has only one diagnostic application. relatively little is known about hsv antigenicity at the proteome-wide level, and the full potential of mining the antibody repertoire to identify antigens with other useful diagnostic properties and candidate vaccine antigens is yet to be realized. ... | 2012 | 22318154 |
| a lentiviral vector-based, herpes simplex virus 1 (hsv-1) glycoprotein b vaccine affords cross-protection against hsv-1 and hsv-2 genital infections. | genital herpes is caused by herpes simplex virus 1 (hsv-1) and hsv-2, and its incidence is constantly increasing in the human population. regardless of the clinical manifestation, hsv-1 and hsv-2 infections are highly transmissible to sexual partners and enhance susceptibility to other sexually transmitted infections. an effective vaccine is not yet available. here, hsv-1 glycoprotein b (gb1) was delivered by a feline immunodeficiency virus (fiv) vector and tested against hsv-1 and hsv-2 vaginal ... | 2012 | 22491465 |
| antiviral activity of basidiomycete mycelia against influenza type a (serotype h1n1) and herpes simplex virus type 2 in cell culture. | in this study, we investigated the in vitro antiviral activity of the mycelia of higher mushrooms against influenza virus type a (serotype h1n1) and herpes simplex virus type 2 (hsv-2), strain bh. all 10 investigated mushroom species inhibited the reproduction of influenza virus strain a/fm/1/47 (h1n1) in mdck cells reducing the infectious titer by 2.0-6.0 lg id50. four species, pleurotus ostreatus, fomes fomentarius, auriporia aurea, and trametes versicolor, were also determined to be effective ... | 2014 | 25358999 |
| design, synthesis, antiviral and cytostatic activity of ω-(1h-1,2,3-triazol-1-yl)(polyhydroxy)alkylphosphonates as acyclic nucleotide analogues. | the efficient synthesis of a new series of polyhydroxylated dibenzyl ω-(1h-1,2,3-triazol-1-yl)alkylphosphonates as acyclic nucleotide analogues is described starting from dibenzyl ω-azido(polyhydroxy)alkylphosphonates and selected alkynes under microwave irradiation. selected o,o-dibenzylphosphonate acyclonucleotides were transformed into the respective phosphonic acids. all compounds were evaluated in vitro for activity against a broad variety of dna and rna viruses and for cytostatic activity ... | 2014 | 24906510 |
| a multicenter prospective trial to asses a new real-time polymerase chain reaction for detection of treponema pallidum, herpes simplex-1/2 and haemophilus ducreyi in genital, anal and oropharyngeal ulcers. | treponema pallidum, herpes simplex virus types 1 or 2 (hsv-1/2) and haemophilus ducreyi are sexually transmitted pathogens that can cause genital, anal and oropharyngeal ulcers. laboratory evaluation of these pathogens in ulcers requires different types of specimens and tests, increasing the risk of improper specimen handling and time lapse until analysis. we sought to develop a new real-time pcr (tp-hd-hsv1/2 pcr) to facilitate the detection of t. pallidum, hsv-1/2 and h. ducreyi in ulcers. the ... | 2014 | 24909546 |
| etiology of genital ulcer disease and association with hiv infection in malawi. | the world health organization recommends the use of syndromic management for patients presenting with genital ulcer disease (gud) in developing countries. however, effective treatment guidelines depend on a current country-specific gud etiological profile, which may change over time. | 2013 | 24220352 |
| hiv and sti s among men who have sex with men in dodoma municipality, tanzania: a cross-sectional study. | to determine the seroprevalence of hiv, sti and related risks among men who have sex with men (msm) in dodoma municipality, tanzania. | 2017 | 28202736 |
| does place of service matter? a utilisation and cost analysis of sexually transmissible infection testing from 2012 claims data. | background in this study, a previous study on the utilisation and cost of sexually transmissible infection (sti) tests was augmented by focusing on outpatient place of service for the most utilised tests. | 2016 | 26774890 |
| antigenic breadth: a missing ingredient in hsv-2 subunit vaccines? | the successful human papillomavirus and hepatitis b virus subunit vaccines contain single viral proteins that represent 22 and 12%, respectively, of the antigens encoded by these tiny viruses. the herpes simplex virus 2 (hsv-2) genome is >20 times larger. thus, a single protein subunit represents 1% of hsv-2's total antigenic breadth. antigenic breadth may explain why hsv-2 glycoprotein subunit vaccines have failed in clinical trials, and why live hsv-2 vaccines that express 99% of hsv-2's prote ... | 2014 | 24837838 |
| toward global prevention of sexually transmitted infections (stis): the need for sti vaccines. | an estimated 499 million curable sexually transmitted infections (stis; gonorrhea, chlamydia, syphilis, and trichomoniasis) occurred globally in 2008. in addition, well over 500 million people are estimated to have a viral sti such as herpes simplex virus type 2 (hsv-2) or human papillomavirus (hpv) at any point in time. stis result in a large global burden of sexual, reproductive, and maternal-child health consequences, including genital symptoms, pregnancy complications, cancer, infertility, a ... | 2014 | 24581979 |
| utilization and cost of diagnostic methods for sexually transmitted infection screening among insured american youth, 2008. | private sector utilization and cost information on testing for sexually transmitted infections (stis) in the united states is limited. | 2013 | 23588123 |
| the estimated direct medical cost of selected sexually transmitted infections in the united states, 2008. | millions of cases of sexually transmitted infections (stis) occur in the united states each year, resulting in substantial medical costs to the nation. previous estimates of the total direct cost of stis are quite dated. we present updated direct medical cost estimates of stis in the united states. | 2013 | 23403600 |
| tin oxide nanowires suppress herpes simplex virus-1 entry and cell-to-cell membrane fusion. | the advent of nanotechnology has ushered in the use of modified nanoparticles as potential antiviral agents against diseases such as herpes simplex virus 1 and 2 (hsv-1) (hsv-2), human immunodeficiency virus (hiv), monkeypox virus, and hepatitis b virus. here we describe the application of tin oxide (sno(2)) nanowires as an effective treatment against hsv-1 infection. sno(2) nanowires work as a carrier of negatively charged structures that compete with hsv-1 attachment to cell bound heparan sulf ... | 2012 | 23110193 |
| parallel declines in hiv and hepatitis c virus prevalence, but not in herpes simplex virus type 2 infection: a 10-year, serial cross-sectional study in an inner-city emergency department. | the johns hopkins hospital emergency department (jhhed) has served as an observational window on the hiv epidemic in a socioeconomically depressed, urban population. we previously reported that hiv incidence among jhhed patients is decreasing and that prevalence has declined from 11.4% in 2003-5.6% in 2013. | 2016 | 27232485 |
| high prevalence of herpes simplex virus (hsv)- type 2 co-infection among hiv-positive women in ukraine, but no increased hiv mother-to-child transmission risk. | over 3500 hiv-positive women give birth annually in ukraine, a setting with high prevalence of sexually transmitted infections. herpes simplex virus type 2 (hsv-2) co-infection may increase hiv mother-to-child transmission (mtct) risk. we explored factors associated with hsv-2 seropositivity among hiv-positive women in ukraine, and its impact on hiv mtct. | 2016 | 27121953 |
| interferon lambda 4 genotype is not associated with recurrence of oral or genital herpes. | ifnl4-δg/tt (rs368234815) genotype is associated with hepatitis c virus clearance and may play a role in other infections. ifn-λ4 protein is generated only in individuals who carry the ifnl4-δg allele. the ifnl4 rs12979860-t allele, which is in strong linkage disequilibrium with ifnl4-δg, was recently reported to be associated with more frequent and severe oral herpes episodes. we investigated the association of ifnl4-δg/tt with herpes simplex virus (hsv)-related outcomes among 2,192 african ame ... | 2015 | 26431156 |
| a pilot study examining the safety and tolerability of valacyclovir in veterans with hepatitis c virus/herpes simplex virus type 2 coinfection. | we performed a pilot study examining the safety and tolerability of valacyclovir in veterans with herpes simplex virus type 2 and hepatitis c virus (hcv) coinfection. | 2014 | 25163019 |
| coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed hiv-infected patients. | infection with co-pathogens is one of the postulated factors contributing to persistent inflammation and non-aids events in virologically-suppressed hiv-infected patients. we aimed to investigate the relationship of human herpesvirus-8 (hhv-8), a vasculotropic virus implicated in the pathogenesis of kaposi's sarcoma, with inflammation and subclinical atherosclerosis in hiv-infected patients. | 2014 | 25133669 |
| risky behavior and correlates of hiv and hepatitis c virus infection among people who inject drugs in three cities in afghanistan. | injecting drug use is the primary mode of hiv transmission and acquisition in afghanistan. people who inject drugs (pwid) in the country have been characterized by high risk injecting behavior and a high burden of hcv infection. we aimed to estimate the burden of hiv, hcv, and other infectious diseases and to identify the correlates of hiv and hcv infection among pwid living in three major afghan cities in 2009. | 2014 | 25131717 |
| alarming epidemics of human immunodeficiency virus and hepatitis c virus among injection drug users in the northwestern bordering state of punjab, india: prevalence and correlates. | in this study 1155 injection drug users (idus) receiving targeted intervention in five cities of punjab were surveyed; three cities (amritsar, taran-taran, batala) were in districts sharing an international border with pakistan and the other two were jalandhar and ludhiana. information on socio-demography, substance use and sexual practices were collected; blood samples were tested for biologic markers. hiv and hcv antibody (hcvab) prevalences in injection drug users were 29 and 49%, respectivel ... | 2014 | 24352120 |
| individual and network factors associated with prevalent hepatitis c infection among rural appalachian injection drug users. | we determined the factors associated with hepatitis c (hcv) infection among rural appalachian drug users. | 2013 | 23153148 |