| the biosynthesis of udp-d-quinac in bacillus cereus atcc 14579. | n-acetylquinovosamine (2-acetamido-2,6-di-deoxy-d-glucose, quinac) is a relatively rare amino sugar residue found in glycans of few pathogenic gram-negative bacteria where it can play a role in infection. however, little is known about quinac-related polysaccharides in gram-positive bacteria. in a routine screen for bacillus glycan grown at defined medium, it was surprising to identify a quinac residue in polysaccharides isolated from this gram-positive bacterium. to gain insight into the biosyn ... | 2015 | 26207987 |
| proteolytic activities expressed by gastrointestinal pathogens bacillus cereus, listeria monocytogenes and enterococcus faecium in different growth phases. | bacterial proteases are implicated in protein quality control, biofilm formation or might have a direct function in pathogenesis by processing virulence factors or cleaving host factors. in recent years, knowledge of proteases expressed by gram-negative pathogens remarkably increased. however, investigation of proteases from gram-positive bacteria is rather rare, but required for the analysis of pathogenesis-relevant proteases. in this study, we extracted and detected proteases from the gastroin ... | 2015 | 26682199 |
| the bacterial dna repair protein mfd confers resistance to the host nitrogen immune response. | production of reactive nitrogen species (no) is a key step in the immune response following infections. no induces lesions to bacterial dna, thus limiting bacterial growth within hosts. using two pathogenic bacteria, bacillus cereus and shigella flexneri, we show that the dna-repair protein mfd (mutation-frequency-decline) is required for bacterial resistance to the host-no-response. in both species, a mutant deficient for mfd does not survive to no, produced in vitro or by phagocytic cells. in ... | 2016 | 27435260 |
| phenolic compounds and in vitro antibacterial and antioxidant activities of three tropic fruits: persimmon, guava, and sweetsop. | in our previous study, we have found that persimmon, guava, and sweetsop owned considerably high antioxidant activity and contained high total phenolic contents as well. in order to further supply information on the antibacterial and antioxidant activity of these three tropic fruits, they were extracted by 80% methanol. we then examined the extractions about their phenolic compounds and also studied the extractions and phenolic contents about their minimum inhibitory concentration (mic) and mini ... | 2016 | 27648444 |
| methylatable signaling helix coordinated inhibitory receiver domain in sensor kinase modulates environmental stress response in bacillus cereus. | σb, an alternative transcription factor, controls the response of the cell to a variety of environmental stresses in bacillus cereus. previously, we reported that rsbm negatively regulates σb through the methylation of rsbk, a hybrid sensor kinase, on a signaling helix (s-helix). however, rsbk comprises a c-terminal receiver (rec) domain whose function remains unclear. in this study, deletion of the c-terminal rec domain of rsbk resulted in high constitutive σb expression independent of environm ... | 2015 | 26379238 |
| identification of cdnl, a putative transcriptional regulator involved in repair and outgrowth of heat-damaged bacillus cereus spores. | spores are widely present in the environment and are common contaminants in the food chain, creating a challenge for food industry. nowadays, heat treatments conventionally applied in food processing may become milder to comply with consumer desire for products with higher sensory and nutritional values. consequently subpopulations of spores may emerge that are sublethally damaged rather than inactivated. such spores may germinate, repair damage, and eventually grow out leading to uncontrolled s ... | 2016 | 26849219 |
| biocidal and sporicidal efficacy of pathoster(®) 0.35% and pathoster(®) 0.50% against bacterial agents in potential bioterrorism use. | the use of products that can neutralize or significantly reduce the microbial load and that are not harmful to human health and the environment represents a milestone in the fight against the spread of infectious diseases. peracetic acid, besides being an excellent sterilizing and sporicidal agent, is harmless to humans and the environment when it is used in a common dosage. however, the high costs and loss of efficacy of the product very quickly after its reconstitution limit its use. we evalua ... | 2016 | 27482880 |
| novel highly thermostable endolysin from thermus scotoductus mat2119 bacteriophage ph2119 with amino acid sequence similarity to eukaryotic peptidoglycan recognition proteins. | in this study, we present the discovery and characterization of a highly thermostable endolysin from bacteriophage ph2119 infecting thermus strain mat2119 isolated from geothermal areas in iceland. nucleotide sequence analysis of the 16s rrna gene affiliated the strain with the species thermus scotoductus. bioinformatics analysis has allowed identification in the genome of phage 2119 of an open reading frame (468 bp in length) coding for a 155-amino-acid basic protein with an mr of 17,555. ph211 ... | 2014 | 24271162 |
| adaptation in bacillus cereus: from stress to disease. | bacillus cereus is a food-borne pathogen that causes diarrheal disease in humans. after ingestion, b. cereus experiences in the human gastro-intestinal tract abiotic physical variables encountered in food, such as acidic ph in the stomach and changing oxygen conditions in the human intestine. b. cereus responds to environmental changing conditions (stress) by reversibly adjusting its physiology to maximize resource utilization while maintaining structural and genetic integrity by repairing and m ... | 2016 | 27757102 |
| structural basis of the substrate specificity of bacillus cereus adenosine phosphorylase. | purine nucleoside phosphorylases catalyze the phosphorolytic cleavage of the glycosidic bond of purine (2'-deoxy)nucleosides, generating the corresponding free base and (2'-deoxy)-ribose 1-phosphate. two classes of pnps have been identified: homotrimers specific for 6-oxopurines and homohexamers that accept both 6-oxopurines and 6-aminopurines. bacillus cereus adenosine phosphorylase (adop) is a hexameric pnp; however, it is highly specific for 6-aminopurines. to investigate the structural basis ... | 2012 | 22349225 |
| phages preying on bacillus anthracis, bacillus cereus, and bacillus thuringiensis: past, present and future. | many bacteriophages (phages) have been widely studied due to their major role in virulence evolution of bacterial pathogens. however, less attention has been paid to phages preying on bacteria from the bacillus cereus group and their contribution to the bacterial genetic pool has been disregarded. therefore, this review brings together the main information for the b. cereus group phages, from their discovery to their modern biotechnological applications. a special focus is given to phages infect ... | 2014 | 25010767 |
| differential sensitivity of polyhydroxyalkanoate producing bacteria to fermentation inhibitors and comparison of polyhydroxybutyrate production from burkholderia cepacia and pseudomonas pseudoflava. | the aim of this study is determine the relative sensitivity of a panel of seven polyhydroxyalkanoate producing bacteria to a panel of seven lignocellulosic-derived fermentation inhibitors representing aliphatic acids, furans and phenolics. a further aim was to measure the polyhydroxybutyrate production of select organisms on lignocellulosic-derived monosaccharides arabinose, xylose, glucose and mannose. | 2013 | 23734728 |
| identification of pbc218/pbc210 genes of bacillus cereus g9241 in five florida soils using qpcr. | the distribution of the virulent plasmid pbc210 of b. cereus that carries several b. anthracis genes and has been implicated in lethal anthrax-like pulmonary disease is unknown. we screened our collection of 103 b. cereus isolates and 256 soil samples using a quantitative pcr (qpcr) assay that targeted three open reading frames putatively unique to pbc210. when tested with dna from 2 b. cereus strains carrying pbc210, and 64 gram-positive and 55 gram-negative bacterial species, the assay had 100 ... | 2014 | 27350995 |
| chemical composition and antimicrobial activity of hexane leaf extract of anisopus mannii (asclepiadaceae). | the aim was to determine the chemical constituents and antimicrobial activity of the hexane leaf extract of anisopus mannii against a wide range of human pathogenic microorganisms. | 2015 | 26401399 |
| deciphering a survival strategy during the interspecific competition between bacillus cereus msm-s1 and pseudomonas sp. msm-m1. | interspecific competition in bacteria governs colony growth dynamics and pattern formation. here, we demonstrate an interesting phenomenon of interspecific competition between bacillus cereus msm-s1 and pseudomonas sp. msm-m1, where secretion of an inhibitor by pseudomonas sp. is used as a strategy for survival. although b. cereus grows faster than pseudomonas sp., in the presence of pseudomonas sp. the population of b. cereus reduces significantly, whereas pseudomonas sp. do not show any marked ... | 2016 | 28018625 |
| control of wilt and rot pathogens of tomato by antagonistic pink pigmented facultative methylotrophic delftia lacustris and bacillus spp. | the studies on the biocontrol potential of pink pigmented facultative methylotrophic (ppfm) bacteria other than the genus methylobacterium are scarce. in the present study, we report three facultative methylotrophic isolates; ppo-1, ppt-1, and ppb-1, respectively, identified as delftia lacustris, bacillus subtilis, and bacillus cereus by 16s rrna gene sequence analysis. hemolytic activity was tested to investigate the potential pathogenicity of isolates to plants and humans, the results indicate ... | 2016 | 27872630 |
| rhizospheric bacteria of maize with potential for biocontrol of fusarium verticillioides. | the stalk, ear and root rot (serr) of maize caused by fusarium verticillioides (fv) severely impacts crop production in tropical and subtropical regions. the aim of the present work was to screen bacterial isolates in order to find novel native biocontrol agents against fv. a culturable bacterial collection consisting of 11,520 isolates enriched in firmicutes and proteobacteria was created from rhizosphere samples taken from serr symptomatic or asymptomatic maize plants. the complete collection ... | 2016 | 27066355 |
| phytase, phosphatase activity and p-nutrition of soybean as influenced by inoculation of bacillus. | the efficiency of different bacillus isolates on rhizosphere soil enzyme activities and p-nutrition of soybean was carried out under microcosm conditions. significant increase in enzyme activities viz., fluorescein diacetate activity, phosphatase and phytase activity and consequent effects on p-nutrition were observed with the inoculation of bacillus isolates over uninoculated control. among the isolates, bd-3-1b, khbd-6, bdkh-3, bacillus amyloliquefacians, and bacillus cereus were found to be p ... | 2011 | 22282635 |
| influence of inoculation with plant growth promoting rhizobacteria (pgpr) on tomato plant growth and nematode reproduction under greenhouse conditions. | numerous species of soil bacteria which flourish in the rhizosphere of plants or around plant tissues stimulate plant growth and reduce nematode population by antagonistic behavior. these bacteria are collectively known as pgpr (plant growth promoting rhizobacteria). the effects of six isolates of pgpr pseudomonas putida, pseudomonas fluorescens, serratia marcescens, bacillus amyloliquefaciens, bacillus subtilis and bacillus cereus, were studied on tomato plant growth and root knot nematode repr ... | 2012 | 23961220 |
| antimicrobial activity of bacteriocin-producing lactic acid bacteria isolated from cheeses and yogurts. | the biopreservation of foods using bacteriocinogenic lactic acid bacteria (lab) isolated directly from foods is an innovative approach. the objectives of this study were to isolate and identify bacteriocinogenic lab from various cheeses and yogurts and evaluate their antimicrobial effects on selected spoilage and pathogenic microorganisms in vitro as well as on a food commodity.lab were isolated using mrs and m17 media. the agar diffusion bioassay was used to screen for bacteriocin or bacterioci ... | 2012 | 22963659 |
| the influence of interfering substances on the antimicrobial activity of selected quaternary ammonium compounds. | standard cleaning processes may not remove all the soiling typically found in food industry, such as carbohydrates, fats, or proteins. contaminants have a high impact in disinfection as their presence may reduce the activity of disinfectants. the influence of alginic acid, bovine serum albumin, yeast extract, and humic acids was assessed on the antimicrobial activities of benzalkonium chloride and cetyltrimethyl ammonium bromide against bacillus cereus vegetative cells and pseudomonas fluorescen ... | 2013 | 26904590 |
| the cyclic antibacterial peptide enterocin as-48: isolation, mode of action, and possible food applications. | enterocin as-48 is a circular bacteriocin produced by enterococcus. it contains a 70 amino acid-residue chain circularized by a head-to-tail peptide bond. the conformation of enterocin as-48 is arranged into five alpha-helices with a compact globular structure. enterocin as-48 has a wide inhibitory spectrum on gram-positive bacteria. sensitivity of gram-negative bacteria increases in combination with outer-membrane permeabilizing treatments. eukaryotic cells are bacteriocin-resistant. this catio ... | 2014 | 25493478 |
| antibacterial activity of cinnamaldehyde and clove oil: effect on selected foodborne pathogens in model food systems and watermelon juice. | natural additives for the control of microbial growth are in demand because consumers prefer them over synthetic ones. in the present investigation, the antibacterial activity of two natural preservatives, cinnamaldeyde and clove oil alone or in combinations was studied, and their potential as food preservative in model food systems and watermelon juice was evaluated. the cinnamaldehyde and clove essential oil showed minimum inhibitory concentration (mic) at or below 5000 mg/l, and fractional in ... | 2014 | 26344998 |
| selection of enhanced antimicrobial activity posing lactic acid bacteria characterised by (gtg)5-pcr fingerprinting. | the aim of the study was a detail evaluation of genetic diversity among the lactic acid bacteria (lab) strains having an advantage of a starter culture in order to select genotypically diverse strains with enhanced antimicrobial effect on some harmfull and pathogenic microorganisms. antimicrobial activity of lab was performed by the agar well diffusion method and was examined against the reference strains and foodborne isolates of bacillus cereus, listeria monocytogenes, escherichia coli, staphy ... | 2014 | 26139877 |
| a halotolerant thermostable lipase from the marine bacterium oceanobacillus sp. pumb02 with an ability to disrupt bacterial biofilms. | a halotolerant thermostable lipase was purified and characterized from the marine bacterium oceanobacillus sp. pumb02. this lipase displayed a high degree of stability over a wide range of conditions including ph, salinity, and temperature. it was optimally active at 30 °c and ph 8.0 respectively and was stable at higher temperatures (50-70 °c) and alkaline ph. the molecular mass of the lipase was approximately 31 kda based on sds-page and maldi-tof fingerprint analysis. conditions for enhanced ... | 2014 | 25482232 |
| cyclic lipodepsipeptides produced by pseudomonas spp. naturally present in raw milk induce inhibitory effects on microbiological inhibitor assays for antibiotic residue screening. | two pseudomonas strains, identified as closely related to pseudomonas tolaasii, were isolated from milk of a farm with frequent false-positive delvotest results for screening putative antibiotic residues in raw milk executed as part of the regulatory quality programme. growth at 5 to 7°c of these isolates in milk resulted in high lipolysis and the production of bacterial inhibitors. the two main bacterial inhibitors have a molecular weight of 1168.7 and 1140.7 da respectively, are heat-tolerant ... | 2014 | 24853676 |
| the self-assembly of a cyclic lipopeptides mixture secreted by a b. megaterium strain and its implications on activity against a sensitive bacillus species. | cyclic lipopeptides are produced by a soil bacillus megaterium strain and several other bacillus species. in this work, they are detected both in the bacillus intact cells and the cells culture medium by maldi-tof mass spectrometry. the cyclic lipopeptides self-assemble in water media producing negatively charged and large aggregates (300-800 nm of mean hydrodynamic radius) as evaluated by dynamic light scattering and zeta-potential analysis. the aggregate size depends on ph and ionic strength. ... | 2014 | 24816927 |
| bacteria associated with amblyomma cajennense tick eggs. | ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ... | 2015 | 26537602 |
| inhibition of pathogenic and spoilage bacteria by a novel biofilm-forming lactobacillus isolate: a potential host for the expression of heterologous proteins. | bacterial biofilms are a preferred mode of growth for many types of microorganisms in their natural environments. the ability of pathogens to integrate within a biofilm is pivotal to their survival. the possibility of biofilm formation in lactobacillus communities is also important in various industrial and medical settings. lactobacilli can eliminate the colonization of different pathogenic microorganisms. alternatively, new opportunities are now arising with the rapidly expanding potential of ... | 2015 | 26150120 |
| antimicrobial and antioxidant activity of chitosan/hydroxypropyl methylcellulose film-forming hydrosols hydrolyzed by cellulase. | the aim of this study was to evaluate the impact of cellulase (c) on the biological activity of chitosan/hydroxypropyl methylcellulose (ch/hpmc) film-forming hydrosols. the hydrolytic activity of cellulase in two concentrations (0.05% and 0.1%) was verified by determination of the progress of polysaccharide hydrolysis, based on viscosity measurement and reducing sugar-ends assay. the 2,2-diphenyl-1-picrylhydrazyl (dpph) free radical scavenging effect, the ferric reducing antioxidant power (frap) ... | 2016 | 27608008 |
| the pesticidal cry6aa toxin from bacillus thuringiensis is structurally similar to hlye-family alpha pore-forming toxins. | the cry6 family of proteins from bacillus thuringiensis represents a group of powerful toxins with great potential for use in the control of coleopteran insects and of nematode parasites of importance to agriculture. these proteins are unrelated to other insecticidal toxins at the level of their primary sequences and the structure and function of these proteins has been poorly studied to date. this has inhibited our understanding of these toxins and their mode of action, along with our ability t ... | 2016 | 27576487 |
| characterization of antimicrobial substance from lactobacillus salivarius kl-d4 and its application as biopreservative for creamy filling. | lactobacillus salivarius kl-d4 isolated from duck intestine produced bacteriocin which was stable at high temperature and a wide ph range of 3-10. its cell free supernatant at ph 5.5 exhibited wide inhibitory spectrum against both g+ and g- bacteria. the highest bacteriocin production was obtained in mrs broth supplemented with 0.5 % (w/v) caco3 at 6 h by gentle shaking. pcr walking using specific primers at the conserved region of class-ii bacteriocin resulted in 4 known genes of kld1, kld2, kl ... | 2016 | 27462508 |
| bacillus cereus biofilms-same, only different. | bacillus cereus displays a high diversity of lifestyles and ecological niches and include beneficial as well as pathogenic strains. these strains are widespread in the environment, are found on inert as well as on living surfaces and contaminate persistently the production lines of the food industry. biofilms are suspected to play a key role in this ubiquitous distribution and in this persistency. indeed, b. cereus produces a variety of biofilms which differ in their architecture and mechanism o ... | 2016 | 27458448 |
| bacillus cereus strain s2 shows high nematicidal activity against meloidogyne incognita by producing sphingosine. | plant-parasitic nematodes cause serious crop losses worldwidely. this study intended to discover the antagonistic mechanism of bacillus cereus strain s2 against meloidogyne incognita. treatment with b. cereus strain s2 resulted in a mortality of 77.89% to caenorhabditis elegans (a model organism) and 90.96% to m. incognita. in pot experiment, control efficiency of b. cereus s2 culture or supernatants were 81.36% and 67.42% towards m. incognita, respectively. in field experiment, control efficien ... | 2016 | 27338781 |
| increased biomass production by mesophilic food-associated bacteria through lowering the growth temperature from 30°c to 10°c. | five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species escherichia coli, listeria monocytogenes, staphylococcus xylosus, bacillus cereus, pedobacter nutrimenti, and pedobacter panaciterrae were tested for the effect of growth temperature (30°c and 10°c) on biomass formation. growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and define ... | 2016 | 27084015 |
| bacillus cereus ar156 extracellular polysaccharides served as a novel micro-associated molecular pattern to induced systemic immunity to pst dc3000 in arabidopsis. | non-host resistance (nhr) is a broad-spectrum plant defense. upon colonizing on the surface on the root or leaves of non-host species, pathogens initial encounter preform and induce defense response in plant, such as induced hypersensitive response, pamps triggered immunity (pti), and effector triggered immunity (eti). the ability of plants to develop an induced systemic response (isr) in reaction to the colonization by non-pathogenic rhizobacterium depends on interactions between host plants an ... | 2016 | 27242694 |
| induction of drought tolerance in cucumber plants by a consortium of three plant growth-promoting rhizobacterium strains. | our previous work showed that a consortium of three plant growth-promoting rhizobacterium (pgpr) strains (bacillus cereus ar156, bacillus subtilis sm21, and serratia sp. xy21), termed as bbs for short, was a promising biocontrol agent. the present study investigated its effect on drought tolerance in cucumber plants. after withholding watering for 13 days, bbs-treated cucumber plants had much darker green leaves and substantially lighter wilt symptoms than control plants. compared to the control ... | 2012 | 23285089 |
| the bioc o-methyltransferase catalyzes methyl esterification of malonyl-acyl carrier protein, an essential step in biotin synthesis. | recent work implicated the escherichia coli bioc protein as the initiator of the synthetic pathway that forms the pimeloyl moiety of biotin (lin, s., hanson, r. e., and cronan, j. e. (2010) nat. chem. biol. 6, 682-688). bioc was believed to be an o-methyltransferase that methylated the free carboxyl of either malonyl-coa or malonyl-acyl carrier protein based on the ability of o-methylated (but not unmethylated) precursors to bypass the bioc requirement for biotin synthesis both in vivo and in vi ... | 2012 | 22965231 |
| gram-positive siderophore-shuttle with iron-exchange from fe-siderophore to apo-siderophore by bacillus cereus yxeb. | small molecule iron-chelators, siderophores, are very important in facilitating the acquisition of fe(iii), an essential element for pathogenic bacteria. many gram-negative outer-membrane transporters and gram-positive lipoprotein siderophore-binding proteins have been characterized, and the binding ability of outer-membrane transporters and siderophore-binding proteins for fe-siderophores has been determined. however, there is little information regarding the binding ability of these proteins f ... | 2013 | 23924612 |
| a genomic region involved in the formation of adhesin fibers in bacillus cereus biofilms. | bacillus cereus is a bacterial pathogen that is responsible for many recurrent disease outbreaks due to food contamination. spores and biofilms are considered the most important reservoirs of b. cereus in contaminated fresh vegetables and fruits. biofilms are bacterial communities that are difficult to eradicate from biotic and abiotic surfaces because of their stable and extremely strong extracellular matrix. these extracellular matrixes contain exopolysaccharides, proteins, extracellular dna, ... | 2014 | 25628606 |
| identification of multidrug-resistant bacteria and bacillus cereus from healthcare workers and environmental surfaces in a hospital. | | 2014 | 25280977 |
| isolation and characterization of broad spectrum coaggregating bacteria from different water systems for potential use in bioaugmentation. | the bridging bacteria with broad-spectrum coaggregation ability play an important role during multispecies-biofilm development. in this study, through a visual and semi-quantitative assay, twenty-two bacterial strains with aggregation ability were obtained from 8 different water environments, and these strains were assigned to 7 genera according to their 16s rdna and they were aeromonas, bacillus, comamonas, exiguobacterium, pseudomonas, shewanella and comamonas. furthermore, all possible 231 pa ... | 2014 | 24736645 |
| production of polypeptide antibiotic from streptomyces parvulus and its antibacterial activity. | a highly potent secondary metabolite producing actinomycetes strain is isolated from marine soil sediments of visakhapatnam sea coast, bay of bengal. over all ten strains are isolated from the collected soil sediments. among the ten actinomycetes strains the broad spectrum strain rspsn2 was selected for molecular characterization, antibiotic production and its purification. the nucleotide sequence of the 1 rrna gene (1261 base pairs) of the most potent strain evidenced a 96% similarity with stre ... | 2014 | 24948949 |
| identification of 3-sulfinopropionyl coenzyme a (coa) desulfinases within the acyl-coa dehydrogenase superfamily. | in a previous study, the essential role of 3-sulfinopropionyl coenzyme a (3sp-coa) desulfinase acyl-coa dehydrogenase (acd) in advenella mimigardefordensis strain dpn7(t) (acddpn7) during degradation of 3,3'-dithiodipropionic acid (dtdp) was elucidated. dtdp is a sulfur-containing precursor substrate for biosynthesis of polythioesters (ptes). acddpn7 showed high amino acid sequence similarity to acyl-coa dehydrogenases but was unable to catalyze a dehydrogenation reaction. hence, it was investig ... | 2014 | 24317404 |
| alcoholytic cleavage of polyhydroxyalkanoate chains by class iv synthases induced by endogenous and exogenous ethanol. | polyhydroxyalkanoate (pha)-producing bacillus strains express class iv pha synthase, which is composed of the subunits phar and phac. recombinant escherichia coli expressing pha synthase from bacillus cereus strain yb-4 (pharcyb-4) showed an unusual reduction of the molecular weight of pha produced during the stationary phase of growth. nuclear magnetic resonance analysis of the low-molecular-weight pha revealed that its carboxy end structure was capped by ethanol, suggesting that the molecular ... | 2014 | 24334666 |
| three novel lantibiotics, ticins a1, a3, and a4, have extremely stable properties and are promising food biopreservatives. | lantibiotics are antimicrobial peptides with potential applications as the next generation of antimicrobials in the food industry and/or the pharmaceutical industry. nisin has successfully been used as a food preservative for over 40 years, but its major drawback is its limited stability under neutral and alkaline ph conditions. to identify alternatives with better biochemical properties, we screened more than 100 strains of the bacillus cereus group. three novel lantibiotics, ticins a1 (4,062.9 ... | 2015 | 26231642 |
| effects of temperature and ph on the activities of catechol 2,3-dioxygenase obtained from crude oil contaminated soil in ilaje, ondo state, nigeria. | enrichment technique was employed for the isolation of the crude oil degrading bacteria. the isolated bacteria were screened for their degradative ability and the best degrading bacteria were selected based on their growth. specific activities of catechol-2,3-dioxygenase and effects of temperature and ph and their stabilities on the enzyme relative activities were observed. bacteria isolated from the soil sample include; bacillus cereus, b. amyloliquficiens, b. firmus, acinetobacter calcoaceticu ... | 2015 | 26464607 |
| the bacillus cereus group is an excellent reservoir of novel lanthipeptides. | lantibiotics are ribosomally synthesized peptides that contain multiple posttranslational modifications. research on lantibiotics has increased recently, mainly due to their broad-spectrum antimicrobial activity, especially against some clinical gram-positive pathogens. many reports about various bacteriocins in the bacillus cereus group have been published, but few were about lantibiotics. in this study, we identified 101 putative lanthipeptide gene clusters from 77 out of 223 strains of this g ... | 2015 | 25548056 |
| flhf is required for swarming motility and full pathogenicity of bacillus cereus. | besides sporulation, bacillus cereus can undergo a differentiation process in which short swimmer cells become elongated and hyperflagellated swarmer cells that favor migration of the bacterial community on a surface. the functionally enigmatic flagellar protein flhf, which is the third paralog of the signal recognition particle (srp) gtpases ffh and ftsy, is required for swarming in many bacteria. previous data showed that flhf is involved in the control of the number and positioning of flagell ... | 2016 | 27807433 |
| listeria monocytogenes dna glycosylase adlp affects flagellar motility, biofilm formation, virulence, and stress responses. | the temperature-dependent alteration of flagellar motility gene expression is critical for the foodborne pathogen listeria monocytogenes to respond to a changing environment. in this study, a genetic determinant, l. monocytogenes f2365_0220 (lmof2365_0220), encoding a putative protein that is structurally similar to the bacillus cereus alkyl base dna glycosylase (alkd), was identified. this determinant was involved in the transcriptional repression of flagellar motility genes and was named adlp ... | 2016 | 27316964 |
| thusin, a novel two-component lantibiotic with potent antimicrobial activity against several gram-positive pathogens. | due to the rapidly increasing prevalence of multidrug-resistant bacterial strains, the need for new antimicrobial drugs to treat infections has become urgent. bacteriocins, which are antimicrobial peptides of bacterial origin, are considered potential alternatives to conventional antibiotics and have attracted widespread attention in recent years. among these bacteriocins, lantibiotics, especially two-component lantibiotics, exhibit potent antimicrobial activity against some clinically relevant ... | 2016 | 27486447 |
| bacillus cereus ar156-induced resistance to colletotrichum acutatum is associated with priming of defense responses in loquat fruit. | the effectiveness of a biocontrol agent bacillus cereus ar156 for control of anthracnose rot caused by colletotrichum acutatum in harvested loquat fruit and the possible mechanisms of its action have been investigated. treatment of fruit with b. cereus ar156 resulted in lower disease incidence and smaller lesion diameters compared with that of untreated fruit. the treatment enhanced activities of defense-related enzymes including chitinase, β-1, 3-glucanase, phenylalanine ammonia-lyase, peroxida ... | 2014 | 25386680 |
| use of ferrous iron by metallo-β-lactamases. | metallo-β-lactamases (mbls) catalyse the hydrolysis of almost all β-lactam antibacterials including the latest generation carbapenems and are a growing worldwide clinical problem. it is proposed that mbls employ one or two zinc ion cofactors in vivo. isolated mbls are reported to use transition metal ions other than zinc, including copper, cadmium and manganese, with iron ions being a notable exception. we report kinetic and biophysical studies with the di-iron(ii)-substituted metallo-β-lactamas ... | 2016 | 27498591 |
| the denitrification characteristics of pseudomonas stutzeri sc221-m and its application to water quality control in grass carp aquaculture. | to reduce ammonium and nitrite in aquaculture water, an isolate of the denitrifying bacterium pseudomonas stutzeri, sc221-m, was obtained. the effects of various nitrogen and carbon sources, the ratio of carbon to nitrogen and temperature on bacterial growth, denitrification rates and the expression levels of nirs and nosz in sc221-m were studied. the following conditions were determined to be optimal for growth and denitrification in sc221-m: nano2 as the nitrogen source, sodium citrate as the ... | 2014 | 25489740 |
| evaluation of a parchment document, the 13th century incorporation charter for the city of krakow, poland, for microbial hazards. | the literature of environmental microbiology broadly discusses issues associated with microbial hazards in archives, but these publications are mainly devoted to paper documents. there are few articles on historical parchment documents, which used to be very important for the development of literature and the art of writing. these studies present a broad spectrum of methods for the assessment of biodeterioration hazards of the parchment document in question. they are based on both conventional m ... | 2016 | 26896133 |
| oligo-dna custom macroarray for monitoring major pathogenic and non-pathogenic fungi and bacteria in the phyllosphere of apple trees. | to monitor the richness in microbial inhabitants in the phyllosphere of apple trees cultivated under various cultural and environmental conditions, we developed an oligo-dna macroarray for major pathogenic and non-pathogenic fungi and bacteria inhabiting the phyllosphere of apple trees. | 2012 | 22479577 |
| ces locus embedded proteins control the non-ribosomal synthesis of the cereulide toxin in emetic bacillus cereus on multiple levels. | the emetic toxin cereulide produced by bacillus cereus is synthesized by the modular enzyme complex ces that is encoded on a pxo1-like megaplasmid. to decipher the role of the genes adjacent to the structural genes cesa/cesb, coding for the non-ribosomal peptide synthetase (nrps), gene inactivation- and overexpression mutants of the emetic strain f4810/72 were constructed and their impact on cereulide biosynthesis was assessed. the hydrolase cesh turned out to be a part of the complex regulatory ... | 2015 | 26528255 |
| bacillus cereus efflux protein bc3310 - a multidrug transporter of the unknown major facilitator family, umf-2. | phylogenetic classification divides the major facilitator superfamily (mfs) into 82 families, including 25 families that are comprised of transporters with no characterized functions. this study describes functional data for bc3310 from bacillus cereus atcc 14579, a member of the "unknown major facilitator family-2" (umf-2). bc3310 was shown to be a multidrug efflux pump conferring resistance to ethidium bromide, sds and silver nitrate when heterologously expressed in escherichia coli dh5α δacrab. ... | 2015 | 26528249 |
| isolation, identification and characterization of paenibacillus polymyxa cr1 with potentials for biopesticide, biofertilization, biomass degradation and biofuel production. | paenibacillus polymyxa is a plant-growth promoting rhizobacterium that could be exploited as an environmentally friendlier alternative to chemical fertilizers and pesticides. various strains have been isolated that can benefit agriculture through antimicrobial activity, nitrogen fixation, phosphate solubilization, plant hormone production, or lignocellulose degradation. however, no single strain has yet been identified in which all of these advantageous traits have been confirmed. | 2016 | 27756215 |
| bacillus cereus ar156 primes induced systemic resistance by suppressing mir825/825* and activating defense-related genes in arabidopsis. | small rnas play an important role in plant immune responses. however, their regulatory function in induced systemic resistance (isr) is nascent. bacillus cereus ar156 is a plant growth-promoting rhizobacterium that induces isr in arabidopsis against bacterial infection. here, by comparing small rna profiles of pseudomonas syringae pv. tomato (pst) dc3000-infected arabidopsis with and without ar156 pretreatment, we identified a group of arabidopsis micrornas (mirnas) that are differentially regul ... | 2016 | 26526683 |
| isolation and characterization of a bacteriophage preying an antifungal bacterium. | several bacillus species were isolated from rice field soils, and 16s rrna gene sequence analysis showed that bacillus cereus was the most abundant. a strain named bc1 showed antifungal activity against rhizoctonia solani. bacteriophages infecting strain bc1 were isolated from the same soil sample. the isolated phage pk16 had an icosahedral head of 100 ± 5 nm and tail of 200 ± 5 nm, indicating that it belonged to the family myoviridae. analysis of the complete linear dsdna genome revealed a 158, ... | 2016 | 27904467 |
| antimicrobial constituents of the leaves of mikania micrantha h. b. k. | to isolate plant-derived compounds with antimicrobial activity from the leaves of mikania micrantha, to determine the compounds configuration, and to evaluate their antimicrobial activity against eight plant pathogenic fungi (exserohilum turcicum, colletotrichum lagenarium, pseudoperonispora cubensis, botrytis cirerea, rhizoctonia solani, phytophthora parasitica, fusarium solani, and pythium aphanidermatum,) and four plant pathogenic bacteria (gram negative bacteria: ralstonia dolaanacearum, xan ... | 2013 | 24098556 |
| diffusible signal factor (dsf) quorum sensing signal and structurally related molecules enhance the antimicrobial efficacy of antibiotics against some bacterial pathogens. | extensive use of antibiotics has fostered the emergence of superbugs that are resistant to multidrugs, which becomes a great healthcare and public concern. previous studies showed that quorum sensing signal dsf (diffusible signal factor) not only modulates bacterial antibiotic resistance through intraspecies signaling, but also affects bacterial antibiotic tolerance through interspecies communication. these findings motivate us to exploit the possibility of using dsf and its structurally related ... | 2014 | 24575808 |
| antimicrobial and genotoxicity effects of zero-valent iron nanoparticles. | in a world of nanotechnology, the first concern is the potential environmental impact of nanoparticles. an efficient way to estimate nanotoxicity is to monitor the responses of bacteria exposed to these particles. | 2014 | 25147712 |
| identification and characterization of the endophytic plant growth prompter bacillus cereus strain mq23 isolated from sophora alopecuroides root nodules. | endophytes mq23 and mq23r isolated from sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. results showed that mq23 and mq23r are potential n2-fixing endophytes and belong to the same species as bacillus cereus. mq23 was shown to be able to produce siderophores, iaa, and demonstrate certain antifungal activity to plant pathogenic fungi. co-inoculation with mq23+mq23ii showed a more significant effe ... | 2011 | 24031669 |
| tuning of thioredoxin redox properties by intramolecular hydrogen bonds. | thioredoxin-like proteins contain a characteristic c-x-x-c active site motif and are involved in a large number of biological processes ranging from electron transfer, cellular redox level maintenance, and regulation of cellular processes. the mechanism for deprotonation of the buried c-terminal active site cysteine in thioredoxin, necessary for dissociation of the mixed-disulfide intermediate that occurs under thiol/disulfide mediated electron transfer, is not well understood for all thioredoxi ... | 2013 | 23936007 |
| molecular typing in public health laboratories: from an academic indulgence to an infection control imperative. | using three austrian case studies, the variegated applications of molecular typing in today's public health laboratories are discussed to help illustrate preventive management strategies relying on dna subtyping. dna macrorestriction analysis by pulsed field gel electrophoresis has become the gold standard for subtyping of food borne pathogens like listeria, salmonella, campylobacter and bacillus cereus. using a salmonella mbandaka outbreak from the year 2010 as example, it is shown how the comp ... | 2012 | 22389752 |
| melanin-like pigment synthesis by soil bacillus weihenstephanensis isolates from northeastern poland. | although melanin is known for protecting living organisms from harmful physical and chemical factors, its synthesis is rarely observed among endospore-forming bacillus cereus sensu lato. here, for the first time, we reported that psychrotolerant bacillus weihenstephanensis from northeastern poland can produce melanin-like pigment. we assessed physicochemical properties of the pigment and the mechanism of its synthesis in relation to b. weihenstephanensis genotypic and phenotypic characteristics. ... | 2015 | 25909751 |
| screening of biological activities of polygonum maritimum l. from algerian coast. | to investigate the antioxidant and the antibacterial activities of crude extract from aerial part of polygonum maritimum l. (polygonaceae) (p. maritimum) and to find new actives biomolecules. | 2013 | 23905017 |
| extended genetic analysis of brazilian isolates of bacillus cereus and bacillus thuringiensis. | multiple locus sequence typing (mlst) was undertaken to extend the genetic characterization of 29 isolates of bacillus cereus and bacillus thuringiensis previously characterized in terms of presence/absence of sequences encoding virulence factors and via variable number tandem repeat (vntr). additional analysis involved polymerase chain reaction for the presence of sequences (be, cytk, ina, pag, lef, cya and cap), encoding putative virulence factors, not investigated in the earlier study. mlst a ... | 2013 | 23440117 |
| endophthalmitis. | endophthalmitis means bacterial or fungal infection inside the eye involving the vitreous and/or aqueous humors. most cases are exogenous and occur after eye surgery, after penetrating ocular trauma, or as an extension of corneal infection. an increasing number of cases are occurring after intravitreal injections of anti-vascular endothelial growth factor (vegf) medications. endophthalmitis may also be endogenous, arising from bacteraemic or fungaemic seeding of the eye. the infected eye never s ... | 2013 | 23438028 |
| isopeptide bonds of the major pilin protein bcpa influence pilus structure and bundle formation on the surface of bacillus cereus. | bacillus cereus strains elaborate pili on their surface using a mechanism of sortase-mediated cross-linking of major and minor pilus components. here we used a combination of electron microscopy and atomic force microscopy to visualize these structures. pili occur as single, double or higher order assemblies of filaments formed from monomers of the major pilin, bcpa, capped by the minor pilin, bcpb. previous studies demonstrated that within assembled pili, four domains of bcpa - cna(1), cna(2), ... | 2012 | 22624947 |
| antibacterial activity of 2-alkynoic fatty acids against multidrug-resistant bacteria. | the first study aimed at determining the structural characteristics needed to prepare antibacterial 2-alkynoic fatty acids (2-afas) was accomplished by synthesizing several 2-afas and other analogs in 18-76% overall yields. among all the compounds tested, the 2-hexadecynoic acid (2-hda) displayed the best overall antibacterial activity against gram-positive staphylococcus aureus (mic=15.6 μg/ml), staphylococcus saprophyticus (mic=15.5 μg/ml), and bacillus cereus (mic=31.3 μg/ml), as well as agai ... | 2013 | 24365283 |
| pcr detection and identification of bacterial contaminants in ocular samples from post-operative endophthalmitis. | bacterial endophthalmitis is a sight-threatening complication of ocular surgery which requires urgent medical consideration including comprehensive diagnosis. polymerase chain reaction (pcr) as a sensitive molecular method has been extensively used for detection of microbial species in clinical specimens. | 2015 | 26023576 |
| genomic characterization of the bacillus cereus sensu lato species: backdrop to the evolution of bacillus anthracis. | the key genes required for bacillus anthracis to cause anthrax have been acquired recently by horizontal gene transfer. to understand the genetic background for the evolution of b. anthracis virulence, we obtained high-redundancy genome sequences of 45 strains of the bacillus cereus sensu lato (s.l.) species that were chosen for their genetic diversity within the species based on the existing multilocus sequence typing scheme. from the resulting data, we called more than 324,000 new genes repres ... | 2012 | 22645259 |
| iron acquisition in bacillus cereus: the roles of ilsa and bacillibactin in exogenous ferritin iron mobilization. | in host-pathogen interactions, the struggle for iron may have major consequences on the outcome of the disease. to overcome the low solubility and bio-availability of iron, bacteria have evolved multiple systems to acquire iron from various sources such as heme, hemoglobin and ferritin. the molecular basis of iron acquisition from heme and hemoglobin have been extensively studied; however, very little is known about iron acquisition from host ferritin, a 24-mer nanocage protein able to store tho ... | 2014 | 24550730 |
| high antioxidant and dna protection activities of n-acetylglucosamine (glcnac) and chitobiose produced by exolytic chitinase from bacillus cereus ew5. | chitin-degrading bacterial strains were screened and tested for their ability to degrade shrimp-shell waste (ssw). among the potential strains, b. cereus ew5 exhibited the highest chitin-degrading ability compared with other strains and produced 24 mg of reducing sugar per gram of dry ssw after 4 days of incubation. a tlc analysis of ssw biodegradation revealed that the chitosaccharides produced in the culture supernatant were mainly n-acetylglucosamine (glcnac) and chitobiose due to the isolate ... | 2014 | 25077065 |
| in vitro evaluation of antimicrobial efficacy of extracts obtained from raw and fermented wild macrofungus, lenzites quercina. | in recent time, there is a major concern about antibiotic resistance displayed by some pathogenic microorganisms and this had involved a continuous search for natural antimicrobial products. the phytochemistry as well as antimicrobial activity of extracts obtained from lenzites quercina was investigated. the extracts and purified fractions were, respectively, tested against indicator organisms using agar well diffusion and disc diffusion methods. the quantity of phytochemicals found in the extra ... | 2015 | 26604928 |
| phylogenetic relationship of phosphate solubilizing bacteria according to 16s rrna genes. | phosphate solubilizing bacteria (psb) can convert insoluble form of phosphorous to an available form. applications of psb as inoculants increase the phosphorus uptake by plant in the field. in this study, isolation and precise identification of psb were carried out in malaysian (serdang) oil palm field (university putra malaysia). identification and phylogenetic analysis of 8 better isolates were carried out by 16s rrna gene sequencing in which as a result five isolates belong to the beta subdiv ... | 2015 | 25632387 |
| medicinal attributes of major phenylpropanoids present in cinnamon. | excessive production of free radicals has been implicated in many diseases including cancer. they are highly reactive and bring about oxidation of biomolecules i.e., proteins, lipids and nucleic acids which are associated with many degenerative diseases. natural products acting as antioxidants have ability to neutralize free radicals and their actions and hence they mitigate their harmful effects. the present study was designed to investigate pharmacological properties viz., antioxidant, antibac ... | 2016 | 27245453 |
| clostridium and bacillus binary enterotoxins: bad for the bowels, and eukaryotic being. | some pathogenic spore-forming bacilli employ a binary protein mechanism for intoxicating the intestinal tracts of insects, animals, and humans. these gram-positive bacteria and their toxins include clostridium botulinum (c2 toxin), clostridium difficile (c. difficile toxin or cdt), clostridium perfringens (ι-toxin and binary enterotoxin, or bec), clostridium spiroforme (c. spiroforme toxin or cst), as well as bacillus cereus (vegetative insecticidal protein or vip). these gut-acting proteins for ... | 2014 | 25198129 |
| biodegradability of commercial and weathered diesel oils. | this work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 -dichlorophenol indophenol (dcpip) and soil respirometric experiments using biometer flasks. in the former we tested the bacterial cultures staphylococcus hominis, kocuria palustris, ... | 2008 | 24031193 |
| improving glyphosate oxidation activity of glycine oxidase from bacillus cereus by directed evolution. | glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (go) has been reported to be able to catalyze the oxidative deamination of various amines and cleave the c-n bond in glyphosate. here, in an effort to improve the catalytic activity of the glycine oxidase that was cloned from a glyphosate-degrading marine strain of bacillus cereus (bcego), we used a bacteriophage ... | 2013 | 24223901 |
| bacterial quorum sensing: its role in virulence and possibilities for its control. | quorum sensing is a process of cell-cell communication that allows bacteria to share information about cell density and adjust gene expression accordingly. this process enables bacteria to express energetically expensive processes as a collective only when the impact of those processes on the environment or on a host will be maximized. among the many traits controlled by quorum sensing is the expression of virulence factors by pathogenic bacteria. here we review the quorum-sensing circuits of st ... | 2012 | 23125205 |
| antimicrobial and anti-quorum sensing activities of selected medicinal plants of ethiopia: implication for development of potent antimicrobial agents. | traditional medicinal plants have been used as an alternative medicine in many parts of the world, including ethiopia. there are many documented scientific reports on antimicrobial activities of the same. to our knowledge, however, there is no report on the anti-quorum sensing (quorum quenching, qq) potential of traditional ethiopian medicinal plants. as many of the opportunistic pathogenic bacteria depend on quorum sensing (qs) systems to coordinate their virulence expression, interference with ... | 2016 | 27400878 |
| vector potential of blattella germanica (l.) (dictyoptera: blattidae) for medically important bacteria at food handling establishments in jimma town, southwest ethiopia. | cockroaches have been regarded as possible vectors of human enteropathogens. their presence and crawl particularly in food handling establishments could be risky for human health. therefore, this study was done to determine the vector potential of cockroach for medically important bacterial pathogens in restaurants and cafeterias. a cross-sectional study was conducted on cockroaches from restaurants and cafeterias in jimma town from may to september 2014. standard taxonomic keys and microbiologi ... | 2016 | 27294115 |
| hygienic-sanitary evaluation of sushi and sashimi sold in messina and catania, italy. | sushi and sashimi are traditional japanese food, mostly consisting of raw seafood alone or in combination with rice. eating sushi and sashimi has become popular in many countries even outside japan. this food is not free from health risks such as ingestion of pathogenic bacteria or parasite. the aim of this study was to investigate on hygienic-sanitary quality of sushi and sashimi sold in the cities of messina and catania, southern italy. fifty samples (38 sushi and 12 sashimi) were analysed to ... | 2014 | 27800343 |
| development of a novel selective and differential medium for the isolation of listeria monocytogenes. | a new medium (lecithin and levofloxacin [ll] medium) is described for the isolation of listeria monocytogenes from food samples. ll medium includes lecithin from soybeans for the detection of phosphatidylinositol-specific phospholipase c (pi-plc) and phosphatidylcholine-specific phospholipase c (pc-plc) produced by l. monocytogenes. levofloxacin is incorporated to inhibit the growth of microorganisms other than l. monocytogenes, especially bacillus cereus, shown to possess pi-plc and pc-plc acti ... | 2014 | 24271177 |
| structural basis for latency and function of immune inhibitor a metallopeptidase, a modulator of the bacillus anthracis secretome. | immune inhibitor a(inha)-type metallopeptidases are potential virulence factors secreted by members of the bacillus cereus group. two paralogs from anthrax-causing bacillus anthracis (bainha1 and bainha2) were shown to degrade host tissue proteins with broad substrate specificity. analysis of their activation mechanism and the crystal structure of a zymogenic bainha2 variant revealed a ∼750-residue four-domain structure featuring a pro-peptide, a catalytic domain, a domain reminiscent of viral e ... | 2016 | 26745529 |
| detection of carboxylesterase and esterase activity in culturable gut bacterial flora isolated from diamondback moth, plutella xylostella (linnaeus), from india and its possible role in indoxacarb degradation. | diamondback moth (dbm), plutella xylostella (linnaeus), is a notorious pest of brassica crops worldwide and is resistant to all groups of insecticides. the insect system harbors diverse groups of microbiota, which in turn helps in enzymatic degradation of xenobiotic-like insecticides. the present study aimed to determine the diversity of gut microflora in dbm, quantify esterase activity and elucidate their possible role in degradation of indoxacarb. we screened 11 geographic populations of dbm i ... | 2016 | 26991291 |
| antimicrobial activity, growth inhibition of human tumour cell lines, and phytochemical characterization of the hydromethanolic extract obtained from sapindus saponaria l. aerial parts. | the hydromethanolic extract of sapindus saponaria l. aerial parts was investigated for antimicrobial activity (against several gram-positive and gram-negative bacteria and fungi) and capacity to inhibit the growth of different human tumor cell lines as also nontumor liver cells. the evaluated extract was further characterized in terms of phytochemicals using uv, (1)h-nmr, (13)c-nmr, and ms spectroscopic tools. the extract has shown a significant antimicrobial activity on all tested bacterial and ... | 2013 | 24455713 |
| brown algae (phaeophyceae) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products. | eight species of brown algae (phaeophyceae) from the coast of madagascar have been investigated for their chemical constituents. fucosterol (3) was obtained as the most abundant compound. the brown alga sargassum ilicifolium was the source for the first isolation of the terpenoid c27-alcohol 1,1',2-trinorsqualenol (1) from marine sources. from s. incisifolium we isolated the highly unsaturated glycolipid 1-o-palmitoyl-2-o-stearidonoyl-3-o-β-d-galactopyranosylglycerol (4) and we report the first ... | 2015 | 26358714 |
| structural characterization and antimicrobial activity of a biosurfactant obtained from bacillus pumilus dsvp18 grown on potato peels. | biosurfactants constitute a structurally diverse group of surface-active compounds derived from microorganisms. they are widely used industrially in various industrial applications such as pharmaceutical and environmental sectors. major limiting factor in biosurfactant production is their production cost. | 2015 | 26495106 |
| red algae (rhodophyta) from the coast of madagascar: preliminary bioactivity studies and isolation of natural products. | several species of red algae (rhodophyta) from the coastal regions of madagascar have been investigated for their natural products. the most abundant compound was cholesterol (5) in combination with a series of oxidized congeners. the brominated indoles 1-3 along with the sesquiterpene debilone (4) have been isolated from laurencia complanata. for the first time, debilone (4) has been obtained from a marine plant. from the methanol extract of calloseris sp., we have achieved the second isolation ... | 2015 | 26198236 |
| in vitro characterization of a recombinant ahl-lactonase from bacillus cereus isolated from a striped catfish (pangasianodon hypophthalmus) pond. | aiia gene encoding ahl-lactonase was isolated from bacillus cereus strain n26.2, originating from a striped catfish (pangasianodon hypophthalmus) pond in vietnam. this gene, abbreviated as aiia(n26.2), was cloned and expressed in a competent escherichia coli strain bl21(de3)plyss. the resulting protein, abbreviated as aiian26.2, was highly active in the ph range of 6-8 and could retain 80 % of the maximum activity under storage for 5 days at 4 °c or for 3 days at 20 °c. these properties of aiian ... | 2013 | 24426155 |
| studies on antimicrobial and immunomodulatory effects of hot aqueous extract of acacia nilotica l. leaves against common veterinary pathogens. | acacia nilotica is a plant species that is almost ubiquitously found in different parts of the world. various preparations of it have been advocated in folk medicine for the treatment of tuberculosis, leprosy, smallpox, dysentery, cough, ophthalmia, toothache, skin cancer as astringent, antispasmodic, and aphrodisiac since immemorial times. the present study investigates the antibacterial, antifungal, antiviral, and immunomodulatory potential of hot aqueous extract (hae) of acacia nilotica leave ... | 2014 | 24804150 |
| mosquitocidal properties of bacillus species isolated from mangroves of vellar estuary, southeast coast of india. | samples collected from the mangroves of vellar estuary yielded a mosquitocidal bacterium, whose secondary metabolites exhibited mosquito larvicidal and pupicidal activity. the bacterium was isolated using standard microbiological methods and identified using classical biochemical tests. the mosquitocidal bacterium was identified as bacillus subtilis, bacillus thuringiensis, bacillus sphaericus and bacillus cereus. mosquitocidal metabolite(s) was separated from the culture supernatant of the bact ... | 2013 | 26345039 |
| role of sphingomyelinase in infectious diseases caused by bacillus cereus. | bacillus cereus (b. cereus) is a pathogen in opportunistic infections. here we show that bacillus cereus sphingomyelinase (bc-smase) is a virulence factor for septicemia. clinical isolates produced large amounts of bc-smase, grew in vivo, and caused death among mice, but atcc strains isolated from soil did not. a transformant of the atcc strain carrying a recombinant plasmid containing the bc-smase gene grew in vivo, but that with the gene for e53a, which has little enzymatic activity, did not. ... | 2012 | 22701599 |
| draft genome sequences of two bacillus thuringiensis strains and characterization of a putative 41.9-kda insecticidal toxin. | in this work, we report the genome sequencing of two bacillus thuringiensis strains using illumina next-generation sequencing technology (ngs). strain hu4-2, toxic to many lepidopteran pest species and to some mosquitoes, encoded genes for two insecticidal crystal (cry) proteins, cry1ia and cry9ea, and a vegetative insecticidal protein (vip) gene, vip3ca2. strain leapi01 contained genes coding for seven cry proteins (cry1aa, cry1ca, cry1da, cry2ab, cry9ea and two cry1ia gene variants) and a vip3 ... | 2014 | 24784323 |
| characterization of a novel manduca sexta beta-1, 3-glucan recognition protein (βgrp3) with multiple functions. | recognition of pathogens by insect pattern recognition receptors is critical to mount effective immune responses. in this study, we reported a new member (βgrp3) of the β-1, 3-glucan recognition protein (βgrp) family from the tobacco hornworm manduca sexta. unlike other members of the m. sexta βgrp family proteins, which contain an n-terminal small glucan binding domain and a c-terminal large glucanase-like domain, βgrp3 is 40-45 residues shorter at the n-terminus and lacks the small glucan bind ... | 2014 | 24952171 |