| expressed sequences and polymorphisms in rohu carp (labeo rohita, hamilton) revealed by mrna-seq. | expressed genes and polymorphisms were identified in lines of rohu labeo rohita selected for resistance or susceptibility to aeromonas hydrophila, an important bacterial pathogen causing aeromoniasis. all animals were grown in a common environment and rna from ten individuals from each line pooled for illumina mrna-seq. de novo transcriptome assembly produced 137,629 contigs with 40× average coverage.forty-four percent of the assembled sequences were annotated with gene names and ontology terms. ... | 2012 | 22298294 |
| interactions of ocular isolates of gram-negative bacteria with lactoferrin. | the aim was to examine the interaction of lactoferrin with gram-negative bacteria isolated from ocular adverse responses. | 2012 | 22293406 |
| the first chordate big defensin: identification, expression and bioactivity. | defensins are broadly present in plants, invertebrates and vertebrates, but little information is available about it in amphioxus, a protochordate holding a key phylogenetic position. in this study, a big defensin cdna was identified from the amphioxus branchiostoma japonicum (termed bjbd). the cdna contained an open reading frame (orf) of 354 bp encoding a 117 amino acid protein, which had an n-terminal signal sequence followed by a propeptide and the mature big defensin. the mature peptide had ... | 2012 | 22281606 |
| fulminant aeromonas hydrophila infection during acute lymphoblastic leukemia treatment. | aeromonas hydrophila septicemia has a fulminant course and it has been usually reported in immunocompromised hosts and rarely among children with leukemia. high morbidity and mortality is associated with a hydrophila infections. we describe the case of a child with acute lymphoblastic leukemia who presented with septicemia due to a hydrophila. the patient presented with fever and skin discoloration during a febrile neutropenia episode, which rapidly evolved into bacteremia and extensive thigh su ... | 2012 | 22265850 |
| thin-film fixed-bed reactor (tffbr) for solar photocatalytic inactivation of aquaculture pathogen aeromonas hydrophila. | outbreaks of infectious diseases by microbial pathogens can cause substantial losses of stock in aquaculture systems. there are several ways to eliminate these pathogens including the use of antibiotics, biocides and conventional disinfectants, but these leave undesirable chemical residues. conversely, using sunlight for disinfection has the advantage of leaving no chemical residue and is particularly suited to countries with sunny climates. titanium dioxide (tio2) is a photocatalyst that increa ... | 2012 | 22243515 |
| inability of some aeromonas hydrophila strains to act as recipients of plasmid pras1 in conjugal transfer experiments. | plasmids belonging to the incu incompatibility group are mobile genetic elements isolated frequently from aeromonas spp. these plasmids share structural and functional characteristics and often carry class-1 integrons bearing antibiotic resistance genes. in this work the ability of two incu plasmids, par-32 and pras1 to establish in different a. hydrophila strains after conjugal transfer was studied. in vitro transfer frequencies on solid surface ranged from 10(-1) to 10(-6) for par-32 and from ... | 2012 | 22228423 |
| potential application of extracts from indian almond (terminalia catappa linn.) leaves in siamese fighting fish (betta splendens regan) culture. | indian almond (terminalia catappa linn.) leaves with green and red coloration were tested for bactericidal activity with pathogenic bacteria and their acute toxicity to siamese fighting fish (betta splendens regan). powder of indian almond leaves was extracted with water at the ratio of 1:10 (w/v) and then freeze-dried to a dry powder. bactericidal efficacy was tested against 28 isolates of pathogenic bacteria (aeromonas hydrophila, edwardsiella tarda, enterobacter spp., plesiomonas shigelloides ... | 2012 | 23885412 |
| clinical and epidemiologic features of diarrheal disease due to aeromonas hydrophila and plesiomonas shigelloides infections compared with those due to vibrio cholerae non-o1 and vibrio parahaemolyticus in bangladesh. | using data from the international centre for diarrhoeal disease research, bangladesh (icddr,b) from 1996 to 2001, we compared the clinical features of diarrhea in patients with stool specimens yielding only a. hydrophila (189 patients; 1.4% of 13,970 patients screened) or p. shigelloides (253 patients) compared to patients with sole v. cholerae non-o1 infection (99 patients) or v. parahaemolyticus infection (126 patients). patients exhibited similar frequencies of fever (temperature >37.8°c), st ... | 2012 | 23762755 |
| fatal septicemia and endotoxic shock due to aeromonas hydrophila. | although rare, bloodstream infections caused by aeromonas tend to be very severe and progress rapidly. | 2012 | 23569493 |
| antagonistic activity of cellular components of potential probiotic bacteria, isolated from the gut of labeo rohita, against aeromonas hydrophila. | the objective of this study was to characterise the antagonistic activity of cellular components of potential probiotic bacteria isolated from the gut of healthy rohu (labeo rohita), a tropical freshwater fish, against the fish pathogen, aeromonas hydrophila. three potential probiotic strains (referred to as r1, r2, and r5) were screened using a well diffusion, and their antagonistic activity against a. hydrophila was determined. biochemical tests and 16s rrna gene analysis confirmed that r1, r2 ... | 2011 | 26781682 |
| the function of lactic acid bacteria and brine solutions on biogenic amine formation by foodborne pathogens in trout fillets. | the influences of lactic acid bacteria and brine solutions on the biogenic amine formation by staphylococcus aureus, escherichia coli, klebsiella pneumoniae, enterococus faecalis, pseudomonas aeruginosa, listeria monocytogenes, aeromonas hydrophila and salmonella paratyphi a in fermented trout fillets were investigated. fish fillets were divided into four groups, group 1 without any lactic acid bacteria inoculation, group 2 and group 3 with different salt concentration inoculated with lactic aci ... | 2011 | 25212358 |
| synergistic and antagonistic effect of lactic acid bacteria on tyramine production by food-borne pathogenic bacteria in tyrosine decarboxylase broth. | the effect of lactic acid bacteria (lab) strains on tyramine (tyr) and also other biogenic amines (ba) production by eight common food-borne pathogen (fbp) in tyrosine decarboxylase broth (tdb) was investigated by using a rapid hplc method. significant differences were observed among the fbp strains in ammonia (amn) and ba production apart from tryptamine, histamine (his) and spermine formation (p<0.05). salmonella paratyphi a was characterised as the main amine producer. lab had an important sy ... | 2011 | 25214109 |
| in vitro antibacterial activities of ethanol extract of iranian propolis (eeip) against fish pathogenic bacteria (aeromonas hydrophila, yersinia ruckeri & streptococcus iniae). | the "in vitro" antibacterial activity of ethanol extract of propolis (eeip) from urmia, iran was investigated against three prevalent species of fish bacterial pathogens including: aeromonas hydrophila lmg 3770, yersinia ruckeri lmg 3279 and streptococcus iniae lmg 14520. in this study two standard susceptibility testing techniques (micro-broth dilution method and agar-well diffusion method) were used to evaluation of the antibacterial activity of eeip against the mentioned micro-organisms. also ... | 2010 | 24031591 |
| virulence and cytotoxicity of seafood borne aeromonas hydrophila. | the present study was conducted to determine the virulence and cytotoxicity of aeromonas hydrophila strains isolated from seafood samples collected from 5 major fish markets in chennai, tamil nadu, india. among 73 a. hydrophila strains isolated from fish and shrimp samples, 86.3% exhibited haemolysis, 78.1% produced slime, 98.63% produced protease and also demonstrated cytotoxicity on vero cells. cell shrinkage, detachment and rounding of vero cells were recorded as cytotoxic changes. only one s ... | 2010 | 24031577 |
| bioactive potential of streptomyces against fish and shellfish pathogens. | in the present study, isolation of streptomyces associated with marine sponges and its bioactive potential against fish and shellfish pathogens were assessed. the streptomyces sp. were isolated from the marine sponges namely callyspongia diffusa, mycale mytilorum, tedania anhelans and dysidea fragilis collected from vizhinjam port, situated in the south-west coast of india. | 2010 | 22347566 |
| presence of bla tem-116 gene in environmental isolates of aeromonas hydrophila and aeromonas jandaei from brazil. | it is known that aeromonas spp. possess different chromosomal β-lactamase genes. presence and phenotypic expression of bla tem, bla shv, and bla ctx-m esbl-encoding genes were investigated in environmental water isolates of aeromonas hydrophila and aeromonas jandaei. presence of bla shv and bla ctx-m genes was not observed, and bla tem gene was verified in 91% of the isolates. sequencing of 10 fragments showed the occurrence of bla tem-116. | 2010 | 24031548 |
| detection of toxigenic strains of aeromonas species in foods by a multiplex pcr assay. | aeromonas hydrophila and other aeromonads are ubiquitous organisms found in meat, vegetables, drinking water and various other food items. they cause diarrhea and extra-intestinal infections in normal and immunocompromised patients. the aim of the study was to develop a multiplex pcr assay for the detection of virulence-associated genes of aeromonas including hemolysin (hlya), aerolysin (aera), glycerophospholipid-cholesterol acyl transferase (gcat) alongwith a 16s rrna gene. internal amplificat ... | 2010 | 23100820 |
| necrotizing fasciitis caused by inconspicuous infection of aeromonas hydrophila in an immunocompromised host. | we present a case of serious necrotizing fasciitis due to aeromonas hydrophila without a suggestive history of routes of pathogen invasion in a 60-year-old male. despite prompt broad-spectrum antibiotic and extensive surgical therapies, the patient died within 72 hours following initial presentation. our experience suggests clinicians be highly alert to the disease when an immunocompromised patient featured fulminant soft-tissue infection in the endemic area. | 2010 | 24946336 |
| aeromonas hydrophila sepsis with septic embolism and rhabdomyolysis in a chronic iron overload haemodialysis patient treated with deferoxamine. | aeromonas infection in humans is associated with certain underlying diseases, especially chronic liver disease or malignancy. however, aeromonas infection associated with iron overload is rarely reported. we report a case of a 47-year-old female with end-stage renal disease on haemodialysis and on deferoxamine treatment for iron overload who developed aeromonas sepsis with septic embolism and rhabdomyolysis. although the patients with aeromonas infection and rhabdomyolysis have been correlated w ... | 2009 | 25984022 |
| phylogenetic profiling of bacterial community from two intimately located sites in balramgari, north-east coast of india. | microbial communities in coastal subsurface sediments play an important role in biogeochemical cycles. in this study microbial communities in tidal subsurface sediments of balramgari in the state of orissa, india were investigated using a culture independent approach. two 16s rdna cloned libraries were prepared from the closely located (100 m along the coast) subsurface sediment samples. library i sediment samples had higher organic carbon content but lower sand percentage in comparison to libra ... | 2009 | 23100766 |
| detection of aerolysin gene in aeromonas hydrophila isolated from fish and pond water. | aerolysin is a hemolytic toxin encoded by aerolysin gene (1482 bp) that plays a key role in the pathogenesis of aeromonas hydrophila infection in fish. new speciesspecific primers were designed to amplify 326 bp conserved region of aerolysin gene for a. hydrophila. twenty-five isolates of a. hydrophila recovered from fish and pond water were studied for detection of aerolysin gene. aerolysin gene was detected in 85% of the isolates during the study. the designed primers were highly specific and ... | 2008 | 23100746 |
| antibacterial activity of plant extracts from brazil against fish pathogenic bacteria. | the aim of this work was to evaluate the antibacterial activity of brazilian plants extracts against fish pathogenic bacteria. forty six methanolic extracts were screened to identify their antibacterial properties against streptococcus agalactiae, flavobacterium columnare and aeromonas hydrophila. thirty one extracts showed antibacterial activity. | 2008 | 24031303 |
| necrotizing fasciitis and myonecrosis due to aeromonas hydrophila. | aeromonas hydrophila infection has been described as the cause of necrotizing fasciitis in patients with suppressed immune systems, burns, and trauma in an aquatic setting. the following presents a case in which severe necrotizing fasciitis and myonecrosis of the lower extremity, as well as toxic shock, developed as a result of wound infection from direct contact with brackish water. mixed aerobic and anaerobic bacteria, including aeromonas hydrophila, were isolated from the wound culture. this ... | 2007 | 26110366 |
| typing of aeromonas hydrophila of fish and human diarrhoeal origin by outer membrane proteins and lipopolysaccharides. | a study was undertaken to discriminate the strains of aeromonas hydrophila isolated from fish and diarrhoeal samples by sds-page analysis of outer membrane proteins (omps) and lipopolysaccharides (lpss). common bands at 47 kda positions for omps and at 31-38 kda for lpss were observed. no strain of a. hydrophila from clinical or fish samples was found identical in either omps or lpss profile. | 2007 | 23100639 |
| survival of aeromonas hydrophila and listeria monocytogenes on fresh vegetables stored under moderate vacuum. | storage at 6.5°c under moderate vacuum effectively prevented growth of aeromonas hydrophila on chicory endive, but had only a limited inhibitory effect on the growth of the organism on mung bean sprouts. growth of listeria monocytogenes on chicory endive was strongly stimulated under these conditions, whereas it was decreased on mung-bean sprouts. | 1994 | 24421192 |
| growth kinetics of aeromonas hydrophila in freshwaters supplemented with various organic and inorganic nutrients. | freshwaters of varying natural nutrient enrichment were used as growth media for the culture of an autochthonous, heterotrophic, freshwater bacterium, aeromonas hydrophila. the growth rate of the bacterium in eutrophic waters was increased to the greatest extent by adding carbon, as glucose; generation times decreased by up to 65%. additions of carbon and phosphorus increased the maximal cell densities by over 25-fold. in oligotrophic waters, bacterial growth was most strongly promoted by the si ... | 1994 | 24420938 |
| specific dna probes for the identification of the fish pathogen, renibacterium salmoninarum. | to obtain specific dna probes for the identification of the fish pathogen, renibacterium salmoninarum, a discriminatory recombinant dna library was constructed using selective fragments of the bacterial genome. three renibacterial clones, pmam29, pmam46 and pmam77, containing 149, 73, and 154 bp respectively, were isolated and characterized. the specificity of the probes was confirmed by dot-blot and southern hybridization analyses. bacterial hybridization experiments revealed that pmam29 discri ... | 1994 | 24420936 |
| characteristics of 0/129-sensitive motile aeromonas strains isolated from freshwater on starch-ampicillin agar. | motile aeromonas hydrophila strains were recovered from several freshwater sources by spread-plating water samples on starch-ampicillin agar, originally described as a medium for recovering aeromonas hydrophila quantitatively from foods. starch-ampicillin agar was compared with membrane aeromonas medium and rimler-shotts medium for selectivity for, and recovery of, aeromonas strains from freshwater. thirty-four aeromonas strains thus isolated were identified to species level by their phenotypic ... | 1992 | 24193138 |
| self-pelleting autoagglutination on mesophilic aeromonads belonging to serotype o: 11. | self-pelleting autoagglutination of aeromonas hydrophila and a. sobria strains from serogroup o:11 depends on the presence of the extracellular layer peripheral to the cell membrane (s layer) and motility. to show this phenotype, strains should not fimbriate during growth. | 1991 | 24424944 |
| the establishment of an intestinal microflora in developing goldfish (carassius auratus) of culture ponds. | the bacterial flora in the intestinal tract of goldfish (carassius auratus) was investigated at different stages of fish development. the floras of the diets and the water and sediment of a culture pond were also analyzed. the total counts in the intestine ranged from 2.2 × 10(6)-2.1 × 10(8) cells g(-1) wet weight.aeromonas hydrophila, a. punctata, pseudomonas, bacteroidaceae andclostridium species were the common components in the intestinal tract of goldfish from larvae to adult stage.bacteroi ... | 1988 | 24201410 |
| fate of bacteria ingested by larvae of the freshwater mayfly,ephemera danica. | the fate of bacteria in the food of a common freshwater invertebrate has been studied both in controlled laboratory experiments and in a stream sediment. the animal chosen was the larva of the burrowing mayfly,ephemera danica. it ingested all available bacteria nonselectively. more bacteria were found associated with the hindgut than with the mesenteron despite continuous plug flow of food through the alimentary canal. species of bacteria were affected in different ways.aeromonas hydrophila andc ... | 1988 | 24201409 |
| the preliminary assessment of anti-microbial activity of hplc separated components of kirkia wilmsii. | most communities in developing countries rely on traditional medicines for the treatment of diseases. in south africa, the limpopo province, within the lebowakgomo district, uses tuberous roots of kirkia wilmsii, after infusion in water for the treatment of a wide range of diseases by sotho communities. | 2014 | 25371593 |
| three stats are involved in the regulation of the expression of antimicrobial peptides in the triangle sail mussel, hyriopsis cumingii. | janus kinase (jak) and signal transducers and activators of transcription (stat) signaling pathway is associated in antiviral and antibacterial immune response. previous studies primarily investigated the function of stats in mammals. for most invertebrates, only one stat was found in each species, such as stat92e was found in drosophila melanogaster. the studies, which focus on the functional difference between various stats in the same species of invertebrate, are limited. in the present study ... | 2017 | 28214598 |
| role of bacillus licheniformis vs16-derived biosurfactant in mediating immune responses in carp rohu and its application to the food industry. | multifarious applications of bacillus licheniformis vs16-derived biosurfactant were explored. labeo rohita fingerlings were injected intraperitoneally with 0.1 ml of phosphate-buffered saline (pbs) containing purified biosurfactant at 0 (control), 55 (s55), 110 (s110), 220 (s220), or 330 (s330) μg ml(-1) concentrations. various immunological parameters and the expression of immune-related genes were measured at 7, 14, and 21 days post-administration (dpa). at 21 dpa, fish were challenged with ae ... | 2017 | 28400765 |
| a novel probiotic bacillus siamensis b44v isolated from thai pickled vegetables (phak-dong) for potential use as a feed supplement in aquaculture. | the use of probiotic bacteria to control bacterial infection in farmed fish is of clear practical interest. the aims of this study were to isolate and select a probiotic bacillus sp. and to evaluate the effects of its supplementation on the growth and disease resistance of hybrid catfish. bacillus siamensis strain b44v, selectively isolated from thai pickled vegetables (phak-dong), displayed a high potential as a probiotic in catfish culture. this bacterium produced a bacteriocin-like substance ... | 2017 | 28747586 |
| antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium bacillus subtilis mtcc 10403. | brown seaweed anthophycus longifolius (turner) kützing (family sargassaceae) associated heterotrophic bacterium bacillus subtilis mtcc 10403 was found to be a potent isolate with broad range of antibacterial activity against important perceptive food pathogens vibrio parahaemolyticus, v. vulnificus, and aeromonas hydrophila. this bacterium was positive for polyketide synthetase gene (kc589397), and therefore, was selected to bioprospect specialized metabolites bearing polyketide backbone. bioact ... | 2017 | 28704687 |
| a truncated sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in oryzias melastigma. | antimicrobial peptides (amps) represent an efficient part of innate immunity and are found in a variety of life. among them histone 2a (h2a), as a promising class of amps, attracts great attention, but the in vivo mechanism of h2a derived amp is still less known. based on the acquisition of sphistin, a synthetic 38-amino acid h2a derived peptide from scylla paramamosain, as reported in our previous study, was truncated into three short fragments (sph12-38, sph20-38 and sph30-38) and further inve ... | 2017 | 28600196 |
| effect of bacillus subtilis on aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp (ctenopharyngodon idella). | our study explored the effect of oral intubation of bacillus subtilis on aeromonas hydrophila-induced intestinal mucosal barrier function damage and inflammation in grass carp. the mid-intestine mucosal tissue was collected for atpase activity measurement. intestinal mucosa was also ultrastructurally examined with transmission electron microscope (tem), and its permeability was determined using evans blue (eb) and d-lactic acid. the mid-intestine pro-inflammation cytokine, myd88 and tight juncti ... | 2017 | 28484272 |
| seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium. | seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ... | 2017 | 28733150 |
| growth, blood health, antioxidant status, immune response and resistance to aeromonas hydrophila of juvenile yellow catfish exposed to di-2-ethylhexyl phthalate (dehp). | triplicate groups of juvenile yellow catfish pelteobagrus fulvidraco were exposed to three levels of dehp (0, 0.1 and 0.5mg l(-1)) for 56days. fish survival (100%) was not affected by different levels of ambient dehp. final body weight, weight gain, specific growth rate and feed intake of fish exposed to 0.5mg l(-1) dehp were the highest. on the contrary, hepatosomatic index of fish exposed to 0.1 and 0.5mg l(-1) dehp were the lowest. serum total protein, glutamic-pyruvic transaminase, glutamic- ... | 2017 | 28851534 |
| previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora. | a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ... | 2017 | 28842846 |
| modelling of microbial polyhydroxyalkanoate surface binding protein phap for rational mutagenesis. | phasins are unusual amphiphilic proteins that bind to microbial polyhydroxyalkanoate (pha) granules in nature and show great potential for various applications in biotechnology and medicine. despite their remarkable diversity, only the crystal structure of phapah from aeromonas hydrophila has been solved to date. based on the structure of phapah , homology models of phapaz from azotobacter sp. fa-8 and phaptd from halomonas bluephagenesis td were successfully established, allowing rational mutag ... | 2017 | 28840964 |
| fe(ii)/cu(ii) interaction on goethite stimulated by an iron-reducing bacteria aeromonas hydrophila hs01 under anaerobic conditions. | copper is a trace element essential for living creatures, but copper content in soil should be controlled, as it is toxic. the physical-chemical-biological features of cu in soil have a significant correlation with the fe(ii)/cu(ii) interaction in soil. of significant interest to the current study is the effect of fe(ii)/cu(ii) interaction conducted on goethite under anaerobic conditions stimulated by hs01 (a dissimilatory iron reduction (dir) microbial). the following four treatments were desig ... | 2017 | 28834771 |
| antibiotic and heavy metal resistance of aeromonas hydrophila and edwardsiella tarda isolated from red hybrid tilapia (oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis. | the aim of this study is to identify antibiogram and heavy metal resistance pattern of aeromonas hydrophila and edwardsiella tarda isolated from red hybrid tilapia (oreochromis spp.) coinfected with motile aeromonas septicemia and edwardsiellosis in four commercial fish farms. | 2017 | 28831226 |
| the expression of superoxide dismutase in mytilus coruscus under various stressors. | superoxide dismutases (sods), a by-product of antioxidative defence system, protects organisms for eliminating excess reactive oxygen species (ros) and maintaining the redox balance of immune system. the complete open reading frames (orfs) of cu/zn-sod and mn-sod were identified from mytilus coruscus (designated as mcsod and mnsod) by homologous cloning. the sequence lengths were 474bp and 687bp, encoding 157 and 228 amino acids respectively. the deduced amino acid sequences of mcsod and mnsod s ... | 2017 | 28823980 |
| threonine deficiency decreased intestinal immunity and aggravated inflammation associated with nf-κb and target of rapamycin signalling pathways in juvenile grass carp (ctenopharyngodon idella) after infection with aeromonas hydrophila. | this study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. six iso-nitrogenous semi-purified diets containing graded levels of threonine (3·99-21·66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged with aeromonas hydrophila for 14 d. results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozym ... | 2017 | 28820083 |
| in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus. | fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ... | 2017 | 28819794 |
| therapeutic effects of marshmallow (althaea officinalis l.) extract on plasma biochemical parameters of common carp infected with aeromonas hydrophila. | this study evaluated preclinical and clinical safety of marshmallow (althaea officinalis l.) extract as a naturopathic medicine in common carp deliberately infected with aeromonas hydrophila. the fish were fed 0 (control), 2.50, 5.00 and 10.00 g of marshmallow extract for 60 days in a preclinical experiment and then, challenged with a. hydrophila for a 10-day experiment. significant increases were observed in aspartate aminotransferase (ast), lactate dehydrogenase (ldh), alkaline phosphatase (al ... | 2017 | 28785391 |
| chronic endosulfan exposure impairs immune response rendering clarias gariepinus susceptible to microbial infection. | endosulfan, a persistent organochlorine insecticide affects several off-target organisms including fish though the underlying mechanisms remain obscure. in the present study, we monitored the effect of chronic endosulfan exposure on headkidney (hk), an important immune organ in fish and on fish immune system thereof. clarias gariepinus were exposed to a non-lethal concentration of endosulfan 2.884ppb (1/10th lc50) for 30 d which resulted in suppressed phagocytosis and bactericidal potential of h ... | 2017 | 28783490 |
| the effects of dietary inclusion of garlic on growth performance and disease resistance of african catfish (clarias gariepinus) fingerlings against aeromonas hydrophila infection. | the present study aimed to investigate the effects of dietary inclusion of garlic (allium sativum) peels and cloves on the growth performance and disease resistance of african catfish (clarias gariepinus) fingerlings against aeromonas hydrophila infection. seven isonitrogenous (36% protein) experimental diets were formulated to contain graded levels of garlic (peels and cloves) at 0, 10, 20 and 30g.kg-1. fish were fed twice a day for 12 weeks. the results demonstrated that no significant differe ... | 2016 | 28779743 |
| the effects of dietary inclusion of garlic on growth performance and disease resistance of african catfish (clarias gariepinus) fingerlings against aeromonas hydrophila infection. | the present study aimed to investigate the effects of dietary inclusion of garlic (allium sativum) peels and cloves on the growth performance and disease resistance of african catfish (clarias gariepinus) fingerlings against aeromonas hydrophila infection. seven isonitrogenous (36% protein) experimental diets were formulated to contain graded levels of garlic (peels and cloves) at 0, 10, 20 and 30g.kg-1. fish were fed twice a day for 12 weeks. the results demonstrated that no significant differe ... | 2016 | 28779742 |
| selection and evaluation of malaysian bacillus spp. strains as potential probiotics in cultured tiger grouper (epinephelus fuscoguttatus). | a total of 58 gram-positive bacteria strains were isolated from the marine environment and screened for potential probiotics for disease prevention and improving the productivity of tiger grouper epinephelus fuscoguttatus larvae and juveniles. the bacteria were identified as bacillus licheniformis, b. subtilis, b. circulans, b. sphaericus, b. cereus, brevibacillus brevis, corynebacterium propinquum, leifsonia aquatica and paenibacillus macerans. only 24 strains showed antagonistic activities aga ... | 2016 | 28779739 |
| inhibited fatty acid β-oxidation impairs stress resistance ability in nile tilapia (oreochromis niloticus). | energy metabolism plays important roles in stress resistance and immunity in mammals, however, such functions have not been established in fish. in the present study, nile tilapia (oreochromis niloticus) was fed with mildronate, an inhibitor of mitochondrial fatty acid (fa) β-oxidation, for six weeks subsequently challenged with aeromonas hydrophila and ammonia nitrogen exposure. mildronate treatment reduced significantly l-carnitine concentration and mitochondrial fa β-oxidation efficiency, whi ... | 2017 | 28774846 |
| activity of brazilian propolis against aeromonas hydrophila and its effect on nile tilapia growth, hematological and non-specific immune response under bacterial infection. | the effect of the ethanolic extract of propolis (eep) on aeromonas hydrophila was analyzed by determination of minimum inhibitory concentration (mic). then, the effects of crude propolis powder (cpp) on growth, hemato-immune parameters of the nile tilapia, as well as its effects on resistance to a. hydrophila challenge were investigated. the cpp (0.5, 1.0, 1.5, 2.0, 2.5 and 3.0%) was added to the diet of 280 nile tilapia (50.0 ± 5.7 g fish-1). hemato-immune parameters were analyzed before and af ... | 2017 | 28767889 |
| growth performance, innate immune responses and disease resistance of fingerling blunt snout bream, megalobrama amblycephala adapted to different berberine-dietary feeding modes. | a 8-week feeding trial was conducted to evaluate the effect of different berberine-dietary feeding modes on growth, non-specific immune responses and disease resistance of blunt snout bream, megalobrama amblycephala. fish (average initial weight 4.70 ± 0.02 g) were fed two fat levels (5% and 10%) diets in three berberine-feeding modes (supplementing 50 mg/kg berberine continuously, two-week or four-week intervals) with four replicates, respectively. then, fish were challenged by aeromonas hydrop ... | 2017 | 28754613 |
| antimicrobial property of lemongrass (cymbopogon citratus) oil against pathogenic bacteria isolated from pet turtles. | the usage of essential oils as antimicrobial agents is gaining attention. besides, pet turtles were known to harbor a range of pathogenic bacteria while the turtle keeping is a growing trend worldwide.the current study examined the antimicrobial activity of lemon grass oil (lgo) against seven species of gram negative bacteria namely; aeromonas hydrophila, a. caviae, citrobacter freundii, salmonella enterica, edwardsiella tarda, pseudomonas aeruginosa, and proteus mirabilis isolated from three po ... | 2017 | 28747972 |
| recovery of bacillus mycoides, b. pseudomycoides and aeromonas hydrophila from common carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss) with gill disease. | during a 3-month period from june to the end of august 2016, ~5% mortalities were observed in a farm with rainbow trout (oncorhynchus mykiss walbaum) and one farm of common carp (cyprinus carpio l.) in bulgaria. the disease was manifested by gill ulcers/rot, asphyxiation and bloody ascites. aeromonas hydrophila was isolated from the internal organs of all the diseased fish. bacillus mycoides or b. pseudomycoides were recovered from the gill lesions on diseased carp and rainbow trout, respectivel ... | 2017 | 28745820 |
| evaluation of in vivo probiotic efficiency of bacillus amyloliquefaciens in labeo rohita challenged by pathogenic strain of aeromonas hydrophila mtcc 1739. | diseases in aquatic organisms, including fish, are a major concern in aquaculture production. in this present investigation, we have evaluated the beneficial effects of dietary bacillus amyloliquefaciens ccf7 (genbank acc. no. kp256501) supplementation in rohu (labeo rohita) challenged by a pathogenic strain of aeromonas hydrophila mtcc 1739. four experimental diets were formulated: control diet (no probiotics) and three experimental diets (different concentrations of probiotic candidate b. amyl ... | 2017 | 28744833 |
| effect of cucurbita mixta (l.) seed meal enrichment diet on growth, immune response and disease resistance in oreochromis mossambicus. | the impact of cucurbita mixta (l.) seed meal enriched diet on growth performance, innate immune response, and disease resistance in oreochromis mossambicus against aeromonas hydrophila was investigated. o. mossambicus was fed with 2 g kg(-1), 4 g kg(-1), and 6 g kg(-1) c. mixta seed meal diets for a period of 4 weeks. the results indicated that c. mixta seed meal diets at 4 g kg(-1) and 6 g kg(-1) significantly (p < 0.05) enhances the survival rate, weight gain (wg), protein efficiency ratio (pe ... | 2017 | 28743630 |
| draft genome sequence of a multidrug-resistant aeromonas hydrophila st508 strain carrying rmtd and blactx-m-131 isolated from a bloodstream infection. | here we report the draft genome sequence of a multidrug-resistant (mdr) aeromonas hydrophila strain belonging to sequence type 508 (st508) isolated from a human bloodstream infection. assembly and annotation of this draft genome resulted in 5028498bp and revealed the presence of 16s rrna methylase rmtd and blactx-m-131 genes encoding high-level resistance to aminoglycosides and cephalosporins, respectively, as well as multiple virulence genes. this draft genome can provide significant informatio ... | 2017 | 28739226 |
| assessment of cultivable oral bacterial flora from important venomous snakes of india and their antibiotic susceptibilities. | snakebite is a common, frequently devastating, occupational, socio-economic hazard, and it has a great impact on the rural population of india. snakebite is a major cause of the human morbidity and mortality since ancient times, as it not only affects the victim by systemic envenomation but also by wound infections originating from deadly pathogenic microorganisms from the oral cavity of the offending snake. the pathogens from the oral cavity of the snake tend to initiate an infection, resulting ... | 2017 | 28733908 |
| in vitro assessment of the antimicrobial activity of silver and zinc oxide nanoparticles against fish pathogens. | antibiotic resistance is a global issue that threatens public health. the excessive use of antibiotics contributes to this problem as the genes of antibiotic resistance can be transferred between the bacteria in humans, animals and aquatic organisms. metallic nanoparticles could serve as future substitutes for some conventional antibiotics because of their antimicrobial activity. the aim of this study was to evaluate the antimicrobial effects of silver and zinc oxide nanoparticles against major ... | 2017 | 28732514 |
| microorganisms of the intestinal microbiota of oncorhynchus mykiss produce antagonistic substances against bacteria contaminating food and causing disease in humans. | the objective was to analyse the antibacterial ability of members of the intestinal microbiota of oncorhynchus mykiss specimens on several bacterial target strains that contaminate food and cause disease in humans. bacterial colonies from an intestinal portion of the 20 specimens of o. mykiss were obtained in different culture media. several of the colonies showed antibacterial action on different target strains. the bacterial species with the highest antagonistic capacity were hafnia alvei and ... | 2017 | 28713788 |
| aeromonas hydrophila-induced alterations in cytosolic calcium activate pro-apoptotic cpkc-mek1/2-tnfα axis in infected headkidney macrophages of clarias gariepinus. | alterations in intracellular-calcium (ca(2+))i homeostasis is critical to aeromonas hydrophila-induced headkidney macrophages (hkm) apoptosis of clarias gariepinus, though the implications are poorly understood. here, we describe the role of intermediate molecules of ca(2+-)signaling pathway that are involved in hkm apoptosis. we observed phosphoinositide-3-kinase/phospholipase c is critical for (ca(2+))i release in infected hkm. heightened protein kinase-c (pkc) activity and phosphorylation of ... | 2017 | 28713009 |
| transcriptome differences between enrofloxacin-resistant and enrofloxacin-susceptible strains of aeromonas hydrophila. | enrofloxacin is the most commonly used antibiotic to control diseases in aquatic animals caused by a. hydrophila. this study conducted de novo transcriptome sequencing and compared the global transcriptomes of enrofloxacin-resistant and enrofloxacin-susceptible strains. we got a total of 4,714 unigenes were assembled. of these, 4,122 were annotated. a total of 3,280 unigenes were assigned to go, 3,388 unigenes were classified into cluster of orthologous groups of proteins (cog) using blast and b ... | 2017 | 28708867 |
| rare bacterial isolates causing bloodstream infections in ethiopian patients with cancer. | in recent years, saprophytic bacteria have been emerging as potential human pathogens causing life-threatening infections in patients with malignancies. however, evidence is lacking concerning such bacteria, particularly in sub-saharan countries. this study was designed to determine the spectrum and drug resistance profile of the rare bacterial pathogens causing bloodstream infections (bsis) in febrile cancer patients at a referral hospital in ethiopia. | 2017 | 28702079 |
| dietary protein levels regulated antibacterial activity, inflammatory response and structural integrity in the head kidney, spleen and skin of grass carp (ctenopharyngodon idella) after challenged with aeromonas hydrophila. | this study investigated the effects of dietary protein levels on disease resistance, immune function and structural integrity in the head kidney, spleen and skin of grass carp (ctenopharyngodon idella). a total of 540 grass carp (264.11 ± 0.76 g) were fed six diets containing graded levels of protein (143.1, 176.7, 217.2, 257.5, 292.2 and 322.8 g digestible protein kg(-1) diet) for 8 weeks. after the growth trial, fish were challenged with aeromonas hydrophila for 14 days. the results indicated ... | 2017 | 28698127 |
| effects of lactobacillus delbrueckii on immune response, disease resistance against aeromonas hydrophila, antioxidant capability and growth performance of cyprinus carpio huanghe var. | the aim of the present study was to investigate effects of dietary lactobacillus delbrueckii (l. delbrueckii) on immune response, disease resistance against aeromonas hydrophila (a. hydrophila), antioxidant capability and growth performance of cyprinus carpio huanghe var. 450 fish (mean weight of 1.05 ± 0.03 g) were randomly distributed into five groups that fed diets containing different levels of l. delbrueckii (0, 1 × 10(5), 1 × 10(6), 1 × 10(7) and 1 × 10(8) cfu g(-1)) for 8 weeks. the resul ... | 2017 | 28698125 |
| immunotoxic responses of chronic exposure to cypermethrin in common carp. | in the current study, laboratory evaluations were made to assess the immunomodulatory effect of cypermethrin on fingerlings of common carp (cyprinus carpio l.). results showed that 96-h lc50 of cypermethrin in common carp was estimated at 0.85 μg/l. fish were exposed for 21 days to cypermethrin at three sub-lethal concentrations of 0.042, 0.085, and 0.17 μg/l that represented 5, 10, and 20%, respectively, of the 96-h lc50 of the pesticide for this fish species. blood samples were taken after 7, ... | 2017 | 28689359 |
| evaluation of dietary hybanthus enneaspermus (linn f. muell.) as a growth and haemato-immunological modulator in labeo rohita. | this study evaluated the effects of hybanthus enneaspermus aqueous extract (hee) as a feed supplement on growth, immune responses, haematological, and biochemical parameters of labeo rohita and its susceptibility to aeromonas hydrophila infection. diets containing five variable concentrations of hee (g kg(-1)) (0 [basal diet], 1.0 [h1], 2.0 [h2], 3.0 [h3], and 4.0 [h4]) were fed to fish (average weight: 17.2 ± 0.21 g) for 6 weeks. growth parameters, immune parameters, and haemato-biochemical par ... | 2017 | 28688911 |
| disruption of the quorum sensing regulated pathogenic traits of the biofilm-forming fish pathogen aeromonas hydrophila by tannic acid, a potent quorum quencher. | the quorum sensing (qs) phenomenon regulates a myriad of pathogenic traits in the biofilm forming fish pathogen, aeromonas hydrophila. blocking the qs mechanism of a. hydrophila is a novel strategy to prevent disease in fish. this study evaluated the effect of tannic acid, a qs inhibitor, on a. hydrophila-associated qs regulated phenomena. a streaking assay with chromobacterium violaceum (cvo26) reported the presence of n-acyl homoserine lactone (ahl) in a. hydrophila, which was confirmed by hpl ... | 2017 | 28685594 |
| quantitative proteomic analysis of iron-regulated outer membrane proteins in aeromonas hydrophila as potential vaccine candidates. | the iron-regulated outer membrane protein (omp) of aeromonas hydrophila is an effective vaccine candidate, but its intrinsic functional components are largely unknown. in this study, we compared the differentially expressed sarcosine-insoluble fractions of a. hydrophila in iron-limited and normal medium using tandem mass tag labeling-based quantitative proteomics, and identified 91 upregulated proteins including 21 omps and 83 downregulated proteins including 10 omps. subsequent bioinformatics a ... | 2017 | 28676336 |
| induction of cryptic and bioactive metabolites through natural dietary components in an endophytic fungus colletotrichum gloeosporioides (penz.) sacc. | grape skin and turmeric extracts having the major components resveratrol and curcumin, respectively, were used for the induction of cryptic and bioactive metabolites in an endophytic fungus colletotrichum gloeosporioides isolated from syzygium cumini. the increase in total amount of crude compounds in grape skin and turmeric extract treated cultures was 272.48 and 174.32%, respectively, compared to the untreated control. among six human pathogenic bacteria tested, the maximum inhibitory activity ... | 2017 | 28674526 |
| molecular characterization and expression pattern of x box-binding protein-1 (xbp1) in common carp (cyprinus carpio l.): indications for a role of xbp1 in antibacterial and antiviral immunity. | x box-binding protein-1 (xbp1) is a transcription factor that is essential for the unfolded protein response (upr) and the differentiation of plasma cells, and some findings have also uncovered its function in innate immunity. xbp1 typically has two different transcripts, un-spliced (xbp1u) and spliced forms (xbp1s), but xbp1s is an active transcription factor in the regulation of target genes. to date, there is no evidence about the identification and function of xbp1 in common carp. moreover, ... | 2017 | 28663129 |
| unusual severe extra-intestinal manifestations of a common enteric pathogen-aeromonas spp. | aeromonas spp. are halophilic, gram-negative bacilli. they are widely distributed in the soil and aquatic environment and have been associated with various extra-intestinal infections, such as skin and soft-tissue infections, meningitis, bacteraemia etc. the most common species associated with extra-intestinal infection is aeromonas hydrophila. | 2017 | 28658756 |
| coordination of bactericidal and iron regulatory functions of hepcidin in innate antimicrobial immunity in a zebrafish model. | hepcidin acts as both an antimicrobial peptide and a hormonal regulator of iron homeostasis; however, the biological significance of this dual-function in immune reactions remains elusive. in this study, we provide experimental evidence regarding the coordination of this dual-function in the innate antimicrobial immunity using a zebrafish model. the transcription of hepcidin gene was significantly upregulated in liver by aeromonas hydrophila (a.h) dna stimulation, which was accompanied by an inc ... | 2017 | 28655927 |
| transcriptome profiling and expression analysis of immune responsive genes in the liver of golden mahseer (tor putitora) challenged with aeromonas hydrophila. | transcriptome profiling has been used to decipher the novel mechanisms behind immune responses of the fishes. however, the molecular mechanism underlining immune response in mahseer is not studied so far. fishes are greatly affected by bacterial pathogens such as aeromonas hydrophila. in this study, transcriptome response of golden mahseer (tor putitora) infected with a. hydrophila was examined using paired end illumina sequencing of liver tissue to understand the immune response of the fish. th ... | 2017 | 28655594 |
| modulation of the innate immune responses in the striped snakehead murrel, channa striata upon experimental infection with live and heat killed aeromonas hydrophila. | it is well-known that the innate immune mechanisms in fish serve as the first line of defence against wide variety of pathogens. in most of the situations, innate responses get induced and enhanced after the pathogen invasion. it would be interesting to look into the inducibility of various innate immune mechanisms and the level of enhancement after infection with the pathogen. hence, in the present investigation, modulation of the innate immune responses in the striped snakehead murrel, channa ... | 2017 | 28652983 |
| a novel antimicrobial therapy for the control of aeromonas hydrophila infection in aquaculture using marine polysaccharide coated gold nanoparticle. | in the present study, we prepared fucoidan coated au-nps (fu-aunps), and examined its antimicrobial activity against aeromonas hydrophila. the green synthesized fu-aunps were bio-physically characterized by ultraviolet-visible (uv-vis) spectroscopy, x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), higher transmission electron microscopy (hr-tem), zeta potential analysis and energy dispersive x-ray spectroscopy (edx). fu-aunps were crystalline in nature, spherical to trian ... | 2017 | 28648622 |
| molecular characterization of the cd79a and cd79b and its role against aeromonas hydrophila infection in chinese sucker (myxocyprinus asiaticus). | cd79a and cd79b heterodimers are the important signaling components of b cell receptor (bcr) complex which plays a crucial role in b cell development and antibody production. in the present study, cd79a and cd79b homologues from chinese sucker (myxocyprinus asiaticus), namely macd79a and macd79b, were identified and their expression at early developmental stages and under constitutive and stimulated conditions were investigated. the cdna sequences for macd79a and macd79b contained open reading f ... | 2017 | 28647817 |
| transcriptome profiling and digital gene expression analysis of the skin of dybowski's frog (rana dybowskii) exposed to aeromonas hydrophila. | recently, populations of rana dybowskii, an important amphibian species in northeast china, have decreased, mainly owing to the disease caused by aeromonas hydrophila. however, effective control methods have not yet been developed. in order to explore the immune responses of r. dybowskii upon exposure to a. hydrophila infection, illumina high-throughput transcriptome sequencing and digital gene expression (dge) technology were employed to investigate transcriptomic changes in the skin of r. dybo ... | 2017 | 28647779 |
| impact of molecular hydrogen treatments on the innate immune activity and survival of zebrafish (danio rerio) challenged with aeromonas hydrophila. | recently, molecular hydrogen has been reported to have a suppressive effect on inflammation in human and rodent models. the aim of this study was to evaluate the preventive effects of hydrogen-rich water (hrw) on zebrafish challenged by a. hydrophila. we have found an increased survival rate of bacteria-challenged zebrafish subjected to the hrw immersion treatment. furthermore, we have revealed that hrw was able to block multiplication of a. hydrophila in zebrafish. in addition, treatment of zeb ... | 2017 | 28630014 |
| decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (ctenopharyngodon idella). | the current study explores the hypothesis that low or excess levels of lipids decrease the enteritis resistance ability through impairing the intestinal physical and immune barrier function of young grass carp (ctenopharyngodon idella). a total of 540 young grass carp with an average initial weight of 261.41 ± 0.53 g were fed diets containing six graded levels of lipids at 5.9, 21.4, 36.0, 50.2, 66.6 and 80.1 g/kg for 8 weeks. after that, a challenge trial was conducted by injection of aeromonas ... | 2017 | 28625872 |
| efficacy of plga microparticle-encapsulated formalin-killed aeromonas hydrophila cells as a single-shot vaccine against a. hydrophila infection. | control and prevention of disease is a high priority in aquaculture, and vaccination is important to prevent outbreaks. here, poly(d,l-lactide-co-glycolic acid) (plga) microparticles (mps) approximately 36μm in diameter were used to encapsulate and deliver aeromonas hydrophila formalin-killed cells (fkc) as an antigen, and the innate and adaptive immune responses of cyprinid loaches and common carp were assessed following vaccination. the antigen was confirmed to be well encapsulated by scanning ... | 2017 | 28623029 |
| draft genome sequence of aeromonas hydrophila strain bsk-10 (serotype o97), isolated from carassius carassius with motile aeromonad septicemia in china. | we report here a draft genome sequence of aeromonas hydrophila strain bsk-10, belonging to serotype o97, isolated from crucian carp (carassius carassius) with motile aeromonad septicemia in zhejiang, china. the assembly resulted in 34 scaffolds totaling approximately 4.97 mb, with an average g+c content of 60.97% and 4,594 predicted coding genes. | 2017 | 28619791 |
| haematological changes in south indian fresh water murrel, channa punctatus have both eus and a. hydrophila infection. | the mixed infection in fishes is a common feature and in this pathogens like bacteria, fungi and protozoan's are found together to cause ill health to the fishes known as epizootic ulcerative syndrome (eus). in this syndrome the low temperature and aquatic pollution aggravates the infection in fishes. in the present study the fresh water edible fish, channa punctatus (murrel) was found infected with the bacteria namely, aeromonas hydrophila, staphylococcus aureus, pseudomonas aeruginosa and salm ... | 2017 | 28615835 |
| dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (ctenopharyngodon idella). | in this study, we investigated the effects of dietary myo-inositol on the growth and intestinal physical barrier functions of young grass carp (ctenopharyngodon idella). a total of 540 young grass carp (221.83 ± 0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. after the growth trial, fish were challenged with aeromonas hydrophila for 14 days. the results indicated that compared with optimal myo-inositol levels, m ... | 2017 | 28610850 |
| the molecular characterizations of cu/znsod and mnsod and its responses of mrna expression and enzyme activity to aeromonas hydrophila or lipopolysaccharide challenge in qihe crucian carp carassius auratus. | superoxide dismutases (sods), as the prime antioxidant enzymes, present the first line of defense against oxidative stress caused by excessive reactive oxygen species (ros) in organism. in the study, two distinct members of sod family were cloned and analyzed in qihe crucian carp carassius auratus (designated as cacu/znsod and camnsod, respectively). the full-length cdna of cacu/znsod is 759 bp, containing a 5' -untranslated region (utr) of 39 bp, a orf (including stop codon, tag) of 465 bp and ... | 2017 | 28606861 |
| effects of partial replacement of fish meal by yeast hydrolysate on complement system and stress resistance in juvenile jian carp (cyprinus carpio var. jian). | a 10-week feeding trial was carried out to investigate the effects of dietary fish meal replacement by yeast hydrolysate (yh) on growth performance, complement system and stress resistance of juvenile jian carp (cyprinus carpio var. jian) (initial average weight 19.44 ± 0.06 g). in the study, there were five groups: one control group was fed with a basal diet (yh0), and four treatment groups were fed with dietary fish meal replaced by 1% yh (yh1), 3% (yh3), 5% (yh5) and 7% (yh7), respectively. e ... | 2017 | 28606860 |
| identification and characterization of a mannose-binding lectin from nile tilapia (oreochromis niloticus). | mannose-binding lectin (mbl) is a pattern recognition protein that plays an important role in innate immunity capable of activating the lectin pathway of the complement system. in this study, a mbl homologue (onmbl) was identified from nile tilapia (oreochromis niloticus) and characterized at expression and agglutination functional levels. the open reading frame of onmbl is 687 bp of nucleotide sequence encoding polypeptides of 228 amino acids. the deduced amino acid sequence is highly homology ... | 2017 | 28602737 |
| a c-type lectin, nattectin-like protein (cantc) in qihe crucian carp carassius auratus: binding ability with lps, pgn and various bacteria, and agglutinating activity against bacteria. | c-type lectins (ctls), as the members of pattern-recognition receptors (prrs), play the significant roles in innate immunity through binding with pathogen-associated molecular patterns (pamps) on the surface of microbe. in the present study, a novel ctl, nattectin-like protein (named as cantc), was investigated in qihe crucian carp carassius auratus. the full-length cdna of cantc was composed of 776 bp, with a 152 bp 5'-untranslated region (utr), a 492 bp orf encoding a 163-aa protein, and a 132 ... | 2017 | 28602683 |
| immune responses to methanolic extract of black cumin (nigella sativa) in rainbow trout (oncorhynchus mykiss). | the immune stimulating effects of the methanolic extract of black cumin (nigella sativa) in rainbow trout (oncorhynchus mykiss) was evaluated. variable concentrations of black cumin methanolic extract [0 (control), 0.1 and 0.5 g kg(-1) of feed] were individually added to the basal diet and rainbow trout was fed for 30 days to assess the innate immune responses and growth performance. feed conversion ratio significantly decreased in the group fed with 0.5 g kg(-1) black cumin extract. respiratory ... | 2017 | 28602677 |
| pattern recognition receptors in grass carp ctenopharyngodon idella: i. organization and expression analysis of tlrs and rlrs. | pattern recognition receptors (prrs) play indispensable roles in the immune responses against invading pathogens. in the present study, we systematically identified and characterized toll-like receptors (tlrs), retinoic acid-inducible gene i (rig-i)-like receptors (rlrs) as well as their adaptors in grass carp (ctenopharyngodon idella). a comprehensive analysis of blast and other bioinformatics methods showed that c. idella tlr family consist of 21 members and their adaptors contain four members ... | 2017 | 28559111 |
| dietary zinc deficiency reduced growth performance, intestinal immune and physical barrier functions related to nf-κb, tor, nrf2, jnk and mlck signaling pathway of young grass carp (ctenopharyngodon idella). | our study investigated the effects of dietary zinc (zn) deficiency on growth performance, intestinal immune and physical barrier functions of young grass carp (ctenopharyngodon idella). a total of 630 grass carp (244.14 ± 0.40 g) were fed graded levels of zinc lactate (10.71, 30.21, 49.84, 72.31, 92.56, 110.78 mg zn/kg diet) and one zinc sulfate group (56.9 mg zn/kg diet) for 60 days. at the end of the feeding trial, fish were challenged with aeromonas hydrophila for 14 days. these results indic ... | 2017 | 28549941 |
| melaleuca alternifolia essential oil prevents alterations to purinergic enzymes and ameliorates the innate immune response in silver catfish infected with aeromonas hydrophila. | aeromonas hydrophila infection represents a major impediment to the development of aquaculture, leading to important economic losses. over the last few years, different methods have been used to counteract and minimize the negative effects of this infection, such as the use of melaleuca alternifolia essential oil, popularly known as tea tree oil (tto), that possess a bactericide action against a. hydrophila. the purinergic system develops an important role in the inflammatory response, principal ... | 2017 | 28546114 |
| sodium butyrate improved intestinal immune function associated with nf-κb and p38mapk signalling pathways in young grass carp (ctenopharyngodon idella). | the present study evaluated the effect of dietary sodium butyrate (sb) supplementation on the growth and immune function in the proximal intestine (pi), middle intestine (mi) and distal intestine (di) of young grass carp (ctenopharyngodon idella). the fish were fed one powdery sodium butyrate (psb) diet (1000.0 mg kg(-1) diet) and five graded levels of microencapsulated sodium butyrate (msb) diets: 0.0 (control), 500.0, 1000.0, 1500.0 and 2000.0 mg kg(-1) diet for 60 days. subsequently, a challe ... | 2017 | 28546021 |
| molecular characterization and expression analysis of three tlr genes in yellow catfish (pelteobagrus fulvidraco): responses to stimulation of aeromonas hydrophila and tlr ligands. | toll-like receptors (tlrs) are one of the most extensively researched pattern recognition receptors (prrs) and play an important role in the innate immune system. in this study, partial cdna sequences of the pf_tlr18 and pf_tlr19 genes and complete cdna sequence of the pf_tlr21 gene were cloned from yellow catfish (pelteobagrus fulvidraco). the open reading frames (orfs) of the pf_tlr18, pf_tlr19 and pf_tlr21 genes were 1956 bp, 2262 bp and 2949 bp in length, encoding 651, 753 and 982 amino acid ... | 2017 | 28546018 |
| selection and characterization of alanine racemase inhibitors against aeromonas hydrophila. | combining experimental and computational screening methods has been of keen interest in drug discovery. in the present study, we developed an efficient screening method that has been used to screen 2100 small-molecule compounds for alanine racemase alr-2 inhibitors. | 2017 | 28545531 |
| comparative analysis of two ferritin subunits from blunt snout bream (megalobrama amblycephala): characterization, expression, iron depriving and bacteriostatic activity. | iron is an essential microelement for almost all living organisms, while an excess of iron is toxic, thus maintenance of iron homeostasis is vital. as iron storage protein, ferritin plays an important role in iron metabolism. in the present study, we cloned and characterized the ferritin h subunit from megalobrama amblycephala, termed as mamferh. an iron-responsive element (ire) was predicted in the 5' untranslated region (utr) of mamferh, while its bulge structural was different from that of th ... | 2017 | 28535971 |
| evaluation of three recombinant outer membrane proteins, ompa1, tdr, and tbpa, as potential vaccine antigens against virulent aeromonas hydrophila infection in channel catfish (ictalurus punctatus). | a virulent clonal population of aeromonas hydrophila (vah) is recognized as the etiological agent in outbreaks of motile aeromonas septicemia (mas) in catfish aquaculture in the southeastern united states since 2009. genomic subtraction revealed three outer membrane proteins present in vah strain ml09-119 but not in low virulence reference a. hydrophila strains: major outer membrane protein ompa1, tonb-dependent receptor (tdr), and transferrin-binding protein a (tbpa). here, the genes encoding o ... | 2017 | 28532667 |